Method for controlling blood processing chip

Abstract

The invention discloses a control method of blood processing chips. The blood processing chips are loaded in a centrifugal machine; upper plasma and red blood cells are separated based on first centrifugation of blood, and normal saline enters a mixing area; the centrifugal machine stops working, the red blood cells sequentially enter a siphon flow channel and sequentially trigger second-time centrifugation and third-time centrifugation, the siphon flow channel is in an open state, and the red blood cells enter the mixing area from the red blood cell quantifying area; the red blood cells are layered with normal saline in the mixing area due to centrifugal action; carrying out fourth centrifugation, carrying out positive and negative rotation of the centrifugal machine, carrying out re-uniform mixing through oscillation, carrying out red blood cell resuspension, and carrying out multiple times of separation on the blood along the vertical direction; rotating the centrifugal tray by 90 degrees, standing for 3 minutes, and enabling the resuspension in the uniform mixing area to enter a capillary quantitative flow channel; the blood processing chip is matched with the detection card and judges the blood type, and the blood sample, the normal saline and the reverse red blood cells are located in the blood processing chip and are separated for multiple times in the blood processing chip.

Description

Technical Field

[0001] The present invention relates to the technical field of blood processing chips, and in particular to a control method of a blood processing chip. Background Art

[0002] With the development of science and technology, blood processing chips are used for blood type determination, and are combined with detection cards for determination, so as to determine the blood type through the agglutination reaction of the detection card. In the prior art, the blood processing chip and the detection card are docked in a horizontal direction, and the separation channel in the blood processing chip flows in a horizontal direction, and the blood flows in a horizontal direction under the action of a pump body and is gradually separated, which affects the coordination effect of the existing blood processing chip and the detection card. Summary of the invention

[0003] The purpose of the present invention is to overcome the shortcomings of the prior art. The present invention provides a control method for a blood processing chip. A blood sample, physiological saline and anti-fixed red blood cells are in the blood processing chip and are separated multiple times in the blood processing chip to facilitate multiple separations of the blood sample, physiological saline and anti-fixed red blood cells. At the same time, the blood is separated multiple times in the vertical direction, and the blood processing chip cooperates with a detection card to realize blood type determination, thereby improving the coordination effect between the blood processing chip and the detection card. At this time, the blood processing chip is separated in the vertical direction, so that the centrifuge is compatible with multiple blood processing chips for synchronous separation, and the circumferential space of the centrifuge is effectively utilized.

[0004] In order to solve the above technical problems, an embodiment of the present invention provides a control method of a blood processing chip, characterized in that it is applied to a blood processing chip;

[0005] The control method of the blood processing chip comprises:

[0006] Adding blood samples, physiological saline and anti-red blood cells to blood processing chips, wherein each blood processing chip is loaded into a centrifuge;

[0007] Based on the first centrifugation of blood, plasma and red blood cells are separated, and saline enters the mixing zone;

[0008] The centrifuge stops working, and the red blood cells enter the siphon flow channel in turn, triggering the second centrifugation and the third centrifugation in turn, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect;

[0009] In the fourth centrifugation, the centrifuge rotates forward and reversely, remixes by oscillation, and the red blood cells are resuspended. During this process, the blood is separated multiple times in the vertical direction.

[0010] The centrifugal tray rotates 90 degrees and stands for 3 minutes, and the resuspended liquid in the mixing area enters the capillary quantitative flow channel;

[0011] The blood processing chip cooperates with the test card to determine the blood type.

[0012] Optionally, the blood sample, physiological saline and anti-red blood cells are added to the blood processing chip, wherein each blood processing chip is loaded into a centrifuge, including:

[0013] The blood sample is added to the blood feeding area of ​​the blood processing chip, wherein the plasma separation area is located below the blood feeding area;

[0014] Physiological saline is added to the physiological saline feeding area of ​​the blood processing chip, and the physiological saline feeding area is on the right side of the blood feeding area;

[0015] The anti-fixed red blood cells are added to the anti-fixed red blood cell feeding area of ​​the blood processing chip, and the anti-fixed red blood cell feeding area is located at the lower side of the physiological saline feeding area.

