A hair loss prevention and growth composition based on Platycladus orientalis compound extract and its preparation method
The hair loss prevention and hair growth composition prepared by the preparation method of Platycladus orientalis compound extract, combined with fermentation and alcohol extraction technology, solves the side effect problem of Western medicine treatment of androgenic alopecia, and achieves an effective treatment effect without irritation and toxicity.
Patent Information
- Application Number
- CN202510180005.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-19
- Publication Date
- 2025-10-03
- Estimated Expiration
- 2045-02-19
AI Technical Summary
Existing Western medicines for the treatment of androgenic alopecia have side effects and are not ideal in effect, and Chinese herbal extract products are insufficiently used in this field.
The preparation method of Platycladus orientalis compound extract is adopted. By mixing Platycladus orientalis leaves, Forsythia suspensa, Ligustrum lucidum fruit, Cuscuta australis and other Chinese medicinal materials in specific proportions, combined with fermentation and alcohol extraction technology, a hair loss and hair growth composition is prepared. The synergistic effect of the main drug, ministerial drug, adjuvant drug and guiding drug is utilized to improve the scalp environment and blood circulation and inhibit α-reductase.
It effectively improves androgenic alopecia, reduces scalp inflammation, promotes hair growth, inhibits 5α-reductase, and reduces dihydrotestosterone production without irritation or toxic side effects.
Smart Images
Figure CN119818616B_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the technical field of external skin compositions, and particularly relates to a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract and a preparation method thereof. Background Art
[0002] Hair loss, also known as hair loss or baldness, is a common health problem that can be caused by a variety of reasons and has different types. Androgenetic alopecia is the most common type of hair loss and is often related to genetics and hormone levels. It is more common in men and is known as "male pattern baldness."
[0003] Currently, the following are the main Western medications for androgenic alopecia: 1. Minoxidil, which dilates blood vessels, increases blood flow to the scalp, and promotes nutrient supply to hair follicles, thereby stimulating hair growth. Long-term use is required to maintain the effect; upon discontinuation of use, new hair may fall out again. It may cause local side effects such as dryness, itching, and redness of the scalp. 2. Finasteride, an oral medication, inhibits 5α-reductase, reducing the production of dihydrotestosterone (DHT), thereby slowing the progression of hair loss. It is primarily suitable for men; women should use it with caution as it may cause serious side effects. It may also cause sexual dysfunction and other side effects.
[0004] Traditional Chinese medicine extracts offer numerous advantages for treating hair loss, including fewer toxic side effects than chemically synthesized drugs. Traditional Chinese medicine emphasizes a holistic approach, focusing not only on the site of hair loss but also on the overall health of the body, such as qi and blood, liver and kidney function, to achieve therapeutic goals by regulating systemic functions. Therefore, Western medications such as minoxidil and finasteride have been widely used in the treatment of androgenic alopecia, but they have limitations and side effects. In contrast, traditional Chinese medicine extracts, with their natural, comprehensive, personalized, and less dependent properties, compensate for the shortcomings of Western medicine to a certain extent. However, currently available products for androgenic alopecia are less than ideal.
[0005] Therefore, there is an urgent need for a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract and a preparation method thereof. Summary of the Invention
[0006] The present invention aims to provide a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract and a preparation method thereof.
[0007] In order to achieve the above object, the present invention provides the following technical solutions:
[0008] A method for preparing a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract, the preparation method comprising the following steps: by weight,
[0009] (1) 10 parts of Platycladus orientalis leaves, 13-16 parts of Forsythia suspensa, 12-15 parts of Ligustrum lucidum fruits, and 3-6 parts of Cuscuta seeds were mixed and crushed to obtain raw material A;
[0010] (2) mixing raw material A and water in a weight ratio of 1:(6-8) to obtain a mixture, adding Lactobacillus plantarum to the mixture, fermenting, sterilizing after fermentation, filtering, and obtaining an extract A;
[0011] (3) mixing 18-22 parts of snakehead intestine, 2-5 parts of sophora flavescens, 15-17 parts of gentian, and 6-10 parts of dendrobium officinale, and grinding them to obtain raw material B;
[0012] (4) mixing raw material B and ethanol aqueous solution in a weight ratio of 1:(6-8), heating and refluxing for extraction, filtering to obtain a filtrate, and subjecting the filtrate to reduced pressure rotary evaporation to recover ethanol, to obtain the remaining liquid as extract B;
[0013] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.
