Pre-dilution sample adding method of automatic analytical instrument
By setting specific hole positions and operating positions on the sample loading disc of the automatic analysis device and adopting a specific pre-dilution sample loading mode, the bubble problem caused by the vibration of the mixer is solved, and the accuracy of the analysis and detection results are improved.
Patent Information
- Application Number
- CN202510093979.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-21
- Publication Date
- 2025-04-29
AI Technical Summary
In the pre-dilution and sample filling method of the automatic analysis device, the mixer causes bubbles to exist in the liquid in the reaction cup through a small amplitude vibration, which affects the accuracy of the analysis and detection results.
By setting at least four hole positions on the sample tray and setting the handle, sample needle, reagent needle and mixing operation position in turn on the rotation path of the sample tray, a specific pre-dilution sample tray is adopted, including dividing the time slices and performing specific operating steps to ensure that the liquid in the reaction cup is fully mixed and bubbles are dissipated.
The better mixing effect of the liquid in the reaction cup and the effective dispersion of bubbles are achieved, thereby improving the accuracy of the analysis and detection results of the automatic analysis device.
Smart Images
Figure CN119901932A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of medical devices, and more specifically, relates to a pre-dilution sample addition method of an automatic analysis instrument. Background Art
[0002] In the related art, a type of automatic analysis device, such as a chemiluminescence immunoassay analyzer, usually implements its sample loading process based on a corresponding sample loading mechanism, which includes a sample loading plate with multiple wells and a gripper operation position, a sample needle operation position, a reagent needle operation position, and a mixing operation position sequentially arranged on the rotation path of the sample loading plate. Pre-dilution loading is a commonly used sample loading method for the above-mentioned automatic analysis device, and its specific process is as follows: control the gripper to grab a reaction cup and place it in the target well that is currently in the gripper operation position; rotate the sample loading plate to move the target well to the sample needle operation position; control the sample needle to add sample to the reaction cup in the target well; rotate the sample loading plate to move the target well to the reagent needle operation position; control the reagent needle to add diluent to the reaction cup in the target well; rotate the sample loading plate to move the target well to the mixing operation position; control the mixer to mix the reaction cup in the target well; rotate the sample loading plate to move the target well to the mixing operation position. Rotate to the sample needle operation position; control the sample needle to absorb the diluted sample in the reaction cup in the target well; rotate the sample adding plate to rotate the target well to the gripper operation position; control the gripper to throw the cup and replace the new cup at the target well; in the process of controlling the sample adding plate to rotate accordingly, add the diluted sample to the new reaction cup in the target well through the sample needle, add the reagent to the new reaction cup in the target well through the reagent needle, and mix the new reaction cup in the target well through the mixer; rotate the sample adding plate to move the target well to the gripper operation position, and control the gripper to move the reaction cup to the incubation plate.
[0003] The pre-dilution sample addition method of the above automatic analysis device has the following main problems in practical application:
[0004] Since the mixer achieves the mixing operation by causing the reaction cup to vibrate slightly, bubbles will inevitably exist in the liquid in the reaction cup after the reaction cup containing the diluted sample and the reagent solution is mixed, and the presence of bubbles will affect the accuracy of the final analysis and detection results of the automatic analysis device to a certain extent. Summary of the invention
[0005] The purpose of the present invention is to solve the problem in the related art that the pre-dilution sample addition method of the automatic analysis device affects the accuracy of the analysis and detection results of the automatic analysis device.
[0006] According to a first aspect of the present invention, there is provided a pre-dilution sample addition method of an automatic analysis instrument, the pre-dilution sample addition method being implemented based on a sample addition mechanism, the sample addition mechanism comprising a sample addition plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample addition plate;
[0007] The sample loading plate has at least four wells, and the distribution of the wells on the sample loading plate meets the following conditions: if any well is currently in the mixing operation position, another well is currently in the gripper operation position; if and only if a well is in the gripper operation position, the sample needle operation position, the reagent needle operation position or the mixing operation position, the gripper, the sample needle, the reagent needle and the mixer perform corresponding operations on the reaction cup in the well;
[0008] The pre-dilution sample addition method includes a first pre-dilution sample addition mode, wherein the first pre-dilution sample addition mode divides the cycle time into time slices s1 to s6, and performs the following operations:
[0009] Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position.
