Stem cell culture equipment

Abstract

The invention discloses stem cell culture equipment, and relates to the technical field of cell culture equipment, the stem cell culture equipment comprises a culture box, a rotating disc and a culture dish, horizontal plates are arranged in the middle of the culture box at equal intervals, and the middle of each horizontal plate is connected with the rotating disc through a bearing. The stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, a limiting ring and a circular track are separated, a conical block drives a tray to push culture dishes to be rapidly taken and placed, a rotating disc rotating slowly facilitates personnel to take or place the culture dishes one by one at the position close to an opening of a culture box, the limiting ring and the circular track are in matched butt joint, and the operation is convenient. The extrusion part elastically extrudes the culture dish on the tray when reversely moving and overturning, and meanwhile, the traction balance wheel is propped against the upper edge of a sealing end cover of the culture dish, so that the culture equipment can prevent the culture dish which is rotationally placed from shaking and shifting, and can be used for lifting and ejecting all the culture dishes at one time when the culture dish is taken out; and the operation of manually placing the culture dishes can also be reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture equipment, in particular to a stem cell culture equipment. Background Art

[0002] Stem cells are a type of multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into multiple functional cells. The development and progress of modern medicine is inseparable from the cultivation of stem cells. The cultivation of stem cells is generally carried out by placing stem cells in a culture vessel and then placing them in an incubator. Stem cell culture equipment is a key tool for stem cell research and application. They can provide an environment suitable for stem cell growth and differentiation.

[0003] According to the Chinese patent with the announcement number CN112342141A, a stem cell culture device is disclosed, which places a culture vessel inside a fixed placement tank, and when the second turntable rotates, the culture vessel also rotates, so that the culture fluid inside the culture vessel is in a circular motion state, preventing the culture fluid from solidifying when it is static, so that the stem cells can remain active for a long time, thereby increasing the culture time; According to the Chinese patent with the announcement number CN217628423U, a stem cell culture device is disclosed, which can achieve the purpose of high working efficiency and solve the problem of low working efficiency of general stem cell culture devices. When using the stem cell culture device, it is convenient for users to sample stem cells and save the user's operation time, thereby improving the practicality of the stem cell culture device; When the above technical solution is in use, the culture vessel is restricted and fixed in a specified position by using a fixing component. This fixing method cannot restrict the sealing end cover of the culture vessel, and when taking and placing the culture vessel, personnel need to operate the docking state of the fixing component and the culture vessel one by one. Such operation cannot tighten or take multiple culture vessels at one time. Summary of the invention

[0004] The object of the present invention is to provide a stem cell culture device to solve the problems raised by the above background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a stem cell culture device, comprising a culture box, a turntable and a culture dish, wherein a horizontal plate is equidistantly arranged in the middle of the culture box, a turntable is connected to the middle of the horizontal plate through a bearing, a ring rail is fixed to the lower side of the turntable, and tooth grooves are equidistantly provided on the outer edge of the lower side of the ring rail, a switching mechanism is provided on the lower side of the turntable, the switching mechanism comprises an operating ring sliding with the lower side of the ring rail, a lever is fixed to the outer wall surface of the operating ring, an adjusting disk is rotatably connected to the bottom surface of the turntable, a limiting ring is sleeved on the outer side of the operating ring, a ramp groove plugged with the lever is provided on the surface of the limiting ring, and a clamping block is provided on the upper edge of the limiting ring near the tooth groove; An adjusting cavity is provided on the upper surface of the turntable, and a driving disk is slidably connected in the adjusting cavity, a limited seat is provided above the adjusting cavity, a sliding groove is provided on the lower surface of the limiting seat, and a latch tooth b is provided on one side of the inner wall of the sliding groove, an anti-deviation mechanism is provided inside the limiting seat, and the anti-deviation mechanism includes a transmission gear docked with the driving disk, a threaded rod is fixed on the upper side of the transmission gear, and a cone block is threadedly connected to the outer side of the threaded rod, a sliding rod is slidably connected to the outer conical surface of the cone block, a rotating part is provided at one end of the sliding rod, the rotating part consists of a direction adjustment gear and a connecting pin fixed to the upper end of the direction adjustment gear, and an extrusion part is sleeved on the outer side of the connecting pin, a tray is fixed on the top of the cone block, and a culture dish for stem cell culture is provided on the upper side of the tray.

