Nano-functionalized antibacterial deoiling washing-free cleaning towel as well as preparation method and application thereof

Abstract

The invention provides a nano-functionalized antibacterial deoiling washing-free cleaning towel. The cleaning towel is prepared from a carrier and the antibacterial liquid. The invention provides the nano-functionalized antibacterial and deoiling washing-free cleaning towel with cleaning and bacteriostasis functions, so that the personalized and quick cleaning requirements of personnel in special scenes are met. The problems of component irritation, poor use feeling, weak detergency, narrow antibacterial range and the like of an existing washing-free cleaning towel are solved, and the cleaning towel is convenient to use, non-toxic, environmentally friendly and high in cost performance.

Description

Technical Field

[0001] The present invention belongs to the field of nanomaterial technology, and specifically relates to a nano-functionalized antibacterial and oil-removing water-free cleaning wipe and its preparation method and application, which can be applied to special scenarios (high temperature, high humidity, high salt and non-open space). Background Art

[0002] Bathing is typically done with shampoo, shower gel, and water. However, in many situations, clean water is scarce or inconvenient, such as on ocean-going vessels where water supplies are limited and bathing is difficult; bedridden patients who cannot shower with water; and personnel in specialized occupations, such as outdoor workers and polar expeditions, who find it difficult to bathe with water. Therefore, waterless cleaning products have promising market prospects and a broad market (ABIHPEC, Economist Intelligence Unit, Japan Hygiene Products Industry Association, Fuji-Keizai Group, Information Resources, Inc., Alimarket, InfoScan Census™, Mintel). According to reports, in 2015, the global bath product market was led by the United States, with sales of $5.58 billion; China ranked second, with sales of $4.14 billion. According to the United Nations, by 2025, 1.8 billion people worldwide will be living in water-scarce environments. Given the busy and fast-paced lives of modern people, waterless bath products can save them time. Furthermore, with the global aging population, specialized bath products for seniors are filling a significant market gap.

[0003] Currently, most skin surface cleansing and disinfecting wipes on the market are limited to 75% alcohol formulas. While the antibacterial properties of 75% alcohol are widely recognized, the safety and risks associated with these wipes should not be underestimated. First, alcohol is a flammable and explosive chemical, requiring strict storage and transportation conditions. Exposure to open flames or high temperatures can cause combustion or explosion. Second, alcohol disinfection utilizes its powerful penetrating properties to rapidly penetrate pathogens, dehydrating, denaturing, and coagulating their internal proteins, ultimately killing them. This process also rapidly dehydrates skin cells, leading to dryness, itchiness, and cracking. Alcohol can also cause skin allergies, so those allergic to alcohol should avoid using these wipes. As living standards improve, the application and demand for disposable cleansing wipes expand, leading to higher safety and environmental requirements for these products. Consumers are increasingly demanding natural, healthy, and safe disposable cleansing wipes. The development of new, functional, and waterless cleansing wipes is primarily achieved by modifying the wipe's formula. Current waterless cleaning products on the market suffer from issues such as incomplete cleaning, strong odor, and high alcohol content, resulting in negative user reviews. Furthermore, their antibacterial and decontamination abilities are significantly reduced or even lost in high-temperature, high-humidity environments. Therefore, there is an urgent need to develop a waterless cleaning product that is convenient to use, non-toxic, environmentally friendly, cost-effective, and suitable for non-open spaces exposed to high temperatures and humidity. Summary of the Invention

[0004] The purpose of the present invention is to provide a nano-functional antibacterial and oil-removing water-free cleaning towel.

[0005] Another object of the present invention is to provide a method for preparing the nano-functionalized antibacterial and oil-removing water-free cleaning wipes.

[0006] The third object of the present invention is to provide a use of the nano-functionalized antibacterial and oil-removing water-free cleaning towel in the preparation of hand towels, bath towels, etc. in a high temperature and high humidity environment.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect of the present invention, a nano-functionalized antibacterial and oil-removing water-free cleaning wipe is provided, wherein the cleaning wipe is made of a carrier and the antibacterial liquid in a mass ratio of 1:3 to 5 (preferably 1:3, 1:4, 1:5);

[0009] The antibacterial liquid is made of the following components in percentage by mass:

[0010] 0.1-0.3% surfactant, 8-12% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 1-15% aloe extract, 0.8-1.2% menthol solution, 0.18-0.22% chemical preservative, and the balance water are used to prepare 100g of antibacterial solution;

[0011] In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.18-0.3%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.000045-0.0006%, and the mass percentage of menthol is 0.045-0.055%.

[0012] Preferably, the antibacterial liquid is made of the following components in percentage by mass:

[0013] 0.1-0.3% surfactant, 8-12% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 1-15% aloe extract, 0.8-1.2% menthol solution, 0.18-0.22% chemical preservative, and the balance water are used to prepare 100g of antibacterial solution;

[0014] In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.2%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.000045-0.0005%, and the mass percentage of menthol is 0.045-0.055%.

[0015] Most preferably, the antibacterial liquid is made of the following components in percentage by mass:

[0016] 0.1% surfactant, 10% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 10% aloe extract, 1% menthol solution, 0.2% chemical preservative, and the balance water are used to prepare 100g of antibacterial solution;

[0017] In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.2%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.00005%, and the mass percentage of menthol is 0.05%.

[0018] Most preferably, the antibacterial liquid is made of the following components in percentage by mass:

[0019] 0.2% surfactant, 10% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 10% aloe extract, 1% menthol solution, 0.2% chemical preservative, and the balance water are used to prepare 100g of antibacterial solution;

[0020] In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.2%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.0005%, and the mass percentage of menthol is 0.05%.

[0021] Most preferably, the antibacterial liquid is made of the following components in percentage by mass:

[0022] 0.3% surfactant, 10% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 10% aloe extract, 1% menthol solution, 0.2% chemical preservative, and the balance water are used to prepare 100g of antibacterial solution;

[0023] In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.2%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.0005%, and the mass percentage of menthol is 0.05%.

