Fresh traditional Chinese medicine composition for pharyngitis and application

Abstract

The invention provides a traditional Chinese medicine fresh medicine composition for pharyngitis and application, and relates to the technical field of traditional Chinese medicine pharmacy, the traditional Chinese medicine fresh medicine composition is composed of fresh folium artemisiae argyi, fresh radix asparagi, fresh folium mori and fresh liquorice, and through the ingenious compatibility of monarch, minister, assistant and guide, the traditional Chinese medicine fresh medicine composition has the effects of clearing away heat and toxic materials, moistening dryness, promoting the secretion of saliva or body fluid and relieving swelling and pain; experimental data show that the traditional Chinese medicine fresh medicine composition has a remarkable anti-inflammatory effect, can remarkably improve the diet water intake, scratching frequency and pharyngeal mucosa hyperemia and redness and swelling phenomena of acute pharyngitis model rats, and improves throat tissue lesions. The fresh traditional Chinese medicine composition can be developed and utilized as a new medicine for preventing and treating pharyngitis.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine pharmaceutical preparation, and in particular to a traditional Chinese medicine fresh medicine composition for pharyngitis and application thereof. Background Art

[0002] Pharyngitis is an inflammatory disease of the pharyngeal mucosa and its underlying structures, including clinically diagnosed pharyngitis, tonsillopharyngitis, nasopharyngitis and other upper respiratory tract infection types. It usually presents as an acute course, but can also present as a subacute or chronic course. Acute pharyngitis is a common and frequently occurring disease in otolaryngology. It is an acute infectious disease of the pharyngeal mucosa, submucosa and related lymphoid tissue caused by bacterial, viral infection or physical and chemical factors. It is prone to occur in winter and spring and at the turn of the two seasons. Its clinical manifestations include cough, sore throat, hoarseness, fever, etc. Physical examination can reveal congestion of the pharyngeal mucosa, redness and swelling of the uvula, soft palate and posterior pharyngeal wall lymph follicles, and enlarged submandibular or submental lymph nodes.

[0003] Mr. Tu Youyou was inspired by the use of fresh Artemisia annua to fight malaria in the "Emergency Prescriptions for the Elbow", and discovered artemisinin, which promoted the research and application of fresh Chinese medicine by many scholars. Fresh Chinese medicine refers to fresh plants (including roots, stems, leaves, flowers, fruits, etc.), fresh animals (including whole animals or organs, tissues, etc.), etc. that have not been dried or processed. They are Chinese medicinal materials directly used to treat diseases under the guidance of traditional Chinese medicine theory. They are one of the characteristics of Chinese medicine treatment. Their application was first seen in the "Shennong Bencao Jing" that "fresh ones are especially good", and "fresh" here means "fresh". Because they do not undergo any drying and complex processing processes, unstable substances in fresh Chinese medicine, such as volatile and heat-sensitive components, do not undergo degradation, loss or change, the types and contents of chemical components are richer, and the natural ratios between components are not destroyed. Therefore, compared with its processed products, the original medicinal properties and efficacy of Chinese medicine are completely preserved, and its efficacy is more prominent when treating diseases.

[0004] Traditional Chinese medicine classifies pharyngitis as "acute throat paralysis". It is believed that acute throat paralysis is mostly caused by wind-heat evil toxins invading the throat, internally injuring the lungs, causing throat swelling and pain; or evil heat is congested, transmitted from the lungs to the inside, and heat evil burns the throat along the meridians, causing throat swelling and pain. The treatment principle is mainly to dispel wind and heat, promote lung function and relieve throat pain, and detoxify and moisten the throat. The existing drugs are not very ideal for the treatment of pharyngitis. Western medicine mainly uses anti-inflammatory and antiviral drugs, which can only alleviate symptoms to a certain extent, with insignificant efficacy, and are prone to drug resistance, adverse reactions, etc.; Chinese medicine is mostly lozenges and sprays, but it is also mainly used to alleviate local symptoms, with slow efficacy, large dosage, long treatment time, and not very ideal effect. Therefore, it is of great practical significance to develop a drug preparation that is quick-acting, effective, not prone to recurrence, and highly safe. Summary of the invention

[0005] 1. Technical issues to be resolved

[0006] In view of the deficiencies in the prior art, the present invention provides a fresh Chinese medicinal composition for pharyngitis and its application.

