A Fermentation Temperature Control Method and System
Through the integration of bionic sensors and microwave detection technology, combined with three-stage heating and interlaced magnetic field regulation, the problems of inaccurate temperature control and imbalance of bacterial metabolism in traditional tangerine peel fermentation processes are solved, and the precise control of fermentation temperature and bacterial distribution is achieved, and the uniformity and generation efficiency of the product are improved.
Patent Information
- Application Number
- CN202510354198.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2025-05-30
- Estimated Expiration
- 2045-03-25
AI Technical Summary
Traditional tangerine peel fermentation process is difficult to accurately control the fermentation temperature, resulting in uneven distribution of bacterial metabolic products. Especially in deep fermentation reactors, the mucus layer hinders heat diffusion, resulting in prominent local overheating and regional bacterial metabolic imbalance.
The integrated bionic sensor array and microwave moisture content detection technology are used to obtain the thickening rate of the mucus layer in the fermentation reactor and the distribution of the material's self-weight pressure, and calculate the total amount of biological metabolic heat production based on the dynamic heat production model. Through the three-stage heating program and interlaced magnetic field regulation, the temperature distribution and bacterial flora distribution of the fermentation reactor are adjusted, the wet-guiding film and aroma capture particles are activated, and directional water absorption and heat dissipation are achieved.
Accurate control of fermentation temperature and bacterial flora distribution is achieved, local overheating and bacterial metabolic imbalance are solved, and the uniformity and generation efficiency of secondary metabolites are improved.
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Figure CN119861773B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of tangerine peel fermentation and relates to a fermentation temperature control method and system. Background Art
[0002] In the tangerine peel fermentation process, the dynamic temperature control of the material directly affects the bacterial metabolic pathway and the production efficiency of the target components. Traditional technologies generally rely on manual experience to set a fixed temperature curve, which is difficult to accurately respond to the temperature distribution imbalance caused by the fluctuation of bacterial activity, the sudden change of environmental temperature and humidity, and the heterogeneity of material stratification during the fermentation process. Especially in the deep fermentation pile, the mucus layer formed by the extrusion of the material's own weight hinders the diffusion of heat, resulting in local overheating and regional bacterial metabolic imbalance, which directly affects the uniformity of secondary metabolites.
[0003] Existing technologies build simple feedback systems by installing temperature control devices and conventional sensors. For example, thermocouple temperature control devices are placed in the fermentation box to trigger heating or ventilation based on thresholds. Some optimization methods introduce humidity sensors to adjust the damper opening, or use mechanical stirring to promote heat homogenization. Such solutions rely on linear control logic. Although they can alleviate local overheating, they lack the ability to model the dynamic calculation of heat production by bacterial metabolism, the change in thermal resistance caused by the thickening of the mucus layer, and the nonlinear correlation of multi-parameter coupling to the temperature field.
[0004] Based on the above problems, the disadvantage of the traditional method is that its underlying control logic does not integrate material characteristics and microbial metabolic kinetics models, resulting in data fragmentation and insufficient prediction accuracy; it is unable to adapt to the dynamic heat production laws of the bacterial growth stage in real time, causing excessive heating or ineffective heat dissipation. Summary of the invention
[0005] In order to solve the above problems, the present invention provides a fermentation temperature control method and system.
[0006] In a first aspect, the present invention provides a fermentation temperature control method, which adopts the following technical scheme:
[0007] A fermentation temperature control method comprises the following steps:
[0008] S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material;
[0009] S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages;
[0010] S3. Generate an alternating magnetic field according to the internal temperature gradient distribution of the material, guide the surface flora to migrate to the high magnetic field area in the middle and upper parts, and inhibit the aggregation concentration of the heat-producing flora at the bottom;
[0011] S4. When it is detected that the moisture content in the middle layer exceeds the threshold, activate the directional water absorption program of the moisture-conducting membrane and separate and recover the aromatic substances in the overflow liquid;
[0012] S5. Release aromatic capture particles according to the environmental humidity mutation data, and synchronously open the directional heat dissipation air duct to balance the temperature difference of the material layer;
[0013] S6. Analyze the actual yield of the target component in the finished product, and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
[0014] In a further aspect of the present invention, the step S1 includes the following steps:
[0015] Arrange an anti-pollution sensor with a shark skin bionic structure on the upper layer of the fermented material;
[0016] Obtain the thickness of the mucus layer of the fermented material, and use the microwave resonance principle to detect the moisture content of the material;
[0017] Combine the self-weight pressure data of the fermented material to calculate the total heat production of biological metabolism, and arrange array-type flexible film thermosensitive elements at the bottom of the fermented material.
[0018] In a further aspect of the present invention, the step S2 includes the following steps:
[0019] The first-stage rapid heating stage, raise the temperature to the first critical value at a preset rate, and adjust the over-limit protection threshold of the heating curve according to the real-time heat production;
[0020] The second-stage maintenance stage, dynamically adjust the heating rate in combination with the thickening rate of the mucus layer of the material, and extend the duration of this stage when the heat production fluctuation exceeds the preset range;
[0021] The third-stage convergence stage, judge whether the stability of the heat production increment meets the constant temperature condition, and regulate the metabolic activity ratio of the flora through a multi-frequency pulse cooling mode.
