Indocalamus seedling raising substrate and application thereof in indocalamus breeding

Abstract

The invention relates to the technical field of bamboo seedling raising, in particular to an indocalamus seedling raising substrate and application thereof in indocalamus breeding. The invention provides an indocalamus seedling raising substrate. The indocalamus seedling raising substrate comprises the following components in parts by volume: 5-7 parts of yellow subsoil, 1-3 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-1 part of bamboo ash. According to the indocalamus seedling raising substrate obtained by compounding the appropriate components, the bamboo shoot growing rate of indocalamus burial container seedling raising is increased, indocalamus can be rapidly bred, indocalamus seedlings are obtained, and compared with a control group, the indexes such as seedling height, leaf length and width, leaf area and total leaf number of the cultivated indocalamus seedlings are remarkably improved; growth of indocalamus seedlings is facilitated, and the requirements for large-scale planting and popularization of indocalamus in the future are met.

Description

Technical Field

[0001] The invention relates to the technical field of bamboo seedling cultivation, in particular to an indocalamus seedling cultivation substrate and application thereof in breeding indocalamus. Background Art

[0002] Indocalamus tessellatus is a perennial plant belonging to the genus Indocalamus of the subfamily Bambusoideae of the Poaceae family. Indocalamus leaves are natural green food packaging materials, often used for packaging food such as rice dumplings, cakes, and rice balls. They can also be used as cushions for tea, bamboo hats, and boat awnings. In addition, the content of flavonoids in bamboo leaves is high, and they have great development potential in drinking, eating, and medicinal purposes. In addition, Indocalamus tessellatus has a variety of habitats, is shade-tolerant, drought-tolerant, and barren-tolerant, and is a good project for the development of forest-based economic industries.

[0003] In recent years, with the continuous expansion of artificial bamboo planting area, the demand for bamboo seedlings has increased, and the quality requirements for seedlings have become higher and higher. However, the current breeding and planting of bamboo mainly rely on digging wild bamboo. This seedling raising method has a lot of uncertainties, high transportation costs, and low survival rate of long-distance transplanting, which is difficult to meet the current and future large-scale planting and promotion needs. Summary of the invention

[0004] In order to solve the above problems, the present invention provides a bamboo seedling cultivation substrate and its application in bamboo breeding. The bamboo seedling cultivation substrate provided by the present invention can improve the bamboo shoot germination rate and the accumulation of total biomass of the bamboo seedlings in the bamboo shoot buried seedling cultivation, realize the rapid breeding of bamboo seedlings in the bamboo shoot buried container, and meet the current and future bamboo scale planting and promotion needs.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] The invention provides a bamboo seedling culture medium, which comprises the following components in parts by volume: 5-7 parts of loess soil, 1-3 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-1 part of bamboo ash.

[0007] Preferably, the indocalamus seedling raising matrix comprises the following components in parts by volume: 5-6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-0.5 parts of bamboo ash.

[0008] Preferably, the indocalamus seedling raising matrix comprises the following components in parts by volume: 6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-0.5 parts of bamboo ash.

[0009] Preferably, the indocalamus seedling raising matrix comprises the following components in parts by volume: 6 parts of loess soil, 2 parts of peat soil and 1 part of bean cake fertilizer.

[0010] Preferably, the particle size of the loess soil is less than 50 mesh.

[0011] The invention provides a method for preparing the indocalamus seedling raising matrix described in the above technical solution, comprising: mixing various components in proportion to obtain the indocalamus seedling raising matrix.

[0012] The present invention provides the use of the indocalamus seedling raising substrate described in the above technical solution or the indocalamus seedling raising substrate prepared by the preparation method described in the above technical solution in breeding indocalamus.

[0013] The present invention provides a method for breeding indocalamus, comprising the following steps:

[0014] The bamboo rhizome of Indocalamus ovata is transversely buried in a container filled with a seedling culture medium to cultivate Indocalamus ovata seedlings; the seedling culture medium is the Indocalamus ovata seedling culture medium described in the above technical solution or the Indocalamus ovata seedling culture medium prepared by the preparation method described in the above technical solution.