[0024] More preferably, the carrier is selected from functionalized non-woven fabrics.

[0025] The method for preparing the matrine nanoparticle solution comprises the following steps: dissolving matrine nanoparticles in deionized water to obtain the matrine nanoparticle solution.

[0026] The preparation method of the polylactic-co-glycolic acid modified carboxyl capsaicin nanoparticle solution comprises the following steps:

[0027] The polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles are dissolved in propylene glycol, Tween 80 is added, and the mixture is stirred evenly to obtain the polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticle solution.

[0028] The preparation method of the menthol solution comprises the following steps:

[0029] Menthol is dissolved in propylene glycol, and Tween 80 is added after the menthol is completely dissolved to obtain the menthol solution.

[0030] The method for preparing the functionalized non-woven fabric comprises the following steps:

[0031] The non-woven fabric is immersed in a hydrophilic functionalized cationic solution for 10 to 40 minutes (preferably 30 minutes), and dried at a temperature of 70 to 90° C. (preferably 80° C.) for 2 to 4 hours (preferably 3 hours) to obtain a functionalized non-woven fabric.

[0032] The non-woven fabric is selected from cotton fiber non-woven fabric, spunlace non-woven fabric, bamboo fiber non-woven fabric, all-viscose non-woven fabric, and wood pulp composite non-woven fabric.

[0033] The surfactant is selected from cocamidopropyl betaine, sodium lauroyl sarcosinate, potassium cocoyl glutamate, alkyl glucoside, PEG-40 castor oil, and Tween 80.

[0034] The chemical preservative is selected from chlorhexidine digluconate, sodium hydroxymethylglycinate, ethyl hydroxybenzoate, anisic acid and the like.

[0035] The particle size of the matrine nanoparticles is 100±10 nm.

[0036] The water is selected from deionized water, distilled water, and water for injection.

[0037] The preparation method of the matrine nanoparticles comprises the following steps:

[0038] Dissolve matrine and melted glyceryl monostearate in ethanol at a mass ratio of 1:1 to 3 (preferably 1:2) and stir at 70±2°C for 1 to 8 hours (preferably 5 hours) to prepare an organic phase;

[0039] Dissolve poloxamer and lecithin in a mass ratio of 1 to 16:1 (preferably 12:1) in deionized water, stir at 70±2°C for 1 to 8 hours (preferably 2 hours) to prepare an aqueous phase;

[0040] The organic phase is quickly injected into the aqueous phase, the volume ratio of the organic phase to the aqueous phase is 1:1 to 10 (preferably 1:5), and stirred at a temperature of 70±2°C for 10 to 60 minutes (preferably 50 minutes). The organic solvent is removed to form a nanoemulsion, which is injected into water at a temperature of 2 to 6°C (preferably 4°C), stirred for 1 to 8 hours (preferably 3 hours), and freeze-dried to obtain matrine nanoparticles.

[0041] The preparation method of the polylactic-co-glycolic acid modified carboxyl capsaicin nanoparticles comprises the following steps:

[0042] The polylactic acid glycolic acid modified carboxyl group and capsaicin in a mass ratio of 1 to 3:1 (preferably 1.7:1) are mixed and dissolved in ethyl acetate, and the resulting solution is added dropwise to a polyvinyl alcohol (PVA) aqueous solution with a concentration of 1 to 8% (preferably 5%); the volume ratio of the solution of the polylactic acid glycolic acid modified carboxyl group and capsaicin dissolved in ethyl acetate to the volume ratio of the polyvinyl alcohol aqueous solution is 1 to 5:1 (preferably 1.5:1); the emulsion is sonicated on ice at least 3 times, and then slowly added to a polyvinyl alcohol (PVA) aqueous solution with a concentration of 0.1 to 0.8% (preferably 0.3%), and the volume ratio of the solution of the polylactic acid glycolic acid modified carboxyl group and capsaicin dissolved in ethyl acetate to the volume ratio of the polyvinyl alcohol aqueous solution is 1:1; stirred at room temperature for 1 to 8 hours (preferably 6 hours), ethyl acetate is evaporated to obtain particles, centrifuged for the first time, washed at least twice in sterile deionized water, centrifuged a second time, resuspended in sterile deionized water, quick-frozen in liquid nitrogen, and then freeze-dried for at least 2 days to obtain polylactic acid glycolic acid modified carboxyl capsaicin nanoparticles.

[0043] The conditions of the first centrifugation were: centrifugation at 10,000 rpm for 20 minutes.

[0044] The conditions for the second centrifugation were: centrifugation at 10,000 rpm for 20 minutes.

[0045] The second aspect of the present invention provides a method for preparing the nano-functionalized antibacterial and oil-removing water-free cleaning wipes, comprising the following steps:

[0046] Immersing the carrier in an antibacterial liquid to obtain the nano-functionalized antibacterial, oil-removing, water-free cleaning wipe; the mass ratio of the carrier to the antibacterial liquid is 1:3 to 5 (preferably 1:3, 1:4, 1:5);

[0047] The preparation method of the antibacterial liquid comprises the following steps:

[0048] The antibacterial liquid is obtained by dissolving matrine nanoparticle solution, polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, aloe extract, menthol solution, surfactant and chemical preservative in water.

[0049] The third aspect of the present invention provides a use of the nano-functionalized antibacterial and oil-removing water-free cleaning towel in the preparation of hand towels, bath towels, etc. in a high-temperature and high-humidity environment.

[0050] The high temperature and high humidity environment refers to a temperature of 40° C. or higher and a humidity of 70 RH% or higher, specifically a temperature of 40-100° C. and a humidity of 70-90 RH%.