[0016] Optionally, the first centrifugation of blood to separate plasma and red blood cells and the physiological saline entering the mixing zone comprises:

[0017] The blood is centrifuged for the first time, and the blood flows vertically downward from the blood feeding area to the plasma separation area and the red blood cell separation area, and the upper plasma and red blood cells are separated; wherein the centrifugation parameters are 2000rpm and 4mins;

[0018] Physiological saline is separated synchronously and enters the mixing zone in sequence.

[0019] Optionally, the centrifuge stops working, the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, including:

[0020] After the centrifuge stops working, the red blood cells enter the siphon flow channel one by one and trigger the siphon valve of the siphon flow channel.

[0021] Optionally, the centrifuge stops working, the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, and further comprising:

[0022] In the second centrifugation, the centrifugation parameters were 1000 rpm and 2 mins, and the siphon valve was in the open state;

[0023] Most of the plasma enters the plasma quantification area from the plasma separation area, and the excess plasma enters the plasma waste liquid area;

[0024] Most of the red blood cells move from the red blood cell separation area to the red blood cell buffer area and the red blood cell quantification area under the siphon effect of the siphon flow channel, and the excess red blood cells enter the red blood cell waste liquid area.

[0025] Optionally, the centrifuge stops working, the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, and further comprising:

[0026] The centrifuge is paused, the siphon channel of the red blood cell buffer is filled, and the third separation is triggered;

[0027] In the third centrifugation, the centrifugation parameters were 1000 rpm and 2 mins, and the siphon valve was in the open state;

[0028] The red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect.

[0029] Optionally, in the fourth centrifugation, the centrifuge rotates forward and reversely, remixes by oscillation, and the red blood cells are resuspended, wherein the blood is separated multiple times in the vertical direction, including:

[0030] In the fourth centrifugation, the centrifuge rotates forward and reversely, and further mixes the red blood cells and saline in the mixing area;

[0031] The red blood cells are resuspended by remixing through vortexing, wherein the blood is separated multiple times in the vertical direction.

[0032] Optionally, the centrifugal tray is rotated 90 degrees and left to stand for 3 minutes, and the resuspended liquid in the mixing zone enters the capillary quantitative flow channel, including:

[0033] The centrifuge tray was rotated 90 degrees and allowed to stand for 3 minutes;

[0034] The resuspended liquid in the mixing area enters the capillary quantitative flow channel and is transferred from the front side of the blood processing chip to the back side of the blood processing chip.

[0035] Optionally, the blood processing chip cooperates with a detection card to determine the blood type, including:

[0036] Tear off the sealed aluminum foil, load the blood processing chip onto the test card according to the marked direction, and connect it through the interference fit buckle.

[0037] Optionally, the blood processing chip cooperates with a detection card to determine the blood type, and further includes:

[0038] Release all the liquid in the quantitative area into the test card, and centrifuge the liquid in the test card into the microcolumn gel; determine the blood type by comparing the agglutination reaction of the test card, wherein the test card is located below the blood processing chip and is connected to the blood processing chip.

