Visual cell culture container and device for cell experiment

Abstract

The invention provides a visual cell culture container and device for a cell experiment, and relates to the field of cell culture. The visual cell culture device for the cell experiment comprises an observation device, a culture device, a sealing device and a plurality of container bodies, the culture device is arranged at the top end of the observation device, the sealing device is arranged at the top end of the culture device, the container bodies are arranged in the culture device, and the observation device comprises a supporting plate. By arranging the observation device, the operation difficulty is simplified, the visualization effect of cell culture is improved, by arranging the culture device, the survival rate during cell culture is effectively improved, by arranging the sealing device, the device is effectively sealed in the culture process, the influence of the external environment on the container main body is avoided, and by arranging the container main body, the device is convenient to use. Enough light sources can be provided during observation, and meanwhile, loss of nutrient solution can be reduced by means of the arrangement of the convex points.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture, in particular to a visualized cell culture container and device for cell experiments. Background Art

[0002] Cell experiment visualization technology is an indispensable part of modern biomedical research. It allows researchers to observe and analyze the behavior and response of cells under different conditions in real time. The background technology of cell culture containers and devices includes multilayer cell culture containers, microfluidic chips, fluorescence microscopes, confocal microscopes, live cell imaging, Vitessce and other tools. These technologies are used to improve cell culture efficiency, achieve high-resolution three-dimensional imaging, observe the internal structure and dynamic processes of cells, explore multimodal and spatially resolved single-cell data, etc.

[0003] Although the prior art conducts certain research in the field of biology by culturing cells, when control experiments are required during the culture process, multiple culture dishes are needed, resulting in a huge waste of resources. Therefore, those skilled in the art provide a cell experiment visualization cell culture container and device to solve the problems raised in the above background technology. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a cell experiment visualization cell culture container and device, which solves the problem that it is inconvenient to observe the cell state during the cell culture process and can provide good light during the observation process.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: a cell experiment visualization cell culture device, comprising an observation device, a culture device, a sealing device and a plurality of container bodies, wherein the culture device is arranged at the top of the observation device, the sealing device is arranged at the top of the culture device, and the plurality of container bodies are arranged inside the culture device, the observation device comprises a support plate, a support frame is fixedly connected to the rear side of the top of the support plate, a third screw rod is rotatably connected to the inner side wall of the support frame, a third motor is fixedly connected to the top of the third screw rod, a slider is threadedly connected to the side wall of the third screw rod, the front end of the slider is fixedly connected to the second fixing frame, and the bottom end of the second fixing frame is fixedly connected to the front side. A first fixing frame, a microscopic camera is fixedly connected to the front end of the second fixing frame, a light-transmitting plate is rotatably connected to the other end of the first fixing frame, a supporting plate is fixedly connected to the front side of the top end of the supporting plate, a first motor is fixedly connected to one side of the first mounting frame, a first screw rod is rotatably connected to the inner side wall of the first mounting frame, a first limiting rod is respectively fixedly connected to the front and rear sides of the inner side wall of the first mounting frame, a second mounting frame is threadedly connected to the side wall of the first screw rod, a second motor is fixedly connected to the front end of the second mounting frame, a second screw rod is rotatably connected to the inner side wall of the second motor, second limiting rods are respectively fixedly connected to the two sides of the inner side wall of the second mounting frame, and a placement plate is threadedly connected to the side wall of the second screw rod.

[0008] Preferably: the culture device includes a shell, a turntable is rotatably connected to the middle of the inner wall of the shell, a temperature sensor is fixedly connected to one side of the inner wall of the shell, a humidity sensor is fixedly connected to the other side of the inner wall of the shell, an exhaust pipe is fixedly connected to the middle of the other side of the shell, the second connecting pipe is fixedly connected to the middle of one side of the shell, the other end of the first limit rod is fixedly connected to an air intake box, the bottom end of the inner wall of the air intake box is fixedly connected to a temperature control module, one side of the air intake box is fixedly connected to an air intake pipe, the exhaust pipe and the inner wall of the air intake pipe are respectively provided with filter screens, the inner wall of the air intake pipe is fixedly connected to an axial flow fan, the front and rear sides of the shell are respectively fixedly connected to an insulation shell, two connecting pipes are fixedly connected to the inner wall of the shell, a first connecting pipe is fixedly connected to the middle of the rear end of the insulation shell at the rear end of the shell, the other side of the first connecting pipe is fixedly connected to a constant temperature water tank, and the bottom end of the shell is fixedly connected to the top of the placement plate.

