Hydro-gloss needle essence and preparation method thereof

Abstract

The invention relates to the field of beauty product processing, and particularly discloses a hydro-gloss needle essence and a preparation method thereof. The invention relates to a water-light needle essence, which comprises an essence component and a solvent component, the solvent comprises the following components in percentage by weight: 0.05%-5% of acetylchitosamine, 0.05%-0.3% of allantoin, 0.11%-3% of nicotinamide, 0.11%-5% of panthenol, 0.11%-5% of carnosine, 0.11%-7% of Ectoin, 0.5%-1% of buffer salt, 0.11%-3% of vitamin, 0.05%-3% of amino acid and the balance of water; the preparation method comprises the following steps: preparing the essence components; uniformly mixing the solvent components to obtain the solvent components; adding the solvent component into the essence component, and uniformly mixing to obtain essence; the moisturizing cream has the advantages that the moisturizing time is long, collagen regeneration is stimulated, the skin elasticity and tenderness are improved, the skin is tightened, and allergy is not prone to occurring.

Description

Technical Field

[0001] The present application relates to the field of beauty product processing, and more specifically, to a water light needle essence and a preparation method thereof. Background Art

[0002] Human skin aging is a gradual physiological process that directly affects the appearance and function of the skin. After skin aging, it is prone to problems such as dull skin tone, lack of luster, accelerated wrinkle growth, etc. As the human body's physiological functions continue to deteriorate, skin collagen is gradually lost and elastic fibers are damaged, causing the skin to become loose and collapsed.

[0003] The working principle of hyaluronic acid injection is to use vacuum negative pressure technology to inject "nutrients" into the dermis of the skin through a tiny needle, so that the functional ingredients can directly act on the mesoderm cells, better exert the anti-aging and beauty functions of the effective ingredients, and play a role in protecting the skin, promoting collagen regeneration, improving intermediate metabolism, and promoting skin nutrient absorption.

[0004] Most of the existing hyaluronic acid hydrating essences are hyaluronic acid hydrating essences, in which physiological saline is often used as a solvent. Physiological saline is only a single solvent and has no nutritional effect or other functions. At the same time, the hyaluronic acid hydrating essence prepared with physiological saline has only moisturizing effect, and does not have other functions such as anti-aging, and the moisturizing time is also short.

[0005] Therefore, how to prepare a new hydrating essence that has the advantages of long-term moisturizing, stimulating collagen regeneration, improving skin elasticity and tenderness, tightening and delicate skin, and not prone to allergic problems is a problem to be solved. Summary of the invention

[0006] In order to prepare a new hydrating needle essence which has the advantages of long-term moisturizing, stimulating collagen regeneration, improving skin elasticity and tenderness, tightening skin, and being less prone to allergic problems, the present application provides a hydrating needle essence and a preparation method thereof.

[0007] In the first aspect, the present application provides a water light needle essence, which adopts the following technical solution: A water light needle essence, comprising an essence component and a solvent component in a mass ratio of 0.5-10:100; The essence component is selected from any one of essence freeze-dried powder, essence freeze-dried sponge or essence solid dressing; The solvent component comprises the following raw materials in weight percentage: 0.05%-5% acetyl glucosamine, 0.05%-0.3% allantoin, 0.11%-3% niacinamide, 0.11%-5% panthenol, 0.11%-5% carnosine, 0.11%-7% ectoine, 0.5%-1% buffer salt, 0.11%-3% vitamins, 0.05%-3% amino acids, and the balance is water.

