Magnetic separation device

Abstract

The invention provides a magnetic separation device. The magnetic separation device comprises a driving mechanism, a magnetic mechanism and a separation mechanism. The driving mechanism comprises a driving unit and a transmission unit, the magnetic mechanism comprises a magnetic part, and the sorting mechanism comprises a sorting part. According to the magnetic separation device, the magnetic part moves under the action of the driving unit and the transmission unit, so that the separation part is located in the magnetic field range of the magnetic part or located outside the magnetic field range of the magnetic part, the magnetic field can be loaded or eliminated for the separation part, and automation of magnetic field loading and magnetic field removal is achieved. Due to the fact that the mechanical structure of the magnetic separation device is determined, loading and eliminating of the magnetic field each time have high consistency, and therefore the good consistency can be kept in the separation process of the target object.

Description

Technical Field

[0001] The present application relates to the field of separation technology, and in particular to a magnetic separation device. Background Art

[0002] Magnetic materials (e.g., magnetic beads, which are micron-sized iron beads) can be specifically bound to targets (e.g., target cells such as T cells, nucleic acid molecules, etc.) through biotechnology. Utilizing the magnetism of magnetically labeled targets and using magnetic sorting methods to sort out highly specific targets is a key step in modern medical and scientific research activities.

[0003] In order to screen out the target (for example, T cells), the antigen can be specifically adsorbed on the surface of the target, and the antibody matching the antigen can be coated (coating refers to the process of binding the antigen or antibody to the surface of the solid phase carrier) on the surface of the magnetic material. When the target bound to the antigen passes through the magnetic material coated with the antibody, the antibody binds to the antigen to form a target with magnetic material. When the target with magnetic material flows through the magnetic field, it will stay and be adsorbed in the magnetic field under the action of magnetic force, while other impurities without magnetic material will flow through the magnetic field. After removing the magnetic field, the target with magnetic material can be eluted under the flushing of the eluent, and the collected eluent will only contain the desired target.

[0004] However, existing magnetic separation devices mostly rely on manual loading and removal of the magnetic field, which is inefficient, and manual operation leads to poor consistency in separation results. Summary of the invention

[0005] In view of this, the present application proposes a magnetic separation device to solve at least one of the above problems.

[0006] One embodiment of the present application provides a magnetic separation device, which includes a driving mechanism, a magnetic mechanism and a separation mechanism.

[0007] The driving mechanism includes a first mounting seat, and a driving unit and a transmission unit arranged on the first mounting seat. The driving unit is used to generate power and drive the transmission unit to move, and the transmission unit is used to transmit the power.

[0008] The magnetic mechanism comprises a magnetic member, and the magnetic member is arranged on the transmission unit and can move under the drive of the transmission unit.

[0009] The sorting mechanism includes a mounting cover and a sorting member disposed on the mounting cover. The mounting cover includes a cavity, the opening of which faces the magnetic mechanism. The top wall of the cavity facing away from the magnetic mechanism is recessed toward the opening of the cavity to form a receiving groove, and the sorting member is disposed in the receiving groove.

[0010] The magnetic separation device has a first state and a second state. When the magnetic separation device is in the first state, the magnetic component is located in the cavity, and the separation component is located within the magnetic field range of the magnetic component. When the magnetic separation device is in the second state, the magnetic component is outside the cavity, and the separation component is located outside the magnetic field range of the magnetic component.

[0011] In one embodiment, the magnetic mechanism further includes a second mounting seat, the magnetic member is disposed on the second mounting seat, and the second mounting seat is fixed to the transmission unit.

[0012] In one embodiment, the magnetic mechanism further comprises a magnetic seat fixed on the second mounting seat. The magnetic seat comprises a bottom plate and two side plates arranged opposite to each other and extending in the same direction from the edge of the bottom plate. The edge of each side plate extends toward the other side plate to form an extension plate, and the extension plate, the side plate and the bottom plate together form a groove. The surface of each side plate facing the other side plate is recessed in a direction away from the other side plate to form a receiving groove, and the magnetic member is arranged in the receiving groove.

