Sterile culture equipment for biological tissue culture body

Abstract

The invention discloses sterile culture equipment for a biological tissue culture body, which relates to the technical field of biological tissue culture, and adopts the technical scheme that the sterile culture equipment comprises an operation table, a disinfection tank is fixedly connected to the upper part of the operation table, a sterile water tank is arranged at the upper part of the disinfection tank, and the sterile water tank is fixedly connected with the operation table; a sterile water tank is arranged in the sterile water tank, a disinfection tank is arranged in the sterile water tank, an alcohol tank and a mercury bichloride solution tank are arranged on the right side and the rear side of the disinfection tank, the sterile water tank, the alcohol tank and the mercury bichloride solution tank are all provided with conveying pipes, and a rotating shell is fixedly connected to the interior of the disinfection tank. Then a movable baffle is controlled to rotate through a third motor in a control shell, a notch formed in the upper portion of the control shell is opened, then a sterile water tank is controlled to spray sterile water to clean the surface of the explant, and in the sterile water spraying process, a fourth motor in a rotating shell can be controlled to drive a containing filter disc to rotate; therefore, the cleaning effect of the explant is improved.

Description

Technical Field

[0001] The invention relates to the technical field of biological tissue culture, in particular to a sterile culture device for a biological tissue culture body. Background Art

[0002] Sterile culture equipment for biological tissue cultures is an important tool in the field of biotechnology. Its background technology comes from long-term research and exploration of sterile operating environments. These devices use a series of technical means, such as the use of high-pressure sterilizers, ultraviolet sterilization lamps, etc., to ensure the sterility of experimental equipment and reagents, and provide a stable and sterile growth environment for cell or tissue culture. The development of sterile culture equipment has undergone years of technological accumulation and innovation, and has now become an indispensable technical support for cell culture, tissue engineering, plant breeding and other fields. With the rapid development of biotechnology, sterile culture equipment is also constantly being updated and replaced, developing in a more efficient, intelligent and environmentally friendly direction, providing strong support for biological science research and production practice.

[0003] After searching, the Chinese patent number CN217265797U discloses a sterile operating table for cell culture, wherein a support table is arranged at the upper end of the grounding platform, a culture table top is arranged at the upper end of the support table, a cell culture medium is arranged at the upper end of the culture table top, ultraviolet sterilization lamps are arranged on both sides of the cell culture medium, a sealed outer cover is arranged outside the two ultraviolet sterilization lamps, an outer cover port is arranged at the front end position of the sealed outer cover, a door panel closing frame is arranged at both ends of the outer cover port, a sealed door panel is installed between the two door panel closing frames, ventilation ports are arranged on the outer walls of both sides of the sealed outer cover, a movable flap is arranged inside the ventilation port, and a distribution box is arranged on the outer wall of one side of the support table. Compared with the prior art, the Chinese patent number CN217265797U solves the problem that most of the existing cell culture operating tables are exposed to the outside and cannot effectively provide a sterile culture environment for cells at the operation site.

[0004] However, in actual use, the above-mentioned device is crucial for the aseptic disinfection of explants when culturing plant tissues. However, the existing disinfection is mostly carried out by manual spraying of sterile water, which not only has poor disinfection effect but also low disinfection efficiency. Therefore, a sterile culture device for biological tissue culture is proposed. Summary of the invention

[0005] The purpose of the present invention is to solve the problem in the prior art that sterilization of explants is crucial when culturing plant tissues, but the existing disinfection is mostly carried out by manual spraying of sterile water, which not only has poor disinfection effect but also low disinfection efficiency, and proposes a sterile culture device for biological tissue culture bodies.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A sterile culture device for a biological tissue culture body, comprising an operating table, a disinfection tank is fixedly connected to the upper part of the operating table, a sterile water tank is arranged on the upper part of the disinfection tank, the sterile water tank is fixedly connected to the operating table, an alcohol tank and a mercuric chloride solution tank are arranged on the right and rear sides of the disinfection tank, the sterile water tank, the alcohol tank and the mercuric chloride solution tank are all equipped with a delivery pipe, a rotating shell is fixedly connected inside the disinfection tank, a fourth motor is arranged inside the rotating shell, a third gear is arranged at the output end of the fourth motor, the third gear is meshedly connected with a fourth gear, the fourth gear is rotationally connected to the rotating shell, and a filter disc is fixedly connected to the side of the fourth gear away from the rotating shell;

