Interstitial fluid glucose colorimetric detection device and method

Abstract

The invention discloses a colorimetric detection device and method for glucose in tissue fluid. The colorimetric detection device comprises a tissue fluid extraction and detection module, a wireless receiving and control circuit module and a power supply module, the tissue fluid extraction and detection module comprises a shell, a tissue fluid extraction module, a detection chamber and an injection micropump; a glucose colorimetric detection sheet is arranged at the top in the detection chamber; the top of the detection chamber is connected to the injection micropump through a hose, positive pressure or negative pressure is provided for the detection chamber, and the tissue fluid extraction module is used for collecting the tissue fluid; the wireless receiving and control circuit module is used for receiving an instruction from a control end and controlling the tissue fluid extraction and detection module to automatically complete tissue fluid extraction and detection; the power module is used for providing power for the device. According to the technical scheme, safe, rapid and controlled extraction of the tissue fluid is achieved in a trace and local mode, the glucose concentration of the tissue fluid in a life body is rapidly detected, and the safety risk caused by blood drawing and the accuracy limitation of sweat detection are avoided.

Description

Technical Field

[0001] The present invention relates to the field of biomedical technology, and in particular to a device and method for colorimetric detection of glucose in tissue fluid. Background Art

[0002] Diabetes is a serious chronic disease, and its complications are the main cause of disability and death. Currently, the treatment of diabetes is to test glucose concentration through fingertip or venous blood sampling to guide patients' medication. Long-term invasive blood sampling brings great pain to patients. Sweat and saliva are very attractive for non-invasive monitoring of diabetes, but the glucose content in these body fluids is not highly correlated with blood sugar. The glucose level in tissue fluid is closely related to the blood sugar level. Glucose extracted from the tissue fluid around the skin may provide more information for disease diagnosis and monitoring the body state of diabetic patients.

[0003] Glucose in tissue fluid can be detected by colorimetric detection method, which has the advantages of visualization and high biosafety. However, in the prior art, when performing colorimetric detection, the tissue fluid needs to be extracted and transferred to a specific container for detection, which is easy to cause contamination during the transfer of the tissue fluid and takes a long time, and it is impossible to achieve in vivo and rapid colorimetric detection of glucose in tissue fluid. Summary of the invention

[0004] In order to solve the above-mentioned deficiencies and defects, a device and method are needed that can realize in vivo and rapid colorimetric detection of glucose in tissue fluid.

[0005] A first aspect of an embodiment of the present invention discloses an interstitial fluid glucose colorimetric detection device, comprising an interstitial fluid extraction detection module, and a wireless receiving and control circuit module and a power supply module fixed on the top of the interstitial fluid extraction detection module.

[0006] The tissue fluid extraction and detection module is used to extract tissue fluid from the body surface of the object being detected and perform detection; the wireless receiving and control circuit module is used to receive instructions from the control end, and control the tissue fluid extraction and detection module to automatically complete tissue fluid extraction and detection; the control end represents the control terminal used by the operator, which can be a smart phone or other smart terminal; the control end is configured with a camera and glucose colorimetric detection software to realize the recognition of glucose colorimetric detection results.

[0007] The tissue fluid extraction detection module includes a shell, and a tissue fluid extraction module is arranged on the bottom surface of the shell. The bottom surface represents the side in contact with the skin of the object to be detected. When working, the tissue fluid extraction module is attached to the body surface of the object to be detected and penetrates the body surface to extract tissue fluid.

[0008] A detection chamber is arranged inside the shell, and a glucose colorimetric detection sheet is arranged on the top of the detection chamber; an injection micropump is connected to the top of the detection chamber through a hose, and the injection micropump is used to control the air pressure in the detection chamber. When the detection chamber is under negative pressure, tissue fluid on the body surface is sucked into the detection chamber.

[0009] The wireless receiving and control circuit module includes a wireless receiving module, a microcontroller and a control circuit; the microcontroller is electrically connected to the wireless receiving module, the injection micropump and the control circuit, and is used to coordinate the various parts of the control device to complete the extraction and detection of tissue fluid; the wireless receiving module is used to receive wireless control commands from the control end to realize data interaction with the control end.

[0010] The power supply module is electrically connected to the wireless receiving and control circuit module and the injection micro pump to provide electrical energy.

