Micro-fluidic chip suitable for coal microbial gasification

Abstract

The invention relates to the technical field of coal fluidized mining, in particular to a micro-fluidic chip suitable for coal microbial gasification, which comprises a raw material mixing system, a degradation reaction system, a gas-liquid detection system and a biological separation system. The raw material mixing system is connected with the degradation reaction system through a pipeline, the degradation reaction system is connected with the gas and liquid detection system, and the gas detection system is connected with the biological separation system. Operation, result monitoring, data regulation and control and the like of the whole device are controlled by the intelligent control system. The device is high in controllability, high in convenience and convenient to operate, the coal microbial gasification process can be observed from the microscopic perspective, the essence of the coal microbial gasification reaction is explored, and the device has a wide application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of fluidized coal mining, in particular to a microfluidic chip suitable for coal microbial gasification. Background Art

[0002] Coal microbial gasification technology is based on the theory of anaerobic fermentation. It aims to degrade organic components in coal through the special metabolic pathways and bio-enzyme systems of native microorganisms in coal seams to produce methane, CO2, light hydrocarbons and other products. Coal biodegradation technology has the characteristics of cleanliness, high efficiency and flexibility, and has considerable potential in improving the efficiency of coal mining and utilization. At present, the efficiency of methane production by this method is low. The reason is that the structure of coal is complex, and the organic matter contained, such as lignin, cycloalkanes, aromatic compounds, etc., are difficult to hydrolyze, and it is not an ideal matrix for biodegradation. Methanogens can only use limited compounds such as CO2, acetic acid, and methyl compounds to produce methane; the community diversity is high during anaerobic degradation, the metabolic mechanism of the microbial community is complex, and some microbial communities have competitive relationships; the energy cost of microbial life activities determines the tolerance concentration of inorganic salts, and different methanogens have different tolerance limits. There are differences in the effectiveness of nutrients, and the anaerobic degradation process of different pathways is limited. At present, the means of increasing methane production are mostly to add metabolic intermediates, nutrients, trace elements, etc. to improve microbial activity. Therefore, from a microscopic perspective, it is of great significance to clarify the degradation pathways of key intermediates, understand the response characteristics of microbial communities to external stimuli, and explain the production pathways of biomethane.

[0003] Microfluidic chip is a kind of science and technology that mainly operates fluids in micrometer-scale space. It concentrates the basic operating units in fields such as chemistry and biology on a chip of a few square centimeters. The microchannels form a network and controllable fluids run through the entire system. It has the advantages of high analysis efficiency, accurate quantitative analysis, high reproducibility, and low reagent consumption. Chip generators based on microfluidic technology have been widely used in the fields of drug activity screening, cell and microbial culture, tissue and organ bionics, trace substance detection, and gas pollutant risk assessment.

[0004] In microfluidic chips, the strategy for gas production is usually to expose a single concentration of gas in a single experiment. This chip device with low test throughput is not suitable for the complex gas environment of coal microbial gasification. In addition, multi-unit operations at different steps of anaerobic degradation often have different temperature, anaerobic and other requirements. The simple superposition of existing microfluidic chips cannot achieve precise temperature control and anaerobic conditions for each area. Therefore, it is urgently needed to develop a microfluidic chip suitable for coal microbial gasification that can be used in laboratories. The chip integrates reaction, collection, and detection, and integrates different variable gradient generation units into the chip device. The dependency between variable gradients and reaction pathways can be clarified in a one-time experiment, thereby enhancing the bionic ability of coal microbial degradation under in situ conditions. Summary of the invention

[0005] The purpose of the present invention is to solve the problems existing in the prior art, and to propose a microfluidic chip suitable for coal microbial gasification, which is used to explore the morphological changes and degradation pathways of coal during coal microbial gasification under microscopic conditions. It integrates reaction, collection and detection, and can meet the needs of multiple experiments.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A microfluidic chip suitable for coal microbial gasification, comprising a raw material mixing system, a degradation reaction system, a gas and liquid detection system, and a biological separation system arranged on a chip substrate, wherein the raw material mixing system is connected to the degradation reaction system through a pipeline, the degradation reaction system is connected to the gas and liquid detection system, and the gas detection system is connected to the biological separation system;