[0006] Preferably, a motor is installed on the top of the incubator, and a geared disc rod penetrating the incubator is provided at the output end of the motor, the geared disc rod is composed of a rotating rod body and a geared disc, and slots are provided at equal angles on the outer peripheral side surface of the turntable, and the slots are meshingly connected with the geared disc.

[0007] Preferably, the surface of the adjusting disk is provided with arc grooves eccentrically arranged with equal angles to the adjusting disk, and the lower surface of the rotating disk is provided with a straight groove connected with the adjusting cavity.

[0008] Preferably, the driving disk is composed of a sliding disk and a traction hook, the traction hook of the driving disk extends through a straight groove and is connected to the arc groove on the surface of the adjustment disk, a rectangular groove is opened in the middle of the driving disk, and a latch tooth a that meshes with the transmission gear is equidistantly arranged on one side of the rectangular groove.

[0009] Preferably, an annular anti-deviation groove is provided on the outer side of the transmission gear, and a guide bar is provided on the side of the rectangular groove away from the latch tooth a, and the guide bar is slidably connected to the annular anti-deviation groove of the transmission gear.

[0010] Preferably, the ramp grooves on the surface of the limiting ring are distributed at equal angles, horizontal grooves are provided at the upper and lower ends of the ramp groove, and an elastic pin is inserted into the inner wall of the horizontal groove at the lower end of the ramp groove.

[0011] Preferably, a vertical guide opening is provided on the outer side surface of the operating ring, and a protrusion that slides with the vertical guide opening is provided on the inner wall of the limiting ring.

[0012] Preferably, a T-shaped groove for sliding with the slide rod is provided on the conical surface of the cone block, the direction-adjusting gear of the rotating member is meshed with the latching tooth b on the inner wall of the slide groove, an elastic block is provided on the outer surface of the extrusion member, the elastic block of the extrusion member is a crescent-shaped structure, and the inner wall of the extrusion member is butted with the connecting pin on the rotating member by glue; The outer edge of the bottom of the cone block is movably connected with a gas spring rod, the upper end of the gas spring rod is rotationally connected to the balance wheel, and the lower end of the balance wheel is rotationally connected to the limit seat.

[0013] Preferably, a placing table is installed on the upper side of the turntable, and a through hole for limiting the installation position of the limiting seat is opened on the surface of the placing table.

[0014] Preferably, a sealed door is movably connected to one side of the incubator, and an observation window is provided in the middle of the sealed door. A thermostat for controlling the internal temperature of the incubator is installed on the outside of the incubator.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: the stem cell culture equipment is provided with a switching mechanism and an anti-deviation mechanism, the limiting ring is separated from the ring rail, the tray is driven by the cone block to push the culture dish for quick pick-up and placement, and the slowly rotating turntable is convenient for personnel to take or place the culture dishes one by one near the opening of the incubator, the limiting ring is matched and docked with the ring rail, so that the extrusion part elastically squeezes the culture dish on the tray when it moves in the reverse direction and flips, and at the same time, the traction balance wheel is pressed against the upper edge of the sealing end cover of the culture dish, ensuring that the culture equipment can prevent the rotating culture dish from shaking and deflecting, and can lift and eject all the culture dishes at one time when taking out the culture dishes, and can also reduce the manpower for placing the culture dishes. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the three-dimensional structure of the culture device of the present invention; Figure 2 A schematic diagram of the three-dimensional structure of a display table with the anti-deflection mechanism of the present invention opened; Figure 3 It is a schematic diagram of the three-dimensional structure of the adjusting disk tightening the driving disk of the present invention; Figure 4 It is a schematic diagram of the three-dimensional structure of the placing table with the anti-deviation mechanism tightened according to the present invention; Figure 5 It is a schematic diagram of the three-dimensional structure of the adjusting disk extruding the driving disk of the present invention; Figure 6 It is a schematic diagram of a three-dimensional explosion structure in which a rotating disk, a switching mechanism and an anti-deviation mechanism are linked in the present invention; Figure 7 It is a schematic diagram of the three-dimensional structure of the switching mechanism of the present invention; Figure 8 For the present invention Figure 7 A schematic diagram of the partially enlarged structure at center A; Fig. 9 It is a schematic diagram of a three-dimensional cross-section structure of the switching mechanism of the present invention; Fig.10 For the present invention Fig. 9 A schematic diagram of the partially enlarged structure at B in the middle; Fig.11 It is a schematic diagram of the three-dimensional cross-section structure of the anti-deviation mechanism of the present invention clamping the culture dish; Fig.12 It is a schematic diagram of the three-dimensional structure of the balance wheel with the anti-bias mechanism of the present invention; Fig.13 It is a schematic diagram of the three-dimensional structure of the anti-bias mechanism for tightening the balance wheel of the present invention.