[0022] In a further aspect of the present invention, the step S2 further includes the following steps:
[0023] After the flora enters the mature and stable stage, start the alternating temperature control program. When the monitored value S of the thickness of the middle mucus layer continues to rise, adjust the cooling duration, and satisfy the following formula,
[0024]
[0025] where, represents the duration of the cooling stage, It represents the maximum thickening amount of the middle mucus layer measured within 6 hours. When the microbial community enters the mature and stable stage, a multi-frequency pulse cooling mode is adopted. By alternately changing the high and low temperatures, the ratio of the enzyme activities of the microbial community is changed to promote the accumulation of secondary metabolites.
[0026] In a further aspect of the present invention, step S3 includes the following steps:
[0027] Multiple groups of magnetic field regulating devices are embedded in the side wall of the material box to change the current direction of adjacent coils to generate intersecting magnetic lines penetrating the material; intersecting magnetic lines refer to the area where the magnetic force paths generated by adjacent coils driven by currents in different directions cross and cover each other.
[0028] Based on the real-time magnetic induction intensity distribution data, the surface fermentation microbial community is guided to the high magnetic field area in the upper middle part of the material, and the bottom heat-producing microbial community is gathered in the low magnetic field area at the bottom.
[0029] In a further aspect of the present invention, step S4 includes the following steps:
[0030] When it is monitored that the moisture content in the middle layer exceeds the moisture content threshold, the directional water absorption program of the porous moisture-conducting membrane is started and the separated recovery device is triggered to collect the aromatic substances in the overflow liquid;
[0031] A unidirectional flow path driven by a humidity gradient is established through the moisture-conducting membrane with an asymmetric pore structure;
[0032] The terpene volatile substances in the diverted liquid are separated and recovered by using the condensation temperature difference multi-stage adsorption technology.
[0033] In a further aspect of the present invention, step S5 includes the following steps:
[0034] When the rising speed of the environmental humidity exceeds the threshold within a specified time, the aromatic capture particles wrapped by tangerine peel extract are automatically released; the emergency heat dissipation air duct is started and automatically turns according to the upper, middle and lower layer temperature difference data collected in real time;
[0035] The inner wall of the emergency heat dissipation air duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres, and the surface of this coating has a micron-level protrusion array; the temperature difference data specifically refers to the temperature gradient values of adjacent temperature measurement points in the vertical direction of the fermented material.
[0036] In a further aspect of the present invention, step S6 includes the following steps:
[0037] Through a gas chromatograph analyzer, obtain the content distribution maps of limonene and terpinene in the finished product;
[0038] The deviation rate between the actual yield of the target component and the system prediction value is denoted as K; when the deviation rate K of multiple consecutive batches exceeds the allowable range, a multiple linear regression model is used to update the combined control parameter set of temperature - magnetic field - moisture.
[0039] In a further aspect of the present invention, the deviation rate between the actual yield of the target component and the system prediction value is denoted as K, satisfying the following formula:
[0040]
[0041] where, represents the actual substance content value determined by gas chromatography detection, measured in mass percentage; represents the preset theoretical yield value in the fermentation control system, and the calculated value characterizes the deviation degree between the current batch production process and the ideal model.
[0042] In a second aspect, the present invention provides a fermentation temperature control system, adopting the following technical solution:
[0043] A fermentation temperature control system includes the following modules:
[0044] A data acquisition module, used to obtain the anti - pollution treatment data of the material, detect the moisture content of the middle - layer mucus layer of the material, and calculate the total heat production of biological metabolism by combining the self - weight pressure of the material;
[0045] A temperature regulation module, triggering a hierarchical temperature regulation program according to the total heat production of biological metabolism, and adjusting the temperature distribution of the fermentation heap through the coupled control of three - stage heating and dynamic maintenance stages;
[0046] An alternating magnetic field module, generating an alternating magnetic field according to the internal temperature gradient distribution of the material, guiding the surface - layer bacteria to migrate to the high - magnetic - field area in the middle and upper parts, and inhibiting the aggregation concentration of the bottom - layer heat - producing bacteria;
[0047] A separation and recovery module, used to activate the directional water absorption program of the moisture - conducting membrane and separate and recover the aromatic substances in the overflow liquid when the moisture content of the middle layer is detected to exceed the threshold;
[0048] A directional heat dissipation module, releasing aromatic capture particles according to the sudden change data of environmental humidity, and simultaneously opening the directional heat dissipation air duct to balance the temperature difference of the material layer;
[0049] A process optimization module, used to analyze the actual yield of the target component in the finished product, and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
[0050] In summary, the present invention includes the following beneficial technical effects:
[0051] 1. By integrating a bionic sensor array with microwave moisture detection technology, the thickening rate of the mucus layer in the middle layer of the fermentation heap and the self-weight pressure distribution of the material are obtained in real time. Combining with a dynamic heat generation model, the total amount of heat generated by biological metabolism is calculated. After triggering a three-stage temperature increase program with an over-limit protection mechanism, the heating rate and the duration of each stage are automatically adjusted according to the real-time heat generation fluctuation, and the metabolic activity ratio of the microbial community is regulated through a multi-frequency pulse cooling mode to construct a dynamic temperature gradient that conforms to the growth characteristics of the microbial community, effectively solving the problem of uneven distribution of metabolic products caused by traditional constant-temperature fermentation;
[0052] 2. By using the alternating magnetic fields formed by generating currents in different directions through embedded coils, an intelligent zoning system with high and low magnetic induction intensities is established inside the material. Based on the real-time magnetic induction intensity distribution data, the magnetic field in the middle and upper parts is strengthened, promoting the directional migration of surface microbial communities to the dominant metabolic area, and simultaneously weakening the magnetic field at the bottom layer to inhibit the aggregation of heat-generating microbial communities;
[0053] 3. By obtaining the actual yield of terpene components in the finished product, a mathematical model of the deviation rate from the theoretical predicted value is established, and a multi-variable regression correction factor is generated by correlating the combined control parameters of temperature - magnetic field - moisture. When the deviation rate of consecutive batches exceeds the allowable range, the system iteratively updates the initial control parameters by least squares fitting to form a process self-optimizing closed loop. BRIEF DESCRIPTION OF THE DRAWINGS
[0054] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. The drawings are used to provide a further understanding of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts.
