Extraction method of xanthoceras sorbifolia bunge oil

Through the 60Co-γ ray irradiation and frying treatment of Wenguan fruit oil, combined with the spiral oil press at low temperature, the problems of grease color deepening, nutrient loss and environmental pollution in the existing technology are solved, and efficient, environmentally friendly and safe Wenguan fruit oil extraction effect is achieved.

CN119875734APending Publication Date: 2025-04-25长春科技学院
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Patent Information

Application Number
CN202510075778.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-17
Publication Date
2025-04-25

AI Technical Summary

Technical Problem

The existing Wenguan fruit oil extraction method has problems such as deepening oil color, loss of nutrients, and environmental pollution, and the traditional solvent extraction method has problems such as solvent residues and safety hazards.

Method used

The raw Wenguan fruit is treated with 60Co-γ ray irradiation, combined with the fried mature Wenguan fruit, the kernel-shell ratio is controlled to be 8.5:1.5, and pressed at low temperature through a spiral oil press to avoid the use of harmful solvents, and achieve residual and pollution-free oil extraction.

Benefits of technology

Maximize the nutritional ingredients in Wenguan Fruit Oil, improve the quality of oil, reduce environmental pollution, reduce production costs, improve resource utilization, and ensure product safety and high quality.

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Abstract

The invention provides an extraction method of xanthoceras sorbifolia bunge oil, and belongs to the field of xanthoceras sorbifolia bunge oil extraction methods. The method comprises the following steps: performing < 60 > Co-gamma ray irradiation treatment on raw xanthoceras sorbifolia bunge to obtain irradiated raw xanthoceras sorbifolia bunge, stir-frying cooked xanthoceras sorbifolia bunge to obtain stir-fried cooked xanthoceras sorbifolia bunge, and mixing the irradiated raw xanthoceras sorbifolia bunge and the stir-fried cooked xanthoceras sorbifolia bunge to obtain a mixed raw material; crushing the mixed raw materials, and crushing the seed kernels to be 24-40 meshes in particle size; mixing kernels and shells in the crushed shiny-leaved yellowhorn, and controlling the kernel-shell ratio in the mixing ratio to be 8.5: 1.5; and squeezing the mixed material, filtering and separating to obtain the shiny-leaved yellowhorn oil. According to the extraction method, nutritional ingredients in the xanthoceras sorbifolia bunge oil are reserved to the maximum extent, the oil quality is improved, and meanwhile environmental pollution is reduced.
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Description

Technical Field

[0001] The invention belongs to the field of Xanthoceras sorbifolia oil extraction methods and specifically provides a method for extracting Xanthoceras sorbifolia oil. Background Art

[0002] With the increasing demand for healthy edible oil and the in-depth implementation of the concepts of environmental protection and sustainable development, the production process of vegetable oil has received more and more attention. Among the many sources of vegetable oil, Xanthoceras sorbifolia has gradually emerged with its unique advantages. Xanthoceras sorbifolia oil is a precious medicinal and edible oil with high nutritional value. It is mainly derived from the seeds and kernels of Xanthoceras sorbifolia, especially the kernels, which contain 50%-70% oil. The extraction of Xanthoceras sorbifolia oil is mostly carried out by cold pressing, hot pressing, solvent extraction, supercritical CO2 extraction, Soxhlet extraction, water enzyme method, etc.

[0003] The article numbered DOI:10.13496 / j.issn.1007-5119.2021.03.013 points out that although the hot pressing method has a slightly higher oil yield, its disadvantage is that it will cause the color of the oil to darken, damage the fat-soluble vitamins in the oil, and produce harmful substances such as oil polymers and trans fatty acids, which are not conducive to the further use of the oil. The content of plant sterols, squalene and vitamins in cold-pressed oil is significantly higher than that in hot-pressed oil. The comparison of component analysis shows that cold-pressed oil has obvious advantages over hot-pressed oil in these aspects. The supercritical CO2 extraction method has a high extraction rate but the equipment is expensive and the industrial production cost is high. The enzymatic hydrolysis time of the aqueous enzyme method is too long and the operation is complicated, which is not conducive to industrial production.