[0015] Preferably, the length of the bamboo whip of Indocalamus truncatum is 8 to 12 cm; the bamboo whip of Indocalamus truncatum is the underground bamboo whip of Indocalamus truncatum and / or the whip section with full bamboo shoots in the middle of the bamboo whip.

[0016] Preferably, the culture conditions include: a temperature of 18 to 22° C.; and a humidity of 40% to 80% of the seedling culture medium.

[0017] Beneficial effects:

[0018] The present invention provides a bamboo seedling medium, comprising the following components by volume: 5-7 parts of loess soil, 1-3 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-1 part of bamboo ash. The bamboo seedling medium obtained by compounding suitable components in the present invention not only improves the bamboo shoot growth rate of bamboo shoots buried in a container, but also can quickly breed bamboo to obtain bamboo seedlings. Moreover, the cultivated bamboo seedlings are significantly improved in terms of seedling height, leaf length and width, leaf area and total leaf number compared with a control group, which is beneficial to the growth of bamboo seedlings and meets the future demand for large-scale planting and promotion of bamboo. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0020] Figure 1 Root length of Indocalamus japonica seedlings in containers treated with different seedling mediums;

[0021] Figure 2 The number of primary roots of Indocalamus japonica seedlings in containers treated with different seedling mediums;

[0022] Figure 3 The biomass of Indocalamus japonica seedlings in containers treated with different seedling mediums;

[0023] Figure 4Biomass distribution ratio of Indocalamus japonica container seedlings treated with different seedling raising media. DETAILED DESCRIPTION

[0024] The invention provides a bamboo seedling culture medium, which comprises the following components in parts by volume: 5-7 parts of loess soil, 1-3 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-1 part of bamboo ash.

[0025] Unless otherwise specified, the present invention has no special requirements on the sources of the components of the indocalamus seedling raising matrix, and commercially available products known to those skilled in the art may be used.

[0026] As an embodiment, the indocalamus seedling raising matrix comprises the following components by volume: 5-6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer, and 0-0.5 parts of bamboo ash. As another embodiment, the indocalamus seedling raising matrix comprises the following components by volume: 6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer, and 0-0.5 parts of bamboo ash. As another embodiment, the indocalamus seedling raising matrix comprises the following components by volume: 6 parts of loess soil, 2 parts of peat soil, and 1 part of bean cake fertilizer.

[0027] As an embodiment, the yellow heart soil of the present invention is soil in a bamboo plant germplasm garden; as another embodiment, the preparation method of the yellow heart soil comprises: after digging out the soil in the bamboo plant germplasm garden, removing impurities, and then crushing the soil after removing impurities through a 50-mesh molecular sieve, collecting the sieve under the yellow heart soil; the impurities include fallen leaves, rotten branches and mosses. The present invention selects the yellow heart soil as the cultivation medium, which can not only use local materials in the bamboo plant germplasm garden to save costs, but also the yellow heart soil is light in texture, rich in nitrogen, phosphorus, potassium and other components, and is suitable for the growth of indigo bamboo.

[0028] As an implementation manner, the bamboo ash is ashes obtained by burning waste residues of bamboo plants.

[0029] As an embodiment, the peat soil and bean cake fertilizer are purchased online, wherein the peat soil is purchased from Taobao store Aimei Gardening, and the bean cake fertilizer is purchased from Jingdong store Herun Gardening Store. Peat soil has a fine texture, strong water and fertilizer retention capacity, and can increase the permeability of the matrix; bean cake fertilizer can be used as a fertilizer, rich in nitrogen, phosphorus, potassium and other trace elements, and has little effect on the soil pH after fertilization, and can improve the soil shape.

