Combustion-based metabolite detection device and method

Abstract

The application discloses a combustion mode-based metabolite testing device and method. The metabolite testing device comprises a combustion control module, a respiratory gas flow control module, an exhaled gas heating and humidifying control module, a respiratory gas separation module, a control unit and a computer. The combustion control module is used for simulating human exhaled gas; the respiratory gas flow control module is used for simulating human respiratory flow; the exhaled gas heating and humidifying control module is used for simulating the temperature and humidity state of human exhaled gas; and the respiratory gas separation module is used for simulating the human mouth and nose breathing process. The control unit is responsible for the control of motors and valves, and the computer is responsible for data acquisition, calculation analysis and result display. The testing method is to set human metabolic parameters, connect a human metabolic tester and test, and finally perform quality inspection analysis on the metabolic parameters of the human metabolic tester according to the test result. The application can be applied to the application scene of human metabolic tester accuracy evaluation, and realizes easy, economical and effective verification of the human metabolic tester.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of energy metabolism, in particular to a metabolic quality detection device and method based on combustion mode. BACKGROUND

[0002] Metabolic-related diseases such as diabetes, uremia, hypercalcemia, and metabolic syndrome are closely related to unhealthy eating habits, lack of exercise, and genetic factors. Human metabolic detectors play a crucial role in medical applications such as nutrition support, prevention and diagnosis of metabolic diseases, and provide important evidence for doctors to develop personalized nutrition support programs with accurate metabolic test results. Therefore, to provide solid data support for clinical diagnosis, the effectiveness of human metabolic detector indicators is crucial to doctors. That is, the metabolic indicators of the human metabolic detector need to be subjected to essential quality detection means to ensure the accuracy and reliability of the results issued by the detector.

[0003] Currently, the quality detection of human metabolic detectors mainly uses standard gas ventilation quality detection method. According to patent document CN108133653A, a simulation method and device for human lung gas exchange are introduced. The device simulates the lung respiratory function of the human body by controlling the movement of the cylinder piston, and then realizes the quantitative release of carbon dioxide with a specific concentration in the cylinder to simulate the gas metabolism process of the human body. This method can change the output of the simulation by adjusting the concentration parameters of the standard gas, thereby adapting to different gas metabolism rates. However, this method also has limitations. On the one hand, the standard gas ventilation technology makes the cost of the equipment higher, and the operation process is complex, which requires precise control of the gas composition and flow. On the other hand, the system cannot effectively simulate the temperature and humidity of the human exhaled gas, limiting its applicability in real physiological conditions, resulting in a significant deviation between the experimental results and the actual human metabolic process, thereby reducing the accuracy and reliability of the research. SUMMARY

[0004] To solve the above technical problems, the present application proposes a metabolic quality detection device and method based on combustion mode, which is suitable for the application scenario of verifying the accuracy of human metabolic testers, and can realize controllable, easy, and economical effectiveness verification of human metabolic detectors.

[0005] In view of the above, one of the objectives of the present application is to provide a metabolic quality detection device based on combustion mode. The specific description is as follows:

[0006] A metabolic quality control device based on combustion is disclosed, comprising a combustion control module, a respiratory gas flow control module, an exhaled gas heating and humidification control module, a respiratory gas separation module, a control unit, and a computer. The combustion control module generates gas with relatively low oxygen concentration and relatively high carbon dioxide concentration, simulating human exhalation. The respiratory gas flow control module draws air from the combustion control module and exhausts air to the exhaled gas heating and humidification control module, generating a controllable respiratory gas flow rate, simulating human respiratory flow. The exhaled gas heating and humidification control module provides constant temperature humidification to the air exhausted from the respiratory gas flow control module, simulating the temperature and humidity of human exhalation. The respiratory gas separation module separates the simulated exhaled air exhausted from the exhaled gas heating and humidification control module from the simulated inhaled air delivered to the combustion control module, simulating the human mouth and nose breathing process. The control unit controls various motors and valves within the device. The computer is responsible for data collection, calculation and analysis, human-computer interaction, and result display.

[0007] The second objective of this invention is to provide a metabolic quality control method based on combustion. Based on the aforementioned metabolic quality control device based on combustion, the specific quality control operation steps are described as follows:

[0008] Step 1: Set the simulated human metabolic respiratory quotient RQ, metabolic rate EE, and human respiratory rate RR; specifically, determine the RQ to be simulated by selecting the type of combustion liquid substrate, determine the EE by the syringe plunger propulsion speed, and determine the RR by controlling the reciprocating motion speed of the piston cylinder.