[0008] A control shell is fixedly connected to the lower part of the disinfection tank, a slot is provided on the upper part of the control shell, a third motor is arranged inside the control shell, a first gear is arranged on the output end of the third motor, the first gear is meshingly connected to the second gear, five eccentric shafts are rotatably connected to the upper part of the second gear, a movable baffle is rotatably connected to the upper part of the eccentric shaft, the movable baffle is rotatably connected to the control shell, and the five movable baffles are driven to rotate by the third motor, thereby controlling the opening and closing of the slot on the upper part of the control shell, and by closing the slot, the alcohol tank and the mercuric chloride solution tank transport liquid into the disinfection tank to immerse and sterilize the explant placed in the filter disc, and the control slot is opened to rinse the residual liquid on the explant with the sterile water in the sterile water tank, and a water pump is arranged inside the infusion tube, and the liquid in the sterile water tank, the alcohol tank and the mercuric chloride solution tank can be sprayed out through the delivery tube by the water pump, and a plurality of drainage ports are arranged at the bottom of the filter disc.

[0009] The above technical solution further includes:

[0010] A drain pipe is fixedly connected inside the control housing, the upper end of the drain pipe is in contact with the movable baffle, and the lower end of the drain pipe is inserted into the waste box.

[0011] Two transmission mechanisms are symmetrically fixedly connected to the front end of the operating table, and the transmission mechanisms drive the sterile baffle arranged on the upper part to move up and down. A storage groove is opened on the top of the operating table for the sterile baffle to move up and enter.

[0012] The transmission mechanism comprises a transmission housing fixedly connected to the front end of the operating table, a first motor is arranged on the upper part of the transmission housing, and a transmission assembly is arranged at the output end of the first motor.

[0013] The transmission assembly includes a second screw rod arranged at the output end of the first motor, the second screw rod is rotatably connected to the transmission housing, the second screw rod is threadedly connected to a transmission member, the transmission member is slidably connected to the transmission housing, and a sterile baffle is fixedly connected to the side of the transmission member away from the transmission housing.

[0014] An adjusting mechanism is arranged on the upper part of the operating table, and an ultraviolet lamp is fixedly connected to the lower part of the adjusting mechanism, so that the ultraviolet lamp is driven to move by the adjusting mechanism.

[0015] The adjustment mechanism comprises an adjustment slot fixedly connected to the upper part of the operating table, a second motor is arranged on the right side of the adjustment slot, and an adjustment component is arranged at the output end of the second motor.

[0016] The adjustment component includes a first screw rod arranged at the output end of the second motor, the first screw rod is rotatably connected to the adjustment slot, the first screw rod is threadedly connected to an adjustment plate, and the lower part of the adjustment plate is fixedly connected to an ultraviolet lamp.

[0017] Four limit sliders are fixedly connected to the upper part of the adjustment plate, and the four limit sliders are slidably connected to the adjustment slot. Two slide rails are slidably connected to the limit sliders at the lower part of the adjustment slot.

[0018] The present invention has the following beneficial effects:

[0019] 1. In the present invention, when the device is used, personnel can put the selected explant into the placement filter plate inside the disinfection tank, and then control the movable baffle to rotate by the third motor inside the control shell, open the slot set on the upper part of the control shell, and then control the sterile water tank to spray sterile water to clean the surface of the explant. In the process of spraying sterile water, the fourth motor inside the rotating shell can be controlled to drive the placement filter plate to rotate, thereby improving the cleaning effect of the explant. After the cleaning is completed, the slot is closed by controlling the liquid in the alcohol tank and the mercuric chloride solution tank to be sent into the disinfection tank one by one to soak the explant. After the soaking is completed, it is rinsed with sterile water. Through multiple sterilization and disinfection operations, the explant can be effectively sterilized and disinfected, and the effect of subsequent cultivation is effectively improved. Moreover, the entire sterilization and disinfection process does not require manual operation by personnel, which is convenient and efficient.

