Method and device for treating adipose tissue

Abstract

The present invention relates to a method suitable for obtaining selectively variable particle size fat-based compounds, characterized by high purity and high concentration of viable mesenchymal stem cells, which can be used in autotransplantation procedures primarily for regeneration purposes, as well as for filling and / or increasing volume purposes, as well as to a device that can implement the method.

Description

Technical Field

[0001] The present invention relates to the field of methods and devices for producing fat-based compounds intended for reimplantation into the same donor patient in a single-stage procedure using both surgical and outpatient approaches.

[0002] More in detail, the present invention relates to a method and a device suitable for obtaining a selective variable-size fat-based compound characterized by high purity and high concentration of viable mesenchymal stem cells, which can be used for autologous transplantation procedures mainly for regenerative purposes, as well as for filling and / or volume-increasing purposes. Background Art

[0003] Regenerative medicine is a vast biomedical and clinical field that encompasses many medical and surgical specialties, all of which aim to treat degenerative diseases or phenomena of aging, and to regenerate organs and tissues.

[0004] Recent advances in research in this area stem from the therapeutic potential that can be foreseen when using multiple factors with biological activity and cells with direct regenerative effects, i.e., able to differentiate in the recipient tissue / organ (paracrine) and able to awaken the regenerative capacity of local cells collected from the same subject.

[0005] After the experience gained with the placenta and the bone marrow, it has been seen how adipose tissue, which is usually available and easy to collect, forms the largest reserve of regenerative elements and MSCs (mesenchymal stem cells) to date. These are undifferentiated multipotent cells that have not yet acquired specific functions and retain the ability to direct their evolution. These cells constitute the basis of regenerative medicine due to their therapeutic potential, which includes the inhibition of inflammation and apoptosis, the stimulation of angiogenesis, the recruitment and the aforementioned differentiation capacity.

[0006] The development of regenerative medicine has been combined with the practice of lipografting, a very common minimally invasive fat transplantation procedure with widespread applications in reconstructive and cosmetic plastic surgery. In this practice, adipose tissue has long been used solely for the purpose of volume addition, despite high variability in implantation and unpredictable or sometimes even no results. All this occurred before its regenerative biological potential was understood.

[0007] To date, the lack of standardized and widely shared protocols for harvesting, processing, and transplantation is considered the greatest sticking point in fat grafting procedures. Recent discoveries in the field of tissue regeneration have greatly fueled the research and development of new methods to not only better understand the composition of harvested adipose tissue, but also to optimize the regenerative bioactivity of fat implants and transplant products.

[0008] To date, stem cells can be obtained in high concentrations from adipose tissue either by enzymatic digestion from the stromal vascular fraction (SVF) or by mechanical processing of the fat contained in the adipose tissue.

[0009] The enzymatic digestion method has an important negative impact, as it is only permitted for research purposes by regulatory agencies (AIFA, EMA, FDA) and uses laboratory procedures performed in clean rooms, which determines a delayed implantation in patients after collection. In addition to this, there is an even more important negative impact, as many recent studies have shown a decrease in the activity of stem cells obtained with this method.

[0010] Instead, regulators allow the use of mechanical extraction methods, and, because it can be performed at the time of collection in the operating room or during an outpatient procedure, the stem cells obtained from the original adipose tissue can be implanted immediately after collection.

[0011] Currently, the main scientific literature tends to use the whole fat structure collected according to the concept of Nanofat, where the fragments are less than 600 microns. This determines the important limitation that excludes the use of products with higher fragments such as Millifat and Microfat, unless the volume is larger.

[0012] With the exception of centrifugation solutions, which are slowly being abandoned, current mechanical extraction solutions have some important negative effects:

[0013] They require manual compression forces to pass and force homogenization of the collected adipose tissue, multiple passes through syringes through different connectors, which results in high cell stress and reduced viability and survival of the stem cells thus obtained, as well as the formation of new oily residues due to adipocyte rupture;

[0014] They are operator dependent, and the operator can apply different manual compression forces to the harvested adipose tissue;

[0015] They require multiple passes of the collected adipose tissue from one syringe to another, which results in an increased potential for contamination of the final product;

[0016] Liquid residues can only be removed from the final product by decantation;

[0017] Fibrous and / or oily and / or bloody residues in the final product are not disposed of. Summary of the invention

[0018] The object of the present invention is to propose a method and a device capable of implementing the method, capable of overcoming the above-mentioned limitations and disadvantages of current methods for extracting stem cells from adipose tissue, thereby facilitating obtaining fat-based compounds with selectively variable particle size, characterized by high purity and high concentration of viable mesenchymal stem cells.