[0009] Step 2: Connect and install the human metabolic analyzer to be tested to this device, start the metabolic test function of the human metabolic analyzer to be tested, and begin the test;

[0010] Step 3: Quality control results analysis of the tested human metabolic analyzer. First, record the human metabolic respiratory quotient from the test results of the human metabolic analyzer. metabolic rate and human breathing rate Then, record the simulated respiratory quotient (RQ), metabolic rate (EE), and respiratory rate (RR); finally, calculate the relative error between them using the following formula:

[0011] (14)

[0012] (15)

[0013] (16)

[0014] in, This represents the relative error of the human metabolic respiratory quotient. This represents the relative error in metabolic rate consumption. The relative error of the human respiratory frequency.

[0015] The present application has the following advantages:

[0016] 1. The present application uses combustion method and exhalation heating and humidification control to realize the simulation of human exhalation and avoid the use of standard gas consumables, providing a more similar human respiratory, economical and simulated human respiratory quality inspection device.

[0017] 2. The present application simulates human oral and nasal respiration based on a respiratory gas separation module, supports the quality inspection test application of two kinds of metabolic instruments, namely head cover type metabolic detector and mouth-to-mouth gas type metabolic detector, that is, the simulation of human metabolism and respiration can be realized by placing the simulated human respiratory gas port in the head cover or installing the respiratory mask pipeline.

[0018] 3. The present application can generate adjustable, wide-range carbon dioxide production per minute (VCO2) and oxygen uptake per minute (VO2) reference values by controlling the delivery rate of the combustion liquid substrate. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 is a structural schematic diagram of a metabolic quality inspection device based on the combustion method of the present application;

[0020] Figure 2 is a structural schematic diagram of a respiratory gas flow control module of the present application;

[0021] Figure 3 is a flowchart of a metabolic quality inspection method based on the combustion method of the present application.

[0022] Among them, the reference signs are: 1-computer, 2-control unit, 3-second motor, 4-single-channel linear slide rail, 5-syringe, 6-fireproof rack, 7-heat insulation plate, 8-lamp wick, 9-infusion tube, 10-combustion chamber, 15-Meng's washing bottle, 16-electric heating constant temperature water tank, 17-first one-way valve, 18-second one-way valve, 19-simulated human respiratory gas port, 2.1-gear set, 2.2-crank connecting rod mechanism, 2.3-piston cylinder, 2.4-first motor. DETAILED DESCRIPTION

[0023] For the purpose, technical solution and advantages of the present application, the embodiments of the present application will be further described below in conjunction with the drawings.

[0024] Reference Figure 1 ​​The embodiment provides a combustion mode-based metabolite detection device, which comprises a respiratory gas flow control module, a combustion control module, an exhaled gas heating and humidifying control module, a respiratory gas separation module, a control unit 2 and a computer 1. The combustion control module is used for simulating human exhaled gas; the respiratory gas flow control module is used for simulating human respiratory flow; the exhaled gas heating and humidifying control module is used for simulating the temperature and humidity state of human exhaled gas; the respiratory gas separation module is used for simulating the human oral-nasal breathing process; the control unit 2 is responsible for the control of motors and valves in the device and signal acquisition; and the computer 1 is used for data acquisition, calculation analysis, human-computer interaction and result display.

[0025] The combustion control module is composed of a second motor 3, a single-channel linear slide rail 4, a syringe 5, a transfusion tube 9, a combustion chamber 10, a wick 8, a heat insulation plate 7, a fireproof stand 6 and a gas pipe. Further, under the driving of the second motor 3, the ball screw in the single-channel linear slide rail 4 performs linear motion, the syringe 5 core rod is pushed, the combustion liquid substrate in the syringe 5 is injected to the wick 8 through the transfusion tube 9 via the syringe 5 cone head, and the control of the combustion rate of the combustion liquid substrate by the second motor 3 is realized. At the same time, the simulated inhaled gas is introduced into the combustion chamber 10 via the second one-way valve 18, the combustion of the combustion liquid substrate is performed, the simulated exhaled gas with relatively low oxygen concentration and relatively high carbon dioxide concentration is generated, and the simulated exhaled gas is discharged to the respiratory gas flow control module, so that the simulated human exhaled gas is realized. Preferably, the combustion liquid substrate is an ethanol solution with a concentration of 99.9%; the transmission ratio of the single-channel linear slide rail 4 is 1:1; the syringe 5 has a specification of 20ml and a cross-sectional area of 2cm 2 .