[0009] Preferably: the sealing device includes two third mounting brackets, a third limiting rod is fixedly connected to the inner side wall of the third mounting bracket at the rear end of the shell, a fourth screw is rotatably connected to the inner side wall of the third mounting bracket at the front end of the shell, a fourth motor is fixedly connected to one side of the third mounting bracket at the front end of the shell, the side wall of the fourth screw is threadedly connected to a cover plate, a telescopic rod is fixedly connected to the top of the cover plate, the movable end of the telescopic rod passes through the top of the cover plate and is fixedly connected to a sealing plate, and the two third mounting brackets are respectively fixedly connected to the upper position of the front end and the rear end of the shell.

[0010] Preferably: the output end of the first motor passes through the first mounting frame and is fixedly connected to one end of the first screw rod, the two side walls of the first limit rod are respectively slidably connected to the bottom end of the second mounting frame, the output end of the second motor passes through the front end of the second mounting frame and is fixedly connected to the front end of the second screw rod, the two side walls of the second limit rod are respectively slidably connected to the bottom end of the placement plate, and the output end of the third motor passes through the top end of the support frame and is fixedly connected to the top end of the third screw rod.

[0011] Preferably: the two connecting pipes respectively penetrate the inner wall of the shell and are connected to the interior of the two insulation shells, one end of the first connecting pipe is connected to the interior of the insulation shell, one end of the second connecting pipe is connected to the interior of the shell, and the other end of the second connecting pipe is connected to the interior of the air intake box.

[0012] Preferably: the output end of the fourth motor passes through one side of the third mounting frame and is fixedly connected to one end of the fourth screw rod, the rear end of the cover plate is slidably connected to the side wall of the third limiting rod, the sealing plate is slidably connected to the inner wall of the cover plate, and the cover plate and the top of the shell are adapted to each other.

[0013] Preferably: a cell experiment visualization cell culture container, comprising a plurality of container bodies, wherein the container bodies include culture dishes, the top of the culture dishes is provided with a plurality of grooves, the bottom ends of the inner side walls of the plurality of grooves are respectively provided with a plurality of protrusions, the bottom end of the culture dishes is fixedly connected with a working bin, the inner side walls of the plurality of working bins are provided with a plurality of lighting lamps, and the bottom ends of the plurality of working bins are fixedly connected with the top of a turntable.

[0014] Preferably, the bottom of the culture dish is a transparent glass structure, and the bottom ends of the inner side walls of the plurality of grooves are respectively provided with numbers.

[0015] Working principle: First, add the nutrient solution needed for cell culture into a plurality of grooves 401. After placing the cell samples, different culture adjustments can be set according to the different numbers in the grooves 401 to form a comparison. During the culture process, air is blown into the shell 203 through the axial flow fan 2015, and the environment in the shell 203 is detected through the temperature sensor 209 and the humidity sensor 2013. At the same time, constant temperature circulating water is added to the two insulation shells 204 through the constant temperature water tank 201, so that the culture environment of the cells remains unchanged. When it is necessary to observe the cells, first use the telescopic rod 307 to retract the sealing plate 302 from the shell 203, turn on the fourth motor 306, and use the fourth motor 306 to drive the sealing plate 302 to move the sealing plate 302 from the shell 203. The fourth screw rod 305 is driven to rotate, the sealing plate 302 is moved as a whole, the container body 4 is exposed as a whole, and the culture status of the cells is observed. Different apertures are selected by rotating the light-transmitting plate 1011, and the first screw rod 104 is driven to rotate by the first motor 103, and the second screw rod 106 is driven to rotate by the second motor 107. The groove 401 to be observed on the culture dish 402 can be moved to the bottom of the microscope camera 1012, and the third screw rod 1017 is driven to rotate by the third motor 1016 so that the microscope camera 1012 can find a clearer picture. When shooting, the lighting lamp 404 in the working chamber 403 can be turned on to supplement the light source for shooting, so that the clarity is improved.

[0016] (III) Beneficial effects

[0017] The present invention provides a cell culture container and device for visualizing cell experiments, which have the following beneficial effects:

[0018] 1. By setting up an observation device, different apertures are selected by rotating the light-transmitting plate, and at the same time, the first motor drives the first screw rod to rotate, and the second motor drives the second screw rod to rotate, so that the groove to be observed on the culture dish can be moved to the bottom of the microscope camera, and the third motor drives the third screw rod to rotate so that the microscope camera can find a clearer picture, and when shooting, the lighting in the working chamber can be turned on to supplement the light source for shooting, so that the clarity is improved, the part to be observed can be effectively adjusted, the operation difficulty is simplified, and the visualization effect of cell culture is improved.

