Portable liquid biopsy kit for rapid detection of lung cancer glycosylation marker

Abstract

The present invention relates to the technical field of liquid biopsy kits, and discloses a lung cancer glycosylation marker rapid detection portable liquid biopsy kit, which comprises a kit body, the kit body is internally provided with an accommodating mechanism, one side of the accommodating mechanism is provided with a pressing mechanism, the bottom of the accommodating mechanism is provided with a transmission mechanism, and the transmission mechanism is connected with the pressing mechanism. A cooling mechanism is arranged on one side of the kit body, and a sealing mechanism is arranged at the top of the kit body; the containing mechanism comprises a containing plate, the exterior of the containing plate is fixedly connected to the inner wall of the kit body, and a plurality of containing grooves are formed in the top of the containing plate. The test tube is placed in the containing groove and pressed downwards, so that the test tube extrudes the limiting clamping block to retract, the test tube continues to move downwards, the limiting clamping block is matched with the rebound effect of the compression spring, and the test tube enters the limiting clamping groove, in this way, the test tube can be fixed and unlocked more conveniently, time is saved, and operation convenience is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of liquid biopsy kits, and in particular to a portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers. Background Art

[0002] With the continuous development of early cancer screening and diagnosis technology, liquid biopsy technology has become a non-invasive tumor detection method and is widely used in cancer marker detection. Liquid biopsy mainly collects blood, urine or other body fluid samples from patients. Portable liquid biopsy kits can be quickly implemented at clinical sites or at patients' homes, which is convenient for medical staff or patients to use directly. The test results are fast and therefore widely used.

[0003] However, the test tube fixing method of existing portable liquid biopsy kits is usually relatively simple, such as using a single card slot or plastic clamp. However, during the detection process, it is easy for external factors to cause vibration or operation to cause the test tube to shake, thereby affecting the sample in the test tube, and then affecting the accuracy of the test results. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers, which solves the problem that the test tube fixing method of the existing portable liquid biopsy kit is usually relatively simple, such as using a single card slot or plastic clamp, but during the detection process, it is easy to cause vibration due to external factors or shaking of the test tube due to operation, thereby affecting the sample in the test tube, and then affecting the accuracy of the test results.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers, comprising a kit body, a containing mechanism is arranged inside the kit body, a pressing mechanism is arranged on one side of the containing mechanism, a transmission mechanism is arranged at the bottom of the containing mechanism, a cooling mechanism is arranged on one side of the kit body, and a sealing mechanism is arranged on the top of the kit body;

[0006] The accommodating mechanism includes a accommodating plate, the outside of which is fixedly connected to the inner wall of the reagent box body, a plurality of accommodating grooves are provided on the top of the accommodating plate, test tubes are arranged inside the accommodating grooves, a plurality of limiting slots are provided on the bottom of the test tubes, the inner wall of the accommodating plate is slidably connected to a limiting block, a compression spring is installed inside the accommodating plate, one end of the compression spring is fixedly connected to one end of the limiting block, and the other end of the compression spring is fixedly connected to the inner wall of the accommodating plate.

[0007] Preferably, the pressing mechanism includes a plurality of limit sliders, the outer portion of the limit sliders is slidably connected to the inner wall of the accommodating plate, the inner wall of the limit slider is fixedly connected to a pressing rod, an extrusion spring is installed inside the accommodating plate, and the top of the limit block is fixedly connected to a connecting block.

[0008] Preferably, the transmission mechanism includes a connecting slider, both ends of which are slidably connected to the inner wall of the accommodating plate, connecting grooves are provided on both sides of the accommodating groove, and a connecting rope is provided inside the connecting groove, one end of the connecting rope is fixedly connected to one side of the connecting slider, and the other end of the connecting rope is fixedly connected to the bottom of the positioning block.

[0009] Preferably, the cooling mechanism includes a fixed block, one side of the fixed block is fixedly connected to the outside of the test kit body, a cooler is installed inside the fixed block, the input end of the cooler is fixedly connected to an air inlet pipe, the other end of the cooler is fixedly connected to an air outlet pipe, a disassembly block is installed outside the air inlet pipe, and a filter is detachably installed on the inner wall of the disassembly block.

[0010] Preferably, a sealing cover is rotatably connected to the top of the reagent box body, and a baffle is fixedly connected to the top of the containing plate.

[0011] Preferably, the sealing mechanism comprises a sealing block, the inner wall of which is fixedly connected to the top of the reagent box body, the inner wall of the sealing cover is provided with a sealing groove, and the outer portion of the sealing block is in contact with the sealing groove.