[0014] The present invention utilizes a composition prepared by carefully selecting specific components and using specific proportions to improve androgenic alopecia. The main ingredients include Platycladus orientalis leaves and Forsythia suspensa. Their efficacy is antibacterial and anti-inflammatory, promoting hair growth and darkening, and preventing hair loss. Mechanism of action: These herbs possess antibacterial and anti-inflammatory properties, reducing scalp inflammation and promoting hair growth. The auxiliary ingredients include Cuscuta seeds and Ligustrum lucidum fruit. Their efficacy is tonifying Qi and nourishing the liver and kidneys. Mechanism of action: These herbs help replenish Qi and blood, nourish the liver and kidneys, thereby improving the nutritional supply of hair and enhancing its growth potential. The adjuvant ingredients include Echinops intestinalis and Sophora flavescens. Their efficacy is promoting blood circulation. Their efficacy is promoting blood circulation and increasing blood supply to the scalp, thereby improving the nutritional status of hair follicles and promoting hair growth. The auxiliary ingredients include Gentiana scabra and Dendrobium officinale. Their efficacy is calming and nourishing. Mechanism of action: These herbs help calm the mind and nourish blood, reducing the effects of stress on hair while improving overall health, thereby indirectly promoting hair growth. Through the combination of these Chinese medicinal materials, the scalp environment can be improved in many aspects, inflammation can be reduced, blood circulation can be promoted, and nutrition can be supplemented, thereby effectively treating androgenic alopecia.
[0015] Furthermore, in step (1), the powder is crushed to a size less than 100 mesh.
[0016] Furthermore, the fermentation condition in step (2) is 35-40° C. for 10-12 hours.
[0017] Furthermore, in step (2), 0.22 μm filter membrane is used for filtration.
[0018] Furthermore, in step (3), the powder is crushed to a size of less than 300 mesh.
[0019] Furthermore, the volume concentration of the ethanol aqueous solution in step (4) is 55-60%.
[0020] Furthermore, the time of heating reflux extraction in step (4) is 2-3 hours.
[0021] Furthermore, in step (4), 0.22 μm filter membrane is used for filtration.
[0022] The present invention improves the α-reductase inhibition rate by dividing the raw materials into two groups and performing fermentation and alcohol extraction respectively, thereby effectively treating androgenic alopecia. Ingredients such as Platycladus orientalis leaves, Forsythia suspensa, Ligustrum lucidum fruit, and Cuscuta australis may contain precursor compounds that require biotransformation to better release active substances. By using Lactobacillus plantarum for fermentation, it is possible to promote the conversion of certain compounds in these ingredients into more easily absorbed or more active forms. Ingredients such as Echinops ulmoides, Sophora flavescens, Gentiana scabra, and Dendrobium officinale may be rich in fat-soluble or heat-stable active ingredients, which are more suitable for extraction by heating and refluxing with ethanol-water solution.
[0023] The present invention also provides a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract prepared by the preparation method.
[0024] Compared with the prior art, the advantages and beneficial effects of the present invention are:
[0025] 1. The present invention can improve androgenic alopecia by selecting specific components and preparing a composition in a specific ratio.
[0026] 2. The present invention can improve the α-reductase inhibition rate by dividing the raw materials into two groups and performing fermentation and alcohol extraction respectively, thereby effectively treating androgenic alopecia. BRIEF DESCRIPTION OF THE DRAWINGS
[0027] Figure 1 This is the acute dermal toxicity test report.
[0028] Figure 2 This is the test report of 5α-reductase inhibition test.
[0029] Figure 3 This is a before-and-after comparison picture of Volunteer 1.
[0030] Figure 4 This is a before-and-after comparison photo of Volunteer 2.
[0031] Figure 5 This is a before-and-after comparison photo of Volunteer No. 3.