[0020] 2. In the present invention, the two transmission mechanisms arranged at the front end of the operating table can drive the sterile baffle to move up and down. When a person extends his hand from the lower end of the sterile baffle into the interior of the operating table, the movement of the sterile baffle can be controlled to provide the person's hands with sufficient space for movement while ensuring that the sterile baffle has sufficient shielding area, thereby effectively ensuring the flexibility of the person's hands. During cultivation, the ultraviolet lamp can also be driven to move through the adjustment mechanism. By controlling the ultraviolet lamp to follow the movement of the explant throughout the process, it can be effectively ensured that the cultivation process is always within the effective irradiation range of the ultraviolet lamp. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 A schematic diagram of the structure of a sterile culture device for a biological tissue culture body proposed by the present invention;

[0022] Figure 2 It is a schematic diagram of the internal structure of the operating table in the present invention;

[0023] Figure 3 It is a schematic diagram of the internal structure of the transmission housing in the present invention;

[0024] Figure 4 A bottom view of the adjustment mechanism of the present invention;

[0025] Figure 5 It is a schematic diagram of the internal structure of the adjustment tank in the present invention;

[0026] Figure 6 It is a schematic diagram of the connection relationship of the disinfection tank in the present invention;

[0027] Figure 7 It is a schematic diagram of the internal structure of the disinfection tank in the present invention;

[0028] Figure 8 It is a schematic diagram of the internal structure of the control housing in the present invention;

[0029] Fig. 9 It is a schematic diagram of the internal structure of the rotating shell in the present invention.

[0030] In the figure: 1. operating table; 2. waste box; 3. transmission housing; 4. sterile baffle; 5. sterile water tank; 6. alcohol tank; 7. mercuric chloride solution tank; 8. disinfection tank; 9. ultraviolet lamp; 10. adjustment tank; 11. first screw rod; 12. first motor; 13. second screw rod; 14. transmission member; 15. second motor; 16. adjustment plate; 17. limit slider; 18. filter plate; 19. drain pipe; 20. control housing; 21. rotating housing; 22. third motor; 23. first gear; 24. second gear; 25. eccentric shaft; 26. movable baffle; 27. fourth motor; 28. third gear; 29. ​​fourth gear. DETAILED DESCRIPTION

[0031] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Embodiment 1

[0033] like Figure 1-Figure 9 As shown, a sterile culture device for biological tissue culture bodies includes an operating table 1, a disinfection tank 8 is fixedly connected to the upper part of the operating table 1, a sterile water tank 5 is arranged on the upper part of the disinfection tank 8, the sterile water tank 5 is fixedly connected to the operating table 1, an alcohol tank 6 and a mercuric chloride solution tank 7 are arranged on the right and rear sides of the disinfection tank 8, the sterile water tank 5, the alcohol tank 6 and the mercuric chloride solution tank 7 are all equipped with conveying pipes, a rotating shell 21 is fixedly connected inside the disinfection tank 8, a fourth motor 27 is arranged inside the rotating shell 21, a third gear 28 is arranged at the output end of the fourth motor 27, the third gear 28 is meshedly connected with a fourth gear 29, the fourth gear 29 is rotationally connected to the rotating shell 21, and a filter plate 18 is fixedly connected to the side of the fourth gear 29 away from the rotating shell 21.

[0034] The lower part of the disinfection tank 8 is fixedly connected to a control housing 20, and a notch is opened on the upper part of the control housing 20. A third motor 22 is arranged inside the control housing 20, and a first gear 23 is arranged at the output end of the third motor 22. The first gear 23 is meshed and connected with a second gear 24. Five eccentric shafts 25 are rotatably connected to the upper part of the second gear 24, and a movable baffle 26 is rotatably connected to the upper part of the eccentric shaft 25. The movable baffle 26 is rotatably connected to the control housing 20, and the five movable baffles 26 are driven by the third motor 22 to emit The control housing 20 rotates to control the opening and closing of the slot on the upper portion of the control housing 20. By closing the slot, the alcohol tank 6 and the mercuric chloride solution tank 7 transport liquid to the disinfection tank 8 to immerse and sterilize the explant placed in the filter disc 18. The slot is controlled to open, and the residual liquid on the explant is rinsed with sterile water in the sterile water tank 5. A water pump is provided inside the infusion tube, and the liquid in the sterile water tank 5, the alcohol tank 6 and the mercuric chloride solution tank 7 can be sprayed out through the delivery tube by the water pump. A plurality of drainage ports are provided at the bottom of the filter disc 18.

[0035] A drain pipe 19 is fixedly connected to the inside of the control housing 20 , the upper end of the drain pipe 19 is in contact with the movable baffle 26 , and the lower end of the drain pipe 19 is inserted into the waste box 2 .