[0008] By adopting the above technical scheme, the essence components are dissolved by the solvent components to prepare the water light needle essence. As a small molecule, acetyl glucosamine is easily absorbed by the skin and has good anti-inflammatory, repairing and whitening effects; niacinamide can reduce the damage of free radicals to the skin, help prevent skin aging, increase skin gloss, promote energy metabolism, and enhance immunity; panthenol has a good soothing effect, reduces the levels of prostaglandin E2 (PGE2), interleukin-6 (IL-6) and thymic stromal lymphopoietin, reduces inflammatory response, improves sensitive symptoms, calms and soothes the skin; panthenol can also protect against oxidative stress damage, upregulate the skin's own antioxidant mechanism, promote wound healing, repair damaged skin, niacinamide cooperates with panthenol, and cooperates with acetyl glucosamine and other substances to reach the skin mesoderm to ensure the uniform distribution of the essence. Ecdoin is an important substance that ensures that highly halophilic bacteria do not die of dehydration in a high-salt environment. It can also maintain osmotic pressure balance. Its unique molecular structure gives it a strong ability to complex water molecules. One molecule of Ecdoin can complex four or five water molecules, which can structure the free water in the cell. At the same time, Ecdoin can significantly reduce the light damage of ultraviolet and visible light to DNA in cells, inhibit tyrosinase activity, reduce melanin formation, and has a potential whitening effect. Carnosine has a strong antioxidant property, which can remove active oxygen free radicals in the process of oxidative stress, promote the production of dermal proteins, maintain the firmness and elasticity of the skin, prevent damage induced by visible light, and inhibit the increase of melanin induced by blue light.

[0009] By utilizing the water molecule complexing ability of Ecdoin, a thicker hydration layer is formed around the biological macromolecules in the essence components, thereby prolonging the moisturizing effect of the essence.

[0010] Allantoin, carnosine and niacinamide are combined. Niacinamide is an important synthetic raw material in energy metabolism. It participates in the redox reaction in the body, provides energy for cell metabolism, promotes collagen regeneration, and increases skin elasticity. Combined with the softening effect of allantoin on keratin, it improves skin softness and elasticity. Combined with β-alanine and L-histidine in carnosine, it further promotes the synthesis of collagen. Combined with the essence components, it increases skin elasticity and tenderness.

[0011] Preferably, the buffer salt is one or more of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium acetate, ferrous sulfate, zinc sulfate, magnesium sulfate, disodium hydrogen phosphate, and sodium dihydrogen phosphate.

[0012] By adopting the above technical solution, buffer salts can help regulate the skin to a suitable osmotic pressure, maintain the appropriate pH of the product, reduce the irritation of the hydrating essence to the skin, and the buffer salts can stabilize the formula ingredients, making it less likely that the ingredients will decompose before use; at the same time, the ions in the buffer salts can help effective active ingredients penetrate into the skin, protect the skin barrier, and prevent the invasion of harmful substances, thereby improving the moisturizing and repairing effects of the hydrating essence on the skin.

[0013] Preferably, the vitamin is selected from one or more of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B8, vitamin B9, vitamin B12, vitamin A, vitamin C, and vitamin E.

[0014] By adopting the above technical solutions, vitamin B2 can participate in cell metabolism, thereby shrinking pores and tightening the skin; vitamin B6 can promote the body's absorption of minerals and trace elements, and increase the skin's elasticity, toughness and luster; vitamin B8 can accelerate the decomposition of fat, prevent abnormal or excessive accumulation of fat, increase the utilization rate of fat in the body, and also help the metabolism of fatty acids and carbohydrates.

[0015] Preferably, the amino acids are selected from one or more of alanine, glycine, proline, valine, serine, lysine, tyrosine, threonine, tryptophan, phenylalanine, ornithine, arginine, asparagine, glutamic acid, histidine, isoleucine, leucine, aspartic acid, cystine, glutamine, hydroxyproline, and cysteine.

[0016] By adopting the above technical solutions, alanine has a good antioxidant effect, and can also promote cell metabolism and collagen synthesis; glycine can moisturize the skin, enhance the conditioning effect of the skin, balance the oil secretion of the skin, and improve the anti-allergic ability of sensitive skin; proline can promote the production of collagen, promote the skin's own moisturizing ability, slow down the skin's glycosylation reaction, effectively protect collagen and elastic fibers, and maintain the skin's elasticity and firmness; valine can strengthen the skin barrier, supplement skin nutrition, and keep the skin moisturized; serine can inhibit lipid peroxidation, reduce the damage of free radicals to the skin, and help maintain the elasticity and luster of the skin. In addition, serine can inhibit the production of melanin, which can improve skin dullness and spots to a certain extent, and can also promote skin cell regeneration and moisturize the skin.

[0017] Preferably, the acetyl glucosamine is a carrier acetyl glucosamine, and the carrier is made of vitamin C nanoliposomes, sodium alginate solution and acetyl glucosamine in a mass ratio of 1:0.2-0.5:1-2.