[0055] Figure 1 Disclosed is a flowchart of a fermentation temperature control method.
[0056] Figure 2 Disclosed is a structural diagram of a fermentation temperature control system. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0057] In order to make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.
[0058] The following will make a preferred and detailed description of the present invention with reference to the appended Figure 1 - Figure 2 drawings.
[0059] Refer to the appendedFigure 1 The present invention provides a fermentation temperature control method, comprising the following steps:
[0060] S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material;
[0061] S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages;
[0062] S3, generating a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, and inhibiting the aggregation concentration of the bottom heat-producing bacteria;
[0063] S4, when it is detected that the moisture content of the middle layer exceeds the threshold, the directional water absorption program of the moisture-conducting membrane is activated and the aromatic substances in the overflowed liquid are separated and recovered;
[0064] S5. Release the aroma capture particles according to the sudden change data of the ambient humidity, and simultaneously open the directional heat dissipation duct to balance the temperature difference of the material layer;
[0065] S6. Analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
[0066] In one embodiment of the present invention, step S1 includes the following steps:
[0067] S11, an anti-pollution sensor with a shark skin bionic structure is arranged at a depth of 1 cm above the fermentation material. The sensor uses a 20 Hz high-frequency micro-vibration to vibrate the mycelium attachments to an isolation area with a radius of 5 cm around the sensor;
[0068] Specifically, an anti-pollution sensor with a shark skin bionic structure is deployed at a depth of 1 cm above the fermentation material. The surface of the anti-pollution sensor adopts 3D printing technology to form a gradient groove structure from wide to narrow, and the widest part is gradually reduced from 50 microns to 5 microns. The sensor has an internal integrated piezoelectric vibration module, which generates high-frequency micro-vibrations with an amplitude of 5 microns and a frequency of 20 Hz every 5 seconds. The high-frequency micro-vibration is transmitted to the groove area on the surface of the sensor, and the mycelium and metabolic secretions attached to the surface of the anti-pollution sensor are shaken off to a circular isolation area with a radius of 5 cm. The isolation area is paved with a thin film of hydrophobic material to form a physical barrier to avoid the re-attachment of detached pollutants as much as possible and ensure the stability of the detection signal;
[0069] The anti-pollution sensor is embedded with an array of temperature-sensitive units, with 36 micro-thermocouple probes arranged per square centimeter. When working continuously, the anti-pollution sensor performs a self-cleaning action every 15 minutes to shake off the mycelium clusters attached to the surface.
[0070] Among them, the shark skin bionic structure is a special surface engineering technology that imitates the diamond-shaped scales and grooves on the surface of shark skin. Its microstructure can destroy the balance between the contact area and surface tension required for microbial attachment.
[0071] Piezoelectric vibration module is a transducer device that generates mechanical vibration by applying alternating voltage, and its amplitude is precisely controlled at a microscopic scale to avoid interfering with normal bacterial activity.
[0072] Exemplarily, a conventional sensor and an anti-fouling sensor with a shark skin bionic structure were run in the same fermentation environment for 72 hours;
[0073] The surface of the traditional sensor is covered with a 0.8 mm thick bacterial film, resulting in a temperature detection error of ±2.3°C; the anti-pollution sensor with a shark skin bionic structure has only sporadic attachments on the edge of the sensor, and the maximum detection deviation is controlled within ±0.5°C.
[0074] S12, obtaining the thickness of the mucus layer in the middle layer of the tangerine peel fermentation pile, and when detecting the moisture content of the material using the microwave resonance principle, controlling the transmission power to 0.7 times the ordinary food detection standard to avoid carbonization of the middle mucus layer;
[0075] Specifically, a microwave resonance probe is installed at a depth of 3cm-5cm in the middle layer of the fermentation material. The microwave resonance probe emits a frequency of The water content is calculated by measuring the frequency offset of the reflected wave using the dielectric loss characteristics of the electromagnetic wave in the water-containing medium.
