Automatic cell culture workstation

Abstract

The invention relates to the technical field of cell culture, and discloses an automatic cell culture workstation, the automatic cell culture workstation comprises a transfer chamber, a consumable area, a culture area, an analysis area and an operation area, the transfer chamber is provided with a large mechanical arm; the consumable area is communicated with the transfer chamber and located on the first side of the transfer chamber; the culture area is communicated with the transfer chamber and is positioned on the second side of the transfer chamber; the analysis area is communicated with the transfer chamber and is positioned on the third side of the transfer chamber; the operation area is communicated with the transfer chamber and is positioned on the fourth side of the transfer chamber; wherein the large mechanical arm is used for transferring materials among the consumable cabin, the culture area, the analysis area and the operation area.

Description

Technical Field

[0001] The present application relates to the field of cell culture technology, for example, to an automated cell culture workstation. Background Art

[0002] Conventional cell culture is performed manually, which results in poor cell culture uniformity and easy contamination of samples.

[0003] In order to realize the automated culture of cells, an automatic cell culture workstation is disclosed in the related art, comprising a closed base shell, wherein a storage module, an operation module and a culture module are sequentially arranged inside the base shell, wherein: the storage module comprises a rotatable shelf and a first power source for driving the shelf to rotate; the operation module comprises a mechanical clamp for clamping culture bottles or reagent tubes, a storage box for storing reagent tubes, a centrifugal module for centrifuging reagent tubes, a capping module for capping culture bottles or reagent tubes, a transfer module and a liquid adding module; the culture module comprises a sealing chamber, a culture rack located in the sealing chamber and a second power source for driving the culture rack to rotate, the culture rack is used to store culture bottles, and the side of the sealing chamber is provided with a transfer window for the mechanical clamp to pass through and a side sealing door for closing the transfer window.

[0004] In the process of implementing the embodiments of the present disclosure, it is found that there are at least the following problems in the related art:

[0005] The automated cell culture workstation of the related art arranges storage modules, operation modules and culture modules in the form of an assembly line, and the cell culture is carried out in a manner similar to an assembly line, which makes it difficult to meet the differentiated culture and observation requirements of different cells.

[0006] It should be noted that the information disclosed in the above background technology section is only used to enhance the understanding of the background of the present application, and therefore may include information that does not constitute the prior art known to ordinary technicians in the field. Summary of the invention

[0007] In order to provide a basic understanding of some aspects of the disclosed embodiments, a brief summary is given below. The summary is not an extensive review, nor is it intended to identify key / critical components or delineate the scope of protection of these embodiments, but rather serves as a prelude to the detailed description that follows.

[0008] The disclosed embodiment provides an automated cell culture workstation, so that the automated cell culture workstation can organize different cell culture processes more flexibly.

[0009] In some embodiments, an automated cell culture workstation includes a transfer chamber, a consumables area, a culture area, an analysis area and an operation area, wherein the transfer chamber is provided with a large robotic arm; the consumables area is connected to the transfer chamber and located on the first side of the transfer chamber; the culture area is connected to the transfer chamber and located on the second side of the transfer chamber; the analysis area is connected to the transfer chamber and located on the third side of the transfer chamber; the operation area is connected to the transfer chamber and located on the fourth side of the transfer chamber; wherein the large robotic arm is used to transfer materials between the consumables compartment, the culture area, the analysis area and the operation area.

[0010] In some embodiments, the first side is opposite to the second side, and the third side is opposite to the fourth side.

[0011] In some embodiments, the culture area includes a transfer cabin and multiple culture cabins, wherein one end of the transfer cabin is connected to the transfer chamber; multiple culture cabins are arranged on one side or both sides of the transfer cabin, and the culture cabin is provided with an automatic door, and when the automatic door is opened, the corresponding culture cabin is connected to the transfer cabin.

[0012] In some embodiments, the culture area further comprises an automatic slide and a transfer cart, wherein the automatic slide is arranged on the transfer cabin, and the automatic slide covers the automatic doors of the multiple culture cabins; the transfer cart is slidably arranged on the automatic slide.

[0013] In some embodiments, the culture area also includes an incubator turntable rack and an incubator pick-and-place device, wherein the incubator turntable rack is arranged in the culture cabin; the incubator pick-and-place device is used to transfer culture bottles between the transfer cart and the incubator turntable rack.

[0014] In some embodiments, the consumable area includes a loading chamber and a transfer chamber, wherein the loading chamber is connected to the transfer chamber, and the loading chamber is used to temporarily store materials; the transfer chamber is connected to the loading chamber, and the transfer chamber is provided with a hatch connecting to the external environment.

[0015] In some embodiments, a glove compartment is provided on the outward side of the loading chamber.

[0016] In some embodiments, a glove compartment is provided on the outward side of the operating area.

[0017] In some embodiments, a glove compartment is disposed on the outward side of the analysis area.

[0018] In some embodiments, the consumables area further includes a material slide rail, the travel of the material slide rail covers the loading chamber and the transfer chamber, and the material slide rail is used to transfer materials between the loading chamber and the transfer chamber.

[0019] In some embodiments, the consumable area also includes a consumable turntable and a picking and placing device, wherein the consumable turntable rack is arranged in the loading chamber; and the picking and placing device is used to transfer consumables between the consumable turntable rack and the transfer chamber.

[0020] In some embodiments, the automated cell culture workstation further comprises an automatic counting device, which is disposed in the analysis area.

[0021] In some embodiments, the automated cell culture workstation further comprises a centrifuge disposed in the transfer chamber.

[0022] In some embodiments, the automated cell culture workstation further includes a liquid adding module, and the liquid adding module is disposed in the operating area.

[0023] The automated cell culture workstation provided by the embodiments of the present disclosure can achieve the following technical effects:

[0024] The transfer chamber is connected with the consumables area, culture area, analysis area and operation area, realizing independent operation and collaborative work of each functional area. This design not only improves the flexibility of the workstation, but also allows personalized configuration according to the needs of different cell cultures. By setting up a large robotic arm in the transfer chamber, the function of automatically transferring materials between the consumables area, culture area, analysis area and operation area is realized. This automated material transfer method improves the efficiency and accuracy of the operation, and can meet the cell culture needs under different processes.

[0025] The above general description and the following description are exemplary and explanatory only and are not intended to limit the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] One or more embodiments are exemplarily described by corresponding drawings, which do not limit the embodiments. Elements with the same reference numerals in the drawings are shown as similar elements, and the drawings do not constitute a scale limitation, and wherein:

[0027] Figure 1 is a schematic structural diagram of an automated cell culture workstation provided by an embodiment of the present disclosure;

[0028] Figure 2 is a schematic structural diagram of another automated cell culture workstation provided by an embodiment of the present disclosure;

[0029] Figure 3 is a schematic structural diagram of another automated cell culture workstation provided by an embodiment of the present disclosure;

[0030] Figure 4The embodiment of the present disclosure provides a structural schematic diagram of another automated cell culture workstation provided by the embodiment of the present disclosure;

[0031] Figure 5 is a schematic structural diagram of a working cabin for an automated cell culture workstation provided by an embodiment of the present disclosure;

[0032] Figure 6 is a schematic cross-sectional view of a working cabin for an automated cell culture workstation provided by an embodiment of the present disclosure;

[0033] Figure 7 is a schematic structural diagram of an automatic counting device for an automated cell culture workstation provided by an embodiment of the present disclosure;

[0034] Figure 8 yes Figure 7 The enlarged schematic diagram of the middle A part;

[0035] Fig. 9 is a schematic structural diagram of another automatic counting device for an automated cell culture workstation provided by an embodiment of the present disclosure;

[0036] Fig.10 yes Figure 2 The enlarged schematic diagram of the middle part B;

[0037] Fig.11 yes Figure 2 The enlarged schematic diagram of the middle C part;

[0038] Fig.12 It is a structural schematic diagram of a liquid adding device for an automated cell culture workstation provided in an embodiment of the present disclosure.