[0017] In the figure: 1. incubator; 101. motor; 102. geared disc rod; 103. temperature controller; 2. horizontal plate; 3. turntable; 301. ring track; 302. adjustment chamber; 303. tooth groove; 4. display table; 5. switching mechanism; 501. operating ring; 502. lever; 503. adjustment disc; 504. limiting ring; 505. ramp channel; 506. elastic pin; 507. block; 508. drive disc; 509. tooth a; 6. limiting seat; 601. slide groove; 602. tooth b; 7. anti-bias mechanism; 701. transmission gear; 702. cone block; 703. tray; 704. slide rod; 705. rotating part; 706. extrusion part; 707. gas spring rod; 708. balance wheel; 8. culture dish. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] See also Figure 1-Figure 10The present invention provides a technical solution: a stem cell culture device, comprising a culture box 1, a turntable 3 and a culture dish 8, a horizontal plate 2 is equidistantly arranged in the middle of the culture box 1, a turntable 3 is connected to the middle of the horizontal plate 2 through a bearing, a motor 101 is installed on the top of the culture box 1, a geared disc rod 102 penetrating the culture box 1 is arranged at the output end of the motor 101, the geared disc rod 102 is composed of a rotating rod body and a geared disc, a card groove is provided at an equal angle on the outer peripheral surface of the turntable 3, and the card groove is meshed with the geared disc, when the motor 101 is started to drive the geared disc rod 102 to rotate, the geared disc on the geared disc rod 102 will mesh with the card groove on the outer peripheral side of the turntable 3 for transmission, thereby driving the turntable 3 to rotate slowly on the horizontal plate 2, so as to facilitate the culture dish 8 to follow the turntable 3 to rotate to perform a shaking operation of the stem cell culture solution; A ring rail 301 is fixed to the lower side of the turntable 3, and tooth grooves 303 are equidistantly provided on the outer edge of the lower side of the ring rail 301. A switching mechanism 5 is provided on the lower side of the turntable 3. The switching mechanism 5 includes an operating ring 501 that slides with the lower side of the ring rail 301. A lever 502 is fixed to the outer wall of the operating ring 501. An adjusting disk 503 is rotatably connected to the bottom surface of the turntable 3. An arc groove eccentrically arranged with the adjusting disk 503 is provided on the surface of the adjusting disk 503 at equal angles. A straight groove communicating with the adjusting cavity 302 is provided on the lower surface of the turntable 3. A limiting ring 504 is sleeved on the outer side of the operating ring 501. A ramp groove 505 is provided on the surface of the limiting ring 504 for plugging with the lever 502. A clamping block 507 is provided on the upper edge of the limiting ring 504 near the tooth groove 303. An adjusting cavity 302 is provided on the upper surface of the rotating disk 3, and a driving disk 508 is slidably connected in the adjusting cavity 302. A limiting seat 6 is provided above the adjusting cavity 302. A sliding groove 601 is provided on the lower surface of the limiting seat 6, and a latching tooth b602 is provided on one side of the inner wall of the sliding groove 601. An anti-bias mechanism 7 is provided inside the limiting seat 6. The anti-bias mechanism 7 includes a transmission gear 701 docked with the driving disk 508. A threaded rod is fixed on the upper side of the transmission gear 701, and a cone block 702 is threadedly connected to the outer side of the threaded rod. A sliding rod 704 is slidably connected to the outer conical surface of the cone block 702. A rotating member 705 is provided at one end of the sliding rod 704. The rotating member 705 consists of a direction-adjusting gear and a connecting pin fixed to the upper end of the direction-adjusting gear, and an extruding member 706 is sleeved on the outer side of the connecting pin. A tray 703 is fixed on the top of the cone block 702, and a culture dish 8 for stem cell culture is provided on the upper side of the tray 703. The driving disk 508 is fixedly composed of a sliding disk and a traction hook, the traction hook of the driving disk 508 extends through a straight groove and is connected to the arc groove on the surface of the adjusting disk 503, a rectangular groove is provided in the middle of the driving disk 508, and a latch tooth a509 meshing with the transmission gear 701 is equidistantly provided on one side of the rectangular groove, an annular anti-deflection groove is provided on the outer side of the transmission gear 701, and a guide bar is provided on the side of the rectangular groove away from the latch tooth a509, and the guide bar is slidably connected with the annular anti-deflection groove of the transmission gear 701, by providing the annular anti-deflection groove on the outer side of the transmission gear 701, when the adjusting disk 503 drives the driving disk 508 to reciprocate along the adjusting cavity 302, the driving disk 508 moves along the annular anti-deflection groove through the guide bar on one side of the rectangular groove, so as to limit the position of the transmission gear 701, prevent the transmission gear 701 from disengaging from the driving disk 508, and the driving disk 508 can ensure that the latch tooth a509 is stably meshed with the transmission gear 701 when moving; The ramp grooves 505 on the surface of the limiting ring 504 are distributed at equal angles, and horizontal notches are set at the upper and lower ends of the ramp grooves 505. An elastic pin 506 is inserted in the inner wall of the horizontal notch at the lower end of the ramp grooves 505. A plurality of levers 502 are arranged at equal angles on the outer peripheral side of the operating ring 501, and a reserved hole for sliding connection of the elastic pin 506 is opened in the inner wall of the horizontal notch at the lower end of the ramp grooves 505, and a spring is used between the elastic pin 506 and the reserved hole for elastic reset; by setting the ramp grooves 505 at equal angles on the surface of the limiting ring 504, it is convenient for the levers 502 with equal angles to cover the outer peripheral side of the operating ring 501 with a smaller spacing, and when the turntable 3 is continuously rotating, it is convenient The personnel quickly grab the lever 502 to switch the ring rail 301 and the limit ring 504 between separation and docking; when the lever 502 moves to the horizontal slot entrance end at the lower end of the ramp groove 505, the lever 502 will squeeze the elastic pin 506 and retract it into the reserved hole on the inner wall of the ramp groove 505. When the lever 502 is completely moved to the inside of the horizontal slot at the lower end of the ramp groove 505, the elastic pin 506 is elastically reset by the spring in the reserved hole, ensuring that the elastic pin 506 can block the lever 502 in the horizontal slot at the lower end of the ramp groove 505, preventing the lever 502 from being accidentally led out of the horizontal slot at the lower end of the ramp groove 505, causing the separation of the ring rail 301 and the limit ring 504.