[0019] The present invention also aims to provide a method having the following characteristics:

[0020] maximum standardization of fat processing procedures directly related to collection procedures;

[0021] Maximum standardization and constant reproducibility of results;

[0022] Performed in a closed circuit or a circuit that ensures maximum sterility;

[0023] Independent of the operator;

[0024] Elimination of fibrous and / or oily and / or bloody residues from the obtained stem cells;

[0025] The concentration of stem cells obtained;

[0026] Minimize cellular stress to maintain the viability of the obtained stem cells;

[0027] Selection of particle size of the final product;

[0028] Remove excess liquid from the final product.

[0029] Among the above characteristics, eliminating residues and reducing cell stress in the final product are crucial because impurities and cell stress can significantly reduce the survival rate of mesenchymal stem cells (MSCs).

[0030] The object of the invention is achieved by a method for treating adipose tissue according to the main independent claim 1 .

[0031] The invention also relates to a device for treating adipose tissue according to independent claim 6 .

[0032] Further features of the method and device of the invention are described in the dependent claims.

[0033] The method and apparatus of the present invention yield a number of important advantages, such as:

[0034] The processing sequence of the collected adipose tissue, providing a first physico-mechanical processing step and a second first physical processing step, allows complete objectivity and complete reproducibility of the treatments performed;

[0035] The two processing steps implemented can be performed very easily by means of filter columns and the vacuum dewatering filter equipment associated therewith;

[0036] By means of the filter column and the vacuum dehydration filter device, the plant layout configuration is very simple and can be used without the need for particularly professional operators;

[0037] By means of the filter column and the vacuum dehydration filter device, the plant layout configuration is suitable for the recovery of both nano fat (which is also processed by the vacuum dehydration filter) and micro fat and milli fat (which are only processed by the filter column), so that the treatment implemented is highly adaptable and flexible;

[0038] The vibration sieving treatment is carried out by means of an oscillating type radial agitator associated with the filter column, avoiding the transmission of beating movements, traction, compression or centrifugation to the treated adipose tissue, thereby reducing its mechanical stress and thus increasing its viability and the viability of the stem cells obtained therefrom.

[0039] Other detailed aspects which confer further advantages to the present invention are:

[0040] The total duration of the adipose tissue enhancement procedure is less than 5 minutes: the limited time therefore has the great advantage of minimizing the stress to which the treated adipose tissue is subjected;

[0041] Protective synergy of hyaluronic acid: the greatest advantage of the proposed technology is that any mechanical compression / expansion stress on the cell material is minimized during the particle size selection process, while hyaluronic acid, due to its important viscoelastic properties, has the ability to cooperate with the process of forming a protective film that helps the cell material pass through the metal mesh, greatly reducing stress;

[0042] An important purpose of the vacuum filtration process is to extract the excess water present in the nanofat fraction: this treatment is useful and necessary because, as indicated in international scientific literature, the removal of the cellular material of the adipose tissue results in the release of extracellular water which, in this step, is free and must therefore be eliminated. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Further features and advantages of the present invention will become more apparent from the more detailed description set out below, with the aid of the accompanying drawings showing preferred embodiments illustrated by way of non-limiting examples, in which:

[0044] Figure 1 All implementation steps of the method for processing adipose tissue according to the present invention are shown in the form of a schematic flow chart;

[0045] Figure 2 A possible variation of the embodiment of the above method is shown in the form of a schematic flow chart;

[0046] Figure 3 and Figure 4 Schematically shows a plant layout for implementing the above-described method and possible variants of its embodiment;

