Preparation process and operation method of high-channel-number probe and application of high-channel-number probe in biological scene

Abstract

The invention discloses a preparation process and an operation method of a high-channel-number probe and application in a biological scenario, and belongs to the field of biomedicines.The preparation process of the high-channel-number probe comprises the steps that a planar electrode array is designed and prepared, the planar electrode array is fixed to a pre-configured substrate, and a winding layer is formed; the method comprises the steps of preparing at least one winding layer, preparing at least one winding core, placing the winding core on the edge of the winding layer, winding the winding layer on the winding core to form the cylindrical high-channel-number probe, and enabling a gap to exist between the winding core and the winding layer so that the winding core can be separated from the winding layer. The reel core can be pulled away from the winding layer, meanwhile, the reel core can be rapidly replaced conveniently, the high-channel-number probe can be left in an organism for a long time, interference of the probe on a target object can be reduced, and long-term and stable electrophysiological information monitoring is achieved.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a preparation process, an operation method, and an application in biological scenarios of a high-channel-number probe. Background Art

[0002] The realization of cognitive, sensory and motor functions requires the coordinated work of neurons in multiple brain regions. The stable monitoring of neural activity in multiple brain regions over a large spatiotemporal range is of great significance for understanding the operating mechanism of the nervous system and building complex behavioral brain-computer interfaces. After being implanted into neural tissue, high-channel-count probes can detect large-scale single neuron discharge activities with high spatiotemporal resolution and large spatiotemporal scale.

[0003] Existing high-channel-count probes usually require a carrier to assist the probe in being implanted into tissues. However, there are situations where the carrier and the probe cannot be separated or are difficult to separate. The inability to separate will increase the additional damage of the probe to the tissue, and the difficulty in separation will cause the probe to be taken out when the carrier is withdrawn, resulting in implantation failure. The reel core is a carrier that provides a planar electrode array film for winding operations and can assist in the implantation of high-channel-count probes. It itself does not work for monitoring the electrophysiological information of organisms. If it cannot be extracted and separated from the probe in long-term applications, it will remain in the body, which will increase the mechanical mismatch between the probe and the tissue, and cause an inflammatory response. Therefore, the preparation of a detachable, reel-free high-channel-count probe is crucial for long-term and stable signal monitoring. Summary of the invention

[0004] The present invention provides a preparation process, an operation method, and an application in a biological scenario of a high-channel-number probe, so as to solve the defect in the prior art that a carrier cannot be smoothly separated from the high-channel-number probe.

[0005] On the one hand, the present invention provides a preparation process for a high-channel-count probe, comprising: designing a planar electrode array that matches the characteristics of a target part of a target object; preparing the planar electrode array based on a standard micro-nano processing process, and fixing the planar electrode array on a pre-configured substrate to form a winding layer; preparing at least one scroll core, and placing the scroll core on the edge of the winding layer, and winding the winding layer around the scroll core to form a cylindrical high-channel-count probe, wherein there is a gap between the scroll core and the winding layer so that the scroll core can be separated from the winding layer.

[0006] Optionally, the reel core is made of at least one or more materials selected from metal wire, quartz tube, optical fiber, polymer wire, medical stereo electroencephalogram electrode, and medical intracranial pressure measurement device, and has a diameter of 30 μm-1 mm.

[0007] Optionally, the reel core may be provided in a plurality of pieces, each of the reel core may be made of different materials, and the reel cores made of different and / or same materials may be integrated together to form a reel core with a composite structure.

[0008] Optionally, there is a gap between the scroll core and the winding layer, including: coating a soluble polymer on the surface of the scroll core, winding the winding layer around the scroll core, placing it in water, and forming a gap after the polymer dissolves; the water-soluble polymer includes at least polyethylene oxide solution, silk fibroin solution, methacrylic acid-ethyl acrylate copolymer, sucrose solution, and gelatin.

[0009] Optionally, there is a gap between the reel core and the winding layer, including: winding the winding layer around the reel core to form a high-channel-count probe, placing the high-channel-count probe in a preconfigured alkaline solution, and etching the reel core to make the reel core thinner to form the gap.