[0020] See also Figure 11-13 A T-shaped groove for sliding with the slide bar 704 is provided on the conical surface of the cone block 702, the direction adjustment gear of the rotating member 705 is meshed with the clamping tooth b602 on the inner wall of the slide groove 601, and an elastic block is provided on the outer surface of the extrusion member 706. The elastic block of the extrusion member 706 is a crescent-shaped structure, and the inner wall of the extrusion member 706 is butt-jointed with the connecting pin on the rotating member 705 by glue; The outer edge of the bottom of the cone block 702 is movably connected with a gas spring rod 707, the upper end of the gas spring rod 707 is rotatably connected to the balance wheel 708, and the lower end of the balance wheel 708 is rotatably connected to the limit seat 6. The T-shaped groove on the conical surface of the cone block 702 is slidably connected with one end of the slide bar 704 to ensure that the cone block 702 can drive the slide bar 704 to be pulled or pushed in the horizontal position through the inclined T-shaped groove when moving up and down, and ensure that the rotating member 705 at one end of the slide bar 704 can achieve synchronous movement; A placing table 4 is installed on the upper side of the turntable 3. A through hole is opened on the surface of the placing table 4 for limiting the installation position of the limit seat 6. By setting the placing table 4 and the through hole, the limit seat 6 can be installed in a hidden manner, and the upper edge of the limit seat 6 is ensured to be flush with the upper surface of the placing table 4, which can facilitate the cleaning of the placing table 4.