[0032] Figure 6This is a before-and-after comparison photo of volunteer No. 4. DETAILED DESCRIPTION
[0033] The following is a clear and complete description of the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection of the present invention.
[0034] The raw materials used in the following examples of the present invention are all commercially available commodities:
[0035] Lactobacillus plantarum, product number CCTCC LB 2008168, was purchased from China Center for Type Culture Collection.
[0036] Lactobacillus bulgaricus, product number CCTCC CB 20082296, was purchased from China Center for Type Culture Collection.
[0037] Example 1
[0038] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by parts by weight,
[0039] (1) 10 parts of Platycladus orientalis leaves, 14 parts of Forsythia suspensa, 13 parts of Ligustrum lucidum fruits, and 5 parts of Cuscuta seeds were mixed and crushed to less than 100 mesh to obtain raw material A;
[0040] (2) Mixing raw material A and water in a weight ratio of 1:7 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 38°C for 11 hours. After fermentation, sterilize the mixture and filter it through a 0.22 μm filter membrane to obtain extract A;
[0041] (3) 20 parts of snakehead intestine, 4 parts of sophora flavescens, 16 parts of gentian, and 7 parts of dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;
[0042] (4) Mixing raw material B with a volume concentration of 58% ethanol aqueous solution at a weight ratio of 1:7, heating and refluxing for 2.5 h, filtering with a 0.22 μm filter membrane to obtain a filtrate, and vacuum-evaporating the filtrate to recover ethanol, obtaining the remaining liquid as extract B;
[0043] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.
[0044] Example 2
[0045] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by parts by weight,
[0046] (1) 10 parts of Platycladus orientalis leaves, 13 parts of Forsythia suspensa, 15 parts of Ligustrum lucidum fruits, and 3 parts of Cuscuta seeds were mixed and crushed to less than 100 mesh to obtain raw material A;
[0047] (2) Mixing raw material A and water in a weight ratio of 1:6 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 40° C. for 10 h. After fermentation, sterilize the mixture and filter it using a 0.22 μm filter membrane to obtain extract A;
[0048] (3) 22 parts of snakehead intestine, 2 parts of sophora flavescens, 17 parts of gentian, and 6 parts of dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;
[0049] (4) Mixing raw material B with a volume concentration of 55% ethanol aqueous solution at a weight ratio of 1:6, heating and refluxing for 3 h, filtering with a 0.22 μm filter membrane to obtain a filtrate, and vacuum-evaporating the filtrate to recover ethanol, obtaining the remaining liquid as extract B;
[0050] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.
[0051] Example 3
[0052] This embodiment provides a method for preparing a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by parts by weight,
[0053] (1) 10 parts of Platycladus orientalis leaves, 16 parts of Forsythia suspensa, 12 parts of Ligustrum lucidum fruits, and 6 parts of Cuscuta seeds were mixed and crushed to less than 100 mesh to obtain raw material A;
[0054] (2) Mixing raw material A and water in a weight ratio of 1:8 to obtain a mixture, adding Lactobacillus plantarum to the mixture, and fermenting the mixture at 35°C for 12 hours. After fermentation, sterilize the mixture and filter it through a 0.22 μm filter membrane to obtain extract A;
[0055] (3) 18 parts of snakehead intestine, 5 parts of sophora flavescens, 15 parts of gentian, and 10 parts of dendrobium officinale were mixed and crushed to less than 300 mesh to obtain raw material B;
[0056] (4) Mixing raw material B with a volume concentration of 60% ethanol aqueous solution at a weight ratio of 1:8, heating and refluxing for 2 h, filtering with a 0.22 μm filter membrane to obtain a filtrate, and vacuum-evaporating the filtrate to recover ethanol, obtaining the remaining liquid as extract B;
[0057] (5) The extract A and the extract B are mixed to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.
[0058] Comparative Example 1
[0059] The difference between this comparative example and Example 1 is that 10 parts of Platycladus orientalis leaves, 13 parts of Forsythia suspensa, 15 parts of Ligustrum lucidum fruits, and 3 parts of Cuscuta seeds are replaced by 13 parts of Platycladus orientalis leaves, 10 parts of Forsythia suspensa, 3 parts of Ligustrum lucidum fruits, and 15 parts of Cuscuta seeds.