[0007] (II) Technical solution

[0008] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0009] The invention provides a traditional Chinese medicine fresh medicine composition for pharyngitis. The traditional Chinese medicine fresh medicine composition is composed of the following raw materials in parts by weight: 10-30 parts of fresh wormwood leaves, 1-15 parts of fresh asparagus cochinchinensis, 3-20 parts of fresh mulberry leaves and 1-15 parts of fresh liquorice.

[0010] Furthermore, the fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wormwood leaves, 1-10 parts of fresh asparagus cochinchinensis, 5-15 parts of fresh mulberry leaves, and 1-10 parts of fresh licorice.

[0011] Furthermore, the fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 20 parts of fresh wormwood leaves, 5 parts of fresh asparagus cochinchinensis, 10 parts of fresh mulberry leaves, and 5 parts of fresh licorice.

[0012] Further, in the Chinese herbal medicine fresh medicine composition, fresh wormwood leaf is the fresh leaf of Artemisia argyiLevl.et Vant. of Asteraceae; fresh asparagus is the fresh root of Asparagus cochinchinensis (Lour.) Merr. of Liliaceae; fresh mulberry leaf is the fresh leaf of Morus alba L. of Moraceae; fresh licorice is the fresh root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of Leguminosae. The above medicinal materials are freshly picked when used without any processing or preparation. The above medicinal materials are freshly picked when used without any processing or preparation.

[0013] The present invention provides a method for preparing a fresh Chinese medicinal composition for pharyngitis, comprising the following steps:

[0014] (1) Weigh fresh wormwood leaves, fresh asparagus cochinchinensis, fresh mulberry leaves, and fresh liquorice according to the above weight proportions, add appropriate amount of pure water to extract juice using a wall breaking machine, and filter to obtain a filtrate;

[0015] (2) The filtrate is frozen and vacuum concentrated to 0.5 g / mL.

[0016] The Chinese medicinal fresh drug composition provided by the present invention is used to prepare a drug for treating pharyngitis. The Chinese medicinal pharmacological effects of each raw material are as follows:

[0017] Fresh mugwort leaves: spicy and bitter in taste, cool in nature, enter the liver, spleen and kidney meridians; have the effects of clearing away heat and detoxifying, removing dampness and relieving itching. Fresh mugwort leaves can be used to treat vomiting blood and nosebleeds caused by blood heat, and itching caused by damp heat.

[0018] Fresh Asparagus cochinchinensis: sweet in taste, cold in nature, enters the lung and kidney meridians; has the effects of nourishing yin and moistening dryness, clearing the lungs and reducing fire, and is used for yin deficiency fever, cough and vomiting blood, pulmonary emphysema, lung abscess, sore throat, diabetes, and constipation.

[0019] Fresh mulberry leaves: sweet and bitter in taste, cold in nature, enter the lung and liver meridians; have the effects of dispersing wind-heat, clearing the lungs and moistening dryness, clearing the liver and improving eyesight, used for wind-heat colds, lung heat and dry coughs, dizziness and headaches, and red and blurred eyes.

[0020] Fresh licorice: sweet in taste, cool in nature, enters the spleen, lung, heart, and stomach meridians, has the effects of clearing away heat and detoxifying, removing phlegm and relieving cough, and is used for carbuncles, sore throat, cough with phlegm, etc.