[0010] The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position;
[0011] The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X1 hole is rotated to the gripper operation position. During the time slice s5, the gripper performs the operations of throwing the cup and replacing the new cup at the X1 hole. During the time slice s6, the X1 hole is rotated to the sample needle operation position.
[0012] In the N+2th cycle: in the s1th time slice, the sample is diluted with the reaction cup at the X1th well. In the s2th time slice, the X1th well is rotated to the reagent needle operation position. In the s3th time slice, the reagent is added to the reaction cup at the X1th well. In the s4th time slice, the X1th well is rotated to the mixing operation position. In the s5th time slice, the mixer mixes the reaction cup at the X1th well. The gripper moves the reaction cup at the X2th well, which is currently in the gripper operation position, to the incubation plate, grabs a new reaction cup and puts it in the X2th well. In the s6th time slice, the X2th well is rotated to the sample needle operation position. Among them, the reaction cup moved from the X2th well to the incubation plate is the reaction cup of the previous pre-dilution loading project that completed the pre-dilution loading in the N-1th cycle.
[0013] According to a second aspect of the invention, another pre-dilution sample loading method of an automatic analyzer is provided, wherein the pre-dilution sample loading method is implemented based on a sample loading mechanism, wherein the sample loading mechanism comprises a sample loading plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample loading plate;
[0014] The sample loading plate has at least four wells, and the distribution of the wells on the sample loading plate meets the following conditions: if any well is currently in the mixing operation position, another well is currently in the gripper operation position; if and only if a well is in the gripper operation position, the sample needle operation position, the reagent needle operation position or the mixing operation position, the gripper, the sample needle, the reagent needle and the mixer perform corresponding operations on the reaction cup in the well;
[0015] The pre-dilution sample addition method includes a second pre-dilution sample addition mode, wherein the second pre-dilution sample addition mode divides the cycle time into time slices s1 to s6, and performs the following operations:
[0016] Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position.
[0017] The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position;
[0018] The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X2 hole is rotated to the gripper operation position. During the time slice s5, the gripper grabs a new reaction cup and puts it in the X2 hole. During the time slice s6, the X2 hole is rotated to the sample needle operation position.
[0019] Cycle N+2: In the time slice s1, the sample is diluted with the reaction cup of well X2. In the time slice s2, well X2 is rotated to the reagent needle operation position. In the time slice s3, the reagent is added to the reaction cup of well X2. In the time slice s4, well X2 is rotated to the mixing operation position. In the time slice s5, the mixer mixes the reaction cup of well X2. In the time slice s6, well X1 is rotated to the sample needle operation position.
[0020] The N+3th cycle: in the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at well X1; in the time slice s4, well X2 rotates to the gripper operation position; in the time slice s5, the gripper transfers the reaction cup in well X2 to the incubation plate and places a new reaction cup in well X2; in the time slice s6, well X2 rotates to the sample needle operation position.
[0021] The beneficial effects of the present invention are:
[0022] The pre-dilution and sample loading method of the automatic analyzer of the present invention, the first pre-dilution and sample loading mode is applicable to the situation that the prepared diluted sample can only be used for the current sample loading and cannot be used for subsequent sample loading, so the X1 hole position needs to be thrown in the s5 time slice of the N+1th cycle. In the first pre-dilution and sample loading mode, the reaction cup containing the diluted sample and the reagent solution will not be transferred to the incubation disk in the current cycle after the mixing operation, but will be transferred to the incubation link after three more cycles. Specifically, in the s5 time slice of the N+2th cycle, the gripper moves the reaction cup in the X2 hole position currently in the gripper operation position to the incubation disk. The reaction cup moved from the X2 hole position to the incubation disk is the reaction cup of the last pre-dilution and sample loading project that completed the pre-dilution and sample loading in the N-1th cycle. It can be seen that the reaction cup that completed the pre-dilution and sample loading in the N-1th cycle is transferred to the incubation disk by the gripper in the N+2th cycle. During the above three cycles, the reaction cup continues to rotate with the sample adding plate. The continued rotation process includes multiple start and stop processes. After each start and stop process, the liquid in the reaction cup will shake more significantly than the mixing operation due to inertia. This liquid shaking phenomenon will promote further mixing of the liquid in the reaction cup on the one hand, and is beneficial to the dispersion of bubbles in the liquid on the other hand.
