Microfluidic device and microfluidic detection device

Abstract

A microfluidic device (110) and a microfluidic detection device (1000), the microfluidic device (100) comprising a body (110) and a flow channel switching assembly (120), the body (110) being provided with a plurality of flow channels, the flow channel switching assembly (120) being rotatably connected to the body (110), the flow channel switching assembly (120) being provided with a liquid passing groove, and the flow channel switching assembly (120) being arranged to be capable of rotating relative to the body (110), the position of the liquid passing groove is adjusted so that at least two of the multiple flow channels can be communicated through the liquid passing groove. The microfluidic detection device (1000) comprises a sensing device (200) and a microfluidic device (100).

Description

Microfluidic device and microfluidic detection device Technical Field

[0001] The present invention belongs to the field of microfluid control technology, in particular to a microfluidic device and a microfluidic detection device. Background Art

[0002] Microfluidics, the technology for controlling, manipulating, and detecting complex fluids at microscopic scales, is a new interdisciplinary field developed from the foundations of microelectronics, micromechanics, bioengineering, and nanotechnology. Experiments in biology, chemistry, materials science, and other fields frequently require manipulation of fluids. For example, DNA sample preparation, liquid chromatography, PCR reactions, and electrophoresis detection are all performed in liquid environments. If sample preparation, biochemical reactions, and result detection are integrated onto a biochip, the volume of fluid used in the experiment must be reduced from milliliters to microliters, making powerful microfluidic devices indispensable. Microfluidics have a wide range of applications in biotechnology research due to their compact size, low sample / reagent requirements, fast reaction speed, massively parallel processing, and disposable design.

[0003] However, existing microfluidic devices can generally only control the on / off of a single flow channel. To achieve the switching and on / off of multiple flow channels, multiple control valves are generally used, which makes the structure of the microfluidic device complex and the cost high.

[0004] Summary of the Invention

[0005] The present invention provides a microfluidic device and a microfluidic detection device to solve the technical problem in the prior art that the existing microfluidic device adopts an overly complex multi-valve structure to achieve control of multi-channel switching and on-off.

[0006] To solve the above problems, the present invention adopts the following solutions:

[0007] In a first aspect, the present invention provides a microfluidic device, which includes a main body and a flow channel switching assembly; the main body is provided with multiple flow channels; the flow channel switching assembly is rotatably connected to the main body, the flow channel switching assembly is provided with a liquid flow channel, and the flow channel switching assembly is configured to be rotatable relative to the main body to adjust the position of the liquid flow channel so that at least two of the multiple flow channels are connected through the liquid flow channel.

[0008] In an optional scheme of the present invention, the multiple flow channels include a first flow channel, a second flow channel and a third flow channel; the liquid flow trough includes a first liquid flow trough, a second liquid flow trough and a third liquid flow trough; the flow channel switching component is configured to rotate to a first position, and the first liquid flow trough connects the first flow channel and the second flow channel; the flow channel switching component is configured to rotate to a second position, and the second liquid flow trough connects the second flow channel and the third flow channel, and the third liquid flow trough connects the first flow channel and the second flow channel.

[0009] In an optional solution of the present invention, the multiple flow channels further include a fourth flow channel; and the flow channel switching assembly is further configured so that when it is rotated to a third position, the second liquid transfer trough connects the third flow channel and the fourth flow channel.

[0010] In an optional scheme of the present invention, the first flow channel includes a second flow channel opening, the second flow channel includes a third flow channel opening and a fourth flow channel opening, the third flow channel includes a fifth flow channel opening and a sixth flow channel opening, and the second flow channel opening, the third flow channel opening, the fourth flow channel opening, the fifth flow channel opening and the sixth flow channel opening are arranged overlapping with the flow channel switching component in the upper and lower directions.

[0011] In an optional solution of the present invention, the first flow channel also includes a first flow channel opening, which is located on one side of the flow channel switching component; the microfluidic device also includes a fluid storage component, which is connected to the body and communicates with the first flow channel opening.

[0012] In an optional solution of the present invention, the third flow channel further includes a seventh flow channel opening, which is located on one side of the flow channel switching assembly and is used to discharge the fluid in the third flow channel.

[0013] In an optional solution of the present invention, the fourth flow channel includes an eighth flow channel opening and a ninth flow channel opening, and the eighth flow channel opening and the flow channel switching assembly are arranged to overlap in the upper and lower directions.

[0014] In an optional solution of the present invention, the third flow channel is a flow channel that extends in a circuitous manner and is used to collect fluid.

[0015] In an optional solution of the present invention, the main body is provided with a slide groove; the flow channel switching assembly includes a limiting component, which is configured to slide in the slide groove during the rotation of the flow channel switching assembly relative to the main body to enable the flow channel switching assembly to switch between the first position, the second position and the third position.

[0016] In an optional solution of the present invention, the chute includes a first chute section, a second chute section and a third chute section, and the limiting component is configured to slide along the first chute section to rotate the flow channel switching assembly to the first position, slide along the second chute section to rotate the flow channel switching assembly to the third position, and slide along the third chute section to rotate the flow channel switching assembly to the second position.

[0017] In an optional solution of the present invention, the chute is configured so that when the limiting component is located in the second chute section or the third chute section, it cannot slide to the first chute section.

[0018] In an optional solution of the present invention, the groove depth of the second chute section is equal to the groove depth of the third chute section, and the groove depths of the second chute section and the third chute section are greater than the groove depth of the first chute section.

[0019] In an optional solution of the present invention, the flow channel switching assembly also includes a rotating body and a sealing plate. The rotating body is rotatably connected to the body. The sealing plate and the limiting component are both connected to the rotating body and can rotate with the rotating body. The sealing plate is arranged in contact with the body; the first liquid flow trough, the second liquid flow trough and the third liquid flow trough are arranged on the sealing plate.

[0020] In an optional solution of the present invention, the main body includes an upper plate, a flow channel plate and a lower plate, and the upper and lower surfaces of the flow channel plate are provided with flow channel grooves; the upper plate and the lower plate are respectively arranged on the upper and lower sides of the flow channel plate to form multiple flow channels together with the flow channel grooves, and the multiple flow channels include a second flow channel.

[0021] In an optional scheme of the present invention, the multiple flow channels also include a first flow channel and a fourth flow channel; the flow channel groove includes a first flow channel groove and a fourth flow channel groove, and the first flow channel groove and the fourth flow channel groove are arranged on the upper surface of the flow channel plate; the first flow channel groove and the upper plate together form a first flow channel, and the fourth flow channel groove and the upper plate together form a fourth flow channel.

[0022] In an optional scheme of the present invention, the multiple flow channels also include a third flow channel; the flow channel includes a third flow channel, and the third flow channel includes a first section of the third flow channel, a second section of the third flow channel and a third section of the third flow channel; the first section of the third flow channel and the third section of the third flow channel are arranged on the upper surface of the flow plate, and the second section of the third flow channel is arranged on the lower surface of the flow plate; the first section of the third flow channel, the second section of the third flow channel and the third section of the third flow channel are connected in sequence and form a third flow channel together with the upper plate and the lower plate.

[0023] In an optional scheme of the present invention, the second flow channel includes a first section of the second flow channel and a second section of the second flow channel; the flow channel includes a second flow channel, the second flow channel includes a first section of the second flow channel and a second section of the second flow channel, the first section of the second flow channel and the second section of the second flow channel are arranged on the upper surface of the flow channel plate; the upper surface of the lower plate is provided with a first buffer groove and a second buffer groove; the first section of the second flow channel is connected to the first buffer groove, and together with the upper plate and the lower plate forms the first section of the second flow channel; the second section of the second flow channel is connected to the second buffer groove, and together with the upper plate and the lower plate forms the second section of the second flow channel; the first buffer groove is provided with a first liquid port, the second buffer groove is provided with a second liquid port, and the first section of the second flow channel and the second section of the second flow channel can be connected through the first liquid port and the second liquid port.

[0024] In an optional solution of the present invention, the main body is provided with a transparent observation area, and the observation area is located above the second flow channel.

[0025] In an optional solution of the present invention, the multiple flow channels include a second flow channel; the second flow channel is provided with a flow channel buffer zone, and the shape of the flow channel buffer zone is first gradually increased and then gradually contracted along the extension direction of the second flow channel.