[0076] When the moisture content of materials is detected using the microwave resonance principle, the microwave transmission power is set to 13 milliwatts per square centimeter, which is 0.7 times the standard for conventional food testing. The microwave generator emits electromagnetic waves to the material layer at a frequency of 1 gigahertz, and the energy attenuation signal passing through the material is captured by the antenna array at the receiving end. Targeted power reduction can avoid the formation of hotspot effects in materials containing a middle mucus layer by high-frequency electromagnetic waves, and minimize the carbonization of sugar substances.
[0077] Among them, the microwave resonance principle is the energy absorption phenomenon caused by the interaction between electromagnetic waves of a specific frequency and polar molecules in the substance. The degree of signal attenuation is positively correlated with the water content.
[0078] The hotspot effect refers to the local high temperature phenomenon caused by energy concentration when electromagnetic waves propagate in an inhomogeneous medium.
[0079] S13. Calculate the total amount of heat generated by biological metabolism by combining the self - weight pressure data of the fermentation materials;
[0080] Specifically, a 6×6 array of flexible film thermosensitive elements is arranged at the bottom of the fermentation materials. The size of a single sensing unit is 8 mm×8 mm, and the temperature measurement accuracy is ±0.05°C. A micro - pressure sensor is additionally integrated in the central area of the array, with a pressure - receiving area of 64 square millimeters and a range of 0 - 50 kPa. To calculate the total amount of heat generated by biological metabolism, the following formula is satisfied,
[0081]
[0082] Specifically, represents the total amount of heat generated by biological metabolism, represents the partition temperature value, represents the corresponding azimuth weight factor, represents the material heat conduction correction coefficient, represents the density of the fermentation materials, represents the specific heat capacity.
[0083] Exemplarily, when the temperature at the center of the array is measured to be 42.3°C and the edge temperature is 40.1°C, and the pressure value P = 12 kPa, substituting the preset parameters of tangerine peel fermentation materials ρ = 385 kg / m³, Cp = 1.76 kJ / (kg·°C), the total amount of heat generated by biological metabolism reaches 58.7 W per square meter, triggering the magnetic field regulation device to enhance the bottom - layer heat dissipation efficiency.
[0084] In one embodiment of the present invention, step S2 includes the following steps:
[0085] When the bacteria enter the rapid reproduction stage, a three - level progressive temperature control program is executed;
[0086] In the first - stage rapid heating stage, it lasts for 2 hours, and the initial control target is 42°C. When the actual temperature reaches 40°C, the over - limit protection program is started, and the heating rate is forced to be limited to 1.2°C per hour.
[0087] In the second - stage maintenance stage, it lasts for 4 hours. In this stage, a dynamic heating rate adjustment based on the change of the mycelium mucus layer is adopted, and the heating rate per hour is adjusted to , and at the same time, an index of the temperature balance degree of the inner layer of the material is introduced, and the following formula is satisfied,
[0088]
[0089] Among them, represents the relative change amount of heat production per unit time, represents the average value of heat production in the current detection period, represents the reference value of heat production in the previous detection period; if the increase exceeds per hour When the second level maintenance phase is automatically extended, the maximum allowed extension time is the original setting time. ;
[0090] For example, setting , , calculated ; If the threshold exceeds 5%, the duration of the second stage will be extended from the preset 4 hours to 6 hours.
[0091] In the third convergence stage, it is continuously determined whether the fluctuation amplitude of the heat generation increment is less than 0.8°C for three consecutive sampling cycles. Once this condition is met, the constant temperature holding mode is immediately switched.
[0092] The gradient temperature rise mode during the explosive growth period is a phased and progressive environmental stimulation method. It promotes the rapid formation of dominant colonies by gradually increasing the metabolic load, and at the same time sets a dynamic delay mechanism to prevent overheating damage.
[0093] After the bacterial flora enters the mature and stable stage, the alternating temperature control program is started. The temperature control sequence consists of a 45°C constant temperature section connected to a 38°C pulse cooling section. When the thickness monitoring value S of the middle mucus layer continues to rise, the cooling duration is adjusted to meet the following formula:
[0094]
[0095] in, Indicates the duration of the cooling phase, It indicates the maximum thickening of the middle mucus layer measured within 6 hours. When the bacterial flora enters the mature and stable stage, the multi-frequency pulse cooling mode is used to change the enzyme activity ratio of the bacterial flora through alternating high and low temperatures, thereby promoting the accumulation of secondary metabolites.
[0096] For example, the mucus layer increases from 3.2 mm to 3.7 mm within 6 hours. ; Calculated , select the nearest integer value to perform a cold shock of 8.5 minutes.
[0097] The beneficial effects of this verification example: the physiological characteristics of microorganisms at different growth stages, strengthening the competitiveness of dominant strains through gradient temperature increase, and using pulse cooling to break the metabolic balance and force product synthesis; establishing a parameter adjustment mechanism linked to the metabolic intensity of the flora and the state of by-product accumulation, to avoid the problem of flora degradation that is easily caused by traditional constant temperature cultivation.