[0021] (III) Beneficial effects

[0022] The invention provides a Chinese medicine fresh medicine composition and application for pharyngitis. The Chinese medicine fresh medicine composition is composed of four Chinese medicines, namely, fresh wormwood leaf, fresh asparagus cochinchinensis, fresh mulberry leaf and fresh licorice. Through the exquisite compatibility of monarch, minister, assistant and messenger, the composition aims to clear away heat and detoxify, moisten dryness and produce body fluid, reduce swelling and relieve pain, and effectively treat pharyngitis and sore throat. This prescription uses a large dose of fresh wormwood leaf as the monarch medicine, which has the effects of clearing heat and detoxifying, reducing swelling and relieving pain; uses fresh asparagus cochinchinensis as the minister medicine, which has the effects of moistening the lungs and producing body fluid, clearing away deficiency heat; uses fresh mulberry leaf as the adjuvant medicine, which has the effects of clearing lungs and reducing fire, moistening throat and removing phlegm, can penetrate into the lung system, complement each other with other medicines, and significantly improve the clearing and moistening effect of this prescription; uses fresh licorice as the messenger medicine, which is sweet in nature and can reconcile other medicines, balance the medicinal properties, and at the same time has the functions of moistening throat and relieving cough, detoxifying, and harmonizing the middle and relieving urgency, avoiding the feeling of greasy stagnation. The whole prescription compatibility provides the functions of clearing heat and detoxifying, moistening dryness and producing body fluid, reducing swelling and relieving pain.

[0023] The fresh Chinese medicinal composition of the present invention can inhibit the secretion of inflammatory factors TNF-α and IL-6 by macrophages RAW264.7 induced by LPS; can significantly improve the food and water intake, scratching frequency and pharyngeal mucosal congestion and swelling of rats with acute pharyngitis model, reduce serum TNF-α, IL-1β, IL-6, IL-10 and PGE2 levels, increase IFN-γ levels, effectively inhibit the release of inflammatory factors, reduce the level of inflammation, improve pharyngeal tissue lesions, and the therapeutic effect is better than that of its processed products and the Chinese medicine positive medicine Lanqin oral liquid. DETAILED DESCRIPTION

[0024] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1

[0026] A traditional Chinese medicine fresh medicine composition for pharyngitis is composed of the following raw materials in parts by weight: 20 parts of fresh wormwood leaves, 5 parts of fresh asparagus cochinchinensis, 10 parts of fresh mulberry leaves and 5 parts of fresh liquorice.

[0027] In the traditional Chinese medicine fresh medicine composition, fresh wormwood leaf is the fresh leaf of Artemisia argyi Levl.et Vant. of Asteraceae; fresh asparagus is the fresh root of Asparagus cochinchinensis (Lour.) Merr. of Liliaceae; fresh mulberry leaf is the fresh leaf of Morus alba L. of Moraceae; fresh licorice is the fresh root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of Leguminosae. The above medicinal materials are freshly picked when used without any processing or preparation.

[0028] The preparation method of the fresh Chinese medicinal composition comprises the following steps:

[0029] (1) Weigh fresh wormwood leaves, fresh asparagus cochinchinensis, fresh mulberry leaves, and fresh liquorice according to the above weight proportions, add appropriate amount of pure water to extract juice using a wall breaking machine, and filter to obtain a filtrate;

[0030] (2) Freeze and vacuum concentrate the filtrate to obtain the product.

[0031] Example 2

[0032] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 10 parts of fresh wormwood leaves, 1 part of fresh asparagus cochinchinensis, 3 parts of fresh mulberry leaves, and 1 part of fresh licorice.

[0033] Example 3

[0034] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 30 parts of fresh wormwood leaves, 15 parts of fresh asparagus cochinchinensis, 20 parts of fresh mulberry leaves, and 15 parts of fresh licorice.

[0035] Example 4

[0036] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 12 parts of fresh wormwood leaves, 3 parts of fresh asparagus cochinchinensis, 5 parts of fresh mulberry leaves, and 3 parts of fresh licorice.

[0037] Example 5

[0038] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 15 parts of fresh wormwood leaves, 4 parts of fresh asparagus cochinchinensis, 7 parts of fresh mulberry leaves, and 3 parts of fresh licorice.