[0023] The pre-dilution sample addition method of the automatic analyzer of the present invention, the second pre-dilution sample addition mode is applicable to the situation that the diluted sample prepared this time can be used for subsequent sample addition, and there is no need to throw the cup in the N+1th cycle, because the diluted sample in the reaction cup of the X1 hole can continue to be used. The reaction cup that has undergone the mixing operation in the s5 time slice in the N+2th cycle will not flow to the incubation link in the s6 time slice of the cycle, but will flow to the incubation link in the s5 time slice in the N+3th cycle. It can be seen that compared with the way in which the s6 time slice in the N+2th cycle flows to the incubation link, the reaction cup has undergone one more start-stop process, the mixing effect of the liquid in the reaction cup will be relatively better, and the amount of bubbles contained will be relatively less.
[0024] Based on the above, it can be seen that the pre-dilution and sample addition method of the automatic analyzer of the present invention can obtain an incubation solution with relatively better mixing effect and relatively less bubbles, thereby improving the accuracy of the analysis and detection results of the automatic analyzer to a certain extent.
[0025] Other features and advantages of the present invention will be described in detail in the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] The present invention may be better understood by referring to the following description taken in conjunction with the accompanying drawings, in which the same or similar reference numerals are used throughout the drawings to represent the same or similar components.
[0027] Figure 1 A flowchart for implementing the first pre-dilution sample addition mode according to an embodiment of the present invention is shown;
[0028] Figure 2 A schematic structural diagram of a sample loading mechanism according to an embodiment of the present invention is shown. DETAILED DESCRIPTION
[0029] In order to enable those skilled in the art to more fully understand the technical solution of the present invention, the exemplary embodiments of the present invention will be described in more comprehensive and detailed in conjunction with the accompanying drawings below. Obviously, the one or more embodiments of the present invention described below are only one or more of the specific ways in which the technical solution of the present invention can be implemented, and are not exhaustive. It should be understood that other ways belonging to a general inventive concept can be used to implement the technical solution of the present invention, and should not be limited by the exemplary embodiments described. Based on one or more embodiments of the present invention, all other embodiments obtained by ordinary technicians in the field without making creative work should fall within the scope of protection of the present invention.
[0030] Example: Figure 1 FIG. 1 shows a flow chart of the implementation of the first pre-dilution sample addition mode of an embodiment of the present invention. Figure 1 The pre-dilution sample addition method of the embodiment of the present invention is implemented based on a sample addition mechanism, which includes a sample addition plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on the rotation path of the sample addition plate;
[0031] The sample loading plate has at least four wells, and the distribution of the wells on the sample loading plate meets the following conditions: if any well is currently in the mixing operation position, there is another well that is currently in the gripper operation position; if and only if a well is in the gripper operation position, the sample needle operation position, the reagent needle operation position or the mixing operation position, the gripper, the sample needle, the reagent needle and the mixer perform corresponding operations on the reaction cup in the well;
[0032] The pre-dilution sample addition method includes a first pre-dilution sample addition mode, which divides the cycle time into time slices s1 to s6 and performs the following operations:
[0033] Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position.
[0034] The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position;
[0035] The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X1 hole is rotated to the gripper operation position. During the time slice s5, the gripper performs the operations of throwing the cup and replacing the new cup at the X1 hole. During the time slice s6, the X1 hole is rotated to the sample needle operation position.
[0036] In the N+2th cycle: in the s1th time slice, the sample is diluted with the reaction cup at the X1th well. In the s2th time slice, the X1th well is rotated to the reagent needle operation position. In the s3th time slice, the reagent is added to the reaction cup at the X1th well. In the s4th time slice, the X1th well is rotated to the mixing operation position. In the s5th time slice, the mixer mixes the reaction cup at the X1th well. The gripper moves the reaction cup at the X2th well, which is currently in the gripper operation position, to the incubation plate, grabs a new reaction cup and puts it in the X2th well. In the s6th time slice, the X2th well is rotated to the sample needle operation position. Among them, the reaction cup moved from the X2th well to the incubation plate is the reaction cup of the previous pre-dilution loading project that completed the pre-dilution loading in the N-1th cycle.