[0039] Reference numerals:

[0040] 100: working cabin; 111: first partition; 112: second partition; 121: purification air duct; 122: first high-efficiency filter; 123: purification fan; 130: disinfection pipeline; 141: exhaust air duct; 142: exhaust fan; 143: second high-efficiency filter; 144: air inlet; 145: exhaust outlet; 146: connecting port; 150: flow equalization membrane; 200: counting device; 210: gun head rack; 220: counting plate rack; 230: centrifuge tube rack; 240: cell counter; 250: pipette; 251: quick plug connector; 252: gun head release device; 253: clamp; 261: travel track; 262: travel beam; 263: lifting track; 264: clamp arm; 271: cryobox rack; 281: cover opening device; 282: glove compartment ;290: conveyor belt;310: transfer chamber;320: consumables area;321: loading chamber;322: transfer chamber;323: material slide rail;324: consumables turntable rack;325: pick-and-place device;330: culture area;331: transfer cabin;332: culture cabin;335: automatic slide rail;336: transfer trolley;340: analysis area;350: operation area;360: large robotic arm;400: liquid adding device;411: insulation shell;412: first temperature control module;413: liquid container;421: first bottle rack;430: liquid adding head;440: peristaltic pump;450: weighing module;460: second temperature control module;470: second bottle rack;480: transfer device;500: bottom plate;510: centrifuge;530: microscope;540: resuscitation instrument. DETAILED DESCRIPTION

[0041] In order to be able to understand the features and technical contents of the embodiments of the present disclosure in more detail, the implementation of the embodiments of the present disclosure is described in detail below in conjunction with the accompanying drawings. The attached drawings are for reference only and are not used to limit the embodiments of the present disclosure. In the following technical description, for the convenience of explanation, a full understanding of the disclosed embodiments is provided through multiple details. However, one or more embodiments can still be implemented without these details. In other cases, to simplify the drawings, well-known structures and devices can be simplified for display.

[0042] The terms "first", "second", etc. in the specification and claims of the embodiments of the present disclosure and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the terms used in this way can be interchanged where appropriate, so that the embodiments of the embodiments of the present disclosure described herein. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions.

[0043] In the embodiments of the present disclosure, the terms "upper", "lower", "inside", "middle", "outside", "front", "back" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. These terms are mainly intended to better describe the embodiments of the present disclosure and their embodiments, and are not intended to limit the indicated devices, elements or components to have a specific direction, or to be constructed and operated in a specific direction. Moreover, in addition to being used to indicate directions or positional relationships, some of the above terms may also be used to indicate other meanings. For example, the term "upper" may also be used to indicate a certain dependency or connection relationship in certain circumstances. For those of ordinary skill in the art, the specific meanings of these terms in the embodiments of the present disclosure can be understood according to specific circumstances.

[0044] In addition, the terms "disposed", "connected", and "fixed" should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral structure; it can be a mechanical connection, or an electrical connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be an internal connection between two devices, elements, or components. For those of ordinary skill in the art, the specific meanings of the above terms in the embodiments of the present disclosure can be understood according to specific circumstances.

[0045] Unless otherwise stated, the term "plurality" means two or more.

[0046] In the embodiment of the present disclosure, the character " / " indicates that the preceding and following objects are in an "or" relationship. For example, A / B indicates: A or B.

[0047] The term "and / or" is a description of the association relationship between objects, indicating that three relationships can exist. For example, A and / or B means: A or B, or, A and B.

[0048] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present disclosure may be combined with each other.

[0049] Generally, cell culture is carried out manually, which has the problems of poor cell culture uniformity and easy contamination of samples. In order to realize the automated culture of cells, an automatic cell culture workstation is disclosed in the related art, including a closed base shell, and a storage module, an operation module and a culture module are arranged in sequence inside the base shell, wherein: the storage module includes a rotatable shelf and a first power source for driving the shelf to rotate; the operation module includes a mechanical clamp for clamping a culture bottle or a reagent tube, a storage box for storing the reagent tube, a centrifugal module for centrifuging the reagent tube, a capping module for screwing the cap on the culture bottle or the reagent tube, a transfer module and a liquid adding module; the culture module includes a sealed chamber, a culture rack located in the sealed chamber and a second power source for driving the culture rack to rotate, the culture rack is used to store the culture bottle, and the side of the sealed chamber is provided with a transfer window for the mechanical clamp to pass through and a side sealing door for closing the transfer window. The problem with the related technology is that the automated cell culture workstation arranges the storage module, operation module and culture module in the form of an assembly line, and the cell culture is carried out in a manner similar to an assembly line, which makes it difficult to meet the differentiated culture and observation needs of different cells.

[0050] In order to make the automated cell culture workstation more flexible in organizing different cell culture processes, Figure 1-12 As shown, an embodiment of the present disclosure provides an automated cell culture workstation, which includes a transfer chamber 310, a consumable area 320, a culture area 330, an analysis area 340 and an operation area 350, wherein the transfer chamber 310 is provided with a large robotic arm 360; the consumable area 320 is connected to the transfer chamber 310 and is located on a first side of the transfer chamber 310; the culture area 330 is connected to the transfer chamber 310 and is located on a second side of the transfer chamber 310; the analysis area 340 is connected to the transfer chamber 310 and is located on a third side of the transfer chamber 310; the operation area 350 is connected to the transfer chamber 310 and is located on a fourth side of the transfer chamber 310; wherein the large robotic arm 360 is used to transfer materials between the consumable compartment, the culture area 330, the analysis area 340 and the operation area 350.

[0051] The automated cell culture workstation provided in the embodiment of the present disclosure is centered on the transfer chamber 310. Around the transfer chamber 310, there are arranged a consumables area 320, an analysis area 340, a culture area 330 and an operation area 350.

[0052] The operating room is provided with a large mechanical arm 360, which is used to grab materials and transfer them over a long span between the consumable area 320, the analysis area 340, the operating room and the culture. The materials include but are not limited to culture bottles, centrifuge bottles, centrifuge tubes and reagent bottles of different specifications.

[0053] Since the transfer chamber 310 is connected to the consumables area 320, the analysis area 340, the culture area 330 and the operation area 350 at the same time, the mechanical arm of the transfer chamber 310 can quickly realize the upstream and downstream coordination of materials between any two functional areas. For example, when storing cells, the large mechanical arm 360 can grab the culture bottle from the consumables area 320 and transfer it to the culture area 330; when taking out cells, the large mechanical arm 360 can grab the culture bottle from the culture area 330 and transfer it to the consumables area 320; when replenishing consumables, the large mechanical arm 360 can grab the consumables from the consumables area 320 and transfer them to the analysis area 340 or the operation area 350; when observing cultured cells, the large mechanical arm 360 can grab the culture bottle from the culture area 330 and transfer it to the analysis area 340.

[0054] The transfer chamber 310 is connected with the consumable area 320, the culture area 330, the analysis area 340 and the operation area 350, so that the independent operation and coordinated work of each functional area can be realized. This design not only improves the flexibility of the workstation, but also allows personalized configuration according to the needs of different cell cultures. By setting a large robotic arm 360 in the transfer chamber 310, the function of automatically transferring materials between the consumable area 320, the culture area 330, the analysis area 340 and the operation area 350 is realized. This automated material transfer method improves the efficiency and accuracy of the operation, and can meet the cell culture needs under different processes.

[0055] Optionally, the first side is opposite to the second side, and the third side is opposite to the fourth side.

[0056] During the cell culture process, when taking out cells, it is usually necessary to analyze or operate the cells, and when storing cells, it is also usually necessary to analyze and operate the cells. Therefore, the point-to-point transmission frequency between the consumable area 320 and the culture area 330 is relatively low. However, there is more transmission between the consumable area 320 and the operation area 350 and the analysis area 340, and the culture area 330 is also used for more transmission between the operation area 350 and the analysis area 340. When the first side is opposite to the second side and the third side is opposite to the fourth side, the transmission distance between the consumable area 320 and the operation area 350 and the analysis area 340 is relatively short, and the distance between the culture area 330 and the operation area 350 and the analysis area 340 is also relatively short. With such an arrangement, the situation of large-scale material dispatching by the large robotic arm 360 can be reduced as a whole, thereby improving the working efficiency of the automated cell culture workstation.

[0057] Optionally, the culture area 330 includes a transfer cabin 331 and multiple culture cabins 332, wherein one end of the transfer cabin 331 is connected to the transfer chamber 310; multiple culture cabins 332 are arranged on one side or both sides of the transfer cabin 331, and the culture cabin 332 is provided with an automatic door. When the automatic door is opened, the corresponding culture cabin 332 is connected to the transfer cabin 331.

