Cellulose-containing soil nitrogen synergist and preparation method thereof

Abstract

The invention relates to the technical field of nitrogen synergists, and discloses a cellulose-containing soil nitrogen synergist and a preparation method thereof, the cellulose-containing soil nitrogen synergist is prepared from the following components: a urease inhibitor, sodium dodecyl benzene sulfonate, composite cellulose, zeolite powder and EM bacteria; the soil nitrogen synergist prepared by the invention can greatly reduce the use amount of nitrogen, so that the problem of soil hardening caused by excessive use of chemical fertilizers can be effectively prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of nitrogen synergists, in particular to a cellulose-containing soil nitrogen synergist and a preparation method thereof. Background Art

[0002] Nitrogen is one of the essential macronutrients for the growth and development of crops. However, the original nitrogen content in the soil is relatively low and cannot meet the growth needs of crops. Therefore, additional nitrogen fertilizer is needed to increase the soil's nitrogen supply capacity and thus maintain high crop yields.

[0003] However, in order to greatly increase the nitrogen content in the soil, chemical nitrogen fertilizers are usually used in large quantities. However, long-term use of chemical nitrogen fertilizers will have adverse effects on the physical and chemical properties of the soil, the quality and safety of agricultural products, and the ecological environment.

[0004] Soil nitrogen synergists are a class of chemical substances that can improve the utilization rate of nitrogen in the soil. However, the effects of soil nitrogen synergists prepared by existing technologies are relatively general. Therefore, in order to reduce the use of chemical nitrogen fertilizers, existing soil nitrogen synergists have been improved to obtain nitrogen synergists with higher synergy.

[0005] Based on this, we proposed a cellulose-containing soil nitrogen enhancer. Summary of the Invention

[0006] (1) Technical problems solved

[0007] In view of the deficiencies of the prior art, the present invention provides a cellulose-containing soil nitrogen enhancer and a preparation method thereof.

[0008] (2) Technical solution

[0009] To achieve the above-mentioned purpose, the present invention provides the following technical solutions:

[0010] A cellulose-containing soil nitrogen synergist comprises the following ingredients in parts by weight: 0.1-0.3 parts of a urease inhibitor, 2-5 parts of sodium dodecylbenzenesulfonate, 30-40 parts of composite cellulose, 5-8 parts of zeolite powder and 0.2-0.4 parts of EM bacteria.

[0011] As a further technical solution, the method for preparing composite cellulose comprises the following steps:

[0012] (1) Weigh alfalfa, willow powder, and eucalyptus leaves separately;

[0013] (2) Cleaning: Wash alfalfa, willow powder, and eucalyptus leaves for 10 min each, filter, and drain;

[0014] (3) Pretreatment: pretreating the willow wood powder to obtain pretreated willow wood powder;

[0015] (4) mixing: mixing alfalfa, pretreated willow wood powder and eucalyptus leaves to obtain a mixture;

[0016] (5) preparing sodium hydroxide solution;

[0017] The mass fraction of sodium hydroxide solution is 5%;

[0018] (6) Alkali treatment: Mix the mixture with sodium hydroxide solution, heat it in a water bath to 85°C, keep it warm and stir for 3 hours, then use acetic acid solution to adjust the pH of the system to 4.5, adjust the temperature to 78°C, continue stirring for 30 minutes, then filter and wash it until it is neutral to obtain an intermediate material;

[0019] (7) Bleaching treatment:

[0020] The obtained intermediate material is mixed with bleaching liquid, bleached for 10 minutes, and then filtered and washed until neutral to obtain a bleached material;

[0021] (8) Hydrothermal treatment:

[0022] The obtained bleached material was mixed with water in a mass ratio of 1:5, heated to 100° C., stirred for 4 hours, then filtered and freeze-dried to obtain composite cellulose.

[0023] As a further technical solution: the mass ratio of alfalfa, willow powder and eucalyptus leaves in step (1) is 10:3:1.