[0098] In one embodiment of the present invention, step S3 includes the following steps:
[0099] S31, six sets of magnetic field adjustment devices are embedded in the side wall of the material box, which generate interlaced magnetic lines of force penetrating the material by changing the current direction of adjacent coils;
[0100] Specifically, six groups of magnetic field regulating devices with independent regulation capabilities need to be embedded inside the side wall of the container for the fermented materials. The magnetic field regulating device specifically refers to a multi-turn annular structure formed by winding wires. By inputting currents in different directions to adjacent two groups of coils, it generates a distribution of magnetic force lines that cross each other;
[0101] The current directions of adjacent coils are set as follows: the first group and the second group adopt clockwise winding currents, the third group and the fourth group switch to counterclockwise winding currents, and the fifth group and the sixth group resume clockwise winding currents again. Thus, three alternately arranged magnetic force concentration areas and dispersion areas are formed inside the materials; the intersecting magnetic force lines penetrating the materials refer to the way of crossing and penetrating the magnetic force lines, so that the magnetic induction intensity from the middle layer to the upper part of the fermented materials is maintained in the range of 200 - 300 Gauss, and the magnetic induction intensity in the bottom area of the fermented materials is reduced to the range of 50 - 80 Gauss.
[0102] Among them, the intersecting magnetic force lines refer to the area where the magnetic force paths generated by adjacent coils driven by currents in different directions cross and cover each other.
[0103] Exemplarily, two adjacent groups of coils are selected: the first group of coils is applied with a clockwise current of 15 A, and the second group of coils is applied with a counterclockwise current of 12 A. At this time, the magnetic induction intensity at the center point of the cross magnetic force area formed between the two groups of coils satisfies the following formula,
[0104]
[0105] Among them, represents the magnetic induction intensity at the center point of the cross magnetic force area between the two groups of coils; represents the vacuum permeability; represents the number of turns of the first group of coils; represents the current of the first group of coils; represents the center distance of the first group of coils; represents the number of turns of the second group of coils; represents the current of the second group of coils; represents the center distance of the second group of coils.
[0106] Preset , , , , , ; calculate the magnetic induction intensity at the center point of the cross magnetic force area between the two groups of coils, and verify that under this parameter, the migration rate of the middle layer bacteria group of a single batch of fermented materials to the magnetic force concentration area reaches 82%.
[0107] S32. Guide the surface fermentation flora to the upper-middle high magnetic field area of the material, and make the bottom heat-producing flora gather in the bottom low magnetic field area;
[0108] Specifically, based on the real-time magnetic induction intensity data received by the embedded controller, automatically adjust the current values of each coil to achieve the directional migration of the two types of flora. For the flora on the surface of the fermentation material that needs to improve the metabolic efficiency, guide it to gather in the magnetic force concentration area located in the upper part of the fermentation material. This area generates a co-directional superposition magnetic field through two adjacent groups of coils, forming a stable magnetic field intensity of 280 Gauss in average magnetic induction intensity;
[0109] For the heat-producing flora at the bottom of the fermentation material that needs to inhibit overgrowth, use the magnetic force line divergence area formed by the reverse current between the coil groups to keep the bottom area of the fermentation material in a weak magnetic field environment below 100 Gauss, forcing the flora to maintain its in-situ distribution state.
[0110] Among them, flora guidance specifically refers to the bioelectromagnetic regulation technology that changes the aggregation position of microorganisms by controlling the magnetic field distribution; the magnetic induction intensity superposition principle is the rule of vector addition of the total magnetic field intensity generated by two or more magnetic fields at the same spatial position.
[0111] Exemplarily, when it is detected that the density of the bottom heat-producing flora exceeds the warning value, trigger the intelligent separation program;
[0112] Switch the current directions of the fifth and sixth groups of coils from clockwise to counterclockwise to form a reverse magnetic field superposition with the fourth group of coils; increase the current intensity of the third group of coils from 10 A to 18 A to enhance the magnetic field attraction in the middle and upper layers. The outward diffusion rate of the bottom heat-producing flora decreases by 67% within 72 hours, and the aggregation density of the target flora in the middle and upper layers increases by 51%.
[0113] The beneficial effects of this verification example: effectively inhibit the overgrowth of harmful flora, improve the production efficiency of target metabolites; control the magnetic field space gradient to replace the traditional mechanical separation device, reducing the risk of equipment failure; achieve the active intelligent adjustment of the flora distribution in different fermentation stages, greatly improving the process stability.
[0114] In one embodiment of the present invention, step S4 includes the following steps:
[0115] When the moisture content in the middle layer exceeds the threshold, start the directional water absorption program of the porous moisture-conducting membrane and trigger the separation type recovery device to collect the aromatic substances in the overflow liquid;
[0116] Specifically, when the moisture content value continuously monitored by the microwave resonance probe embedded in the middle-layer material exceeds the set threshold, the directional moisture conduction program of the porous moisture-conducting membrane is automatically activated. The porous moisture-conducting membrane is a composite fiber material with an asymmetric capillary structure, with its hydrophobic side facing the upper layer of the material and its hydrophilic side connected to a hidden water collection tank; when the membrane layer detects that a continuous water film is formed on the contact surface with the material, a transverse micropump is activated to establish a pressure difference gradient, and the excess water is discharged along a preset path to a low-humidity area, avoiding local over-drying caused by traditional dehumidification. For the transferred liquid, the aromatic components contained in it are immediately transported to a multi-stage condensation recovery device, which fixes the volatile substances on the surface of a special adsorbent through temperature difference impact separation technology. The one-way valve group ensures that the liquid flow path is irreversible and prevents the residual moisture from flowing back twice to interfere with the fermentation environment.