[0047] Figure 5 , 6 7 and 8 show some graphs related to experimental results obtained using the method and apparatus of the present invention. DETAILED DESCRIPTION

[0048] See also Figure 1 According to the present invention, the method for treating adipose tissue basically comprises the following steps:

[0049] providing a plurality of screens having progressively decreasing mesh sizes;

[0050] Providing a radial agitator device of the oscillating type;

[0051] Provide vacuum dehydration filter unit;

[0052] Adipose tissue T1 was collected from donor patients;

[0053] The adipose tissue T1 is subjected to a first physical-mechanical treatment step through a plurality of sieves with gradually decreasing mesh sizes, and at least one oversize fraction containing fiber fractions of different particle sizes and a remaining wet and homogenized undersize fraction are separated by the oscillating radial agitator device, wherein the undersize fraction is a nanofat type rich in stem cells;

[0054] At least the remaining wet and homogenized undersize fraction is subjected to a second physical treatment step by means of the vacuum dehydration filter device to eliminate water in order to concentrate the fraction and obtain adipose tissue T2a of nano-fat particle size, free from fibrous and / or oily and / or blood residues R1 and water residues R2, containing a high concentration of viable mesenchymal stem cells, wherein the adipose tissue T2a is suitable for being intended for re-implantation into the same donor patient.

[0055] Specifically, the first physical and mechanical treatment step includes the following steps:

[0056] A filter column 1 is provided, which is provided with at least a first and a second screen 3, 4 placed in sequence, the mesh size of which decreases in the direction of the filtration flow;

[0057] The filter column 1 is operated by means of a radial agitator device of the oscillating type;

[0058] The adipose tissue T1 is subjected to impurity separation and homogenization treatment through the first sieve 3 to obtain an oversize material SP and an undersize material ST;

[0059] Disposal of fiber and / or oily and / or blood residues R1 contained in the oversize SP of the first screen 3;

[0060] The undersize ST of the immediately preceding sieve is subjected to impurity separation and homogenization treatment by the second sieve 4 to obtain an oversize portion T2b and a wet undersize portion ST including nano-fat tissue T2a and water residue R2.

[0061] Specifically, the second physical treatment step includes the following steps in sequence:

[0062] A vacuum dehydration filter device 2 is provided, provided with a filter 8;

[0063] Providing a suction pump 9 suitable for operating the vacuum dehydration filter device;

[0064] The remaining wet and homogenized undersize fraction ST of the second sieve 4 is subjected to a separation treatment of the water residue R2 by the filter 8 and the suction pump 9 of the vacuum dehydration filter device 2, thereby obtaining a fat tissue T2a of nano-fat particle size without the water residue R2.

[0065] See also Figure 1 According to a first variant of the invention, the method for treating adipose tissue comprises the following further steps:

[0066] The sieve material SP of the second sieve 4 is collected, including adipose tissue T2b with microfat particles, which naturally has a low moisture content and is free of fiber and / or oily and / or blood residues R1, and contains a high concentration of viable mesenchymal stem cells, wherein the adipose tissue T2b with microfat particles is suitable for being intended for re-implantation into the same donor patient.

[0067] See also Figure 2 According to another variant of the invention, the method for treating adipose tissue comprises the following further steps:

[0068] providing a third screen 7, which is placed between the first and second screens 3, 4, to obtain further oversize and further undersize;

[0069] The third screen 7 is used to separate the impurities and then homogenize the undersize material ST of the screen immediately before it;

[0070] The oversize material SP of the third sieve 7 is collected to obtain adipose tissue T2c with a millifat particle size, naturally low in water content and free from fibrous and / or oily and / or blood residues R1, containing a high concentration of viable mesenchymal stem cells, intended for reimplantation into the same donor patient.