[0004] Although the extraction rate of the solvent extraction method is relatively high, the solvent residue is difficult to completely remove, which not only affects the flavor of the oil, but also endangers health if consumed for a long time. It is even more limited in the field of high-end edible oils and medicinal oils. Most of the commonly used solvents are flammable and explosive. During the extraction operation, they are prone to explosion and fire accidents, which seriously threaten the safety of personnel; and the volatile solvents produce toxic gases. In terms of environmental protection, the extraction process will produce a large amount of wastewater, which is mixed with solvents and impurities, and it is necessary to pay attention to the handling. In addition, this method requires high temperature and high pressure conditions. During this process, the nutrients in the oil are easily lost, such as vitamin E, and even harmful trans fatty acids and other substances may be produced, which reduces the nutritional value of the oil. Summary of the invention

[0005] The purpose of the present invention is to provide a method for extracting Xanthoceras sorbifolia oil, which can retain the nutritional components in the Xanthoceras sorbifolia oil to the maximum extent, improve the quality of the oil, and reduce environmental pollution.

[0006] In order to achieve the above object, the present invention adopts the following technical solution:

[0007] The present invention provides a method for extracting Xanthoceras sorbifolia oil, comprising:

[0008] Step 1: Process the raw Xanthoceras sorbifolia 60 irradiating the fruit with Co-γ rays to obtain irradiated raw Xanthoceras sorbifolia, frying the cooked Xanthoceras sorbifolia to obtain fried cooked Xanthoceras sorbifolia, and mixing the irradiated raw Xanthoceras sorbifolia and the fried cooked Xanthoceras sorbifolia to obtain a mixed raw material;

[0009] Step 2: crushing the mixed raw materials in step 1;

[0010] Step 3: Mix the kernels and shells of the Xanthoceras sorbifolia crushed in step 2, and control the kernel-shell ratio in the mixed mixture to be 8.5:1.5;

[0011] Step 4: Load the mixed materials in step 3 into a screw oil press for pressing, control the pressing temperature to be lower than 30° C., filter and separate to obtain Xanthoceras sorbifolia oil.

[0012] Preferably, before mixing the raw Xanthoceras sorbifolia and the fried cooked Xanthoceras sorbifolia, the raw materials need to be pretreated, and the pretreatment conditions are: removing the Xanthoceras sorbifolia with insect pests and shriveled, rinsing them with tap water, removing various impurities adhering to the surface, and drying them naturally.

[0013] Preferably, the irradiation treatment condition in step 1 is: 250 Gy dose.

[0014] Preferably, the frying temperature in step 1 is 140-150° C., and the frying time is 10-20 min.

[0015] Preferably, the pulverizing in step 2 pulverizes the seed kernels to a particle size of 24-40 mesh.

[0016] Preferably, the pulverizing in step 2 pulverizes the seed kernels to a particle size of 40 mesh.

[0017] Preferably, in the mixture, the mass ratio of the irradiated raw Xanthoceras sorbifolia to the fried cooked Xanthoceras sorbifolia is 1:1.

[0018] Beneficial effects of the present invention

[0019] The present invention provides a method for extracting Xanthoceras sorbifolia oil, which has the following advantages:

[0020] 1. Raw and cooked blending: increase frying oil rate

[0021] Processing of raw Xanthoceras sorbifolia: 60 When Co-γ rays are irradiated, it can change the physical properties of the material, making oil pressing smoother, improving efficiency and oil yield. In addition, irradiation pretreatment has strong bactericidal power, can inactivate microorganisms, ensure the hygiene and safety of oil pressing products, and avoid the use of chemical preservatives. And operating at room temperature can lock in the nutrition and flavor of the oil, increase efficiency for the enterprise, and meet the consumer's demand for healthy food.