[0030] The indocalamus seedling cultivation substrate obtained by compounding suitable components of the present invention not only improves the bamboo shoot germination rate of indocalamus seedling cultivation in an indocalamus-buried whip container, but can quickly breed indocalamus to obtain indocalamus seedlings. Moreover, the cultivated indocalamus seedlings are significantly improved in terms of seedling height, leaf length and width, leaf area, total leaf number and other indicators compared with a control group, which is beneficial to the growth of indocalamus seedlings and meets the future demand for large-scale planting and promotion of indocalamus.

[0031] The invention provides a method for preparing the indocalamus seedling raising matrix described in the above technical solution, comprising: mixing various components in proportion to obtain the indocalamus seedling raising matrix.

[0032] Based on the above advantages, the present invention provides the use of the indocalamus seedling raising substrate described in the above technical solution or the indocalamus seedling raising substrate prepared by the preparation method described in the above technical solution in breeding indocalamus.

[0033] The present invention provides a method for breeding indocalamus, comprising the following steps:

[0034] The bamboo rhizome of Indocalamus ovata is transversely buried in a container filled with a seedling culture medium to cultivate Indocalamus ovata seedlings; the seedling culture medium is the Indocalamus ovata seedling culture medium described in the above technical solution or the Indocalamus ovata seedling culture medium prepared by the preparation method described in the above technical solution.

[0035] As an implementation mode, the length of the bamboo whip of Indocalamus truncatum is 8 to 12 cm; the bamboo whip of Indocalamus truncatum is the underground bamboo whip of Indocalamus truncatum and / or the whip section with full bamboo shoots in the middle of the bamboo whip.

[0036] As an embodiment, the culture conditions include: a temperature of 18 to 22°C; and a humidity of the seedling medium of 40% to 80%. As another embodiment, the culture period is most suitable in spring, with a temperature of 18 to 22°C. After the bamboo shoots of Indocalamus are horizontally buried in a container containing the seedling medium, the seedling medium is watered thoroughly. Before the seedlings grow out, water is frequently applied. On sunny days, when the temperature is high, water is applied once every 4 to 5 days, and the container is kept at a certain humidity. On cloudy or rainy days, when the temperature is low, water is applied once every 7 to 10 days. After the Indocalamus seedlings sprout and grow, the number of waterings is reduced, and water is added when the soil in the container is relatively dry and the soil humidity is low.

[0037] In order to further illustrate the present invention, a bamboo seedling raising substrate provided by the present invention and its application in breeding bamboo are described in detail below in conjunction with the accompanying drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0038] Preparation Example

[0039] The sources of the components in the following embodiments are as follows: loess is soil in a bamboo plant germplasm garden. After digging it out, some impurities such as fallen leaves, rotten branches, mosses, etc. are removed, and then the clean loess is crushed through a 50-mesh molecular sieve to form fine loess; peat soil and bean cake fertilizer are general soil fertilizers purchased online, peat soil is purchased from Taobao store Aimei Gardening, and bean cake fertilizer is purchased from JD store Herun Gardening Shop; bamboo ash is the ashes of the waste residues of bamboo plants in the broom base in Shuangfeng Town, Yifeng County, Jiangxi Province, and the unburned bamboo stalks, bamboo leaves and other wastes are removed to leave clean bamboo ash.

[0040] Example 1

[0041] A seedling culture medium for indocalamus (recorded as JZ1) is composed of the following components in parts by volume: 5 parts of loess soil, 3 parts of peat soil and 2 parts of bean cake fertilizer.

[0042] Example 2

[0043] A seedling culture medium for Indocalamus truncatula (referred to as JZ2) is composed of the following components in parts by volume: 5 parts of loess soil, 2 parts of peat soil, 1.5 parts of bean cake fertilizer and 0.5 parts of bamboo ash.

[0044] Example 3

[0045] A seedling culture medium for Indocalamus truncatula (referred to as JZ3) is composed of the following components in parts by volume: 5 parts of loess soil, 1 part of peat soil, 1 part of bean cake fertilizer and 1 part of bamboo ash.