[0008] The raw material mixing system comprises a liquid mixing chamber, on which a liquid inlet branch pipe and a bacteria inlet branch pipe are arranged, and the liquid inlet branch pipe and the bacteria inlet branch pipe are connected to the liquid mixing chamber through a liquid inlet main channel respectively;

[0009] The degradation reaction system includes a mixed liquid main channel, a sample outlet main channel and a plurality of anaerobic degradation reaction chambers. The mixed liquid main channel is connected to the liquid mixing chamber. The plurality of anaerobic degradation reaction chambers are connected to the mixed liquid main channel through sample inlet branch channels, respectively. The plurality of anaerobic degradation reaction chambers are connected to the sample outlet main channel through sample outlet branch channels, respectively. The sample inlet branch channels are also provided with liquid inlet channels for feeding different ion solutions into the anaerobic degradation reaction chambers. The anaerobic degradation reaction chambers can be selectively used, and other anaerobic degradation reaction chambers are closed.

[0010] The gas and liquid detection system includes a storage device, a gas detection device, a liquid detection device, and an anaerobic device. The storage device is connected to the main sample outlet channel. The storage device is connected to the gas detection device through an air inlet channel, connected to the liquid detection device through a liquid inlet channel, and connected to the anaerobic device through an air inlet channel.

[0011] The biological separation system includes an electrode cavity, an excitation circuit module, a biological storage area, and a waste liquid area. A microelectrode positive electrode and a microelectrode negative electrode are arranged on the electrode cavity along the liquid flow direction. The excitation circuit module is connected to the microelectrode positive electrode and the microelectrode negative electrode through wires to form a loop; the electrode cavity is connected to the storage device through a discharge channel, and is connected to the biological storage area through a first sorting sample channel. The first sorting sample channel is close to the microelectrode positive electrode and the microelectrode negative electrode side, and is connected to the waste liquid area through a second sorting sample channel. The microelectrode positive electrode and the microelectrode negative electrode generate a non-uniform electric field, adsorb the coal powder in the solution in the electrode cavity, gather the coal powder to the microelectrode positive electrode and the microelectrode negative electrode side, and squeeze the bacteria in the solution to the opposite side, so that the bacteria enter the first sorting sample channel, and the coal powder enters the second sorting sample channel.

[0012] As a further preferred solution, an NTC thermoelectric probe is provided on the anaerobic degradation reaction chamber. The thermoelectric probe adopts a thermocouple and is connected to an external temperature collector.

[0013] As a further preferred solution, multiple through holes are opened around the multiple anaerobic degradation reaction chambers, multiple circuit layer 1s are printed on the front side of the chip substrate, and multiple circuit layer 2s are printed on the back side. Circuit layer 1 and circuit layer 2 are electrically connected through copper plating at the through holes. Circuit layer 1 and circuit layer 2 are alternately connected to realize a series circuit, and the series circuit is connected to an external temperature controller.

[0014] As a further preferred embodiment, the storage device includes a lower layer liquid storage device for storing solution and an upper layer gas storage device for storing gas, the discharge channel and the liquid inlet channel are connected to the liquid storage device, and the gas inlet channel is connected to the gas storage device.

[0015] As a further preferred solution, a partition is provided between the gas storage device and the liquid storage device, and a main inlet for the inert gas is provided on the partition.

[0016] As a further preferred solution, the gas storage device is connected to the anaerobic device through an air inlet channel.

[0017] As a further preferred embodiment, the gas detection device includes a substrate, a microchannel is opened on the surface of the substrate, a polyvinyl alcohol coating is provided on the inner wall of the microchannel, one end of the microchannel is a gas inlet, and the other end is a microcavity for detection, a metal oxide semiconductor sensor based on redox reaction is installed in the microcavity, and the metal oxide semiconductor sensor consists of an integrated heater, interlaced platinum electrodes with metal oxide semiconductors, and a monitoring device.

[0018] As a further preferred scheme, a suspended matter filter and a valve one are provided on the liquid inlet branch pipe, a valve two is provided on the bacteria inlet branch pipe, a valve three is provided between the mixed liquid main channel and the liquid mixing chamber, a valve four is provided on the liquid inlet channel, a valve five is provided on the sample injection branch channel, a valve six is ​​provided on the sample outlet branch channel, and a valve seven is provided on the discharge channel.