[0060] Comparative Example 2
[0061] The difference between this comparative example and Example 1 is that the Ligustrum lucidum fruit is replaced by Scutellaria baicalensis.
[0062] Comparative Example 3
[0063] The difference between this comparative example and Example 1 is that 20 parts of snakehead intestines, 4 parts of sophora flavescens, 16 parts of gentian and 7 parts of dendrobium officinale are replaced by 12 parts of snakehead intestines, 12 parts of sophora flavescens, 7 parts of gentian and 16 parts of dendrobium officinale.
[0064] Comparative Example 4
[0065] The difference between this comparative example and Example 1 is that Gentiana scabra is replaced by Eucommia ulmoides.
[0066] Comparative Example 5
[0067] The difference between this comparative example and Example 1 is that Lactobacillus plantarum is replaced by Lactobacillus bulgaricus.
[0068] Comparative Example 6
[0069] The difference between this comparative example and Example 1 is: a preparation method of a hair loss prevention and hair growth composition based on a compound extract of Platycladus orientalis, the preparation method comprising the following steps: by weight,
[0070] (1) 10 parts of Platycladus orientalis leaves, 14 parts of Forsythia suspensa, 13 parts of Ligustrum lucidum fruits, 5 parts of Cuscuta seeds, 20 parts of Echinops cholerae, 4 parts of Sophora flavescens, 16 parts of Gentiana scabra, and 7 parts of Dendrobium officinale were mixed and crushed to less than 100 mesh to obtain a raw material;
[0071] (2) The raw materials in a weight ratio of 1:7 and a 58% ethanol aqueous solution were mixed, heated under reflux for extraction for 2.5 hours, and filtered using a 0.22 μm filter membrane to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract.
[0072] Performance Testing
[0073] The hair loss prevention and hair growth compositions based on Platycladus orientalis compound extract prepared in Examples 1-3 and Comparative Examples 1-6 were used as samples for performance testing.
[0074] 1. According to Chapter 6 of the "Technical Specifications for Safety of Cosmetics" (2015 edition), multiple skin irritation tests were conducted on the samples, with 4 New Zealand rabbits (ordinary grade, female, weighing 2.0-2.5kg) in each group. Animal Certificate Number: NO.44411600012896.
[0075] The results showed that the reaction scores of erythema and edema of each animal in each group of samples were 0 on day 14, indicating that the compositions prepared in Examples 1-3 and Comparative Examples 1-6 were non-irritating.
[0076] 2. According to Figure 1 Acute percutaneous toxicity tests were conducted on the samples using the method described in the preceding text. The results showed that the animals in each group showed no symptoms of poisoning and no deaths occurred within 14 days of exposure. The animals' weights were normal, and no abnormalities were found in the gross autopsies performed after the exposure. This indicates that the compositions prepared in Examples 1-3 and Comparative Examples 1-6 are non-toxic. Figure 1 This is the test report of the sample in Example 1.
[0077] 3. The 5α-reductase inhibition test is mainly used to evaluate whether the activity of 5α-reductase can be effectively inhibited. This enzyme is one of the key factors in androgenic alopecia. Androgenic alopecia is related to increased levels of dihydrotestosterone (DHT) in the body, and DHT is converted from testosterone under the action of 5α-reductase. Therefore, by inhibiting 5α-reductase, the production of DHT can be reduced, thereby slowing down the process of hair loss. Figure 2 The 5α-reductase inhibition assay was performed using the method described in
[15] .
[0078] The results are shown in Table 1. Figure 2 This is the test report of the sample in Example 1.
[0079] Table 1. 5α-reductase inhibition rate test results
[0080]
[0081]
[0082] From the above performance test results, it can be seen that the hair loss prevention and hair growth compositions based on the Platycladus orientalis compound extract of Examples 1-2 are non-irritating, non-toxic, and have good α-reductase inhibition effects, especially the comprehensive performance of Example 1 is the most outstanding.