[0026] In an optional solution of the present invention, the main body is further provided with a sealed chamber, which is located below the flow channel buffer zone; the microfluidic device also includes a membrane and a conductive sheet, the membrane is arranged between the sealed chamber and the flow channel buffer zone, the membrane is arranged to allow ion transfer between the fluid in the sealed chamber and the fluid in the flow channel buffer zone, and the conductive sheet is located below the membrane and is used to transmit signals to the outside.

[0027] In an optional embodiment of the present invention, the body further comprises a tenth flow channel opening and an eleventh flow channel opening, both of which communicate with the sealed chamber and are used to inject the test liquid into the sealed chamber. In an optional embodiment of the present invention, a liquid reservoir is provided on the lower surface of the flow channel plate; the flow channel comprises a second section of a second flow channel, which comprises a flow buffer zone connected to the liquid reservoir. The liquid reservoir is sealed by a membrane and a lower plate, thereby forming a sealed chamber; the membrane is disposed between the liquid reservoir and the flow buffer zone, and the lower plate is provided with a conductive sheet at a position vertically overlapping the liquid reservoir.

[0028] In a second aspect, the present invention provides a microfluidic detection device, which includes a sensing device and the above-mentioned microfluidic device; the sensing device is arranged at the second flow channel and connected to the main body, and the sensing device and the main body form a corresponding part of the second flow channel together to form a sensing area.

[0029] Compared with the prior art, the present invention has the following beneficial effects:

[0030] The microfluidic device provided by the present invention includes a main body and a flow channel switching assembly, which is equipped with multiple flow channels. In this disclosure, the flow channel switching assembly alone can achieve multi-channel switching and on / off functions, resulting in a simple structure and easy operation. Furthermore, the flow channel switching assembly allows users to adjust the position of the flow channel according to different situations, connecting different flow channels in the main body to adapt to different usage scenarios, thus providing a more comprehensive function. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0032] FIG1a is a simplified diagram of the flow channel layout in a body according to one embodiment of the present disclosure;

[0033] FIG1 b is a schematic diagram of a flow channel switching assembly in a first position according to one embodiment of the present disclosure;

[0034] FIG1c is a schematic diagram of a flow channel switching assembly in a second position according to one embodiment of the present disclosure;

[0035] FIG1d is a schematic diagram of a flow channel switching assembly in a third position according to one embodiment of the present disclosure;

[0036] FIG2 a is a schematic diagram of a microfluidic device according to one embodiment of the present disclosure;

[0037] FIG2 b is an exploded view of the microfluidic device in FIG2 a ;

[0038] Figure 2c is a partial enlarged view of point E in Figure 2b;

[0039] FIG2 d is an exploded view of the flow channel switching assembly in FIG2 a ;

[0040] FIG3 a is an exploded view of a microfluidic device according to one embodiment of the present invention;

[0041] FIG3b is an exploded view of the body in FIG3a from another perspective;

[0042] FIG3c is an exploded view of the flow channel plate and the lower plate in FIG3a from another perspective;

[0043] FIG3 d is a cross-sectional view of the flow channel plate and the lower plate in FIG3 a from another perspective;

[0044] FIG3e is a top view of the flow channel plate in FIG3a;

[0045] FIG3 f is a bottom view of the flow channel plate in FIG3 a ;

[0046] FIG4 a is a schematic diagram of a microfluidic detection device according to one embodiment of the present disclosure;

[0047] FIG4 b is an exploded view of the microfluidic detection device of FIG4 a ;

[0048] FIG4 c is an exploded view of a microfluidic detection device according to one embodiment of the present disclosure;

[0049] FIG4 d is a partial cross-sectional view of the microfluidic detection device in FIG4 c ;

[0050] FIG4e is a simplified cross-sectional view of a microfluidic detection device according to one embodiment of the present disclosure;

[0051] FIG4f is a partial enlarged view of point F in FIG4e.

[0052] In the above drawings, the components represented by the reference numerals are as follows:

[0053] 1000. Microfluidic detection device;

[0054] 100, microfluidic device; 110, body; 111, upper plate; 112, flow channel plate; 1121, first protrusion; 1122, second protrusion; 113, lower plate; 1131, first guide post; 1132, second guide post; 120, flow channel switching assembly; 121, rotating body; 122, rotating shaft; 123, sealing plate; 124, limiting component; 130, fluid storage component; 140, membrane; 150, conductive sheet; 160, sealing patch;

[0055] 200, sensing device; 210, carrier board; 220, sensing chip; 230, probe;

[0056] 300, sealing gasket;

[0057] P1, first flow channel; P2, second flow channel; P21, first section of second flow channel; P22, second section of second flow channel; P23, third section of second flow channel; P3, third flow channel; P4, fourth flow channel;

[0058] C1, first flow channel outlet; C2, second flow channel outlet; C3, third flow channel outlet; C4, fourth flow channel outlet; C5, fifth flow channel outlet; C6, sixth flow channel outlet; C7, seventh flow channel outlet; C8, eighth flow channel outlet; C9, ninth flow channel outlet; C10, tenth flow channel outlet; C11, eleventh flow channel outlet;

[0059] B1, first liquid trough; B2, second liquid trough; B3, third liquid trough;

[0060] V, chute; V1, first chute section; V2, second chute section; V3, third chute section;

[0061] G1, first runner; G2, second runner; G21, first section of second runner; G22, second section of second runner; G3, third runner; G31, first section of third runner; G32, second section of third runner; G33, third section of third runner; G4, fourth runner;

[0062] H1, first through hole; H2, second through hole; H3, third through hole; H4, fourth through hole; H5, fifth through hole; H6, sixth through hole;

[0063] T1, first buffer tank; T2, second buffer tank;

[0064] D1, the first liquid outlet; D2, the second liquid outlet;

[0065] L, liquid storage tank; R, sealing chamber; Y, ring groove;

[0066] A1, sensing area; A2, flow channel buffer area; A3, observation area. DETAILED DESCRIPTION

[0067] In order to make the above and other features and advantages of the present invention more clear, the present invention is further described below with reference to the accompanying drawings. It should be understood that the specific embodiments given herein are for the purpose of explaining to those skilled in the art and are only exemplary and not restrictive.

[0068] The present disclosure provides a microfluidic device 100, which includes a main body 110 and a flow channel switching assembly 120. Figure 1a is a schematic diagram of the flow channel layout within the main body 110 provided according to one embodiment of the present disclosure. Referring to Figure 1a, the main body 110 is provided with a plurality of flow channels. Figures 1b to 1d are schematic diagrams of the connection relationship between the flow channels of the flow channel switching assembly 120 at different rotational positions. Referring to Figures 1b to 1d, the flow channel switching assembly 120 is rotatably connected to the main body 110. For example, the flow channel switching assembly 120 is provided with a shaft to achieve rotational connection with the main body 110, but is not limited to this, or through gears.

[0069] Furthermore, the channel switching assembly 120 is provided with a liquid flow groove, which is used to connect different channels. During the rotation of the channel switching assembly 120 relative to the main body 110, the position of the liquid flow groove also changes accordingly. When the liquid flow groove is located at a specific position, different channels can be connected.

[0070] In the present disclosure, the plurality of flow channels at least include a first flow channel P1, a second flow channel P2, and a third flow channel P3. The liquid transfer tank includes a first liquid transfer tank B1, a second liquid transfer tank B2, and a third liquid transfer tank B3.

[0071] Referring to Figure 1b , when the flow channel switching assembly 120 is in the first position, the first liquid transfer channel B1 connects the first flow channel P1 with the second flow channel P2. Referring to Figure 1c , when the flow channel switching assembly 120 is in the second position, the second liquid transfer channel B2 connects the second flow channel P2 with the third flow channel P3, and the third liquid transfer channel B3 connects the first flow channel P1 with the second flow channel P2.

[0072] Furthermore, referring to FIG. 1 d , the plurality of flow channels further include a fourth flow channel P4 . When the flow channel switching assembly 120 is located at the third position, the second liquid transfer tank B2 connects the third flow channel P3 with the fourth flow channel P4 .