[0019] By adopting the above technical solution, the present invention has the following beneficial effects:

[0020] 1. The microfluidic chip is equipped with multiple reaction channels, which can provide precise fluid manipulation and reaction condition control, thereby achieving precise management of the coal microbial gasification process.

[0021] 2. The chip design allows multiple reaction functions to be integrated on the same chip. This integrated design helps improve the convenience and reliability of the experiment.

[0022] 3. The reactants can be observed at the micron level, which helps to understand the reaction process of microbial degradation of coal.

[0023] 4. The chip can precisely adjust the flow rate of the fluid to ensure that the reactants in the gasification process are mixed at the optimal rate.

[0024] 5. The gas detection area can monitor the amount of methane produced during the anaerobic degradation process in real time, and the liquid detection area can directly detect the content of specific functional groups during the reaction process through a high-speed camera and a Raman spectroscopy system.

[0025] 6. Under the action of dielectrophoretic force, the electrophoretic separation device can purify the bacterial solution and discharge it through the microchannel, simplifying the filtration measures in the biological detection process. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 The overall structural diagram of the present invention is provided;

[0027] Figure 2 is a structural schematic diagram of a liquid mixing chamber;

[0028] Figure 3 It is a structural schematic diagram of the anaerobic degradation reaction chamber;

[0029] Figure 4 It is a schematic diagram of the structure of the storage device;

[0030] Figure 5 It is a schematic diagram of the structure of the gas detection device. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0032] The present invention provides a microfluidic chip suitable for coal microbial gasification, such as Figure 1 As shown, including the raw material mixing system, the degradation reaction system, the gas detection system, the biological separation system, the operation of each device in the chip, the result monitoring, the data regulation, etc. are all controlled by the intelligent control system. Under the action of the power system, the raw material mixing system is connected to the degradation reaction system, the degradation reaction system is connected to the gas and liquid detection systems respectively, the gas and liquid detection systems are connected to the biological separation system, and the operation of the entire device, the variable parameter adjustment, the gas and liquid recovery, etc. are operated under the intelligent control system. The present invention has the advantages of strong controllability, high convenience, easy operation, etc. At the same time, it can explore the coal microbial gasification process from the essence from a microscopic perspective, and has broad application prospects.

[0033] The microfluidic chip plans the area occupied by each part, and the chip is placed in a dark box. The substrate is a hard insulating sheet, including one of a ceramic substrate, a silicon wafer, a quartz substrate, a glass substrate, and a hard polymer substrate.

[0034] The raw material mixing system includes multiple injection ports, liquid mixing channels and liquid mixing chambers. The injection ports are respectively fed with nutrient solution, bacterial solution and ion solution. The liquid mixing channels mix different liquids in different proportions to obtain multiple mixed liquids, which can meet the growth needs of the bacterial community and can be distinguished according to experimental requirements. The mixed liquid in the raw material mixing system is a mixed liquid of organic nutrient solution and bacterial solution including methanogenic archaea, clostridium and other microorganisms.

[0035] like Figure 2 As shown, the raw material mixing system includes a liquid inlet branch pipe 1 and a bacteria inlet branch pipe 2. A suspended matter filter 5 and a valve 1 3 are provided at the liquid inlet of the liquid inlet branch pipe 1. A valve 2 4 is provided at the liquid inlet of the bacteria inlet branch pipe 2. The liquid inlet branch pipe 1 and the bacteria inlet branch pipe 2 are respectively connected to a liquid inlet main channel 6. The outlet of the liquid inlet main channel 6 is connected to a liquid mixing chamber 7. A valve 3 8 is provided at the liquid outlet of the liquid mixing chamber 7. The degradation reaction system is connected to the liquid outlet channel of the liquid mixing chamber 7 through the sampling area.

[0036] The degradation reaction system includes a sample injection area, which includes multiple independent sample injection modules and sample output modules. The sample injection module includes a mixed liquid inlet, a mixed liquid main channel, a mixed liquid branch channel, and multiple anaerobic degradation reaction chambers connected to the mixed liquid branch channel. Each of the anaerobic degradation reaction chambers includes a liquid inlet channel, which is used to inject different solutions to change the reaction conditions in the reaction chamber to meet the experimental requirements. The premixing channel separates the mixed solution from the bacterial solution in the reaction chamber through an isolation device. The sample output module includes a sample output main channel, a sample output branch channel, and a liquid output connection area.

