Thin-layer identification method for peach kernels and bitter apricot kernels

Abstract

According to the thin-layer identification method for the peach kernels and the bitter apricot kernels, the unique combination of an extraction method, a developing solvent, a color developing agent and the like is adopted, the peach kernels and the bitter apricot kernels can be rapidly and effectively identified, and the method can save cost, is high in precision and sensitivity, good in stability, convenient and rapid to use and capable of accurately identifying the peach kernels and the bitter apricot kernels; good application prospects are realized.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine identification, in particular to a thin layer identification method for peach kernel and bitter almond. Background Art

[0002] Peach kernel and bitter almond belong to the same plant family. Their properties and ingredients are very similar, but their functions and indications are different. Peach kernel has the effects of promoting blood circulation and removing blood stasis, moistening the intestines and relieving constipation, and relieving cough and asthma. It is used for amenorrhea, dysmenorrhea, lumps and masses, lung abscesses and intestinal abscesses, injuries from falls, constipation due to dryness of the intestines, and cough and asthma. Bitter almond has the effects of lowering qi, relieving cough and relieving asthma, and moistening the intestines and relieving constipation. It is used for cough and asthma, chest fullness and phlegm, and constipation due to dryness of the intestines. The two cannot be used together, but because the price of bitter almonds is lower than that of peach kernels, there is a phenomenon of bitter almonds being mixed with peach kernels for sale.

[0003] At present, the TLC identification methods for peach kernels and bitter almonds in the Chinese Pharmacopoeia and local standards all use amygdalin as a reference. Amygdalin is a common component of peach kernels and bitter almonds, and the two cannot be identified. No TLC identification method for peach kernels and bitter almonds has been published yet. Summary of the invention

[0004] The invention provides a thin layer identification method for peach kernels and bitter almonds. The method can quickly and effectively identify peach kernels and bitter almonds, save costs, and has high precision and sensitivity, good stability, and can accurately identify peach kernels and bitter almonds.

[0005] The present invention provides a thin layer identification method for peach kernels and bitter almonds, comprising the following steps:

[0006] Take peach kernel and bitter almond to prepare peach kernel and bitter almond solution;

[0007] Peach kernel and bitter almond solution were aspirated and tested by TLC method;

[0008] The developing solvents used in the thin layer identification method are toluene, ethyl acetate and methanol.

[0009] The volume ratio of toluene, ethyl acetate and methanol in the developing solvent is (13-17):(1-3):(1-3).

[0010] Preferably, the volume ratio of toluene, ethyl acetate and methanol in the developing solvent is 15:2:2.

[0011] Preferably, the preparation method of the peach kernel and bitter almond solution is: respectively taking peach kernel and bitter almond medicinal material powders, respectively adding ethanol, ultrasonicating, filtering, evaporating to dryness, adding ethanol to dissolve, and obtaining the peach kernel and bitter almond solution.

[0012] Preferably, the volume fraction of the ethanol added for the first time is 65-75%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material powder to the ethanol added for the first time is 1 / 45-55, in g / ml.

[0013] Preferably, the volume fraction of the ethanol added for the first time is 70%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material to the ethanol added for the first time is 1 / 50, in units of g / ml.

[0014] Preferably, the volume fraction of the ethanol added for the second time is 65-75%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material powder to the ethanol added for the second time is 1 / 1-3, in g / ml;

[0015] Preferably, the volume fraction of the ethanol added for the second time is 70%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material to the ethanol added for the second time is 1 / 2, in units of g / ml.

[0016] Preferably, the power of the ultrasound is 250W, the frequency is 40kHz, the ultrasound time is 30 minutes, and the filtration is performed using medium-speed double-circle qualitative filter paper, that is, the pore size is 30 to 50 microns.

[0017] Preferably, the thin layer identification method is: spotting peach kernel and bitter almond solution on a thin layer plate, developing with a developing agent, taking out, drying, and inspecting under ultraviolet light;

[0018] Further preferably, the thin layer plate is an ordinary thin layer plate made of silica gel.