[0073] As can be seen, in the present disclosure, there are four flow channels and three liquid flow channels. By switching the flow channel switching assembly 120 between the first position, the second position, and the third position, the liquid flow channel connects at least two of the multiple flow channels. Of course, the number of flow channels and the number of liquid flow channels are not limited to this. The number of flow channels and the number of liquid flow channels can be adjusted according to different application scenarios. Accordingly, the number of specific positions of the flow channel switching assembly 120 can also be adaptively adjusted, and no specific limitation is imposed herein.

[0074] Please refer to Figures 1a to 1d. In the present disclosure, each flow channel includes multiple flow channel openings. The first flow channel P1 includes a first flow channel opening C1 and a second flow channel opening C2. The second flow channel P2 includes a third flow channel opening C3 and a fourth flow channel opening C4. The third flow channel P3 includes a fifth flow channel opening C5 and a sixth flow channel opening C6. The fourth flow channel P4 includes an eighth flow channel opening C8 and a ninth flow channel opening C9.

[0075] Furthermore, the second to sixth flow channel openings C2 to C6 and the eighth flow channel opening C8 are arranged to overlap with the flow channel switching assembly 120 in the vertical direction.

[0076] It can be seen that in the present disclosure, at least one flow channel opening in each flow channel is overlapped with the flow channel switching assembly 120 in the vertical direction, so that the liquid flow tank can connect different flow channels through the flow channel opening.

[0077] It should be noted that the second flow channel P2 is used to form the sensing area A1, the third flow channel P3 is used to collect fluid, and the first flow channel P1 and the fourth flow channel P4 are used to drain fluid. It should be noted that the cross-sectional shape of the flow channel can be continuous and consistent along the extension direction, or it can be a variable flow channel cross-sectional shape, which is not specifically limited here.

[0078] In the present disclosure, the third flow channel opening C3 and the fourth flow channel opening C4 are both arranged to overlap with the flow channel switching component 120 in the upper and lower directions. In other words, when the flow channel switching component 120 is not in a specific position, the second flow channel P2 is a closed flow channel. It can be understood that since the second flow channel P2 is a closed flow channel, air is prevented from entering the sensing area A1.

[0079] It should be noted that the third flow channel P3, serving as a fluid collection area, can have various structural forms. For example, the third flow channel P3 may include a reservoir for storing fluid, or may be provided with a liquid-absorbing medium, such as absorbent cotton. In a preferred embodiment, the third flow channel P3 is a meandering and extending flow channel. In the illustrated embodiment, the third flow channel P3 has a serpentine structure, but this is not limiting and may also have a spiral structure, for example.

[0080] In an optional embodiment, the third flow channel P3 also includes a seventh flow channel opening C7, which is located on one side of the flow channel switching component 120, that is, the seventh flow channel opening C7 and the flow channel switching component 120 do not overlap in the upper and lower directions, so that the fluid collected in the third flow channel P3 can be discharged through the seventh flow channel opening C7.

[0081] Referring to Figure 1b , when the flow channel switching assembly 120 is in the first position, the first liquid channel B1 connects the second liquid channel C2 and the third liquid channel C3, thereby connecting the first flow channel P1 and the second flow channel P2. The third flow channel P3 and the fourth flow channel P4 are independent. Understandably, in this position, fluid can be injected from the first flow channel opening C1, passing through the first flow channel P1 and into the second flow channel P2, thereby supplying the fluid to the sensing area A1.

[0082] Referring to Figure 1c, when the flow channel switching assembly 120 is in the second position, the third liquid channel B3 connects the second liquid channel C2 and the third liquid channel C3, and the second liquid channel B2 connects the fourth liquid channel C4 and the fifth liquid channel C5, thereby sequentially connecting the first flow channel P1, the second flow channel P2, and the third flow channel P3. It can be understood that in this position, fluid can be injected into the first flow channel port C1, flow through the first flow channel P1 into the second flow channel P2, and then flow from the second flow channel P2 into the third flow channel P3. Fluid passing through the sensing area A1 can be temporarily stored in the third flow channel P3.

[0083] Referring to Figure 1d, when the flow channel switching assembly 120 is in the third position, the second liquid channel B2 connects the sixth liquid channel C6 and the eighth liquid channel C8, thereby connecting the third flow channel P3 and the fourth flow channel P4. The first flow channel P1 and the second flow channel P2 are independent. It can be understood that in this position, the fluid collected in the third flow channel P3 can be discharged through the seventh flow channel C7 or the ninth flow channel C9.

[0084] As can be seen, in the present disclosure, the first flow channel opening C1, the seventh flow channel opening C7, and the ninth flow channel opening C9 serve as injection ports or liquid extraction ports, injecting fluid into the flow channel through the injection operation and draining the fluid from the flow channel through the liquid extraction operation. The second flow channel opening C2 to the sixth flow channel opening C6 and the eighth flow channel opening C8 serve as intermediate liquid flow channel openings. It should be noted that the shape of the flow channel opening can be a circular opening, a square opening, or a bell-shaped opening, etc. The specific shape of the flow channel opening is not limited and can be adjusted according to actual needs.

[0085] It is understood that when the flow channel switching assembly 120 is in the second position, the fluid collected in the third flow channel P3 can also be extracted through the seventh flow channel opening C7. This operation will draw air from the first flow channel P1 into the second flow channel P2, thereby mixing air in the sensing area A1 and affecting the experimental results. Therefore, after the flow channel switching assembly 120 is switched to the third position, the second flow channel P2 is isolated, and the fluid collected in the third flow channel P3 is then discharged through the seventh flow channel opening C7 or the ninth flow channel opening C9. This can prevent air from being drawn into the second flow channel P2 and thus prevent air from mixing in the sensing area A1.

[0086] In summary, in the present disclosure, multiple flow channel switching and on / off functions can be achieved solely through the flow channel switching assembly 120, resulting in a simple structure, easy operation, and low cost. Furthermore, users can adjust the position of the liquid tank using the flow channel switching assembly 120 to connect different flow channels in the body 110 to suit different usage scenarios, providing a more comprehensive functionality.

[0087] Figure 2a is a schematic diagram of a microfluidic device 100 according to one embodiment of the present disclosure. Figure 2d is an exploded view of the flow channel switching assembly 120 in Figure 2a. Referring to Figure 2d, the flow channel switching assembly 120 includes a rotating body 121, a rotating shaft 122, a sealing plate 123, and a limiting component 124. The rotating shaft 122 and the limiting component 124 are both connected to the rotating body 121. The rotating body 121 is rotatably connected to the main body 110 via the rotating shaft 122. Accordingly, the limiting component 124 can rotate with the rotating body 121.

[0088] In the present disclosure, the sealing plate 123 is connected to the rotating body 121 and can thus rotate with the rotating body 121. In a specific application, the sealing plate 123 passes through the rotating shaft 122 and is attached to the rotating body 121. The sealing plate 123 can be attached to the rotating body 121 by heat fusion, ultrasonic welding, laser welding, gluing, or other methods. It should be noted that the upper surface of the sealing plate 123 is attached to the rotating body 121, and the lower surface is attached to the main body 110. The lower surface seals the flow channel opening, indicating that the sealing plate 123 is located between the rotating body 121 and the main body 110.

[0089] In the present disclosure, the first through third liquid transfer channels B1, B3 are all located on the sealing plate 123. As the sealing plate 123 rotates with the rotating body 121, the positions of the various transfer channels can be adjusted to connect different flow paths. In specific applications, the transfer channels are all strip-shaped, with their dimensions and placement tailored to the flow path openings to facilitate connection between the various flow paths. Of course, the present disclosure does not impose any specific restrictions on the shape of the transfer channels; they only need to ensure that they can connect the flow path openings of different flow paths.

[0090] Figure 2b is an exploded view of the microfluidic device 100 in Figure 2a. Figure 2c is a partial enlarged view of point E in Figure 2b. Furthermore, in the present disclosure, the body 1 is provided with a chute V. After the rotating body 121 is connected to the body 1, at least a portion of the limiting component 124 is embedded in the chute V. During the rotation of the flow channel switching assembly 120 relative to the body 110, the limiting component 124 slides along the chute V.