[0022] Processing of ripe Xanthoceras sorbifolia: After drying, stir-fry at 140-150°C for 10-20 minutes to ensure that the seeds are heated evenly. High temperature destroys the cell structure of the seed kernel, enhances the fluidity of the oil, and increases the oil yield; antioxidants are activated and the shelf life is extended. After stir-frying, the seed kernel becomes loose, easy to squeeze and crush, the seed coat is easy to peel, and impurities are easy to sieve, which helps with subsequent processing.

[0023] 2. Whole fruit crushing: crush the kernel to 40 mesh size, which helps to increase the oil yield during pressing, while maintaining a certain particle size for subsequent oil residue separation.

[0024] 3. Accurate mixing ratio and pressing process

[0025] Mixing ratio: kernel-shell ratio is 8.5:1.5, that is, the kernel of Xanthoceras sorbifolia is the main raw material, and a small amount of shell is added. This ratio can fully utilize the oil in the kernel, and can also improve the stability and oil yield of the pressing process by taking advantage of the structural characteristics of the shell.

[0026] 4. Pressing process

[0027] Use a static pressure oil press for pressing, with about 500g of material loaded each time. The pressing temperature is controlled within 30℃. This low-temperature pressing method is conducive to fully extracting the oil from the Xanthoceras sorbifolia seeds, ensuring that the nervonic acid in the Xanthoceras sorbifolia kernel is not lost, reducing the oxidation of linoleic acid, preventing oxidative rancidity, and ensuring the quality and nutritional value of the oil.

[0028] 5. The method of the present invention can effectively retain the effective ingredients in Xanthoceras sorbifolia oil and the extraction rate is calculated as follows:

[0029] In summary, in the entire Xanthoceras sorbifolia oil extraction process of the present invention, the method can effectively retain various key active ingredients in Xanthoceras sorbifolia oil, which play an important role in the protection and repair process of vascular damage. In the zebrafish model experiment, the effect is quite significant. When Xanthoceras sorbifolia oil acts on zebrafish, the damaged blood vessels in its body can quickly recover to a near-normal state under the nourishment and repair of these active ingredients, showing a strong vascular repair potential, which provides a strong preliminary basis for further exploring its application in maintaining human vascular health.

[0030] This method also performs well in terms of economic benefits and safety. Compared with traditional extraction processes, its input costs are greatly reduced. Whether it is equipment purchase, raw material pretreatment, or subsequent processing links, costs are controllable through optimized processes and streamlined operations, making large-scale industrial production of Xanthoceras sorbifolia oil more feasible. On the other hand, from the extraction rate point of view, after precise calculation, an excellent value of up to 57.7% was obtained, which means that with the same raw material input, more high-quality Xanthoceras sorbifolia oil can be obtained, greatly improving resource utilization.

[0031] In addition, the use of harmful substances such as organic solvents is eliminated throughout the entire extraction process, completely avoiding the problem of organic solvent residues common in traditional extraction processes. The produced Xanthoceras sorbifolia oil is highly pure and safe for consumption. It can not only be used as a nutritious cooking oil for daily diet, but also provide high-quality raw material basis for health food, pharmaceutical research and development and other fields, expanding the application prospects of Xanthoceras sorbifolia oil in all directions, and is expected to inject new vitality into human health and industrial development in the future. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The present invention is a schematic flow chart of a method for extracting Xanthoceras sorbifolia oil in Example 1 of the present invention.

[0033] Figure 2 This is a comparison of the blood vessels in the lower intestine of zebrafish;

[0034] Figure 3 This is a comparison of the lengths of the inferior intestinal veins in zebrafish;

[0035] Figure 4 This is a bar graph showing the effect of Xanthoceras sorbifolia oil administration on the SOD activity level in zebrafish;

[0036] Figure 5 This is a bar graph showing the effect of Xanthoceras sorbifolia oil administration on the concentration of Apela protein in zebrafish. DETAILED DESCRIPTION

[0037] The present invention provides a method for extracting Xanthoceras sorbifolia oil, comprising:

[0038] Step 1: Process the raw Xanthoceras sorbifolia 60 irradiating the fruit with Co-γ rays to obtain irradiated raw Xanthoceras sorbifolia, frying the cooked Xanthoceras sorbifolia to obtain fried cooked Xanthoceras sorbifolia, and mixing the irradiated raw Xanthoceras sorbifolia and the fried cooked Xanthoceras sorbifolia to obtain a mixed raw material;

[0039] Step 2: crushing the mixed raw materials in step 1;

[0040] Step 3: Mix the kernels and shells of the Xanthoceras sorbifolia crushed in step 2, and control the kernel-shell ratio in the mixed mixture to be 8.5:1.5;

[0041] Step 4: Load the mixed materials in step 3 into a screw oil press for pressing, control the pressing temperature to be lower than 30° C., filter and separate to obtain Xanthoceras sorbifolia oil.

[0042] According to the present invention, the raw materials need to be pretreated before mixing the raw and cooked fruits. The pretreatment conditions are preferably: removing the insect-infested and shriveled Xanthoceras sorbifolia, washing them with tap water, removing various impurities adhering to the surface, and drying them naturally.

[0043] (1) Accurately remove insect-infested and shriveled Xanthoceras sorbifolia fruits because such fruits are likely to carry pathogenic microbial communities, such as some fungi and bacterial spores, and shriveled fruits have serious nutrient loss and significantly reduced oil content, which will have a negative impact on the subsequent oil quality and yield. Through strict screening, the purity of the raw materials and the oil yield potential are guaranteed from the source.

[0044] (2) Use tap water to rinse the surface of Xanthoceras sorbifolia. The presence of impurities will not only contaminate the oil and introduce odor, but may also wear out the equipment during the processing process, reducing the service life of the equipment. After rinsing, dry it naturally to avoid residual water stains that breed microorganisms or cause the fruit to mold.

[0045] According to the present invention, raw and cooked Xanthoceras sorbifolia are mixed in proportion. Before mixing, the raw Xanthoceras sorbifolia needs to be processed first. The specific processing method is preferably: select high-quality seeds, free of pests and diseases, mildew and well-matured. Dry in a ventilated sunny place, turn frequently until uniform, and then 60 Co-γ ray irradiation, the irradiation condition is preferably: 250Gy dose.

[0046] The present invention is achieved by 60 Co-γ ray irradiation pretreatment of oil-pressing materials changes its chemical structure by causing chemical reactions such as breaking and cross-linking of material molecules, and at the same time produces free radicals to further degrade the materials, thereby changing the physical properties of the materials, such as texture and hardness, making them more conducive to oil pressing, and improving oil pressing efficiency and oil yield. In addition, its high-energy gamma rays can penetrate microbial cells to destroy their DNA or RNA structure, effectively inactivate pathogens and corrupt microorganisms, reduce pollution, extend the shelf life of materials, and ensure the hygiene and safety of oil pressing products. Irradiation may also change the activity of enzymes in oil pressing materials, promote the decomposition and transformation of materials, and have a positive impact on the oil pressing process and oil quality. And this pretreatment is carried out at room temperature, which can retain the nutritional components of the materials and the flavor of the oil products, and reduce the oxidation and rancidity of the oil products.

[0047] According to the present invention, the preferred treatment of ripe Xanthoceras sorbifolia is as follows: select ripe and moderately ripe fruits and air-dry them, and turn them evenly. Air-drying further removes excess moisture, reduces the toughness of the fruit, facilitates subsequent fine processing operations, and reduces oil temperature fluctuations and energy loss during oil extraction caused by moisture. After drying, the seeds are drum-fried at 140-150°C for 10-20 minutes, and stirred continuously during frying. Select ripe and moderately ripe fruits and air-dry them, and fry them in a specific high temperature range. The high temperature softens and partially degrades polysaccharides such as pectin and cellulose in the cell walls of the fruit, destroys the integrity of the cells, and promotes the dissolution of oil from the cells; the protein denatures and condenses, reducing the viscous resistance during pressing; continuous stirring ensures uniform heating, avoids local overheating and causes scorching, which will produce harmful substances such as polycyclic aromatic hydrocarbons to contaminate the oil products, and ensures that the frying process accurately controls the physical and chemical properties of the fruit.