[0018] By adopting the above technical solution and utilizing the better penetration effect of nanoliposomes, vitamin C can be easily carried and gradually penetrated into the deep layers of the skin. As a necessary component for collagen synthesis, vitamin C is slowly released to promote the gradual synthesis of collagen in the deep layers of the skin, thereby increasing the collagen content in the deep layers of the skin. In combination with the essence components, the skin's softness, elasticity and firmness can be improved.

[0019] The acetyl glucosamine bonded to the surface of the nanoliposome is evenly dispersed after injection. The acetyl glucosamine can promote cell growth and proliferation, which is convenient for repairing the skin tissue at the injection site and quickly strengthening the skin barrier. Some of the acetyl glucosamine bonded to the surface of the nanoliposome can follow the nanoliposome to move deep into the skin, and with vitamin C, it can further stimulate and promote the synthesis of collagen, increase the elasticity and firmness of the skin. At the same time, as a precursor of hyaluronic acid, acetyl glucosamine can promote the metabolism of skin cells, thereby improving the smoothness and tenderness of the skin surface and reducing dullness and pigmentation.

[0020] Preferably, the essence freeze-dried powder is selected from one or more of sodium hyaluronate freeze-dried powder, collagen freeze-dried powder, carboxyalkyl chitosan freeze-dried powder, carboxyalkyl chitin freeze-dried powder, hydroxyalkyl chitosan freeze-dried powder, hydroxyalkyl chitin freeze-dried powder and succinyl chitosan freeze-dried powder.

[0021] By adopting the above technical solution, the freeze-dried sodium hyaluronate powder has a good water-retaining effect, and can improve skin dryness, increase skin elasticity, increase skin water content, and make the skin plump and radiant.

[0022] Collagen freeze-dried powder can maintain the elasticity and luster of the skin, and can also increase the collagen content, thereby reducing skin wrinkles, increasing skin firmness and elasticity, and making the skin delicate and smooth.

[0023] Chitosan freeze-dried powder and chitosan derivative freeze-dried powder have good biocompatibility and efficacy, such as the advantages of repairing, soothing, moisturizing, anti-wrinkle, anti-inflammatory, and improving skin roughness; carboxymethyl chitosan can promote the growth of normal fibroblasts and epithelial cells and accelerate wound healing; activate related cells in wound healing, such as macrophages and leukocytes, to promptly play a role in promoting wound healing; carboxymethyl chitosan can fully inhibit the production of active enzymes that produce inflammatory factors and the inflammatory factor that causes pain - bradykinin and 5'-hydroxytryptamine, thereby playing an anti-inflammatory and analgesic effect, reducing vasodilation, reducing blood exudation in blood vessels, and alleviating tissue edema, pain, and inflammation.

[0024] Preferably, the essence freeze-dried sponge is prepared from sodium hyaluronate, collagen, modified hydroxyapatite nanowires and carboxymethyl chitosan solution in a mass ratio of 1:0.5-1:0.05-0.1:0.5-1.

[0025] By adopting the above technical solution, after sodium hyaluronate and collagen are mixed, carboxymethyl chitosan solution is used as a binder and modified hydroxyapatite nanowires are used as a filling support to bond sodium hyaluronate, collagen and modified hydroxyapatite nanowires to each other.

[0026] When the essence freeze-dried sponge comes into contact with the solvent component, the modified hydroxyapatite nanowires have a filling effect. After contacting water, the modified hydroxyapatite nanowires become hydrophilic but insoluble in water, which facilitates the rapid dispersion of the solvent in the essence freeze-dried sponge, promotes the dispersion of carboxymethyl chitosan, hyaluronic acid and collagen, and accelerates the dissolution of the freeze-dried sponge. Compared with freeze-dried powder, the freeze-dried sponge has a loose and porous structure, dissolves faster, and the amount of substance can be controlled more accurately.

[0027] Modified hydroxyapatite nanowires have good biocompatibility. Their nanowire shape facilitates the formation of an interwoven three-dimensional structure, which is beneficial to the adhesion and extension of cells and the stable contact between cells. Their surface hydroxyl groups facilitate the connection with sodium hyaluronate, collagen, carboxymethyl chitosan, etc., forming an interwoven network in the skin, further increasing the contact area and contact rate of cells, regulating cell proliferation and differentiation while also being able to repair damaged skin cells. Modified hydroxyapatite nanowires are used as connecting supports, combined with the moisturizing and repairing effects of sodium hyaluronate and carboxymethyl chitosan and collagen, to increase skin elasticity and tenderness, prolong the moisturizing effect time of the hydrating essence, promote skin repair, and increase skin elasticity and tenderness.

