Method for increasing extraction rate of sinomenine in caulis sinomenii
By extruding and standing the underground stem of Qingfeng Vine, its internal structure is changed, and the problems of low extraction rate and high cost in the prior art are solved, and efficient extraction and production efficiency of Qingfeng Vine are achieved.
Patent Information
- Application Number
- CN202510218359.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-26
- Publication Date
- 2025-04-15
AI Technical Summary
In the existing cyperon extraction technology, the extraction rate is low, the cost is high, the equipment is complex, and the conventional pretreatment method is not conducive to the full extraction of cyperon.
By extruding the underground stem of the green feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng feng
It significantly improves the extraction rate of 陈号, which is simple to operate and low cost, which helps to obtain more 陈号 in the production process and improves production efficiency.
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Abstract
Description
Technical Field
[0001] The invention relates to a method for improving the extraction rate of sinomenine in Caulis Sinomenii, and belongs to the technical field of traditional Chinese medicine extraction technology. Background Art
[0002] Caulis Sinomenii is the stem of the vine of Caulis Sinomenii or Caulis Sinomenii of the Menispermaceae family. It plays an important role in traditional medicine. Sinomenine is an important alkaloid component in Caulis Sinomenii. From the chemical structure point of view, sinomenine has unique structural characteristics, which makes it have multiple pharmacological activities. In terms of medicinal value, sinomenine has anti-inflammatory effects, can effectively reduce inflammatory reactions, and has a significant effect on improving symptoms of some inflammatory diseases such as rheumatoid arthritis. It also has analgesic effects and can relieve a variety of pains, whether it is neuropathic pain or inflammatory pain. In addition, sinomenine also plays a positive role in immunoregulation, can regulate the body's immune function, and has potential application value in the treatment of autoimmune diseases.
[0003] At present, the main method for obtaining sinomenine is solvent extraction. For example, ethanol and other organic solvents are used to reflux extract Caulis Sinomenii. First, after crushing Caulis Sinomenii, add an appropriate amount of ethanol, reflux at a certain temperature for a certain time, and then filter, concentrate and other steps to obtain an extract containing sinomenine. In addition, there is an acid-water extraction method, which uses an acidic aqueous solution to extract sinomenine from Caulis Sinomenii, and then further purifies it through operations such as alkalization and organic solvent extraction to obtain sinomenine. In order to improve the extraction rate of sinomenine, existing means include optimizing the selection of extraction solvents. For example, mixed solvents of different proportions are studied to try to find the solvent system that is most conducive to the dissolution of sinomenine. There is also optimization of extraction process parameters, such as adjusting the extraction temperature, time and material-liquid ratio. However, these methods have failed to increase the content of sinomenine from the root. The use of excessive organic solvents in the solvent extraction method is not only flammable and explosive, complex to recover, and high cost, but also the conventional pre-treatment method of Caulis Sinomenii is not conducive to the full extraction of sinomenine. A large amount of organic solvents are required for dissolution, and the dissolved content is also limited. In the acid water extraction method, acid water corrodes the equipment and sinomenine is easily lost in the subsequent steps. Conventional pretreatment methods cannot fully extract sinomenine from the inside of the vine stems. In terms of crushing Caulis Sinensis, there are also studies using different crushing methods, such as mechanical crushing, ultrasonic crushing, etc., the purpose is to increase the contact area between Caulis Sinensis and the solvent, thereby improving the extraction rate of sinomenine. Although mechanical crushing can increase the contact area, it may cause local overheating and destroy the structure of sinomenine; although ultrasonic crushing can improve efficiency, the equipment cost is relatively high, and the stability during large-scale production needs to be improved. In summary, the existing sinomenine extraction technical means need to be further improved to improve the extraction rate of sinomenine and reduce costs. Summary of the invention
[0004] In view of the defects existing in the prior art, the purpose of the present invention is to provide a method for improving the extraction rate of sinomenine in Caulis Sinomenii. The present invention extrudes the underground stems of Caulis Sinomenii to change its internal structure, and allows it to stand for further transformation and distribution adjustment of sinomenine in the plant body, thereby increasing the content of sinomenine. The method is simple to operate, low in cost, and can significantly improve the extraction rate of sinomenine, which helps to obtain more sinomenine in the production process, thereby improving production efficiency.
[0005] In order to achieve the above technical objectives, the present invention provides a method for improving the extraction rate of sinomenine in Caulis Sinensis, which comprises washing the harvested Caulis Sinensis underground stems, cutting them into cylindrical segments I of equal length and subjecting them to extrusion treatment, leaving them to stand under indoor ventilation conditions, slicing them again and drying them; the extrusion treatment is to apply pressure perpendicular to the axial direction of the Caulis Sinensis cylindrical segments I of equal length, so that the side thickness is compressed to become cylindrical segments II.