[0019] 2. By setting up a culture device, during the culture process, air is blown into the shell through an axial flow fan, the environment in the shell is detected by a temperature sensor and a humidity sensor, and constant temperature circulating water is added to the two insulation shells through a constant temperature water tank, so that the culture environment of the cells remains unchanged, effectively improving the survival rate of the cells during culture.

[0020] 3. By setting up a sealing device, when it is necessary to observe the cells, first use the telescopic rod to retract, retract the sealing plate from the shell, turn on the fourth motor, use the fourth motor to drive the fourth screw to rotate, move the sealing plate as a whole, expose the container body as a whole, observe the culture status of the cells, effectively seal the device during the culture process, and prevent the external environment from affecting the container body.

[0021] 4. By setting up the container body, the nutrient solution needed for cell culture is added to several grooves. After the cell samples are placed, different culture adjustments can be set according to the different numbers in the grooves to form a control. Control variable experiments can be carried out during the culture process to improve the accuracy of the experimental results. Lighting lamps are set in the working chamber to provide sufficient light source for observation. At the same time, the setting of convex points can save the loss of nutrient solution. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the structure of the present invention;

[0023] Figure 2 It is a structural schematic diagram of the observation device of the present invention;

[0024] Figure 3 It is a schematic diagram of the structure of the culture device of the present invention;

[0025] Figure 4 It is a schematic diagram of the top view of the culture device of the present invention;

[0026] Figure 5 It is a structural schematic diagram of the container body of the present invention;

[0027] Figure 6 It is a schematic cross-sectional structural diagram of the container body of the present invention;

[0028] Figure 7 It is a side structural schematic diagram of the culture device of the present invention.

[0029] Among them, 1. observation device; 101. support plate; 102. first mounting frame; 103. first motor; 104. first screw rod; 105. first limit rod; 106. second screw rod; 107. second motor; 108. second mounting frame; 109. second limit rod; 1010. placement plate; 1011. light-transmitting plate; 1012. microscope camera; 1013. first fixing frame; 1014. second fixing frame; 1015. slider; 1016. third motor; 1017. third screw rod; 1018. supporting frame; 2. culture device; 201. constant temperature water tank; 202. first connecting pipe; 203. shell; 20 4. Insulation shell; 205. Second connecting pipe; 206. Air inlet box; 207. Filter; 208. Air inlet pipe; 209. Temperature sensor; 2010. Turntable; 2011. Exhaust pipe; 2012. Connecting pipe; 2013. Humidity sensor; 2014. Temperature control module; 2015. Axial fan; 3. Sealing device; 301. Cover plate; 302. Sealing plate; 303. Third mounting bracket; 304. Third limiting rod; 305. Fourth screw rod; 306. Fourth motor; 307. Telescopic rod; 4. Container body; 401. Groove; 402. Culture dish; 403. Working chamber; 404. Illuminating lamp; 405. Bump. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Embodiment 1:

[0032] like Figure 1-7As shown, an embodiment of the present invention provides a cell experiment visualization cell culture device, including an observation device 1, a culture device 2, a sealing device 3 and a plurality of container bodies 4, the culture device 2 is arranged at the top of the observation device 1, the sealing device 3 is arranged at the top of the culture device 2, and the plurality of container bodies 4 are arranged inside the culture device 2, the observation device 1 includes a support plate 101, the top of the support plate 101 is fixedly connected to a support frame 1018 at the rear side, the inner side wall of the support frame 1018 is rotatably connected to a third screw rod 1017, the top of the third screw rod 1017 is fixedly connected to a third motor 1016, and the third The side wall of the screw rod 1017 is threadedly connected with a slider 1015, the front end of the slider 1015 is fixedly connected to the second fixing frame 1014, the bottom end of the second fixing frame 1014 is fixedly connected to the first fixing frame 1013 on the front side, the front end of the second fixing frame 1014 is fixedly connected to the microscope camera 1012, the other end of the first fixing frame 1013 is rotatably connected to the light-transmitting plate 1011, the top of the support plate 101 is fixedly connected to the support plate 101 on the front side, one side of the first mounting frame 102 is fixedly connected to the first motor 103, the inner side wall of the first mounting frame 102 is rotatably connected to the first screw rod 104, and the first mounting frame 102 is fixedly connected to the first motor 103. The front side and the rear side of the inner wall of the mounting frame 102 are respectively fixedly connected with the first limiting rod 105, the side wall of the first screw rod 104 is threadedly connected with the second mounting frame 108, the front end of the second mounting frame 108 is fixedly connected with the second motor 107, the inner wall of the second motor 107 is rotatably connected with the second screw rod 106, the two sides of the inner wall of the second mounting frame 108 are respectively fixedly connected with the second limiting rod 109, the side wall of the second screw rod 106 is threadedly connected with the placement plate 1010, by setting the observation device 1, by rotating the light-transmitting plate 1011 to select different apertures, and at the same time, the first motor 103 drives the second mounting frame 108 to rotate. A screw rod 104 rotates, and the second motor 107 drives the second screw rod 106 to rotate, so that the groove 401 that needs to be observed on the culture dish 402 can be moved to the bottom of the microscope camera 1012. The third motor 1016 drives the third screw rod 1017 to rotate, so that the microscope camera 1012 can find a clearer picture. When shooting, the lighting lamp 404 in the working chamber 403 can be turned on to supplement the light source for shooting, so that the clarity is improved, the part that needs to be observed can be effectively adjusted, the operation difficulty is simplified, and the visualization effect of cell culture is improved.