[0037] Specifically, the degradation reaction system consists of a sample inlet area, an anaerobic degradation reaction chamber, a temperature control circuit area, and a sample outlet area.

[0038] The sampling area includes a mixed liquid main channel 9 connected to the liquid outlet channel of the liquid mixing chamber 7, the mixed liquid main channel 9 is connected to the liquid mixing chamber 7, and a plurality of anaerobic degradation reaction chambers 17 are respectively connected to the mixed liquid main channel 9 through sampling branch channels 10. At the same time, the sampling branch channels 10 are connected to the liquid inlet channel 11, and the liquid inlet channel 11 is used to inject different ion solutions. A valve 4 12 is provided at the liquid outlet of the liquid inlet channel 11, and a valve 5 13 is provided on the sampling branch channel 10.

[0039] The sample outlet area includes a sample outlet main channel 16 and a sample outlet branch channel 14, and a plurality of anaerobic degradation reaction chambers 17 are connected to the sample outlet main channel 16 through the sample outlet branch channels 14, wherein a valve 6 15 is provided on the sample outlet branch channel 14.

[0040] The anaerobic degradation reaction chamber 17 is used for the microbial gasification process of coal. During the reaction process, the anaerobic degradation reaction chamber 17 is filled with liquid to create an anaerobic environment. The anaerobic degradation reaction chamber 17 is divided into a grid area of ​​100um×100um×100um in length×width×height. Both ends of the anaerobic degradation reaction chamber 17 are connected to the sample inlet branch channel 10 and the sample outlet branch channel 14. Figure 3 As shown, it is a pipeline structure around an anaerobic degradation reaction chamber.

[0041] The temperature control circuit area includes thermoelectric materials and a control area. Laser etching is used to process the grooves for placing thermoelectric materials, through holes 18 are set around the anaerobic degradation reaction chamber, and a circuit layer 19 of thermoelectric materials is printed on the front of the temperature control circuit area, and a circuit layer 20 of thermoelectric materials is printed on the back, and copper is plated at the through holes 18 to form an alternating circuit structure. Specifically, the circuit layer 19 and the circuit layer 2 20 are alternately connected to realize a series circuit, and the series circuit is connected to an external temperature controller 41. NTC thermoelectric probes 21 are printed around the anaerobic degradation reaction chamber. The thermoelectric probes use thermocouples, and the probes are connected to a temperature collector 22 to monitor the temperature of the reaction chamber. The end of the temperature control circuit is connected to the temperature controller 41 to control the temperature of the reaction chamber.

[0042] When current flows into the N-type semiconductor and flows out of the P-type semiconductor, the carriers at the cold end diffuse to the hot end, the temperature around the circuit decreases, and the temperature of the anaerobic degradation reaction chamber decreases accordingly. When the current is reversed, the temperature of the anaerobic degradation reaction chamber increases accordingly.

[0043] The gas and liquid detection system includes a storage device 34, a gas detection device 30, a liquid detection device 33, and an anaerobic device 40. The storage device 34 is connected to the gas detection device 30 through an air inlet channel 31 and is connected to the liquid detection device 33 through a liquid inlet channel 32. Figure 4 As shown, the anaerobic device 40 is used to create an anaerobic environment, driving nitrogen gas to enter the gas storage device 37 through the air inlet channel 39. After the nitrogen gas is mixed with the gas produced by the anaerobic degradation reaction chamber, it enters the gas detection device through the air inlet channel 31. The gas is gathered in the upper part of the storage device 34, that is, the gas storage device 37, and the solution is gathered in the lower part of the storage device 34, that is, the liquid storage device 38.

[0044] The storage device 34 is connected to the gas detection device 30 through the air inlet channel 31, and is connected to the liquid detection device 33 through the liquid inlet channel 32. Specifically, the exhaust channel 36 and the liquid inlet channel 32 are connected to the liquid storage device 38, and the air inlet channel 31 is connected to the gas storage device 37. A partition is provided between the gas storage device 37 and the liquid storage device 38 for separating the space. A main inert gas inlet 35 is provided on the partition for gas circulation between the liquid storage device 38 and the gas storage device 37.