[0091] In a specific application, the chute V is an arc-shaped strip groove to adapt to the movement trajectory of the limiting component 124. It can be understood that the cooperation between the limiting component 124 and the chute V limits the relative rotation angle of the flow channel switching assembly 120 and the body 110, so that the flow channel switching assembly 120 can only switch between the first position, the second position, and the third position.

[0092] In the present disclosure, the slide groove V includes a first slide groove section V1, a second slide groove section V2 and a third slide groove section V3, and the limiting component 124 is configured to slide along the first slide groove section V1 to the first position, slide along the second slide groove section V2 to the third position, and slide along the third slide groove section V3 to rotate to the second position.

[0093] In an optional embodiment, the limiting component 124 is a limiting pin, the bottom end of which extends into the chute V. A shallow pit is provided on the bottom wall of each of the first chute segment V1, the second chute segment V2, and the third chute segment V3. During rotation of the flow channel switching assembly 120, the bottom end of the limiting pin can be embedded in the shallow pit in any chute segment, thereby creating a sense of hysteresis during rotation to confirm that the rotation is in place. Of course, the depth of the shallow pit should be appropriate to ensure that the limiting pin can be removed from the shallow pit and move to the next chute segment when the rotational torque is increased.

[0094] Referring to Figure 2c, in the present disclosure, the depth of the second chute segment V2 is equal to the depth of the third chute segment V3. The depths of both the second and third chute segments V2 and V3 are greater than the depth of the ground chute segment V1. In other words, the chute V is formed by joining two chute segments of different depths. The two ends of the chute V correspond to the first and second positions, respectively, and the junction of the two chute segments corresponds to the third position. Thus, the structural features of the chute V itself define the rotational position of the flow channel switching assembly 120.

[0095] In an optional embodiment, the limiting component 124 is a preloaded spring pin, the extension length of which can be adaptively extended and retracted as the groove depth changes. To facilitate understanding and explanation of this embodiment, the chute section formed by splicing the second chute section V2 and the third chute section V3 is referred to as a combined chute section, and the groove depth of the combined chute section is greater than the groove depth of the first chute section V1. When the preloaded spring pin is located at the end of the first chute section V1, the flow channel switching assembly 120 is in the first position; when the preloaded spring pin moves out of the first chute section V1 and is located at the junction of the first chute section V1 and the combined chute section, the preloaded spring pin is ejected, and the flow channel switching assembly 120 is in the third position; when the preloaded spring pin moves to the end of the combined chute section, the flow channel switching assembly 120 is in the second position.

[0096] In a preferred embodiment, the difference in depth between the first chute section V1 and the combined chute section is sufficiently large that, when the preload spring pin is removed from the first chute section V1, the preload spring pin extends sufficiently far to prevent it from sliding back from the combined chute section to the first chute section V1. Thus, in this embodiment, the stop member 124 cannot slide back to the first chute section V1 when it is located in the second chute section V2 or the third chute section V3.

[0097] It should be noted that the structure that prevents the limiting component 124 from sliding back to the first chute section V1 is not limited to this. For example, the limiting component 124 is a pre-loaded spring pin, the groove depths of the first chute section V1, the second chute section V2 and the third chute section V3 are the same, and the bottom wall of the junction of the first chute section V1 and the other chute sections is provided with a wedge-shaped boss extending upward, and the wedge-shaped boss is configured to allow the pre-loaded spring pin to slide out of the first chute section V1 and prohibit it from sliding into other chute sections.

[0098] Referring to Figures 2b and 2c , in the illustrated embodiment, the limiting component 124 is a preloaded spring pin, the chute V is a semicircular arc, with the first chute segment V1 and the merging chute segment each occupying half, and the flow channel switching assembly 120 is allowed to rotate between 0° and 180°. It should be noted that when the preloaded spring pin is located at the end of the first chute segment V1, the rotation angle of the flow channel switching assembly 120 is 0°, corresponding to the first position. Rotating the flow channel switching assembly 120 counterclockwise by 90°, the preloaded spring pin pops out and is located at the junction of the first chute segment V1 and the merging chute segment, corresponding to the third position. Rotating the flow channel switching assembly 120 counterclockwise by another 90°, the rotation angle reaches 180°, and the preloaded spring pin is located at the end of the merging chute segment, corresponding to the second position. Figure 2a shows the flow channel switching assembly 120 in the second position. It should be noted that, since the first chute section V1 and the combined chute section are deeper, a step is formed to hinder the movement of the preload spring pin. After the preload spring pin slides into the combined chute section, it cannot slide back to the first chute section V1.

[0099] Of course, the rotation angle range and the limit positions of the flow channel switching component 120 are not limited to the illustrated embodiment. The rotation angle range and the limit positions can be adjusted accordingly by adjusting the arc length of the chute V and the proportion of each chute segment.

[0100] In summary, in the present disclosure, the flow channel switching component 120 is essentially a three-position valve, which cooperates with the slide groove V to enable the flow channel switching component 120 to switch between the first position, the second position and the third position, thereby achieving the purpose of controlling the on and off of the four flow channels.

[0101] Figure 3a is an exploded view of a microfluidic device 100 according to one embodiment of the present invention. Referring to Figure 3a , in the illustrated embodiment, the body 110 is a multi-plate assembly structure comprising an upper plate 111, a flow channel plate 112, and a lower plate 113. Specifically, the upper plate 111, the flow channel plate 112, and the lower plate 113 are assembled from top to bottom to form the body 110. In specific applications, the plates can be assembled using processes such as bonding, ultrasonic welding, laser welding, or gluing.

[0102] Figure 3e is a top view of the flow channel plate 112 in Figure 3a; Figure 3f is a bottom view of the flow channel plate 112 in Figure 3a. Referring to Figures 3e and 3f, flow channel grooves are provided on both the upper and lower surfaces of the flow channel plate 112. The upper plate 111 and the lower plate 113 are connected to the upper and lower surfaces of the flow channel plate 112, respectively, thereby covering the flow channel grooves and forming multiple flow channels. As can be understood, the multiple flow channels are arranged on the upper and lower sides of the flow channel plate 112, forming a double-layer flow channel structure. This significantly reduces the space occupied by the microfluidic device 100, achieving the goal of miniaturization.

[0103] Figure 3b is an exploded view of the main body 110 in Figure 3a from another perspective. Please refer to Figure 3b. In the present disclosure, the flow channel plate 112 is provided with a first flow channel groove G1, a second flow channel groove G2, a third flow channel groove G3 and a fourth flow channel groove G4. The first flow channel groove G1 is used to form a first flow channel P1, the second flow channel groove G2 is used to form a second flow channel P2, the third flow channel groove G3 is used to form a third flow channel P3, and the fourth flow channel groove G4 is used to form a fourth flow channel P4.

[0104] Referring to Figure 3e, the upper surface of the flow channel plate 112 is provided with a first flow channel groove G1 and a fourth flow channel groove G4. Referring to Figure 3b, the upper plate 111 is provided with first to ninth flow channel openings C1 to C9. The first and second flow channel openings C1 and C2 communicate with the first flow channel groove G1, forming the first flow channel P1 when the upper plate 111 is attached to the flow channel plate 112. The eighth and ninth flow channel openings C8 and C9 communicate with the fourth flow channel groove G4, forming the fourth flow channel P4 when the upper plate 111 is attached to the flow channel plate 112. Thus, the first and fourth flow channel grooves G1 and G4, together with the upper plate 111, form the first and fourth flow channels P1 and P4.

[0105] It can be seen that in the present disclosure, the first flow channel groove G1 and the fourth flow channel groove G4 are both single flow channel groove segments. Of course, the first flow channel groove G1 and the fourth flow channel groove G4 can also be composed of multiple flow channel groove segments. Secondly, the first flow channel groove G1 and the fourth flow channel groove G4 can also be arranged on the lower surface of the flow channel plate 112, and no specific restrictions are made in the present disclosure.

[0106] Referring to Figures 3e and 3f , in the present disclosure, the third flow channel G3 includes a first section G31, a second section G32, and a third section G33. The first and third sections G31 and G33 are located on the upper surface of the flow channel plate 112, while the second section G32 is located on the lower surface of the flow channel plate 112. As shown in Figure 3b , the fifth and sixth flow channel openings C5 and C6 connect to the first section G31, and the seventh flow channel opening C7 connects to the third section G33.