[0051] The present invention utilizes a non-open space simulation platform to simulate a high temperature and high humidity environment. Testing is performed according to the provisions of the "Technical Specifications for Disinfection (2002 Edition)" published by the Ministry of Health of the People's Republic of China. After use, the total bacterial colony count and the total fungal colony count of the cleaning wipes of the present invention should meet the requirements. No coliform group, Pseudomonas aeruginosa, Staphylococcus aureus, or hemolytic streptococci should be detected. The logarithmic killing value for Staphylococcus aureus should be ≥5.00, the logarithmic killing value for Pseudomonas aeruginosa should be ≥5.0, the logarithmic killing value for Candida albicans should be ≥5.00, and the inhibition rate for Aspergillus niger should be ≥90%. This product can be determined to be qualified for disinfection of fungal contaminants. It meets the requirements of the "Technical Specifications for Disinfection (2002 Edition)." Furthermore, the cleaning wipes of the present invention, when tested according to the provisions of the National Standard of the People's Republic of China "Wet Wipes" (GBT 27728-2011), have a higher oil removal capacity than the standard control solution.

[0052] Due to the adoption of the above technical solution, the present invention has the following advantages and beneficial effects:

[0053] The nano-functionalized antibacterial and oil-removing water-free cleaning towel provided by the present invention comprises a non-woven fabric carrier and an antibacterial liquid soaked on the substrate. The antibacterial liquid adopts a safe and non-toxic nano-functionalized Chinese medicine extract, and modifies the surface of the non-woven fabric, changes the roughness and anchor points of its surface, so that the antibacterial and oil-removing nano-monomers in the antibacterial liquid firmly "grasp" the surface of the functionalized non-woven fabric, increases the cleaning strength and efficacy, and the antibacterial and oil-removing efficacy is still significantly effective in a high temperature and high humidity environment (40-60°C, 70-90%RH). All raw materials of the present invention are selected from the national safety catalog, are safe and non-toxic, non-irritating, do not contain alcohol, are safe to the human body, have no side effects, can clean and sterilize the skin, and more importantly, are suitable for non-open spaces with high temperature and high humidity and do not need to be washed with water.

[0054] To address the harsh working environments and limited water resources of non-open spaces, the present invention provides a nano-functionalized antibacterial, oil-removing, waterless cleaning wipe with both cleaning and antibacterial properties, thereby meeting the personalized, quick cleaning needs of personnel in special scenarios. This invention overcomes the problems of existing waterless cleaning wipes, such as irritating ingredients, poor feel, weak detergency, and a narrow antibacterial range. The present cleaning wipe is convenient to use, non-toxic, environmentally friendly, and cost-effective.

[0055] This nanotechnology-based antibacterial, oil-removing, water-free cleaning wipe perfectly solves the problems of conventional disinfectants. It uses nanomaterials to sterilize via oxidative stress, maintaining excellent results with repeated use (even after 10 uses, the sterilization rate remains above 75%). The nanomaterials have a large surface area and are highly active, generating oxygen free radicals and reactants that can eliminate common pathogens, fungi, and drug-resistant strains, with a 99.9% kill rate against Staphylococcus aureus, Candida albicans, and Escherichia coli. Furthermore, it uses pure water with no additives, is gentle and non-irritating, and contains no residual or harmful chemicals, making it safe for human use.

[0056] The present invention uses nanotechnology to nano-functionalize antibacterial monomers, which not only increases the specific surface area of ​​the nanomedicine, but also increases the adsorption rate and improves the cleaning efficiency.

[0057] The antibacterial, oil-removing, water-free cleaning wipes of the present invention contain natural active substances from plants (Sophora flavescens extract, Aloe vera extract) that are mild and non-irritating. When added to the cleaning wipes, the product is suitable for various skin types. However, the natural plant ingredients are unstable. The present invention reduces the irritation of the cleaning wipes to the skin by making a nanoemulsion from water-insoluble plant ingredients and adding the nanoemulsion to the cleaning wipes, thereby improving the feel of use of the cleaning wipes and enhancing the antibacterial properties. The antibacterial liquid prepared by the present invention is a nanoemulsion, which is a colloidal dispersion system formed by an aqueous phase, an oil phase, and an emulsifier. The nanoemulsion has a small droplet size, generally 5-1000 nm, and can maintain dynamic stability for a long time without significant flocculation and aggregation. At the same time, it has a low oil / water interfacial tension. As a carrier of biologically active substances, it can encapsulate lipophilic active ingredients in nanodroplets, reducing the contact of the active ingredients with oxygen, thereby improving their stability, thereby increasing the utilization rate of the active ingredients by the skin and enhancing the barrier function of the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 This is a schematic diagram of the process for preparing nano-functionalized antibacterial and oil-removing water-free cleaning wipes.

[0059] Figure 2 Schematic diagram of the particle size results of nanoparticles in antibacterial liquid.

[0060] Figure 3 Schematic diagram of the particle size of the antibacterial solution.

[0061] Figure 4 It is a schematic diagram of the results of inhibiting Staphylococcus aureus by three commercially available products and the liquid in the cleaning towel prepared in Example 1. DETAILED DESCRIPTION

[0062] In order to explain the present invention more clearly, the present invention is further described below in conjunction with preferred embodiments. Those skilled in the art should understand that the following specific description is illustrative rather than restrictive and should not be used to limit the scope of protection of the present invention.

[0063] Preliminary screening experiments:

[0064] 1. Screening experiment of matrine nanoparticle dosage (tested at room temperature)

[0065] 1. The mass of matrine nanoparticles contained in 100g of antibacterial liquid is 0.1g, 0.2g, and 0.3g.

[0066] (1) Equipment

[0067] (1) Sample information: 100 g of antibacterial solution containing 0.1 g, 0.2 g, and 0.3 g of matrine nanoparticles were used as the test substance;

[0068] (2) Test strains: Aspergillus niger (ATCC16404) and Trichophyton rubrum (BNCC313807), provided by Beina Chuanglian Biotechnology Co., Ltd., cultured at the 4th generation;

[0069] (3) Culture medium: MEA / 20240611 for Aspergillus niger and SDA / 20240612 for Trichophyton rubrum;

[0070] (4) Instruments and equipment: water bath DC0510 (WPE-RH0327), biological safety cabinet BSC-1604ⅡB2 (WPE-RH0311), mold incubator MJ-250-I (WPE-RH0243).