[0071] Detailed description of the apparatus for carrying out the method of the invention

[0072] See also Figure 3 According to the present invention, the device for processing adipose tissue T1 collected from a donor patient basically comprises:

[0073] a filter column 1 provided with at least a first and a second screen 3, 4, the mesh size of which decreases in the direction of the filtration flow, adapted to be operated by a radial agitator device 5 of the oscillating type;

[0074] A vacuum dehydration filter device 2, provided with a filter 8, adapted to be operated by a suction pump 9,

[0075] wherein said filtration column 1, through said second sieve 4, allows obtaining an undersize of adipose tissue comprising moist nano-fat particles and an oversize of adipose tissue T2b comprising micro-fat particles, naturally having a low moisture content, and wherein said vacuum dehydration filter device 2 allows obtaining adipose tissue T2a without water residue R2, wherein each adipose tissue T2a, T2b is free of fibrous and / or oily and / or blood residues R1, contains a high concentration of viable mesenchymal stem cells, and at least one of these adipose tissues is intended for re-implantation into the same donor patient.

[0076] The first screen 3 and the second screen 4 define the top and bottom of the filter column 1, respectively.

[0077] Preferably, the first sieve 3 and the second sieve 4 comprise sieve holes with sizes of 2 mm and 0.6 mm, respectively.

[0078] The filter column 1 also includes:

[0079] a reversible closing cover 20 of the first screen 3 provided with a sealing point 21 for the insertion of the adipose tissue T1 which can optionally be combined and / or dosed with a substance having a therapeutic and synergistic effect, such as hyaluronic acid or the like;

[0080] The collection chamber 6 for the undersize material ST of the second screen 4 is provided with a flat bottom or a slightly inclined bottom.

[0081] According to a more complex possible embodiment, such as Figure 4 As shown, the filter column 1 may further include:

[0082] The third screen 7 is arranged in the middle between the first and second screens 3 and 4.

[0083] So as to allow obtaining a further oversize comprising adipose tissue T2c of milli-fat size, naturally having a low water content and free from fibrous and / or oily and / or blood residues R1, containing a high concentration of viable mesenchymal stem cells, destined for reimplantation into the same donor patient.

[0084] The third screen 7 defines the central part of the filter column 1 .

[0085] Preferably, the mesh size of the third sieve 7 is 1 mm.

[0086] The stirring means of the screens 3, 4 and 7 of the filter column 1 comprise a radial mixer 5, for example of the vortex mixer type, suitable for producing an oscillating rather than a pulsating movement, so as to obtain a vibrating sieving.

[0087] The vacuum dehydration filter device 2 also includes:

[0088] an upper part 10 provided with a reversible closing cover 22;

[0089] An atmospheric air A inlet 11 located on the closing cover;

[0090] A one-way filter valve device 23 combined with the inlet 11, suitable for filtering atmospheric air A;

[0091] The lower part 12 is provided with a tank 13 for collecting the water residue R2 separated from the undersize ST of the second screen ( 4 ) of the filtering column 1 .

[0092] Preferably, the filter 8 of the vacuum dewatering filter device 2 is made of paper or steel with a particle size between 20 and 25 microns.

[0093] Example 1

[0094] The treatment of the invention was tested in the laboratory to verify the enhancement of useful and "valuable" fractions T2a, T2b, T2c (containing viable mesenchymal stem cells) of adipose tissue T1, obtained by means of a physico-mechanical device and a physical device consisting of a filtration column 1, the purpose of which is to eliminate the fibrous and / or oily and / or blood fractions and to homogenize the remaining part of the adipose tissue T1, and a physical device consisting of a vacuum dehydration filter 2, the purpose of which is to eliminate the water present in the adipose tissue T1, so as to concentrate the valuable fraction T2a.

[0095] The filter column 1 is equipped with two screens 3, 4 or three screens 3, 7, 4, the mesh sizes of which decrease in the filtering direction, and are 2 mm and 0.6 mm respectively in the case of two screens, and 2 mm, 1 mm and 0.6 mm respectively in the case of three screens.

[0096] The filter column 1 is agitated by means of a radial mixer 5 (vortex mixer) which ensures only an oscillating movement without pulsating movements, thus allowing the treated fat material to penetrate in a non-damaging manner the meshes of the sieves 3, 4 and 7. This mixer 5 ensures the almost complete absence of traction and compression stresses and the complete absence of centrifugal forces which could cause the cellular material of the fat tissue to be stressed on the walls of the individual sieves 3, 4 and 7 of the filter column 1.