[0107] Furthermore, the flow channel plate 112 is provided with a third through hole H3 and a fourth through hole H4, and the third through hole H3 and the fourth through hole H4 are respectively located at the two ends of the second section G32 of the third flow channel groove, and are respectively connected to the first section G31 of the third flow channel groove and the third section G33 of the third flow channel groove. In this way, the first section G31 of the third flow channel groove, the second section G32 of the third flow channel groove and the third section G33 of the third flow channel groove are connected in sequence to form the third flow channel groove G3, and the third flow channel P3 is formed when the upper plate 111 and the lower plate 113 respectively cover the upper surface and the lower surface of the flow channel plate 112.

[0108] It can be seen that in the present disclosure, the third flow channel G3 is formed by three flow channel segments connected in series. It can be understood that the structural form and layout position of the third flow channel G3 can be adjusted by increasing the number of flow channel segments and through holes, and no specific limitation is made here.

[0109] As previously mentioned, in an optional embodiment, the third flow channel P3 is a circuitous and extending flow channel to facilitate fluid collection. Understandably, the third flow channel P3 occupies a relatively large space. Therefore, the third flow channel P3 is arranged on the upper and lower sides of the flow channel plate 112, resulting in a stacked structure that facilitates miniaturization.

[0110] Please refer to FIG. 3 b , the lower plate 113 is provided with a first buffer tank T1 and a second buffer tank T2 . The first buffer tank T1 is provided with a first liquid port D1 , and the second buffer tank T2 is provided with a second liquid port D2 .

[0111] Referring to Figures 3e and 3f, the second flow channel G2 includes a second flow channel first section G21, which is disposed on the upper surface of the flow channel plate 112. The flow channel plate 112 is provided with a first through hole H1, which is located at the end of the second flow channel first section G21. As shown in Figure 3b, the third flow channel opening C3 is connected to the second flow channel first section G21. Figure 3c is an exploded view of the flow channel plate 112 and the lower plate 113 in Figure 3a from another perspective. As shown in Figure 3c, the first through hole H1 connects the second flow channel first section G21 to the first buffer tank T1. When the upper plate 111 and the lower plate 113 are respectively covered with the upper and lower surfaces of the flow channel plate 112, the second flow channel first section P21 is formed.

[0112] Referring to Figures 3e and 3f , the second flow channel G2 also includes a second flow channel second section G22, which is disposed on the upper surface of the flow channel plate 112. The flow channel plate 112 also includes a second through hole H2, located at the end of the second flow channel second section G22. As shown in Figure 3b , the fourth flow channel opening C4 connects to the second flow channel second section G22. As shown in Figure 3c , the second through hole H2 connects the second flow channel second section G22 to the second buffer tank T2. When the upper plate 111 and the lower plate 113 are respectively attached to the upper and lower surfaces of the flow channel plate 112, the second flow channel second section P22 is formed.

[0113] It can be understood that the first section P21 of the second flow channel and the second section P22 of the second flow channel can be communicated with each other through the first liquid transfer port D1 and the second liquid transfer port D2.

[0114] Furthermore, in the present disclosure, the shape of the first buffer groove T1 is configured to gradually expand from the side away from the first liquid outlet D1 to the side closer to the first liquid outlet D1; the shape of the second buffer groove T2 is configured to gradually expand from the side away from the second liquid outlet D2 to the side closer to the second liquid outlet D2. In other words, the groove width on the side closer to the liquid outlet is greater than the groove width on the side away from the liquid outlet. This results in a gradual decrease in the flow rate of the fluid flowing out of the body 110 through the first liquid outlet D1 and a gradual increase in the flow rate of the fluid entering the body 110 through the second liquid outlet D2. Referring to Figure 3b, in the illustrated embodiment, the first buffer groove T1 and the second buffer groove T2 are generally isosceles trapezoidal in shape, but this is not limited to this and may also be semicircular in shape, for example.

[0115] Furthermore, in the present disclosure, the bottom surface of the first buffer tank T1 is tilted downward from the side away from the first liquid transfer port D1 toward the side closer to the first liquid transfer port D1; the bottom surface of the second buffer tank T2 is tilted downward from the side away from the second liquid transfer port D2 toward the side closer to the second liquid transfer port D2. In other words, the bottom surface of the buffer tank is inclined, which avoids the formation of a right angle at the junction of the through hole and the buffer tank, thereby preventing fluid accumulation at the right angle, ensuring smooth fluid flow. Of course, the inclined structure also avoids excessive height difference and reduces the risk of bubble generation.

[0116] Figure 3d is a cross-sectional view of the flow channel plate 112 and lower plate 113 in Figure 3a from another perspective. Referring to Figures 3c and 3d, in a specific application, the flow channel plate 112 is provided with downwardly extending protrusions, comprising a first protrusion 1121 and a second protrusion 1122. Both the first protrusion 1121 and the second protrusion 1122 have inclined surfaces and are respectively embedded in the first buffer groove T1 and the second buffer groove T2, thereby forming inclined flow channel sections. In other words, the first section P21 of the second flow channel and the second section P22 of the second flow channel both include inclined flow channel sections. This prevents fluid accumulation caused by excessive height differences during the process of fluid sinking or rising, ensuring smooth fluid flow.

[0117] Referring to Figure 2a , in an optional embodiment, the body 110 is provided with a transparent observation area A3 located above the second flow channel P2. In specific applications, the vertical projection of the sensing area A3 covers the first liquid outlet D1 and the second liquid outlet D2, facilitating observation of the sensing area A1. Referring to Figures 2a and 3a , in actual applications, the observation area A3 has openings in the projection areas of both the upper plate 111 and the flow channel plate 112 , and the projection area on the lower plate 113 is configured as a transparent area, or the entire lower plate 113 is made of a transparent material (e.g., a transparent acrylic plate). This forms the observation area A3 for convenient viewing of the sensing area A1.

[0118] Referring to Figures 2a and 2b , in an optional embodiment, the microfluidic device 100 further includes a fluid storage component 130, which is connected to the body 110 and communicates with the first flow channel C1. In this embodiment, the first flow channel C1 serves as an inlet. The fluid storage component 130 includes a liquid collection chamber, which is open at the top and connected to the upper plate 111 at the bottom and communicates with the first flow channel C1. This chamber is used to temporarily store the fluid to be injected. In this embodiment, the liquid collection chamber of the fluid storage component 130 is wide at the top and narrow at the bottom to facilitate fluid flow toward the first flow channel C1. Preferably, the fluid storage component 130 is detachably connected to the body 110. In specific applications, the bottom end of the fluid storage component 130 abuts the upper plate 111 and has an opening for docking with the first flow channel C1. The upper plate 111 supports and stabilizes the fluid storage component 130, ensuring that no leakage occurs during the injection process. This achieves a detachable connection and facilitates replacement.

[0119] Referring to Figure 1a, the second flow channel P2 is provided with a flow buffer zone A2. The shape of the flow buffer zone A2 is arranged to gradually increase and then gradually decrease along the extension direction of the second flow channel P2. In the illustrated embodiment, the flow buffer zone A2 is located between the sensing area A1 and the fourth flow channel opening C4. With the first flow channel opening C1 serving as the liquid injection port, the flow buffer zone A2 is located downstream of the sensing area A1. Of course, the flow buffer zone A2 can also be located between the sensing area A1 and the third flow channel opening C3, in which case the flow buffer zone A2 is located upstream of the sensing area A1. In other words, the flow buffer zone A2 can be located either upstream or downstream of the sensing area A1, without specific limitation.

[0120] Furthermore, in the present disclosure, the body 110 is further provided with a sealed chamber R, which is located below the second flow channel P2. Preferably, the sealed chamber R is located below the flow channel buffer zone A2. In a specific application, the microfluidic device 100 further includes a membrane 140 and a conductive sheet 150. The membrane 140 is provided between the sealed chamber R and the flow channel buffer zone A2, and the sealed chamber R and the flow channel buffer zone A2 are separated by the membrane 140. The membrane 140 is configured to allow ion transfer between the fluid in the sealed chamber R and the fluid in the flow channel buffer zone A2, so that only ions can be exchanged between the fluid in the flow channel buffer zone A2 and the fluid in the sealed chamber R. The conductive sheet 150 is located below the membrane 140 and is used to transmit signals generated by ion exchange.