[0048] According to the present invention, the whole fruit is crushed to a particle size of 24-40 meshes by means of a wall-breaking machine, the seed kernel structure is refined, and the contact interface between the seed kernel and the oil pressing machine parts is greatly expanded. The oil storage unit inside the crushed small-particle seed kernel is more easily penetrated and squeezed by mechanical pressure, breaking the adsorption bond between the oil molecules and the surrounding tissues, creating favorable conditions for efficient oil pressing and significantly improving the oil extraction efficiency.

[0049] According to the present invention, the kernel-shell ratio is precisely set to 8.5:1.5, and the fruit shell is not a simple "filler" in the oil extraction process. First, the fruit shell has a certain mechanical strength, which can assist in supporting the pressure distribution in the initial stage of pressing, guide the oil flow, and optimize the oil extraction dynamics process; second, the fruit shell is rich in secondary metabolites such as flavonoids and saponins. The appropriate proportion of mixing can synergize with the kernel oil to enhance the antioxidant, anti-inflammatory and other health effects of the oil, enrich the nutritional content, and improve the overall quality of the product.

[0050] According to the present invention, a screw oil press is used for pressing operation, and its core principle is to gradually apply progressive pressure to the loaded raw materials based on the uniform rotation of the screw shaft. This pressure mode simulates the progressive extrusion process of manual oil pressing, which can gently and continuously promote the oil to seep out from the gaps between the seed kernel tissues, avoid instantaneous high pressure from damaging the oil molecular structure, and ensure the natural properties of the oil; the precise control of each loading of about 500g ensures the stability of the pressure field and temperature field inside the oil press, adapts the equipment to the optimal working parameters, and achieves efficient and stable oil output.

[0051] The pressing temperature is strictly controlled below 30°C. The low temperature environment effectively inhibits the biological activities of oil oxidase, fat hydrolase, etc., prevents the oil from oxidation, hydrolysis and deterioration at the moment of pressing, and retains the unsaturated fatty acids, vitamin E and other heat-sensitive nutrients in the oil to the greatest extent, ensuring the nutrition and flavor of the oil.

[0052] According to the present invention, a silver screen is used to perform the initial filtration process. Silver ions have certain antibacterial properties and can inhibit the growth of microorganisms during filtration, thereby reducing the risk of oil contamination. The screen is designed based on precise apertures to accurately intercept larger oil residue particles and preliminarily purify the oil.

[0053] The crude oil after initial filtration flows into the oil storage tank through the oil scraper. The unique flow-guiding structure of the oil scraper and the synergistic effect of gravity further enhance the oil-residue separation efficiency. Based on the difference in density and fluidity between oil and residue, the two can be efficiently separated and the residual micro-residue of oil can be reduced.

[0054] According to the present invention, the Xanthoceras sorbifolia seed kernel oil obtained by cold pressing needs to be stored in a relatively low temperature and light-proof environment. Temperature is a key factor affecting the oxidation stability of oils and fats. Under low temperature conditions, the thermal motion of oil molecules slows down, and the contact reaction rate with oxygen is greatly reduced, which effectively delays the oxidation process of oils and fats, and avoids the generation of odors and harmful substances caused by oil rancidity. Light, especially ultraviolet rays, has the energy to stimulate photochemical reactions of oil molecules. Light-proof storage can prevent the initiation of photooxidation chain reactions, ensure that the oil maintains good color, flavor and nutritional quality for a long time, effectively extend the shelf life, and ensure the quality stability of the product during the shelf life.

[0055] The present invention is further described in detail below in conjunction with specific embodiments. The present invention relates to a raw material of Xanthoceras sorbifolia seeds produced in Changchun, Jilin.

[0056] Comparative Example 1

[0057] Weigh 20g of crushed Xanthoceras sorbifolia seed kernels, wrap them with filter paper and place them in a Soxhlet extractor, take 150ml of anhydrous ethanol and place it in a round-bottom flask, pass condensed water into it, turn on the electric heating jacket and control its voltage at 100V, heat for 1h30min, and then rotary evaporate the liquid in the round-bottom flask in batches until no anhydrous ethanol remains, use a dropper to suck out the internal oil, place it in the bottle, weigh and record it.