[0058] The transfer chamber 331 of the culture area 330 is used to dock with the transfer chamber 310. Multiple culture chambers 332 are arranged on both sides of the transfer chamber 331, which can improve the storage capacity of the automated cell culture workstation, thereby improving the overall culture efficiency of the automated cell culture workstation. In addition, multiple culture chambers 332 are provided, and different culture environments can be set for multiple cultures, which is conducive to the automated cell culture workstation to simultaneously culture and store multiple different cells.

[0059] Optionally, the culture area 330 also includes an automatic slide 335 and a transfer cart 336 , wherein the automatic slide 335 is arranged on the transfer cabin 331 , and the automatic slide 335 covers the automatic doors of multiple culture cabins 332 ; the transfer cart 336 is slidably arranged on the automatic slide 335 .

[0060] The combination of the automatic slide rail 335 and the transfer trolley 336 can distribute the culture bottles to each culture chamber 332 quickly and efficiently.

[0061] Optionally, the culture area 330 also includes an incubator turntable rack and an incubator pick-up and placement device 325, wherein the incubator turntable rack is arranged in the culture chamber 332; the incubator pick-up and placement device 325 is used to transfer culture bottles between the transfer cart 336 and the incubator turntable rack.

[0062] The incubator turntable rack is provided so that the incubator cabin 332 can store more culture bottles in a three-dimensional manner. In addition, the incubator turntable rack is in a rotatable form, which facilitates the incubator fetching and placing device 325 to locate the storage position, thereby accurately taking out or storing the culture bottle from the incubator turntable rack.

[0063] Optionally, the consumable area 320 includes a loading chamber 321 and a transfer chamber 322, wherein the loading chamber 321 is connected to the transfer chamber 310, and is used for temporarily storing materials; the transfer chamber 322 is connected to the loading chamber 321, and is provided with a hatch connected to the external environment.

[0064] When the door of the transfer chamber 322 is opened, it will be connected to the outside environment. The transfer chamber 322 is not directly connected to the transfer chamber 310, but the material transfer is realized through the loading chamber 321, which can improve the cleanliness inside the automated culture workstation. The loading chamber 321 not only isolates the external environment, but also serves as a temporary storage of materials. In this way, the ability of the automated cell culture workstation to store consumables can be improved, thereby further improving the culture efficiency of the automated cell culture workstation.

[0065] Optionally, a glove compartment 282 is provided on the outward side of the loading chamber 321 .

[0066] For some packaged materials, they can enter the transfer chamber 322 with the package. The packaged materials entering the transfer chamber 322 enter the loading chamber 321 and then open the package, which can reduce the pollution of the materials by the external environment. The glove compartment 282 is set on the outer side of the loading chamber 321. The user can manually remove the package and sort the materials through the glove compartment 282, which improves the material adaptability and fault tolerance of the automated cell culture workstation.

[0067] Optionally, a glove compartment 282 is provided on the outward side of the operating area 350 .

[0068] For some special processes, or when the automated cell culture workstation requires manual intervention, the user can timely intervene in the cell culture through the glove compartment 282 of the operation area 350. This can improve the fault tolerance of the automated cell culture workstation.

[0069] Optionally, a glove compartment 282 is provided on the outward side of the analysis area 340 .

[0070] For some special processes, or when the automated cell culture workstation requires manual intervention, the user can intervene in the cell culture in time through the glove compartment 282 of the analysis area 340. This can improve the fault tolerance of the automated cell culture workstation.

[0071] Optionally, the consumable area 320 further includes a material slide 323 , the travel of the material slide 323 covers the loading chamber 321 and the transfer chamber 322 , and the material slide 323 is used to transfer materials between the loading chamber 321 and the transfer chamber 322 .

[0072] The transfer of materials from the transfer chamber 321 to the loading chamber 321 is completed through the material slide rail 323, so that the automated cell culture workstation can realize automatic loading.

[0073] Optionally, the loading chamber 321 further includes a consumable turntable and a pick-and-place device 325 , wherein the consumable turntable rack 324 is disposed in the loading chamber 321 ; and the pick-and-place device 325 is used to transfer consumables between the consumable turntable rack 324 and the transfer chamber 310 .

[0074] The consumable turntable rack 324 can be stacked to store more consumables. The form of the turntable rack is conducive to the accurate positioning of the designated consumables by the pick-and-place device 325 during loading and pick-and-place.

[0075] Optionally, the automated cell culture workstation further includes an automatic counting device 200 , which is disposed in the analysis area 340 .

[0076] The automatic counting device 200 is provided to facilitate the automated cell culture workstation to understand the cell culture progress and culture effect and to adjust the cell culture program in time.

[0077] Optionally, the automatic counting device 200 includes a gun tip rack 210, a counting rack, a centrifuge tube rack 230, a cell counter 240 and a pipette 250, wherein the gun tip rack 210 is used to place multiple gun tips; the counting plate rack 220 is used to place a cell counting plate; the centrifuge tube rack 230 is used to place a centrifuge tube; the cell counter 240 is provided with a cell counting area; the pipette 250 is provided with a clamp 253 and a pipette, the pipette has a quick-connect connector 251 and a gun tip release device 252, when the pipette moves toward the gun tip, the gun tip is connected through the quick-connect connector 251, and the gun tip release device 252 releases the gun tip when it is activated, the pipette is used to install the gun tip from the gun tip rack 210 and absorb the cell fluid from the centrifuge tube into the cell counting plate, and the clamp 253 is used to move the cell counting plate from the counting plate rack 220 to the cell counting area of ​​the cell counter 240.

[0078] In the embodiment of the present disclosure, the automatic counting device 200 includes a consumable material storage part, a moving part and a detection part.

[0079] The consumable storage part includes a gun tip rack 210, a counting rack plate and a centrifuge tube rack 230. The gun tip rack 210 is used to place a pipette tip. The counting rack plate is used to place a cell counting plate. The centrifuge tube rack 230 is used to place one or more centrifuge tubes.

[0080] The detection part includes a cell counter 240. The cell counter 240 includes a detection area, and the detection area is used to place the cell counting plate after adding the cell solution.

[0081] The mobile part includes a pipette 250. The pipette 250 is provided with a clamp 253 and a pipette gun. The pipette gun is provided with a quick plug connector 251 and a gun tip release device 252.

[0082] Taking a use process as an example, the pipette moves to the top of the gun tip rack 210, and the pipette moves toward the gun tip rack 210, and the gun tip is fixed by the quick plug connector 251. The pipette with the gun tip moves to the centrifuge tube rack 230 to absorb the cell fluid from the centrifuge tube. After absorbing the cell fluid, the pipette moves to the counting plate rack 220 to add cell fluid to the cell counting plate. After adding the cell fluid, the pipette moves to the discard position, and the gun tip release device 252 is activated to discard the gun tip. The clamp 253 moves to the counting plate rack 220, and moves the cell counting plate after the cell fluid is added to the detection area of ​​the cell counter 240. The cell counter 240 counts the cells and feeds back the counting results.

[0083] The automatic counting device 200 provided in the embodiment of the present disclosure clamps the cell counting plate through the clamp 253, and the clamping process is smooth and not easy to fall. The pipette has a quick plug connector 251 and a gun head release device 252, and the pipette can automatically replace the gun head, thereby improving the automation of the automatic counting device 200.

[0084] Optionally, the pipette 250 also includes a traveling track 261, a traveling beam 262, a lifting track 263 and a clamping arm 264, wherein the traveling track 261 includes a first track and a second track arranged in parallel, and the first track and the second track are arranged overhead; the traveling beam 262 is slidably arranged on the first track and the second track; the lifting track 263 is connected to the traveling beam 262; the clamping arm 264 is slidably arranged on the lifting track 263, and the clamp 253 and the pipette gun are connected to the clamping arm 264; wherein, when the traveling beam 262 slides along the first track and the second track, the clamping arm 264 translates along the first direction, and when the lifting track 263 slides along the traveling beam 262, the clamping arm 264 translates along the second direction.

[0085] In order to realize the movement of the clamp 253 and the pipette, the pipette 250 also includes a motion device. The motion device includes a travel track 261, a travel beam 262, a lifting track 263 and a clamp arm 264. The clamp arm 264 is used as the execution end of the pipette 250 to connect the clamp 253 and the pipette.