[0024] As a further technical solution: the method for preparing the pretreated willow wood powder in step (3) is:

[0025] First, willow wood powder and water are mixed to obtain a willow wood powder mixed liquid;

[0026] The willow wood powder mixture is heated to 80° C., kept warm for 15 minutes, and then pulped for 30 minutes to obtain a willow wood powder slurry;

[0027] The temperature of the willow wood powder slurry was adjusted to 38° C. and kept warm for 30 minutes. Then, the treatment enzyme was added to the willow wood powder slurry, stirred for 2 hours, and then subjected to rotary drying and pulverization to obtain pretreated willow wood powder.

[0028] As a further technical solution: the mass ratio of alfalfa, willow powder and eucalyptus leaves in step (1) is 10:3:1.

[0029] As a further technical solution: the mass ratio of willow wood powder to water is 1:10;

[0030] The beating speed is 6000r / min;

[0031] The mass ratio of the willow wood powder slurry and the treated enzyme was 100:3;

[0032] The treatment enzyme is a mixture of β-glucanase and pectinase;

[0033] The mixing mass ratio of β-glucanase and pectinase is 1:3.

[0034] As a further technical solution: in step (6), the mixing ratio of the mixture and the sodium hydroxide solution is 2-3 g: 100 mL;

[0035] The mass fraction of the acetic acid solution is 8%.

[0036] As a further technical solution: the mixing ratio of the intermediate material and the bleaching liquid in step (7) is 10g:90mL;

[0037] The bleaching liquid is a sodium hypochlorite solution with a mass fraction of 10%.

[0038] A method for preparing a cellulose-containing soil nitrogen synergist comprises:

[0039] Weigh the following according to their weight parts: urease inhibitor, sodium dodecylbenzenesulfonate, composite cellulose, zeolite powder and EM bacteria;

[0040] The urease inhibitor, sodium dodecylbenzenesulfonate, composite cellulose, zeolite powder and EM bacteria are sequentially added into a mixer, stirred and mixed, dried until the moisture content is less than 5%, and packaged.

[0041] As a further technical solution, the drying temperature is 50° C. and the drying time is 10 hours.

[0042] (3) Beneficial effects

[0043] Compared with the prior art, the present invention provides a cellulose-containing soil nitrogen enhancer with the following beneficial effects:

[0044] The soil nitrogen synergist prepared by the present invention can greatly reduce the amount of nitrogen used, thereby effectively preventing the problem of soil compaction caused by excessive use of chemical fertilizers.

[0045] The urease inhibitor in the soil nitrogen synergist prepared by the present invention can significantly reduce the hydrolysis rate of urea in the soil by inhibiting the activity of urease in the soil, thereby greatly reducing the volatilization loss of ammonia caused by urea hydrolysis, reducing nitrogen loss, and enabling more nitrogen in the soil to be absorbed and utilized by crops. Since the nitrogen loss is reduced, the use of chemical fertilizers can be significantly reduced.

[0046] The present invention introduces composite cellulose, which has rich functional groups such as hydroxyl groups. It can adsorb ammonium nitrogen in the soil through forces such as hydrogen bonds, and fix it on the surface and internal pores of the composite cellulose, thereby reducing the risk of ammonium nitrogen loss. The adsorbed nitrogen will be gradually released as the composite cellulose decomposes in the soil, continuously providing nitrogen nutrition to plants, extending the effective supply period of nitrogen, and improving nitrogen utilization.

[0047] As a nitrogen enhancer, composite cellulose may also enhance the plant's assimilation of nitrogen by regulating hormone levels and metabolic processes in crops, enabling crops to more effectively convert absorbed nitrogen into their own organic matter and improve nitrogen utilization efficiency.

[0048] Denitrification is the process by which nitrate nitrogen in the soil is reduced to gases such as nitrogen and lost under anaerobic conditions. Cellulose, combined with other ingredients, can improve soil aeration and moisture levels, reducing the formation of anaerobic areas in the soil and thus reducing the occurrence of denitrification. DETAILED DESCRIPTION

[0049] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0050] Example 1

[0051] A method for preparing a cellulose-containing soil nitrogen synergist comprises:

[0052] Weigh the following in parts by weight: 0.1 part of urease inhibitor, 2 parts of sodium dodecylbenzenesulfonate, 30 parts of composite cellulose, 5 parts of zeolite powder and 0.2 parts of EM bacteria; the composite cellulose preparation method comprises the following steps:

[0053] (1) Weigh alfalfa, willow powder, and eucalyptus leaves separately;

[0054] The mass ratio of alfalfa, willow powder and eucalyptus leaves is 10:3:1;

[0055] (2) Cleaning: Wash alfalfa, willow powder, and eucalyptus leaves for 10 min each, filter, and drain;

[0056] (3) Pretreatment: Pretreating the willow wood powder to obtain pretreated willow wood powder; the preparation method of the pretreated willow wood powder is as follows:

[0057] First, willow wood powder and water are mixed to obtain a willow wood powder mixed liquid;

[0058] The willow wood powder mixture is heated to 80° C., kept warm for 15 minutes, and then pulped for 30 minutes to obtain a willow wood powder slurry;

[0059] The temperature of the willow wood powder slurry was adjusted to 38° C. and kept warm for 30 minutes. Then, the treatment enzyme was added to the willow wood powder slurry, stirred for 2 hours, and then subjected to rotary drying and pulverization to obtain pretreated willow wood powder.

[0060] The mass ratio of willow wood powder to water is 1:10;

[0061] The beating speed is 6000r / min;

[0062] The mass ratio of the willow wood powder slurry and the treated enzyme was 100:3;

[0063] The treatment enzyme is a mixture of β-glucanase and pectinase;

[0064] The mixing mass ratio of β-glucanase and pectinase is 1:3.

[0065] (4) mixing: mixing alfalfa, pretreated willow wood powder and eucalyptus leaves to obtain a mixture;

[0066] (5) preparing sodium hydroxide solution;

[0067] The mass fraction of sodium hydroxide solution is 5%;

[0068] (6) Alkali treatment: The mixture and sodium hydroxide solution were mixed, heated in a water bath to 85°C, and stirred for 3 hours. Then, the pH of the system was adjusted to 4.5 with acetic acid solution, and the temperature was adjusted to 78°C. The mixture was stirred for 30 minutes, and then filtered and washed until neutral to obtain an intermediate material. The mixing ratio of the mixture and sodium hydroxide solution was 2g:100mL.

[0069] The mass fraction of the acetic acid solution is 8%.

[0070] (7) Bleaching treatment:

[0071] The obtained intermediate material is mixed with bleaching liquid, bleached for 10 minutes, and then filtered and washed until neutral to obtain a bleached material; the mixing ratio of the intermediate material and the bleaching liquid is 10g:90mL;

[0072] The bleaching liquid is a sodium hypochlorite solution with a mass fraction of 10%.

[0073] (8) Hydrothermal treatment:

[0074] The obtained bleached material was mixed with water in a mass ratio of 1:5, heated to 100° C., stirred for 4 hours, then filtered and freeze-dried to obtain composite cellulose.

[0075] The urease inhibitor, sodium dodecylbenzenesulfonate, composite cellulose, zeolite powder and EM bacteria are sequentially added to a mixer, stirred and mixed, dried until the moisture content is less than 5%, and packaged; the drying temperature is 50° C. and the time is 10 hours.

[0076] Example 2

[0077] A method for preparing a cellulose-containing soil nitrogen synergist comprises:

[0078] Weigh the following in parts by weight: 0.2 parts of urease inhibitor, 3 parts of sodium dodecylbenzenesulfonate, 34 parts of composite cellulose, 6 parts of zeolite powder and 0.25 parts of EM bacteria; the composite cellulose preparation method comprises the following steps:

[0079] (1) Weigh alfalfa, willow powder, and eucalyptus leaves separately;

[0080] The mass ratio of alfalfa, willow powder and eucalyptus leaves is 10:3:1;

[0081] (2) Cleaning: Wash alfalfa, willow powder, and eucalyptus leaves for 10 min each, filter, and drain;

[0082] (3) Pretreatment: Pretreating the willow wood powder to obtain pretreated willow wood powder; the preparation method of the pretreated willow wood powder is as follows:

[0083] First, willow wood powder and water are mixed to obtain a willow wood powder mixed liquid;

[0084] The willow wood powder mixture is heated to 80° C., kept warm for 15 minutes, and then pulped for 30 minutes to obtain a willow wood powder slurry;

[0085] The temperature of the willow wood powder slurry was adjusted to 38° C. and kept warm for 30 minutes. Then, the treatment enzyme was added to the willow wood powder slurry, stirred for 2 hours, and then subjected to rotary drying and pulverization to obtain pretreated willow wood powder.