[0037] Specifically, Figure 2 FIG. 2 shows a schematic diagram of the structure of the sample loading mechanism according to an embodiment of the present invention. Figure 2 In the embodiment of the present invention, the sample adding plate 100 has 10 holes 110, which are hole positions 1 to 10 respectively; the gripper operation position 200, the sample needle operation position 300, the reagent needle operation position 400 and the mixing operation position 500 are sequentially arranged on the rotation path of the sample adding plate 100, wherein hole position 1 is currently in the gripper operation position 200, hole position 3 is currently in the sample needle operation position 300, hole position 7 is currently in the reagent needle operation position 400, and hole position 9 is currently in the mixing operation position 500. A mixer is arranged under the mixing operation position 500. When the cuvette in the hole position currently in the mixing operation position 500 needs to be mixed, the mixer is first raised to make it contact with the cuvette, and then the mixer is turned on.
[0038] Furthermore, the pre-dilution sample addition method of the embodiment of the present invention further includes a second pre-dilution sample addition mode, wherein the second pre-dilution sample addition mode divides the cycle time into time slices s1 to s6, and performs the following operations:
[0039] Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position.
[0040] The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position;
[0041] The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X2 hole is rotated to the gripper operation position. During the time slice s5, the gripper grabs a new reaction cup and puts it in the X2 hole. During the time slice s6, the X2 hole is rotated to the sample needle operation position.
[0042] Cycle N+2: In the time slice s1, the sample is diluted with the reaction cup of well X2. In the time slice s2, well X2 is rotated to the reagent needle operation position. In the time slice s3, the reagent is added to the reaction cup of well X2. In the time slice s4, well X2 is rotated to the mixing operation position. In the time slice s5, the mixer mixes the reaction cup of well X2. In the time slice s6, well X1 is rotated to the sample needle operation position.
[0043] The N+3th cycle: in the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at well X1; in the time slice s4, well X2 rotates to the gripper operation position; in the time slice s5, the gripper transfers the reaction cup in well X2 to the incubation plate and places a new reaction cup in well X2; in the time slice s6, well X2 rotates to the sample needle operation position.
[0044] Specifically, in an embodiment of the present invention, the first pre-dilution loading mode is applicable to the situation that the prepared diluted sample can only be used for the current loading and cannot be used for subsequent loading. Therefore, the cup needs to be thrown for hole position X1 in the s5 time slice of the N+1th cycle; in the s5 time slice of the N+2th cycle, the gripper moves the reaction cup in hole position X2 currently in the gripper operation position to the incubation tray. The reaction cup moved from hole position X2 to the incubation tray is the reaction cup of the last pre-dilution loading project that completed the pre-dilution loading in the N-1th cycle. It can be seen that the reaction cup that completed the pre-dilution loading in the N-1th cycle is transferred to the incubation tray by the gripper in the N+2th cycle. During this interval, the reaction cup continues to rotate with the loading tray. The process of continued rotation includes multiple start-stop processes. After each start-stop process is completed, the liquid in the target reaction cup will shake more significantly due to inertia than the mixing operation. This phenomenon of liquid shaking will promote further mixing of the liquid in the reaction cup on the one hand, and on the other hand, it is conducive to the dispersion of bubbles in the liquid.
[0045] The second pre-dilution loading mode is applicable to the situation where the diluted sample prepared this time can be used for subsequent loading. There is no need to throw the cup in the N+1th cycle, because the diluted sample in the reaction cup at the X1 hole can continue to be used. The reaction cup that has been mixed in the s5 time slice in the N+2th cycle will not flow to the incubation stage in the s6 time slice of the cycle, but will flow to the incubation stage in the s5 time slice in the N+3th cycle. It can be seen that compared with the way that the s6 time slice in the N+2th cycle flows to the incubation stage, the reaction cup has experienced one more start-stop process, the mixing effect of the liquid in the reaction cup will be relatively better, and the amount of bubbles will be relatively less.
[0046] Although one or more embodiments of the present invention are described above, it should be known to those skilled in the art that the present invention can be implemented in any other form without departing from its subject matter and scope. Therefore, the embodiments described above are illustrative rather than restrictive, and many modifications and substitutions are obvious to those skilled in the art without departing from the spirit and scope of the present invention as defined in the appended claims.