[0012] Preferably, a groove is provided on the top of the containing plate, a reagent card is arranged in the groove, the outside of the positioning block is slidably connected to the inner wall of the containing plate, and one end of the positioning block is in contact with the outside of the test tube.

[0013] Preferably, the pressing rod is arranged directly above the connecting block, and one end of the connecting block is arranged as an inclined surface.

[0014] Preferably, a longitudinal section of one end of the limit card block is arranged to be square, and one end of the limit card block is plug-fitted with the limit card slot.

[0015] Working principle: when using the device, the sealing cover is opened to place the test tube inside the receiving groove on the top of the receiving plate, and the test tube is pressed downward so that the bottom of the test tube squeezes one end of the limit card block, thereby squeezing the compression spring to retract the limit card block, so that when the test tube moves downward to a certain position, the compression spring can be used to push one end of the limit card block into the interior of the limit card groove, thereby conveniently fixing the bottom of the test tube, and the pressing rod can be pressed downward to squeeze the squeezing spring, so that one end of the pressing rod moves downward, and then one end of the pressing rod squeezes the connecting block, thereby driving the limit card block to retract, so that the test tube can be pulled upward to conveniently take out the test tube;

[0016] During the downward movement of the test tube, the connecting slider is squeezed to move downward synchronously, thereby driving the connecting ropes on both sides to move inside the connecting groove. Under the traction force of the connecting rope, one end of the connecting rope pulls the positioning block to move on the inner wall of the containing plate, so that one end of the positioning block clamps the outside of the test tube, thereby effectively preventing the test tube from shaking.

[0017] By starting the cooler and cooperating with the function of the air intake pipe, the external gas is extracted and cooled by the cooler. The dust can be effectively filtered with the function of the filter to prevent dust from entering the machine and affecting the operation. The cooled gas is then transported to the inside of the test kit body through the air outlet pipe, thereby cooling the inside of the test kit body. In this way, the sealing cover and the test kit body are closed, and the inside of the test kit body is sealed by the mutual cooperation of the sealing block and the sealing groove.

[0018] The present invention provides a portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers. It has the following beneficial effects:

[0019] 1. The present invention places the test tube in the accommodating groove and presses the test tube downward, so that the test tube squeezes the limit card block to retract, so that when the test tube continues to move downward, the limit card block cooperates with the rebound effect of the compression spring to enter the interior of the limit card groove, and by pressing the pressing rod downward, one end of the pressing rod squeezes the connecting block to drive the limit card block to retract, so that the test tube can be conveniently taken out, so that the fixing and unlocking operations of the test tube can be more convenient, and the test tube can be conveniently taken out, thereby saving time and improving operation convenience.

[0020] 2. The present invention moves the connecting slider synchronously by moving the test tube downward, thereby driving the connecting ropes on both sides to pull the positioning block to move on the inner wall of the containing plate, so that one end of the positioning block can clamp the outside of the test tube, thereby effectively preventing the test tube from shaking. In this way, while the test tube is fixed, it can also prevent it from shaking during storage, thereby reducing the impact of the external environment on the test tube sample.

[0021] 3. The present invention starts the cooler, draws external gas into the cooler through the air inlet pipe for cooling, and cooperates with the filter to effectively filter dust. The cooled gas is then transported to the inside of the reagent box through the air outlet pipe for cooling. In this way, by transporting the cooled gas to the inside of the reagent box, a stable low-temperature environment is provided during the sample storage process to prevent temperature fluctuations from affecting the sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 A perspective view of the present invention;

[0023] Figure 2 It is a schematic diagram of the structure of the air intake pipeline of the present invention;

[0024] Figure 3 It is a schematic diagram of the sealing block structure of the present invention;

[0025] Figure 4 It is a schematic diagram of the structure of the accommodation plate of the present invention;

[0026] Figure 5 It is a schematic diagram of the limit block structure of the present invention;

[0027] Figure 6 for Figure 5 Enlarged view of point A in the middle;

[0028] Figure 7 It is a schematic diagram of the filter structure of the present invention.