[0086] The mass ratio of willow wood powder to water is 1:10;

[0087] The beating speed is 6000r / min;

[0088] The mass ratio of the willow wood powder slurry and the treated enzyme was 100:3;

[0089] The treatment enzyme is a mixture of β-glucanase and pectinase;

[0090] The mixing mass ratio of β-glucanase and pectinase is 1:3.

[0091] (4) mixing: mixing alfalfa, pretreated willow wood powder and eucalyptus leaves to obtain a mixture;

[0092] (5) preparing sodium hydroxide solution;

[0093] The mass fraction of sodium hydroxide solution is 5%;

[0094] (6) Alkali treatment: Mix the mixture and sodium hydroxide solution, heat in a water bath to 85°C, keep stirring for 3 hours, then adjust the pH of the system to 4.5 with acetic acid solution, adjust the temperature to 78°C, continue stirring for 30 minutes, then filter and wash until neutral to obtain an intermediate material; the mixing ratio of the mixture and sodium hydroxide solution is 2-3g:100mL;

[0095] The mass fraction of the acetic acid solution is 8%.

[0096] (7) Bleaching treatment:

[0097] The obtained intermediate material is mixed with bleaching liquid, bleached for 10 minutes, and then filtered and washed until neutral to obtain a bleached material; the mixing ratio of the intermediate material and the bleaching liquid is 10g:90mL;

[0098] The bleaching liquid is a sodium hypochlorite solution with a mass fraction of 10%.

[0099] (8) Hydrothermal treatment:

[0100] The obtained bleached material was mixed with water in a mass ratio of 1:5, heated to 100° C., stirred for 4 hours, then filtered and freeze-dried to obtain composite cellulose.

[0101] The urease inhibitor, sodium dodecylbenzenesulfonate, composite cellulose, zeolite powder and EM bacteria are sequentially added to a mixer, stirred and mixed, dried until the moisture content is less than 5%, and packaged; the drying temperature is 50° C. and the time is 10 hours.

[0102] Example 3

[0103] A method for preparing a cellulose-containing soil nitrogen synergist comprises:

[0104] Weigh the following in parts by weight: 0.3 parts of urease inhibitor, 5 parts of sodium dodecylbenzenesulfonate, 40 parts of composite cellulose, 8 parts of zeolite powder and 0.4 parts of EM bacteria; the composite cellulose preparation method comprises the following steps:

[0105] (1) Weigh alfalfa, willow powder, and eucalyptus leaves separately;

[0106] The mass ratio of alfalfa, willow powder and eucalyptus leaves is 10:3:1;

[0107] (2) Cleaning: Wash alfalfa, willow powder, and eucalyptus leaves for 10 min each, filter, and drain;

[0108] (3) Pretreatment: Pretreating the willow wood powder to obtain pretreated willow wood powder; the preparation method of the pretreated willow wood powder is as follows:

[0109] First, willow wood powder and water are mixed to obtain a willow wood powder mixed liquid;

[0110] The willow wood powder mixture is heated to 80° C., kept warm for 15 minutes, and then pulped for 30 minutes to obtain a willow wood powder slurry;

[0111] The temperature of the willow wood powder slurry was adjusted to 38° C. and kept warm for 30 minutes. Then, the treatment enzyme was added to the willow wood powder slurry, stirred for 2 hours, and then subjected to rotary drying and pulverization to obtain pretreated willow wood powder.

[0112] The mass ratio of willow wood powder to water is 1:10;

[0113] The beating speed is 6000r / min;

[0114] The mass ratio of the willow wood powder slurry and the treated enzyme was 100:3;

[0115] The treatment enzyme is a mixture of β-glucanase and pectinase;

[0116] The mixing mass ratio of β-glucanase and pectinase is 1:3.