[0121] It should be noted that the membrane 140 is a highly permeable membrane or a semi-permeable membrane, such as an ion exchange membrane, a proton exchange membrane, etc., which can be manufactured according to actual functional requirements and is not specifically limited here.

[0122] It can be understood that the larger the groove width at the flow channel buffer area A2 , the lower the flow rate of the fluid passing through the flow channel buffer area A2 , which is beneficial for ion exchange between the fluid in the flow channel and the fluid in the sealed chamber R through the membrane 140 .

[0123] Referring to Figures 3a and 3c , in the present disclosure, a liquid reservoir L is provided on the lower surface of the flow channel plate 112. The flow channel buffer zone A2 is located in the second section G22 of the second flow channel and communicates with the liquid reservoir L. The liquid reservoir L is sealed by the membrane 140 and the lower plate 113, thereby forming a sealed chamber R. In a specific application, a through opening is formed in the bottom wall of the flow channel buffer zone A2 at a position corresponding to the second section G22 of the second flow channel, communicating with the liquid reservoir L. The membrane 140 is sealingly attached to the through opening. Therefore, in the present disclosure, the membrane 140 is located between the liquid outlet trough L and the flow channel buffer zone A2, thereby separating the sealed chamber R from the second section G22 of the second flow channel.

[0124] Furthermore, the lower plate 113 is provided with a conductive sheet 150 at a position overlapping with the liquid storage tank L in the up and down directions. The conductive sheet 150 and the membrane 140 can be infiltrated by the fluid located in the sealed chamber R, and the signal generated during the ion exchange between the fluid in the sealed chamber R and the fluid in the flow channel buffer zone A2 can be transmitted outward through the conductive sheet 150.

[0125] Referring to FIG. 3 a , in a specific application, the lower plate 113 is provided with an opening for mounting a conductive sheet 150 . The opening vertically overlaps the liquid reservoir L, i.e., the opening communicates with the liquid reservoir L and is sealed by the conductive sheet 150 . The conductive sheet 150 can be made of a conductive metal sheet, and its shape is not specifically limited herein.

[0126] Referring to Figures 3b and 3c, the upper plate 111 further defines a tenth flow channel opening C10 and an eleventh flow channel opening C11. The flow channel plate 112 further defines a fifth through-hole H5 and a sixth through-hole H6. The tenth flow channel opening C10 communicates with the liquid flow channel L via the fifth through-hole H5, and the eleventh flow channel opening C11 communicates with the liquid flow channel L via the sixth through-hole H6. In other words, both the tenth flow channel opening C10 and the eleventh flow channel opening C11 communicate with the sealed chamber R. It can be understood that one of the tenth flow channel opening C10 and the eleventh flow channel opening C11 is used to inject the testing liquid into the sealed chamber R, while the other is used to expel air from the sealed chamber R. This ensures that the testing liquid is smoothly injected into the sealed chamber. Therefore, the tenth flow channel opening C10 and the eleventh flow channel opening C11 are used to inject the testing liquid.

[0127] 2b , in an optional embodiment, the microfluidic device 100 further includes a sealing sticker 160 . After the liquid is injected into the sealed chamber R, the sealing sticker 160 seals the tenth flow channel opening C10 and the eleventh flow channel opening C11 to prevent leakage of the detection liquid in the sealed chamber R.

[0128] It should be noted that, in the above embodiment, the sealed chamber R is formed by the liquid storage tank L, the membrane 140 and the lower plate 113, but it is certainly not limited to this. For example, the sealed chamber R is formed in a sealed container provided with the membrane 140 and the conductive sheet 150, and the sealed container is embedded in the main body 110, and can even be detachably connected to the main body 110. It can be seen that the structural form and shape of the sealed chamber R can be diverse, and no specific restrictions are made here.

[0129] It should be noted that in the present disclosure, the main body 110 is a structure in which three plate-like components are stacked and assembled in the vertical direction, but it is not limited to this. For example, in an optional embodiment, the main body 110 provided with various flow channels and a liquid storage tank L is made by an integrated molding process, for example, by 3D printing; then the membrane 140 and the conductive sheet 150 are assembled to seal the liquid storage tank L to form a sealed chamber R, and then the flow channel switching assembly 120, the fluid storage component 130 and the sealing sticker 160 are assembled. It can be understood that the production cost is relatively high under this method, especially the assembly of the membrane 140 is difficult. Alternatively, in another optional embodiment, the flow channel plate 112 and the upper plate 111 or the lower plate 113 are made by an integrated molding process, and the main body 110 is made by a two-plate assembly structure, thus avoiding the difficulty of assembling the membrane 140, but its production cost is relatively high. It can be seen that the structure of the main body 110 is not limited to this embodiment, and no specific restrictions are made here.

[0130] FIG4 a is a schematic diagram of a microfluidic detection device 1000 according to one embodiment of the present disclosure. Referring to FIG4 a , the microfluidic detection device 1000 includes the aforementioned microfluidic device 100 and a sensing device 200 .

[0131] Figure 4b is an exploded view of the microfluidic detection device 1000 of Figure 4a. Referring to Figure 4b, the sensing device 200 is connected to the main body 110. The vertical projection of the sensing device 200 covers the first liquid port D1 and the second liquid port D2. Figure 4d is a partial cross-sectional view of the microfluidic detection device of Figure 4c. For ease of viewing, the upper plate 111 is hidden in Figure 4d. The sensing device 200 is connected to the main body 110 to form a third section P23 of the second flow channel. The third section P23 of the second flow channel is connected to the first section P21 and the second section P22 of the second flow channel through the first liquid port D1 and the second liquid port D2, respectively, thereby forming the second flow channel P2. In the present disclosure, the location corresponding to the third section P23 of the second flow channel is the sensing area A1. The vertical projection of the sensing area A1 covers the first liquid port D1 and the second liquid port D2.

[0132] Please refer to FIG4 b . In the present disclosure, the sensing device 200 and the microfluidic device 100 are connected by screws. Of course, this is not limited to this. For example, pins, bolts and nuts may also be used.

[0133] It should be noted that, in the above description, the first flow channel opening C1 serves as a liquid injection port and its projection in the up and down directions does not overlap with the flow channel switching component 120. Therefore, the first flow channel opening C1 is located on one side of the flow channel switching component 120. Correspondingly, the fluid storage component 130 is located on one side of the flow channel switching component 120, and the two are arranged at intervals.

[0134] It should be noted that when a liquid injection port is provided above the sensing area A1, the downward flow channel for introducing the fluid into the sensing area A1 is relatively short, and bubbles are easily introduced. It can be understood that the first flow channel opening C1 is not located above the sensing area A1, which reduces the risk of introducing bubbles. Secondly, the flow channel switching component 120 is not located above the sensing area A1, which is convenient for observation through the observation area A3. In the present disclosure, the circulation storage component 130 and the flow channel switching component 120 are located on the same side or adjacent sides of the sensing area A1 to ensure that the projections of the first flow channel opening C1 and the flow channel switching component 120 in the upper and lower directions do not overlap with the sensing area A1. Of course, such a layout is also conducive to miniaturization design.

[0135] Please refer to Figure 4b. In the present disclosure, the microfluidic detection device 1000 also includes a sealing gasket 300. The sealing gasket 300 is arranged between the sensing device 200 and the body 110. The sealing gasket 300 is arranged around the sensing area A1, that is, around the third section P23 of the second flow channel, thereby sealing the third section P23 of the second flow channel to prevent fluid from leaking from between the microfluidic device 100 and the sensing device 200.

[0136] It is understandable that since the sealing gasket 300 is disposed around the sensing area A1, in an optional embodiment, the sealing gasket 300 is an annular structure. Of course, it is not limited to this. For example, the sealing gasket 300 can be assembled from two semi-annular structures to ensure that there is no leakage.