[0013] In one embodiment, the edge of the extension plate extends toward the direction close to the bottom plate to form a protrusion.

[0014] In one embodiment, an iron plate is further provided in the receiving groove, and the iron plate is located between the magnetic member and the side plate.

[0015] In one embodiment, a plurality of magnetic members are disposed in the receiving groove.

[0016] In one embodiment, the separation element comprises a main body and extension rods arranged at both ends of the main body. Filter plates are arranged at both ends of the main body, and a plurality of magnetic particles are arranged in the main body, and the magnetic particles are located between the filter plates.

[0017] In one embodiment, the sorting mechanism further comprises a fixed seat, which is arranged in the accommodating groove. The fixed seat comprises a fixed plate and a bearing plate arranged at both ends of the fixed plate, and the bearing plate is provided with a through groove penetrating the bearing plate along the thickness direction. The extension rod extends from the through groove, and the main body is clamped between the bearing plates.

[0018] In one embodiment, the driving unit includes a rotating shaft. The transmission unit includes a gear and a rack meshing with the gear, and the gear is sleeved on the rotating shaft. The rack is disposed on the first mounting seat and is configured to be movable on the first mounting seat.

[0019] In one embodiment, the transmission unit further comprises a slide rail and a slider disposed on the first mounting seat, wherein the slider is configured to slide on the slide rail. The second mounting seat is fixed on the rack and the slider.

[0020] In the magnetic separation device of the present application, the magnetic part moves under the action of the driving unit and the transmission unit, so that the separation part is located within the magnetic field range of the magnetic part or outside the magnetic field range of the magnetic part, so that the magnetic field can be loaded or eliminated on the separation part, realizing the automation of loading and removing the magnetic field. Since the mechanical structure of the magnetic separation device is fixed, each magnetic field loading and elimination has a strong consistency, so the separation process of the target object can maintain good consistency. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A schematic structural diagram of a magnetic separation device provided in one embodiment of the present application.

[0022] Figure 2 for Figure 1 Schematic diagram of the exploded structure of the magnetic separation device shown.

[0023] Figure 3 for Figure 1 The shown diagram is a schematic diagram of the magnetic mechanism and separation elements of the magnetic separation device in the first state.

[0024] Figure 4 for Figure 1 The shown diagram is a schematic diagram of the magnetic mechanism and separation elements of the magnetic separation device in the second state.

[0025] Figure 5 for Figure 2 The structure diagram of the magnetic base and magnetic parts of the magnetic separation device in another embodiment is shown.

[0026] Figure 6 for Figure 2 The structure diagram of the magnetic base and magnetic parts of the magnetic separation device in another embodiment is shown.

[0027] Figure 7 for Figure 2 The structure diagram of the magnetic seat and magnetic parts of the magnetic separation device in one embodiment is shown.

[0028] Figure 8 for Figure 7 A top view of the magnetic base and magnetic member is shown.

[0029] Fig. 9 for Figure 2 The structure diagram of the magnetic base and magnetic parts of the magnetic separation device in another embodiment is shown.

[0030] Fig.10 for Figure 2 The structure diagram of the magnetic component of the magnetic separation device in another embodiment is shown.

[0031] Fig.11 for Figure 2 A cross-sectional view of a separation component of a magnetic separation device is shown.

[0032] Fig.12 for Figure 2 A schematic structural diagram of the mounting cover of the magnetic separation device shown.

[0033] Fig.13 for Figure 2 The structural schematic diagram of the fixed seat of the magnetic separation device is shown.