[0026] Referring to Figure 2The breathing gas flow control module is composed of a first motor 2.4, a gear set 2.1, a crank connecting rod mechanism 2.2, a piston cylinder 2.3 and a gas pipe. Further, first, the first motor 2.4 is connected to drive the crank connecting rod mechanism 2.2 after speed change through the gear set 2.1; then, the rotary motion of the first motor 2.4 is converted into the linear reciprocating motion of the piston rod of the piston cylinder 2.3 through the crank connecting rod mechanism 2.2; finally, the frequency adjustment of the linear reciprocating motion of the piston cylinder 2.3 controlled by the first motor 2.4 is realized. During the simulation of the inhalation process, the piston rod of the piston cylinder 2.3 moves from the top end to the bottom end, and the piston cylinder 2.3 extracts the simulated exhaled gas in the combustion control module; during the simulation of the exhalation process, the piston rod of the piston cylinder 2.3 moves from the bottom end to the top end, and the piston cylinder 2.3 discharges the simulated exhaled gas to the exhalation heating and humidification control module. Then, the control of the gas in / out flow of the piston cylinder 2.3 can be realized by adjusting the rotating speed of the first motor 2.4, the cycle process of the extraction of the combustion control module and the discharge to the exhalation heating and humidification control module is realized synchronously with the human body breathing frequency, and the human body breathing flow is simulated. Preferably, the gear set 2.1 has a gear ratio of 2, the gear ratio of the crank connecting rod mechanism 2.2 is 1, and the rotating speed of the first motor 2.4 is 30 revolutions per minute.

[0027] The exhalation heating and humidification control module is composed of a Meng's washing bottle 15, an electric heating constant temperature water tank 16 and a gas pipe. Further, the Meng's washing bottle 15 containing saturated carbonic acid solution is installed in the constant temperature water tank for water bath heating. Then, when the simulated exhaled gas is discharged from the breathing gas flow control module to the gas inlet of the Meng's washing bottle 15, the simulated exhaled gas is discharged from the gas outlet of the Meng's washing bottle 15 after passing through the constant temperature saturated carbonic acid solution, so that the simulated exhaled gas discharged from the breathing gas flow control module is subjected to constant temperature humidification treatment, and the temperature and humidity state of the human body exhaled gas is simulated. Preferably, the temperature of the electric heating constant temperature water bath is set to 37℃, and sodium bicarbonate is added to the solution in the Meng's washing bottle 15 to form a saturated carbonic acid solution.

[0028] The breathing gas separation module is composed of a first one-way valve 17, a second one-way valve 18, a simulated human body breathing gas port 19 and a gas pipe. Further, when the simulated exhaled gas in the exhalation heating and humidification control module flows to the simulated human body breathing gas port 19 through the first one-way valve 17, the simulated exhaled gas is discharged to the human body metabolism detector during the simulation of the exhalation; when the inhaled gas flows to the combustion chamber 10 in the combustion control module through the simulated human body breathing gas port 19 and the second one-way valve 18 in sequence during the simulation of the inhalation, the simulated exhaled gas discharged from the exhalation heating and humidification control module is separated from the simulated inhaled gas transported to the combustion control module, and the human body mouth and nose breathing process is simulated.

[0029] Reference is made to Figure 3The second object of the present application is to provide a combustion-based metabolic substance detection method. Based on the above-mentioned detection device, the specific steps are described as follows:

[0030] Step 1: Set the respiratory quotient (RQ) of the human body metabolism to be simulated, the metabolic rate (EE) and the respiratory rate (RR) of the human body. The calculation method of RQ, EE and RR is further explained as follows:

[0031] The combustion liquid substrate is an ethanol solution with a concentration of 99.9%. The respiratory quotient RQ of the human body metabolism to be simulated is set to 0.67. The chemical formula of ethanol combustion is as shown in formula (1):

[0032] (1)

[0033] wherein x represents the number of carbon atoms contained in the hydrocarbon group; y represents the number of hydrogen atoms contained in the hydrocarbon group;

[0034] Then, the expression of the set RQ to be simulated is:

[0035] (2)