[0011] As an optional embodiment, in the first aspect of the embodiment of the present invention, the tissue fluid extraction module is a hollow microneedle array, and the hollow microneedle array includes M×N hollow microneedles; the needle body of the hollow microneedle is hollow, and the base of the hollow microneedle is connected to the detection chamber; the hollow microneedle array is evenly distributed below the detection chamber; the value range of M is 10 to 12, and the value range of N is 10 to 12, which can be determined according to actual applications.

[0012] As an optional embodiment, in the first aspect of the embodiment of the present invention, the hollow microneedle is conical, with a bottom width of 450 to 550 microns, a height of 950 to 1050 microns, and a needle tip spacing of 1.9 to 2.1 mm; the hollow pore diameter of the hollow microneedle is 120 to 130 microns.

[0013] As an optional implementation, in the first aspect of the embodiment of the present invention, the detection chamber has a length of 24.5 to 25.5 mm, a width of 24.5 to 25.5 mm, and a height of 450 to 550 μm.

[0014] As an optional embodiment, in the first aspect of the embodiment of the present invention, the glucose colorimetric detection sheet is prepared by immersing a paper sheet in a solution containing GOx / HRP (glucose oxidase / horseradish peroxidase) and TMB (3,3',5,5'-tetramethylbenzidine).

[0015] As an optional implementation, in the first aspect of the embodiment of the present invention, the shell of the tissue fluid extraction detection module, the tissue fluid extraction module and the detection chamber are prepared as a whole by 3D printing with biocompatible resin.

[0016] As an optional implementation, in the first aspect of the embodiment of the present invention, the wireless receiving module adopts a 433 MHz frequency band and has a power consumption of no more than 12 mW; the microcontroller is a single chip microcomputer with a power consumption of no more than 10 mW.

[0017] As an optional implementation, in the first aspect of the embodiment of the present invention, the power module uses a 3.7V lithium battery as a power supply, the capacity of the lithium battery is not less than 300 mAh, and the number of charge and discharge cycles is not less than 100 times; the power module also includes a battery charge and discharge management circuit, which is used for charging management and over-discharge prevention management of the lithium battery.

[0018] The second aspect of the embodiment of the present invention discloses a method for colorimetric detection of glucose in tissue fluid, which is applied to the device for colorimetric detection of glucose in tissue fluid disclosed in the first aspect of the embodiment of the present invention. The method comprises: The tissue fluid glucose colorimetric detection device is attached to the smooth body surface of the subject to be detected, so that the tissue fluid extraction module is in contact with the body surface; Apply pressure to pierce the tissue fluid extraction module into the skin; The wireless receiving module receives the start command sent by the control end and transmits it to the microcontroller; the control end represents the control terminal used by the operator; the start command represents the start detection instruction; According to the start command, the microcontroller controls the injection micropump to start working, providing negative pressure for the detection chamber, and extracting the tissue fluid in the skin of the detected object into the detection chamber through the tissue fluid extraction module; After the tissue fluid enters the detection chamber, the glucose in the tissue fluid contacts the glucose colorimetric detection sheet and is oxidized by the GOx on the glucose colorimetric detection sheet to produce hydrogen peroxide. The hydrogen peroxide and the HRP on the glucose colorimetric detection sheet jointly catalyze the color development of TMB on the glucose colorimetric detection sheet. The control end is used to identify the TMB color development to obtain the glucose concentration of the tissue fluid.

[0019] Compared with the prior art, the embodiments of the present invention have the following beneficial effects: 1. The present invention adopts a hollow microneedle and negative pressure to extract tissue fluid, achieving safe, rapid and controlled extraction of tissue fluid in a trace and local manner, avoiding the safety risks brought by blood drawing and the accuracy limitations of sweat testing.

[0020] 2. The present invention adopts the colorimetric method to detect glucose, which has the advantages of being fast, convenient and visual, and avoids the biosafety risks and stability problems caused by the use of electrolytes in electrochemical detection.

[0021] 3. The present invention adopts a wireless receiving module to receive, process and output signals, thereby realizing a remote control function. It can perform detection in a situation where the collected object is not easily aware of it, thus avoiding causing discomfort to the collected object.