[0039] In an embodiment of the present invention, a blood sample, saline and anti-red blood cells are added to a blood processing chip by the method in the embodiment of the present invention, wherein each blood processing chip is loaded into a centrifuge; based on the first centrifugation of the blood, upper plasma and red blood cells are separated, and saline enters the mixing zone; the centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the saline in the mixing zone due to the centrifugal effect; in the fourth centrifugation, the centrifuge is rotated forward and reversed, and the red blood cells are re-mixed by oscillation, and the blood is resuspended, wherein the blood is separated multiple times along the vertical direction; the centrifuge The centrifugal tray is rotated 90 degrees and left to stand for 3 minutes, and the heavy suspension in the mixing area enters the capillary quantitative flow channel; the blood processing chip cooperates with the detection card to determine the blood type, wherein the blood sample, physiological saline and anti-fixed red blood cells are in the blood processing chip, and multiple separations are performed in the blood processing chip to facilitate multiple separations of the blood sample, physiological saline and anti-fixed red blood cells. At the same time, the blood is separated multiple times in the vertical direction, and the blood processing chip cooperates with the detection card to realize the determination of the blood type, thereby improving the coordination effect between the blood processing chip and the detection card. At this time, the blood processing chip is separated in the vertical direction, so that the centrifuge is compatible with multiple blood processing chips for synchronous separation, and the circumferential space of the centrifuge is effectively utilized. BRIEF DESCRIPTION OF THE DRAWINGS

[0040] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0041] Figure 1 is a flow chart of a control method of a blood processing chip in an embodiment of the present invention;

[0042] Figure 2 is a flow chart of S12 in the control method of the blood processing chip in the embodiment of the present invention;

[0043] Figure 3 is a schematic flow chart of S13 in the control method of the blood processing chip in the embodiment of the present invention;

[0044] Figure 4 is a flow chart of S14 in the control method of the blood processing chip in the embodiment of the present invention;

[0045] Figure 5 is a flow chart of S15 in the control method of the blood processing chip in the embodiment of the present invention;

[0046] Figure 6 It is a schematic diagram of the structure of the control method of the blood processing chip in the embodiment of the present invention. DETAILED DESCRIPTION

[0047] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] Example

[0049] See also Figures 1 to 6 , a control method of a blood treatment chip, applied to a blood treatment chip; the control method of a blood treatment chip comprises:

[0050] Step S11: adding a blood sample, physiological saline and anti-red blood cells to a blood processing chip, wherein each blood processing chip is loaded into a centrifuge;

[0051] Step S12: The upper plasma and red blood cells are separated based on the first centrifugation of the blood, and the physiological saline enters the mixing area;

[0052] Step S13: the centrifuge stops working, the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect;

[0053] Step S14: the fourth centrifugation, the centrifuge rotates forward and reversely, remixes by oscillation, and the red blood cells are resuspended, wherein the blood is separated multiple times in the vertical direction;

[0054] Step S15: the centrifugal tray is rotated 90 degrees and allowed to stand for 3 minutes, and the resuspended liquid in the mixing zone enters the capillary quantitative flow channel;

[0055] Step S16: The blood processing chip cooperates with the detection card to determine the blood type.

[0056] In an embodiment of the present invention, a blood sample, saline and anti-red blood cells are added to a blood processing chip by the method in the embodiment of the present invention, wherein each blood processing chip is loaded into a centrifuge; based on the first centrifugation of the blood, upper plasma and red blood cells are separated, and saline enters the mixing zone; the centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the saline in the mixing zone due to the centrifugal effect; in the fourth centrifugation, the centrifuge is rotated forward and reversed, and the red blood cells are re-mixed by oscillation, and the blood is resuspended, wherein the blood is separated multiple times along the vertical direction; the centrifuge The centrifugal tray is rotated 90 degrees and left to stand for 3 minutes, and the heavy suspension in the mixing area enters the capillary quantitative flow channel; the blood processing chip cooperates with the detection card to determine the blood type, wherein the blood sample, physiological saline and anti-fixed red blood cells are in the blood processing chip, and multiple separations are performed in the blood processing chip to facilitate multiple separations of the blood sample, physiological saline and anti-fixed red blood cells. At the same time, the blood is separated multiple times in the vertical direction, and the blood processing chip cooperates with the detection card to realize the determination of the blood type, thereby improving the coordination effect between the blood processing chip and the detection card. At this time, the blood processing chip is separated in the vertical direction, so that the centrifuge is compatible with multiple blood processing chips for synchronous separation, and the circumferential space of the centrifuge is effectively utilized.