[0006] The technical solution of the present invention processes Caulis Sinomenii through multiple steps to change the physical and chemical state of Caulis Sinomenii, thereby promoting the improvement of sinomenine content. The extraction amount of sinomenine can be significantly improved. The method is simple and easy to implement, low in cost, and provides a way to improve the subsequent extraction rate of sinomenine. Due to the distribution of the internal tissue structure of Caulis Sinomenii, the cylindrical Caulis Sinomenii segment is vertically squeezed in the axial direction, which can evenly destroy the tissue and structure of sinomenine, thereby promoting the aerobic bacteria inside the sinomenine to obtain more oxygen, so that the sinomenine precursor is converted, and then the sinomenine content is increased and more sinomenine is released, creating more favorable conditions for the subsequent improvement of sinomenine content and sinomenine extraction. After the extrusion treatment, the internal structure of Caulis Sinomenii is destroyed, and a large amount of oxygen can enter the interior of the plant. Further, placing it in a well-ventilated indoor environment and letting it stand can naturally dissipate the moisture inside the Caulis Sinomenii, and help the internal components to stabilize after extrusion, which lays the foundation for subsequent steps such as slicing and drying, and is conducive to improving the overall efficiency of sinomenine extraction. Through the synergistic effect of these two key steps, combined with other pretreatment, re-slicing and drying operations, the extraction rate of sinomenine can be effectively improved, ensuring that sinomenine can be present in sufficient quantity and good quality in subsequent applications.
[0007] As a preferred solution, the side thickness of the cylindrical segment II is 0.4 to 0.8 times the side thickness of the cylindrical segment I.
[0008] During the extrusion process, this ratio range can ensure that enough tissue structures are destroyed, and improve the contact between air and aerobic bacteria in the tissue structure of Caulis Sinensis. Aerobic bacteria can continue to consume the precursor of sinomenine and convert it into sinomenine under the premise of sufficient oxygen. If the ratio is too high, it means that the degree of extrusion is not enough, the tissue structure is not fully destroyed, and it is difficult for air to enter and combine with the endophytic bacteria in Caulis Sinensis to continue to produce sinomenine, which will lead to a decrease in its extraction rate; if the ratio is too low, that is, excessive compression, it may excessively damage the tissue structure and destroy the balance between aerobic and anaerobic bacteria. At the same time, sinomenine is unnecessarily destroyed or mixed with other impurities more seriously, which is not conducive to improving the extraction rate.
[0009] As a preferred solution, the length of the cylindrical section I is 6 to 14 cm.
[0010] As a preferred solution, the standing time is 3 to 8 days.
[0011] As a preferred solution, the standing temperature is 15-25°C.
[0012] In the process of extracting sinomenine from Caulis Sinomenii, the principle of the standing step is based on the characteristics of biochemical reactions and microbial activities inside Caulis Sinomenii. The temperature of 15~25℃ is conducive to the natural dissipation of water inside Caulis Sinomenii and the stability of the internal components of Caulis Sinomenii. In this temperature range, the biochemical reactions related to sinomenine inside Caulis Sinomenii can proceed normally, and the microbial activities are in a relatively controllable state. The high temperature will not cause the microorganisms to multiply in large numbers and affect the quality of sinomenine, nor will the low temperature cause the water to dissipate too slowly and the adjustment of internal components to be hindered. If the temperature is too high, the microorganisms will reproduce faster, which may decompose sinomenine or produce other impurities; if the temperature is too low, the water will dissipate slowly, and the internal component stabilization process will be hindered, which is not conducive to subsequent extraction operations.
[0013] The present invention can give aerobic bacteria enough time to produce sinomenine by standing for 3 to 8 days. It takes a certain amount of time for aerobic bacteria to grow and reproduce inside Caulis Sinomenii. During this period of 3 to 8 days, aerobic bacteria can fully carry out activities such as metabolism in a suitable environment (such as suitable temperature, air and other conditions). In this process, the activity of aerobic bacteria contributes to the production of sinomenine. At the same time, this period of time can also allow the composition of Caulis Sinomenii to be adjusted and stabilized in the state after extrusion treatment, laying a good foundation for subsequent extraction operations. If the standing time is too short, aerobic bacteria do not have enough time to carry out related activities, and the amount of sinomenine produced may be insufficient; if the standing time is too long, excessive growth and reproduction of microorganisms may occur, leading to mildew, which may cause sinomenine to be decomposed or contaminated by other impurities, which is not conducive to the improvement of sinomenine extraction rate and quality assurance.
[0014] As a preferred solution, the drying temperature is 70-100°C.
[0015] While effectively removing moisture from Caulis Sinomenii, the activity and stability of sinomenine are ensured. Within this temperature range, the moisture can be evaporated quickly, so that the Caulis Sinomenii reaches a dry state suitable for extracting sinomenine. If the temperature is lower than 70°C, the drying speed is too slow, which will extend the drying time, increase costs, and may cause problems such as microbial contamination of Caulis Sinomenii during the drying process; if the temperature is higher than 100°C, it will increase unnecessary energy consumption and increase costs.