[0010] Optionally, there is a gap between the scroll core and the winding layer, including: coating the surface of a single scroll core with an organic polymer, then arranging multiple scroll cores together, and winding a planar electrode array to form a high-channel-count probe; immersing the high-channel-count probe in an organic solvent to dissolve the organic polymer, thereby forming a gap between the scroll core and the planar electrode array to facilitate the separation of the scroll core.

[0011] Optionally, there is a gap between the scroll core and the winding layer, including: bonding the scroll core to the connection between the scroll core and the winding layer with a soluble polymer, when the high channel number probe meets water, the soluble polymer dissolves, and a gap is formed between the scroll core and the winding layer to facilitate the separation of the scroll core.

[0012] On the other hand, the present invention provides an operation method of a high-channel-count probe, comprising the following steps: preparing a high-channel-count probe; implanting the high-channel-count probe into tissue; extracting a reel core from a winding layer to obtain a high-channel-count probe without a reel core; and performing signal acquisition through an acquisition device.

[0013] Optionally, the method of pulling out the reel core from the winding layer to obtain a high-channel-count probe without a reel core comprises: arranging a plurality of reel cores together and fixing both ends of the reel cores with glue to form a reel core row; winding the winding layer around the reel core row, and after the winding is completed, removing the glue-fixed portion, and pulling out the reel cores one by one to obtain a high-channel-count probe without a reel core.

[0014] On the other hand, the present invention provides an application of a high-channel-count probe in a biological scenario, wherein the high-channel-count probe can be applied to organisms, which at least include rodents, dogs, and non-human primates.

[0015] The preparation process, operation method, and application of the high-channel-number probe in biological scenarios provided by the present invention can reduce friction by setting a gap between the reel core and the winding layer, thereby realizing the removal of the reel core from the winding layer and facilitating rapid replacement of the reel core. The high-channel-number probe can remain in the body for a long time, thereby reducing the interference of the probe on the target object and realizing long-term and stable monitoring of electrophysiological information. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the present invention or the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0017] Figure 1 It is a schematic flow chart of a process for preparing a high-channel-number probe provided by an embodiment of the present invention;

[0018] Figure 2 is a schematic diagram of a winding layer of a high channel count probe of the present invention;

[0019] Figure 3 Schematic diagram of winding of winding layers and winding core of the present invention;

[0020] Figure 4 It is a schematic diagram of electrophysiological signal collection of the present invention;

[0021] Figure 5 It is a schematic flow chart of a process for preparing a high-channel-number probe provided by another embodiment of the present invention. DETAILED DESCRIPTION

[0022] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the drawings of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0023] As mentioned above, the existing high-channel-count probe carrier and probe cannot be separated or are difficult to separate, which will cause the probe to be taken out when the carrier is withdrawn, resulting in implantation failure. The reel core is a carrier that provides a planar electrode array film for winding operations and can assist in the implantation of high-channel-count probes. It itself does not work for monitoring the electrophysiological information of the organism. If it cannot be extracted in long-term applications and remains in the organism, it will increase the mechanical mismatch between the probe and the tissue and cause an inflammatory response.

[0024] In view of this, the present invention provides a preparation process of a high-channel-count probe, which enables the preparation of a detachable high-channel-count probe without a reel core to achieve long-term and stable signal monitoring. The present invention solves this problem by:

[0025] Please refer to the instruction manual Figure 1 As shown in the figure, the present invention provides a preparation process of a high-channel-number probe, comprising:

[0026] S100, designing a planar electrode array that matches the characteristics of a target part of a target object;

[0027] S200, preparing the planar electrode array based on standard micro-nano processing technology;

[0028] S300, fixing the planar electrode array on a pre-configured substrate to form a winding layer;

[0029] S400, preparing at least one reel core, and placing the reel core at the edge of the winding layer, and winding the winding layer around the reel core to form a cylindrical high-channel-count probe, with a gap between the reel core and the winding layer so that the reel core can be separated from the winding layer.

[0030] The present invention uses the above method to set a gap between the reel core and the winding layer, and reduces friction through the gap, thereby achieving the purpose of extracting the reel core from the winding layer, and facilitating the rapid replacement of the reel core. In addition, the high-channel number probe can remain in the body for a long time, which can reduce the interference of the probe on the target object and the risk of inflammation of the target object, and finally achieve long-term and stable electrophysiological information monitoring.