[0046] Example 4

[0047] A seedling culture medium for Indocalamus truncatula (referred to as JZ4) is composed of the following components in parts by volume: 6 parts of loess soil, 3 parts of peat soil, 1.5 parts of bean cake fertilizer and 1 part of bamboo ash.

[0048] Example 5

[0049] A seedling culture medium for indocalamus (recorded as JZ5) is composed of the following components in parts by volume: 6 parts of loess soil, 2 parts of peat soil and 1 part of bean cake fertilizer.

[0050] Example 6

[0051] A seedling culture medium for Indocalamus truncatula (referred to as JZ6) is composed of the following components in parts by volume: 6 parts of loess soil, 1 part of peat soil, 2 parts of bean cake fertilizer and 0.5 part of bamboo ash.

[0052] Example 7

[0053] A seedling culture medium for Indocalamus truncatula (referred to as JZ7) is composed of the following components in parts by volume: 7 parts of loess soil, 3 parts of peat soil, 1 part of bean cake fertilizer and 0.5 part of bamboo ash.

[0054] Example 8

[0055] A seedling culture medium for Indocalamus truncatula (recorded as JZ8) is composed of the following components in parts by volume: 7 parts of loess soil, 2 parts of peat soil, 2 parts of bean cake fertilizer and 1 part of bamboo ash.

[0056] Example 9

[0057] A seedling culture medium for indocalamus (recorded as JZ9) is composed of the following components in parts by volume: 7 parts of loess soil, 1 part of peat soil and 1.5 parts of bean cake fertilizer.

[0058] Test Case

[0059] 1.1 Overview of the test site

[0060] The test site is located in Jiaoqiao Town, Economic and Technological Development Zone, Nanchang City, Jiangxi Province, at 115°49′56″ east longitude, 28°45′54″ north latitude, and 841.4m above sea level. The region has a subtropical humid monsoon climate with four distinct seasons, relatively short spring and autumn, and relatively long summer and winter. The annual average temperature is 19.5℃, and the annual average relative humidity is 78.5%.

[0061] 1.2 Test materials

[0062] Indocalamus tessellatus (Munro) PCKeng in the Bamboo Garden of Jiangxi Agricultural University was selected as the experimental material. Bamboo rhizomes with good growth, plump buds and buds were selected as container seedling materials, and the experiment was carried out at the end of March 2024.

[0063] 1.3 Container Selection

[0064] In this experiment, black plastic nutrient pots were selected as the containers for raising seedlings of Indocalamus chinensis. According to the growth characteristics of Indocalamus chinensis and practical principles, black plastic nutrient pots with a diameter of 21 cm × 21 cm were selected.

[0065] 1.4 Bamboo whip selection

[0066] Select underground bamboo rhizomes of Indocalamus or the rhizomes with full shoots in the middle of the rhizomes, and cut them into 8-12 cm (including 4-5 nodes) lengths with pruning shears. The cuts should be smooth and have a certain root system and rhizosphere soil. Select healthy bamboo rhizomes with no diseases or insect pests, no damage, and with side buds, and store them in plastic bags to keep them moist for later use.

[0067] 1.5 Proportion of matrix

[0068] The seedling substrates of Examples 1 to 9 were mixed in proportion and loaded into a bamboo seedling container (the loading amount was 3 / 4 of the volume of the bamboo seedling container) for container seedling cultivation. At the same time, a control group (CK) made entirely of loess soil was set up, with 30 pots of container seedlings in each treatment, for a total of 300 pots of container seedlings.