[0029] Among them, 1. reagent box body; 2. sealing cover; 3. containing mechanism; 301. containing plate; 302. containing groove; 303. test tube; 304. compression spring; 305. limit block; 306. limit groove; 4. pressing mechanism; 401. pressing rod; 402. limit slider; 403. extrusion spring; 404. connecting block; 5. transmission mechanism; 501. connecting slider; 502. connecting rope; 503. connecting groove; 504. positioning block; 6. reagent card; 7. cooling mechanism; 701. cooler; 702. air inlet pipe; 703. filter net; 704. air outlet pipe; 705. fixing block; 706. disassembly block; 8. baffle; 9. sealing mechanism; 901. sealing block; 902. sealing groove. DETAILED DESCRIPTION

[0030] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] Please see attached Figure 2 and attached Figure 5 The embodiment of the present invention provides a portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers, including a kit body 1, wherein a containing mechanism 3 is arranged inside the kit body 1, wherein the containing mechanism 3 is used to fix a test tube 303 and to contain the test tube 303, a pressing mechanism 4 is arranged on one side of the containing mechanism 3, wherein the pressing mechanism 4 is used to conveniently take out the test tube 303, a transmission mechanism 5 is arranged at the bottom of the containing mechanism 3, wherein the transmission mechanism 5 is used for the test tube 303 to cooperate with the containing mechanism 3 in the fixing process to move in coordination, a cooling mechanism 7 is arranged on one side of the kit body 1, wherein the cooling mechanism 7 is used to cool the inside of the kit body 1 to ensure that the sample and the reagent are stored at a suitable temperature, and a sealing mechanism 9 is arranged on the top of the kit body 1, wherein the sealing mechanism 9 is used to ensure the sealing of the kit body 1.

[0032] Please see attached Figure 4 and attached Figure 6The containing mechanism 3 includes a containing plate 301, the outside of which is fixedly connected to the inner wall of the reagent box body 1, a plurality of containing grooves 302 are provided on the top of the containing plate 301, a test tube 303 is arranged inside the containing groove 302, wherein the containing groove 302 is used to provide a stable foundation for the placement of the test tube 303, a plurality of limiting grooves 306 are provided at the bottom of the test tube 303, wherein the test tube 303 is used as a container for placing the sample, the inner wall of the containing plate 301 is slidably connected to a limiting block 305, a compression spring 304 is installed inside the containing plate 301, one end of the compression spring 304 is fixedly connected to one end of the limiting block 305, and the other end of the compression spring 304 is fixedly connected to the inner wall of the containing plate 301. The function of the compression spring 304 is to provide a rebound force after the test tube 303 is removed, driving the limit block 305 to reset. A groove is provided at the top of the receiving plate 301, and a reagent card 6 is arranged in the groove, wherein the reagent card 6 is used to carry the living body reagent to be detected. The outside of the positioning block 504 is slidably connected to the inner wall of the receiving plate 301, and one end of the positioning block 504 is in contact with the outside of the test tube 303. The positioning block 504 is used to further stabilize the outside of the test tube 303 to prevent the test tube 303 from shaking or deflecting due to external factors after insertion. The top of the reagent box body 1 is rotatably connected to the sealing cover 2, and the top of the receiving plate 301 is fixedly connected to the baffle 8. The sealing cover 2 is used to protect the instrument after the test tube 303 is placed. The longitudinal section of one end of the limit block 305 is set to be square. One end of the limit block 305 is plug-in matched with the limit slot 306. The limit block 305 cooperates with the limit slot 306 to fix the test tube 303 through plug-in.

[0033] Please see attached Figure 5 and attached Figure 6The pressing mechanism 4 includes a plurality of limit sliders 402, the outer portion of the limit slider 402 is slidably connected to the inner wall of the accommodating plate 301, the inner wall of the limit slider 402 is fixedly connected with a pressing rod 401, ensuring that pressing the pressing rod 401 enables the limit slider 402 to move stably, an extrusion spring 403 is installed inside the accommodating plate 301, and a connecting block 404 is fixedly connected to the top of the limit block 305. The transmission mechanism 5 includes a connecting slider 501, wherein the extrusion spring 403 is used to provide a reaction force after the pressing action is completed, so that the limit slider 402 and the pressing rod 401 are reset, the two ends of the connecting slider 501 are slidably connected to the inner wall of the receiving plate 301, and the two sides of the receiving groove 302 are provided with a connecting groove 503, wherein the limit block 305 is driven to retract through the connecting block 404 to release the fixation of the test tube 303, and the connecting groove 503 is provided inside, and one end of the connecting rope 502 is fixedly connected to one side of the connecting slider 501, and the other end of the connecting rope 502 is fixedly connected to the bottom of the positioning block 504. The connecting groove 503 cooperates with the connecting rope 502 to smoothly drive the connecting slider 501 to move, the pressing rod 401 is arranged just above the connecting block 404, and one end of the connecting block 404 is arranged as an inclined surface.