[0034] Main component symbols

[0035] Magnetic separation device 100

[0036] Driving mechanism 10

[0037] Magnetic mechanism 20

[0038] Sorting mechanism 30

[0039] First mounting seat 11

[0040] Drive unit 12

[0041] Transmission unit 13

[0042] Sensor 14

[0043] Sensor sheet 15

[0044] Rotating shaft 121

[0045] Through hole 110

[0046] Gear 131

[0047] Rack 132

[0048] Slide 133

[0049] Slider 134

[0050] Second mounting seat 21

[0051] Magnetic seat 22

[0052] Magnetic parts 23

[0053] Iron Plate 24

[0054] Horizontal plate 211

[0055] Vertical plate 212

[0056] Grooves 220, 230

[0057] Storage tank 221

[0058] Bottom plate 222

[0059] Side Panel 223

[0060] Extension plate 224

[0061] Raised 225

[0062] Bending portion 231

[0063] Straight part 232

[0064] Mounting hood 31

[0065] Selection 32

[0066] Magnetic particles 33

[0067] Fixed seat 34

[0068] Cavity 310

[0069] Accommodating groove 311

[0070] Top wall 312

[0071] Extension wall 313

[0072] Projection 314

[0073] Main body 321

[0074] Extension rod 322

[0075] Filter plate 323

[0076] Fixed plate 341

[0077] Loading plate 342

[0078] Through groove 343

[0079] The following specific implementation manner will further illustrate the embodiments of the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0080] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the technical field of the present application. The terms used herein are only for the purpose of describing specific implementation methods and are not intended to limit the present application embodiments.

[0081] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0082] It will be understood that when a layer is referred to as being “on” another layer, it can be directly on the other layer or intervening layers may be present therebetween. In contrast, when a layer is referred to as being “directly on” another layer, there are no intervening layers present.

[0083] Some embodiments of the present application are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0084] See also Figure 1 and Figure 2 The present application provides a magnetic separation device 100 for separating a target object from a sample. The target object may be, but is not limited to, a cell, a nucleic acid molecule, etc. The magnetic separation device 100 includes a driving mechanism 10, a magnetic mechanism 20, and a separation mechanism 30.

[0085] The driving mechanism 10 includes a first mounting seat 11 and a driving unit 12 and a transmission unit 13 disposed on the first mounting seat 11. The driving unit 12 is used to generate power and drive the transmission unit 13 to move. The first mounting seat 11 is generally in the shape of a plate, and has a through hole 110 that penetrates the first mounting seat 11 along the thickness direction. The driving unit 12 can be fixed to the first mounting seat 11 by means of, but not limited to, a hexagon socket head screw, and can extend from the through hole 110. The transmission unit 13 is located on the surface of the first mounting seat 11 and is disposed near the through hole 110. The transmission unit 13 is used to transmit the power generated by the driving unit 12.

[0086] The magnetic mechanism 20 includes a magnetic member 23, which is disposed on the transmission unit 13 and can move under the drive of the transmission unit 13. The magnetic member 23 can be, but is not limited to, a magnet.

[0087] The sorting mechanism 30 includes a mounting cover 31 and a sorting member 32 disposed on the mounting cover 31. The mounting cover 31 is a hollow structure having a cavity 310, the opening of which faces the magnetic mechanism 20. The top wall of the cavity 310 facing away from the magnetic mechanism 20 is recessed toward the opening of the cavity 310 to form a receiving groove 311, and the sorting member 32 is disposed in the receiving groove 311.

[0088] like Figure 3As shown, the magnetic separation device 100 has a first state. When the magnetic separation device 100 is in the first state, the magnetic member 23 is inserted into the cavity 310, so that the separation member 32 is located within the magnetic field range of the magnetic member 23. At this time, the separation member 32 is loaded with a magnetic field, and the target object with magnetic particles (not shown) will be adsorbed on the surface of the separation member 32, and other impurities will flow away.

[0089] like Figure 4 As shown, the magnetic separation device 100 has a second state. When the magnetic separation device 100 is in the second state, the magnetic member 23 is located outside the cavity 310, and the separation member 32 is located outside the magnetic field range of the magnetic member 23. At this time, the separation member 32 removes the magnetic field, and the adsorbed target will be eluted under the flushing of the eluent, so that the desired target can be obtained from the collected eluent.