[0086] The traveling track 261 includes a first track and a second track. The first track and the second track are higher than the plane where the cell counter 240 is located, so that a shielding frame is provided for the automatic counting device 200, which can play a certain protective role on the cell counter 240 and the consumables rack. The two ends of the traveling beam 262 are respectively connected to the first track and the second track, and can slide along the length direction of the traveling track 261. When the clamping arm 264 is connected to the traveling beam 262, the movement in the first direction of the horizontal plane is realized by sliding the traveling beam 262 on the traveling track 261. The lifting track 263 is slidably arranged on the traveling beam 262, and can slide along the length direction of the traveling beam 262. When the lifting track 263 slides along the traveling beam 262, the clamping arm 264 connected to the lifting track 263 realizes the movement in the second direction of the horizontal plane. The lifting track 263 is vertically arranged, and the clamping arm 264 is slidably arranged on the lifting track 263. When the clamp arm 264 moves up and down along the lifting track 263 , it drives the clamp 253 and the pipette gun to move in the height direction.

[0087] By such a configuration, the three-dimensional movement of the fixture 253 and the liquid transfer gun can be achieved at a relatively low cost. In addition, the frame structure composed of the traveling track 261 and the traveling beam 262 can provide a certain degree of protection for the cell counter 240 .

[0088] Optionally, the cell counter 240, the gun tip rack 210, and the counting plate rack 220 are located within the first track and the second track.

[0089] The cell counter 240, the gun tip rack 210, and the counting plate rack 220 are located within the first track and the second track, which means that the first track and the second track are within the projection range of the plane where the cell counter 240 is located. This is conducive to the replacement of the gun tip of the pipette 250, and is also conducive to the transfer of the cell counting plate and cell fluid by the pipette 250.

[0090] Optionally, the clamp 253 is also used to clamp the gun head holder plate 210 .

[0091] After the gun tips are used up, the fixture 253 can transfer the empty gun tip rack 210 to another position, and transfer the new gun tip rack 210 with gun tips to the gun tip replacement position. With such a setting form, the automatic counting device 200 can realize the automatic replacement of the gun tip rack 210, further improving the automation degree of the automatic counting device 200.

[0092] Optionally, the automatic counting device 200 is configured with a first positioning structure.

[0093] As an implementation method, the bottom plate 500 of the automatic counting device 200 is recessed downward to form a first positioning pit, and the first positioning pit is used as a first positioning structure. The gun head support plate 210 is placed in the first positioning pit.

[0094] Such a configuration is adopted to facilitate the mobile positioning of the gun head frame 210, and facilitate the more accurate replacement of the gun head by the pipette 250. In addition, the form of the positioning pit can also reduce the occupied space of the gun head frame 210, and increase the movable range of the pipette 250.

[0095] Optionally, the automatic counting device 200 further includes a weighing device, and the weighing device is used to obtain the weight of the gun head rack 210 .

[0096] In this way, the automatic counting device 200 can not only detect whether the tip rack 210 is loaded, but also further obtain the number of remaining tips through the weighing value of the weighing device. Since the pipette picks up the tips in a certain order, the number of remaining tips can provide accurate position information for the next time the pipette 250 picks up the tips.

[0097] Optionally, the clamp 253 is also used to clamp the counting plate rack 220 .

[0098] After the cell counting plate is used up, the clamp 253 can transfer the counting plate rack 220 to another position, and move the new counting plate rack 220 with the cell counting plate to the liquid adding position. With such a setting form, the automatic counting device 200 can realize the automatic loading of the cell counting plate, further improving the automation degree of the automatic counting device 200.

[0099] Optionally, the automatic counting device 200 is configured with a second positioning structure.

[0100] As an implementation method, the bottom plate 500 of the automatic counting device 200 is recessed downward to form a second positioning pit, and the second positioning pit is used as a second positioning structure. The gun head support plate 210 is placed in the second positioning pit.

[0101] Such a configuration is beneficial to the mobile positioning of the counting plate rack 220, the accurate addition of cell fluid to the cell counting plate by the pipette gun, and the accurate movement of the cell counting plate by the fixture 253. In addition, the positioning pit can also reduce the occupied space of the counting plate rack 220 and increase the movable range of the pipette 250.

[0102] Optionally, the centrifuge tube rack 230 is provided with a photoelectric tube, and the photoelectric tube is used to detect whether a centrifuge tube is placed in the centrifuge tube rack 230 .

[0103] Such a configuration is conducive to the pipette 250 to absorb the cell fluid from the centrifuge tube.

[0104] Optionally, the automatic counting device 200 also includes a freezing box rack 271 and a freezing box, wherein the freezing box rack 271 is used to place the freezing box, and the freezing box rack 271 is located within the first track and the second track; the freezing box is placed on the freezing box rack 271; wherein the pipette 250 also uses the gun tip to pack the cell fluid into the freezing tube in the freezing box, and the clamp 253 is also used to clamp the freezing box.

[0105] After the cultured cells meet the culture requirements, the cell fluid can be dispensed into the cryopreservation tubes of the cryopreservation box by the pipette 250. When dispensing the cell fluid, the pipette 250 replaces the gun head, absorbs the cell fluid or functional liquid and injects it into the cryopreservation tube. The transfer of the cryopreservation box is still carried out first by the clamp 253. In this way, the pipette 250 of the automatic counting device 200 can have more functions.

[0106] Optionally, the automatic counting device 200 further includes a cover opening device 281, which is used to open and close the box cover of the freezing box and / or the tube cover of the freezing tube, and the clamp 253 is also used to transfer the freezing box between the cover opening device 281 and the freezing box rack 271.

[0107] A cover opening device 281 is provided to realize automatic packaging of cell fluid. Taking one usage as an example, the clamp 253 transfers the cryopreservation box to the cover opening device 281. The cover opening device 281 opens the box lid of the cryopreservation box and opens the can lid of the cryopreservation tube, and the clamp 253 moves the cryopreservation box to the sub-packaging position. The pipette 250 packs the cell fluid into the cryopreservation tube. After the sub-packaging is completed, the clamp 253 moves the cryopreservation box to the cover opening device 281 for closing the lid. After the lid is closed, the clamp 253 transfers the cryopreservation box to the next station.

[0108] Optionally, the cell counter 240 can switch between a bright field counting mode and a fluorescence counting mode.

[0109] This allows you to quickly switch between brightfield and fluorescence counting according to your experimental needs without having to change equipment or perform complex settings. The automated switching of counting modes reduces experimental time and improves work efficiency.

[0110] The embodiment of the present disclosure provides an automated cell culture workstation, which includes an operation room and the above-mentioned counting device 200 , wherein the automatic counting device 200 is arranged in the operation room.

[0111] By using the automated cell culture workstation provided by the embodiment of the present disclosure, the automatic counting device 200 can automatically replace the gun tip, with a high degree of automation and high culture efficiency.

[0112] Optionally, the automated cell culture workstation further includes a transfer chamber 310 and a large robotic arm 360, wherein the transfer chamber 310 is connected to the operation chamber; the large robotic arm 360 is disposed in the transfer chamber 310, and the large robotic arm 360 is used to transfer cryopreservation boxes and centrifuge tubes between the transfer chamber 310 and the operation chamber.

[0113] A large robotic arm 360 is provided to realize a large-scale transfer of materials such as cell fluid, functional liquid and gun tip rack 210, further improving the automation level of the automated cell culture workstation.

[0114] Optionally, the large robotic arm 360 is located on one side of the cell counter 240 , and the operating room is located on the other side of the cell counter 240 and is provided with a glove compartment 282 ; the counting device 200 also includes: a conveyor belt 290 for conveying the freezing box to a side close to the glove compartment 282 .

[0115] The glove compartment 282 is a window for setting flexible gloves. The user can operate the cell solution in the operation through the glove compartment 282 under a biological isolation. In this way, the user can manually intervene in all cases, which improves the fault tolerance of the automated cell culture workstation.

[0116] A conveyor belt 290 is provided to transfer materials such as freezing boxes located in the transfer room 310 or the operation room to the operation area 350 of the glove compartment 282, further facilitating use by users.

[0117] Optionally, the automated cell culture workstation further includes a centrifuge 510 , which is disposed in the transfer chamber 310 .

[0118] A centrifuge 510 is provided to facilitate the automated cell culture workstation to perform centrifugal analysis on the lower package.

[0119] Optionally, the automated cell culture workstation further includes a liquid adding module, which is disposed in the operation area 350 .