[0031] In order to further explain the implementation process of the above embodiment, the present invention provides another preferred embodiment. In another preferred embodiment of the present invention, in step S100, the planar electrode array includes a recording site area, a densely arranged conductive trace area, and an interconnection area, such as Figure 2-Figure 3As shown, the recording sites may have different numbers (such as 32 or 64 or 128 or 256 or 1024), multiple distribution densities, multiple axial and lateral spatial distributions (including spacing, positions, etc.). The interconnection area includes multiple designs to be suitable for multiple connection methods with the back-end circuit, including flip-chip soldering, silver glue connection, wire bonding, direct contact, etc.

[0032] In another preferred embodiment of the present invention, in step S200, the planar electrode array of the present invention comprises an electronic material layer, a base layer and a packaging layer, wherein the electronic material layer is made of gold, platinum, copper, aluminum, silver, titanium, chromium, nickel, tantalum, palladium, molybdenum, iridium, titanium nitride, iridium oxide, carbon nanotubes, graphene, and combinations of these and / or other similar elements; the base layer and the packaging layer are made of thin film materials, such as polyimide (PI), polyparaxylene-C, polydimethylsiloxane (PDMS), polystyrene-block poly(ethylene-butylene)-block polystyrene (SEBS), SU-8, polyethylene terephthalate (PET), polyurethane (PU), Ecoflex, polystyrene-butadiene-styrene (SBS) or Teflon. The planar electrode array is made of micro-nano processing technology, such as thin film deposition technology, photolithography technology, plasma etching technology. The prepared planar electrode array has a thickness between 500nm and 200μm.

[0033] In another preferred embodiment of the present invention, in step S400, the high channel count probe includes probes formed by various planar electrode arrays wound around various reel cores. The probe has a detachable structure, including a reel core and a reel with a hollow structure (i.e., a winding layer). The recording sites of the planar electrode array are sites distributed along the axial and lateral directions of the reel on the probe surface after winding, and the recording sites include various distributions, sizes, densities, and numbers. The interconnection area of ​​the planar electrode array is the interconnection part of the probe after winding, which is used for connection with the back-end circuit, and the connection method includes flip-chip soldering, silver glue connection, wire bonding, direct contact, etc.

[0034] Based on the above content, the reel core of the present invention needs to be wrapped in a winding layer to form a high channel count probe, and the reel core wrapped in the winding layer can be a single reel core or a composite of multiple reel cores. When the reel core is a single reel core, it can be directly wrapped in the winding layer. The reel core can include a variety of diameters, lengths, materials and uses. For example: metal wire, quartz tube, optical fiber, polymer wire, medical stereoelectroencephalography electrode (Stereoelectroencephalography, SEEG), medical intracranial pressure measurement device. And the reel core diameter is 30μm-1mm. When the reel core is a composite structure of multiple reels, it is necessary to process the multiple reel cores. At this time, these reel cores can be of different diameters, lengths, materials and uses, and the method of compounding them together is: parallelly attaching multiple metal wires together and spirally winding multiple metal wires together. The metal wire includes at least tungsten wire, nickel titanium wire, nickel chromium wire, and stainless steel wire.

[0035] Based on the above content, after the winding is completed, the winding layer and the reel core of the present invention will inevitably have friction and van der Waals force between the reel core and the winding layer at the physical level. This part of the force prevents the reel core from being pulled out of the winding layer, as shown in the following formula:

[0036] F=μ·F N

[0037] Where μ is the friction coefficient between the scroll core and the planar electrode array film, F N The normal force includes the external pressure on the probe and the van der Waals force. Since the friction coefficient is less than 1, for example, 0.2-0.4, there is no external pressure involved in the process of extracting the reel core, so the van der Waals force plays a major role.