[0090] like Figure 1 and Figure 2 As shown, in some embodiments, the drive unit 12 includes a rotating shaft 121. In this embodiment, the drive unit 12 is a reducer motor (also referred to as a gear motor or a gear motor). In other embodiments, the reducer motor can also be replaced by other devices that can generate power. The transmission unit 13 includes a gear 131 and a rack 132 meshing with the gear 131. The rack 132 is roughly in the shape of a long strip, and one side surface thereof is provided with teeth meshing with the gear 131. The gear 131 is sleeved on the rotating shaft 121, and the rack 132 is arranged on the first mounting seat 11 and can move on the first mounting seat 11. The first mounting seat 11 can be fixed to the housing (not shown) of the magnetic separation device 100. Through the meshing of the gear 131 and the rack 132, the rotation of the drive unit 12 can be converted into the linear motion of the rack 132.

[0091] like Figure 1 and Figure 2 As shown, in some embodiments, the transmission unit 13 further includes a slide rail 133 and a slider 134 provided on the first mounting seat 11, and the slider 134 can slide on the slide rail 133. The slide rail 133 and the rack 132 are located on the same surface of the first mounting seat 11, and the extension direction of the slide rail 133 is parallel to the extension direction of the rack 132. The slide rail 133 can be fixed to the first mounting seat 11 by means of, but not limited to, hexagon socket head screws.

[0092] Furthermore, the magnetic mechanism 20 includes a second mounting seat 21. The magnetic member 23 is disposed on the second mounting seat 21, and the second mounting seat 21 can be fixed to the transmission unit 13 by means of, but not limited to, hexagon socket head screws.

[0093] like Figure 2As shown, in some embodiments, the second mounting seat 21 includes a horizontal plate 211 and a vertical plate 212, and the vertical plate 212 is substantially perpendicular to the horizontal plate 211. The horizontal plate 211 can be fixed to the surface of the rack 132 and the slider 134 away from the first mounting seat 11 by means of, but not limited to, hexagon socket head screws. The slide rail 133 and the slider 134 are used to make the magnetic mechanism 20 move linearly on the first mounting seat 11.

[0094] like Figure 2 and Figure 5 As shown, in some embodiments, the magnetic mechanism 20 also includes a magnetic base 22 fixed on the second mounting base 21, the cross-section of the magnetic base 22 is roughly U-shaped, and the material of the magnetic base 22 can be aluminum. The magnetic base 22 includes a bottom plate 222 and two side plates 223 that are oppositely arranged and extend in the same direction from the edge of the bottom plate 222. The edge of each side plate 223 extends toward the other side plate 223 to form an extension plate 224, that is, there are two extension plates 224. The side plate 223 is roughly perpendicular to the bottom plate 222, and the extension plate 224 is roughly perpendicular to the side plate 223. The bottom plate 222 can be fixed to the vertical plate 212 of the second mounting base 21 by means of, but not limited to, hexagon socket head screws, and the bottom plate 222 is roughly parallel to the vertical plate 212.

[0095] Two extension plates 224, two side plates 223 and bottom plate 222 are enclosed to form a groove 220, and the bottom wall of the groove 220 is the bottom plate 222. The two extension plates 224 are not connected to each other, forming the opening of the groove 220. The side wall of the groove 220 is the inner surface of the side plate 223 (i.e., the surface facing the other side plate 223). The surface of each side plate 223 facing the other side plate 223 is recessed in a direction away from the other side plate 223 to form a receiving groove 221. That is, in this embodiment, there are two receiving grooves 221. The two receiving grooves 221 are arranged opposite to each other along their depth direction, wherein the opening of one receiving groove 221 faces the other receiving groove 221, and the depth direction of the receiving groove 221 is approximately perpendicular to the depth direction of the groove 220. The magnetic member 23 is arranged in the receiving groove 221, and the magnetic members 23 in the two receiving grooves 221 are arranged opposite to each other.