[0033] The culture device 2 includes a shell 203, a turntable 2010 is rotatably connected to the middle of the inner wall of the shell 203, a temperature sensor 209 is fixedly connected to one side of the inner wall of the shell 203, a humidity sensor 2013 is fixedly connected to the other side of the inner wall of the shell 203, an exhaust pipe 2011 is fixedly connected to the middle of the other side of the shell 203, a second connecting pipe 205 is fixedly connected to the middle of one side of the shell 203, the other end of the first limiting rod 105 is fixedly connected to an air inlet box 206, a temperature control module 2014 is fixedly connected to the bottom end of the inner wall of the air inlet box 206, an air inlet pipe 208 is fixedly connected to one side of the air inlet box 206, a filter screen 207 is respectively provided on the inner wall of the exhaust pipe 2011 and the air inlet pipe 208, an axial flow fan 2015 is fixedly connected to the inner wall of the air inlet pipe 208, and the shell 203 The front side and the rear side are fixedly connected to the insulation shell 204 respectively, the inner wall of the shell 203 is fixedly connected to two connecting pipes 2012, the middle part of the rear end of the insulation shell 204 at the rear end of the shell 203 is fixedly connected to the first connecting pipe 202, the other side of the first connecting pipe 202 is fixedly connected to the constant temperature water tank 201, the bottom end of the shell 203 is fixedly connected to the top of the placement plate 1010, and by setting the culture device 2, during the culture process, the axial flow fan 2015 is used to blow air into the shell 203, the environment in the shell 203 is detected by the temperature sensor 209 and the humidity sensor 2013, and at the same time, the constant temperature circulating water is added to the two insulation shells 204 through the constant temperature water tank 201, so that the culture environment of the cells remains unchanged, which effectively improves the survival rate of the cells during culture.

[0034] The sealing device 3 includes two third mounting brackets 303, the inner side wall of the third mounting bracket 303 at the rear end of the shell 203 is fixedly connected to the third limiting rod 304, the inner side wall of the third mounting bracket 303 at the front end of the shell 203 is rotatably connected to the fourth screw rod 305, one side of the third mounting bracket 303 at the front end of the shell 203 is fixedly connected to the fourth motor 306, the side wall of the fourth screw rod 305 is threadedly connected to the cover plate 301, the top of the cover plate 301 is fixedly connected to the telescopic rod 307, the movable end of the telescopic rod 307 passes through the top of the cover plate 301 and is fixedly connected to the sealing plate 302, and the two The third mounting frame 303 is respectively fixedly connected to the front end and the rear end of the shell 203 at an upper position. By setting the sealing device 3, when it is necessary to observe the cells, the telescopic rod 307 is first retracted to retract the sealing plate 302 from the shell 203, and the fourth motor 306 is turned on. The fourth motor 306 is used to drive the fourth screw rod 305 to rotate, and the sealing plate 302 is moved as a whole to expose the container body 4 as a whole, so as to observe the culture status of the cells, effectively seal the device during the culture process, and avoid the external environment from affecting the container body 4.