[0028] Preferably, the modified hydroxyapatite nanowires are prepared by adsorbing isopropyl myristate onto hydroxyapatite nanowires and then bonding them with carboxymethyl chitosan solution and hyaluronic acid synthase; the mass ratio of hydroxyapatite nanowires, carboxymethyl chitosan solution and hyaluronic acid synthase is 1:0.1-0.2:0.05-0.1.

[0029] By adopting the above technical scheme, the adsorption effect of hydroxyapatite nanowires is utilized, and the lower viscosity of isopropyl myristate is combined to facilitate the adsorption of isopropyl myristate by hydroxyapatite nanowires, so that the surface viscosity of hydroxyapatite nanowires is higher than that of isopropyl myristate, and the better penetration effect of isopropyl myristate is utilized to facilitate the contact of modified hydroxyapatite nanowires with deep skin layers, and the hyaluronic acid synthase on the surface of hydroxyapatite nanowires is utilized to promote the synthesis of hyaluronic acid in deep skin layers, thereby improving the repair effect of deep skin layers, reducing the formation of wrinkles, and ensuring the elasticity and firmness of the skin; and carboxymethyl chitosan can also play a repairing effect on the surface of the skin and promote skin barrier repair.

[0030] In the second aspect, the present application provides a method for preparing a water light needle essence, using the following technical solution: A method for preparing a water light needle essence comprises the following steps: S1, preparing essence components; S2, weighing acetyl glucosamine, allantoin, niacinamide, panthenol, carnosine and water, mixing and stirring evenly, then adding buffer salts, vitamins, and amino acids, continuing to mix and stir evenly, and finally adding ectoin, mixing and stirring evenly, to obtain a solvent component; S3. Add the solvent component to the essence component and mix well. The pH should be 6.0-8.0 to obtain the hydrating essence.

[0031] By adopting the above technical solution, the prepared water light needle essence has the advantages of long-lasting moisturizing, gentle soothing, stimulating collagen regeneration, helping to repair the skin barrier, improving skin elasticity and tenderness, tightening the skin, and not prone to allergies.

[0032] In summary, this application has the following beneficial effects: 1. The water light needle essence is prepared by mixing essence components and solvent components, which has the advantages of long-term moisturizing, stimulating collagen regeneration, improving skin elasticity and tenderness, tightening skin, and not prone to allergies.

[0033] 2. Acetyl glucosamine, allantoin and ectoine are combined to reduce the evaporation of water from the skin surface and ensure the skin is hydrated. Acetyl glucosamine has whitening and antioxidant effects. It is a small molecule monosaccharide that is easily absorbed by the skin and has whitening and antioxidant effects. In addition, ectoine can penetrate into the epidermis and further block the moisture of epidermal cells to ensure that the essence components stay on the skin surface for a long time, moisturizing and repairing the skin, preventing aging and improving wrinkles. Ectoine also has a certain effect of relieving skin itching, reducing inflammation and preventing photoaging, and can relieve eczema and neurodermatitis.

[0034] 3. Nanoliposomes have a better penetration effect, which makes it easy to carry vitamin C and gradually penetrate into the deep layers of the skin. Vitamin C is an essential component for collagen synthesis. With the slow release of vitamin C, it promotes the gradual synthesis of collagen in the deep layers of the skin, thereby increasing the collagen content in the deep layers of the skin. Combined with the essence components, it improves the skin's softness, elasticity and firmness. DETAILED DESCRIPTION

[0035] The present application is further described in detail below in conjunction with embodiments.

[0036] The following raw materials are all commercially available. Preparation Example of Acetyl Chitosan

[0037] The phosphatidylcholine in the following raw materials was purchased from Jiangsu Caiwei Biotechnology Co., Ltd. and is food grade; other raw materials are all commercially available.