[0021] When the lever 502 on the outside of the control operation ring 501 is connected with the horizontal notch at the upper end of the ramp groove 505, the lever 502 drives the limit ring 504 to move downward, so that the block 507 on the upper edge of the limit ring 504 is away from the tooth groove 303 on the lower edge of the ring track 301. At this time, the limit ring 504 and the ring track 301 are in a separated state, and the traction hook at one end of the drive disk 508 is connected with the end of the eccentric groove close to the center of the surface of the adjustment disk 503. At this time, the traction hook drives the drive disk 508 to move along the adjustment cavity 302 toward the center position of the surface of the rotating disk 3. Therefore, the drive disk 508 will be positively meshed with the transmission gear 701 through the tooth a509 in the rectangular groove in the middle of the drive disk 508, so that the rotating transmission gear 701 is positively threaded with the cone block 702 through the threaded rod, so that the cone block 702 drives the tray 703 to move upward, and the cone block 702 will pass through the outer cone surface when moving upward. The T-slot squeezes the slide bar 704 to move away from the tray 703, so that the slide bar 704 pushes the rotating member 705 and the extruding member 706 away from the tray 703, and in the process of the rotating member 705 moving away from the tray 703, the direction adjustment gear of the rotating member 705 will positively mesh with the latching tooth b602 on the inner wall of the slide groove 601, thereby driving the rotating member 705 and the extruding member 706 to flip, ensuring that the elastic block of the extruding member 706 is away from the tray 703 when flipping; when the cone block 702 moves upward, the cone block 702 will squeeze the arm of the balance wheel 708 upward through the movably connected gas spring rod 707, so that the balance wheel 708 rotates in the limit seat 6 and abuts against the edge of the upper opening of the limit seat 6, so that it is convenient for personnel to place the culture dish 8 for stem cell culture in the limit seat 6, and at the same time, the tray 703 pushed upward can facilitate personnel to quickly take out the culture dish 8 from the limit seat 6; When the limiting ring 504 of the switching mechanism 5 is in a separated state from the ring rail 301, the geared disc rod 102 is driven to rotate by starting the motor 101, and the geared disc on the geared disc rod 102 is meshed with the slot on the outer peripheral side of the turntable 3 for transmission, so that the turntable 3 rotates slowly on the horizontal plate 2 through the bearing, and the turntable 3 pushes the adjusting disc 503 and the operating ring 501 to rotate together through the traction hook at one end of the driving disc 508 when rotating. When the turntable 3 rotates slowly and the rotating member 705, the extruding member 706 and the balance wheel 708 are all away from the tray 703, it is convenient for personnel to take out the culture dishes 8 one by one from the limiting seat 6 near the opening of the incubator 1, or it is convenient for personnel to place the culture dishes 8 one by one into the limiting seat 6 near the opening of the incubator 1; After the culture dish 8 is placed, the lever 502 on the outside of the operating ring 501 is quickly rotated so that the lever 502 drives the operating ring 501 and the ring rail 301 to slide relative to each other, so that the lever 502 moves from the horizontal notch at the upper end of the ramp groove 505 to the horizontal notch at the lower end of the ramp groove 505, and the lever 502 drives the limiting ring 504 to move upward, so that the block 507 on the upper edge of the limiting ring 504 is engaged with the tooth groove 303 on the lower edge of the ring rail 301. At this time, the limiting ring 504 and the ring rail 301 are in a mating state, and the operating ring 501 drives the adjusting plate The rotation of 503 will squeeze the traction hook at one end of the driving disk 508 through the eccentric groove, so that the traction hook at one end of the driving disk 508 abuts against the eccentric groove on the outer periphery of the surface of the adjusting disk 503. At this time, the traction hook will drive the driving disk 508 to move along the adjusting cavity 302 to the outer periphery of the surface of the rotating disk 3. Therefore, the driving disk 508 will reversely mesh with the transmission gear 701 through the tooth a509 in the rectangular groove in the middle of the driving disk 508, so that the rotating transmission gear 701 performs reverse thread transmission with the cone block 702 through the threaded rod, so that the cone block 702 drives the tray 703 to move downward, and the downward moving cone block 702 will pull the slide rod 704 and drives the rotating member 705 and the extruding member 706 to approach the tray 703; when the rotating member 705 moves to a position close to the tray 703, the direction adjustment gear of the rotating member 705 will mesh with the latching tooth b602 on the inner wall of the slide groove 601 in the opposite direction, thereby driving the rotating member 705 and the extruding member 706 to flip in the opposite direction, ensuring that the elastic block of the extruding member 706 is close to the tray 703 when flipping, and the rotating extruding member 706 elastically squeezes the outer wall surface of the culture dish 8 through the crescent-shaped elastic block, which is convenient for adapting to culture dishes 8 of different sizes, and multiple groups of extruding members 706 are arranged on the periphery of the cone block 702 , ensuring that the culture dish 8 can be stably clamped in all directions; while the cone block 702 moves downward, the cone block 702 will pull the arm of the balance wheel 708 to deflect downward through the movably connected gas spring rod 707, so that the balance wheel 708 will press against the upper edge of the sealing end cover of the culture dish 8 when rotating in the limiting seat 6, so as to facilitate the positioning and clamping of the culture dish 8 for stem cell culture in the limiting seat 6. When the turntable 3 rotates, it can drive the culture dish 8 in the limiting seat 6 to rotate in a circle, so as to facilitate the continuous shaking of the culture solution in the culture dish 8, ensuring that the stem cells can maintain long-term activity during cultivation; When the limiting ring 504 of the switching mechanism 5 is in a mating state with the ring rail 301, the rotation of the turntable 3 driving the ring rail 301 will drive the switching mechanism 5 to rotate together. At this time, the adjusting disk 503 will limit the position of the driving disk 508 in the adjusting cavity 302. By maintaining the continuous rotation of the motor 101, the turntable 3 and the placing table 4 can rotate slowly in the incubator 1. The switching mechanism 5 can then drive the anti-deviation mechanism 7, thereby preventing the culture dish 8 from shaking and deviating when being placed, and lifting and ejecting the culture dish 8 when taking it out, thereby reducing the manpower required to place the culture dish 8.