[0096] like Figure 5 As shown, in some embodiments, the number of magnetic members 23 in each receiving slot 221 is multiple, and the magnetic member 23 is roughly rectangular. In this embodiment, the number of magnetic members 23 in each receiving slot 221 is four, and in other embodiments, it can also be adjusted to three, five, six, etc. In other embodiments, such as Figure 6As shown, the number of magnetic parts 23 in each receiving slot 221 can also be only one, and one magnetic part 23 fills the receiving slot 221. At this time, the volume of the magnetic part 23 is relatively large, the middle part is magnetized and saturated (reaching the limit of the selected magnetic material), and the magnetic force of other parts is relatively weak. Compared with the method of setting a whole magnetic part 23 in each receiving slot 221, the method of setting multiple magnetic parts 23 in each receiving slot 221 can ensure that each magnetic part 23 is magnetized and saturated, and the magnetic force combined together is strong and balanced.

[0097] like Figure 7 and Figure 8 As shown, in some embodiments, the edge of the extension plate 224 extends toward the direction close to the bottom plate 222 to form a protrusion 225, and the material of the protrusion 225 can be aluminum. The two poles of the magnetic member 23 have a relatively large suction force ( Figure 8 The direction of the arrow in the middle indicates the direction of the magnetic force), and the protrusion 225 can limit the magnetic member 23 to prevent the two relative magnetic members 23 from being attracted to each other and causing safety accidents. Figure 8 As shown, specifically, when the magnetic seat 22 has a protrusion 225, the shape of the magnetic member 23 can be adapted to the shape formed by the receiving groove 221 and the protrusion 225, and its cross-sectional shape is roughly a "convex" shape.

[0098] Figure 7 The figure shows a case where a plurality of magnetic members 23 are arranged in each receiving groove 221. In this way, it can be ensured that each magnetic member 23 is saturated with magnetism, and the magnetic force when combined together is strong and balanced. Fig. 9 As shown, only one magnetic member 23 may be disposed in each receiving slot 221. One or more magnetic members 23 may be disposed in the receiving slot 221 and may be flexibly configured according to different application scenarios.

[0099] like Figures 5 to 9 As shown, in some embodiments, an iron plate 24 is further provided in the receiving groove 221, and the iron plate 24 is located between the magnetic member 23 and the side plate 223. More specifically, the iron plate 24 is located between the magnetic member 23 and the bottom wall of the receiving groove 221. The iron plate can exclude magnetism and enhance the magnetic field of the sorting member 32. In some embodiments, the iron plate 24 can also be omitted, and the magnetic member 23 can directly contact the bottom wall of the receiving groove 221.

[0100] like Figures 5 to 9As shown, in some embodiments, the surface of the bottom wall of the magnetic member 23 facing away from the receiving groove 221 can be flush with the surface of the extension plate 224 facing away from the side plate 223. That is, the magnetic member 23 will neither protrude from the receiving groove 221 nor leave excess space in the receiving groove 221, and the receiving groove 221 is just filled with the magnetic member 23 (iron plate 24). In this way, it is convenient to enclose the sorting member 32 in the groove 220 of the magnetic seat 22, so that the sorting member 32 is located within the magnetic field range of the magnetic member 23 and the magnetic field loaded on the sorting member 32 is relatively strong.

[0101] like Fig.10 As shown, in some embodiments, the magnetic mechanism 20 only includes a second mounting seat 21 and a magnetic member 23, and the magnetic member 23 is a U-shaped magnet. The U-shaped magnet includes a curved portion 231 and straight portions 232 respectively connected to the two ends of the curved portion 231, and the two straight portions 232 are arranged opposite to each other. The two straight portions 232 and the curved portion 231 are surrounded to form a groove 230. In the groove 230, the magnetic field direction of the U-shaped magnet is directed from one of the straight portions 232 to the other straight portion 232. The curved portion 231 can be fixed to the vertical plate 212 of the second mounting seat 21 by means of, but not limited to, a hexagon socket head screw (see Figure 2 ). The second mounting seat 21 moves driven by the rack 132 and the slider 134, thereby driving the U-shaped magnet to move, and then the sorting member 32 can be located in the groove 230, so that the sorting member 32 is loaded with a magnetic field. In some other embodiments, the U-shaped magnet can also be fixed to the second mounting seat 21 through a magnetic seat (not shown), that is, the magnetic mechanism 20 includes the second mounting seat 21, a magnetic seat (not shown) and a U-shaped magnet. The shape of the magnetic seat is compatible with the shape of the U-shaped magnet, so that the U-shaped magnet can be fixed to the magnetic seat.