[0038] Preparation Example 1: The loaded acetyl glucosamine was prepared by the following method: Weigh 1 g of phosphatidylcholine and 30 mL of ethanol, stir until the phosphatidylcholine is completely dissolved, and then wait for the solvent to completely evaporate to obtain a dry film; add 300 mL of phosphate buffer solution, which is a sodium dihydrogen phosphate aqueous solution with a pH of 7, and perform ultrasonic treatment at 20 kHz for 1 hour to obtain an emulsion; Weigh 0.6 g of vitamin C in 300 mL of phosphate buffer solution, stir evenly, add to the emulsion, ultrasonically vibrate at 20 kHz for 30 min, and centrifuge to obtain vitamin C nanoliposomes; 0.5 kg of sodium alginate solution was evenly sprayed on the surface of 1 kg of vitamin C nanoliposomes, and then 1.5 kg of acetyl glucosamine was added and mixed evenly. The average particle size of the vitamin C nanoliposomes was 100 nm, the average particle size of the acetyl glucosamine was 40 nm, and the sodium alginate solution was a sodium alginate aqueous solution with a mass fraction of 1%. After drying and dispersion, the loaded acetyl glucosamine was obtained.

[0039] Preparation Example 2: The difference between this preparation example and preparation example 1 is: 0.2 kg of sodium alginate solution was evenly sprayed on the surface of 1 kg of vitamin C nanoliposomes, and then 1 kg of acetyl glucosamine was added and mixed and stirred evenly. The average particle size of the vitamin C nanoliposomes was 100 nm, the average particle size of the acetyl glucosamine was 40 nm, and the sodium alginate solution was a sodium alginate aqueous solution with a mass fraction of 1%. After drying and dispersion, the loaded acetyl glucosamine was obtained.

[0040] Preparation Example 3: The difference between this preparation example and preparation example 1 is: 0.5 kg of sodium alginate solution was evenly sprayed on the surface of 1 kg of vitamin C nanoliposomes, and then 2 kg of acetyl glucosamine was added and mixed evenly. The average particle size of the vitamin C nanoliposomes was 100 nm, the average particle size of the acetyl glucosamine was 40 nm, and the sodium alginate solution was a sodium alginate aqueous solution with a mass fraction of 1%. After drying and dispersion, the loaded acetyl glucosamine was obtained. Preparation Example of Essence Freeze-Dried Sponge

[0041] The following raw materials are all commercially available.

[0042] Preparation Example 4: The essence freeze-dried sponge was prepared by the following method: 1 kg of hydroxyapatite nanowires are immersed in 5 kg of isopropyl myristate, the average length of the hydroxyapatite nanowires is 100 nm, and the mixture is stirred and dispersed at a speed of 1000 r / min for 30 min, and the hydroxyapatite nanowires are separated by filtration; then 0.15 kg of carboxymethyl chitosan solution is evenly sprayed on the surface thereof, the carboxymethyl chitosan solution is a carboxymethyl chitosan aqueous solution with a mass fraction of 1%, and finally 0.08 kg of hyaluronic acid synthase is evenly sprayed, the average particle size of the hyaluronic acid synthase is 40 nm, and the mixture is dried and dispersed until the hydroxyapatite nanowires do not stick to each other and agglomerate, thereby obtaining modified hydroxyapatite nanowires; 1 kg of sodium hyaluronate, 0.8 kg of collagen, 0.08 kg of modified hydroxyapatite nanowires and 0.8 kg of carboxymethyl chitosan solution were mixed and stirred evenly, and then freeze-dried in a mold to obtain an essence freeze-dried sponge.

[0043] Preparation Example 5: This preparation example differs from Preparation Example 4 in that: 1 kg of hydroxyapatite nanowires are immersed in 5 kg of isopropyl myristate, the average length of the hydroxyapatite nanowires is 100 nm, and the mixture is stirred and dispersed at a speed of 1000 r / min for 30 min, and the hydroxyapatite nanowires are separated by filtration; then 0.1 kg of carboxymethyl chitosan solution is evenly sprayed on the surface thereof, the carboxymethyl chitosan solution is a carboxymethyl chitosan aqueous solution with a mass fraction of 1%, and finally 0.05 kg of hyaluronic acid synthase is evenly sprayed, the average particle size of the hyaluronic acid synthase is 40 nm, and the mixture is dried and dispersed until the hydroxyapatite nanowires do not stick to each other and agglomerate, thereby obtaining modified hydroxyapatite nanowires; 1 kg of sodium hyaluronate, 0.5 kg of collagen, 0.05 kg of modified hydroxyapatite nanowires and 0.5 kg of carboxymethyl chitosan solution were mixed and stirred evenly, and then freeze-dried in a mold to obtain an essence freeze-dried sponge.