[0071] (2) Methods

[0072] (1) Test basis: WS / T 650-2019 “Evaluation methods for antibacterial and antibacterial effects” 5.1.1 Quantitative antibacterial test for suspension.

[0073] (2) Testing environment: temperature: 22.7°C, humidity: 54% RH.

[0074] (3) Antibacterial test: The test substance was exposed to the bacteria for 5 min, 10 min, and 15 min, and the test was repeated three times.

[0075] (3) Conclusion

[0076] 1. The results of the quantitative antibacterial test of 100g of antibacterial solution containing 0.1g of matrine nanoparticles against Aspergillus niger suspension are shown in Table 1:

[0077] Table 1 Quantitative antibacterial test results of Aspergillus niger suspension

[0078]

[0079] After three repeated tests, the sample (100g antibacterial liquid containing 0.1g matrine nanoparticles) had an antibacterial rate of 33.8%, 31.3% and 27.4% against Aspergillus niger after 5min; an antibacterial rate of 41.9%, 39.2% and 37.2% against Aspergillus niger after 10min; and an antibacterial rate of 45.1%, 43.1% and 39.2% against Aspergillus niger after 15min, which did not meet the requirements of 5.1.1.5 of WS / T 650-2019 "Methods for Evaluation of Antibacterial and Antibacterial Effects".

[0080] The results of the quantitative antibacterial test of 100 g of antibacterial solution containing 0.1 g of matrine nanoparticles against a suspension of Trichophyton rubrum are shown in Table 2:

[0081] Table 2 Results of quantitative antibacterial test of suspension of Trichophyton rubrum

[0082]

[0083] After three repeated tests, the sample (100g antibacterial liquid containing 0.1g matrine nanoparticles) was acted for 5min, 10min, and 15min respectively, and the inhibition rate against Trichophyton rubrum was ≥90%, which showed a strong antibacterial effect and met the requirements of 5.1.1.5 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects".

[0084] 2. The results of the quantitative antibacterial test of 100g of antibacterial solution containing 0.2g of matrine nanoparticles against Aspergillus niger suspension are shown in Table 3:

[0085] Table 3 Quantitative antibacterial test results of suspension of Aspergillus niger

[0086]

[0087] After three repeated tests, the sample (100g antibacterial liquid containing 0.2g matrine nanoparticles) was acted for 5min, 10min, and 15min respectively, and the inhibition rate against Aspergillus niger was ≥90%, which showed a strong antibacterial effect and met the requirements of 5.1.1.5 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects".

[0088] The results of the quantitative antibacterial test of 100 g of antibacterial solution containing 0.2 g of matrine nanoparticles against a suspension of Trichophyton rubrum are shown in Table 4:

[0089] Table 4 Quantitative antibacterial test results of suspension of Trichophyton rubrum

[0090]

[0091] After three repeated tests, the sample (100g antibacterial liquid containing 0.2g matrine nanoparticles) was acted for 5min, 10min, and 15min respectively, and the inhibition rate against Trichophyton rubrum was ≥90%, which showed a strong antibacterial effect and met the requirements of 5.1.1.5 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects".

[0092] 3. The results of the quantitative antibacterial test of 100g of antibacterial solution containing 0.3g of matrine nanoparticles against Aspergillus niger suspension are shown in Table 5:

[0093] Table 5 Quantitative antibacterial test results of suspension of Aspergillus niger

[0094]

[0095]

[0096] After three repeated tests, the sample (100g antibacterial liquid containing 0.3g matrine nanoparticles) was acted for 5min, 10min, and 15min respectively, and the inhibition rate against Aspergillus niger was ≥90%, which showed a strong antibacterial effect and met the requirements of 5.1.1.5 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects".

[0097] Table 6 Quantitative antibacterial test results of suspension of Trichophyton rubrum

[0098]

[0099] After three repeated tests, the sample (100g antibacterial liquid containing 0.3g matrine nanoparticles) was acted for 5min, 10min, and 15min respectively, and the inhibition rate against Trichophyton rubrum was ≥90%, which showed a strong antibacterial effect and met the requirements of 5.1.1.5 of WS / T 650-2019 "Evaluation Methods for Antibacterial and Antibacterial Effects".

[0100] 100g of antibacterial liquid containing 0.2g and 0.3g of matrine nanoparticles has an inhibition rate of ≥90% against Trichophyton rubrum and Aspergillus niger, which meets the requirements of WS / T650-2019.

[0101] 2. Screening of the dosage of capsaicin-PLGA-COOH nanoparticles

[0102] The concentration of capsaicin added to skin care products varies depending on national and regional regulations, but generally does not exceed 0.025%.

[0103] The present invention's initial formulation has a capsaicin concentration of 0.02%, which produces a noticeable pungent sensation upon trial. The present invention also modifies capsaicin to produce capsaicin-PLGA-COOH nanoparticles, adjusting their mass percentage to 0.00005%. Trial use reveals no noticeable irritation. The modified capsaicin-PLGA-COOH nanoparticles act as an antibacterial agent, demonstrating a strong antifungal effect. References include the "Safety Technical Specifications for Cosmetics," relevant FDA guidelines, and EU Regulation EC No. 1223 / 2009.

[0104] Examples 1 to 3

[0105] Table 7 shows the components and dosage of 100g of antibacterial solution:

[0106] Table 7

[0107] Raw material name Example 1 Example 2 Example 3 Cocamidopropyl Betaine (g) 0.1 0.2 0.3 Matrine nanoparticles (g) 0.2 0.2 0.2 Capsaicin-PLGA-COOH nanoparticles (g) 0.00005 0.0005 0.0005 Aloe vera extract (g) 10 10 10 Menthol (g) 0.05 0.05 0.05 Chlorhexidine digluconate (g) 0.2 0.2 0.2 Pure water (g) 78.7 78.6 78.5

[0108] The raw materials used in the examples of the present invention are as follows: matrine was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd., batch number: 230630; cocamidopropyl betaine was purchased from Shanghai Yuanye Biotechnology Co., Ltd., batch number: J22HS174987; capsaicin was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd., batch number: 230816; aloe extract was purchased from Shanghai Baiyu Biotechnology Co., Ltd.; menthol was purchased from Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd., batch number: 230827; chlorhexidine digluconate was purchased from Aladdin Reagent Co., Ltd., batch number: E2416044; the water used in the examples of the present invention refers to deionized water.