[0069] 1.6 Data measurement and analysis

[0070] The experiment of seedling cultivation in container with buried whip of Indocalamus started at the end of March 2024. Each treated seedling matrix test group was placed in a greenhouse in the bamboo germplasm garden of Jiangxi Agricultural University for seedling cultivation. After 3 months, the shoot growth rate of each treatment of Indocalamus buried whip seedling was counted. The experimental data was measured in mid-November 2024. Five seedlings were selected for each treatment to measure the seedling height (H), ground diameter (D0), leaf length and width, leaf area, etc. After washing the seedlings with different treatments and drying them with absorbent paper, the root length and fresh weight of roots, stems and leaves were measured. Indoor measurement, the roots, stems and leaves were put into envelopes respectively, fixed at 105℃ for 30min, dried at 65℃ until the mass was constant, and the dry matter mass of each part was measured. The data were statistically calculated and analyzed by Excel 2021 and Spss 26.0 for significant test and correlation analysis, and the graphics were drawn by Origin2024 to test the effect of seedling matrix of different embodiments on the growth of Indocalamus container seedlings.

[0071] 1.6.1 The test results of seedling height, ground diameter, number of ramets and shoot growth rate of Indocalamus chinensis seedlings in different seedling culture media are shown in Table 1.

[0072] Table 1 Seedling height, ground diameter, number of ramets and shoot growth rate of Indocalamus chinensis seedlings in different seedling culture media

[0073] serial number H(cm) <![CDATA[D0(cm)]]> Number of ramets Bamboo shoot growth rate (%) <![CDATA[JZ1]]> 13.30±1.79cd 0.21±0.03 2.60±0.89cd 50.0 <![CDATA[JZ2]]> 18.54±4.18abc 0.22±0.06 3.60±1.67bcd 67.7 <![CDATA[JZ3]]> 12.48±3.02d 0.18±0.04 3.40±1.14bcd 23.3 <![CDATA[JZ4]]> 18.64±4.16ab 0.21±0.02 2.80±0.84cd 67.7 <![CDATA[JZ5]]> 20.32±5.12a 0.23±0.04 6.20±3.96a 90.0 <![CDATA[JZ6]]> 18.92±4.46ab 0.22±0.02 5.60±2.07ab 63.3 <![CDATA[JZ7]]> 17.24±3.59abcd 0.20±0.04 2.60±0.89cd 56.7 <![CDATA[JZ8]]> 17.14±2.58abcd 0.22±0.01 4.00±1.58abc 33.3 <![CDATA[JZ9]]> 15.04±1.05abcd 0.22±0.04 2.20±0.84cd 56.7 CK 14.64±4.40bcd 0.17±0.04 1.40±0.55d 36.7

[0074] Note: The data in the table are Duncan analysis: mean ± standard deviation, different letters indicate significant differences at the 5% level, and the same applies to the following tables.

[0075] The matrix is ​​the carrier for the growth of container seedlings, and its composition and ratio will directly affect the growth state of the seedlings. The statistical results of the experimental data showed that the influence of different seedling substrates on the growth characteristics of the aboveground part of the bamboo reached a significant level (P=0.05). As shown in Table 1, except for the two treatments JZ3 and JZ1, the height of the bamboo seedlings was smaller than that of the control group, the height of the bamboo seedlings in the other treatments was significantly greater than that of the control group, and the ground diameter and the number of ramets of the bamboo seedlings in all treatments were greater than those of the control group. The highest height of the bamboo seedlings was in treatment JZ5, reaching 20.32cm, which was 38.80% higher than CK's 14.64, followed by treatment JZ6, with a seedling height of 18.92cm, which was 29.23% higher than CK. The ground diameter of the bamboo seedlings was not much different, among which the largest ground diameter was in treatment JZ5, reaching 0.23cm, which was 35.29% higher than CK's 0.17cm. The treatment with the largest number of ramets of Indocalamus spp. was JZ5, reaching 6.20 ramets, which was 440% of CK's 1.40 ramets, followed by JZ6, with an average of 5.60 ramets, which was 400% of CK. Through Duncan analysis, it was found that the treatment with the highest seedling height, the largest ground diameter, the largest number of ramets and the highest shoot rate of Indocalamus spp. was JZ5. It can be seen that treatment JZ5 can not only provide Indocalamus spp. with more sufficient nutrients, but also provide more ideal physical and chemical properties, which can meet the growth needs of Indocalamus spp. during the nursery period and has the greatest growth-promoting effect on Indocalamus spp.