[0022] In summary, the present invention realizes rapid detection of glucose in living skin tissue fluid by introducing a glucose colorimetric detection device, automatically controls tissue fluid extraction, and processes and feeds back the detection signal to obtain glucose concentration. These functions greatly promote the research and application of in vivo detection technology in clinical diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 A schematic diagram of the structure of a tissue fluid glucose colorimetric detection device disclosed in an embodiment of the present invention; Figure 2 A schematic diagram of a hollow microneedle for extracting tissue fluid disclosed in an embodiment of the present invention; Figure 3 The present invention discloses a working flow chart of a transceiver and control circuit module.

[0024] Reference numerals and descriptions: 100-housing; 101-tissue fluid extraction module; 102-detection chamber; 103-injection micro pump; 104-glucose colorimetric detection sheet; 105-vent; 200-wireless receiving and control circuit module; 201-wireless receiving module; 202-microcontroller; 203-control circuit; 300-power module. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] Embodiment 1 See also Figure 1 . Figure 1 FIG. 1 is a schematic diagram of the structure of a tissue fluid glucose colorimetric detection device disclosed in an embodiment of the present invention. Figure 1As shown, an interstitial fluid glucose colorimetric detection device disclosed in an embodiment of the present invention includes: an extraction detection module, and a transceiver and control circuit module 200 and a power module 300 fixed on the top of the extraction detection module; the extraction detection module is used to extract skin interstitial fluid from the body surface of the detection object and perform detection. The wireless receiving and control circuit module 200 is used to receive instructions from the control end, and control the interstitial fluid extraction detection module to automatically complete the extraction and detection of interstitial fluid; the control end represents the control terminal used by the operator; the control end is configured with a camera and glucose colorimetric detection software to realize the recognition of the glucose colorimetric detection result.

[0027] The extraction and detection module includes a shell 100; a tissue fluid extraction module 101 is arranged on the bottom surface of the shell 100; the bottom surface represents the side in contact with the skin of the object to be detected; when working, the tissue fluid extraction module 101 is attached to the body surface of the object to be detected, and when tissue fluid needs to be extracted, the tissue fluid extraction module 101 is inserted into the body surface; a detection chamber 102 is arranged inside the shell; the detection chamber 102 is connected to the tissue fluid extraction module 101; a vent 105 is arranged on the top of the detection chamber, and the vent 105 is connected to an injection micropump 103 through a hose (not shown in the figure); the injection micropump 103 is fixed on the top outside the shell; the injection micropump 103 is used to provide negative pressure for the detection chamber 102, and after the detection chamber 102 is under negative pressure, tissue fluid is extracted into the detection chamber 102 through the tissue fluid extraction module 101.

[0028] A glucose colorimetric detection piece 104 is disposed at the top of the detection chamber 102; a detection piece replacement port is disposed at the side of the detection chamber 102, so that an old detection piece can be pulled out and a new detection piece can be inserted.

[0029] The wireless receiving and control circuit module 200 and the power module 300 are fixed on the top of the housing 100; the wireless receiving and control circuit module 200 is electrically connected to the injection micro pump 103 and the power module 300 through a flexible flat cable. The wireless receiving and control circuit module 200 is used to receive instructions from the user, and the control device performs tissue fluid extraction and detection according to a preset program.

[0030] The transceiver and control circuit module 200 includes a wireless receiving module 201, a microcontroller 202 and a control circuit 203; the microcontroller 202 is electrically connected to the wireless receiving module 201, the control circuit 203 and the injection micropump 103; the wireless receiving module 201 is used to receive wireless control commands from the control end; the control end represents the control terminal used by the operator.

[0031] The power module 300 is electrically connected to the wireless receiving and control circuit module 200 and the injection micro pump 103 to provide power to the device.

[0032] It can be seen that the tissue fluid glucose colorimetric detection device described in this example adopts a negative pressure method to extract tissue fluid, and realizes safe, rapid and controlled extraction of tissue fluid in a trace and local manner, which solves the safety risks caused by blood drawing and the accuracy limitations of sweat detection. It realizes integrated tissue fluid extraction and glucose detection, which greatly increases the efficiency of detection and the portability of the equipment. It can quickly detect glucose in the body's tissue fluid. The operation process is simple and convenient, short in time, and reduces the discomfort of the subject being collected.

[0033] In another optional embodiment, Figure 2 As shown, the tissue fluid extraction module 101 is a hollow microneedle array; the hollow microneedle array includes M×N hollow microneedles; the needle body of the hollow microneedle is hollow, and the base of the hollow microneedle is connected to the detection chamber; the hollow microneedle array is evenly distributed below the detection chamber; the value range of M is 10-12, and the value range of N is 10-12.