[0039] Example 6

[0040] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 24 parts of fresh wormwood leaves, 7 parts of fresh asparagus cochinchinensis, 12 parts of fresh mulberry leaves, and 7 parts of fresh licorice.

[0041] Example 7

[0042] The difference between this embodiment and embodiment 1 is that the Chinese herbal medicine fresh medicine composition comprises the following raw materials in parts by weight: 28 parts of fresh wormwood leaves, 10 parts of fresh asparagus cochinchinensis, 15 parts of fresh mulberry leaves, and 12 parts of fresh licorice.

[0043] Test Example 1

[0044] 1 Materials

[0045] Experimental group 1: 20 parts of fresh mugwort leaves, 5 parts of fresh asparagus cochinchinensis, 10 parts of fresh mulberry leaves, and 5 parts of fresh licorice.

[0046] Experimental group 2: 20 portions of fresh Panax notoginseng, 5 portions of fresh Asparagus cochinchinensis, 10 portions of fresh mulberry leaves, and 5 portions of fresh licorice.

[0047] Experimental group 3: 20 portions of fresh mugwort leaves and 5 portions of fresh licorice.

[0048] Experimental group 4: 5 portions of fresh asparagus, 10 portions of fresh mulberry leaves, and 5 portions of fresh licorice.

[0049] In each group of the Chinese herbal medicine fresh drug composition, fresh wormwood leaf is the fresh leaf of Artemisia argyi Levl.et Vant. of Asteraceae; fresh asparagus is the fresh root of Asparagus cochinchinensis (Lour.) Merr. of Liliaceae; fresh mulberry leaf is the fresh leaf of Morus alba L. of Moraceae; fresh licorice is the fresh root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of Leguminosae; fresh Panax notoginseng is the fresh root and rhizome of Panax notoginseng (Burk.) FHChen of Araliaceae. The above medicinal materials are freshly picked when used without any processing or preparation.

[0050] Weigh each raw material according to the above weight proportions, add appropriate amount of pure water to squeeze the juice using a wall breaking machine, and filter to obtain a filtrate; freeze and vacuum concentrate the filtrate to 0.5 g / mL to obtain the test 1-4 groups of Chinese medicine stock solutions, and store them in a refrigerator at 0-4°C for later use.

[0051] Dexamethasone, Zhejiang Xianjun Pharmaceutical Co., Ltd., product batch number LB2146.

[0052] Lanqin oral liquid, Yangtze River Pharmaceutical Group Co., Ltd., product batch number 21020682.

[0053] 2 Methods

[0054] 2.1 Cell culture

[0055] RAW264.7 cells were revived in DMEM complete medium (containing 10% fetal bovine serum and 1% anti-penicillin-streptomycin mixture), cultured at 37°C and 5% CO2, the medium was changed every 2-3 days, and the cells were subcultured at a ratio of 1:3.

[0056] 2.2 Cell viability assay

[0057] RAW264.7 cells in logarithmic growth phase were collected and 2×10 5 The cells were inoculated at a concentration of 100 μL / well in a 96-well plate and cultured for 24 hours. A blank control group and test groups 1-4 were set up, with 3 replicates in each group. The normal group was given 200 μL of culture medium, and the test groups 1-4 were given 200 μL of drug-containing culture medium. The culture was continued for 24 hours, the culture medium was replaced, and 180 μL of culture medium and 20 μL of CCK-8 were added to each well. After incubation for 1-4 hours, the absorbance was measured at 450 nm on an ELISA reader to calculate the cell viability. Cell viability = (OD 药物组 -OD 空白组 ) / OD空白组 ×100%.