[0019] Preferably, the weight-to-volume ratio of the peach kernel or bitter almond medicinal powder to the spotting amount is 1 / 4-6, expressed in g / μL, and the sample is spotted on the thin layer plate in a strip shape with a strip width of 9-11 mm. The distance between the spotting origin and the lower edge of the thin layer plate is 9-11 mm, and after spotting, the sample is heated on a thin layer heating plate at 40-50°C for 10-20 min.

[0020] Further preferably, the weight-to-volume ratio of the peach kernel or bitter almond medicinal powder and the spotting amount is 1 / 5, expressed in g / μL, and the sample is spotted on the thin layer plate in a strip shape with a strip width of 10 mm. The distance between the spotting origin and the lower edge of the thin layer plate is 10 mm, and after spotting, the sample is heated on a thin layer heating plate at 45°C for 15 min.

[0021] Preferably, the developer is a 10% sulfuric acid ethanol solution, the heating is performed at 105° C. for 3 minutes, and the wavelength of the inspection ultraviolet lamp is 366 nm.

[0022] The present invention has the following beneficial effects:

[0023] The peach kernel and bitter almond thin layer identification method adopted by the present invention can save costs and be faster and simpler compared with other chromatographic identifications such as liquid chromatography, gas chromatography, etc. by adopting a silica gel G thin layer plate; in addition, the solvent used by the developing agent of the present invention has good separation degree, and the spots of bitter almonds can be separated from the spots of other components, so that the peach kernel and bitter almonds can be quickly identified; the color developer can clearly display the characteristic spots of the peach kernel and bitter almonds; a unique extraction method+developing agent+developing agent is used to form a complete peach kernel and bitter almond thin layer identification method; the method has high overall precision and sensitivity, good stability, strong specificity, is convenient and fast, and has good application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The inspection results are obtained by using the thin layer identification method of peach kernel and bitter almond in Example 1 of the present invention under ultraviolet light with a wavelength of 366 nm. 1-7: peach kernel medicinal material; 8-11: bitter almond medicinal material.

[0025] Figure 2 The results of the inspection under a UV light with a wavelength of 336 nm using the thin layer identification method of Example 2 of the present invention in which different proportions of bitter almonds are added to peach kernels. 1: peach kernel medicinal material; 2: peach kernel medicinal material (90% by weight) bitter almond medicinal material (10% by weight); 3: peach kernel medicinal material (80% by weight) bitter almond medicinal material (20% by weight); 4: peach kernel medicinal material (70% by weight) bitter almond medicinal material (30% by weight); 5: peach kernel medicinal material (60% by weight) bitter almond medicinal material (40% by weight); 6: peach kernel medicinal material (50% by weight) bitter almond medicinal material (50% by weight); 7: peach kernel medicinal material (40% by weight) bitter almond medicinal material (60% by weight); 8: bitter almond medicinal material.

[0026] Figure 3 The results of comparative example 1 using the thin layer identification method of peach kernel and bitter almond in Example 1 of the present invention but using petroleum ether II-chloroform-acetone as the developing agent under ultraviolet light with a wavelength of 366 nm are shown. 1: bitter almond control extract; 2-3: bitter almond medicinal material; 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0027] Figure 4 This is the inspection result of comparative example 2 using the thin layer identification method of peach kernel and bitter almond in example 1 of the present invention, but using toluene-ethyl acetate-anhydrous ethanol (volume ratio of 15:1:4) as the developing agent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0028] Figure 5This is the inspection result of comparative example 3 using the thin layer identification method of peach kernel and bitter almond in Example 1 of the present invention, but using toluene-butyl acetate-methanol (volume ratio of 15:1:4) as the developing agent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0029] Figure 6 This is the inspection result of comparative example 4 using the thin layer identification method of peach kernel and bitter almond in example 1 of the present invention, but using toluene-isopropanol-methanol (volume ratio of 15:1:4) as the developing agent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0030] Figure 7 This is the inspection result of comparative example 5 using the thin layer identification method of peach kernel and bitter almond in Example 1 of the present invention, but using toluene-butyl acetate-anhydrous ethanol (volume ratio of 15:1:4) as the developing agent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0031] Figure 8 This is the inspection result of comparative example 6 using the thin layer identification method of peach kernel and bitter almond in example 1 of the present invention, but using toluene-ethyl acetate-methanol (volume ratio of 15:3:3) as the developing solvent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material.