[0083] However, the comparative example does not adopt the necessary technical solutions, resulting in its performance being significantly worse than that of the embodiment in the corresponding performance tests.
[0084] In Comparative Example 1, the ratio of Platycladus orientalis leaves, Forsythia suspensa, Ligustrum lucidum fruit and Cuscuta australis was changed, and it can be seen that the inhibitory effect decreased, which proves that the components of the specific formula are fermented and play a synergistic role with each other during the fermentation process, and more abundant active components can be extracted to inhibit α-reductase.
[0085] In Comparative Example 2, Ligustrum lucidum fruit was replaced with Scutellaria baicalensis, and it can be seen that the inhibitory effect decreased, indicating that the prescriptions of the present invention have a synergistic effect with each other, and the effect decreases if the raw materials are replaced at will.
[0086] In Comparative Example 3, the ratio of Echinops intestinalis, Sophora flavescens, Gentiana scabra, and Dendrobium officinale was changed, and it can be seen that the inhibitory effect decreased, which proves that the components of a specific formula can be extracted to extract more active components and inhibit α-reductase.
[0087] In Comparative Example 4, Gentiana scabra was replaced with Eucommia ulmoides. It can be seen that the inhibitory effect decreased, indicating that the prescriptions of the present invention have a synergistic effect with each other. If the raw materials are replaced arbitrarily, the effect will decrease.
[0088] In Comparative Example 5, different bacteria were used for fermentation, and it can be seen that the inhibitory effect decreased, which proves that the active components prepared by the present invention using specific bacteria for fermentation are more conducive to inhibiting α-reductase.
[0089] In Comparative Example 6, all components were mixed and then subjected to alcohol extraction. It can be seen that the inhibitory effect decreased, indicating that the extraction rate of the active component of this extraction method is low.
[0090] 4. The hair loss prevention and hair growth composition based on Platycladus orientalis compound extract prepared in Example 1 was mixed with water in a volume ratio of 1:1 and used. Four volunteers with androgenic alopecia were randomly selected and 2 mL was evenly applied to the entire scalp once a day for 3 consecutive months. The results were shown in Table 1. Figure 3-6 .
[0091] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.
Claims
1. A method for preparing a hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract, characterized in that: The preparation method comprises the following steps: by weight, (1) 10 parts of Platycladus orientalis leaves, 13-16 parts of Forsythia suspensa, 12-15 parts of Ligustrum lucidum fruits, and 3-6 parts of Cuscuta seeds were mixed and crushed to obtain raw material A; (2) mixing raw material A and water to obtain a mixture, adding Lactobacillus plantarum to the mixture, fermenting, sterilizing, and filtering to obtain extract A after the fermentation is complete; (3) mixing 18-22 parts of snakehead intestine, 2-5 parts of sophora flavescens, 15-17 parts of gentian, and 6-10 parts of dendrobium officinale, and grinding them to obtain raw material B; (4) mixing the raw material B and the ethanol aqueous solution, heating and refluxing for extraction, filtering to obtain a filtrate, and subjecting the filtrate to vacuum rotary evaporation to recover the ethanol, to obtain the remaining liquid as the extract B; (5) mixing the extract A and the extract B to obtain a hair loss prevention and hair growth composition based on the Platycladus orientalis compound extract; The fermentation conditions in step (2) are 35-40° C. for 10-12 hours; The volume concentration of the ethanol aqueous solution in step (4) is 55-60%, and the heating reflux extraction time is 2-3 hours.
2. The method for preparing the anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (2), raw material A and water are mixed in a weight ratio of 1:(6-8).
3. The method for preparing the anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (1), the powder is crushed to a size of less than 100 mesh.
4. The method for preparing the anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (2), a 0.22 μm filter membrane is used for filtration.
5. The method for preparing the anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (3), the powder is crushed to a size of less than 300 mesh.
6. The method for preparing the anti-hair loss and hair growth composition based on the Platycladus orientalis compound extract according to claim 1, characterized in that: In the step (4), a 0.22 μm filter membrane is used for filtration.
7. A hair loss prevention and hair growth composition based on a Platycladus orientalis compound extract prepared by the preparation method according to any one of claims 1 to 6.