[0016] As a preferred solution, the drying time is 4 to 13 hours.
[0017] If the drying time is too short, the Caulis Sinomenii may not reach the required degree of dryness and the moisture content may be too high, which will affect the extraction rate of sinomenine and may cause problems such as mildew during subsequent storage. If the drying time is too long, it may be over-dried at high temperature, destroying the structure of sinomenine or causing unnecessary changes to other components of the Caulis Sinomenii, affecting the quality of sinomenine.
[0018] As a preferred solution, the moisture content after drying is less than 13.0%.
[0019] In order to ensure the stability of the sinomenine extraction process and the quality of sinomenine, a lower moisture content can reduce the impact of moisture on the extraction reagents or extraction equipment during the extraction process, and prevent the sinomenine from mildew or other chemical reactions during storage and subsequent processing. If the moisture content is too high, it will not only affect the extraction rate of sinomenine, but also cause sinomenine to deteriorate during storage, reducing its medicinal value or industrial utilization value.
[0020] Compared with the prior art, the technical solution of the present invention brings about the following innovative and beneficial technical effects: 1) The present invention processes Caulis Sinomenii in multiple steps and extrude Caulis Sinomenii of a specific length, so that the thickness of the compressed side surface is maintained at 0.4 to 0.8 times of the original thickness. This precise extrusion process can facilitate the subsequent extraction of sinomenine and promote the internal aerobic bacteria to continuously produce sinomenine. Moreover, after standing for 3 to 8 days to reach the critical value of mildew, the interaction between microorganisms and Caulis Sinomenii and the transformation of internal substances all help to increase the extractability of sinomenine. Coupled with the synergistic effect of suitable temperature and other conditions during the drying process, the effective release and extraction of sinomenine are guaranteed from multiple links, and ultimately the extraction rate of sinomenine is significantly improved.
[0021] 2) The method of the present invention is relatively simple to operate. The harvested Caulis Sinomenii rhizomes are washed and cut into cylindrical segments, which are then squeezed, left to stand, sliced again, and dried. The steps are clear and easy to implement. Compared with the prior art, the present invention can improve the extraction rate of sinomenine at a lower cost, which helps to obtain more sinomenine in the production process, thereby improving production efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 The figure shows the effect of different compression ratios on the content of sinomenine, among which F01, F02 and F03 were compressed without treatment, F11, F12 and F13 were compressed to flatness, F21, F22 and F23 were compressed to 0.75 times, and F31, F32 and F33 were compressed to 0.5 times.
[0023] Figure 2 Figure 3 Effect of drying treatment at different temperatures on the content of sinomenine, where G11, G12 and G13 were dried at 60°C, G21, G22 and G23 were dried at 70°C, G31, G32 and G33 were dried at 80°C, G41, G42 and G43 were dried at 90°C, and G51, G52 and G53 were dried at 105°C. DETAILED DESCRIPTION
[0024] The technical solution of the present invention will be further described below in conjunction with specific embodiments of the present invention. It should be noted that the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments; these embodiments are only for a better understanding of the present invention, rather than limiting the scope of protection of the present invention.
[0025] Unless otherwise specified, all reagents and raw materials used in the present invention are commercially available products or products that can be prepared by known methods.
[0026] The Caulis Sinomenii roots used in the present invention are collected from the Qinling Mountains in Shaanxi Province.
[0027] Embodiment 1:
[0028] The present embodiment is a method for improving the extraction rate of sinomenine in Caulis Sinomenii. The underground stems of Caulis Sinomenii are harvested, washed, naturally dried, and cut into equal-length cylindrical segments of 10 cm in length. Pressure is then applied perpendicular to the axial direction to perform extrusion treatment. The sections are placed at 20°C for 7 days under well-ventilated indoor conditions to reach critical mildew. The sections are sliced again and dried at 90°C for 8 hours, and the moisture content is less than 13%.
[0029] The following study explored the effect of different compression degrees on the content of sinomenine. The cylindrical segments of Caulis Sinensis with equal lengths were compressed to 0.5 times, 0.75 times and completely flattened. The experiment was repeated 3 times for each compression multiple, which were respectively counted as F3 group, F2 group and F1 group. The group without compression treatment was counted as F0 group. The results of the effect of different compression degrees on the content of sinomenine are shown in Figure 1 The results of variance analysis are shown in Table 1.