[0032] Fig. 9 This is the inspection result of comparative example 7 using the thin layer identification method of peach kernel and bitter almond in Example 1 of the present invention, but using toluene-ethyl acetate-methanol (volume ratio of 15:3:2) as the developing solvent under ultraviolet light with a wavelength of 366nm. Among them, 1: bitter almond control extract; 2-3: bitter almond medicinal material, 4: peach kernel control extract; 5-6: peach kernel medicinal material. DETAILED DESCRIPTION

[0033] The following are detailed descriptions of embodiments of the present invention and comparative examples. The embodiments and comparative examples described below are exemplary and are only used to explain the present invention, and should not be construed as limiting the present invention. Where specific techniques or conditions are not specified in the embodiments and comparative examples, the techniques or conditions described in the literature in this area or the product specifications are used. Where the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be obtained commercially. Where percentages or ratios are not specified, they are all volume percentages. The term "and / or" as used herein includes any and all combinations of one or more related listed items.

[0034] Apparatus and Materials:

[0035] ATS 4 TLC automatic sample spotter (CAMAG, Switzerland), TLC double-slot developing cylinder, and TLCvisualizer (CAMAG, Switzerland), balance (Sartorius, Germany), and thin layer silica gel preformed plate (Merck, Germany).

[0036] Experimental reagents and test drugs:

[0037] Reagents: Toluene, ethyl acetate, methanol, sulfuric acid, and ethyl acetate were all analytical grade (Guangzhou Chemical Reagent Factory); water was purified water prepared in the laboratory.

[0038] The preparation method of 10% sulfuric acid ethanol solution is as follows: slowly pour 10 ml of concentrated sulfuric acid into 90 ml of ethanol solution and let it cool.

[0039] The sources of the peach kernel and bitter almond 1-11 medicinal materials involved in Example 1 of the present invention are:

[0040] 1-7: All from Anhui Keyun Baicao Pharmaceutical Co., Ltd., the batch numbers are: TR2409001, TR 2409002, TR2409003, TR 2409004, TR 2409005, TR 2409006, TR 2409007.

[0041] 8-11: All are from Anhui Keyun Baicao Pharmaceutical Co., Ltd., and the batch numbers are: KXR 2409001, KXR2409002, KXR 2409003, and KXR 2409004.

[0042] The peach kernels and bitter almonds 1-8 involved in Example 2 of the present invention are all from Anhui Keyun Baicao Pharmaceutical Co., Ltd., the batch number of the peach kernels is TR 2409003, and the batch number of the bitter almonds is KXR 2409004.

[0043] Example 1

[0044] This embodiment provides a thin layer identification method for peach kernels and bitter almonds, comprising the following steps:

[0045] 1. Sample Preparation

[0046] Preparation of test solution: Take appropriate amount of peach kernel medicinal materials 1-7 and bitter almond medicinal materials 8-11, grind them into powder, take 1g, add 50ml of 70% by volume ethanol, ultrasonically treat (power 250W, frequency 40kHz) for 30 minutes, filter through medium-speed double-circle qualitative filter paper (pore size 30-50 microns), evaporate to dryness, add 2ml of 70% by volume ethanol to dissolve, and obtain peach kernel and bitter almond solutions.

[0047] 2. Chromatographic conditions

[0048] Thin layer plate: prefabricated silica gel ordinary thin layer plate (20cm×10cm; Merck)

[0049] Spotting: 5μL, spot on the thin layer plate in strips, with a strip width of 10mm. The distance between the spotting origin and the lower edge of the thin layer plate is 10mm. After spotting, heat on a thin layer heating plate at 45℃ for 15 minutes and set aside.

[0050] Developing solvent: toluene-ethyl acetate-methanol (volume ratio is 15:2:2)

[0051] Development conditions: T: 24°C, RH: 79%

[0052] Inspection: Spray with 10% sulfuric acid ethanol solution, heat at 105°C for 3 min, observe under ultraviolet light (366 nm) and take images.