[0102] like Fig.11 As shown, in some embodiments, the sorting member 32 includes a main body 321 and extension rods 322 provided at both ends of the main body 321. In this embodiment, the main body 321 is columnar. In other embodiments, the main body 321 may be in the shape of a cuboid, a cube, etc., which is not limited in this application. Fig.11 In the up and down direction in the main body 321), filter plates 323 are respectively provided at both ends of the main body 321. A plurality of magnetic particles 33 are provided in the main body 321, and the plurality of magnetic particles 33 are located between the two filter plates 323. The filter plate 323 can protect the magnetic particles 33 from penetration, while the target object can penetrate. The plurality of magnetic particles 33 can increase the adsorption area and amplify the magnetic field. The magnetic particles 33 may be iron beads with a diameter of 0.5 mm. In some embodiments, the surface of the magnetic particles may be coated with a hydrophilic coating as a protective layer (not shown) to prevent the magnetic particles 33 from scratching the target object. The material of the protective layer may be but is not limited to dextran.

[0103] like Fig.12 As shown, in some embodiments, the mounting cover 31 is generally a hollow cubic structure, which has a top wall 312 and four extension walls 313 extending from the edge of the top wall 312 in the same direction. The top wall 312 and the four extension walls 313 enclose a cavity 310, and the receiving groove 311 is formed by the outer surface of the top wall 312 being recessed inward, and the depth direction of the receiving groove 311 is parallel to the extension direction of the extension wall 313. It can be understood that when the outer surface of the top wall 312 is recessed inward to form the receiving groove 311, a protrusion 314 corresponding to the receiving groove 311 is formed in the cavity 310, and the protrusion 314 divides the cavity 310 into two parts. In the first state, the protrusion 314 is located in the groove 220 of the magnetic seat 22 (or in the groove 230 of the U-shaped magnet), and the magnetic member 23 is located on both sides of the protrusion 314, and is respectively located in the two parts of the cavity 310. The mounting cover 31 can be fixed on a housing (not shown) of the magnetic separation device 100 .

[0104] like Figure 2 and Fig.13 As shown, in some embodiments, the sorting mechanism 30 further includes a fixed seat 34, which can be clamped in the receiving groove 311 of the mounting cover 31. The fixed seat 34 includes a fixed plate 341 and a bearing plate 342 provided at both ends of the fixed plate 341, and the two bearing plates 342 are substantially perpendicular to the fixed plate 341. The fixed plate 341 can be abutted against the bottom wall of the receiving groove 311 (the bottom wall of the receiving groove 311 is parallel to the top wall 312), and the bearing plate 342 can be abutted against the side wall of the receiving groove 311 (the side wall of the receiving groove 311 is parallel to the extension wall 313). Each bearing plate 342 is provided with a through groove 343 that penetrates the bearing plate 342 along the thickness direction. Along the extension direction of the bearing plate 342 (perpendicular to the direction of the fixed plate 341), the through groove 343 penetrates one end of the bearing plate 342 away from the fixed plate 341. The cross section of the through groove 343 is substantially U-shaped. The extension rods 322 at both ends of the sorting member 32 can extend from the two through slots 343 respectively, and the main body 321 of the sorting member 32 is clamped between the two bearing plates 342. The fixing seat 34 improves the connection stability of the sorting member 32 on the mounting cover 31.