[0036] In this embodiment, when the device is used, a person can place the selected explant into the filter plate 18 inside the disinfection tank 8, and then start the third motor 22, and the first gear 23 is driven to rotate by the third motor 22, and the first gear 23 is driven to rotate by the second gear 24, and the second gear 24 is driven to rotate by the five eccentric shafts 25 connected in rotation, and the movable baffle 26 connected in rotation is also driven to rotate by the rotation of the eccentric shaft 25, and the rotation of the movable baffle 26 can control the opening and closing of the upper notch of the control housing 20. After the notch set on the upper part of the control housing 20 is opened, the sterile water in the sterile water tank 5 is controlled to spray out to clean the surface of the explant;

[0037] During the process of spraying sterile water, the fourth motor 27 is started to drive the third gear 28 to rotate, and the rotation of the third gear 28 drives the fourth gear 29 to rotate. The rotation of the fourth gear 29 can drive the upper fixed filter plate 18 to rotate, and then drive the explant placed inside the filter plate 18 to rotate, thereby effectively improving the cleaning effect of sterile water. After the cleaning is completed, the slot is controlled to close, and then the alcohol tank 6 is controlled to transport alcohol into the disinfection tank 8. After the explant is soaked in the alcohol for a few seconds, the slot is opened and then rinsed with sterile water. After the rinsing is completed, the internal solution is transported through the mercuric chloride solution tank 7. After soaking for a few minutes, the explant is thoroughly rinsed with sterile water to remove residual disinfectant. Through multiple sterilization and disinfection operations, the explant can be effectively sterilized and disinfected, which effectively improves the effect of subsequent cultivation. Moreover, the entire sterilization and disinfection process does not require manual operation by personnel, which is convenient and efficient.

[0038] Embodiment 2

[0039] like Figure 1-Figure 9 As shown, two transmission mechanisms are symmetrically fixedly connected to the front end of the operating table 1, and the transmission mechanism drives the sterile baffle 4 arranged on the upper part to move up and down. A storage groove is opened on the top of the operating table 1 for the sterile baffle 4 to move up and enter. The transmission mechanism includes a transmission housing 3 fixedly connected to the front end of the operating table 1, a first motor 12 is arranged on the upper part of the transmission housing 3, and a transmission assembly is arranged at the output end of the first motor 12. The transmission assembly includes a second screw rod 13 arranged at the output end of the first motor 12, the second screw rod 13 is rotatably connected to the transmission housing 3, the second screw rod 13 is threadedly connected to a transmission member 14, the transmission member 14 is slidably connected to the transmission housing 3, and the sterile baffle 4 is fixedly connected to the side of the transmission member 14 away from the transmission housing 3.

[0040] An adjusting mechanism is provided on the upper part of the operating table 1, and an ultraviolet lamp 9 is fixedly connected to the lower part of the adjusting mechanism. The ultraviolet lamp 9 is driven to move by the adjusting mechanism. The adjusting mechanism includes an adjusting slot 10 fixedly connected to the upper part of the operating table 1, and a second motor 15 is provided on the right side of the adjusting slot 10. An adjusting component is provided at the output end of the second motor 15. The adjusting component includes a first screw rod 11 provided at the output end of the second motor 15, and the first screw rod 11 is rotatably connected to the adjusting slot 10. An adjusting plate 16 is threadedly connected to the first screw rod 11, and the ultraviolet lamp 9 is fixedly connected to the lower part of the adjusting plate 16. Four limit sliders 17 are fixedly connected to the upper part of the adjusting plate 16, and the four limit sliders 17 are slidably connected to the adjusting slot 10. Two slide rails are provided at the lower part of the adjusting slot 10 and are slidably connected to the limit slider 17.

[0041] In this embodiment, the second screw rod 13 is driven to rotate by starting the first motor 12, and the rotation of the second screw rod 13 can drive the threaded transmission member 14 to move up and down, and the up and down movement of the transmission member 14 can drive the fixedly connected sterile baffle 4 to move up and down. When a person extends his hand from the lower end of the sterile baffle 4 into the interior of the operating table 1, the movement of the sterile baffle 4 can be controlled to provide the person with sufficient movement space for both hands while ensuring that the sterile baffle 4 has sufficient shielding area, thereby effectively ensuring the flexibility of the person's hands. During cultivation, the first screw rod 11 is driven to rotate by the second motor 15, and the rotation of the first screw rod 11 can drive the threaded adjustment plate 16 to move, and during the movement of the adjustment plate 16, the upper fixedly connected limit slider 17 can synchronously slide along the adjustment slot 10, effectively ensuring the stability of the movement of the adjustment plate 16, and the movement of the adjustment plate 16 can drive the ultraviolet lamp 9 to move, and the ultraviolet lamp 9 is controlled to follow the movement of the explant throughout the process, which can effectively ensure that the cultivation process is always within the effective irradiation range of the ultraviolet lamp 9.