[0038] The formula for calculating the van der Waals force is:

[0039]

[0040] Among them, A H is the Hamaker constant, D is the wire diameter, L is the length of the reel core, and d is the distance between the reel core surface and the inner surface of the planar electrode array film. Therefore, it can be seen from the above formula that the larger and tighter the contact area between the reel core and the winding layer, the greater the interaction force between the two, and the more difficult it is to pull out the reel core; when the winding layer is thinner, such as below 1μm, the capillary force of the liquid between the film and the reel core pulls the winding layer and the reel core to fit together, increasing the van der Waals force between the two and hindering the smooth extraction of the reel core. Therefore, if you want to pull it out smoothly, you need to create a gap between the reel core and the winding layer, for example: 5-20μm. This gap will minimize the above resistance to the greatest extent, thereby driving the reel core to be smoothly pulled out of the winding layer.

[0041] In another preferred embodiment of the present invention, the above-mentioned method of manufacturing the gap may include: when the reel core is a single one, a soluble polymer may be coated on the surface of the reel core, and after the winding layer is wound around the reel core into a roll, it is placed in water, and after the polymer is dissolved, a gap is formed; the water-soluble polymer includes at least polyethylene oxide solution, silk fibroin solution, methacrylic acid-ethyl acrylate copolymer solution, sucrose solution, and gelatin.

[0042] In another preferred embodiment of the present invention, the above-mentioned method of manufacturing the gap may also include: placing a single or multiple composite scroll cores in a pre-configured alkaline solution (for example: 1 mol / L), the scroll core and the stone mill rod electrode form a dual-electrode system, using constant voltage conditions, the scroll core is the working electrode, the stone mill rod is the counter electrode, and a constant voltage is applied using an electrochemical workstation to achieve electrochemical etching of the scroll core. The etching time can be 180 seconds, so that the diameter of the scroll core becomes thinner, thereby forming a gap to facilitate the separation of the scroll core.

[0043] In another preferred embodiment of the present invention, the above-mentioned method of manufacturing the gap may also include: coating the surface of a single reel core with an organic polymer, such as a positive photoresist, with a thickness of 5-10 μm, such as 5 μm, and then arranging multiple reel cores together and winding the winding layer to form a high-channel number probe, which is immersed in an organic solvent, such as acetone or isopropyl alcohol, to dissolve the photoresist, thereby forming a gap.

[0044] Therefore, by forming the gap in the above manner, the friction between the reel core and the winding layer can be reduced, so that the reel core can be smoothly pulled out without affecting the operation of the high-channel-count probe itself. When the reel core is a single core, the pulling-out method can be direct pulling out. When the reel core is a composite structure of multiple cores, there is also a gap between the reel cores. The interaction force between the two is expressed by the following formula:

[0045]

[0046] Among them, A H is the Hamaker constant, R is the radius of the reel core, and D is the spacing between the reel cores.

[0047] Therefore, in this case, it is necessary to arrange multiple reel cores together, fix their two ends with glue, and wind the fixed reel core row and winding layer together. Then, remove the fixing points at both ends. At this time, there are gaps between the multiple reel cores, and the reel cores have lost the adhesion of glue. Therefore, the reel cores can be taken out of the winding layer one by one to obtain a probe without a reel core.

[0048] In another preferred embodiment of the present invention, another preferred method for removing the reel core is provided, specifically: there is a common connection object between the reel core and the winding layer, that is, the probe is permanently fixed on the circuit board through the interconnection area, and the reel core can be temporarily fixed on the circuit board. At this time, by moving the circuit board, the reel core and the high-channel-number probe are implanted into the target part of the target object at a controllable speed, such as 2μm / s, 10μm / s, 100μm / s. After the probe is implanted, the temporary fixed part of the reel core can be removed, such as the temporary fixing method is to fix the end of the reel core on the circuit board with a soluble polymer. After contacting water, the polymer dissolves to achieve the separation of the reel core and the circuit board, and the reel core is removed from the high-channel-number probe to achieve smooth separation of the structure. The circuit board includes a flexible flat cable and a flexible circuit board. The soluble polymer includes a polyethylene oxide solution, a silk protein solution, a methacrylic acid-ethyl acrylate copolymer solution, a sucrose solution, and a gelatin.