[0137] Figure 4c is an exploded view of a microfluidic detection device 1000 according to one embodiment of the present disclosure. Referring to Figure 4c, in the present disclosure, the sensing device 200 includes a carrier 210, a sensing chip 220, and a probe 230. The sensing chip 220 is located in the sensing area A1. In a specific application, the sensing chip 220, the sealing gasket 300, and the microfluidic device 100 together form the second flow channel third section P23. The sensing chip 220 can be wetted by fluid passing through the second flow channel third section P23.

[0138] As mentioned above, the sealing gasket 300 is annular in structure, and thus a sealing gasket opening is formed. The upper surface of the sensing chip 220 can be embedded in the sealing gasket opening, thereby contacting the fluid in the third section P23 of the second flow channel.

[0139] Referring to Figure 4c, probe 230 is further connected to carrier 210, with its tip abutting conductive sheet 150. Figure 4e is a simplified cross-sectional view of a microfluidic detection device 1000 according to one embodiment of the present invention. Figure 4e is not a cross-sectional view of an actual product, but rather a schematic diagram to illustrate the relationship between sensing area A1, flow channel buffer area A2, and sealed chamber R. Figure 4f is a partial enlarged view of point F in Figure 4e.

[0140] Referring to Figures 4e and 4f , both sensing area A1 and flow channel buffer area A2 are located within the second flow channel P2. It is understood that fluid passing through the second flow channel P2 can infiltrate the sensing chip 220 located within the sensing area A1 and the membrane 140 located within the flow channel buffer area A2. As previously described, the signal generated by ion exchange between the detection fluid in the sealed chamber R and the fluid in the second flow channel P2 is transmitted to the carrier 210 via the conductive sheet 150 and the probe 230. Furthermore, the signal generated by the sensing chip 220 is transmitted to the carrier 210. Thus, the signal generated by the sensing device 200 is transmitted outwardly through the carrier 210, for example, to be collected by a signal acquisition and analysis instrument, thereby completing the detection and analysis.

[0141] It should be noted that the probe 230 can be in a variety of forms, as long as it can be electrically connected to the conductive sheet 150, and can be, for example, an electrode. The carrier 210 can be a PCB to ensure signal transmission between the sensing chip 220 and the probe 230. The sensing chip 220 can be a biochip, and the sensing chip 220 is integrated with the carrier 210 to form the main body of the sensing device 200.

[0142] As mentioned above, in a preferred embodiment, the flow channel plate 112 is provided with a first buffer groove T1 and a second buffer groove T2. The buffer groove structure prevents the fluid from flowing into the sensing area A1 from flowing too fast, ensures that the contact time between the fluid and the sensing chip 220 is appropriate, and avoids the generation of bubbles.

[0143] Furthermore, in the present disclosure, the body 110 is provided with an annular groove Y surrounding the sensing area A1, with at least a portion of the sealing gasket 300 embedded within the annular groove Y. Specifically, when installing the sensing device 200 into the microfluidic device 100, the sealing gasket 300 is first pre-fixed within the annular groove Y before the sensing device 200 is installed, thereby facilitating installation. It is understood that since the groove Y surrounds the sensing area A1, the sealing gasket 300 installed within the annular groove Y also surrounds the sensing area A1.

[0144] Furthermore, in the present disclosure, the body 110 is further provided with a plurality of guide posts extending downward. These guide posts are located within the area enclosed by the annular groove Y, and the sealing gasket 300 is connected to the guide posts. Referring to FIG. 4 b , in actual use, the guide posts are disposed on the lower plate 113 and include a first guide post 1131 and a second guide post 1132. The sealing gasket 300 is provided with two corresponding guide post mounting holes. The guide posts can pass through the guide post mounting holes, allowing the sealing gasket 300 to move along the guide posts into the annular groove Y, thereby enabling rapid positioning and installation of the sealing gasket 300. Of course, the number and arrangement of the guide posts are not limited to this.

[0145] In the present disclosure, the microfluidic detection device 1000 is configured to have a first mode and a second mode. The flow channel switching assembly 120 is rotated to connect different flow channels, thereby switching the microfluidic detection device 1000 between the first and second modes. It can be understood that rotating the flow channel switching assembly 120 adjusts the position of the liquid channel, thereby connecting different flow channels through the liquid channel.

[0146] Furthermore, in the first mode, the flow channel switching assembly 120 connects the first flow channel P1 with the second flow channel P2, and the third flow channel P3 and the fourth flow channel P4 are independent. In other words, in the first mode, the flow channel switching assembly 120 rotates to the first position.

[0147] In the second mode, the flow channel switching assembly 120 sequentially connects the first flow channel P1, the second flow channel P2, and the third flow channel P3, while the fourth flow channel P4 remains independent. Alternatively, the first flow channel P1 and the second flow channel P2 are independent, while the third flow channel P3 and the fourth flow channel P4 are connected. In other words, in the second mode, the flow channel switching assembly 120 rotates to the second position or the third position. It should be noted that, in the above description, an independent flow channel means that the flow channel is not connected to other flow channels.

[0148] In specific applications, the application scenarios of the microfluidic detection device 1000 are divided into user mode and administrator mode. In the present disclosure, the first mode is the administrator mode and the second mode is the user mode.

[0149] Therefore, in administrator mode, the flow channel switching assembly 120 is in the first position, connecting the first flow channel P1 and the second flow channel P2. At this time, fluid can be introduced into the second flow channel P2 through the first flow channel P1. Referring to Figures 1a and 1b, fluid is injected through the first liquid injection port C1 and passes through the first flow channel P1, the first liquid transfer tank B1, the first section P21 of the second flow channel, the sensing area A1, and the second section P22 of the second flow channel including the flow channel buffer area A2.

[0150] It should be noted that the surface of the sensing chip 220 has a microcavity structure. In a preferred production process, the microcavity structure in the sensing chip 220 needs to be filled with reagents through vacuum negative pressure means. Since a vacuum environment needs to be constructed, this process is carried out in the production plant. Before leaving the factory, the microfluidic detection device 1000 is set to administrator mode to pre-package the reagent in the second flow channel P2 and fill it in the microcavity structure of the sensing chip 220.

[0151] As can be seen from the above, the user mode includes two conditions. In the first condition, the flow channel switching assembly 120 is in the second position, and the first flow channel P1, the second flow channel P2, and the third flow channel P3 are connected in sequence. Referring to Figures 1a and 1c, fluid is injected through the first liquid injection port C1 and passes through the first flow channel P1, the third flow channel groove B3, the second flow channel first section P21, the sensing area A1, the second flow channel second section P22 including the flow channel buffer zone A2, the second liquid transfer groove B2, and the third flow channel P3. Finally, the fluid can be temporarily stored in the third flow channel P3.

[0152] It can be seen that in this case, the fluid passes through the sensing area A1 and the flow channel buffer A2. In this case, the user can inject the reagent required for sensing into the sensing area A1 and perform ion transfer with the detection liquid in the sealed chamber R below the flow channel buffer A2.

[0153] In the second scenario, the flow channel switching assembly 120 is in the third position, connecting the third flow channel P3 to the fourth flow channel P4. Referring to Figures 1a and 1d, with the ninth flow channel opening C9 serving as the liquid extraction port, the fluid in the third flow channel P3 flows through the second liquid transfer tank B2 into the fourth flow channel P4 and then out of the ninth flow channel opening C9. This completes the collection of waste liquid after sensing and prevents the introduction of bubbles into the sensing area A1.

[0154] It can be seen that in the present disclosure, different flow channels are connected by switching the flow channel switching component 120, so that the microfluidic detection device 1000 switches between three states to adapt to different modes, thereby taking into account the negative pressure pre-packaging process and the user's injection situation, meeting the common needs of production and user use, and having a wider application scenario.

[0155] As can be seen from the above, when the limiting component 124 is in the first chute section V1, it is in administrator mode, and when it is in the second chute section V2 or the third chute section V3, it is in user mode. In a preferred embodiment, when the limiting component 124 is in the second chute section V2 or the third chute section V3, it cannot slide back to the first chute section V1. In other words, the microfluidic detection device 1000 can switch from administrator mode to user mode, but cannot switch from user mode to administrator mode, thus preventing user error.