[0042] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sterile culture device for biological tissue culture, comprising an operating table (1), characterized in that: A disinfection tank (8) is fixedly connected to the upper part of the operating table (1), a sterile water tank (5) is arranged on the upper part of the disinfection tank (8), the sterile water tank (5) and the operating table (1) are fixedly connected, an alcohol tank (6) and a mercuric chloride solution tank (7) are arranged on the right side and the rear side of the disinfection tank (8), the sterile water tank (5), the alcohol tank (6) and the mercuric chloride solution tank (7) are all equipped with conveying pipes, a rotating shell (21) is fixedly connected inside the disinfection tank (8), a fourth motor (27) is arranged inside the rotating shell (21), a third gear (28) is arranged at the output end of the fourth motor (27), the third gear (28) is meshedly connected with a fourth gear (29), the fourth gear (29) is rotationally connected to the rotating shell (21), and a filter disc (18) is fixedly connected to the side of the fourth gear (29) away from the rotating shell (21); The lower part of the disinfection tank (8) is fixedly connected to a control housing (20), the upper part of the control housing (20) is provided with a notch, a third motor (22) is arranged inside the control housing (20), the output end of the third motor (22) is provided with a first gear (23), the first gear (23) is meshedly connected to a second gear (24), the upper part of the second gear (24) is rotatably connected to five eccentric shafts (25), the upper part of the eccentric shaft (25) is rotatably connected to a movable baffle (26) ), the movable baffle (26) is rotatably connected to the control housing (20), and the five movable baffles (26) are driven to rotate by the third motor (22), thereby controlling the opening and closing of the upper notch of the control housing (20). By closing the notch, the alcohol tank (6) and the mercuric chloride solution tank (7) transport liquid into the disinfection tank (8) to immerse and sterilize the explant placed in the filter plate (18). The control notch is opened, and the residual liquid on the explant is washed by the sterile water in the sterile water tank (5).

2. The aseptic culture device for biological tissue culture according to claim 1, characterized in that: A drain pipe (19) is fixedly connected to the inside of the control housing (20), the upper end of the drain pipe (19) is in contact with the movable baffle (26), and the lower end of the drain pipe (19) is inserted into the inside of the waste box (2).

3. The aseptic culture device for biological tissue culture according to claim 1, characterized in that: The front end of the operating table (1) is symmetrically fixedly connected to two transmission mechanisms, and the transmission mechanisms drive a sterile baffle (4) arranged on the upper part to move up and down.

4. The aseptic culture device for biological tissue culture according to claim 3, characterized in that: The transmission mechanism comprises a transmission housing (3) fixedly connected to the front end of the operating table (1); a first motor (12) is arranged on the upper part of the transmission housing (3); and a transmission assembly is arranged at the output end of the first motor (12).

5. The aseptic culture device for biological tissue culture according to claim 4, characterized in that: The transmission assembly comprises a second screw rod (13) arranged at the output end of the first motor (12), the second screw rod (13) being rotationally connected to the transmission housing (3), the second screw rod (13) being threadedly connected to a transmission member (14), the transmission member (14) being slidingly connected to the transmission housing (3), and a sterile baffle (4) being fixedly connected to a side of the transmission member (14) away from the transmission housing (3).

6. The aseptic culture device for biological tissue culture according to claim 1, characterized in that: An adjustment mechanism is provided on the upper part of the operating table (1), and an ultraviolet lamp (9) is fixedly connected to the lower part of the adjustment mechanism, so that the ultraviolet lamp (9) is driven to move by the adjustment mechanism.

7. The aseptic culture device for biological tissue culture according to claim 6, characterized in that: The adjustment mechanism comprises an adjustment slot (10) fixedly connected to the upper part of the operating table (1), a second motor (15) is arranged on the right side of the adjustment slot (10), and an adjustment component is arranged at the output end of the second motor (15).

8. The aseptic culture device for biological tissue culture according to claim 7, characterized in that: The adjustment assembly comprises a first screw rod (11) arranged at the output end of a second motor (15), the first screw rod (11) being rotatably connected to an adjustment slot (10), the first screw rod (11) being threadedly connected to an adjustment plate (16), and an ultraviolet lamp (9) being fixedly connected to the lower part of the adjustment plate (16).

9. The aseptic culture device for biological tissue culture according to claim 8, characterized in that: Four limiting slide blocks (17) are fixedly connected to the upper portion of the adjustment plate (16), and the four limiting slide blocks (17) are slidably connected to the adjustment slots (10).

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