[0036] The metabolic rate (EE) is calculated based on the weir formula (2), combined with the atomic composition of ethanol and the advancing speed of the 5-core rod of the syringe . The expression of the EE formula derivation is as follows:

[0037] (3)

[0038] (4)

[0039] (5)

[0040] (6)

[0041] (7)

[0042] (8)

[0043] wherein EE is the metabolic rate (kcal / day); is the oxygen uptake rate ; is the carbon dioxide production rate per minute ; is the amount of substance of oxygen uptake per minute ; is the amount of substance of carbon dioxide production per minute ; is the amount of substance of ethanol consumption per minute ; Molecular weight of ethanol ; Mass of combusted liquid substrate consumed per minute ; Cross-sectional area of syringe 5 barrel ; Density of ethanol (0.7893 g / ml) Syringe 5 plunger advance speed ; Molar volume of an ideal gas .

[0044] Combining equations (2) (7), the expression for EE is:

[0045] (9)

[0046] Combining the first motor 2.4 rpm, the gear set 2.1 ratio, and the crank linkage 2.2 ratio, the RR formula derivation is expressed as follows:

[0047] (10)

[0048] (11)

[0049] (12)

[0050] Where, First motor 2.4 rpm (r / min) Speed after gear set 2.1 (r / min) Piston cylinder 2.3 reciprocating frequency (times / min) Gear set 2.1 ratio Crank linkage 2.2 ratio; RR is respiratory rate (times / min)

[0051] Combining equations (9) (10), the expression for RR is:

[0052] (13)

[0053] Step two: Connect and install the human body metabolism detector to the device, start the human body metabolism detector metabolism test function, and begin testing.

[0054] In the headgear human metabolism test application, the simulated human respiratory gas port 19 is placed in the headgear to simulate human metabolism and respiration. In the mouth-to-mouth human metabolism test application, the simulated human respiratory gas port 19 is connected in the breathing mask pipeline through the trachea to simulate human metabolism and respiration. Specifically, during simulated human inhalation, first, the piston rod of the piston cylinder 2.3 moves from the top end to the bottom end to pump the gas in the headgear or breathing mask pipeline; then, the gas is pumped to the combustion chamber 10 inlet through the second one-way valve 18, and an oxidation combustion reaction occurs in the combustion chamber 10 with the combustion liquid substrate. During this period, the second motor 3 adjusts the injection rate of the combustion liquid substrate to control the flame size and achieve the simulation of the set metabolic rate; finally, the simulated exhaled gas generated by combustion is pumped into the piston cylinder 2.3. During simulated human exhalation, first, the piston rod of the piston cylinder 2.3 moves from the bottom end to the top end to discharge the simulated exhaled gas in the piston cylinder 2.3 to the Meng's washing bottle 15 of the exhalation heating and humidification control module; then, after the simulated exhaled gas is heated by the constant-temperature saturated carbonic acid solution in the Meng's washing bottle 15 and mixed with saturated water vapor, it is discharged through the first one-way valve 17 through the simulated human respiratory gas port 19; finally, it returns to the headgear or mask pipeline to complete the simulation of one human respiratory process. The cycle is repeated until the quality inspection test time is reached, and the quality inspection test is completed.

[0055] Step three: analysis of the quality inspection results of the human metabolism detector under test. First, record the respiratory quotient , metabolic rate , and human respiratory frequency of the human metabolism detector test results; then, record the set simulated respiratory quotient , metabolic rate , and human respiratory frequency ; finally, calculate the corresponding relative errors between them, and the calculation formula is as follows:

[0056] (14)

[0057] (15)

[0058] (16)

[0059] Among them, is the relative error of the respiratory quotient of human metabolism; is the relative error of the metabolic rate consumption; is the relative error of the human respiratory frequency.

