Compositions for inhibiting the discoloration of ascorbyl glucoside and their use in antioxidant skin care products

Abstract

The application discloses a composition for inhibiting discoloration of ascorbyl glucoside and application of the composition in antioxidant skin care products, and belongs to the field of toiletry preparations. The composition for inhibiting discoloration of ascorbyl glucoside comprises the following components in mass fractions: ascorbyl glucoside 10-15 parts, pine mushroom extract 2-4 parts, Cymbidium goeringii root extract 2-5 parts and astaxanthin 1-3 parts. The composition provided by the application has good stability when ascorbyl glucoside, pine mushroom extract, Cymbidium goeringii root extract and astaxanthin are used in combination, effectively inhibits discoloration of ascorbyl glucoside over time, enhances the antioxidant performance of ascorbyl glucoside, and has important significance for effective application of ascorbyl glucoside in skin care products.

Description

Technical Field

[0001] The invention belongs to the field of cosmetic preparations, and particularly relates to a composition for inhibiting discoloration of ascorbyl glucoside and application of the composition in antioxidant skin care products. Background Art

[0002] In cosmetics, ascorbyl glucoside acts as a whitening antioxidant. Its primary mechanism of action is to inhibit tyrosinase activity in the skin, reducing the production of dopaquinone, thereby inhibiting melanin production, resulting in brightening the skin and reducing pigmentation. Furthermore, ascorbyl glucoside promotes collagen production, helping to reduce wrinkles and improve skin roughness, demonstrating significant anti-aging effects. It also effectively protects the skin from UV damage, reduces the production of free radicals, and protects the skin from oxidative damage.

[0003] However, despite the many advantages of ascorbyl glucoside, its color stability remains a problem that needs to be addressed in practical applications. Due to the influence of environmental factors (such as light, temperature, humidity, etc.), the color of ascorbyl glucoside may change, which not only affects its appearance but also its performance in cosmetics.

[0004] Therefore, developing a composition that can stabilize the color of ascorbyl glucoside is of great significance for improving its application effect in antioxidant skin care products. Summary of the Invention

[0005] To address the shortcomings of the prior art, the present invention aims to provide a composition for inhibiting the discoloration of ascorbyl glucoside and its use in antioxidant skincare products. The composition effectively inhibits the discoloration of ascorbyl glucoside over time, maintaining its good appearance. Furthermore, the composition further enhances the antioxidant properties of ascorbyl glucoside, enabling it to perform better in skincare products.

[0006] In order to achieve the above object, the present invention discloses the following technical solutions:

[0007] In a first aspect, the present invention provides a composition for inhibiting discoloration of ascorbyl glucoside, wherein the composition comprises the following components in parts by mass:

[0008]

[0009]

[0010] Preferably, the composition comprises the following components in parts by mass:

[0011]

[0012] In a second aspect, the present invention provides use of the composition described in the first aspect in preparing a skin care product with whitening and / or anti-oxidation effects.

[0013] In a third aspect, the present invention provides a whitening and antioxidant essence, wherein the essence comprises the composition for inhibiting discoloration of ascorbyl glucoside according to the first aspect.

[0014] Preferably, the composition is added to the essence in an amount of 0.5-1.0 wt %.

[0015] More preferably, the essence further comprises a moisturizer, a preservative, a pH adjuster, and a solvent.

[0016] More preferably, the moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol;

[0017] The preservative is selected from p-hydroxyacetophenone;

[0018] The pH regulator is selected from arginine;

[0019] The solvent is selected from deionized water.

[0020] In a fourth aspect, the present invention provides a method for preparing the whitening and antioxidant essence according to the third aspect, comprising the following steps:

[0021] S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm after stirring to obtain component A;

[0022] S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B;

[0023] S3. Cool component B to 40°C, add the composition for inhibiting discoloration of ascorbyl glucoside at a speed of 500 rpm, continue stirring and mixing for 10 minutes, and finally add a pH adjuster to adjust the pH to 6.5-7.0, stop stirring, and discharge the material to obtain the whitening antioxidant essence.