[0045] like Figure 5 As shown, the gas detection device 30 can detect the methane content in the gas produced by anaerobic degradation. The gas detection device 30 includes a substrate, which is made of PMMA. The substrate contains a microchannel 23. A certain concentration of polyvinyl alcohol (PVA) is dispersed in water, and the suspension is dripped onto the heated channel wall to promote its uniform distribution. After it is naturally dried, a polyvinyl alcohol (PVA) coating 26 can be formed on the inner wall of the channel. The thickness of the obtained coating is 3 to 5 microns. One end of the substrate is a gas inlet 27, and the other end is a microcavity 28. A metal oxide semiconductor (MOS) sensor based on redox reaction is installed in the microcavity. The sensor consists of an integrated heater 24, staggered platinum electrodes 25 with metal oxide semiconductors, and a monitoring device 29.

[0046] Metal oxide semiconductors can selectively adsorb methane. The resistance of the MOS sensor decreases when it reacts with reducing gases, and increases when it reacts with oxidizing gases. Due to the interaction with the channel coating, different gases will produce different time responses, which introduces monitoring capabilities to the MOS sensor.

[0047] The biological separation system 42 includes an electrophoresis area, a collection area, an electrode cavity 43, an excitation circuit module 46, a biological storage area 48, and a waste liquid area 50; the electrode cavity 43 and the excitation circuit module 46 are located in the electrophoresis area, and the biological storage area 48 and the waste liquid area 50 are located in the collection area;

[0048] A microelectrode positive electrode 44 and a microelectrode negative electrode 45 are arranged on the electrode cavity 43 along the direction of liquid flow. The microelectrode positive electrode 44 and the microelectrode negative electrode 45 are staggered in positive and negative distribution. The excitation circuit module 46 is connected to the microelectrode positive electrode 44 and the microelectrode negative electrode 45 through wires to form a loop; the electrode cavity 43 is used to generate a non-uniform electric field under the action of positive and negative charges. The electrode cavity contains liquid produced by anaerobic degradation reaction, in which different particles have different charges and different movement trajectories. The waste liquid with impurities and the bacterial liquid enter different storage devices through different sorting channels.

[0049] The electrode cavity 43 is connected to the storage device 34 through the discharge channel 36. The discharge channel 36 is provided with a valve 7 39. A microchannel with a radius of 100 um is made inside the electrode cavity 43 by laser etching, which is used to separate the bacterial solution from impurities. The outlet at the rear end of the electrode cavity is divided into two paths, which are connected to the biological storage area 48 through the first sorting sample channel 47. The first sorting sample channel 47 is close to the microelectrode positive electrode 44 and the microelectrode negative electrode 45, and is connected to the waste liquid area 50 through the second sorting sample channel 49. The microelectrode positive electrode 44 and the microelectrode negative electrode 45 generate a non-uniform electric field, which adsorbs the coal powder in the solution in the electrode cavity 43, gathers the coal powder to the microelectrode positive electrode 44 and the microelectrode negative electrode 45, and squeezes the bacteria in the solution to the opposite side. The coal powder thus enters the second sorting sample channel 49, and the bacteria enter the first sorting sample channel 48, thereby obtaining a liquid with impurities such as coal powder and a bacterial solution separated after electrophoresis convergence.

[0050] Principle: In a non-uniform electric field, neutral particles are polarized in the electric field, and the charge distribution on the particle surface changes. The non-uniformity of the electric field and the difference in the charge of the particles themselves cause different particles to be subjected to different Coulomb forces at different positions. At the same time, the particles in the microchannel are affected by the fluid force, which is controlled by the flow rate. Affected by the fluid force and the dielectrophoretic force, the motion trajectories of different particles in the non-uniform electric field will change, thereby achieving the purpose of separation.

[0051] The working process of the present invention is as follows: open valve 1 3, inject nutrient solution required for bacterial survival through liquid inlet branch pipe 1, and filter miscellaneous bacteria in the nutrient solution through suspended matter filter 5; open valve 2 4, inject bacterial solution required for anaerobic degradation through bacteria inlet branch pipe 2; the two are preliminarily mixed in liquid inlet main channel 6, and finally enter liquid mixing chamber 7 for full mixing, and preliminarily culture to clarify bacterial activity;

[0052] Add 200 mesh coal powder to the grid of the anaerobic degradation reaction chamber, and try to make each grid contain only one particle of coal powder and evenly distributed; open valve three 8, the liquid enters different sampling branch channels 10 through the main channel 9 of the mixed liquid, and finally injects into the grid of the anaerobic degradation reaction chamber 17, so that the liquid fills the grid and creates an anaerobic environment for the reaction chamber; adjust the temperature of the thermoelectric material through the temperature controller 41, so that the temperature inside the reaction chamber is 37°C, which meets the environmental requirements of bacterial degradation. The temperature inside the reaction chamber is collected by the temperature collector through the thermoelectric probe to achieve the purpose of real-time monitoring of temperature changes 24 hours a day.

