Nucleic acid aptamer specifically binding to arabidopsis cop1 protein and preparation method thereof

Through one-step screening technology and binding site optimization, the nucleic acid aptamers Lib2-11 and Lib2-11c with high affinity and high specificity binding to Arabidopsis COP1 protein were prepared, which solved the time-consuming, complex and costly problems of traditional SELEX screening technology, and realized rapid and low-cost screening and application in synthetic biology.

CN119842718BActive Publication Date: 2025-10-21ANHUI AGRICULTURAL UNIVERSITY
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Patent Information

Application Number
CN202510113870.8
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-01-24
Publication Date
2025-10-21
Estimated Expiration
2045-01-24

AI Technical Summary

Technical Problem

Traditional SELEX screening technology is time-consuming, complex, costly, and not widely applicable. Non-specific binding and PCR amplification deviation are prone to occur during the screening process, which increases the uncertainty of aptamer screening results and makes it difficult to efficiently screen nucleic acid aptamers with high affinity and specific binding to Arabidopsis COP1 protein.

Method used

One-step screening technology was used in combination with Arabidopsis COP1 protein. By predicting the binding site and performing truncation optimization, the nucleic acid aptamer Lib2-11 and its truncated sequence Lib2-11c were prepared. They were analyzed using ELONA and gel retardation experiments to achieve rapid and low-cost specific binding.

Benefits of technology

It achieves high-affinity and high-specificity recognition of Arabidopsis COP1 protein, simplifies the screening process, reduces costs, and improves screening efficiency, making it suitable for the application of synthetic gene circuits and biosensors.

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Abstract

The patent belongs to the field of plant biotechnology, and particularly relates to a nucleic acid aptamer with high binding force specificity to Arabidopsis COP1 protein and a preparation method. To solve the time-consuming and laborious problem of existing screening technology, the patent provides a nucleic acid aptamer with specificity to Arabidopsis COP1 protein and a preparation method. The nucleic acid aptamer Lib2-11 of Arabidopsis COP1 is obtained through One-step screening technology, the nucleic acid aptamer has high binding and high specificity, and after optimization of the sequence, the nucleic acid aptamer Lib2-11c with high binding performance is obtained. The nucleic acid aptamer Lib2-11 and Lib2-11c of the patent have important application prospects for determining the Arabidopsis COP1 protein level, subcellular localization and light-controlled synthetic gene circuit.
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Citation Information

Patent Citations

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  • Combined element for screening aptamer and use thereof, and high-throughput screening method for aptamer

    WO2025010661A1