[0049] Accordingly, the preparation process of the high-channel-count probe described in the present invention achieves the separation of the reel core from the winding layer by setting a gap between the reel core and the winding layer, thereby reducing the friction through the gap, and facilitating the rapid replacement of the reel core. The high-channel-count probe can remain in the body for a long time, thereby reducing the interference of the probe on the target object and realizing long-term and stable monitoring of electrophysiological information.

[0050] Based on the same general inventive concept, the present invention also protects a method for operating a high-channel-number probe, which comprises the following steps:

[0051] S1, preparing the above-mentioned high channel number probe;

[0052] S2, implanting high channel count probes into tissues;

[0053] S3, extracting the reel core from the winding layer to obtain a high channel count probe without a reel core;

[0054] S4. Collect signals through acquisition equipment;

[0055] For example, in an embodiment of the present invention, the above steps may be:

[0056] Step 1: Connect the interconnection area of ​​the high-channel-count probe to the back-end circuit to achieve signal acquisition. The interconnection method includes flip-chip soldering, silver glue connection, wire bonding, direct contact, etc. The back-end circuit includes a printed circuit board, a flexible flat cable, and a flexible circuit board.

[0057] Step 2: Fix the biological sample on a stereotaxic instrument.

[0058] Step 3: fix the high-channel-count probe on the clamping arm. The high-channel-count probe may include the reel core before being implanted into the biological body, or the reel core may be pulled out before being implanted into the biological body without including the reel core.

[0059] Step 4, determine the implantation path, implant the high-channel-count probe without a scroll core and retain it in the organism, the implantation method includes opening a bone window, removing the dura mater, directly implanting the high-channel-count probe without a scroll core into the brain tissue, or implanting the high-channel-count probe with a scroll core into the brain tissue and then extracting the scroll core to obtain the high-channel-count probe without a scroll core. Open a bone window, remove the dura mater, directly implant the high-channel-count probe without a scroll core into the spinal cord tissue, or implant the high-channel-count probe with a scroll core into the spinal cord tissue and then extracting the scroll core to obtain the high-channel-count probe without a scroll core. The extraction method includes using a single clamping arm method, extracting the scroll core after the high-channel-count probe is completely implanted; using a double clamping arm method, slowly extracting the scroll core while the high-channel-count probe is implanted, and completely extracting the scroll core after the high-channel-count probe is completely implanted.

[0060] Step 5: Perform subsequent signal acquisition and other tasks through acquisition equipment.

[0061] Based on the same general inventive concept, the present invention also protects the application of a high-channel-count probe in a biological scenario. Specifically, the high-channel-count probe described in the present invention can be applied to organisms including rodents (such as rats), dogs (such as beagles), non-human primates (such as macaques), etc.

[0062] For example, the high-channel-count probe of the present invention is prepared by a micro-nano processing technology to obtain a planar electrode array, the planar electrode array has 128 recording sites, and a 50μm tungsten wire is surface-treated, and then the planar electrode array film is rolled up with a 50μm diameter tungsten wire, and then the tungsten wire is pulled out after winding to obtain a high-channel-count probe without a reel core, and the interconnection area of ​​the high-channel-count probe is connected to the printed circuit board through silver glue, and then the probe is implanted into the neural tissue of the rat at an implantation speed of 2μm / s to achieve the recording of the rat cortical signal by the probe, and the recording results are as follows: Figure 4 As shown in the figure, the probe without a scroll core can stably monitor neural activity in long-term recording, and multiple continuous channels detect the spike activity of the same neuron, indicating that the high-channel number probe described in the present invention has the advantages of high biocompatibility and long-term stability.

[0063] In summary, the preparation process, operation method and application in biological scenarios of the high-channel-number probe described in the present invention have the following advantages:

[0064] (1) High-channel-count probes have high channel counts and small diameters: High-channel-count probes are wound from a planar electrode array film. Dense metal traces are arranged on the planar electrode array, and the film is wrapped layer by layer to achieve the preparation of probes with high channel counts and small diameters.

[0065] (2) The reel core is removable: The reel core can temporarily enhance the mechanical strength of the high-channel-count probe to assist in implanting the probe into the target site. The reel core can be removed from the probe to achieve smooth separation of the structure. This detachable structural design allows for a higher mechanical match between the probe and the target object. At the same time, reel cores of different materials, functions, and lengths can be replaced, thereby achieving multifunctional integration.