[0024] In the present invention:

[0025] Ascorbyl glucoside is a water-soluble vitamin C derivative with antioxidant activity similar to vitamin C, capable of scavenging free radicals and preventing oxidation reactions. While it is more stable than vitamin C, it is still susceptible to oxidation, leading to discoloration.

[0026] Matsuba extract has excellent antioxidant properties. It contains a variety of antioxidant compounds, including matsutake polysaccharides, polyphenols, and flavonoids. These ingredients can effectively scavenge free radicals and inhibit oxidation reactions. Furthermore, matsutake extract has certain antibacterial properties, which can further improve the stability of the overall formula.

[0027] Tiger orchid root extract is rich in various antioxidant ingredients, such as polyphenols and alkaloid compounds, which can effectively scavenge free radicals and protect cells from oxidative damage. At the same time, tiger orchid root extract also has certain antibacterial effects, which can further improve the stability of the overall formula.

[0028] Astaxanthin is a carotenoid with strong antioxidant activity. The conjugated double bonds in its structure can effectively scavenge free radicals and prevent the occurrence of oxidation reactions. In addition, astaxanthin is lipophilic and hydrophilic, and can scavenge free radicals from the cell surface and the inside of the phospholipid membrane, and more effectively penetrate into the skin to exert its antioxidant effect.

[0029] The present invention has found that ascorbyl glucoside, Tricholoma matsutake extract, Cyperus tigrinum root extract and astaxanthin all have strong antioxidant activity, and they can work together to inhibit the occurrence of oxidation reactions and improve the stability of the formula.

[0030] Beneficial effects of the present invention:

[0031] 1. The composition provided by the present invention has good stability when used in combination with ascorbyl glucoside, Tricholoma matsutake extract, Cyperus tigrinum root extract, and astaxanthin, effectively inhibits the discoloration of ascorbyl glucoside over time, and enhances the antioxidant properties of ascorbyl glucoside;

[0032] 2. The whitening antioxidant essence provided by the present invention has excellent antioxidant properties and tyrosinase activity inhibition properties, which can effectively reduce the production of melanin, thereby achieving a whitening effect. DETAILED DESCRIPTION

[0033] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0034] Unless otherwise specified, the test methods used in the specific embodiments are all conventional methods; the materials, reagents, etc. used are all commercially available unless otherwise specified; the percentages mentioned in the specific embodiments are all mass percentages unless otherwise specified.

[0035] In the present invention:

[0036] The Tricholoma matsutake extract is a Tricholoma matsutake extract, INCI name TRICHOLOMA MATSUTAKE EXTRACT, purchased from Yunnan Yingge Biotechnology Co., Ltd., specification 10:1;

[0037] Cymbidium grandiflorum root extract is Cymbidium grandiflorum root extract, INCI name is CYMBIDIUM GRANDIFLORUM ROOT EXTRACT, purchased from Yunnan Yingge Biotechnology Co., Ltd., specification 20:1;

[0038] Astaxanthin: INCI name ASTAXANTHIN, water-soluble astaxanthin prepared according to the preparation method of Example 1 in Chinese patent CN111793014B;

[0039] Ascorbyl glucoside: INCI name ASCORBYL GLUCOSIDE, purchased from Cosfa International Trading (Guangzhou) Co., Ltd.

[0040] Other raw materials are commercially available.

[0041] Preparation of the composition:

[0042] The raw materials were accurately weighed according to the mass ratio in Table 1, and the weighed raw materials were placed in a mixer and mixed evenly to obtain a composition for inhibiting the discoloration of ascorbyl glucoside.

[0043] Table 1 Raw materials and their mass fractions of the composition

[0044] Raw materials\group Composition 1 Composition 2 Composition 3 Composition 4 Composition 5 Composition 6 Composition 7 Composition 8 Ascorbyl Glucoside 10 13 15 13 13 13 13 21 Tricholoma matsutake extract 2 3 4 - 4.8 4 - - Tiger orchid root extract 2 3 5 4.8 - 4 - - Astaxanthin 1 2 3 3.2 3.2 - 8 -

[0045] Note: “-” in the table means no additive.