[0057] In step S11, a blood sample, physiological saline and anti-red blood cells are added to a blood processing chip, wherein each blood processing chip is loaded into a centrifuge;

[0058] In an embodiment of the present application, a blood sample is added to the blood feeding area of ​​a blood processing chip, wherein the plasma separation area is located below the blood feeding area; physiological saline is added to the physiological saline feeding area of ​​the blood processing chip, wherein the physiological saline feeding area is located on the right side of the blood feeding area; and reversed red blood cells are added to the reversed red blood cell feeding area of ​​the blood processing chip, wherein the reversed red blood cell feeding area is located below the physiological saline feeding area.

[0059] Among them, based on the fact that the blood sample, saline and anti-fixed red blood cells are contained in the blood processing chip, and the blood sample, saline and anti-fixed red blood cells are in corresponding areas in the blood processing chip, it is convenient for the blood sample, saline and anti-fixed red blood cells to be arranged at intervals for further separation. At this time, the plasma separation area is below the blood feeding area; saline is added to the saline feeding area of ​​the blood processing chip. Optionally, the saline feeding area is on the right side of the blood feeding area, and the anti-fixed red blood cell feeding area is on the lower side of the saline feeding area.

[0060] In step S12, the upper plasma and red blood cells are separated based on the first centrifugation of the blood, and the physiological saline enters the mixing zone;

[0061] In the embodiments of the present application,

[0062] In the specific implementation process of the present invention, the specific steps may be:

[0063] S121: The blood is centrifuged for the first time, and the blood flows vertically downward from the blood feeding area to the plasma separation area and the red blood cell separation area, and the upper plasma and red blood cells are separated; wherein the centrifugation parameters are 2000 rpm and 4 mins;

[0064] S122: physiological saline is separated synchronously and enters the mixing zone in sequence;

[0065] In the embodiment of the present application, the blood is centrifuged for the first time, and the blood flows vertically downward from the blood feeding area to the plasma separation area and the red blood cell separation area. At this time, the blood sample in the blood feeding area is processed, and the blood is centrifuged for the first time. As the first centrifugation of the blood, it is mainly to separate the upper plasma and red blood cells, so as to further process the separated upper plasma and red blood cells, and the separated upper plasma and red blood cells are respectively in the plasma separation area and the red blood cell separation area, so as to achieve further processing of the plasma and red blood cells. Among them, the centrifugation parameters are 2000rpm and 4mins.

[0066] At the same time, the physiological saline is separated synchronously and enters the mixing zone in sequence, so that the physiological saline enters the mixing zone in advance, so that the physiological saline can create a physiological environment.

[0067] In step S13, the centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect;

[0068] In the specific implementation process of the present invention, the specific steps may be:

[0069] S131: After the centrifuge stops working, the red blood cells enter the siphon flow channel in sequence and trigger the siphon valve of the siphon flow channel;

[0070] S132: In the second centrifugation, the centrifugation parameters are 1000 rpm and 2 mins, and the siphon valve is in an open state;

[0071] S133: Most of the plasma enters the plasma quantification area from the plasma separation area, and the excess plasma enters the plasma waste liquid area;

[0072] S134: Most of the red blood cells move from the red blood cell separation area to the red blood cell buffer area and the red blood cell quantification area under the siphon effect of the siphon flow channel, and the excess red blood cells enter the red blood cell waste liquid area.

[0073] In an embodiment of the present application, after the centrifuge stops working, red blood cells enter the siphon flow channel in sequence and trigger the siphon valve of the siphon flow channel so that the siphon flow channel is in an open state, wherein the red blood cells enter the siphon flow channel in sequence and trigger the second centrifugation and the third centrifugation in sequence.