[0117] Among them, stratified moisture flow is a phenomenon of directional moisture migration caused by the natural formation of humidity differences at different depths of the fermented material;
[0118] The moisture content threshold represents the upper safety limit of the moisture in the middle layer of the material determined through multiple tests. Exceeding this value will cause disorders in the metabolism of the microbial community; the porous moisture-conducting membrane is a gradient permeable material that imitates the stomatal structure of banyan trees, with special-shaped pores on the surface gradually shrinking from 50 microns to 5 microns, and has the ability of direction-selective moisture conduction;
[0119] The separation recovery device includes three-stage condensation units, which achieve staged capture by utilizing the boiling point difference between aromatic substances and water; the directional water absorption program refers to a programmed control sequence that dynamically adjusts the micropump power and the diversion path according to the real-time humidity distribution of the material; aromatic substances specifically refer to the terpene volatile organic compounds unique to tangerine peel fermentation.
[0120] Exemplarily, in the verification implementation of a certain tangerine peel processing factory, when the microwave resonance probe in the middle layer of the material detects that the moisture content reaches 46% (the critical threshold is 42%), the moisture-conducting membrane automatically activates the 5-minute high-speed diversion mode; the micropump transfers 2.8 L of water to the edge drying area with a negative pressure of 35 kPa. During this period, the pore shrinkage rate of the moisture-conducting membrane automatically matches the humidity gradient; the transferred liquid enters the recovery device, and the cyclodextrin coating captures 91% of the terpinene component.
[0121] The beneficial effects of this verification example: achieving directional dehumidification by identifying abnormal body fluid distribution, getting rid of the high energy consumption defect of traditional equipment; the unique stepped recovery process can, while removing unnecessary moisture, retain the aromatic essence components to the greatest extent.
[0122] In one embodiment of the present invention, step S5 includes the following steps:
[0123] S51, when the ambient humidity rises by more than 30% within 1 hour, the aroma capture particles wrapped by tangerine peel extract are automatically released;
[0124] Specifically, when the ambient humidity rises by more than 30% within 1 hour, the aroma capture particles wrapped in tangerine peel extract are automatically released; when the ambient humidity rises sharply and causes the surface vapor pressure of the fermentation pile to change, the tangerine peel extract protective film on the outer layer of the particles dissolves, exposing the aroma capture particles. The cyclic sugar molecules specifically capture the surrounding environment through host-guest inclusion complexes. Small molecule volatiles such as terpinene and limonene.
[0125] Among them, the aroma capture particles are a composite structure prepared by physical embedding method, and the core has honeycomb holes. cyclic sugar molecular framework; The cyclic sugar molecular framework is a ring-shaped macromolecule formed by multiple glucose units connected end to end. The diameter of the internal cavity is about 0.6-0.8 nanometers, which is just the right size to fit the three-dimensional structure of terpenes in the tangerine peel aroma molecule.
[0126] S52, synchronously start the hidden emergency cooling air duct, and automatically turn according to the real-time collected temperature difference data of the upper, middle and lower layers;
[0127] Specifically, the emergency heat dissipation duct is a spiral guide channel surrounding the side wall of the fermentation box. Its opening position is dynamically adjusted according to the temperature difference data of the upper, middle and lower layers of materials collected in real time. When it is judged that the temperature of the upper layer of materials is higher than that of the middle and lower layers by more than 2°C, the air outlet of the duct automatically turns to the top of the high temperature area, and accelerates local heat dissipation by guiding natural convection in the box. The inner wall of the duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres. The surface of the coating has an array of micron-scale protrusions that can reduce the adhesion of condensed water droplets.
[0128] Among them, the temperature difference data refers specifically to the temperature gradient value of the adjacent temperature measuring points in the vertical direction of the fermentation material, which is obtained by arranging multiple temperature measuring probes at different depths of the fermentation material. The distance between the probes in each layer is not less than 5 cm to avoid thermal field interference.
[0129] Hidden emergency heat dissipation duct refers to a ventilation duct that is completely embedded in the interlayer of the outer wall of the equipment. The diversion outlet of the corresponding section is opened by a mechanical slider only when the emergency control mode is triggered, and it remains closed under normal circumstances.
[0130] Exemplarily, in a certain dried tangerine peel processing factory, a sudden rainstorm caused the environmental humidity in the workshop to rise from 55% to 86% within 45 minutes. The system detected that the humidity change rate exceeded the set threshold and immediately released 300 g of aromatic capture particles from the top of the box. The average particle size of the particles was 80 microns. The sensor monitored an abnormal increase of 0.8 °C in the temperature of the upper layer of the material within 15 minutes, and the emergency air duct automatically adjusted the air outlet direction to the high-temperature area to form local forced convection.