[0117] (4) mixing: mixing alfalfa, pretreated willow wood powder and eucalyptus leaves to obtain a mixture;

[0118] (5) preparing sodium hydroxide solution;

[0119] The mass fraction of sodium hydroxide solution is 5%;

[0120] (6) Alkali treatment: Mix the mixture and sodium hydroxide solution, heat in a water bath to 85°C, keep stirring for 3 hours, then adjust the pH of the system to 4.5 with acetic acid solution, adjust the temperature to 78°C, continue stirring for 30 minutes, then filter and wash until neutral to obtain an intermediate material; the mixing ratio of the mixture and sodium hydroxide solution is 2-3g:100mL;

[0121] The mass fraction of the acetic acid solution is 8%.

[0122] (7) Bleaching treatment:

[0123] The obtained intermediate material is mixed with bleaching liquid, bleached for 10 minutes, and then filtered and washed until neutral to obtain a bleached material; the mixing ratio of the intermediate material and the bleaching liquid is 10g:90mL;

[0124] The bleaching liquid is a sodium hypochlorite solution with a mass fraction of 10%.

[0125] (8) Hydrothermal treatment:

[0126] The obtained bleached material was mixed with water in a mass ratio of 1:5, heated to 100° C., stirred for 4 hours, then filtered and freeze-dried to obtain composite cellulose.

[0127] The urease inhibitor, sodium dodecylbenzenesulfonate, composite cellulose, zeolite powder and EM bacteria are sequentially added to a mixer, stirred and mixed, dried until the moisture content is less than 5%, and packaged; the drying temperature is 50° C. and the time is 10 hours.

[0128] The following are comparative examples:

[0129] Comparative Example 1:

[0130] On the basis of Example 1, no composite cellulose was added, and the rest of the technical solutions remained consistent with those of Example 1.

[0131] Comparative Example 2:

[0132] On the basis of Example 1, alfalfa was not added during the preparation of composite cellulose, and the rest of the technical solutions remained consistent with those of Example 1.

[0133] Comparative Example 3:

[0134] On the basis of Example 1, alfalfa and eucalyptus leaves were not added during the preparation of composite cellulose, and the rest of the technical solutions remained consistent with those of Example 1.

[0135] Comparative Example 4:

[0136] On the basis of Example 1, eucalyptus leaves were not added during the preparation of composite cellulose, and the rest of the technical solutions remained consistent with those of Example 1.

[0137] test:

[0138] Vegetable greenhouses were used as the experimental base, each with a 4×5m 2 Size, set up 8 groups in total, set up a blank control group, no nitrogen synergist was used, fertilization was exactly the same, the total nitrogen content of the blank control group fertilizer was 20kg ± 0.1kg per mu, among which 7 groups were added with nitrogen synergist, and the nitrogen content in the fertilizer was reduced by 20% by mass, and the tomato yield (big red tomato 7845) grown by them was compared;

[0139] Table 1

[0140] Tomato yield kg / mu Example 1 7315 Example 2 7228 Example 3 7267 Comparative Example 1 4669 Comparative Example 2 6563 Comparative Example 3 6032 Comparative Example 4 6681 Blank control group 5103

[0141] It can be seen from Table 1 that the nitrogen synergist prepared by the present invention can significantly reduce the amount of nitrogen used and, at the same time, can also increase the yield of crops.

[0142] Using Example 1 as the base sample, the total nitrogen content in the fertilizer was 20 kg ± 0.1 kg per mu. As a basis, when the nitrogen synergist of Example 1 was added, the yield of large red tomatoes was compared under the conditions where the nitrogen content in different fertilizers was reduced in proportion to their mass.

[0143] Table 2

[0144]

[0145]

[0146] It can be seen from Table 2 that as the nitrogen usage decreases, the tomato yield is high within a certain ratio. However, after exceeding a certain reduction ratio, the synergistic effect of the nitrogen synergist cannot meet the needs of tomatoes, which will gradually lead to a decrease in tomato yield.