[0074] At this time, after the centrifuge stops working, the red blood cells enter the siphon flow channel in turn and trigger the siphon valve of the siphon flow channel; in the second centrifugation, the centrifugation parameters are 1000rpm and 2mins, and the siphon valve is in an open state to facilitate further separation of plasma and red blood cells. At this time, most of the plasma enters the plasma quantification area from the plasma separation area, and the excess plasma enters the plasma waste liquid area, further realizing the separation of plasma. At the same time, most of the red blood cells move from the red blood cell separation area to the red blood cell buffer area and the red blood cell quantification area under the siphon action of the siphon flow channel, and the excess red blood cells enter the red blood cell waste liquid area, further realizing the separation of red blood cells.

[0075] S135: The centrifuge is suspended, the siphon channel of the red blood cell buffer is filled, and the third separation is triggered;

[0076] S136: in the third centrifugation, the centrifugation parameters are 1000 rpm and 2 mins, and the siphon valve is in an open state;

[0077] S137: Red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect.

[0078] In an embodiment of the present application, the centrifuge is suspended, the siphon flow channel of the red blood cell buffer area is filled, and the third separation is triggered. In the third centrifugation, the centrifugation parameters are 1000rpm and 2mins, the siphon valve is in an open state, and the red blood cells are further separated after the second separation, so that the red blood cells enter the mixing area from the red blood cell quantification area; the red blood cells are stratified with the physiological saline in the mixing area due to the centrifugal effect, thereby completing the separation of the red blood cells, so that the red blood cells and the physiological saline are further mixed.

[0079] S14: The fourth centrifugation, the centrifuge is rotated forward and reversed, the blood is re-mixed by oscillation, and the red blood cells are resuspended, wherein the blood is separated multiple times in the vertical direction;

[0080] In the specific implementation process of the present invention, the specific steps may be:

[0081] S141: In the fourth centrifugation, the centrifuge rotates forward and reversely, and drives the red blood cells and physiological saline in the mixing zone to further mix;

[0082] S142: re-mixing by oscillation to resuspend the red blood cells, wherein the blood is separated multiple times in the vertical direction;

[0083] In an embodiment of the present application, in the fourth centrifugation, the centrifuge rotates forward and reversely, and drives the red blood cells and saline in the mixing zone to further mix. The forward and reverse rotation of the centrifuge is used to adjust the position of the red blood cells and the saline, and the red blood cells and the saline are further mixed so that they can be re-mixed by oscillation and the red blood cells are resuspended, wherein the blood is separated multiple times in the vertical direction.

[0084] In an embodiment of the present application, the blood is separated multiple times in the vertical direction, and the various spaces of the blood processing chip are arranged in the vertical direction so that the blood can flow by its own weight and is further driven by the pump body. At this time, the blood is gradually separated during multiple centrifugations, and in the fourth centrifugation, the centrifuge rotates forward and reversely, and drives the red blood cells and saline in the mixing area to further mix, so that the red blood cells and saline form a heavy suspension in the mixing area.

[0085] S15: The centrifugal tray rotates 90 degrees and stands for 3 minutes, and the resuspended liquid in the mixing area enters the capillary quantitative flow channel;

[0086] In the specific implementation process of the present invention, the specific steps may be:

[0087] S151: The centrifugal tray is rotated 90 degrees and left to stand for 3 minutes;

[0088] S152: The resuspended liquid in the mixing zone enters the capillary quantitative flow channel and is transferred from the front side of the blood processing chip to the back side of the blood processing chip;

[0089] In an embodiment of the present application, the centrifuge tray is rotated 90 degrees and left to stand for 3 minutes to allow the resuspended liquid in the mixing zone to enter the capillary quantitative flow channel and flow through the front and back sides of the blood treatment chip using the capillary quantitative flow channel, thereby utilizing the front and back spaces of the blood treatment chip and transferring from the front side of the blood treatment chip to the back side of the blood treatment chip.