[0097] Furthermore, in order to allow the valuable portion T2a of the adipose tissue T1 to accumulate without damage, after passing through the screens (two or three) of the filter column 1, a cylindrical collection chamber 6 equipped with a flat bottom (or a slightly inclined bottom) is provided to prevent mechanical action of the upper layer of the cellular material of the valuable portion T2a of the adipose tissue T1 relative to the lower layer, which would occur if it had a truncated cone-shaped cross-section and would result in a stress state.

[0098] The vacuum dewatering filter device 2 is provided with a paper (or steel) filter 8 with a porosity between 20 and 25 micrometers, a suction pump 9 and an atmospheric air A inlet 11 combined with a filter valve device 23 for air.

[0099] Regardless of the configuration of the filter column 1 , the oversize SP of the first screen 3 (mesh size 2 mm) is sent for processing to dispose of fibrous and / or oily and / or blood residues R1 from the adipose tissue T1 .

[0100] In the configuration of the filter column 1 with two sieves, the undersize ST of the second sieve 4 (mesh size is 0.6 mm) accumulated in the collection chamber 6 is sent to the dehydration filter device 2 to finally obtain the adipose tissue T2a with nano-fat particles. In addition, the oversize SP of the second sieve 4 is collected to obtain the adipose tissue T2b with micro-fat particles.

[0101] In the configuration of the filtration column 1 having three sieves, the oversize SP of the third sieve 7 (mesh size of 1 mm) is also collected to obtain adipose tissue T2c of milli-fat particle size.

[0102] The vacuum dehydration filter device 2 determines the separation of the water residue R2 from the fat tissue T2a by the vacuum pressure generated by the flow of the moving filtered atmospheric air A from the upper part 10 to the lower part 12 of the device forced by the pump 9. This vacuum pressure allows the water residue R2 of the fat tissue T2a to pass through the filter 8 placed between the upper part 10 and the lower part 12 of the device and thus be deposited in the collection tank 13.

[0103] Then, adipose tissue T2a of nano-fat particle size, free from fibrous and / or oily and / or blood residues R1 and water residues R2, is collected from the filter 8 of the vacuum dehydration filter device 2, containing mesenchymal stem cells with high viability, since they are only subjected to limited stress from the sieves 3, 4, 7 of the filter column 1, so that it is possible to re-implant them into the same donor patient using methods and instruments of known type.

[0104] Likewise, adipose tissue T2b, T2c of micro- and milli-fat particle size, respectively, free of fibrous and / or oily and / or blood residues R1, is collected from the screens 4, 7 of the filter column 1 for possible reintroduction into the donor patient using methods and instruments of known type.

[0105] The adipose tissue T2b, T2c can be directly collected from the screens 4, 7 of the column 1, bypassing the vacuum dehydration filter, as follows:

[0106] It naturally has a low moisture content, which tends to accumulate mainly in the undersize ST of the second screen 4 of the filter column 1;

[0107] With a particle size larger than nanofat, vacuum dehydration filter processing causes its density to increase, making it difficult to reimplant it into the donor patient through a particularly thin cannula.

[0108] Example 2

[0109] The method and device of the present invention were verified using the following implementation method:

[0110] Adipose tissue T1 samples were collected from donor patients through a cannula with a diameter of 2.2 mm, a lumen of 2 mm, and multiple 1 mm holes at 360° to reduce collection pressure;

[0111] A 20cc syringe was used to perform tumescent aspiration of the adipose tissue T1 that was subjected to harvesting;

[0112] Regardless of the configuration of the filter column 1 , the collected adipose tissue T1 is inserted into the first mesh 3 ;

[0113] The stirrer 5 is started at a rotation speed of 2000 rpm for 1 minute. These values ​​are purely indicative and, above all, have no limiting value for the purpose of implementing the method of the invention or using the device of the invention;

[0114] After filtration, the portion T2a of the adipose tissue T1 accumulated in the collection chamber 6 is sent to the vacuum dehydration filter device 2;

[0115] The oversize of the filter 8 of the dehydration filter device 2 is sucked off and sent to a testing laboratory to be tested for suitability for reimplantation of said portion T2a of said adipose tissue T1.