[0022] See also Figure 5 and Fig. 9 A vertical guide opening is provided on the outer side surface of the operating ring 501, and a protrusion that slides with the vertical guide opening is provided on the inner wall of the limit ring 504. By providing the vertical guide opening and the protrusion that slides in the vertical guide opening, it is ensured that when the operating ring 501 is driven forward and reverse, the limit ring 504 can be driven to slide up and down longitudinally, and at the same time, the operating ring 501 can synchronously drive the limit ring 504 to rotate together when it rotates in a circle.

[0023] See also Figure 1 A sealed door is movably connected to one side of the incubator 1, and an observation window is provided in the middle of the sealed door. A thermostat 103 for controlling the internal temperature of the incubator 1 is installed on the outside of the incubator 1. When the sealed door on one side of the incubator 1 is closed, the observation window in the middle of the sealed door allows personnel to observe the general change state of the culture in the culture dish 8 from the outside of the incubator 1, and the temperature in the incubator 1 can be regulated by adjusting the thermostat 103 to ensure that the stem cell culture has suitable temperature conditions.

[0024] In summary, the culture dish 8 is placed in the limiting seat 6, and the outer wall of the culture dish 8 is clamped by the anti-deviation mechanism 7 and the outer edge of the sealing end cover is pressed against the limiting position to ensure that the culture dish 8 is stably limited. The temperature in the incubator 1 is regulated by the temperature controller 103 to ensure that the stem cell culture has suitable temperature conditions, and then the motor 101 is started to drive the gear rod 102 to engage with the turntable 3 for transmission, thereby driving the turntable 3 to rotate slowly on the horizontal plate 2, so as to facilitate the culture dish 8 to follow the rotation of the turntable 3 to shake the stem cell culture medium, thereby ensuring that the stem cells can be efficiently cultured in the incubator 1. The contents not described in detail in this specification belong to the existing technology known to professional and technical personnel in this field.