[0053] The anaerobic device 40 is adjusted to slowly and uniformly input nitrogen into the gas storage device 37 through the air inlet channel 39, so that the gas produced by coal degradation flows with the input nitrogen and enters the gas detection device 30 through the air outlet channel 31, and then enters the microchannel 23 of the gas detection device substrate. Under the influence of the polyvinyl alcohol (PVA) coating 26, the carbon dioxide produced by the anaerobic degradation reaction is absorbed, and only methane remains as the reducing gas in the channel; under the influence of the integrated heater 24, a temperature difference is formed inside and outside the microcavity, and the treated gas enters the microcavity and contacts with the platinum electrode 25 containing a metal oxide semiconductor, and a redox reaction occurs, the semiconductor resistance changes, the electrical signal changes, and the monitoring device 29 collects data, based on which the response curve of the diffusion rate and concentration of methane in the gas with the change of the semiconductor resistance can be calculated;

[0054] After the reaction is finished, sterile water is injected at a certain flow rate through the liquid inlet branch pipe 1. The liquid produced in the anaerobic degradation reaction chamber flows with the injected sterile water, and enters the liquid storage device 38 through the sample outlet main channel 16, and then enters the liquid detection device 33 through the liquid outlet channel 32. The content of each organic matter in the liquid is analyzed by a high-speed camera and a Raman spectroscopy system device, etc., combined with a liquid flow calculation formula. In order to separate the bacterial flora and impurities such as coal powder in the solution, the liquid produced by the anaerobic degradation reaction is transported to the electrode cavity through the liquid outlet of the storage device, and then the discharge channel 36 is closed and the excitation circuit module 46 is opened to generate a non-uniform electric field in the electrode cavity 43. Under the influence of the dielectrophoretic force generated by its own charge, the coal powder with a diameter of 75um flows from the second sorting sample outlet channel 49 into the waste liquid area 50, corresponding to the strong electric field area, and the bacteria and archaea with a diameter of 5-10um flow from the first sorting sample outlet channel 47 into the biological storage area 48, corresponding to the weak electric field area. The bacterial liquids from 48 locations in the biological storage area can be collected, filtered, and used to sample and test the microbial communities in the process of coal biodegradation.