[0109] A method for preparing a nano-functionalized antibacterial and oil-removing water-free cleaning wipe comprises the following steps: Figure 1 This is a flow chart of the preparation method of nano-functionalized antibacterial and oil-removing water-free cleaning wipes. ):

[0110] In the first step, in order to improve the therapeutic effect and bioavailability of matrine drugs, matrine nanoparticles were prepared by emulsification evaporation-low temperature solidification method, and the prepared matrine nanoparticles were freeze-dried:

[0111] The matrine nanoparticles were dissolved in deionized water to obtain a matrine nanoparticle solution with a concentration of 20 mg / mL.

[0112] The preparation method of the matrine nanoparticles comprises the following steps:

[0113] Glyceryl monostearate (GMS) was melted in a water bath at 10°C, 0.5 g of matrine and 1 g of the melted glyceryl monostearate were dissolved in 10 mL of ethanol, and the mixture was stirred in a water bath at 70±2°C for 5 h to prepare an organic phase.

[0114] Dissolve 1.2 g of poloxamer and 0.1 g of lecithin in 20 mL of deionized water, stir in a water bath at 70 ± 2 °C for 2 h to prepare an aqueous phase.

[0115] The organic phase was quickly injected into the aqueous phase, stirred at 70±2°C for 50 min, and the organic solvent was removed to form a nanoemulsion, which was then injected into 10 mL of water at 4°C, stirred for 3 h, and freeze-dried in a freeze dryer to obtain 0.5-0.7 g of matrine nanoparticles. The particle size results were as follows: Figure 2 As shown in a, Figure 2 Schematic diagram of the particle size results of nanoparticles in the antibacterial liquid, wherein a is a schematic diagram of the particle size of matrine nanoparticles; it can be seen from the figure that the particle size of matrine nanoparticles is between 100±10nm.

[0116] The second step is to prepare polylactic acid glycolic acid modified carboxyl capsaicin nanoparticles (abbreviated as capsaicin-PLGA-COOH nanoparticles) comprising the following steps:

[0117] Capsaicin is modified with nano-functionalization. However, due to its strong irritation, strong odor, and low water solubility, its application is limited. Therefore, the present invention develops a multilayer nanoemulsion containing polylactic-co-glycolic acid modified carboxyl groups to mask its odor and taste and improve its physicochemical stability to the surrounding environment.

[0118] 100 mg of polylactic acid glycolic acid modified carboxyl (PLGA-COOH) (Shenzhen Weihua Biotechnology Co., Ltd., 95%) and 60 mg of capsaicin were mixed and dissolved in 3 mL of ethyl acetate, and the resulting solution was added dropwise to 2 mL of 5% polyvinyl alcohol (PVA) aqueous solution. The emulsion was sonicated on ice 3 times for 10 seconds each time, and then slowly added to a beaker containing 3 mL of 0.3% polyvinyl alcohol (PVA) aqueous solution, stirred at room temperature for 6 hours, and ethyl acetate was evaporated under a fume hood to harden the particles. The particles were collected, centrifuged at 10,000 rpm for 20 minutes, washed twice in sterile deionized water to remove unencapsulated capsaicin, centrifuged at 10,000 rpm for 20 minutes, resuspended in 5 mL of sterile deionized water, quick-frozen in liquid nitrogen, and then the nanoparticles were further freeze-dried for 2 days using a freeze dryer to obtain polylactic acid glycolic acid modified carboxyl capsaicin nanoparticles (capsaicin-PLGA-COOH nanoparticles), which were stored at -20°C. The particle size results are as shown in the figure. Figure 2 As shown in b, Figure 2 Figure 2 is a schematic diagram of the particle size results of nanoparticles in the antibacterial solution, where (b) is a schematic diagram of the particle size of capsaicin-PLGA-COOH nanoparticles. As can be seen from the figure, the particle size of capsaicin-PLGA-COOH nanoparticles is between 280±10nm.

[0119] 52.5 mg of capsaicin-PLGA-COOH nanoparticles were dissolved in 1 mL of propylene glycol, 1 mL of Tween 80 was added (Tween 80 was added as a solubilizer to increase the solubility of capsaicin-PLGA-COOH nanoparticles), and the mixture was stirred evenly to obtain a capsaicin-PLGA-COOH nanoparticle solution.

[0120] The third step, the preparation method of the menthol solution comprises the following steps:

[0121] 100 mg of menthol was dissolved in 2 mL of propylene glycol. After complete dissolution, 2 mL of Tween 80 (as a solubilizer to increase the solubility of menthol) was added to obtain a menthol solution.

[0122] The fourth step is to functionalize the non-woven fabric:

[0123] Cotton fiber non-woven fabric (purchased from Ningbo Shuocai New Materials Co., Ltd., thickened> 80g / m 2 , 70cm×140cm) was immersed in 100mL of hydrophilic functionalized cationic solution (Guangzhou Lianzhuang Technology Co., Ltd., model: N09 non-woven fabric hydrophilic agent), soaked for 30 minutes, and dried in an oven at a temperature of 80°C for 3 hours to obtain a functionalized non-woven fabric.

[0124] Functionalized nonwovens have excellent hygroscopicity (i.e., hydrophilicity). The cations form anchor points on the surface, forming a continuous, hydrophilic membrane on the fiber surface. Like hooks, the functionalized nonwovens can firmly grasp the active ingredients in the antibacterial solution, increasing the liquid content. Furthermore, the functionalized nonwovens increase their surface roughness, further enhancing their degreasing effectiveness.