[0035] The output end of the first motor 103 passes through the first mounting frame 102 and is fixedly connected to one end of the first screw rod 104. The side walls of the two first limit rods 105 are respectively slidably connected to the bottom end of the second mounting frame 108. The output end of the second motor 107 passes through the front end of the second mounting frame 108 and is fixedly connected to the front end of the second screw rod 106. The side walls of the two second limit rods 109 are respectively slidably connected to the bottom end of the placement plate 1010. The output end of the third motor 1016 passes through the top of the support frame 1018 and is fixedly connected to the top of the third screw rod 1017. The two connecting pipes 2012 respectively pass through the shell The inner wall of the body 203 is connected to the interior of the two insulation shells 204, one end of the first connecting pipe 202 is connected to the interior of the insulation shell 204, one end of the second connecting pipe 205 is connected to the interior of the shell 203, and the other end of the second connecting pipe 205 is connected to the interior of the air intake box 206. The output end of the fourth motor 306 passes through one side of the third mounting frame 303 and is fixedly connected to one end of the fourth screw rod 305. The rear end of the cover plate 301 is slidably connected to the side wall of the third limit rod 304, the sealing plate 302 is slidably connected to the inner wall of the cover plate 301, and the cover plate 301 and the top of the shell 203 are adapted to each other.

[0036] A cell experiment visualization cell culture container comprises a plurality of container bodies 4, wherein the container bodies 4 comprise culture dishes 402, wherein the top of the culture dishes 402 is provided with a plurality of grooves 401, wherein the bottom ends of the inner side walls of the plurality of grooves 401 are respectively provided with a plurality of convex points 405, wherein the bottom ends of the culture dishes 402 are fixedly connected with working chambers 403, wherein the inner side walls of the plurality of working chambers 403 are provided with a plurality of illuminating lamps 404, wherein the bottom ends of the plurality of working chambers 403 are fixedly connected with the top ends of a turntable 2010, wherein the container bodies 4 are provided to add nutrient solution required for cell culture into the plurality of grooves 401, and after placing cell samples, different culture adjustments can be set according to different numbers in the grooves 401 to form a comparison, wherein a control variable experiment can be carried out during the culture process to improve the accuracy of the experimental results, and wherein the illuminating lamps 404 are provided in the working chambers 403 to provide sufficient light sources for observation.

[0037] The bottom of the culture dish 402 is a transparent glass structure, and the bottom ends of the inner side walls of the plurality of grooves 401 are respectively provided with numbers.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell experiment visualization cell culture device, comprising an observation device (1), a culture device (2), a sealing device (3) and a plurality of container bodies (4), wherein the culture device (2) is arranged at the top of the observation device (1), the sealing device (3) is arranged at the top of the culture device (2), and the plurality of container bodies (4) are arranged inside the culture device (2), characterized in that: The observation device (1) comprises a support plate (101), the top end of the support plate (101) is fixedly connected to a support frame (1018) at the rear side, the inner side wall of the support frame (1018) is rotatably connected to a third screw rod (1017), the top end of the third screw rod (1017) is fixedly connected to a third motor (1016), the side wall of the third screw rod (1017) is threadedly connected to a slider (1015), the front end of the slider (1015) is fixedly connected to a second fixed frame (1014), the bottom end of the second fixed frame (1014) is fixedly connected to a first fixed frame (1013) at the front side, the front end of the second fixed frame (1014) is fixedly connected to a microscope camera (1012), the other end of the first fixed frame (1013) is rotatably connected to a light-transmitting plate (1011), and the support plate (101) is fixedly connected to a third motor (1016) at the top end. 01) A support plate (101) is fixedly connected to the front side of the top end, a first motor (103) is fixedly connected to one side of the first mounting frame (102), a first screw rod (104) is rotatably connected to the inner wall of the first mounting frame (102), a first limiting rod (105) is fixedly connected to the front side and the rear side of the inner wall of the first mounting frame (102), a second mounting frame (108) is threadedly connected to the side wall of the first screw rod (104), a second motor (107) is fixedly connected to the front end of the second mounting frame (108), a second screw rod (106) is rotatably connected to the inner wall of the second motor (107), second limiting rods (109) are fixedly connected to both sides of the inner wall of the second mounting frame (108), and a placement plate (1010) is threadedly connected to the side wall of the second screw rod (106).