[0156] Although the embodiments of the present invention have been shown and described above, it will be understood that the above embodiments are illustrative and are not to be construed as limitations on the present invention. A person skilled in the art may change, modify, replace and modify the above embodiments within the scope of the present invention.

[0157] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of the technical features being referred to. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise specifically defined.

Claims

1. A microfluidic device, characterized in that: The microfluidic device includes a body and a flow channel switching component; The body is provided with a plurality of flow channels; The channel switching assembly is rotatably connected to the main body, and is provided with a liquid flow groove. The channel switching assembly is configured to be rotatable relative to the main body to adjust the position of the liquid flow groove so that at least two of the plurality of channels are connected via the liquid flow groove.

2. The microfluidic device according to claim 1, wherein The plurality of flow channels include a first flow channel, a second flow channel and a third flow channel; The liquid flow trough includes a first liquid flow trough, a second liquid flow trough and a third liquid flow trough; The flow channel switching assembly is configured to rotate to a first position so that the first liquid passage connects the first flow channel and the second flow channel; The flow channel switching assembly is configured to rotate to a second position so that the second liquid passage connects the second flow channel and the third flow channel, and the third liquid passage connects the first flow channel and the second flow channel.

3. The microfluidic device according to claim 2, wherein The plurality of flow channels further includes a fourth flow channel; The flow channel switching assembly is further configured to connect the second liquid passage to the third flow channel and the fourth flow channel when the second liquid passage is rotated to a third position.

4. The microfluidic device according to claim 2, wherein The first flow channel includes a second flow channel opening, the second flow channel includes a third flow channel opening and a fourth flow channel opening, the third flow channel includes a fifth flow channel opening and a sixth flow channel opening, and the second flow channel opening, the third flow channel opening, the fourth flow channel opening, the fifth flow channel opening and the sixth flow channel opening are arranged to overlap with the flow channel switching component in the upper and lower directions.

5. The microfluidic device according to claim 4, characterized in that The first flow channel further includes a first flow channel opening, and the first flow channel opening is located on one side of the flow channel switching component; The microfluidic device further includes a fluid storage component connected to the body and in communication with the first flow channel opening.

6. The microfluidic device according to claim 4, characterized in that The third flow channel further includes a seventh flow channel opening, which is located at one side of the flow channel switching component and is used to discharge the fluid in the third flow channel.

7. The microfluidic device according to claim 3, characterized in that The fourth flow channel includes an eighth flow channel opening and a ninth flow channel opening, and the eighth flow channel opening and the flow channel switching component are arranged to overlap in the upper and lower directions.

8. The microfluidic device according to claim 2, wherein: The third flow channel is a flow channel that extends in a circuitous manner and is used to collect fluid.

9. The microfluidic device according to claim 3, characterized in that The body is provided with a slide groove; The flow channel switching assembly includes a limiting component, which is configured to slide in the sliding groove during the rotation of the flow channel switching assembly relative to the body to enable the flow channel switching assembly to switch between the first position, the second position and the third position.

10. The microfluidic device according to claim 9, wherein: The slide groove includes a first slide groove section, a second slide groove section and a third slide groove section, and the limiting component is configured to slide along the first slide groove section to rotate the flow channel switching assembly to the first position, slide along the second slide groove section to rotate the flow channel switching assembly to the third position, and slide along the third slide groove section to rotate the flow channel switching assembly to the second position.

11. The microfluidic device according to claim 10, wherein: The chute is configured so that when the limiting component is located in the second chute section or the third chute section, it cannot slide to the first chute section.

12. The microfluidic device according to claim 10, wherein: The groove depth of the second chute section is equal to the groove depth of the third chute section, and the groove depths of the second chute section and the third chute section are greater than the groove depth of the first chute section.

13. The microfluidic device according to claim 10, wherein: The flow channel switching assembly further includes a rotating body and a sealing plate, wherein the rotating body is rotatably connected to the body, the sealing plate and the limiting component are both connected to the rotating body and can rotate with the rotating body, and the sealing plate is arranged in contact with the body; The first liquid flow channel, the second liquid flow channel, and the third liquid flow channel are arranged on the sealing plate.

14. The microfluidic device according to claim 1, wherein The body comprises an upper plate, a flow channel plate and a lower plate, and the upper surface and the lower surface of the flow channel plate are both provided with flow channel grooves; The upper plate and the lower plate are respectively arranged on the upper and lower sides of the flow channel plate to form a plurality of flow channels together with the flow channel grooves, and the plurality of flow channels include a second flow channel.

15. The microfluidic device according to claim 14, wherein: The plurality of flow channels further include a first flow channel and a fourth flow channel; The flow channel groove includes a first flow channel groove and a fourth flow channel groove, and the first flow channel groove and the fourth flow channel groove are arranged on the upper surface of the flow channel plate; The first flow channel groove and the upper plate together form the first flow channel, and the fourth flow channel groove and the upper plate together form the fourth flow channel.

16. The microfluidic device according to claim 14, wherein: The plurality of flow channels further includes a third flow channel; The flow channel groove includes a third flow channel groove, and the third flow channel groove includes a third flow channel groove first section, a third flow channel groove second section and a third flow channel groove third section; The first section of the third flow channel groove and the third section of the third flow channel groove are arranged on the upper surface of the flow channel plate, and the second section of the third flow channel groove is arranged on the lower surface of the flow channel plate; The first section of the third flow channel groove, the second section of the third flow channel groove, and the third section of the third flow channel groove are connected in sequence and form the third flow channel together with the upper plate and the lower plate.

17. The microfluidic device according to claim 14, wherein: The second flow channel includes a second flow channel first section and a second flow channel second section The flow channel groove includes a second flow channel groove, the second flow channel groove includes a second flow channel groove first section and a second flow channel groove second section, and the second flow channel groove first section and the second flow channel groove second section are arranged on the upper surface of the flow channel plate; The upper surface of the lower plate is provided with a first buffer groove and a second buffer groove; The first section of the second flow channel is connected to the first buffer tank, and together with the upper plate and the lower plate, forms the first section of the second flow channel; The second section of the second flow channel is connected to the second buffer tank, and together with the upper plate and the lower plate, forms the second section of the second flow channel; The first buffer tank is provided with a first liquid port, the second buffer tank is provided with a second liquid port, and the first section of the second flow channel and the second section of the second flow channel can communicate with each other through the first liquid port and the second liquid port.

18. The microfluidic device according to claim 2, wherein: The main body is provided with a transparent observation area, and the observation area is located above the second flow channel.

19. The microfluidic device according to claim 2, wherein: The plurality of flow channels include a second flow channel; The second flow channel is provided with a flow channel buffer zone, and the shape of the flow channel buffer zone is gradually increased and then gradually decreased along the extension direction of the second flow channel.

20. The microfluidic device according to claim 19, wherein The body is further provided with a sealed chamber, and the sealed chamber is located below the flow channel buffer zone; The microfluidic device also includes a membrane and a conductive sheet. The membrane is arranged between the sealed chamber and the flow channel buffer zone. The membrane is configured to allow ion transfer between the fluid in the sealed chamber and the fluid in the flow channel buffer zone. The conductive sheet is located below the membrane and is used to transmit signals to the outside.

21. The microfluidic device according to claim 20, wherein: The main body is further provided with a tenth flow channel opening and an eleventh flow channel opening, both of which are communicated with the sealed chamber and are used for injecting the detection liquid into the sealed chamber.

22. The microfluidic device according to claim 14, wherein: A liquid storage tank is provided on the lower surface of the flow channel plate; The flow channel groove includes a second flow channel groove second section, the second flow channel groove second section is provided with a flow channel buffer zone connected to the liquid storage tank, and the liquid storage tank is sealed by a membrane and the lower plate to form a sealed chamber; The membrane is arranged between the liquid storage tank and the flow channel buffer zone, and the lower plate is provided with a conductive sheet at a position overlapping with the liquid storage tank in the up-down direction.

23. A microfluidic detection device, characterized in that: The microfluidic detection device comprises a sensing device and a microfluidic device according to any one of claims 2 to 22; The sensing device is disposed at the second flow channel and connected to the body, and the sensing device and a corresponding portion of the body forming the second flow channel together form a sensing area.

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