[0058] The extraction rate of the product in Comparative Example 1:

[0059] Comparative Example 2

[0060] Weigh 20g of crushed Xanthoceras sorbifolia seed kernels, wrap them with filter paper and place them in a Soxhlet extractor, take 150ml of cyclohexane and place it in a round-bottom flask, pass condensed water, turn on the electric heating jacket and control its voltage at 150V, heat for 1h30min, then rotary evaporate the liquid in the round-bottom flask for 15min in batches, use a dropper to suck out the oil inside, place it in the bottle, weigh and record.

[0061] The extraction rate of the product in Comparative Example 2:

[0062] Comparative Example 3

[0063] Weigh 20g of crushed Xanthoceras sorbifolia seed kernels, wrap them with filter paper and place them in a Soxhlet extractor, take 150ml of petroleum ether and place it in a round-bottom flask, then pass condensed water into it, turn on the electric heating jacket and control its voltage at 150V, heat for 1h30min, centrifuge at 6000r for 20min, and then rotary evaporate for 30min. Use a dropper to suck out the internal oil, place it in a bottle, weigh and record it.

[0064] The extraction rate of the product in Comparative Example 3:

[0065] Example 1

[0066] Remove the insect-infested and shrunken Xanthoceras, rinse them with tap water, remove surface impurities and dry them naturally. Processing of raw and cooked Xanthoceras: Select high-quality seeds for raw Xanthoceras, place them in a ventilated sunny place to dry, turn them frequently until they are evenly distributed, and then irradiate them with 60Co-γ rays at a dose of 250Gy; for cooked Xanthoceras, select moderately mature fruits to dry, turn them evenly, and fry the seeds in a drum at 145°C for 15 minutes, stirring continuously during the period. Mix raw and cooked Xanthoceras in a mass ratio of 1:1, use a wall breaker to crush the whole fruit, crush the kernel to a particle size of 40 mesh, and ensure that the kernel-shell ratio is 8.5:1.5. Load the crushed Xanthoceras kernels into a screw oil press for pressing, with about 500 grams of material loaded each time, and control the pressing temperature below 30°C. The crude oil after pressing is first filtered with a silver screen, and then flows into the oil storage tank through an oil scraper for preliminary oil-residue separation. Finally, the cold-pressed Xanthoceras sorbifolia kernel oil is stored in a low temperature and light-proof environment. Figure 1 shown.

[0067] Extraction rate in Example 1:

[0068] Comparative Example 4

[0069] The experimental conditions and steps were the same as those in Example 1, except that the kernel-shell ratio was 8:2;

[0070] The extraction rate of comparative example 4:

[0071] Comparative Example 5

[0072] The experimental conditions and steps are the same as those in Example 1, except that the kernel-shell ratio is 9:1

[0073] The extraction rate of comparative example 5:

[0074] Comparative Example 6

[0075] The experimental conditions and steps are the same as those in Example 1, except that the raw and cooked Xanthoceras sorbifolia are mixed in a mass ratio of 1:2.

[0076] Extraction rate of comparative example 6:

[0077] Comparative Example 7

[0078] The experimental conditions and steps are the same as those in Example 1, except that the raw and cooked Xanthoceras sorbifolia are mixed in a mass ratio of 2:1.