[0076] 1.6.2 The results of the effects of different seedling media on the leaf index growth of Indocalamus japonicus container seedlings are shown in Table 2.

[0077] Table 2 Leaf length, width, leaf area and total number of leaves of Indocalamus chinensis seedlings in different seedling mediums

[0078] serial number Leaf width(cm) Leaf length(cm) <![CDATA[Leaf area (cm 2 )]]> Total number of blades <![CDATA[JZ1]]> 2.08±0.38bc 11.27±1.68cde 18.87±8.92c 14.20±4.09cd <![CDATA[JZ2]]> 2.16±0.50bc 12.53±2.84cde 21.72±12.96bc 17.00±5.10bcd <![CDATA[JZ3]]> 1.65±0.30c 9.24±1.50e 10.55±4.78c 16.40±6.84bcd <![CDATA[JZ4]]> 2.56±0.56ab 15.00±3.52abc 37.49±16.30ab 13.60±3.71cd <![CDATA[JZ5]]> 2.88±0.28a 18.59±2.78a 50.14±4.99a 29.40±5.46a <![CDATA[JZ6]]> 2.48±0.32ab 16.61±1.62ab 22.77±8.52bc 23.00±7.91ab <![CDATA[JZ7]]> 2.09±0.50bc 12.51±2.90cde 22.57±21.35bc 14.60±3.21bcd <![CDATA[JZ8]]> 2.13±0.45bc 13.33±3.20bcd 23.66±5.55bc 18.20±11.69bc <![CDATA[JZ9]]> 2.17±0.56bc 13.89±4.31bcd 21.86±12.39bc 10.60±4.04cd CK 1.95±0.58bc 10.83±2.57de 12.05±4.10c 9.00±1.41d

[0079] Leaves are an important part of plants. Data analysis of plant leaves in terms of length, width, area and total number of leaves can be used to evaluate and observe the growth status of plants. Statistical data analysis of leaf length, width, leaf area and leaf number of Indocalamus chinensis seedlings was performed. The results showed that the total number of leaves, leaf length and leaf area of ​​Indocalamus chinensis seedlings in different seedling media reached extremely significant levels (P=0.01), while the leaf width reached significant levels (P=0.05). As shown in Table 3, the largest leaf length and width of Indocalamus chinensis seedlings was in treatment JZ5, reaching 2.88 cm and 18.59 cm respectively. The leaf width was 47.69% higher than CK's 1.95 cm, and the leaf length was 71.65% higher than CK's 10.83 cm. The second largest leaf width was in treatment JZ4, reaching 2.56 cm, 31.28% higher than CK, and the largest leaf length was in treatment JZ6, reaching 16.61 cm, 53.37% higher than CK. The treatment with the largest leaf area and total leaf number was JZ5, which were 50.14 cm 2The average number of leaves is 29.40, and the leaf area is 316.10% higher than that of CK (12.05 cm²). The total number of leaves is 226.67% more than that of CK (9.00). It can be seen that the leaf length, width, leaf area, and total number of leaves of the Indocalamus tessellatus seedlings treated with JZ5 are the largest, indicating that the growth condition of the Indocalamus tessellatus seedlings treated with JZ5 is the best, and this treatment substrate is the most suitable for the growth of Indocalamus tessellatus seedlings. 2 The leaf length, width, leaf area, and total number of leaves of the Indocalamus tessellatus seedlings treated with JZ5 are the largest, indicating that the growth condition of the Indocalamus tessellatus seedlings treated with JZ5 is the best, and this treatment substrate is the most suitable for the growth of Indocalamus tessellatus seedlings.

[0080] 1.6.3 Effects of different seedling-raising substrates on the root growth of Indocalamus tessellatus container seedlings buried with rhizomes are shown in Figure 1 and Figure 2 .