[0034] Please see attached Figure 1 , Attachment Figure 3 and attached Figure 7 The cooling mechanism 7 includes a fixed block 705, one side of which is fixedly connected to the outside of the reagent box body 1, a cooler 701 is installed inside the fixed block 705, an input end of the cooler 701 is fixedly connected to an air inlet pipe 702, wherein the cooler 701 is used to cool the temperature of the gas entering the reagent box body 1, and the other end of the cooler 701 is fixedly connected to an air outlet pipe 704, a disassembly block 706 is installed outside the air inlet pipe 702, wherein the air inlet pipe 702 and the air outlet pipe 704 are used to transport the cooling gas to the inside of the reagent box body 1, and a filter screen 703 is detachably installed on the inner wall of the disassembly block 706. The filter screen 703 is used to prevent dust and other impurities from entering the reagent box body 1. The sealing mechanism 9 includes a sealing block 901. The inner wall of the sealing block 901 is fixedly connected to the top of the reagent box body 1. The inner wall of the sealing cover 2 is provided with a sealing groove 902. The outer part of the sealing block 901 is fitted with the sealing groove 902. The mutual cooperation between the sealing groove 902 and the sealing block 901 ensures the sealing of the reagent box body 1 and prevents the entry of external air or pollutants.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers, comprising a kit body (1), characterized in that: A containing mechanism (3) is arranged inside the reagent box body (1), a pressing mechanism (4) is arranged on one side of the containing mechanism (3), a transmission mechanism (5) is arranged at the bottom of the containing mechanism (3), a cooling mechanism (7) is arranged on one side of the reagent box body (1), and a sealing mechanism (9) is arranged on the top of the reagent box body (1); The accommodating mechanism (3) comprises an accommodating plate (301), the outside of the accommodating plate (301) is fixedly connected to the inner wall of the reagent box body (1), a plurality of accommodating grooves (302) are provided on the top of the accommodating plate (301), a test tube (303) is arranged inside the accommodating groove (302), a plurality of limiting clamping grooves (306) are provided at the bottom of the test tube (303), the inner wall of the accommodating plate (301) is slidably connected to a limiting clamping block (305), a compression spring (304) is installed inside the accommodating plate (301), one end of the compression spring (304) is fixedly connected to one end of the limiting clamping block (305), and the other end of the compression spring (304) is fixedly connected to the inner wall of the accommodating plate (301).

2. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 1, characterized in that: The pressing mechanism (4) comprises a plurality of limit sliders (402), the outer portions of the limit sliders (402) being slidably connected to the inner wall of the receiving plate (301), the inner wall of the limit sliders (402) being fixedly connected to a pressing rod (401), the interior of the receiving plate (301) being provided with a pressing spring (403), and the top of the limit block (305) being fixedly connected to a connecting block (404).

3. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 1, characterized in that: The transmission mechanism (5) comprises a connecting slider (501), both ends of the connecting slider (501) are slidably connected to the inner wall of the accommodating plate (301), both sides of the accommodating groove (302) are provided with connecting grooves (503), a connecting rope (502) is arranged inside the connecting groove (503), one end of the connecting rope (502) is fixedly connected to one side of the connecting slider (501), and the other end of the connecting rope (502) is fixedly connected to the bottom of the positioning block (504).

4. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 1, characterized in that: The cooling mechanism (7) comprises a fixed block (705), one side of which is fixedly connected to the outside of the reagent box body (1), a cooler (701) is installed inside the fixed block (705), an input end of the cooler (701) is fixedly connected to an air inlet pipe (702), the other end of the cooler (701) is fixedly connected to an air outlet pipe (704), a disassembly block (706) is installed outside the air inlet pipe (702), and a filter screen (703) is detachably installed on the inner wall of the disassembly block (706).

5. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 1, characterized in that: The top of the reagent box body (1) is rotatably connected to a sealing cover (2), and the top of the containing plate (301) is fixedly connected to a baffle (8).

6. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 5, characterized in that: The sealing mechanism (9) comprises a sealing block (901), the inner wall of which is fixedly connected to the top of the reagent box body (1), the inner wall of the sealing cover (2) is provided with a sealing groove (902), and the outside of the sealing block (901) is in contact with the sealing groove (902).

7. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 3, characterized in that: A groove is provided at the top of the containing plate (301), a reagent card (6) is arranged in the groove, the outside of the positioning block (504) is slidably connected to the inner wall of the containing plate (301), and one end of the positioning block (504) is in contact with the outside of the test tube (303).

8. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 2, characterized in that: The pressing rod (401) is arranged directly above the connecting block (404), and one end of the connecting block (404) is arranged as an inclined surface.

9. The portable liquid biopsy kit for rapid detection of lung cancer glycosylation markers according to claim 1, characterized in that: A longitudinal section of one end of the limit card block (305) is arranged to be square, and one end of the limit card block (305) is plug-fitted into the limit card slot (306).

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