[0105] like Figure 1 and Figure 2As shown, in some embodiments, a sensor 14 is further provided on the first mounting seat 11, and a sensor sheet 15 is provided on the second mounting seat 21. The sensor 14 can detect the position of the sensor sheet 15 by electromagnetic induction or the like. The sensor 14 can be fixed to the edge of the first mounting seat 11 (the side of the slide rail 133 away from the rack 132). Along the extension direction of the slide rail 133, a plurality of sensors 14 can be provided to perform positive limit detection, negative limit detection, origin detection and in-place detection on the magnetic mechanism 20. In this embodiment, four sensors 14 are provided. When the leftmost sensor 14 detects the sensor sheet 15, the leftmost sensor 14 is triggered and an alarm will be sounded, indicating that the second mounting seat 21 can no longer move to the left, and the drive mechanism 10 will immediately respond to stop driving, which is a positive limit detection. When the rightmost sensor 14 detects the sensing sheet 15, the rightmost sensor 14 is triggered and an alarm is sounded, indicating that the second mounting seat 21 can no longer move to the right, and the drive mechanism 10 will immediately respond and stop driving. This is a negative limit detection. When the second sensor 14 from left to right detects the sensing sheet 15, it indicates that the magnetic separation device 100 is in the second state, the magnetic part 23 is outside the cavity 310 of the mounting cover 31, and the separation part 32 is outside the magnetic field range of the magnetic part 23. This is an origin detection. When the third sensor 14 from left to right detects the sensing sheet 15, it indicates that the magnetic separation device 100 is in the first state, the magnetic part 23 is in the cavity 310, and the separation part 32 is within the magnetic field range of the magnetic part 23. This is an in-place detection.

[0106] When in use, the drive unit 12 is started to rotate (forward), the drive unit 12 drives the gear 131 to rotate (forward), the gear 131 rotates and drives the rack 132 to move, thereby driving the second mounting seat 21 on the rack 132 and the magnetic mechanism 20 to move toward the sorting mechanism 30 (sorting member 32). Initially, the second sensor 14 from the left to the right will detect the induction sheet 15 (in-situ detection), the magnetic member 23 is outside the cavity 310 of the mounting cover 31, and the sorting member 32 is outside the magnetic field range of the magnetic member 23. The second mounting seat 21 and the magnetic mechanism 20 continue to move toward the sorting mechanism 30. When the third sensor 14 from the left to the right detects the induction sheet 15 (arrival detection), it indicates that the magnetic member 23 has moved into the cavity 310 of the mounting cover 31, and the sorting member 32 is located at the position where the magnetic field of the magnetic member 23 is the strongest (for example, when in this position, the center line of the two magnetic members 23 arranged opposite to each other at the same height passes through the center of the circular surface of the sorting member 32 at this height, such as Figure 3As shown), the magnetic particles 33 in the separation component 32 have strong magnetism. At this time, the driving unit is stopped to keep the magnetic component 23 in this position, and the sample solution is flowed through the separation component 32. The target object with magnetic particles in the solution will be adsorbed on the surface of the separation component 32 due to the suction force of the magnetic particles 33 in the separation component 32, and other impurities will flow away. When the sample solution has flowed out, the driving unit 12 is started to rotate (reverse), and the driving unit 12 drives the gear 131 to rotate (reverse). The rotation of the gear 131 drives the rack 132 to move, thereby driving the magnetic mechanism 20 on the rack 132 to move in a direction away from the separation mechanism 30 (separation component 32), so that the magnetic component 23 is moved out of the cavity 310 of the mounting cover 31, as shown in FIG. Figure 4 As shown. At this time, the magnetic field at the location of the sorting element 32 is reduced to the weakest, and the magnetic particles 33 in the sorting element 32 are weakly magnetic or even non-magnetic, and cannot attract the target cells with magnetic particles. When the eluent flows through the sorting element 32, the target objects with magnetic particles will be washed away by the eluent, and the collected eluent will only contain the desired target objects.

[0107] In the magnetic separation device 100 of the present application, the magnetic part 23 moves under the action of the driving unit 12 and the transmission unit 13, so that the separation part 32 is located within the magnetic field range of the magnetic part 23 or outside the magnetic field range of the magnetic part 23, so that the magnetic field can be loaded or eliminated on the separation part 32, and the automation of loading and removing the magnetic field is realized. Since the mechanical structure of the magnetic separation device 100 is determined, each magnetic field loading and elimination has a strong consistency, so the separation process of the target object can maintain good consistency.