[0131] Beneficial effects of this verification example: The rapid release of aromatic capture particles effectively reduces the risk of aroma component escape, while the intelligent directional heat dissipation air duct avoids the imbalance of microbial activity caused by local overheating; the combination of the two enables the entire control system to quickly inhibit the trend of quality deterioration and maintain the normal progress of microbial metabolic activities when facing sudden working conditions, significantly improving the anti-interference ability of the production process and the product quality consistency; in the actual production environment, even in the face of extreme temperature and humidity fluctuations, the aroma characteristic substances of the fermentation products are still ensured to be completely retained.
[0132] In one embodiment of the present invention, step S6 includes the following steps:
[0133] Obtain the content distribution map of limonene and terpinene in the finished product through a gas chromatograph analyzer;
[0134] Specifically, the dried tangerine peel fermentation sample after drying is crushed and placed at the injection port. The mobile carrier gas carries the volatiles through a capillary column coated with a stationary liquid. Different components are separated due to the difference in adsorption force, and the peak emergence time and area of target substances such as limonene and terpinene are captured by a flame ionization detector to generate a two-dimensional waveform map. The height of the chromatographic peak corresponds to the substance concentration, and the width at the base of the peak reflects the separation purity.
[0135] Among them, the gas chromatograph analyzer is a precision detection instrument based on the difference in the volatility of substances, and its core includes a gas separation column and a signal detector.
[0136] Denote the deviation rate between the actual yield of the target component and the system prediction value as K, and generate an optimization coefficient according to the formula, satisfying the following formula,
[0137]
[0138] Among them, represents the actual substance content value determined by gas chromatographic detection, measured in mass percentage; represents the preset theoretical yield value in the fermentation control system, and the calculated value characterizes the deviation degree between the current batch production process and the ideal model.
[0139] When the absolute value of Automatically trigger the parameter self-learning module to update the initial control parameter combination for the next batch;
[0140] Specifically, the parameter self-learning module integrates an intelligent adjustment unit for the multiple linear regression algorithm, which receives the deviation rate , the duration of the temperature control stage, and the magnetic field action intensity, and fits the correction law in the historical dataset through the least squares method; the updated parameter combination includes control variables such as the magnetic field action time weighting coefficient and the upper limit of the single-step temperature increase amplitude;
[0141] Exemplarily, when detecting that the terpene K value of 3 consecutive batches reaches , the parameter self-learning module will increase the middle-layer magnetic field intensity by , and reduce the temperature climbing rate by . Subsequently, the K value of the next two batches is called back to .
[0142] See the appendix Figure 2 , the present invention also proposes a fermentation temperature control system, including the following modules:
[0143] A data acquisition module, used to obtain the anti-pollution treatment data of the material, detect the moisture content of the middle-layer mucus layer of the material, and calculate the total heat generated by biological metabolism in combination with the self-weight pressure of the material;
[0144] A temperature control module, which triggers a hierarchical temperature control program according to the total heat generated by biological metabolism, and adjusts the temperature distribution of the fermentation pile through the coupled control of the three-stage temperature increase and the dynamic maintenance stage;
[0145] An alternating magnetic field module, which generates an alternating magnetic field according to the internal temperature gradient distribution of the material, guides the surface flora to migrate to the high magnetic field area in the middle and upper parts, and inhibits the aggregation concentration of the bottom heat-producing flora;
[0146] A separation and recovery module, which activates the directional water absorption program of the moisture-conducting membrane and separates and recovers the aromatic substances in the overflow liquid when detecting that the middle-layer moisture content exceeds the threshold;
[0147] A directional heat dissipation module, which releases aromatic capture particles according to the sudden change data of the environmental humidity, and simultaneously opens the directional heat dissipation air duct to balance the temperature difference of the material layer;
[0148] A process optimization module, which is used to analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
[0149] Each of the above-mentioned modules can be implemented in whole or in part by software, hardware, and their combinations, supports being embedded in the processor of the computer device in hardware form or being independent of it, and also supports being stored in the memory of the computer device in software form, so as to facilitate the processor to call and execute the operations corresponding to each of the above modules.
[0150] It should be noted that the human body information (including but not limited to human body device information and personal information, etc.) and data (including but not limited to data for analysis, stored data, displayed data, etc.) involved in the present invention are all information and data authorized by the human body or fully authorized by all parties. The collection, use, and processing of relevant data require relevant legal standards.
[0151] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the various embodiments of the present invention, and should all be included in the protection scope of the present invention.
Claims
1. A fermentation temperature control method, characterized in that: The following steps are involved: S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material; S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; S3, generating a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, and inhibiting the aggregation concentration of the bottom heat-producing bacteria; S4, when it is detected that the moisture content of the middle layer exceeds the threshold, the directional water absorption program of the moisture-conducting membrane is activated and the aromatic substances in the overflowed liquid are separated and recovered; S5. Release the aroma capture particles according to the sudden change data of the ambient humidity, and simultaneously open the directional heat dissipation duct to balance the temperature difference of the material layer; S6. Analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
2. A fermentation temperature control method according to claim 1, characterized in that: The step S1 comprises the following steps: Anti-pollution sensors with shark skin bionic structures are arranged on the upper layer of the fermentation material; Obtain the thickness of the mucus layer of the fermented material and use the microwave resonance principle to detect the moisture content of the material; The total amount of heat generated by biological metabolism is calculated in combination with the deadweight pressure data of the fermentation materials, and an array of flexible thin film thermistors is arranged at the bottom of the fermentation materials.