Claims
1. A pre-dilution sample addition method for an automatic analysis instrument, characterized in that: The pre-dilution sample addition method is implemented based on a sample addition mechanism, which includes a sample addition plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample addition plate; The sample loading plate has at least four wells, and the distribution of the wells on the sample loading plate meets the following conditions: if any well is currently in the mixing operation position, another well is currently in the gripper operation position; if and only if a well is in the gripper operation position, the sample needle operation position, the reagent needle operation position or the mixing operation position, the gripper, the sample needle, the reagent needle and the mixer perform corresponding operations on the reaction cup in the well; The pre-dilution sample addition method includes a first pre-dilution sample addition mode, wherein the first pre-dilution sample addition mode divides the cycle time into time slices s1 to s6, and performs the following operations: Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position. The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position; The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X1 hole is rotated to the gripper operation position. During the time slice s5, the gripper performs the operations of throwing the cup and replacing the new cup at the X1 hole. During the time slice s6, the X1 hole is rotated to the sample needle operation position. In the N+2th cycle: in the s1th time slice, the sample is diluted with the reaction cup at the X1th well. In the s2th time slice, the X1th well is rotated to the reagent needle operation position. In the s3th time slice, the reagent is added to the reaction cup at the X1th well. In the s4th time slice, the X1th well is rotated to the mixing operation position. In the s5th time slice, the mixer mixes the reaction cup at the X1th well. The gripper moves the reaction cup at the X2th well, which is currently in the gripper operation position, to the incubation plate, grabs a new reaction cup and puts it in the X2th well. In the s6th time slice, the X2th well is rotated to the sample needle operation position. Among them, the reaction cup moved from the X2th well to the incubation plate is the reaction cup of the previous pre-dilution loading project that completed the pre-dilution loading in the N-1th cycle.
2. A pre-dilution sample addition method for an automatic analysis instrument, characterized in that: The pre-dilution sample addition method is implemented based on a sample addition mechanism, which includes a sample addition plate and a gripper operation position, a sample needle operation position, a reagent needle operation position and a mixing operation position sequentially arranged on a rotation path of the sample addition plate; The sample loading plate has at least four wells, and the distribution of the wells on the sample loading plate meets the following conditions: if any well is currently in the mixing operation position, another well is currently in the gripper operation position; if and only if a well is in the gripper operation position, the sample needle operation position, the reagent needle operation position or the mixing operation position, the gripper, the sample needle, the reagent needle and the mixer perform corresponding operations on the reaction cup in the well; The pre-dilution sample addition method includes a second pre-dilution sample addition mode, wherein the second pre-dilution sample addition mode divides the cycle time into time slices s1 to s6, and performs the following operations: Cycle N-1: In the s5 time slice, the gripper grabs a new reaction cup and places it in the X1 hole. In the s6 time slice, the X1 hole is rotated to the sample needle operation position. The Nth cycle: in the s1 time slice, the sample is added to the reaction cup of the X1 well, in the s2 time slice, the X1 well is rotated to the reagent needle operation position, in the s3 time slice, the reagent is added to the reaction cup of the X1 well, in the s4 time slice, the X1 well is rotated to the mixing operation position, in the s5 time slice, the mixer performs a mixing operation on the reaction cup of the X1 well, and in the s6 time slice, the X1 well is rotated to the sample needle operation position; The N+1th cycle: During the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at the X1 hole. During the time slice s4, the X2 hole is rotated to the gripper operation position. During the time slice s5, the gripper grabs a new reaction cup and puts it in the X2 hole. During the time slice s6, the X2 hole is rotated to the sample needle operation position. Cycle N+2: In the time slice s1, the sample is diluted with the reaction cup of well X2. In the time slice s2, well X2 is rotated to the reagent needle operation position. In the time slice s3, the reagent is added to the reaction cup of well X2. In the time slice s4, well X2 is rotated to the mixing operation position. In the time slice s5, the mixer mixes the reaction cup of well X2. In the time slice s6, well X1 is rotated to the sample needle operation position. The N+3th cycle: in the time slices s1 to s3, the sample needle absorbs the diluted sample in the reaction cup at well X1; in the time slice s4, well X2 rotates to the gripper operation position; in the time slice s5, the gripper transfers the reaction cup in well X2 to the incubation plate and places a new reaction cup in well X2; in the time slice s6, well X2 rotates to the sample needle operation position.