[0108] The above descriptions are some specific implementations of the present application, but they are not limited to these implementations in actual application. For ordinary technicians in this field, other variations and changes made according to the technical concept of the present application should all fall within the protection scope of the present application.

Claims

1. A magnetic separation device for separating a target object from a sample, characterized in that: The magnetic separation device comprises: A driving mechanism, the driving mechanism comprising a first mounting seat and a driving unit and a transmission unit arranged on the first mounting seat, the driving unit being used to generate power and drive the transmission unit to move, and the transmission unit being used to transmit the power; A magnetic mechanism, the magnetic mechanism comprising a magnetic member, the magnetic member being disposed on the transmission unit and being movable under the drive of the transmission unit; and A sorting mechanism, the sorting mechanism comprising a mounting cover and a sorting member arranged on the mounting cover, the mounting cover comprising a cavity, the opening of the cavity facing the magnetic mechanism, the top wall of the cavity facing away from the magnetic mechanism is recessed toward the opening of the cavity to form a receiving groove, and the sorting member is arranged in the receiving groove; Wherein, the magnetic separation device has a first state and a second state. When the magnetic separation device is in the first state, the magnetic component is located in the cavity, and the separation component is located within the magnetic field range of the magnetic component; when the magnetic separation device is in the second state, the magnetic component is outside the cavity, and the separation component is located outside the magnetic field range of the magnetic component.

2. The magnetic separation device according to claim 1, characterized in that: The magnetic mechanism further includes a second mounting seat, the magnetic component is arranged on the second mounting seat, and the second mounting seat is fixed to the transmission unit.

3. The magnetic separation device according to claim 2, characterized in that: The magnetic mechanism also includes a magnetic seat fixed on the second mounting seat, and the magnetic seat includes a bottom plate and two oppositely arranged side plates extending from the edge of the bottom plate in the same direction, the edge of each side plate extends toward the other side plate to form an extension plate, the extension plate, the side plate and the bottom plate together form a groove, and the surface of each side plate facing the other side plate is recessed in a direction away from the other side plate to form a receiving groove, and the magnetic member is arranged in the receiving groove.

4. The magnetic separation device according to claim 3, characterized in that: The edge of the extension plate extends toward the direction close to the bottom plate to form a protrusion.

5. The magnetic separation device according to claim 3, characterized in that: An iron plate is also provided in the receiving groove, and the iron plate is located between the magnetic member and the side plate.

6. The magnetic separation device as claimed in claim 3, wherein a plurality of magnetic parts are arranged in the receiving groove.

7. The magnetic separation device according to claim 1, characterized in that: The sorting component comprises a main body and extension rods arranged at two ends of the main body, filter plates are respectively arranged at two ends of the main body, a plurality of magnetic particles are arranged in the main body, and the magnetic particles are located between the filter plates.

8. The magnetic separation device according to claim 7, characterized in that: The sorting mechanism also includes a fixed seat, which is arranged in the accommodating groove. The fixed seat includes a fixed plate and a supporting plate arranged at both ends of the fixed plate. The supporting plate is provided with a through groove that penetrates the supporting plate along the thickness direction. The extension rod extends from the through groove, and the main body is clamped between the supporting plates.

9. The magnetic separation device according to claim 1, characterized in that: The driving unit includes a rotating shaft, and the transmission unit includes a gear and a rack meshing with the gear. The gear is sleeved on the rotating shaft, and the rack is disposed on the first mounting seat and is configured to be movable on the first mounting seat.

10. The magnetic separation device according to claim 9, characterized in that: The transmission unit further includes a slide rail and a slider disposed on the first mounting seat, the slider being configured to slide on the slide rail, and the magnetic mechanism further includes a second mounting seat, the second mounting seat being fixed on the rack and the slider.

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