[0125] In the fifth step, the preparation method of the nano-functionalized antibacterial and oil-removing water-free cleaning wipes of Example 1 comprises the following steps:

[0126] The preparation method of the antibacterial liquid comprises the following steps: dissolving 10 mL of the 20 mg / mL matrine nanoparticle solution prepared in the first step, 1 μL of the 52.5 mg / mL capsaicin-PLGA-COOH nanoparticle solution prepared in the second step, 10 g of aloe extract, 1 mL of the 50 mg / mL menthol solution prepared in the third step, 0.1 g of cocamidopropyl betaine, and 0.2 g of chlorhexidine digluconate in 78.7 mL of deionized water to obtain 100 g of the antibacterial liquid. Figure 3 Figure 2 is a schematic diagram of the particle size of the antibacterial liquid. As can be seen from the figure, the average particle size in the antibacterial liquid is 257nm.

[0127] 10 g of the functionalized non-woven fabric was immersed in 40 g of the antibacterial solution and sealed into a bag using a Fresh World vacuum sealer (model: 3150) to obtain a nano-functionalized antibacterial and oil-removing water-free cleaning towel.

[0128] Example 2

[0129] The preparation method of the nano-functionalized antibacterial and oil-removing water-free cleaning wipes comprises the following steps:

[0130] The preparation method of the antibacterial liquid comprises the following steps: dissolving 10 mL of the 20 mg / mL matrine nanoparticle solution prepared in the first step, 10 μL of the 52.5 mg / mL capsaicin-PLGA-COOH nanoparticle solution prepared in the second step, 10 g of aloe extract, 1 mL of the 50 mg / mL menthol solution prepared in the third step, 0.2 g of cocamidopropyl betaine, and 0.2 g of chlorhexidine digluconate in 78.6 mL of deionized water to obtain 100 g of the antibacterial liquid.

[0131] 10 g of the functionalized non-woven fabric was immersed in 50 g of antibacterial liquid and sealed into a bag using a Fresh World vacuum sealer (model: 3150) to obtain a nano-functionalized antibacterial and oil-removing water-free cleaning towel.

[0132] Example 3

[0133] The preparation method of the nano-functionalized antibacterial and oil-removing water-free cleaning wipes comprises the following steps:

[0134] The preparation method of the antibacterial liquid comprises the following steps: dissolving 10 mL of the 20 mg / mL matrine nanoparticle solution prepared in the first step, 10 μL of the 52.5 mg / mL capsaicin-PLGA-COOH nanoparticle solution prepared in the second step, 10 g of aloe extract, 1 mL of the 50 mg / mL menthol solution prepared in the third step, 0.3 g of cocamidopropyl betaine, and 0.2 g of chlorhexidine digluconate in 78.5 mL of deionized water to obtain 100 g of the antibacterial liquid.

[0135] 10 g of the functionalized non-woven fabric was immersed in 30 g of antibacterial liquid and sealed into a bag using a Fresh World vacuum sealer (model: 3150) to obtain a nano-functionalized antibacterial and oil-removing water-free cleaning towel.

[0136] Example 4

[0137] The prepared nano-functionalized antibacterial and oil-removing water-free cleaning wipes were tested and evaluated on a high-temperature and high-humidity climate chamber platform (purchased from Shanghai Lichen Instrument Technology Co., Ltd., LC-HN-40S).

[0138] A constant temperature and humidity climate chamber is used to simulate a high temperature and high humidity environment (40-60°C, 70-90RH%) for microbiological experiments, including: inhibition zone, plate colony count method, turbidity method, bacterial growth determination and animal experiments.

[0139] According to the "Technical Specifications for Disinfection (2002 Edition)" published by the Ministry of Health of the People's Republic of China, the total bacterial and fungal colony counts must meet the requirements. No coliform bacteria, Pseudomonas aeruginosa, Staphylococcus aureus, or hemolytic Streptococcus must be detected. The logarithmic killing value for Staphylococcus aureus should be ≥5.00, the logarithmic killing value for Pseudomonas aeruginosa should be ≥5.0, the logarithmic killing value for Candida albicans should be ≥5.00, and the inhibition rate against Aspergillus niger should be ≥90%. This product can be determined to be qualified for the disinfection of fungal contaminants and meets the requirements of the "Technical Specifications for Disinfection (2002 Edition)."

[0140] Pollutants in the air can affect the expression of cellular genes. Air pollution can stimulate the transcription of pro-inflammatory and immunomodulatory cytokines, change the expression of important proteins in cell differentiation, and affect the formation of wrinkles and spots. Studies have found that exposure to air pollutants can significantly upregulate the expression of genes in keratinocytes, including cytokines IFN-γ, IL-4, TGF-β1, IL-9, and IL-17. In order to verify whether the use of nano-functionalized antibacterial and oil-removing water-free cleaning wipes can effectively remove pollutants from the body surface under the special environment of high temperature and high humidity. The present invention selected 6-week-old male BALbc mice, removed the hair on the back, and placed them in a high temperature and high humidity non-open space cabin (40°C, 70RH%). The cleaning wipe group: wiped the back with the nano-functionalized antibacterial and oil-removing water-free cleaning wipes prepared by the present invention every day for 30 seconds. The blank group did not receive any treatment. After 3 consecutive days of swabbing, blood was collected from the eye sockets, serum was separated, and back skin tissue was collected. Serum and tissue samples were tested for IFN-γ, IL-4, TGF-β1, IL-9, and IL-17 Elisa.

[0141] Serum samples: Collect in sterile tubes, allow blood to coagulate naturally at room temperature for 10-20 minutes, centrifuge at 2-8°C for about 20 minutes (2000-3000 rpm), and carefully collect the supernatant. If precipitation occurs during storage, centrifuge again.