[0060] The above only describes the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, etc. made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A metabolic quality control method based on combustion, characterized in that, The method uses a combustion-based metabolic quality control device, which includes a combustion control module, a respiratory gas flow control module, an exhaled gas heating and humidification control module, a respiratory gas separation module, a control unit, and a computer. The combustion control module is used to simulate human exhaled air; The respiratory air flow control module is used to simulate human respiratory flow. The exhalation heating and humidification control module is used to simulate the temperature and humidity state of human exhaled air; The respiratory gas separation module is used to simulate the human mouth and nose breathing process; The control unit is responsible for controlling the motors and valves in the device and acquiring signals. The computer is used for data acquisition, calculation and analysis, human-computer interaction, and result display; The combustion control module consists of a second motor (3), a single-channel linear slide rail (4), a syringe (5), an infusion tube (9), a combustion chamber (10), a lamp wick (8), a heat insulation plate (7), a fireproof frame (6), and an air pipe. The fireproof frame (6) is placed inside the combustion chamber (10), and a heat insulation plate (7) is provided on its top. The second motor (3) is connected to the ball screw of the single-channel linear slide rail (4) through a coupling. The linear rail of the single-channel linear slide rail (4) is connected to the core rod of the syringe (5). The cone of the syringe (5) is installed inside the infusion tube (9). A lamp wick (8) is installed at the end of the infusion tube (9), and the lamp wick (8) is located at the center position below the heat insulation plate (7). The air inlet of the combustion chamber (10) is connected to the breathing gas separation module through an air pipe, and the air outlet of the combustion chamber (10) is connected to the breathing gas flow control module through an air pipe. The breathing air flow control module consists of a first electric motor (2.4), a gear set (2.1), a crank-connecting rod mechanism (2.2), a piston cylinder (2.3), and an air pipe; the first electric motor (2.4) is connected in sequence to the piston rod of the gear set (2.1), the crank-connecting rod mechanism (2.2), and the piston cylinder (2.3), so that the first electric motor (2.4) drives the piston of the piston cylinder (2.3) to reciprocate linearly. The quality inspection method includes the following steps: Step 1: Set the simulated human metabolic respiratory quotient metabolic rate and human breathing rate Specifically, the type of combustion liquid substrate is selected to determine the substance to be simulated. EE is determined by the push speed of the syringe (5) core rod, and RR is determined by controlling the reciprocating speed of the piston cylinder (2.3). Step 2: Connect and install the human metabolic analyzer to be tested to this device, start the metabolic test function of the human metabolic analyzer to be tested, and begin the test; Step 3: Quality control results analysis of the tested human metabolic analyzer; First, record the human metabolic respiratory quotient from the test results of the human metabolic analyzer. metabolic rate and human breathing rate Then, record the simulated respiratory quotient. metabolic rate and human breathing rate Finally, calculate the relative error between them, using the following formula: （14） （15） （16） in, This represents the relative error of the human metabolic respiratory quotient. This represents the relative error in metabolic rate consumption. This represents the relative error of human respiratory rate; metabolic rate The expressions include: （9） in, The molecular weight of the liquid substrate being burned; The cross-sectional area of ​​the syringe (5) tube diameter; The density of the liquid substrate being burned; The speed at which the syringe (5) core rod advances; For an ideal gas molar volume; Indicates the number of carbon atoms in a hydrocarbon group; This indicates the number of hydrogen atoms contained in the hydrocarbon group.

2. The metabolic quality control method based on combustion as described in claim 1, characterized in that, In step one, The general chemical formula of the liquid substrate for combustion is , Represented as a hydrocarbon group, i.e., a chain or ring structure composed of hydrogen and carbon, the general formula for this combustion liquid substrate is: ,Right now The chemical formula for the oxidative combustion of a liquid substrate is as follows: : （1） So, let's set the simulation target. The expressions include: （2） based on formula Calculate metabolic rate ( ), combining the atomic composition of the burning liquid substrate and the propulsion speed of the syringe (5) core rod; The formula derivation is expressed as follows: （3） （4） （5） （6） （7） （8） in, Metabolic rate oxygen uptake rate The rate of carbon dioxide production per minute; The amount of oxygen consumed per minute; The amount of carbon dioxide produced per minute; The amount of liquid substrate consumed per minute; The molecular weight of the liquid substrate being burned; The mass of liquid substrate consumed per minute; The cross-sectional area of ​​the syringe (5) tube diameter; The density of the liquid substrate being burned; The speed at which the syringe (5) core rod advances; molar volume of an ideal gas Combining the speed of the first electric motor (2.4), the transmission ratio of the gear set (2.1), and the transmission ratio of the crank-connecting rod mechanism (2.2), The formula derivation is expressed as follows: （10） （11） （12） in, The rotational speed of the first motor (2.4); The rotational speed after passing through the gear set (2.1); The reciprocating frequency of the piston cylinder (2.3); The transmission ratio of the gear set (2.1); The transmission ratio of the crank-connecting rod mechanism (2.2); Respiratory rate; Combined , The expressions include: （13）。

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