[0116] Of course, under actual use conditions, sending the portion T2a of the adipose tissue T1 to the laboratory would be replaced by its re-implantation into the same donor patient using known techniques and instruments.

[0117] Results

[0118] The following is a brief review of the results obtained, as shown in the graph of Figure 567 and in Tables T1 and T2, which describe the labeling and sampling points of the samples.

[0119] Form T1

[0120] Table 2

[0121]

[0122] Brief observation of sample samples collected and subsequently processed in syringes provided initial macroscopic evidence of homogeneity and elimination of blood and fibrous residues in the adipose tissue. Examination under a light microscope confirmed this initial observation.

[0123] Figure 5 The diagrams in show how the method of the invention is able not only to maintain the integrity and viability of the cells present in the collected adipose tissue, but also to increase their concentration through cell selection and reduction of liquid residues. The first step of the method of the invention (filtration using vibrating sieving) has been shown to be able to appropriately minimize cell stress in a step aimed at the restricted collection of the final product. By combining debridement and purification of the collected sample, a three-fold increase in the number of viable cells was successfully obtained at the end of this step. The second step of the method of the invention (dehydration filter) is an indispensable and fundamental part, because reducing the excess liquid not only does not compromise the performance of the first step, but on the contrary optimizes it, making the final data outstanding.

[0124] The diagram in Figure 67 shows how all cells express the markers sought in all samples examined and illustrates the synergy obtained with hyaluronic acid. The data are clearly and consistently reproducible, represented by predominantly mesenchymal cells identified by the markers CD73, CD90, CD29.

[0125] The absence of hematopoietic / macrophage stem cells demonstrates efficient elimination of blood remnants. Adipocyte markers are poorly expressed, as the selection of mesenchymal cells has been optimized while maintaining adipose support for the stromal vascular niche. The quality of the population is given by the almost absent response of CD45, which indicates an optimized elimination of mature cells.

[0126] Therefore, for mesenchymal (fibroblast-like) stem cells, the population must be considered highly homogeneous.

Claims

1. A method for treating adipose tissue, comprising the following steps: Collecting adipose tissue (T1) from a donor patient, characterized by comprising the following steps: providing a plurality of screens having progressively decreasing mesh sizes; Providing a radial agitator device of the oscillating type; Provide vacuum dehydration filter unit; The adipose tissue (T1) is subjected to a first physical-mechanical treatment step through a plurality of sieves with gradually decreasing mesh sizes, and at least one oversize fraction containing fiber fractions of different particle sizes and a remaining wet and homogenized undersize fraction are separated by the oscillating radial agitator device, wherein the undersize fraction is a nano-fat type rich in stem cells; At least the remaining wet and homogenized undersize fraction is subjected to a second physical treatment step by vacuum dehydration filter treatment to eliminate water in order to concentrate this fraction and obtain an adipose tissue (T2a) of nano-fat particle size, without fibrous and / or oily and / or blood residues (R1) and water residues (R2), containing a high concentration of viable mesenchymal stem cells, wherein the adipose tissue (T2a) is suitable for being intended for reimplantation into the same donor patient.

2. The method according to claim 1, characterized in that The physical and mechanical treatment step comprises the following steps: A filter column (1) is provided, which is provided with at least a first and a second screen (3, 4) placed in sequence, the mesh size of which decreases in the direction of the filtration flow; The filter column (1) is operated by means of a radial agitator device (5) of the oscillating type; The fat tissue (T1) is subjected to impurity separation and homogenization treatment by using a first sieve (3) to obtain an oversize material (SP) and a undersize material (ST); Disposal of fiber and / or oily and / or blood residues (R1) contained in the oversize (SP) of the first sieve 3; The undersize (ST) of the sieve immediately preceding it is subjected to impurity separation and homogenization treatment by means of a second sieve (4) to obtain an oversize fraction (T2b) and a wet undersize fraction (ST) comprising nano-fat tissue (T2a) and water residue (R2).