[0058] 2.3 Detection of cellular inflammatory factors

[0059] RAW264.7 cells in logarithmic growth phase were collected and 2×10 5 The cells were inoculated into a 96-well plate at a concentration of 1 μg / well and cultured for 24 hours. A blank control group and test groups 1-4 were set up, with 3 replicates in each group. The culture medium containing test groups 1-4, dexamethasone, and Lanqin oral solution was added to each well, and the blank control group was given an equal volume of culture medium. After 1 hour, LPS with a final concentration of 1 μg / mL was added to each well for stimulation. After 24 hours of culture, the culture medium was collected and rotated at 3000 r·min -1 The cell culture fluid of each group was centrifuged for 15 min under the same conditions, and the supernatant was collected. The secretion of TNF-α and IL-6 in the supernatant was detected by ELISA.

[0060] 2.4 Statistical methods

[0061] Data analysis was performed using SPSS v.21.0 statistical software. Results are expressed as mean ± standard deviation (s), two independent samples were analyzed using one-way ANOVA.

[0062] 3 Experimental results

[0063] 3.1 Effects of each group of drugs on cell viability

[0064] The effects of each group of drugs on the viability of RAW264.7 cells are shown in Table 1. Compared with the blank control group, there was no significant difference in the cell viability of each drug group (P>0.05), and all were above 95%, indicating that each drug group had good safety.

[0065] Table 1 Effects of each group of drugs on RAW264.7 cell viability

[0066] Grouping Cell viability Blank control group 100.26±0.67 Test Group 1 98.76±0.31 Test Group 2 96.42±3.69 Experiment 3 groups 97.40±2.90 Test 4 groups 96.34±4.30

[0067] 3.2 Effects of each group of drugs on the release of inflammatory factors from RAW264.7 cells

[0068] The effects of each group of drugs on the secretion of TNF-α and IL-6 by RAW264.7 cells induced by LPS are shown in Table 2. Compared with the blank control group, the secretion levels of TNF-α and IL-6 by RAW264.7 cells in the model group were significantly increased (P<0.05), indicating that the inflammatory model was successfully established. Compared with the model control group, each drug group could inhibit the secretion of TNF-α and IL-6 by RAW264.7 cells to varying degrees. Among them, the secretion levels of TNF-α and IL-6 in the experimental group 1 were the lowest, showing the most significant anti-inflammatory effect, which was better than the positive Chinese medicine Lanqin oral liquid group, and the difference was statistically significant (P<0.05). The experimental groups 2, 3 and 4 also showed certain anti-inflammatory effects, but the anti-inflammatory effect of the experimental group 2 was not as significant as that of the experimental group 1 due to the use of fresh Panax notoginseng instead of fresh Artemisia argyi. The anti-inflammatory effect of the experimental group 3 was slightly inferior to that of the experimental group 1 due to the lack of ministerial and adjuvant drugs. The anti-inflammatory effect of the experimental group 4 was relatively weak due to the lack of monarch drugs. The above results show that the various components in the fresh Chinese medicinal composition of the present invention have a synergistic effect and are indispensable, and fresh wormwood leaves play an irreplaceable role in this prescription.

[0069] Table 2 Effects of each group of drugs on the secretion of TNF-α and IL-6 by RAW264.7 cells

[0070]

[0071] Note: Data in the same column with different letters indicate significant differences (P<0.05), and data with the same letters indicate no significant differences (P>0.05).

[0072] Test Example 2

[0073] 1 Experimental Materials

[0074] 6-8 weeks old SD rats, weighing 200-220 g, half male and half female, were provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0075] Artemisia argyi, asparagus cochinchinensis, mulberry leaves and licorice were all purchased from Zhang Zhongjing Pharmacy.

[0076] 2 Experimental methods

[0077] 2.1 Experimental drug preparation

[0078] Experimental group 1: 20 parts of fresh mugwort leaves, 5 parts of fresh asparagus cochinchinensis, 10 parts of fresh mulberry leaves, and 5 parts of fresh licorice.

[0079] Weigh the raw materials according to the above weight proportions, add appropriate amount of pure water to squeeze the juice using a wall breaking machine, and filter to obtain a filtrate; freeze-vacuum concentrate the filtrate to 0.5 g / mL to obtain the Chinese medicine stock solution of test group 1.