[0120] A liquid adding module is provided, which is beneficial for the automated cell culture workstation to add functional liquids to centrifuge tubes and reagent bottles.

[0121] Optionally, the cell culture workstation includes an operation cabin, and the liquid adding device 400 includes a heat preservation shell 411, a first temperature control module 412, a liquid container 413, a first bottle rack 421, a liquid adding head 430 and a peristaltic pump 440, wherein the heat preservation shell 411 is arranged outside the operation cabin, and the heat preservation shell 411 is configured with a liquid adding space; the first temperature control module 412 is used to control the temperature of the liquid adding space at a first temperature; the liquid container 413 is arranged in the liquid adding space, and the liquid container 413 Used to store functional liquid; a first bottle rack 421, which is arranged inside the operation cabin, and the first bottle rack 421 is used to place liquid bottles; a liquid adding head 430, which is movably arranged corresponding to the first bottle rack 421, and the liquid bottle is located below the liquid adding head 430 when the liquid adding head 430 is located at a first relative position, and the liquid adding head 430 avoids the liquid bottle when the liquid adding head 430 is located at a second relative position; a peristaltic pump 440, the liquid inlet end is connected to the liquid bottle through a liquid inlet pipe, and the liquid outlet end extends to the liquid adding head 430 through a liquid outlet pipe.

[0122] The cell culture workstation has an operation cabin, which is isolated from the external environment, thus improving the cleanliness of the interior of the operation cabin.

[0123] The liquid adding device 400 includes a heat-insulating housing 411 disposed outside the operation cabin, and the heat-insulating housing 411 is used to store a liquid container 413. The heat-insulating housing 411 is located outside, which is convenient for the user to add liquid to the liquid storage container or replace different functional liquids. Since the liquid adding module is located outside the operation cabin, even if the volume is large, it will not affect the internal space of the operation cabin. This allows the liquid storage container to store more functional liquids, thereby improving the ability of the automated cell culture workstation to work continuously.

[0124] In order to reduce or avoid the functional liquid from becoming ineffective due to high temperature, a first temperature control module 412 is provided inside the heat-insulating shell 411. The first temperature control module 412 cooperates with the heat-insulating shell 411 to keep the temperature of the liquid adding space at a relatively low temperature, which is conducive to the storage of the functional liquid.

[0125] Inside the operating cabin, a first bottle rack 421 is provided, and the first bottle rack 421 is used to place one or more liquid bottles. Compared with the liquid storage container, the liquid bottle stores less functional liquid to meet the use requirements of the automated cell culture workstation for a period of time. This can reduce the waste of functional liquid. The liquid adding head 430 and the liquid storage container are connected by a peristaltic pump 440, a liquid inlet pipe and a liquid outlet pipe. When the peristaltic pump 440 is running, the functional liquid in the liquid storage container is pumped to the liquid adding head 430, thereby filling the liquid bottle below the liquid adding head 430.

[0126] The liquid adding head 430 is in a movable form. When the liquid adding head 430 moves to the first relative position, it is located directly above the liquid bottle, and at this time, the liquid bottle can receive the functional liquid flowing out of the liquid adding head 430. When the liquid adding head 430 moves to the second relative position, it avoids the liquid bottle, so that the liquid bottle can overflow from the first bottle rack 421. With such a setting, the liquid bottle and the liquid storage container are no longer in a one-to-one correspondence, but can be used as a consumable material for a single use. This can reduce or avoid the contamination of the functional liquid in the liquid bottle. Since the liquid bottle can be taken out from the first bottle rack 421, the liquid adding position and the use position of the liquid bottle can be different positions. For example, the liquid adding position is close to the edge of the operating cabin to reduce interference with other moving parts, and the use position is located in the middle of the working cabin 100 to reduce the scheduling stroke.

[0127] When using the liquid adding device 400 provided in the embodiment of the present disclosure, the heat-insulating shell 411 is located outside the operating cabin, so that more functional liquids can be stored without occupying the space of the operating cabin; a first temperature control module 412 is provided in the heat-insulating shell 411, which can cooperate with the heat-insulating shell 411 to maintain a relatively low temperature environment, thereby extending the shelf life of the functional liquid; the movable liquid adding head 430 cooperates with the first bottle rack 421 and the liquid bottle. The liquid bottle, as a consumable, reduces the situation where the functional liquid fails due to contamination. The movable liquid adding head 430 allows the liquid bottle to be easily moved, which facilitates the scheduling of functional liquids in the automated cell culture workstation; the liquid adding method in which the liquid adding head 430 does not contact the liquid bottle avoids the backflow of the functional liquid, and the functional liquid in the liquid container 413 is not easily contaminated.

[0128] Optionally, the liquid filling head 430 can be flipped.

[0129] The liquid adding head 430 is in a flipping form, and the space required for movement is small.

[0130] Optionally, the first bottle rack 421 is movable, or the liquid adding head 430 is movable.

[0131] Any movement between the first bottle rack 421 and the liquid adding head 430 can switch the first relative position and the second relative position of the liquid adding head 430, so that the liquid adding head 430 switches to add liquid to the liquid bottle and avoid the liquid bottle.

[0132] Optionally, the first temperature is greater than or equal to 2 degrees Celsius and less than or equal to 8 degrees Celsius.

[0133] Within this temperature range, functional liquids can be stored for longer periods of time and are less likely to become ineffective.

[0134] Optionally, the first temperature control module 412 includes a semiconductor refrigeration plate, wherein a cooling end of the semiconductor refrigeration plate faces the inside of the liquid storage space and a heating end faces the outside of the liquid storage space.

[0135] The semiconductor refrigeration chip is small in size and low in cost, so that the liquid adding device 400 can have a smaller size and lower cost.

[0136] Optionally, the liquid inlet pipe and / or the liquid outlet pipe are in heat transfer contact with the heating end of the semiconductor refrigeration plate.

[0137] The functional liquid needs a certain temperature when used. Such an arrangement can be used to heat the functional liquid that has entered the liquid bottle.

[0138] Optionally, the liquid adding module further includes a control unit, which is configured to increase the power or start-up rate of the semiconductor refrigeration plate when the peristaltic pump 440 is started.

[0139] When maintaining the low temperature environment of the liquid storage space, the semiconductor refrigeration chip only needs to operate at a lower power or start-up rate. When the peristaltic pump 440 is running, the functional liquid will enter the liquid bottle. At this time, increasing the power or start-up rate of the semiconductor refrigeration chip can increase the acceleration rate of the functional liquid. The functional liquid exchanges more heat with the heating end of the semiconductor refrigeration chip, and the cooling capacity output by the cooling end of the semiconductor refrigeration chip is also greater. In this way, while lowering the temperature of the insulation space, the temperature of the functional liquid entering the liquid bottle is also increased.

[0140] Optionally, the liquid adding device 400 further includes a weighing module 450 , and the weighing module 450 is used to obtain the weight of the liquid container 413 .

[0141] The weight obtained by the weighing module 450 represents the residual amount of the functional liquid. Such a setting is beneficial for the automated cell culture workstation or the user to add the functional liquid or replace the liquid storage container in time.

[0142] Optionally, the peristaltic pump 440 is disposed outside the operating cabin.

[0143] This facilitates maintenance of the peristaltic pump 440 .

[0144] Optionally, the liquid outlet pipe includes a first part connected to the peristaltic pump 440 and a second part located in the operation cabin, and the first part and the second part are connected by a quick connector.

[0145] With such a configuration, the portion of the liquid adding module located outside the operating cabin and the portion located inside the operating portion can be quickly separated, making it convenient for the user to maintain the liquid adding module.

[0146] Optionally, the liquid adding device 400 includes multiple liquid containers 413 and multiple peristaltic pumps 440, and the multiple liquid containers 413 and the multiple peristaltic pumps 440 are correspondingly connected through multiple liquid inlet pipes and multiple liquid outlet pipes; the liquid adding device 400 includes multiple liquid bottles and multiple liquid adding heads 430.

[0147] In this way, the liquid adding device 400 can store more types of functional liquids, thus providing conditions for the automated cell culture workstation to realize more cell culture functions.

[0148] Optionally, a plurality of liquid adding heads 430 are connected, and when the liquid adding heads 430 avoid the liquid bottles, at least one liquid adding head 430 is located between two adjacent liquid bottles.