[0147] The vitamin C content in the tomatoes of the embodiment was tested and compared:

[0148] Table 3

[0149] Vitamin C mg / 100g Example 1 19.32 Example 2 19.04 Example 3 19.25 Comparative Example 1 17.63 Comparative Example 2 18.65 Comparative Example 3 18.01 Comparative Example 4 18.46 Blank control group 17.04

[0150] It can be seen from Table 3 that the nitrogen synergist of the present invention can significantly improve the quality of tomatoes and effectively increase the content of vitamin C in tomatoes.

[0151] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A cellulose-containing soil nitrogen enhancer, characterized in that: The invention comprises the following ingredients by weight: 0.1-0.3 parts of urease inhibitor, 2-5 parts of sodium dodecylbenzene sulfonate, 30-40 parts of composite cellulose, 5-8 parts of zeolite powder and 0.2-0.4 parts of EM bacteria.

2. The cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: The composite cellulose preparation method comprises the following steps: (1) Weigh alfalfa, willow wood powder and eucalyptus leaves respectively; (2) Cleaning: Clean the alfalfa, willow powder and eucalyptus leaves for 10 min, filter and drain; (3) Pretreatment: pretreating the willow wood powder to obtain pretreated willow wood powder; (4) mixing: mixing alfalfa, pretreated willow wood powder and eucalyptus leaves to obtain a mixture; (5) preparing sodium hydroxide solution; The mass fraction of sodium hydroxide solution is 5%; (6) Alkali treatment: the mixed material and sodium hydroxide solution are mixed, heated to 85°C in a water bath, and stirred for 3 hours at this temperature. Then, the pH of the system is adjusted to 4.5 with acetic acid solution, and the temperature is adjusted to 78°C. The mixture is stirred for 30 minutes, filtered, and washed to neutrality to obtain an intermediate material. (7) Bleaching treatment: The obtained intermediate material is mixed with the bleaching liquid, bleached for 10 minutes, and then filtered and washed to neutrality to obtain a bleached material; (8) Hydrothermal treatment: The obtained bleached material was mixed with water in a mass ratio of 1:5, heated to 100° C., stirred for 4 hours, filtered, and freeze-dried to obtain composite cellulose.

3. The cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: The mass ratio of alfalfa, willow powder and eucalyptus leaves in step (1) is 10:3:

1.

4. The cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: The method for preparing the pretreated willow wood powder in step (3) is as follows: First, willow wood powder and water are mixed to obtain a willow wood powder mixed liquid; The willow wood powder mixed liquid is heated to 80° C., kept warm for 15 minutes, and then pulped for 30 minutes to obtain a willow wood powder slurry; The temperature of the willow powder slurry was adjusted to 38° C. and kept warm for 30 minutes. Then, the treatment enzyme was added to the willow powder slurry, stirred for 2 hours, and then rotary dried and crushed to obtain pretreated willow powder.

5. The cellulose-containing soil nitrogen enhancer according to claim 4, characterized in that: The mass ratio of willow wood powder to water is 1:10; The beating speed is 6000r / min; The mass ratio of willow wood powder slurry and treated enzyme was 100:3; The treatment enzyme is a mixture of β-glucanase and pectinase; The mixing mass ratio of β-glucanase to pectinase is 1:

3.

6. The cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: In step (6), the mixing ratio of the mixed material and the sodium hydroxide solution is 2-3 g: 100 mL; The mass fraction of the acetic acid solution is 8%.

7. The cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: The mixing ratio of the intermediate material and the bleaching liquid in step (7) is 10 g:90 mL; The bleaching liquid is a sodium hypochlorite solution with a mass fraction of 10%.

8. The method for preparing a cellulose-containing soil nitrogen enhancer according to claim 1, characterized in that: include: Weigh the following according to weight: urease inhibitor, sodium dodecylbenzene sulfonate, composite cellulose, zeolite powder and EM bacteria; The urease inhibitor, sodium dodecylbenzene sulfonate, composite cellulose, zeolite powder and EM bacteria are sequentially added into a mixer, stirred and mixed, dried until the water content is less than 5%, and packaged.

9. The cellulose-containing soil nitrogen enhancer according to claim 8, characterized in that: The drying temperature was 50°C and the drying time was 10 hours.

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