[0079] The extraction rate of comparative example 7:

[0080] Efficacy verification of Xanthoceras sorbifolia oil:

[0081] Well-developed fluorescent zebrafish larvae were selected and cultured in 6-well plates, with 20 larvae in each well. The experiment set up a blank group, a PTK787 group (vatalani dihydrochloride, 0.2μg / mL PTK787 solution) and groups with different concentrations of Xanthoceras sorbifolia oil. After culturing for 24 hours with a cap and mark, the growth of the intestinal vein network of zebrafish in each group was observed under a fluorescence microscope and statistically analyzed. The zebrafish were anesthetized with MS-222 before taking pictures. The total length of the intestinal vein in each group of zebrafish after administration of Xanthoceras sorbifolia oil was measured and statistically analyzed. Figure 2 and 3 As shown, Figure 2 This is a comparison of the blood vessels in the intestine of zebrafish. Figure 3 The experimental results show that the Xanthoceras sorbifolia oil extracted by the present invention has a significant effect on angiogenesis at a concentration of 0 μg / ml-100 μg / ml. Therefore, Xanthoceras sorbifolia oil can restore the vascular damage caused by PTK787.

[0082] The zebrafish that had been photographed were ground into different groups and then tested with a BCA kit to obtain the protein concentration of each group. The activity of superoxide dismutase (SOD) was tested according to the instructions in the total superoxide dismutase kit. Figure 4 As shown, Figure 4 This is a bar graph showing the effect of Xanthoceras sorbifolia oil administration on SOD activity levels in zebrafish. The results show that after administration, the activity levels of superoxide dismutase in zebrafish increased to a certain extent, indicating that the ability of zebrafish to fight against secondary damage caused by free radicals increased, which can play an important role in the protection and repair of vascular damage in the organism.

[0083] Use the ground solutions of each group to make zebrafish Apela ELISA kit, such as Figure 5 As shown, Figure 5 This is a bar graph showing the effect of Xanthoceras sorbifolia oil administration on the concentration of Apela protein in zebrafish. Figure 5The data showed that the modeling group (PTK787) could reduce the Apela protein content, and Xanthoceras sorbifolia oil had a restorative effect on the PTK787-induced reduction in Apela protein content. Therefore, it can be concluded that Xanthoceras sorbifolia oil has a restorative effect.

Claims

1. A method for extracting Xanthoceras sorbifolia oil, characterized in that: include: Step 1: Process the raw Xanthoceras sorbifolia 60 irradiating the fruit with Co-γ rays to obtain irradiated raw Xanthoceras sorbifolia, frying the cooked Xanthoceras sorbifolia to obtain fried cooked Xanthoceras sorbifolia, and mixing the irradiated raw Xanthoceras sorbifolia and the fried cooked Xanthoceras sorbifolia to obtain a mixed raw material; Step 2: crushing the mixed raw materials in step 1; Step 3: Mix the kernels and shells of the Xanthoceras sorbifolia crushed in step 2, and control the kernel-shell ratio in the mixed mixture to be 8.5:1.5; Step 4: Load the mixed materials in step 3 into a screw oil press for pressing, control the pressing temperature to be lower than 30° C., filter and separate to obtain Xanthoceras sorbifolia oil.

2. The method for extracting Xanthoceras sorbifolia oil according to claim 1, wherein Before mixing the raw Xanthoceras sorbifolia and the fried cooked Xanthoceras sorbifolia, the raw materials need to be pretreated. The pretreatment conditions are: removing the Xanthoceras sorbifolia with insect pests and shriveled, washing them with tap water, removing various impurities adhering to the surface, and drying them naturally.

3. The method for extracting Xanthoceras sorbifolia oil according to claim 1, characterized in that: The irradiation treatment conditions described in step 1 are: 250Gy dose.

4. The method for extracting Xanthoceras sorbifolia oil according to claim 1, characterized in that: The frying temperature in step 1 is 140-150° C., and the frying time is 10-20 minutes.

5. The method for extracting Xanthoceras sorbifolia oil according to claim 1, characterized in that: In the mixture, the mass ratio of the irradiated raw Xanthoceras sorbifolia to the fried cooked Xanthoceras sorbifolia is 1:

1.

6. The method for extracting Xanthoceras sorbifolia oil according to claim 1, characterized in that: The crushing described in step 2 crushes the seed kernel to a particle size of 24-40 mesh.

7. The method for extracting Xanthoceras sorbifolia oil according to claim 1, characterized in that: The pulverizing described in step 2 pulverizes the seed kernel to a particle size of 40 mesh.

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