[0149] The multiple liquid adding heads 430 are connected, and the multiple liquid adding heads 430 can be moved simultaneously by only one driving device, which simplifies the equipment structure and reduces the equipment cost. As an implementation method, the multiple liquid bottles are arranged in a row, and the multiple liquid adding heads 430 are arranged in a row. When the multiple liquid adding heads 430 move parallel to the multiple liquid bottles, they only need to move the distance of two adjacent liquid bottles to make all the liquid adding heads 430 avoid all the liquid bottles, which reduces the travel required by the multiple liquid adding heads 430 and reduces the space occupied by the liquid adding device 400.

[0150] Optionally, a plurality of liquid bottles are used to store at least one of a culture medium, a digestion solution, a cleaning solution and a stop solution.

[0151] Culture medium is a special liquid used for cell culture, containing nutrients required for cell growth. Digestion solution can make cells detach from the adhesion surface and form single cells. Cleaning solution is used to remove impurities and contaminants in cell culture. Stop solution can control the progress of color development reaction in the experiment and facilitate subsequent absorbance measurement. This allows the automated cell culture workstation to realize a variety of different cell culture processes.

[0152] Optionally, the liquid adding device 400 further includes a second temperature control module 460, and the second temperature control module 460 is used to control the temperature of the liquid bottle at a second temperature.

[0153] Functional liquid and cells can only function at a specific temperature. For example, the second temperature is 37 degrees Celsius. The second temperature control module 460 is provided to reduce the activity of the cell fluid due to the decrease in temperature. Such a setting is conducive to cell culture.

[0154] Optionally, the second temperature control module 460 includes a heating ring, which is disposed on the first bottle rack 421, and the liquid bottle is suitable for being placed in the heating ring.

[0155] In this way, the contact area between the functional liquid in the liquid bottle and the second temperature control module 460 can be increased, thereby increasing the heating speed.

[0156] Optionally, the automated cell culture workstation further includes a second bottle rack 470 and a transfer device 480 , wherein the second bottle rack 470 is disposed in the operation cabin; and the transfer device 480 is used to transfer liquid bottles between the first bottle rack 421 and the second bottle rack 470 .

[0157] The first bottle rack 421 is located at a position where liquid can be easily added, and the second bottle rack 470 is located at a position where the functional liquid is used. The distributed layout of the liquid adding device 400 can make the automated cell culture workstation have a smoother functional liquid replenishment process without occupying more internal space.

[0158] Optionally, the automated cell culture workstation includes a base plate 500, a centrifuge 510 and a large robotic arm 360, wherein the base plate 500 is recessed downward to form a centrifugal area; the centrifuge 510 is installed in the centrifugal area; the large robotic arm 360 is arranged on the base plate 500, and the large robotic arm 360 is used to add test tubes to the centrifuge 510 or take out test tubes from the centrifuge 510.

[0159] In the embodiment of the present disclosure, the automated cell culture workstation includes one or more chambers, each chamber having a bottom plate 500 , which also serves as the bottom plate 500 of the automated cell culture workstation.

[0160] The bottom plate 500 is in a downwardly concave form, thereby forming an independent centrifugal region. Therefore, the bottom plate 500 and the multiple walls of the centrifugal region are an integrated structure. The integrated structure can be formed by bending and connecting multiple sheet metal parts, or by stamping a structural part.

[0161] The centrifuge 510 is disposed in the centrifugal area, so the bottom of the centrifuge 510 is lower than the plane of the bottom plate 500. The large mechanical arm 360 is disposed on the bottom plate 500, and the bottom of the centrifuge 510 is lower than the bottom of the large mechanical arm 360. The large mechanical arm 360 is used to take and place the centrifuge tube from the rotor of the centrifuge 510.

[0162] The automated cell culture workstation provided by the embodiment of the present disclosure occupies less space inside the cell culture workstation because the bottom of the centrifuge 510 is lower than the bottom plate 500; the centrifugal area is formed by the bottom plate 500 being recessed downward, and the automated cell culture workstation has a better sealing effect; the centrifugal area is formed by the bottom plate 500 being recessed downward, and the wall surface of the centrifugal area serves as the shell of the centrifuge 510, which reduces the volume of the centrifuge 510, thereby increasing the operating space inside the cell culture workstation.

[0163] Optionally, the rotor of the centrifuge 510 is lower than the plane where the bottom plate 500 is located; or, the rotor of the centrifuge 510 is flush with the bottom plate 500 .

[0164] The rotor of the centrifuge 510 is lower than the bottom plate 500 or flush with the bottom plate 500, and the centrifuge 510 will not interfere with the rotational movement of the robot arm, which facilitates the robot arm to transfer materials.

[0165] Optionally, the rotor of the centrifuge 510 has a plurality of centrifugal positions, and after the centrifuge 510 is finished working, it is reset to a preset angle so that the plurality of centrifugal positions are at preset positions.

[0166] The centrifuge 510 is provided with a plurality of centrifugal positions, and a plurality of centrifuge tubes can be centrifuged at the same time. Before the centrifuge 510 is started, the rotor has a clear preset position. In this position, it is convenient for the robot arm to place the centrifuge tube in the centrifuge 510. After the centrifuge 510 is centrifuged, the rotor returns to the preset position, which is convenient for the robot arm to take out the centrifuge tube from the centrifuge 510.

[0167] Optionally, the preset angle is one angle; or, the preset angle is a plurality of angles corresponding to a plurality of eccentric positions.

[0168] When the preset angle is one angle, the robot arm presets multiple positions corresponding to multiple centrifugal positions. When placing centrifuge tubes, the rotor does not move, and the robot arm moves between the multiple positions to place multiple centrifuge tubes.

[0169] When the preset angle is multiple angles, the robot arm presets a centrifuge tube pick-up and placement position. When placing the centrifuge tube, the target centrifugal position is located at the centrifuge tube pick-up and placement position of the robot arm through the rotation of the rotor.

[0170] Such a configuration is advantageous for the coordination between the robot arm and the centrifuge 510 for taking and placing the centrifuge tubes.

[0171] Optionally, the automated cell culture workstation further comprises a sleeve rack, which is used to place the sleeve and limit the sleeve in the axial direction; the robotic arm is also used to place the centrifuge tube into the sleeve and take the centrifuge tube out along a radial direction of the sleeve rack together with the sleeve.

[0172] The casing rack is used to place the casing. The centrifuge tube with a smaller diameter is inserted into the casing, and the overall diameter is larger, so that it can match the space of the centrifugal position. With such an arrangement, the centrifuge 510 can centrifuge centrifuge tubes of different sizes at the same time, thereby improving the scope of application of the centrifuge 510.

[0173] The sleeve rack limits the movement of the sleeve in the axial direction. When using the sleeve, the mechanical arm clamps the centrifuge tube and inserts it into the sleeve along the axis. Since the movement of the sleeve along the axial direction is limited, the friction between the centrifuge tube and the sleeve will not cause the sleeve to move. After inserting the sleeve, the clamp arm 264 moves the sleeve and the centrifuge tube together radially outward along the sleeve, so as to take out the centrifuge tube with the sleeve. When removing the sleeve, the large mechanical arm 360 puts the centrifuge tube with the sleeve back to the sleeve rack along the radial direction of the sleeve, and the large mechanical arm 360 clamps the centrifuge tube and moves it along the axial direction of the sleeve, so that the sleeve can be returned to its position and the centrifuge tube can be pulled out. With such a setting form, the automated cell culture workstation can automatically complete the operation of sleeve-fitting or sleeve-removing the centrifuge tube.

[0174] The working cabin 100 for a cell culture workstation includes a shell, a first high-efficiency filter 122, a disinfection pipe 130 and a purification fan 123, wherein the shell defines an operation area 350 and a purification duct 121, and air flows from the purification duct 121 to the operation area 350; the first high-efficiency filter 122 is arranged in the purification duct 121, and the air in the purification duct 121 flows to the operation area 350 through the first high-efficiency filter 122; the disinfection pipe 130, the release end of which is located in the purification duct 121, and the disinfection gas released through the disinfection pipe 130 enters the operation area 350 along with the air in the purification duct 121 through the first high-efficiency filter 122; the purification fan 123 is used to drive the air to flow from the purification duct 121 to the operation area 350.