[0034] like Figure 2 As shown, the hollow microneedle array in this embodiment adopts an 11×11 hollow microneedle sequence, which can meet the amount of tissue fluid required for detection and is not likely to cause discomfort to the subject being collected.

[0035] It should be noted that the tissue fluid glucose colorimetric detection device described in this example collects tissue fluid in a minimally invasive manner, which reduces discomfort to the subject being detected and has the advantages of simplicity and hygiene.

[0036] In another optional embodiment, the hollow microneedle is conical, with a bottom width of 450 to 550 microns, a height of 950 to 1050 microns, and a needle tip spacing of 1.9 to 2.1 mm; the hollow hole diameter of the hollow microneedle is 120 to 130 microns, and the distance between the hole center and the hollow microneedle center is 120 to 130 microns.

[0037] Preferably, the bottom width of the hollow microneedle is 500 microns and the height is 1000 microns; the center spacing of the microneedles of the hollow microneedle array is 2 mm; the hollow hole diameter of the hollow microneedle is 125 microns, and the distance between the hole center and the hollow microneedle center is 125 microns.

[0038] In yet another optional embodiment, the detection chamber 102 has a length of 24.5 to 25.5 mm, a width of 24.5 to 25.5 mm, and a height of 450 to 550 μm.

[0039] Preferably, the length and width of the detection chamber 102 are both 25 mm, and the height is 500 μm.

[0040] In another optional embodiment, the glucose colorimetric detection sheet 104 is prepared by immersing a paper sheet in 5 mL of an aqueous solution containing 625 U / LGOx / HRP (glucose oxidase / horseradish peroxidase) and 3.33 mM TMB (3,3',5,5'-tetramethylbenzidine) for 5 minutes and then drying at room temperature for 2 hours.

[0041] In yet another optional embodiment, the glucose colorimetric detection strip 104 has a length of 19.5 to 20.5 mm and a width of 14.5 to 15.5 mm.

[0042] Preferably, the glucose colorimetric detection strip 104 has a length of 20 mm and a width of 15 mm.

[0043] It should be noted that the interstitial fluid glucose colorimetric detection device described in this example can be conveniently used by using the glucose colorimetric detection sheet provided in this embodiment, and can quickly and accurately complete the glucose detection.

[0044] In another optional embodiment, the extraction detection module 100 is made of biocompatible resin (BIO) through 3D printing as a whole. The various parts of the extraction detection module 100 are integrated, without gaps, and no air leakage occurs, making the operation of the device more accurate and the monitored object feel more comfortable. The tissue fluid extraction detection module prepared with biocompatible resin has good biocompatibility and corrosion resistance, making the detection results more accurate; it has good compression and bending resistance, and is more suitable for the body of the collected object.

[0045] In another optional embodiment, the wireless receiving module 201 uses a 433MHz frequency band and has a power consumption of no more than 12mW; the microcontroller 202 is a single-chip microcomputer with a power consumption of no more than 10mW. The use of a low-power microcontroller is conducive to miniaturization of the device and prolonging the working time.

[0046] The wireless receiving module 201 is designed and implemented based on a dedicated receiving chip in the 433MHz frequency band, and cooperates with EMC / EMI design to ensure the reliability and accuracy of wireless signal reception; the demodulated signal of the wireless receiving module 201 is transmitted to the I / O port of the microcontroller, and the software signal is reconstructed by the microcontroller 202, and the signal is decoded according to the protocol layer of the wireless communication to obtain relevant control instructions. According to the relevant operation instructions, the microcontroller 202 can realize the rapid electric control tissue fluid extraction operation of the tissue fluid extraction module 101 by controlling the current size.

[0047] It should be noted that the tissue fluid cortisol detection device provided in this application can be used for humans and animals. This application uses a wireless transceiver module to receive, process and output signals, realizes the wireless transceiver function under the 433MHz frequency band, can realize long-distance control, and can collect tissue fluid even when not close to the object being detected. The 433MHz frequency band is selected for communication, which has the characteristics of high reliability, high stability, strong anti-interference ability, strong penetration ability and strong environmental adaptability.