[0090] S16: The blood processing chip cooperates with the detection card to determine the blood type;

[0091] In the specific implementation process of the present invention, the specific steps may be: tearing open the sealed aluminum foil, loading the blood treatment chip into the test card according to the marked direction, and connecting it through the snap-fit ​​of interference fit; releasing the liquid in all quantitative areas, releasing the liquid into the test card, and centrifuging the liquid in the test card into the microcolumn gel; determining the blood type by comparing the agglutination reaction of the test card, wherein the test card is located below the blood treatment chip and is connected to the blood treatment chip;

[0092] In the embodiment of the present application, since there are different areas on the front and back sides of the blood processing chip, and the separation direction of the blood is also downward, the blood processing chip is loaded onto the test card according to the marked direction, and loaded in the marked direction to facilitate installation in the correct direction. At the same time, the liquid in all quantitative areas is released, and the liquid is released into the test card, and the liquid in the test card is centrifuged into the microcolumn gel; the blood type is determined by comparing the agglutination reaction of the test card, wherein the test card is located below the blood processing chip and is connected to the blood processing chip.

[0093] In an embodiment of the present invention, by the method in the embodiment of the present invention, in an embodiment of the present invention, by the method in the embodiment of the present invention, a blood sample, saline and anti-red blood cells are added to a blood processing chip, wherein each blood processing chip is loaded on a centrifuge; based on the first centrifugation of the blood, upper plasma and red blood cells are separated, and saline enters the mixing zone; the centrifuge stops working, and the red blood cells enter the siphon flow channel in turn, and the second centrifugation and the third centrifugation are triggered in turn, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the saline in the mixing zone due to the centrifugal effect; in the fourth centrifugation, the centrifuge is reversed, and the red blood cells are resuspended by oscillation, wherein the blood is vertically rotated. The centrifugal tray is rotated 90 degrees and left to stand for 3 minutes, and the heavy suspension in the mixing area enters the capillary quantitative flow channel; the blood processing chip cooperates with the detection card, and the blood type is determined, wherein the blood sample, physiological saline and anti-fixed red blood cells are in the blood processing chip, and multiple separations are performed in the blood processing chip, so as to facilitate multiple separations of the blood sample, physiological saline and anti-fixed red blood cells. At the same time, the blood is separated multiple times along the vertical direction, and the blood processing chip cooperates with the detection card to realize the determination of the blood type, thereby improving the cooperation effect between the blood processing chip and the detection card. At this time, the blood processing chip is separated in the vertical direction, so that the centrifuge is compatible with multiple blood processing chips for synchronous separation, and the circumferential space of the centrifuge is effectively utilized.

[0094] In addition, the control method of the blood treatment chip provided in the embodiment of the present invention is described in detail above. Specific examples are used herein to illustrate the principle and implementation mode of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of ​​the present invention, there will be changes in the specific implementation mode and application scope. In summary, the content of this specification should not be understood as limiting the present invention.

Claims

1. A control method for a blood processing chip, characterized in that: Applied to blood processing chips; The control method of the blood processing chip comprises: Adding blood samples, physiological saline and anti-red blood cells to blood processing chips, wherein each blood processing chip is loaded into a centrifuge; Based on the first centrifugation of blood, plasma and red blood cells are separated, and saline enters the mixing zone; The centrifuge stops working, and the red blood cells enter the siphon flow channel in turn, triggering the second centrifugation and the third centrifugation in turn. The siphon flow channel is in an open state, and the red blood cells enter the mixing area from the red blood cell quantitative area. The red blood cells are separated from the physiological saline in the mixing area due to the centrifugal effect. In the fourth centrifugation, the centrifuge rotates forward and reversely, remixes by oscillation, and the red blood cells are resuspended. During this process, the blood is separated multiple times in the vertical direction. The centrifugal tray rotates 90 degrees and stands for 3 minutes, and the resuspended liquid in the mixing area enters the capillary quantitative flow channel; The blood processing chip cooperates with the test card to determine the blood type.