[0025] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A stem cell culture device, comprising a culture box (1), a turntable (3) and a culture dish (8), characterized in that: A horizontal plate (2) is equidistantly arranged in the middle of the incubator (1); a turntable (3) is connected to the middle of the horizontal plate (2) via a bearing; a ring rail (301) is fixed to the lower side of the turntable (3); and tooth grooves (303) are equidistantly provided on the outer edge of the lower side of the ring rail (301); a switching mechanism (5) is arranged on the lower side of the turntable (3); the switching mechanism (5) comprises an operating ring (501) that slides with the lower side of the ring rail (301); a lever (502) is fixed to the outer wall of the operating ring (501); an adjusting plate (503) is rotatably connected to the bottom surface of the turntable (3); a limiting ring (504) is sleeved on the outer side of the operating ring (501); a ramp groove (505) is provided on the surface of the limiting ring (504) that is plugged into the lever (502); and a clamping block (507) is arranged on the upper edge of the limiting ring (504) close to the tooth groove (303); The upper surface of the rotating disk (3) is provided with an adjustment cavity (302), and a driving disk (508) is slidably connected in the adjustment cavity (302). A limit seat (6) is provided above the adjustment cavity (302), a slide groove (601) is provided on the lower surface of the limit seat (6), and a latching tooth b (602) is provided on one side of the inner wall of the slide groove (601). An anti-deflection mechanism (7) is provided inside the limit seat (6), and the anti-deflection mechanism (7) includes a transmission gear (701) connected to the driving disk (508), and the transmission gear (701) A threaded rod is fixed on the upper side, and a conical block (702) is threadedly connected to the outer side of the threaded rod. A sliding rod (704) is slidably connected to the outer conical surface of the conical block (702). A rotating member (705) is provided at one end of the sliding rod (704). The rotating member (705) is composed of a direction-adjusting gear and a connecting pin fixed to the upper end of the direction-adjusting gear, and an extrusion member (706) is sleeved on the outer side of the connecting pin. A tray (703) is fixed to the top of the conical block (702), and a culture dish (8) for stem cell culture is provided on the upper side of the tray (703).

2. A stem cell culture device according to claim 1, characterized in that: A motor (101) is installed on the top of the incubator (1); a toothed disc rod (102) penetrating the incubator (1) is provided at the output end of the motor (101); the toothed disc rod (102) is composed of a rotating rod body and a toothed disc; the outer peripheral side surface of the rotating disc (3) is provided with slots at equal angles, and the slots are meshedly connected with the toothed disc.

3. A stem cell culture device according to claim 1, characterized in that: The surface of the adjusting disk (503) is provided with arc grooves eccentrically arranged with the adjusting disk (503) at equal angles, and the lower surface of the rotating disk (3) is provided with a straight groove connected with the adjusting cavity (302).

4. A stem cell culture device according to claim 3, characterized in that: The driving disk (508) is composed of a sliding disk and a traction hook, the traction hook of the driving disk (508) extends through a straight groove and is connected to an arc groove on the surface of the adjustment disk (503), a rectangular groove is provided in the middle of the driving disk (508), and a latching tooth a (509) meshing with a transmission gear (701) is equidistantly arranged on one side of the rectangular groove.

5. A stem cell culture device according to claim 4, characterized in that: An annular anti-deflection groove is provided on the outer side of the transmission gear (701), and a guide bar is provided on the side of the rectangular groove away from the latch tooth a (509), and the guide bar is slidably connected to the annular anti-deflection groove of the transmission gear (701).

6. The stem cell culture device according to claim 1, characterized in that: The inclined grooves (505) on the surface of the limiting ring (504) are distributed at equal angles, and horizontal grooves are provided at the upper and lower ends of the inclined groove (505). An elastic pin (506) is inserted into the inner wall of the horizontal groove at the lower end of the inclined groove (505).

7. The stem cell culture device according to claim 1, characterized in that: The outer side surface of the operating ring (501) is provided with a vertical guide opening, and the inner wall of the limiting ring (504) is provided with a protrusion that slides with the vertical guide opening.

8. The stem cell culture device according to claim 1, characterized in that: A T-shaped groove for sliding with the slide rod (704) is provided on the conical surface of the conical block (702); the direction-adjusting gear of the rotating member (705) meshes with the latching tooth b (602) on the inner wall of the slide groove (601); an elastic block is provided on the outer surface of the extrusion member (706); the elastic block of the extrusion member (706) is a crescent-shaped structure; the inner wall of the extrusion member (706) is butt-jointed with the connecting pin on the rotating member (705) by glue; The outer edge of the bottom of the cone block (702) is movably connected to a gas spring rod (707), the upper end of the gas spring rod (707) is rotationally connected to the balance wheel (708), and the lower end of the balance wheel (708) is rotationally connected to the limit seat (6).

9. A stem cell culture device according to claim 8, characterized in that: A placing table (4) is installed on the upper side of the turntable (3), and a through hole for limiting the installation position of the limiting seat (6) is provided on the surface of the placing table (4).

10. The stem cell culture device according to claim 1, characterized in that: A sealed door is movably connected to one side of the incubator (1), and an observation window is provided in the middle of the sealed door. A temperature controller (103) for controlling the internal temperature of the incubator (1) is installed on the outside of the incubator (1).

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