[0142] Tissue specimens: After cutting the specimen, weigh 1g of tissue, add 9g of PBS with a pH of about 7.2-7.4, and homogenize the specimen thoroughly by hand or with a homogenizer. Centrifuge for about 20 minutes (2000-3000 rpm) and carefully collect the supernatant. Aliquot a portion for testing and freeze the rest for later use. If a precipitate is formed during storage, centrifuge again. After wiping with the wet wipes, the levels of IFN-γ, IL-9, and IL-17 in mouse serum increased, while the levels of IL-4 and TGF-β1 decreased. After wiping, the factors related to comfort in the mouse blood samples increased. The results are shown in Table 4.

[0143] The nano-functionalized antibacterial, oil-removing, water-free cleaning wipes prepared in Examples 1 to 3 were tested for their good bactericidal properties under high temperature and humidity conditions, in accordance with the requirements of the Technical Specifications for Disinfection (2002 edition). The following items were tested: total bacterial colony count, total fungal colony count, coliform bacteria, Pseudomonas aeruginosa, Staphylococcus aureus, and hemolytic Streptococcus; none were detected, and all test results met the standards. Bactericidal properties against pathogenic microorganisms including Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus niger were also tested. The results are shown in Table 8. The logarithmic killing values ​​for Staphylococcus aureus and Pseudomonas aeruginosa were both ≥5.00, meeting the requirements of 2.1.1.7.7 of the Technical Specifications for Disinfection (2002 edition). The logarithmic killing values ​​for Candida albicans and Aspergillus niger were both ≥4.00, meeting the requirements of 2.1.1.9.6 of the Technical Specifications for Disinfection (2002 edition).

[0144] Table 8

[0145] Technical indicators Example 1 Example 2 Example 3 Killing logarithm value (Staphylococcus aureus) ≥5.00 ≥5.00 ≥5.00 Killing logarithm value (Pseudomonas aeruginosa) ≥5.00 ≥5.00 ≥5.00 Killing logarithm value (Candida albicans) ≥4.00 ≥4.00 ≥4.00 Killing logarithm (Aspergillus niger) ≥4.00 ≥4.00 ≥4.00

[0146] According to the provisions of the National Standard of the People's Republic of China "Wet Wipes" (GBT 27728-2011), the degreasing ability of the disposable cleaning wipes was evaluated in a high temperature and high humidity environment (40°C, 70RH%). The results are shown in Table 9:

[0147] Table 9

[0148]

[0149] If the oil removal rate of the test solution is equal to or greater than that of the standard solution, the sample is judged to have qualified detergency; otherwise, it is judged to be unqualified. The nano-functionalized antibacterial oil-removing, waterless cleaning wipes of the present invention have a higher oil removal rate than the standard control solution. This indicates that the cleaning wipes prepared in Examples 1-3 all have a higher oil removal rate than the standard solution group, and therefore are judged to have qualified detergency.

[0150] Table 10 Test results of mouse comfort-related factors under high temperature and high humidity environment (40°C, 70RH%) (Example 1)

[0151]

[0152] From the data in Table 10, it can be seen that the levels of IFN-γ, IL-9 and IL-17 in the serum of mice in the blank group increased, while the levels of IL-4 and TGF-β1 decreased. In the clean towel group, the factors related to comfort in the blood samples of mice increased after wiping, indicating that the comfort of the mice improved.

[0153] The antibacterial liquid prepared in Example 1 of the present invention was tested for its ability to disinfect harmful microorganisms on the body surface, and the specific results are shown in Table 11. The test subjects were 30 people, and the changes in colony counts before and after using the antibacterial liquid prepared in Example 1 of the present invention were analyzed.

[0154] 1. Equipment

[0155] 1. Sample information: The antibacterial solution prepared in Example 1 and placed under high temperature and high humidity conditions for 12 hours was used as the test substance.

[0156] 2. Neutralizing agent formula: D / E (D / E Neutralizing Broth) neutralizing broth.

[0157] 3. Culture medium: TSA / 20240311.

[0158] 4. Instruments and equipment: biological safety cabinet BSC-1604ⅡA2 (WPE-RH0310), electric constant temperature incubator DHP-9272 (WPE-RH0501).

[0159] 2. Methods

[0160] 1. Inspection basis: Technical Specifications for Disinfection (2002 edition) 2.1.2.8 Field test of skin disinfection with disinfectants.

[0161] 2. Testing environment: temperature: 21.6℃, humidity: 50%RH.

[0162] 3. Disinfection field test: Wipe the arm skin with the test substance for 3 minutes. Repeat the test 30 times.

[0163] Table 11

[0164]

[0165]

[0166] As can be seen from the data in Table 11, the antibacterial liquid prepared in Example 1 was used as a sample to wipe the arm skin for 3 minutes, and the average logarithmic killing value of natural bacteria on the skin surface of 30 batches of people was ≥1.00, which meets the requirements of 2.1.2.8.4 of the "Technical Specifications for Disinfection" (2002 edition).

[0167] Example 5

[0168] Three commercially available products and the cleaning towel prepared in Example 1 were selected for antibacterial comparison. The results are shown in Table 12.

[0169] Table 12

[0170] Group Diameter of inhibition zone (mm) blank 0 Market Cleaning Wipes A Wet Liquid 7 Market Cleaning Wipes B Wet Liquid 0 Market Cleaning Wipes C Wet Liquid 0 Cleaning towel prepared in Example 1 11

[0171] As the drug solution absorbed by the filter paper / cleaning wipe slowly diffuses, a clear zone of inhibition appears around the filter paper / cleaning wipe. The size of this zone indicates the antibacterial potency of the drug. Within a certain drug concentration range, the higher the drug concentration, the larger the diameter of the zone. This method is also suitable for measuring the antibacterial effect against individual bacteria.