[0055] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A microfluidic chip suitable for coal microbial gasification, characterized in that: It includes a raw material mixing system, a degradation reaction system, a gas and liquid detection system, and a biological separation system arranged on a chip substrate, wherein the raw material mixing system is connected to the degradation reaction system through a pipeline, the degradation reaction system is connected to the gas and liquid detection system, and the gas detection system is connected to the biological separation system; The raw material mixing system comprises a liquid mixing chamber (7), on which a liquid inlet branch pipe (1) and a bacteria inlet branch pipe (2) are provided, and the liquid inlet branch pipe (1) and the bacteria inlet branch pipe (2) are respectively connected to the liquid mixing chamber (7) through a liquid inlet main channel (6); The degradation reaction system comprises a mixed liquid main channel (9), a sample outlet main channel (16) and a plurality of anaerobic degradation reaction chambers (17); the mixed liquid main channel (9) is communicated with the liquid mixing chamber (7); the plurality of anaerobic degradation reaction chambers (17) are respectively connected to the mixed liquid main channel (9) via sample outlet branch channels (10); the plurality of anaerobic degradation reaction chambers (17) are respectively connected to the sample outlet main channel (16) via sample outlet branch channels (14); the sample outlet branch channels (10) are further provided with liquid inlet channels (11) for feeding different ion solutions into the anaerobic degradation reaction chambers (17); the anaerobic degradation reaction chambers (17) can be selectively used, and the other anaerobic degradation reaction chambers (17) are closed; The gas and liquid detection system comprises a storage device (34), a gas detection device (30), a liquid detection device (33), and an anaerobic device (40); the storage device (34) is connected to the sample outlet main channel (16); the storage device (34) is connected to the gas detection device (30) through an air inlet channel (31), is connected to the liquid detection device (33) through a liquid inlet channel (32), and is connected to the anaerobic device (40) through an air inlet channel (39); The biological separation system (42) comprises an electrode chamber (43), an excitation circuit module (46), a biological storage area (48), and a waste liquid area (50). A microelectrode positive electrode (44) and a microelectrode negative electrode (45) are arranged on the electrode chamber (43) along the liquid flow direction. The excitation circuit module (46) is connected to the microelectrode positive electrode (44) and the microelectrode negative electrode (45) through electric wires to form a loop. The electrode chamber (43) is connected to the storage device (34) through a discharge channel (36), and is connected to the biological storage area (48) through a first sorting sample channel (47). 8), the first sorting sample channel (47) is close to the side of the microelectrode positive electrode (44) and the microelectrode negative electrode (45), and is connected to the waste liquid area (50) through the second sorting sample channel (49). The microelectrode positive electrode (44) and the microelectrode negative electrode (45) generate a non-uniform electric field, adsorbing the coal powder in the solution in the electrode cavity (43), and gathering the coal powder to the side of the microelectrode positive electrode (44) and the microelectrode negative electrode (45), and expelling the bacteria in the solution to the opposite side, so that the bacteria enter the first sorting sample channel (47), and the coal powder enters the second sorting sample channel (49).

2. A microfluidic chip suitable for coal microbial gasification according to claim 1, characterized in that: The anaerobic degradation reaction chamber (17) is provided with an NTC thermoelectric probe (21), which adopts a thermocouple and is connected to an external temperature collector (22).

3. A microfluidic chip suitable for coal microbial gasification according to claim 2, characterized in that: A plurality of through holes (18) are provided around the plurality of anaerobic degradation reaction chambers (17); a plurality of circuit layers (19) are printed on the front side of the chip substrate, and a plurality of circuit layers (20) are printed on the back side; the circuit layers (19) and the circuit layers (20) are electrically connected via copper plating at the through holes (18); the circuit layers (19) and the circuit layers (20) are alternately connected to realize a series circuit; and the series circuit is connected to an external temperature controller (41).

4. The microfluidic chip suitable for coal microbial gasification according to claim 1, characterized in that: The storage device (34) comprises a lower layer liquid storage device (38) for storing a solution and an upper layer gas storage device (37) for storing a gas, the discharge channel (36) and the liquid inlet channel (32) are connected to the liquid storage device (38), and the gas inlet channel (31) is connected to the gas storage device (37).

5. The microfluidic chip suitable for coal microbial gasification according to claim 4, characterized in that: A partition is provided between the gas storage device (37) and the liquid storage device (38), and a main inlet (35) for inert gas is provided on the partition.

6. The microfluidic chip suitable for coal microbial gasification according to claim 4, characterized in that: The gas storage device (37) is connected to the anaerobic device (40) via an air inlet passage (39).

7. A microfluidic chip suitable for coal microbial gasification according to claim 1 or 4, characterized in that: The gas detection device (30) comprises a substrate, a microchannel (23) is provided on the surface of the substrate, a polyvinyl alcohol coating (26) is provided on the inner wall of the microchannel (23), one end of the microchannel (23) is a gas inlet (27), and the other end is a microcavity (28) for detection, a metal oxide semiconductor sensor based on redox reaction is installed in the microcavity (28), and the metal oxide semiconductor sensor comprises an integrated heater (24), staggered platinum electrodes (25) with metal oxide semiconductors, and a monitoring device (29).

8. The microfluidic chip suitable for coal microbial gasification according to claim 1, characterized in that: The liquid inlet branch pipe (1) is provided with a suspended matter filter (5) and a valve one (3), the bacteria inlet branch pipe (2) is provided with a valve two (4), a valve three (8) is provided between the mixed liquid main channel (9) and the liquid mixing chamber (7), the liquid inlet channel (11) is provided with a valve four (12), the sample injection branch channel (10) is provided with a valve five (13), the sample outlet branch channel (14) is provided with a valve six (15), and the discharge channel (36) is provided with a valve seven (39).

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