[0046] Performance testing:

[0047] 1. Stability test

[0048] The compositions 1 to 8 were diluted with a solvent to a concentration of 1 wt % of a test sample, and the following tests were performed, wherein the solvent was deionized water.

[0049] High temperature test: The constant temperature incubator was adjusted to 45±1°C. The test samples prepared above were placed in three transparent glass bottles with a sample volume of 20 mL per bottle. After sealing, the bottles were placed in a constant temperature incubator. After 3 months, the bottles were taken out and returned to room temperature. The changes in appearance were observed. The test results are shown in Table 2.

[0050] Room temperature test: The test samples prepared above were placed in three transparent glass bottles, with a sample volume of 20 mL per bottle. After sealing, they were placed at room temperature for 3 months, and the changes in the appearance of the samples were observed. The test results are shown in Table 2.

[0051] Lighting test: A lighting device was installed 20-30 cm above the laboratory bench. The lighting conditions were set to LED light (light color 6000K) and illuminance (300 lx) to meet the test requirements. The test samples prepared above were placed in three transparent glass bottles, each containing 20 mL. After sealing, the bottles were placed under the specified lighting conditions. After three months, the bottles were removed, returned to room temperature, and their appearance was observed for changes. The test results are shown in Table 2.

[0052] Table 2 Stability test results

[0053] Group\Test Item 45±1℃, place for 3 months Tested at room temperature, placed for 3 months Light test, placed for 3 months Test results Composition 1 constant constant constant Stablize Composition 2 constant constant constant Stablize Composition 3 constant constant constant Stablize Composition 4 Darkening Darkening Darkening Unstable Composition 5 Darkening Darkening Darkening Unstable Composition 6 Darkening Darkening Darkening Unstable Composition 7 Darkening Darkening Darkening Unstable Composition 8 Darkening Darkening Darkening Unstable

[0054] Note: The test sample is a colorless to orange transparent solution before being placed. If the color turns yellow or darker after being placed, it is unstable.

[0055] Result analysis:

[0056] It can be seen from the test results in Table 2 that under different temperature tests, the colors of the five groups of compositions 4-8 changed, while the colors of the three groups of compositions 1-3 did not change, and the properties of compositions 1-3 were more stable than those of compositions 4-8, proving that ascorbyl glucoside, Tricholoma matsutake extract, Tiger orchid root extract and astaxanthin are compounded in the ratio provided by the present invention. When used, they have good stability. After being placed at 45±1°C for 3 months, at room temperature, and under light for 3 months, the color and appearance of the composition for inhibiting the discoloration of ascorbyl glucoside provided by the present invention did not change. However, when the composition lacked any one or more of Tricholoma matsutake extract, Tiger orchid root extract and astaxanthin, the stability of the composition deteriorated, further proving that the composition has a significant effect on inhibiting the discoloration of ascorbyl glucoside.

[0057] 2. Antioxidant performance test

[0058] Before placement: Take a solvent to dilute the compositions 1-8 that have not been tested at room temperature to a concentration of 0.0001 wt % of the test sample, and perform the following tests, wherein the solvent is deionized water.

[0059] After storage: Compositions 1-8 after being stored at room temperature for 3 months were further diluted with a solvent to a concentration of 0.0001 wt % of the test sample, and the following tests were performed, wherein the solvent was deionized water.

[0060] 2.1 Determination of DPPH free radical scavenging ability:

[0061] DPPH was prepared with anhydrous ethanol to prepare 2×10 -4 For a 1.5 mol / L DPPH solution, prepare several 2 mL portions of test sample, DPPH solution, and anhydrous ethanol. Mix 2 mL of test sample and 2 mL of DPPH solution, incubate at room temperature for 30 minutes, and measure the absorbance at 517 nm to obtain Ai. Mix 2 mL of test sample and 2 mL of anhydrous ethanol, then measure the absorbance as described above to obtain Aj. Mix 2 mL of DPPH solution and 2 mL of anhydrous ethanol, then measure the absorbance as described above to obtain Ac. Perform three replicate measurements for each sample, and calculate the average value.