3. The method according to claim 1, characterized in that The physical treatment step comprises the following steps: Providing a vacuum dehydration filter device (2) provided with a filter (8); Providing a suction pump (9) suitable for operating the vacuum dehydration filter device (2); The remaining wet and homogenized undersize fraction (ST) is subjected to a water residue (R2) separation treatment by means of a filter (8) and a suction pump (9) of a vacuum dehydration filter device (2), thereby obtaining a fat tissue (T2a) with nano-fat particle size and no water residue (R2).

4. The method according to claim 2, characterized in that The following further steps are included: The oversize (SP) of the second sieve (4) is collected, comprising adipose tissue (T2b) of micro-fat particle size, naturally having a low water content and being free of fibrous and / or oily and / or blood residues (R1), containing a high concentration of viable mesenchymal stem cells, wherein the adipose tissue (T2b) of micro-fat particle size is suitable for being intended for re-implantation into the same donor patient.

5. The method according to claim 2, characterized in that The following further steps are included: providing a third screen (7) disposed between the first and second screens (3, 4) to obtain a further oversize (SP) and a further undersize (ST); Passing through a third screen (7) to separate the impurities and subsequently homogenize the undersize (ST) of the screen immediately preceding it; The oversize (SP) of the third sieve (7) is collected to obtain adipose tissue (T2c) with milli-fat particle size, naturally low in water content and free from fibrous and / or oily and / or blood residues (R1), containing a high concentration of viable mesenchymal stem cells, intended for reimplantation into the same donor patient.

6. A device for processing adipose tissue (T1) collected from a donor patient, characterized in that It includes: A filter column (1) provided with at least a first and a second screen (3, 4) whose mesh size decreases in the direction of the filtration flow, adapted to be operated by a radial agitator device (5) of the oscillating type; A vacuum dewatering filter device (2) provided with a filter (8) adapted to be operated by a suction pump (9), The filtration column (1) allows obtaining, through the second sieve (4), an undersize (T2a) of adipose tissue comprising wet nano-fat particles and a water residue (R2) and an oversize comprising adipose tissue (T2b) of micro-fat particles, naturally having a low moisture content, and the vacuum dehydration filter device (2) allows obtaining adipose tissue (T2a) without water residue (R2), wherein each adipose tissue (T2a), (T2b) is free of fibrous and / or oily and / or blood residues (R1), contains a high concentration of viable mesenchymal stem cells, and at least one of these adipose tissues is intended for re-implantation into the same donor patient.

7. The device according to claim 6, characterized in that The first and second screens (3, 4) comprise screen openings with sizes of 2 mm and 0.6 mm, respectively.

8. The device according to claim 6, characterized in that The filter column (1) comprises: a reversible closing cover (20) of the first screen (3) provided with a sealing point (21) for the insertion of adipose tissue (T1) which can be optionally combined with a therapeutic substance and / or dosed; The collecting chamber (6) for the undersize (ST) of the second screen (4) is equipped with a flat bottom or a slightly inclined bottom.

9. The device according to claim 6, characterized in that The filter column (1) also includes: The third screen (7) is arranged at an intermediate position between the first and second screens (3, 4), The third sieve (7) allows obtaining a further oversize comprising adipose tissue of milli-fat size (T2c), naturally having a low water content and without fibrous and / or oily and / or blood residues (R1), containing a high concentration of viable mesenchymal stem cells, intended for reimplantation into the same donor patient.

10. The device according to claim 9, characterized in that The third screen (7) defines the central part of the filter column (1).

11. The device according to claim 9, characterized in that The mesh size of the third sieve (7) is 1 mm.

12. The device according to claim 6, characterized in that The vacuum dehydration filter device (2) comprises: an upper part (10) provided with a reversible closing cover (22); an atmospheric air (A) inlet (11) located on the closing cover (22); a one-way filter valve device (23) associated with the inlet (11) and adapted to filter atmospheric air (A); A lower part (12) provided with a tank (13) for collecting the water residue (R2) separated from the undersize (ST) of the second screen (4) of the filter column (1).

13. The device according to claim 6, characterized in that The filter (8) of the vacuum dehydration filter device (2) is made of paper or steel and has a porosity between 20 and 25 microns.

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