[0081] In addition to supporting the whole plant, the most important function of the plant roots is to absorb water and nutrients from the surrounding environment. Figure 1 There are extremely significant differences in root length and the number of primary roots among the substrate ratio treatments in Figure 2 , with Sig values of 0.000 and 0.000 respectively. The longest root length is JZ5, reaching 38.92 cm, followed by JZ6 with a root length of 21.92 cm, which are 592.53% and 289.68% higher than the lowest JZ9 respectively. The other root lengths are in the order of JZ2 < JZ8 < JZ1 < JZ7 < JZ3 < JZ4 < CK. The largest number of primary roots is JZ5, reaching 30.40, followed by JZ6 with 21.00 roots, which are 375% and 228.13% more than the least JZ2 respectively. The other numbers of roots are in the order of JZ9 < JZ1 < CK < JZ7 < JZ3 < JZ4 < JZ8. Considering the underground root length and the number of primary roots comprehensively, it shows that the seedling-raising substrate JZ5 is more conducive to the underground root growth of Indocalamus tessellatus seedlings and is more beneficial to improving the root absorption and growth ability of Indocalamus tessellatus seedlings.

[0082] 1.6.4 Effects of different seedling-raising substrates on the biomass and biomass distribution of Indocalamus tessellatus container seedlings buried with rhizomes are shown in Figure 3 and Figure 4 .

[0083] The size and distribution of biomass are important indicators for evaluating the quality of seedlings. From Figure 3 and Figure 4It can be seen that there are extremely significant differences in the biomass of Indocalamus tessellatus seedlings among different seedling-raising substrates. The highest biomass is JZ5, which is 543.60% higher than CK and 1134.55% higher than JZ2 with the lowest biomass. JZ6 and JZ8 follow next, and there are little differences in biomass among the remaining 5 treatments, which are at the same biomass level. The biomass distribution in roots, rhizomes and leaves is root > leaf > stem, with the distribution ratios being 50.17%, 30.66% and 19.17% respectively. Among different seedling-raising substrates, the highest biomass distribution in roots, rhizomes and leaves for each treatment is JZ5, with 4.94 g in roots, 3.48 g in stems and 5.16 g in leaves respectively. From the changes in biomass and its distribution, it can be concluded that the seedling-raising substrate JZ5 is more conducive to the growth of Indocalamus tessellatus seedlings. Among the seedling-raising substrates, the treatments with yellow heart soil accounting for 52.17% - 66.67% mainly and peat soil accounting for 10.53% - 26.09% as a supplement have the best biomass and its distribution, indicating that yellow heart soil and peat soil under this seedling-raising substrate are more conducive to the accumulation of biomass of Indocalamus tessellatus seedlings.

[0084] 1.6.5 The results of the effects of different seedling-raising substrates on the seedling quality index of Indocalamus tessellatus container seedlings with buried rhizomes are shown in Table 3.

[0085] Table 3 Height-stem ratio, root-rhizome ratio and total dry mass of Indocalamus tessellatus seedlings with different seedling-raising substrates

[0086] serial number High stem ratio Root-to-shoot ratio Total dry mass (g) <![CDATA[JZ1]]> 79.25±10.94c 5.20±2.71b 1.96±0.86cd <![CDATA[JZ2]]> 86.35±19.84abc 2.45±3.49b 1.1±0.95d <![CDATA[JZ3]]> 69.52±9.23bc 7.31±6.26b 2.54±1.00bcd <![CDATA[JZ4]]> 88.23±13.74ab 1.86±0.46b 2.88±1.58bcd <![CDATA[JZ5]]> 65.85±13.25c 1.49±0.36b 13.58±2.94a <![CDATA[JZ6]]> 70.23±10.10abc 3.93±2.92b 4.56±1.82b <![CDATA[JZ7]]> 81.08±16.79abc 2.32±3.28b 2.42±2.14bcd <![CDATA[JZ8]]> 91.35±13.62abc 2.65±1.67b 3.84±2.63bc <![CDATA[JZ9]]> 85.53±23.58a 16.07±13.14a 2.68±1.00bcd CK 87.23±22.08abc 7.56±2.81b 2.11±0.24bcd