[0175] The working chamber 100 provided in the embodiment of the present disclosure may be used as a transfer chamber 322 , a consumable chamber or a culture chamber 332 of a cell culture workstation.

[0176] The working cabin 100 includes a shell, and the operating area 350 domain and the purification air duct 121 are defined inside the shell. The operating area 350 domain is compared with the purification air duct 121. For example, when the working cabin 100 is used as a culture cabin 332, the operating area 350 domain is used as the culture area 330 domain; when the working cabin 100 is used as a transfer cabin, the operating area 350 domain is used as an area for transferring materials. The working cabin 100 has corresponding air duct structures when used as different functional cabins.

[0177] A first high efficiency filter 122 is arranged in the purification air duct 121 inside the housing. In the housing, the air flows from the purification air duct 121 to the operation area 350. The power to drive the air flow comes from the purification fan 123.

[0178] After the air in the purified air duct 121 passes through the first high-efficiency filter 122 , dust, impurities, bacteria, microorganisms, etc. in the air are filtered and adsorbed, and the clean air enters the operating area 350 .

[0179] The working cabin 100 also includes a disinfection pipe 130, the release end of which is located in the purification air duct 121 and in front of the first high-efficiency filter 122 along the air flow direction. In the first aspect, the disinfection gas released by the disinfection pipe 130 can also sterilize the first high-efficiency filter 122, reducing or avoiding the first high-efficiency filter 122 from breeding bacteria and contaminating the air in the operation area 350 domain. In the second aspect, the disinfection gas enters the operation area 350 domain through the first high-efficiency filter 122, which can make the disinfection gas fully mixed with the air of the purification air duct 121 before entering the operation area 350 domain, reducing or avoiding the uneven distribution of disinfection gas in the operation area 350 domain and causing disinfection dead corners. In the third aspect, the disinfection gas enters the operation area 350 domain through the first high-efficiency filter 122, and even if the release pressure of the disinfection gas is large, it can be decelerated through the first high-efficiency filter 122, and the air flow rate entering the operation area 350 domain through the first high-efficiency filter 122 is uniform, and the air flow in the operation area 350 domain is stable and not easy to be involved in the gas of the external environment.

[0180] When using the working cabin 100 provided in the embodiment of the present disclosure, the disinfection gas can also disinfect the first high-efficiency filter 122, reducing or avoiding the growth of bacteria in the first high-efficiency filter 122; the release end of the disinfection pipe 130 is located before the first high-efficiency filter 122, and the disinfection gas and the air in the purification duct 121 can be more fully mixed through the first high-efficiency filter 122, thereby reducing the concentration difference of the disinfection gas in the operating area 350 and reducing the blind spots of disinfection; the disinfection gas released from the disinfection pipe 130 is decelerated after passing through the first high-efficiency filter 122, and the air flow rate entering the operating area 350 is uniform, which can reduce or avoid the involvement of external air.

[0181] Optionally, the shell also defines an exhaust duct 141, a first end of the exhaust duct 141 is connected to the operating area 350, and a second end can be selectively connected to the external environment or the purification duct 121; the work cabin 100 also includes an exhaust fan 142, which is arranged in the exhaust duct 141, and the exhaust fan 142 is used to drive air to flow from the operating area 350 to the exhaust duct 141.

[0182] For the working cabin 100, the air can be in an external circulation mode or an internal circulation mode. In the external circulation mode, the air from the external environment enters the operation area 350 through the purification duct 121, and the air in the operation area 350 is discharged to the external environment through the exhaust duct 141. In the internal circulation mode, the air flows through the purification duct 121, the operation area 350 and the exhaust duct 141 in sequence, and the air entering the exhaust duct 141 is controlled to flow back to the purification duct 121.

[0183] In the external circulation mode, the operating area 350 can be ventilated quickly. In the internal circulation mode, the sterilizing gas circulates back and forth, which can reduce the amount of sterilizing gas used and reduce or avoid the contamination of materials and the pollution of the environment by the exhaust air. With such a setting, the working cabin 100 can switch between the internal circulation mode and the external circulation mode.

[0184] Optionally, the sterilization pipe 130 is passed with hydrogen peroxide gas.

[0185] Hydrogen peroxide gas can kill most common microorganisms at room temperature, and has good penetration into the first high-efficiency filter 122, and can achieve the effect of biological decontamination. Hydrogen peroxide gas disinfection is environmentally friendly, and its final residues are water and oxygen, which does not pollute the environment.

[0186] Optionally, the working cabin 100 is also provided with an ultraviolet disinfection lamp.

[0187] The ultraviolet disinfection lamp can complement the disinfection of hydrogen peroxide, and this setting can improve the disinfection ability of the working cabin 100.

[0188] Optionally, the working cabin 100 further includes a second high efficiency filter 143 , which is disposed in the exhaust air duct 141 , and the air in the operating area 350 enters the exhaust air duct 141 and flows through the second high efficiency filter 143 .

[0189] A second high-efficiency filter 143 is provided, which can perform two-stage purification and filtration on the air in the internal circulation mode, and can reduce the pollution of the exhaust air to the environment in the external circulation mode.

[0190] In addition, in both the internal circulation mode and the external circulation mode, the disinfectant gas can sterilize the second high-efficiency filter 143. In the internal circulation mode, the second high-efficiency filter 143 can further mix the disinfectant gas with the air pre-entering the operation area 350, so that the disinfectant gas can sterilize the operation area 350 more comprehensively.

[0191] Optionally, the exhaust volume of the exhaust air duct 141 is smaller than the intake volume of the purification air duct 121 .

[0192] In this way, the working cabin 100 can maintain a positive pressure environment. As an implementation method, the fan power of the exhaust air duct 141 is less than the power of the purification air duct 121. Reduce or prevent the air from the external environment from entering the working cabin 100. As another implementation method, the air resistance of the second high-efficiency filter 143 is greater than the air resistance of the first high-efficiency filter 122. In this way, the air volume of the exhaust air duct 141 can also be slightly smaller than the air volume of the purification air duct 121, thereby maintaining the positive pressure environment of the working cabin 100. Maintaining a positive pressure environment in the operating area 350 can further reduce or prevent the outside air from entering the interior of the automated cell culture workstation.

[0193] Optionally, the purification air duct 121 is provided with an air vent 144, which can be opened and closed under control. When the air vent 144 is closed and the second end of the exhaust air duct 141 is connected to the purification air duct 121, the working cabin 100 performs internal circulation; when the air vent 144 is opened and the second end of the exhaust air duct 141 is connected to the external environment, the working cabin 100 performs external circulation.

[0194] With such a configuration, the switching between the internal circulation mode and the external circulation mode of the working cabin 100 is more convenient.

[0195] Optionally, the shell includes a top plate, multiple side plates, a first partition plate 111 and a second partition plate 112, wherein the multiple side plates and the top plate enclose a accommodating space; the first partition plate 111 is vertically arranged in the accommodating space, and the first partition plate 111 and the side plates define an exhaust air duct 141; the second partition plate 112 is horizontally arranged in the accommodating space, and the second partition plate 112 and the top plate define a purification air duct 121; wherein the first partition plate 111 or the second partition plate 112 is provided with a connecting port 146, and the connecting port 146 can be controlled to open and close, and when the connecting port 146 is opened, the exhaust air duct 141 is connected to the purification air duct 121.

[0196] The second partition 112 is arranged horizontally, and the purification air duct 121 is located at the top of the operating area 350. In this way, the air mixed with the disinfection gas can enter the operating area 350 at an angle that makes it easier to cover the operating area 350. The first partition 111 is arranged vertically, and the exhaust air duct 141 is located on one side of the operating area 350. In this way, the operable area 350 of the operating area 350 can be increased. As an optional embodiment, the top of the first partition 111 abuts against the downward side of the second partition 112. In this case, the connecting port 146 is opened in the first partition 111. As another optional embodiment, one end of the second partition 112 abuts against the side of the first partition 111 facing the operating area 350. In this case, the connecting port 146 is opened in the first partition 111. This embodiment is conducive to providing opening and closing facilities such as a damper assembly for the connecting port 146.

[0197] Optionally, an exhaust port 145 is provided in an area of ​​the top plate corresponding to the exhaust air duct 141 , and the exhaust port 145 can be opened and closed under control, and an air inlet 144 is provided in an area of ​​the top plate corresponding to the purification air duct 121 .