[0172] Figure 4 The figure shows the results of the inhibition of Staphylococcus aureus by three commercially available products and the liquid in the cleaning wipes prepared in Example 1. The experimental results show that both the wet liquid in the commercial cleaning wipes A and the cleaning wipes prepared in Example 1 produced zones of inhibition with diameters ≥7 mm, indicating antibacterial activity. However, the diameter of the zones in the cleaning wipes prepared in Example 1 reached 11 mm, demonstrating superior efficacy. The wet liquid in the commercial cleaning wipes B and C, as well as the blank control group, produced no zones of inhibition.

[0173] The above description is merely a preferred embodiment of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as above in terms of a preferred embodiment, it is not intended to limit the present invention. Any technician familiar with this patent can make slight changes or modifications to equivalent embodiments using the above technical content without departing from the scope of the technical solution of the present invention. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the solution of the present invention.

Claims

1. A nano-functionalized antibacterial and oil-removing water-free cleaning towel, characterized in that: The cleaning towel is made of a carrier and the antibacterial liquid in a mass ratio of 1:3-5; The antibacterial liquid is made of the following components in percentage by mass: 0.1-0.3% surfactant, 8-12% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 1-15% aloe extract, 0.8-1.2% menthol solution, 0.18-0.22% chemical preservative, and the balance water, to prepare 100g antibacterial liquid; In the 100g antibacterial liquid, the mass percentage of matrine nanoparticles is 0.18-0.3%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.000045-0.0006%, and the mass percentage of menthol is 0.045-0.055%; The carrier is selected from functionalized non-woven fabrics.

2. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1, characterized in that: The antibacterial liquid is made of the following components in percentage by mass: 0.1-0.3% surfactant, 8-12% matrine nanoparticle solution, 0.000001-0.0008% polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, 1-15% aloe extract, 0.8-1.2% menthol solution, 0.18-0.22% chemical preservative, and the balance water, to prepare 100g antibacterial liquid; In 100 g of the antibacterial liquid, the mass percentage of matrine nanoparticles is 0.2%, the mass percentage of polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles is 0.000045-0.0005%, and the mass percentage of menthol is 0.045-0.055%.

3. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The method for preparing the functionalized nonwoven fabric comprises the following steps: The non-woven fabric is immersed in a hydrophilic functionalized cationic solution for 10 to 40 minutes, and dried at a temperature of 70 to 90° C. for 2 to 4 hours to obtain a functionalized non-woven fabric.

4. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The nonwoven fabric is selected from cotton fiber nonwoven fabric, spunlace nonwoven fabric, bamboo fiber nonwoven fabric, all-viscose nonwoven fabric, and wood pulp composite nonwoven fabric.

5. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The surfactant is selected from cocamidopropyl betaine, sodium lauroyl sarcosinate, potassium cocoyl glutamate, alkyl glucoside, PEG-40 castor oil, and Tween 80.

6. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The chemical preservative is selected from chlorhexidine digluconate, sodium hydroxymethylglycinate, ethylparaben, and anisic acid.

7. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The preparation method of the matrine nanoparticles comprises the following steps: Dissolve matrine and melted glyceryl monostearate in ethanol at a mass ratio of 1:1-3, stir for 1-8 hours at a temperature of 70±2°C, and prepare an organic phase; Dissolve poloxamer and lecithin in a mass ratio of 1 to 16:1 in deionized water, stir for 1 to 8 hours at a temperature of 70±2°C, and prepare an aqueous phase; The organic phase is rapidly injected into the aqueous phase, the volume ratio of the organic phase to the aqueous phase is 1:1-10, and the mixture is stirred for 10-60 min at a temperature of 70±2°C, the organic solvent is removed to form a nanoemulsion, and the mixture is injected into water at a temperature of 2-6°C, stirred for 1-8 h, and freeze-dried to obtain matrine nanoparticles.

8. The nano-functionalized antibacterial and oil-removing water-free cleaning wipe according to claim 1 or 2, characterized in that: The preparation method of the polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles comprises the following steps: The polylactic acid-glycolic acid modified carboxyl group and capsaicin in a mass ratio of 1 to 3:1 are mixed and dissolved in ethyl acetate, and the obtained solution is added dropwise to a polyvinyl alcohol aqueous solution with a concentration of 1 to 8%; the volume ratio of the solution of the polylactic acid-glycolic acid modified carboxyl group and capsaicin dissolved in ethyl acetate to the volume ratio of the polyvinyl alcohol aqueous solution is 1 to 5:1; the emulsion is ultrasonicated on ice for at least 3 times, and then slowly added to a polyvinyl alcohol aqueous solution with a concentration of 0.1 to 0.8%, and the volume ratio of the solution of the polylactic acid-glycolic acid modified carboxyl group and capsaicin dissolved in ethyl acetate to the volume ratio of the polyvinyl alcohol aqueous solution is 1:1; stirred at room temperature for 1 to 8 hours, ethyl acetate is evaporated, particles are obtained, centrifuged for the first time, washed at least twice in sterile deionized water, centrifuged for the second time, resuspended in sterile deionized water, quick-frozen in liquid nitrogen, and then freeze-dried for at least 2 days to obtain polylactic acid-glycolic acid modified carboxyl capsaicin nanoparticles.

9. A method for preparing the nano-functionalized antibacterial and oil-removing water-free cleaning wipes according to any one of claims 1 to 8, characterized in that: The following steps are involved: The carrier is immersed in an antibacterial liquid to obtain the nano-functionalized antibacterial oil-removing water-free cleaning towel; the mass ratio of the carrier to the antibacterial liquid is 1:3-5; The preparation method of the antibacterial liquid comprises the following steps: The antibacterial solution is obtained by dissolving matrine nanoparticle solution, polylactic acid glycolic acid modified carboxyl capsaicin nanoparticle solution, aloe extract, menthol solution, surfactant and chemical preservative in water.

10. Use of the nano-functionalized antibacterial and oil-removing water-free cleaning towel according to any one of claims 1 to 8 in preparing hand towels and bath towels in a high temperature and high humidity environment, characterized in that: The high temperature and high humidity environment refers to a temperature of 40° C. or higher and a humidity of 70 RH% or higher.

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