[0062] The scavenging rate of DPPH free radicals for each test sample was calculated according to the following formula (1) and recorded in Table 3 below.

[0063] Clearance (%) = (1-(Ai-Aj) / Ac) × 100% (1)

[0064] Where: Ai is the absorbance of a mixture of 2 mL of test sample and 2 mL of DPPH solution; Aj is the absorbance of a mixture of 2 mL of test sample and 2 mL of anhydrous ethanol; Ac is the absorbance of a mixture of 2 mL of DPPH solution and 2 mL of anhydrous ethanol.

[0065] 2.2 Determination of superoxide free radical O2- scavenging ability:

[0066] Test sample absorbance test: Accurately aspirate 1 mL of test sample, add 4.5 mL of 50 mmol / L Tris-HCl buffer (pH = 8.2), then mix well. Incubate in a 25°C constant temperature water bath for 20 minutes, then take out and immediately add 0.3 mL of 25 mmol / L pyrogallol solution. After rapid shaking, react in a 25°C constant temperature water bath for 5 minutes. Then add 1 mL of 8 mmol / L HCl, mix well, and terminate the reaction. Measure the absorbance at 325 nm.

[0067] Absorbance test of blank control group: perform the above operation using distilled water instead of the test sample.

[0068] Each group of samples was measured three times in parallel and the average value was taken.

[0069] The clearance rate was calculated according to formula (2) and recorded in Table 3 below.

[0070] Clearance (%) = (A0-A1) / A0×100% (2)

[0071] Where: A0 is the average absorbance of the blank; A1 is the average absorbance of the test sample.

[0072] 2.3 Determination of hydroxyl radical scavenging ability:

[0073] 1 mL of 0.15 mmol / L FeSO4, 0.4 mL of 2 mmol / L salicylic acid, 0.2 mL of the test sample, 1 mL of 6 mmol / L H2O2, and 0.4 mL of distilled water were added to a test tube to prepare a sample as the test sample group; distilled water was used instead of the aforementioned test sample to prepare a sample as the blank group; distilled water was used instead of the aforementioned salicylic acid to prepare a sample as the control group; the reaction was carried out at 37°C for 30 minutes; each sample was measured in parallel 3 times, and the average value was taken.

[0074] The hydroxyl radical scavenging capacity was calculated according to formula (3) and recorded in Table 3 below.

[0075] Clearance (%) = (A 空白 -(A 样品 -A 对照 )) / A 空白 ×100%........(3)

[0076] Table 3 Antioxidant performance test results

[0077]

[0078]

[0079] Result analysis:

[0080] According to the test results in Table 3, after storage, the antioxidant properties of the five groups of compositions 4-8 showed a more obvious decline, while the antioxidant properties of the three groups of compositions 1-3 only experienced a relatively weak decline, indicating that the ascorbyl glucoside, Tricholoma matsutake extract, Cyperus tigrinum root extract and astaxanthin of the present invention have good stability when used in combination. When any one or more of Tricholoma matsutake extract, Cyperus tigrinum root extract and astaxanthin are missing, the antioxidant properties of the composition will deteriorate over time.

[0081] Preparation of application examples:

[0082] Weigh the raw materials according to Table 4;

[0083] S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm after stirring to obtain component A;

[0084] S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B;

[0085] S3. Component B was cooled to 40°C, and the composition was added at a speed of 500 rpm. The mixture was stirred and mixed for 10 minutes. Finally, a pH adjuster was added to adjust the pH to 6.5. The stirring was stopped and the mixture was discharged to obtain the essence.

[0086] Table 4 Mass percentage of raw materials in application examples

[0087]

[0088] Note: “-” in the table means no additive.