[0053] The results are as follows Figure 1 As shown in the figure, there is a blue characteristic spot at the position of Rf≈0.28 in the spectrum of bitter almonds, and there is no interference from other spots at this position in the spectrum of peach kernels, indicating that the method has good specificity and applicability.

[0054] Example 2

[0055] Different proportions of bitter almonds were added to peach kernel medicinal materials, as shown below: 1: peach kernel medicinal materials; 2: mixed medicinal materials consisting of peach kernel medicinal materials (90% by weight) and bitter almond medicinal materials (10% by weight); 3: mixed medicinal materials consisting of peach kernel medicinal materials (80% by weight) and bitter almond medicinal materials (20% by weight); 4: mixed medicinal materials consisting of peach kernel medicinal materials (70% by weight) and bitter almond medicinal materials (30% by weight); 5: mixed medicinal materials consisting of peach kernel medicinal materials (60% by weight) and bitter almond medicinal materials (40% by weight); 6: mixed medicinal materials consisting of peach kernel medicinal materials (50% by weight) and bitter almond medicinal materials (50% by weight); 7: mixed medicinal materials consisting of peach kernel medicinal materials (40% by weight) and bitter almond medicinal materials (60% by weight); 8: bitter almond medicinal materials. The results of the test by the method of Example 1 are as follows: Figure 2 As shown in the figure, when 10% bitter almonds are mixed into peach kernels, the characteristic spots of bitter almonds can also be detected, which proves that this method is very practical.

[0056] The following comparative examples are used for illustration. In fact, the applicant used many kinds of developing agents and different proportions during the experiment, which are not limited to the following comparative examples. The comparative examples are just examples of a few.

[0057] Comparative Example 1

[0058] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was petroleum ether II-chloroform-acetone, with a volume ratio of 5:15:0.3. The results were as follows Figure 3 As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006), 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009). Conclusion: The spectrum of this method shows fewer spots, and these spots are all common components of peach kernels and bitter almonds, and peach kernels and bitter almonds cannot be identified.

[0059] Comparative Example 2

[0060] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-ethyl acetate-anhydrous ethanol, with a volume ratio of 15:1:4. Figure 4As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows more spots, and peach kernel has spots at the RF value where the characteristic spots of bitter almond are located, which will cause interference and affect the identification of peach kernel and bitter almond.

[0061] Comparative Example 3

[0062] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-butyl acetate-methanol, with a volume ratio of 15:1:4. Figure 5 As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows more and more concentrated spots, and the separation is poor. Peach kernel has spots at the RF value where the characteristic spots of bitter almond are located, which will cause interference and affect the identification of peach kernel and bitter almond.

[0063] Comparative Example 4

[0064] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-isopropanol-methanol, with a volume ratio of 15:1:4. Figure 6 As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows many spots and they are all concentrated in the middle, with poor separation. Peach kernel has spots near the RF value of the bitter almond characteristic spots, which will cause interference and affect the identification of peach kernel and bitter almond.

[0065] Comparative Example 5

[0066] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-butyl acetate-anhydrous ethanol, with a volume ratio of 15:1:4. Figure 7 As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows fewer spots, the logarithmic spots are overlapped, and the bitter almond characteristic spots are also overlapped with other spots, and it is impossible to distinguish peach kernels and bitter almonds.

[0067] Comparative Example 6

[0068] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-ethyl acetate-methanol, with a volume ratio of 15:3:3. Figure 8 As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows more spots, and most of the spots are relatively diffuse. The characteristic spots of bitter almonds also have obvious diffusion. If the spots are slightly weak, it is easy to misjudge that there are no characteristic spots. It is not conducive to the identification of peach kernels and bitter almonds.

[0069] Comparative Example 7

[0070] The thin layer identification method of peach kernel and bitter almond in Example 1 was used, except that the developing solvent used was toluene-ethyl acetate-methanol, with a volume ratio of 15:3:2. Fig. 9As shown. Among them, 1. Bitter almond control extract (Guangzhou Keman Biotechnology Co., Ltd.: EKXR-20241201); 2-3. Bitter almond medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: KXR 2409005, KXR 2409006); 4: Peach kernel control extract (Guangzhou Keman Biotechnology Co., Ltd.: ETR-20241201); 5-6: Peach kernel medicinal materials (Anhui Keyun Baicao Pharmaceutical Co., Ltd., batch numbers are: TR2409008, TR2409009) Conclusion: The spectrum of this method shows more spots, and the separation degree of bitter almond characteristic spots is not good, which is easy to overlap with the spots below, affecting the identification of peach kernels and bitter almonds.