[0198] In the external circulation mode, the purification air duct 121 introduces outside air through the air inlet 144, and the exhaust air duct 141 exhausts air to the outside through the air outlet 145. The air inlet 144 and the air outlet 145 are both provided on the top plate, which is conducive to the overall layout of the automated culture workstation.

[0199] Optionally, the communication port 146 is opened at the bottom of the first partition plate 111 .

[0200] For the operation area 350, the air enters from the top and exits from the bottom, which not only increases the coverage of the disinfection gas, but also facilitates the orderly flow of air, thereby reducing the involvement of outside air.

[0201] Optionally, the working cabin 100 further includes a flow balancing membrane 150 , which is disposed in the operating area 350 , and the air in the purified air duct 121 flows through the first high-efficiency filter 122 and the flow balancing membrane 150 in sequence.

[0202] The flow-evening membrane 150 is evenly provided with a plurality of flow-evening holes. After the air passes through the first high-efficiency filter 122 and then flows through the flow-evening membrane 150, the air mixed with the sterilizing gas can enter the operation area 350 more evenly.

[0203] Optionally, the disinfection pipe 130 passes through the shell so that the release end is located in the purification air duct 121 and the other end opposite to the release end is located outside the shell.

[0204] The purification air duct 121 has a small space. If the sterilizing gas storage device or generating device is set in the purification air duct 121, it will cause inconvenience in operation and maintenance. The sterilizing pipe 130 runs through the shell, so that the position of the sterilizing gas storage device or generating device connected to the sterilizing pipe 130 is not affected by the use position and can be set at other positions. This not only allows the operation area 350 to obtain a larger available space, but also facilitates the maintenance of the sterilizing gas storage device or generating device.

[0205] An embodiment of the present disclosure provides an automated cell culture workstation, which includes a consumables area 320, an operation area 350 and a culture area 330, wherein the operation area 350 is connected to the consumables area 320; the culture area 330 is connected to the operation area 350; the consumables area 320, the operation area 350 or the culture area 330 includes the above-mentioned work cabin 100.

[0206] The automated cell culture workstation provided by the embodiment of the present disclosure includes a consumables area 320 , an operation area 350 , and a culture area 330 .

[0207] The consumable area 320 includes a transfer compartment and a consumable compartment. The transfer compartment is provided with a first door. When the first door is opened, the transfer compartment is connected to the environment, allowing materials to be automatically or manually stored in the transfer compartment. A second door is provided between the consumable compartment and the transfer compartment. When the second door is opened, the automated cell culture workstation can automatically transfer materials to the consumable compartment.

[0208] Optionally, the above-mentioned working cabin 100 is used as a transfer cabin.

[0209] When placing materials into the transfer chamber, the first door is opened and the second door is closed. At this time, the transfer chamber is preferably switched to the external circulation mode. Clean air enters the transfer area of ​​the transfer chamber through the purification air duct 121. The air entering the transfer chamber is discharged to the external environment through the exhaust air duct 141. In the external circulation mode, the air in the transfer chamber can be quickly updated.

[0210] After the materials are placed in the transfer chamber, the transfer chamber is preferably switched to an internal circulation mode. In this mode, the materials are sterilized by circulating air mixed with the sterilizing gas.

[0211] After the first preset time, the second hatch opens and the materials are transferred to the consumables compartment.

[0212] Optionally, the above-mentioned working chamber 100 is used as a consumables chamber.

[0213] After the materials enter the consumables compartment, the consumables compartment is preferably switched to an internal circulation mode, whereby the consumables are continuously disinfected by circulating disinfectant gas and a clean environment is maintained for the consumables compartment.

[0214] The operation area 350 of the automated cell culture workstation includes a transfer chamber 310, an operation area 350 and a batch processing chamber.

[0215] The above-mentioned working chamber 100 can also be used as the transfer chamber 310 operation area 350 and the batch processing chamber.

[0216] The culture area 330 of the automated cell culture workstation includes a culture chamber 332 , and the above-mentioned work chamber 100 can also serve as the culture chamber 332 .

[0217] Optionally, the automated cell culture workstation includes a plurality of the above-mentioned working chambers 100 , and the sterilization pipes 130 of the plurality of working chambers 100 are interconnected.

[0218] In this way, disinfection of multiple work areas can be achieved by setting up fewer disinfection gas storage devices or generation devices, thereby reducing costs and improving efficiency.

[0219] Optionally, the exhaust ducts of multiple working cabins 100 are interconnected.

[0220] In this way, the exhaust gases from multiple chambers can be discharged centrally, for example, discharged to the atmosphere after centralized secondary treatment. Such an arrangement is conducive to centralized treatment of exhaust gases.

[0221] Optionally, the purification air ducts 121 of multiple compartments are interconnected.

[0222] This helps to maintain a balanced air pressure between the multiple working cabins 100, reduces unnecessary air flow, and thus alleviates possible pollution caused by air flow.

[0223] Optionally, the first high efficiency filter 122 is disposed laterally.

[0224] In this way, the first high-efficiency filter 122 can be placed at a better windward angle and wind outlet angle, thereby increasing the air volume of the purification air duct 121.

[0225] The above description and the accompanying drawings sufficiently illustrate the embodiments of the present disclosure to enable those skilled in the art to practice them. Other embodiments may include structural and other changes. The embodiments represent only possible variations. Unless explicitly required, individual components and functions are optional, and the order of operations may vary. Portions and features of some embodiments may be included in or replace portions and features of other embodiments. The embodiments of the present disclosure are not limited to the structures described above and shown in the accompanying drawings, and various modifications and changes may be made without departing from the scope thereof. The scope of the present disclosure is limited only by the appended claims.

Claims

1. An automated cell culture workstation, characterized in that: include: The transfer room is equipped with a large robotic arm; a consumables area, connected to the transfer chamber and located at a first side of the transfer chamber; a culture area, connected to the transfer chamber and located at a second side of the transfer chamber; an analysis area, connected to the transfer chamber and located at a third side of the transfer chamber; an operation area, connected to the transfer chamber and located at a fourth side of the transfer chamber; Wherein, the large robotic arm is used to transfer materials between the consumables compartment, the culture area, the analysis area and the operation area.

2. The automated cell culture workstation according to claim 1, characterized in that: The first side is opposite to the second side, and the third side is opposite to the fourth side.

3. The automated cell culture workstation according to claim 1, characterized in that: The culture area comprises: A transfer cabin, one end of which is connected to the transfer chamber; A plurality of culture chambers are arranged on one side or both sides of the transfer chamber. The culture chambers are provided with automatic doors. When the automatic doors are opened, the corresponding culture chambers are connected with the transfer chamber.

4. The automated cell culture workstation according to claim 3, characterized in that: The culture area also includes: An automatic slide rail, arranged in the transfer cabin, the automatic slide rail covers the automatic doors of the multiple culture cabins; The transfer trolley is slidably arranged on the automatic slide rail.

5. The automated cell culture workstation according to claim 4, characterized in that: The culture area also includes: An incubator turntable rack is arranged in the incubator cabin; Incubator pick-and-place device, used to transfer culture bottles between the transfer trolley and the incubator turntable rack.

6. The automated cell culture workstation according to any one of claims 1 to 5, characterized in that: The consumables area includes: A loading chamber, connected to the transfer chamber, and used for temporarily storing materials; The transfer room is communicated with the loading room, and the transfer room is provided with a door connected to the external environment.

7. The automated cell culture workstation according to claim 6, characterized in that: A glove compartment is provided on the outward side of the loading chamber; and / or, A glove compartment is provided on the outward side of the operation area; and / or, A glove compartment is arranged on the outward side of the analysis area.

8. The automated cell culture workstation according to claim 6, characterized in that: The consumables area also includes: The material slide rail has a travel that covers the loading chamber and the transfer chamber, and the material slide rail is used to transfer materials between the loading chamber and the transfer chamber.

9. The automated cell culture workstation according to claim 8, characterized in that: The consumables area also includes: A consumables turntable rack is arranged in the loading chamber; A pick-and-place device is used to transfer consumables between the consumable turntable rack and the transfer chamber.

10. The automated cell culture workstation according to any one of claims 1 to 5, characterized in that: Also includes: An automatic counting device, arranged in the analysis area; and / or, a centrifuge, disposed in the transfer chamber; and / or, The liquid adding module is arranged in the operation area.

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