[0066] (3) Multifunctionality: The reel core used for winding operation can be made of metal wire, polymer wire, optical fiber, medical stereo EEG electrode, medical intracranial pressure measurement device, etc., so as to realize the synchronous operation and multifunctional integration of electrical measurement, electrical stimulation and other measurements. In the same experiment, since the reel core is removable, multiple functional reel cores can be replaced to achieve different functions.

[0067] (4) Controllable implantation speed: The reel core can assist the high-channel-count probe in being implanted into the target part of the target object at different speeds, such as 2 μm / s, 10 μm / s, and 100 μm / s. The two have a common connection object. After the high-channel-count probe is implanted, the temporary fixed part of the reel core on the connection object can be removed, and the reel core is removed from the high-channel-count probe to achieve smooth separation of the structure.

[0068] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A process for preparing a high-channel-number probe, characterized in that: include: Designing a planar electrode array that matches the characteristics of the target site of the target subject; The planar electrode array is prepared based on standard micro-nano processing technology; Fixing the planar electrode array on a pre-configured substrate to form a winding layer; At least one winding core is prepared and placed on the edge of the winding layer, and the winding layer is wound around the winding core to form a cylindrical high channel number probe, with a gap between the winding core and the winding layer so that the winding core can be separated from the winding layer.

2. The preparation process according to claim 1, characterized in that: The reel core is made of at least one or more materials selected from metal wire, quartz tube, optical fiber, polymer wire, medical stereo electroencephalogram electrode, and medical intracranial pressure measuring device, and has a diameter of 30 μm-1 mm.

3. The preparation process according to claim 1, characterized in that: The reel core may be provided in a plurality of pieces, each of the reel core may be made of different materials, and the reel cores made of different and / or same materials may be integrated together to form a reel core with a composite structure.

4. The preparation process according to claim 1, characterized in that: There is a gap between the reel core and the winding layer, including: A soluble polymer is coated on the surface of the reel core, and after the winding layer is wound around the reel core into a roll, the reel core is placed in water, and a gap is formed after the soluble polymer is dissolved; The soluble polymer at least includes polyethylene oxide solution, silk fibroin solution, methacrylic acid-ethyl acrylate copolymer solution, sucrose solution and gelatin.

5. The preparation process according to claim 3, characterized in that: There is a gap between the reel core and the winding layer, which includes: winding the winding layer around the reel core to form a high channel number probe, placing the high channel number probe in a pre-configured alkaline solution, and etching the reel core to make the reel core thinner to form the gap.

6. The preparation process according to claim 3, characterized in that: There is a gap between the scroll core and the winding layer, including: coating the surface of a single scroll core with an organic polymer, then arranging multiple scroll cores together, and winding a planar electrode array to form a high-channel-number probe; immersing the high-channel-number probe in an organic solvent to dissolve the organic polymer, thereby forming a gap between the scroll core and the planar electrode array to facilitate the separation of the scroll core.

7. The preparation process according to claim 3, characterized in that: There is a gap between the scroll core and the winding layer, which includes: bonding the scroll core with a soluble polymer at the connection between the scroll core and the winding layer, when the high channel number probe meets water, the soluble polymer dissolves, and a gap is formed between the scroll core and the winding layer to facilitate the separation of the scroll core.

8. A method for operating a high-channel-number probe, characterized in that: The steps include: Prepare the high channel number probe as described in any one of claims 1 to 7; implanting high-channel-count probes into tissue; The reel core is pulled out from the winding layer to obtain a high channel count probe without a reel core; Signal collection is performed through acquisition equipment.

9. The operating method according to claim 8, characterized in that: The method of extracting the reel core from the winding layer to obtain a high channel count probe without a reel core comprises: Arrange several scroll cores together and fix the two ends of the scroll cores with glue to form a scroll core row; The winding layer is wound around the reel core row, and after the winding is completed, the glue-fixed portion is removed, and the reel cores are pulled out one by one to obtain a high-channel-count probe without a reel core.

10. An application of a high-channel-number probe in a biological scenario, characterized in that: The high channel number probe can be applied to organisms, which at least include rodents, dogs, and non-human primates.

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