[0080] Experimental group 2: 20 parts of Artemisia argyi, 5 parts of Asparagus cochinchinensis, 10 parts of Morus alba, and 5 parts of Licorice.

[0081] The raw materials were weighed according to the above weight proportions, washed, chopped, mixed, extracted with water, filtered, and frozen and vacuum concentrated to 0.5 g / mL to obtain the Chinese medicine stock solution of the second group of experiments.

[0082] In the traditional Chinese medicine fresh medicine composition, fresh wormwood leaf is the fresh leaf of Artemisia argyi Levl.et Vant. of Asteraceae; fresh asparagus is the fresh root of Asparagus cochinchinensis (Lour.) Merr. of Liliaceae; fresh mulberry leaf is the fresh leaf of Morus alba L. of Moraceae; fresh licorice is the fresh root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of Leguminosae. The above medicinal materials are freshly picked when used without any processing or preparation.

[0083] 2.2 Establishment of acute pharyngitis model

[0084] After one week of adaptive feeding, 60 SD rats were randomly divided into a blank control group, a model control group, a dexamethasone group, a Lanqin oral liquid group, and an experimental group 1-2, with 10 rats in each group. A laryngeal sprayer was used to spray 20% ammonia water on the pharynx of the rats, 3 sprays / times (0.2-0.4mL), 2 times / day, for 3 consecutive days; the blank control group was sprayed with the same amount of pure water in the same way. During the modeling period, the rats were free to eat and drink, and the appearance of the rats was observed every day, including the spirit, hair, scratching frequency, diet and water intake, and redness and swelling of the pharyngeal mucosa. If the rats have increased pharyngeal secretions, mucosal congestion, redness and swelling, bright red, frequent scratching of the sides or lower sides of the mouth, severe ulceration and bleeding, coughing and wheezing, increased activity, and increased water intake, the modeling is successful.

[0085] 2.3 Grouping and Dosing

[0086] The rats with successful modeling were given oral administration at a volume of 1 mL / 100 g at 10 am every day, and the blank control group and model control group were given the same volume of pure water once a day for 7 consecutive days. The bedding of each group of rats was changed regularly and the cages were cleaned in time.

[0087] 2.4 Observation of apparent indicators

[0088] During the modeling and drug administration period, the mental state, body weight, food and water intake, hair gloss, scratching frequency, pharyngeal mucosal morphology, redness and swelling area, secretions and coughing were observed in each group of rats. Semi-quantitative integration was performed according to Table 3 based on the apparent indicators.

[0089] Table 3 Semi-quantitative integral table of apparent indexes

[0090]

[0091] 2.5 Serum index detection

[0092] Rats were fasted for 12 h before blood collection. 2 h after the last administration, they were weighed and anesthetized with 2% sodium pentobarbital 45 mg / kg body weight. Blood was collected from the abdominal aorta and placed in a 2-8°C refrigerator for 1-2 h. The blood was centrifuged at 3500 rpm for 15 min to separate the serum. The levels of TNF-α, IL-1β, IL-6, IL-10, PGE2, and IFN-γ in rat serum were detected by ELISA.

[0093] 2.6 Pharyngeal histopathological observation

[0094] The rats were fasted for 12 hours before sampling. 2 hours after the last administration, the rats were weighed and anesthetized with 45 mg / kg body weight of 2% sodium pentobarbital. The throat tissue was taken, the surrounding tissue and muscle were stripped, and the rats were fixed in 4% paraformaldehyde tissue fixative after being rinsed with saline. After 24 hours, the rats were dehydrated, transparent, paraffin-embedded, sliced, and stained with H&E. The pathological morphological changes of the rat throat tissue were observed under a microscope. The scoring table was obtained based on the pathological differences between the groups, as shown in Table 4.