3. A fermentation temperature control method according to claim 1, characterized in that: The step S2 comprises the following steps: In the first stage of rapid heating, the temperature is raised to the first critical value at a preset rate, and the over-limit protection threshold of the heating curve is adjusted according to the real-time heat generation; In the second maintenance stage, the heating rate is dynamically adjusted in combination with the thickening rate of the mucus layer of the material, and the duration of this stage is extended when the heat generation fluctuation exceeds the preset range; In the third convergence stage, it is determined whether the stability of the heat production increment meets the constant temperature conditions, and the metabolic activity ratio of the bacterial community is regulated by a multi-frequency pulse cooling mode.
4. A fermentation temperature control method according to claim 3, characterized in that: The step S2 further comprises the following steps: After the bacterial flora enters the mature and stable stage, the alternating temperature control program is started. When the thickness monitoring value S of the middle mucus layer continues to rise, the cooling duration is adjusted to meet the following formula: in, Indicates the duration of the cooling phase, It indicates the maximum thickening of the middle mucus layer measured within 6 hours. The bacterial flora enters a mature and stable stage. The multi-frequency pulse cooling mode is used to change the enzyme activity ratio of the bacterial flora through alternating high and low temperatures to promote the accumulation of secondary metabolites.
5. A fermentation temperature control method according to claim 4, characterized in that: The step S3 comprises the following steps: Multiple sets of magnetic field adjustment devices are embedded in the side wall of the material box to change the current direction of adjacent coils to generate staggered magnetic lines of force that penetrate the material; staggered magnetic lines of force are areas where the magnetic paths generated by adjacent coils driven by currents in different directions intersect and cover each other; Based on the real-time magnetic induction intensity distribution data, the surface fermentation bacteria are guided to the high magnetic field area in the upper middle part of the material, and the bottom heat-producing bacteria are gathered in the low magnetic field area at the bottom.
6. A fermentation temperature control method according to claim 5, characterized in that: The step S4 comprises the following steps: When the moisture content of the monitored middle layer exceeds the moisture content threshold, the directional water absorption program of the porous moisture-conducting membrane is started and the separation recovery device is triggered to collect the aromatic substances in the overflowed liquid; A unidirectional flow path driven by humidity gradient is established through a moisture-conducting membrane with an asymmetric pore structure; The terpene volatile substances in the diversion liquid are separated and recovered by using the condensation temperature difference multi-stage adsorption technology.
7. A fermentation temperature control method according to claim 6, characterized in that: The step S5 comprises the following steps: When the rate of increase of ambient humidity exceeds the threshold within the specified time, the aroma capture particles wrapped in tangerine peel extract are automatically released; the emergency heat dissipation duct is activated, and the air flow direction is automatically changed according to the real-time collected temperature difference data of the upper, middle and lower layers; The inner wall of the emergency heat dissipation duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres, and the surface of the coating has an array of micron-scale protrusions; the temperature difference data specifically refers to the temperature gradient value of adjacent temperature measuring points in the vertical direction of the fermentation material.
8. A fermentation temperature control method according to claim 7, characterized in that: The step S6 comprises the following steps: The limonene, Terpinene content distribution map; The deviation rate between the actual yield of the target component and the system predicted value is recorded as K; when the deviation rate K of multiple consecutive batches exceeds the allowable range, the multivariate linear regression model is used to update the joint control parameter combination of temperature, magnetic field and moisture.
9. A fermentation temperature control method according to claim 8, characterized in that: The deviation rate between the actual yield of the target component and the system predicted value is recorded as K, which satisfies the following formula: in, Indicates the actual substance content value determined by gas chromatography, measured in mass percentage; Indicates the theoretical yield value preset in the fermentation control system. The value represents the degree of deviation between the current batch production process and the ideal model.
10. A fermentation temperature control system, characterized in that: Includes the following modules: The data acquisition module is used to obtain the anti-fouling treatment data of the material, detect the moisture content of the mucus layer in the material, and calculate the total heat generated by biological metabolism based on the material's deadweight pressure; The temperature control module triggers the graded temperature control program according to the total amount of heat produced by biological metabolism, and adjusts the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; The staggered magnetic field module generates a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, inhibiting the aggregation concentration of the bottom heat-producing bacteria; A separation and recovery module is used to detect that the moisture content of the middle layer exceeds a threshold value, activate the directional water absorption program of the moisture-conducting membrane and separate and recover the aromatic substances in the overflowed liquid; Directional heat dissipation module releases aroma capture particles according to the sudden change data of ambient humidity, and simultaneously opens the directional heat dissipation duct to balance the temperature difference of the material layer; The process optimization module is used to analyze the actual yield of the target component in the finished product and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
Citation Information
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