2. The control method of the blood treatment chip according to claim 1, characterized in that: The blood sample, physiological saline and anti-red blood cells are added to the blood processing chip, wherein each blood processing chip is loaded into a centrifuge, including: The blood sample is added to the blood feeding area of ​​the blood processing chip, wherein the plasma separation area is located below the blood feeding area; Physiological saline is added to the physiological saline feeding area of ​​the blood processing chip, and the physiological saline feeding area is on the right side of the blood feeding area; The anti-fixed red blood cells are added to the anti-fixed red blood cell feeding area of ​​the blood processing chip, and the anti-fixed red blood cell feeding area is located at the lower side of the physiological saline feeding area.

3. The control method of the blood treatment chip according to claim 2, characterized in that: The first centrifugation of blood to separate plasma and red blood cells, and the physiological saline entering the mixing area, includes: The blood is centrifuged for the first time, and the blood flows vertically downward from the blood feeding area to the plasma separation area and the red blood cell separation area, and the upper plasma and red blood cells are separated; wherein the centrifugation parameters are 2000rpm and 4mins; Physiological saline is separated synchronously and enters the mixing zone in sequence.

4. The control method of the blood treatment chip according to claim 3, characterized in that: The centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, including: After the centrifuge stops working, the red blood cells enter the siphon flow channel one by one and trigger the siphon valve of the siphon flow channel.

5. The control method of the blood processing chip according to claim 4, characterized in that: The centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, and further comprising: In the second centrifugation, the centrifugation parameters were 1000 rpm and 2 mins, and the siphon valve was in the open state; Most of the plasma enters the plasma quantification area from the plasma separation area, and the excess plasma enters the plasma waste liquid area; Most of the red blood cells move from the red blood cell separation area to the red blood cell buffer area and the red blood cell quantification area under the siphon effect of the siphon flow channel, and the excess red blood cells enter the red blood cell waste liquid area.

6. The control method of the blood processing chip according to claim 5, characterized in that: The centrifuge stops working, and the red blood cells enter the siphon flow channel in sequence, and the second centrifugation and the third centrifugation are triggered in sequence, wherein the siphon flow channel is in an open state, and the red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect, and further comprising: The centrifuge is paused, the siphon channel of the red blood cell buffer is filled, and the third separation is triggered; In the third centrifugation, the centrifugation parameters were 1000 rpm and 2 mins, and the siphon valve was in the open state; The red blood cells enter the mixing zone from the red blood cell quantitative zone; the red blood cells are separated from the physiological saline in the mixing zone due to the centrifugal effect.

7. The control method of the blood processing chip according to claim 6, characterized in that: In the fourth centrifugation, the centrifuge rotates forward and reversely, remixes by oscillation, and the red blood cells are resuspended, wherein the blood is separated multiple times in the vertical direction, including: In the fourth centrifugation, the centrifuge rotates forward and reversely, and further mixes the red blood cells and saline in the mixing area; The red blood cells are resuspended by remixing through vortexing, wherein the blood is separated multiple times in the vertical direction.

8. The control method of the blood processing chip according to claim 7, characterized in that: The centrifugal tray is rotated 90 degrees and left to stand for 3 minutes, and the resuspended liquid in the mixing zone enters the capillary quantitative flow channel, including: The centrifuge tray was rotated 90 degrees and allowed to stand for 3 minutes; The resuspended liquid in the mixing area enters the capillary quantitative flow channel and is transferred from the front side of the blood processing chip to the back side of the blood processing chip.

9. The control method of the blood processing chip according to claim 8, characterized in that: The blood processing chip cooperates with the detection card to determine the blood type, including: Tear off the sealed aluminum foil, load the blood processing chip onto the test card according to the marked direction, and connect it through the interference fit buckle.

10. The control method of the blood processing chip according to claim 9, characterized in that: The blood processing chip cooperates with the detection card to determine the blood type, and also includes: Release all the liquid in the quantitative area, release the liquid into the test card, and centrifuge the liquid in the test card into the microcolumn gel; determine the blood type by comparing the agglutination reaction of the test card, wherein, The detection card is located below the blood processing chip and is connected to the blood processing chip.

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