[0095] Table 4 Pharyngeal histopathological scoring table

[0096]

[0097] 2.7 Statistical analysis

[0098] Data analysis was performed using SPSS v.21.0 statistical software. Results are expressed as mean ± standard deviation (s), two independent samples were analyzed by one-way ANOVA. Pathological scores were analyzed by Ridit test.

[0099] 3 Results

[0100] 3.1 Apparent indicators

[0101] The apparent index scores of rats with acute pharyngitis model in each group are shown in Table 5. The hair, diet and water intake of rats in the blank group were normal, and there was no redness and swelling in the pharynx, and no scratching of the pharynx. Compared with the blank group, the pharynx of rats in the model group was obviously red and swollen, with a larger area, a large amount of secretions, rapid coughing and wheezing, messy and dull hair, frequent scratching of the oropharynx, and a significant decrease in food intake. Compared with the model group, the diet and water intake, body weight, mental state and redness and swelling of the pharyngeal mucosa of rats in each drug group were improved to varying degrees. Among them, the water intake of rats in test groups 1 and 2 gradually recovered, the diet gradually increased, the congestion and redness of the pharyngeal mucosa gradually disappeared, the area was reduced, and the scratching of the pharynx and lips decreased (P<0.01), but the apparent index scores of mice in test group 1 were lower than those in the Lanqin oral liquid group and test group 2, indicating that the therapeutic effect of test group 1 on acute pharyngitis was better than that of the positive Chinese medicine Lanqin oral liquid group and test group 2.

[0102] Table 5 Apparent index scores of each group in rats with acute pharyngitis model

[0103]

[0104]

[0105] Note: Compared with the blank control group, ## P<0.01; compared with the model control group, ** P<0.01.

[0106] 3.2 Effects of each group of drugs on serum inflammatory factors in rats

[0107] The effects of each group of drugs on the levels of serum inflammatory factors in rats with acute pharyngitis model are shown in Tables 6 and 7. Compared with the blank control group, the levels of TNF-α, IL-1β, IL-6, IL-10, and PGE2 in the serum of rats in the model group were significantly increased, and the level of IFN-γ was significantly decreased (P<0.01), indicating that the ammonia-induced acute pharyngitis model of rats was successfully prepared. Compared with the model group, the levels of TNF-α, IL-1β, IL-6, IL-10, and PGE2 in the serum of rats in each drug administration group were significantly decreased (P<0.01), and the level of IFN-γ was significantly increased (P<0.01), and the effect of test group 1 was better than that of the positive Chinese medicine Lanqin oral liquid group and test group 2.

[0108] Table 6 Effects of each group of drugs on the levels of TNF-α, IL-1β and IL-6 in rat serum

[0109]

[0110] Note: Compared with the blank control group, # P<0.05, ## P<0.01; compared with the model control group, *P<0.05, ** P<0.01.

[0111] Table 7 Effects of each group of drugs on serum IL-10, PGE2 and IFN-γ in rats

[0112]

[0113]

[0114] Note: Compared with the blank control group, # P<0.05, ## P<0.01; compared with the model control group, * P<0.05, ** P<0.01.

[0115] 3.3 Pathological changes of pharyngeal tissue

[0116] The results of pharyngeal tissue pathology in each group are shown in Table 8. The mucosal epithelium of rats in the blank group was smooth and flat, the submucosal glands were not dilated, and there was no inflammatory cell infiltration. Compared with the blank group, the pharyngeal tissue pathology of rats in the model group was significantly lesioned, the mucosal epithelial tissue was damaged and detached, hyperplasia and thickening, the submucosal blood vessels were significantly congested and dilated, a large number of inflammatory cells infiltrated, vacuoles were formed in the glands, and obvious swelling was observed (P<0.01). Compared with the model group, each drug-treated group could significantly improve the pharyngeal tissue lesions, the mucosal epithelial damage was reduced, the submucosal glands were slightly dilated, the vascular congestion and dilation phenomenon was significantly reduced, and the inflammatory cell infiltration was significantly reduced (P<0.01), and the therapeutic effect of test group 1 was better than that of the Chinese medicine positive drug Lanqin oral liquid group and test group 2.