[0030] Embodiment 2:
[0031] The present embodiment is a method for improving the extraction rate of sinomenine in Caulis Sinomenii. The roots of Caulis Sinomenii are picked, washed and naturally dried, and then cut into equal-length cylindrical segments with a length of 10 cm. Pressure is then applied perpendicular to the axial direction to perform an extrusion process so that the side thickness is shortened to 0.5 times of the original thickness. The roots are placed at 20°C for 7 days under well-ventilated indoor conditions to reach critical mildew, and then sliced again and dried for 8 hours, with a moisture content of less than 13%.
[0032] The following study explores the effect of drying at different temperatures on the content of sinomenine. The slices were cut into 2 mm thick slices, divided into 5 equal parts, and placed in a constant temperature drying oven at 60°C, 70°C, 80°C, 90°C, and 105°C for drying. The experiment was repeated 3 times at each drying temperature, and they were counted as Group G1, Group G2, Group G3, Group G4, and Group G5. The results of the effect of drying at different temperatures on the content of sinomenine are shown in Figure 2 The results of variance analysis are shown in Table 2.
[0033] Table 1 ANOVA results of different extrusion degree treatments
[0034] * p<0.05 ** p<0.01 Depend on Figure 1 As shown in Table 1, different types of extrusion treatments can improve the content of sinomenine compared with no treatment. The highest average sinomenine content was obtained in the F3 treatment (compressed to 0.5 times), which had a significant effect and was 61.2% higher than that in F0. After variance analysis, all the test results of samples with different crushing treatments showed significance (p<0.05), and the compression treatment showed significance at the 0.01 level for the test results (F=28.397, p=0.000). The comparison results were F1>F0; F2>F0; F3>F0; F3>F1; F3>F2.
[0035] Table 2 Results of variance analysis for drying treatments at different temperatures
[0036] * p<0.05 ** p<0.01 Depend on Figure 2 As shown in Table 2, the average contents of sinomenine after treatment at different temperatures are G4>G5>G3>G1>G2 in order; all the test results of samples with different drying treatments were significant (p<0.05), and the drying treatment was significant at the 0.01 level for the test results (F=19.026, p=0.000), and the comparison results were "105℃>60℃; 105℃>70℃; 80℃>60℃; 90℃>60℃; 80℃>70℃; 90℃>70℃; 90℃>80℃".
[0037] From the above data, we can see that extrusion treatment has a significant effect on the extraction rate of sinomenine. The extraction rate of samples subjected to extrusion treatment is generally higher, while the extraction rate of samples without extrusion or improper extrusion is lower. The drying temperature and time also have similar situations. A good extraction rate can be guaranteed within the appropriate range, and deviation will reduce the extraction rate.
[0038] This technology improves the extraction rate of sinomenine by multi-step processing of Caulis Sinomenii. The principle is to extrude Caulis Sinomenii cylindrical segments of a specific length, which can evenly destroy the tissue structure and promote aerobic bacteria to continuously produce sinomenine; static treatment can make Caulis Sinomenii reach the critical state of mildew, and the interaction between microorganisms and Caulis Sinomenii and the transformation of internal substances help to increase the extractability of sinomenine; the appropriate temperature and time during the drying process work synergistically to effectively remove moisture while ensuring the activity and stability of sinomenine. In summary, the beneficial effects of this technology are obvious. Through multi-step processing, the effective release and extraction of sinomenine are guaranteed from multiple links, and finally the extraction rate of sinomenine is significantly improved. Moreover, the method of the present invention is relatively simple to operate, the steps are clear and easy to implement, and the improvement of the extraction rate of sinomenine can be achieved at a lower cost, which helps to obtain more sinomenine in the production process, thereby improving production efficiency. The above embodiments are only for better explanation of the principles and practical applications of the present invention, so that those skilled in the relevant technical field can better understand and utilize the present invention, but they are not intended to limit the patent scope of the present invention. All equivalent changes made using the contents of the present invention are within the patent protection scope of the present invention.
Claims
1. A method for improving the extraction rate of sinomenine in Caulis Sinomenii, characterized in that: The harvested Caulis Australis underground stems are washed, cut into equal-length cylindrical segments I and squeezed, left to stand under indoor ventilation conditions, sliced again and dried; the squeezing process is to apply pressure perpendicular to the axial direction of the equal-length Caulis Australis cylindrical segments I, so that the side thickness is compressed to become cylindrical segments II.
2. The method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 1, characterized in that: The side thickness of the cylindrical section II is 0.4 to 0.8 times the side thickness of the cylindrical section I.
3. A method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 2, characterized in that: The length of the cylindrical section I is 6 to 14 cm.
4. The method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 1, characterized in that: The standing time is 3 to 8 days.
5. A method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 4, characterized in that: The standing temperature is 15-25°C.
6. The method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 1, characterized in that: The drying temperature is 70-100°C.
7. The method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 6, characterized in that: The drying time is 4 to 13 hours.
8. The method for improving the extraction rate of sinomenine in Caulis Sinomenii according to claim 7, characterized in that: The moisture content after drying is less than 13.0%.