[0087] The seedling quality index is obtained by integrating several indicators and comprehensively reflects the quality of the seedlings. The lower the height-diameter ratio of the seedlings, the stronger the above-ground part of the seedlings, which can improve the resistance of the seedlings; the smaller the root-rhizome ratio, the larger the proportion of the underground part of the seedlings, resulting in higher transplanting survival rate of the seedlings, shorter seedling slowdown period and faster later growth; at the same seedling age, the larger the total dry mass, the more organic matter contained in the seedlings and the better the seedling quality. Therefore, among these three indicators, the smaller the height-diameter ratio and root-rhizome ratio, and the larger the total dry mass, the higher the seedling quality index and the better the seedling quality. As can be seen from Table 3, the differences in height-diameter ratio and root-rhizome ratio among different treatments reached a significant level (P = 0.05), while the differences in total dry mass among different treatments reached an extremely significant level (P = 0.01). The height-diameter ratio, from small to large, is JZ5 < JZ3 < JZ6 < JZ1 < JZ7 < JZ9 < JZ2 < CK < JZ4 < JZ8; there are certain differences in the root-rhizome ratio, from small to large, which are JZ5 < JZ4 < JZ7 < JZ2 < JZ8 < JZ6 < JZ1 < JZ3 < CK < JZ9. The total dry mass is the biomass, the highest biomass is JZ5 = 13.58 g, followed by JZ6 with a biomass of 4.56 g. The lowest biomass is JZ2 with a biomass of 1.1 g. The seedling quality index is obtained from the above three indicators, and the best is the seedling-raising substrate treatment JZ5, with yellow heart soil:peat soil:bean cake fertilizer:bamboo ash = 6:2:1:0.

[0088] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A bamboo seedling culture medium, characterized in that: The invention comprises the following components in parts by volume: 5-7 parts of loess soil, 1-3 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-1 part of bamboo ash.

2. The indocalamus seedling raising matrix according to claim 1, characterized in that: The indocalamus seedling raising matrix comprises the following components in parts by volume: 5-6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-0.5 parts of bamboo ash.

3. The indocalamus seedling raising matrix according to claim 2, characterized in that: The indocalamus seedling raising matrix comprises the following components in parts by volume: 6 parts of loess soil, 1-2 parts of peat soil, 1-2 parts of bean cake fertilizer and 0-0.5 parts of bamboo ash.

4. The indocalamus seedling raising matrix according to claim 3, characterized in that: The indocalamus seedling raising matrix comprises the following components in parts by volume: 6 parts of loess soil, 2 parts of peat soil and 1 part of bean cake fertilizer.

5. The indocalamus seedling raising substrate according to any one of claims 1 to 4, characterized in that: The particle size of the loess soil is less than 50 meshes.

6. The method for preparing the indocalamus seedling culture medium according to any one of claims 1 to 5, characterized in that: include: Various components are mixed in proportion to obtain the indocalamus seedling raising medium.

7. Use of the indocalamus seedling raising substrate according to any one of claims 1 to 5 or the indocalamus seedling raising substrate prepared by the preparation method according to claim 6 in breeding indocalamus.

8. A method for breeding Indocalamus truncatula, characterized in that: The following steps are involved: The bamboo rhizome of Indocalamus ovata is transversely buried in a container containing a seedling culture medium to cultivate Indocalamus ovata seedlings; the seedling culture medium is the Indocalamus ovata seedling culture medium according to any one of claims 1 to 5 or the Indocalamus ovata seedling culture medium prepared by the preparation method according to claim 6.

9. The method according to claim 8, characterized in that The length of the bamboo whip of the indocalamus is 8 to 12 cm; the bamboo whip of the indocalamus is the underground bamboo whip of the indocalamus and / or the whip section with full bamboo shoots in the middle of the bamboo whip.

10. The method according to claim 8, characterized in that The culture conditions include: the temperature is 18-22° C.; the humidity of the seedling culture medium is 40%-80%.

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