[0071] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A thin layer identification method for peach kernels and bitter almonds, characterized in that: The steps include: Take peach kernel and bitter almond to prepare peach kernel and bitter almond solution; Peach kernel and bitter almond solution were aspirated and tested by TLC method; The developing solvents used in the thin layer identification method are toluene, ethyl acetate and methanol.

2. The thin layer identification method of peach kernel and bitter almond according to claim 1, characterized in that: The volume ratio of toluene, ethyl acetate and methanol in the developing solvent is (13-17):(1-3):(1-3).

3. The thin layer identification method of peach kernel and bitter almond according to claim 2, characterized in that: The volume ratio of toluene, ethyl acetate and methanol in the developing solvent is 15:2:

2.

4. The thin layer identification method of peach kernel and bitter almond according to claim 1, characterized in that: The preparation method of the peach kernel and bitter almond solution is as follows: respectively taking peach kernel and bitter almond medicinal material powders, respectively adding ethanol, ultrasonicating, filtering, evaporating to dryness, and adding ethanol to dissolve to obtain the peach kernel and bitter almond solution.

5. The thin layer identification method of peach kernel and bitter almond according to claim 1, characterized in that: The volume fraction of the ethanol added for the first time is 65-75%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material powder to the ethanol added for the first time is 1 / 45-55, in g / ml; Preferably, the volume fraction of the ethanol added for the first time is 70%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material to the ethanol added for the first time is 1 / 50, in units of g / ml.

6. The thin layer identification method of peach kernel and bitter almond according to claim 5, characterized in that: The volume fraction of the ethanol added for the second time is 65-75%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material powder to the ethanol added for the second time is 1 / 1-3, in g / ml; Preferably, the volume fraction of the ethanol added for the first time is 70%, and the weight-to-volume ratio of the peach kernel or bitter almond medicinal material to the ethanol added for the second time is 1 / 2, in units of g / ml.

7. The thin layer identification method of peach kernel and bitter almond according to claim 4, characterized in that: The power of the ultrasound is 250W, the frequency is 40kHz, the ultrasound time is 30 minutes, and the filtration is performed using medium-speed double-circle qualitative filter paper, that is, the pore size is 30 to 50 microns.

8. The thin layer identification method of peach kernel and bitter almond according to claim 1, characterized in that: The thin layer identification method is as follows: spotting peach kernel and bitter almond solution on a thin layer plate, developing with a developing agent, taking out, drying, and inspecting under ultraviolet light; Preferably, the thin layer plate is a common thin layer plate made of silica gel.

9. The thin layer identification method of peach kernel and bitter almond according to claim 8, characterized in that: The weight-to-volume ratio of the peach kernel or bitter almond medicinal material powder to the sample amount is 1 / 4-6, and the unit is g / μL. The sample is spotted on the thin layer plate in a strip shape, the strip width is 9-11 mm, and the distance between the spotting origin and the lower edge of the thin layer plate is 9-11 mm. After spotting, the sample is placed on a thin layer heating plate at 40-50° C. and heated for 10-20 min. Preferably, the weight-to-volume ratio of the peach kernel or bitter almond medicinal powder to the spotting amount is 1 / 5, expressed in g / μL. The sample is spotted on the thin layer plate in a strip shape, the strip width is 10 mm, the distance between the spotting origin and the lower edge of the thin layer plate is 10 mm, and after spotting, the sample is heated on a thin layer heating plate at 45°C for 15 min.

10. The thin layer identification method of peach kernel and bitter almond according to claim 8, characterized in that: The color developer used in the thin layer identification method is 10% sulfuric acid ethanol solution, the heating is heating at 105° C. for 3 minutes, and the wavelength of the inspection ultraviolet light is 366 nm.

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