2. A cell experiment visualization cell culture device according to claim 1, characterized in that: The culture device (2) comprises a shell (203), a turntable (2010) is rotatably connected to the middle of the inner wall of the shell (203), a temperature sensor (209) is fixedly connected to one side of the inner wall of the shell (203), a humidity sensor (2013) is fixedly connected to the other side of the inner wall of the shell (203), an exhaust pipe (2011) is fixedly connected to the middle of the other side of the shell (203), a second connecting pipe (205) is fixedly connected to the middle of one side of the shell (203), the other end of the first limiting rod (105) is fixedly connected to an air inlet box (206), the bottom end of the inner wall of the air inlet box (206) is fixedly connected to a temperature control module (2014), and one side of the air inlet box (206) is fixedly connected to a temperature control module (2014). An air intake pipe (208) is fixedly connected, the exhaust pipe (2011) and the inner side wall of the air intake pipe (208) are respectively provided with a filter screen (207), the inner side wall of the air intake pipe (208) is fixedly connected to an axial flow fan (215), the front side and the rear side of the shell (203) are respectively fixedly connected to an insulation shell (204), the inner side wall of the shell (203) is fixedly connected to two connecting pipes (2012), a first connecting pipe (202) is fixedly connected to the middle of the rear end of the insulation shell (204) at the rear end of the shell (203), the other side of the first connecting pipe (202) is fixedly connected to a constant temperature water tank (201), and the bottom end of the shell (203) is fixedly connected to the top end of the placement plate (1010).

3. A cell culture container and device for visualizing cell experiments according to claim 1, characterized in that: The sealing device (3) comprises two third mounting frames (303), the inner side wall of the third mounting frame (303) located at the rear end of the shell (203) is fixedly connected to a third limiting rod (304), the inner side wall of the third mounting frame (303) located at the front end of the shell (203) is rotatably connected to a fourth screw rod (305), one side of the third mounting frame (303) located at the front end of the shell (203) is fixedly connected to a fourth motor (306), the side wall of the fourth screw rod (305) is threadedly connected to a cover plate (301), the top end of the cover plate (301) is fixedly connected to a telescopic rod (307), the movable end of the telescopic rod (307) passes through the top end of the cover plate (301) and is fixedly connected to a sealing plate (302), and the two third mounting frames (303) are respectively fixedly connected to the upper positions of the front end and the rear end of the shell (203).

4. The cell experiment visualization cell culture device according to claim 1, characterized in that: The output end of the first motor (103) passes through the first mounting frame (102) and is fixedly connected to one end of the first screw rod (104); the two side walls of the first limit rods (105) are respectively slidably connected to the bottom end of the second mounting frame (108); the output end of the second motor (107) passes through the front end of the second mounting frame (108) and is fixedly connected to the front end of the second screw rod (106); the two side walls of the second limit rods (109) are respectively slidably connected to the bottom end of the placement plate (1010); and the output end of the third motor (1016) passes through the top end of the support frame (1018) and is fixedly connected to the top end of the third screw rod (1017).

5. The cell experiment visualization cell culture device according to claim 2, characterized in that: The two connecting pipes (2012) respectively penetrate the inner wall of the shell (203) and are connected to the interior of the two heat-insulating shells (204); one end of the first connecting pipe (202) is connected to the interior of the heat-insulating shell (204); one end of the second connecting pipe (205) is connected to the interior of the shell (203); and the other end of the second connecting pipe (205) is connected to the interior of the air intake box (206).

6. The cell experiment visualization cell culture device according to claim 3, characterized in that: The output end of the fourth motor (306) passes through one side of the third mounting frame (303) and is fixedly connected to one end of the fourth screw rod (305); the rear end of the cover plate (301) is slidably connected to the side wall of the third limit rod (304); the sealing plate (302) is slidably connected to the inner side wall of the cover plate (301); and the cover plate (301) and the top end of the shell (203) are mutually adapted.

7. A cell experiment visualization cell culture container according to claim 1, comprising a plurality of container bodies (4), characterized in that: The container body (4) comprises a culture dish (402), the top of the culture dish (402) is provided with a plurality of grooves (401), the bottom ends of the inner side walls of the plurality of grooves (401) are respectively provided with a plurality of protrusions (405), the bottom end of the culture dish (402) is fixedly connected with a working chamber (403), the inner side walls of the plurality of working chambers (403) are provided with a plurality of lighting lamps (404), and the bottom ends of the plurality of working chambers (403) are fixedly connected with the top of the turntable (2010).

8. A cell experiment visualization cell culture container according to claim 7, characterized in that: The bottom end of the culture dish (402) is a transparent glass structure, and the bottom ends of the inner side walls of the plurality of grooves (401) are respectively provided with numbers.

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