[0117] Table 8 Effects of each group on the pathological morphology of pharyngeal tissue in rats with acute pharyngitis

[0118] Grouping - + ++ +++ P Blank control group 10 0 0 0 Model control group 0 1 2 7 <![CDATA[ ## ]]> Dexamethasone group 1 5 4 0 <![CDATA[ ** <!-- 8 -->]]> Lanqin oral liquid group 1 4 4 1 <![CDATA[ ** ]]> Test Group 1 1 5 4 0 <![CDATA[ ** ]]> Test Group 2 0 4 4 2 <![CDATA[ ** ]]>

[0119] Note: Compared with the blank control group, ## P<0.01; compared with the model control group, ** P<0.01.

[0120] In summary, the present invention complies with the theory of traditional Chinese medicine, takes syndrome differentiation and treatment as the principle, and utilizes the characteristics of fresh medicine with rich active ingredients, fast efficacy and good quality to screen out the Chinese medicine fresh medicine composition of the present invention. The above experimental results further prove that the Chinese medicine fresh medicine composition of the present invention can significantly improve the water intake, scratching frequency and pharyngeal mucosal congestion and swelling phenomenon of acute pharyngitis model rats, reduce serum TNF-α, IL-1β, IL-6, IL-10, PGE2 levels, increase IFN-γ levels, effectively inhibit the release of inflammatory factors, reduce inflammation levels, improve throat tissue lesions, and the therapeutic effect is better than that of the positive Chinese medicine Lanqin oral liquid. The present invention provides a new idea and treatment method for the treatment of pharyngitis.

[0121] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicinal fresh medicine composition for pharyngitis, characterized in that: The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 10-30 parts of fresh wormwood leaves, 1-15 parts of fresh asparagus cochinchinensis, 3-20 parts of fresh mulberry leaves, and 1-15 parts of fresh liquorice.

2. A Chinese medicinal fresh medicine composition for pharyngitis according to claim 1, characterized in that: The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 15-25 parts of fresh wormwood leaves, 1-10 parts of fresh asparagus cochinchinensis, 5-15 parts of fresh mulberry leaves, and 1-10 parts of fresh liquorice.

3. A Chinese medicinal fresh medicine composition for pharyngitis according to claim 1, characterized in that: The fresh Chinese medicinal composition is composed of the following raw materials in parts by weight: 20 parts of fresh wormwood leaves, 5 parts of fresh asparagus cochinchinensis, 10 parts of fresh mulberry leaves and 5 parts of fresh liquorice.

4. A Chinese medicinal fresh medicine composition for pharyngitis as claimed in any one of claims 1 to 3, characterized in that: In the traditional Chinese medicine fresh medicine composition, fresh wormwood leaf is the fresh leaf of Artemisia argyi Levl.et Vant. of Asteraceae; fresh asparagus is the fresh root of Asparagus cochinchinensis (Lour.) Merr. of Liliaceae; fresh mulberry leaf is the fresh leaf of Morus alba L. of Moraceae; fresh licorice is the fresh root and rhizome of Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. or Glycyrrhiza glabra L. of Leguminosae. The above medicinal materials are freshly picked when used without any processing or preparation.

5. The method for preparing the fresh Chinese medicinal composition for pharyngitis according to any one of claims 1 to 3, characterized in that: The following steps are involved: Weigh fresh moxa leaves, fresh asparagus, fresh mulberry leaves and fresh licorice according to the above-mentioned weight proportions, use a wall breaking machine to add appropriate amount of pure water to squeeze the juice, filter to obtain the filtrate, and freeze the filtrate and concentrate it in a vacuum.

6. The Chinese medicinal fresh drug composition according to any one of claims 1 to 3 is used to prepare a drug for treating pharyngitis, wherein the drug is prepared into any drug form acceptable to the human body by conventional Chinese medicine pharmaceutical methods and with medically acceptable excipients.

Images