[0048] In another optional embodiment, the power module 300 includes a lithium battery and a charge and discharge management circuit; the lithium battery is a power supply, the capacity of the lithium battery is not less than 300 mAh, and the number of charge and discharge cycles at 23±2°C 0.5C is not less than 100 times, which increases the service life and life of the device; the use of lithium batteries can effectively reduce the weight of the device and has good working stability; The charge and discharge management circuit is used for charging management and over-discharge prevention management of lithium batteries; the charge and discharge management circuit boosts the original 3.7V lithium battery voltage, and adjusts the effective range of the boost amplitude through the output of the D / A converter of the microcontroller, thereby achieving the purpose of adjusting the current intensity. The voltage is applied to the injection micropump after passing through the MOS tube adjustment circuit. At this time, the MOS tube is controlled to be on and off through the PWM signal of the microcontroller, thereby achieving effective regulation of the working parameters of the injection micropump, such as pressure, flow rate, and duration.

[0049] Embodiment 2 This embodiment provides a method for colorimetric detection of glucose in tissue fluid, which is applied to the device for colorimetric detection of glucose in tissue fluid disclosed in the first embodiment of the present invention. The method includes: The tissue fluid glucose colorimetric detection device is attached to the smooth body surface of the subject to be detected, so that the tissue fluid extraction module 101 is in contact with the body surface; Apply pressure to pierce the microneedle array of the tissue fluid extraction detection module 101 into the skin; like Figure 3 As shown, the wireless receiving module 201 receives the start command sent by the control end and transmits it to the microcontroller 202; the control end represents the control terminal used by the operator; the start command represents the start detection instruction; According to the start command, the microcontroller 202 controls the injection micropump 103 to start working, providing negative pressure for the detection chamber 102, and extracting the tissue fluid in the skin of the detected object from the tissue fluid extraction module 101 to the detection chamber 102; The glucose colorimetric detection sheet 104 in the detection chamber 102 reacts with the tissue fluid, and the glucose in the tissue fluid is oxidized by GOx (glucose oxidase) on the glucose colorimetric detection sheet 104 to produce hydrogen peroxide, and the hydrogen peroxide and the HRP (horseradish peroxidase) on the glucose detection sheet 104 jointly catalyze the color development of TMB (3,3',5,5'-tetramethylbenzidine) on the glucose detection sheet; The camera at the control end receives the color signal, processes and converts the signal through software, and outputs the measured glucose concentration.

[0050] Embodiment 3 This embodiment provides a hollow microneedle detection method.

[0051] 1. Detection method of hollow microneedle array.

[0052] The morphology of the prepared glucose colorimetric detection microneedle array was characterized by scanning electron microscopy, stereo microscope and optical microscope. The microneedles were pasted on the sample stage, sprayed with gold and purged with nitrogen (this is the detection method), and the microneedle morphology was observed and the size was measured using a scanning electron microscope, and the microneedle morphology was further observed under a stereo microscope and an optical microscope.

[0053] 2. Microneedle tissue fluid extraction behavior test.

[0054] The frozen ex vivo SD rat skin was removed and immersed in physiological saline to return to room temperature. The surface moisture of the skin was gently wiped with gauze. The microneedle was vertically inserted into the rat skin using a universal tensile testing machine, and negative pressure was applied to the detection chamber to extract tissue fluid. The microneedle was removed after 1 minute, and the weight of the microneedle before and after tissue fluid extraction was weighed using a balance to calculate the amount of tissue fluid extracted. The intensity and time of negative pressure application during microneedle application were evaluated by measuring the tissue fluid extraction behavior of microneedles inserted into the ex vivo SD rat skin. The microneedle was vertically inserted into the rat skin, and the microneedle was removed after applying negative pressure for 0 to 10 minutes. The weight of the microneedle before and after tissue fluid extraction was weighed using a balance, and the microneedle tissue fluid extraction behavior was evaluated by calculating the weight difference.

[0055] 3. Evaluation of detection effect The detection accuracy and sensitivity of the glucose colorimetric detection microneedle array were evaluated by using biological detection techniques such as enzyme-linked immunosorbent assay (ELISA) and a commercially available blood glucose meter to detect the glucose content in mouse skin tissue fluid and blood, thereby demonstrating the biological effectiveness of the device.