[0089] Application example whitening effect test:

[0090] Test environment conditions: In a stable indoor environment, the indoor temperature is 24℃-27℃ and the relative humidity is 40%-50%.

[0091] Tested substances: Essences from Application Examples 1-3. All essences had been sealed and left to stand at room temperature for 3 months before use.

[0092] Subjects: Volunteers aged 30±2 years with similar skin color ITA values ​​were selected.

[0093] Experimental plan: Each test substance was tested on 5 volunteers, with the test site being the face. The multifunctional skin tester (model: MPA580) manufactured by Courage Khazaka, Germany, was used to measure the initial ITA value of each group of volunteers. The test substances of Application Examples 1 to 3 were then used, 10 ml at a time, twice a day for 4 weeks. The ITA value of each group of volunteers was measured (the average value was taken) to evaluate the whitening effect of the test substance. A higher ITA value indicates a better whitening effect. The test results are shown in Table 5 below.

[0094] Table 5 ITA value test results of application examples 1-3

[0095]

[0096] Result analysis:

[0097] According to the results in Table 5, the whitening effects of the test substances in Application Examples 1-3 show a certain positive correlation with the concentration of Composition 2. The higher the concentration of Composition 2, the better the whitening effect of the essence, which has a certain concentration dependence. At the same time, by comparing Application Examples 1-3, it can be seen that the test substance in Application Example 3 has obviously better whitening effect, which further proves that the composition of ascorbyl glucoside, Tricholoma matsutake extract, Cynanchum root extract and astaxanthin provided by the present invention can maintain the stability of ascorbyl glucoside well when used in combination in this ratio, so that the product maintains good skin care effect.

[0098] The relationship between tyrosinase and skin whitening is primarily reflected in the synthesis of melanin. Tyrosinase is a key enzyme in melanin synthesis, catalyzing the conversion of tyrosine into dopa, which is then further oxidized to form dopaquinone, ultimately forming melanin. Melanin formation is the primary cause of skin darkening. Therefore, the composition of ascorbyl glucoside, Tricholoma matsutake extract, Cyperus tigrinus root extract, and astaxanthin provided by the present invention exhibits excellent antioxidant and tyrosinase inhibitory properties, effectively reducing melanin production and achieving a whitening effect.

[0099] Finally, it should be noted that the above embodiments and comparative examples are only used to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for inhibiting discoloration of ascorbyl glucoside, characterized in that: The composition comprises the following components in parts by mass:

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by mass:

3. Use of the composition according to claim 1 or 2 in the preparation of a skin care product with whitening and / or anti-oxidation effects.

4. A whitening and antioxidant essence, characterized in that: The essence contains the composition for inhibiting discoloration of ascorbyl glucoside according to claim 1 or 2.

5. The whitening and antioxidant essence according to claim 4, characterized in that: The composition is added to the essence in an amount of 0.5-1.0 wt %.

6. The whitening and antioxidant essence according to claim 4 or 5, characterized in that: The essence also contains a moisturizer, a preservative, a pH regulator and a solvent.

7. The whitening and antioxidant essence according to claim 6, characterized in that: The moisturizing agent is selected from at least one of glycerin, allantoin, 1,3-propylene glycol, butylene glycol, and 1,2-hexanediol; The preservative is selected from p-hydroxyacetophenone; The pH regulator is selected from arginine; The solvent is selected from deionized water.

8. The method for preparing the whitening and antioxidant essence according to claim 7, characterized in that: The following steps are involved: S1. Mix the moisturizer and deionized water, heat to 55°C, stir at 200 rpm for 20 min, and keep warm after stirring to obtain component A; S2. The preservative and component A were mixed at 55°C and stirred at 200 rpm for 5 min to obtain component B; S3. Cool component B to 40°C, add the composition for inhibiting discoloration of ascorbyl glucoside at a speed of 500 rpm, continue stirring and mixing for 10 minutes, and finally add a pH adjuster to adjust the pH to 6.5-7.0, stop stirring, and discharge the material to obtain the whitening antioxidant essence.

Images