[0056] It should be noted that when an element is referred to as being "fixed on", "provided with" or "connected to" another element, it can be directly on the other element or there can be a central element. "Fixed on", "provided on" or "connected to" can be a fixed connection, a threaded connection, an adhesive connection, a snap connection or one or more connection methods selected according to actual conditions. An element can be understood as a component or a part. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and do not represent the only implementation method.

[0057] Finally, it should be noted that the tissue fluid glucose colorimetric detection device and method disclosed in the embodiments of the present invention only disclose the preferred embodiments of the present invention, which are only used to illustrate the technical scheme of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, it should be understood by those skilled in the art that the technical schemes described in the aforementioned embodiments can still be modified, or some of the technical features therein can be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical schemes from the spirit and scope of the technical schemes of the embodiments of the present invention.

Claims

1. A tissue fluid glucose colorimetric detection device, characterized in that: It includes a tissue fluid extraction and detection module, and a wireless receiving and control circuit module and a power supply module fixed on the top of the tissue fluid extraction and detection module; The tissue fluid extraction detection module comprises a shell, the bottom surface of the shell is provided with a tissue fluid extraction module, the bottom surface represents the side in contact with the skin of the detected object, and the tissue fluid extraction module is attached to the body surface of the detected object during operation; a detection chamber is provided inside the shell; a glucose colorimetric detection sheet is provided on the top of the detection chamber; an injection micro pump is connected to the top of the detection chamber through a hose, and the injection micro pump is fixed to the top of the tissue fluid extraction detection module; The wireless receiving and control circuit module includes a wireless receiving module, a microcontroller and a control circuit; the microcontroller is electrically connected to the wireless receiving module, the injection micropump and the control circuit; The power supply module is electrically connected to the wireless receiving and control circuit module and the injection micro pump to provide electrical energy.

2. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The tissue fluid extraction module is a hollow microneedle array; the needle body of the hollow microneedle is hollow, and the base of the hollow microneedle is connected to the detection chamber; The hollow microneedle array is evenly distributed below the detection chamber.

3. The tissue fluid glucose colorimetric detection device according to claim 2, characterized in that: The hollow microneedle is conical, with a bottom width of 450 to 550 microns, a height of 950 to 1050 microns, and a needle tip spacing of 1.9 to 2.1 millimeters; the hollow pore diameter of the hollow microneedle is 120 to 130 microns.

4. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The detection chamber has a length of 24.5 to 25.5 mm, a width of 24.5 to 25.5 mm, and a height of 450 to 550 μm.

5. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The glucose colorimetric detection sheet is prepared by immersing a paper sheet in a solution containing GOx / HRP and TMB.

6. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The shell, tissue fluid extraction module and detection chamber of the tissue fluid extraction detection module are prepared as a whole by 3D printing with biocompatible resin.

7. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The wireless receiving module adopts the 433MHz frequency band and has a power consumption of no more than 12mW; the microcontroller is a single chip microcomputer with a power consumption of no more than 10mW.

8. The tissue fluid glucose colorimetric detection device according to claim 1, characterized in that: The power module uses a 3.7V lithium battery as a power supply. The capacity of the lithium battery is not less than 300 mAh, and the number of charge and discharge cycles is not less than 100 times. The power module also includes a battery charge and discharge management circuit.

9. A method for colorimetric detection of glucose in tissue fluid, characterized in that: Applied to the tissue fluid glucose colorimetric detection device according to any one of claims 1 to 8, the method comprising: The tissue fluid glucose colorimetric detection device is attached to the smooth body surface of the subject to be detected, so that the tissue fluid extraction module is in contact with the body surface; Apply pressure to pierce the tissue fluid extraction module into the skin; The wireless receiving module receives the start command sent by the control end and transmits it to the microcontroller; the control end represents the control terminal used by the operator; the start command represents the start detection instruction; According to the start command, the microcontroller controls the injection micropump to start working, providing negative pressure for the detection chamber, and extracting the tissue fluid in the skin of the detected object into the detection chamber through the tissue fluid extraction module; After the tissue fluid enters the detection chamber, the glucose in the tissue fluid contacts the glucose colorimetric detection sheet and is oxidized by the GOx on the glucose colorimetric detection sheet to produce hydrogen peroxide. The hydrogen peroxide and the HRP on the glucose colorimetric detection sheet jointly catalyze the color development of TMB on the glucose colorimetric detection sheet. The control end is used to identify the TMB color development to obtain the glucose concentration of the tissue fluid.

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