[0044] Preparation Example 6: This preparation example differs from Preparation Example 4 in that: 1 kg of hydroxyapatite nanowires are immersed in 5 kg of isopropyl myristate, the average length of the hydroxyapatite nanowires is 100 nm, and the mixture is stirred and dispersed at a speed of 1000 r / min for 30 min, and the hydroxyapatite nanowires are separated by filtration; then 0.2 kg of carboxymethyl chitosan solution is evenly sprayed on the surface thereof, the carboxymethyl chitosan solution is a carboxymethyl chitosan aqueous solution with a mass fraction of 1%, and finally 0.1 kg of hyaluronic acid synthase is evenly sprayed, the average particle size of the hyaluronic acid synthase is 40 nm, and the mixture is dried and dispersed until the hydroxyapatite nanowires do not stick to each other and agglomerate, thereby obtaining modified hydroxyapatite nanowires; 1 kg of sodium hyaluronate, 1 kg of collagen, 0.1 kg of modified hydroxyapatite nanowires and 1 kg of carboxymethyl chitosan solution were mixed and stirred evenly, and then freeze-dried in a mold to obtain an essence freeze-dried sponge. Example

[0045] The following raw materials are all commercially available.

[0046] Example 1: A water light needle essence: The mass ratio of the essence component to the solvent component is 1.5:100; The essence component is essence freeze-dried powder, and the essence freeze-dried powder is carboxymethyl chitosan freeze-dried powder; Solvent components: 0.05% acetyl glucosamine, 0.05% allantoin, 0.11% niacinamide, 0.11% panthenol, 0.11% carnosine, 0.11% ectoine, 0.5% buffer salt, 0.11% vitamin, 0.05% amino acid, 98.8% water; the buffer salt is sodium chloride, the vitamin is vitamin B2, and the amino acid is proline; The preparation method is as follows: S1, grinding carboxymethyl chitosan into powder after freeze-drying to obtain an essence component; S2, weighing acetyl glucosamine, allantoin, niacinamide, panthenol, carnosine and water, mixing and stirring evenly, then adding buffer salts, vitamins, and amino acids, continuing to mix and stir evenly, and finally adding ectoin, mixing and stirring evenly, to obtain a solvent component; S3. Add the solvent component to the essence component and mix and stir evenly to obtain the hydrating essence. The pH value of the hydrating essence is 7.0.

[0047] Embodiment 2: This embodiment differs from Embodiment 1 in that: The mass ratio of the essence component to the solvent component is 1:100; The essence component is an essence freeze-dried sponge, and the essence freeze-dried sponge is selected from the essence freeze-dried sponge prepared in Preparation Example 4; Solvent components: 5% acetyl glucosamine, 0.2% allantoin, 1.8% niacinamide, 3% panthenol, 3% carnosine, 5% ectoine, 0.8% buffer salt, 1.6% vitamins, 1.6% amino acids, and 78% water; the buffer salt is sodium chloride, the vitamin is vitamin B2, and the amino acid is glycine; the acetyl glucosamine is the carrier acetyl glucosamine prepared in Preparation Example 1.

[0048] Embodiment 3: This embodiment differs from Embodiment 2 in that: The mass ratio of the essence component to the solvent component is 2.5:100; The essence component is an essence freeze-dried sponge, and the essence freeze-dried sponge is selected from the essence freeze-dried sponge prepared in Preparation Example 5; Solvent components: 2.5% acetyl glucosamine, 0.2% allantoin, 1.8% niacinamide, 3% panthenol, 3% carnosine, 5% ectoine, 0.8% buffer salt, 1.6% vitamins, 1.6% amino acids, and 80.5% water; the buffer salt is sodium chloride, the vitamin is vitamin B6, and the amino acid is glycine; the acetyl glucosamine is the carrier acetyl glucosamine prepared in Preparation Example 2.

[0049] During the preparation process: S3. Add the solvent component to the essence component and mix well to obtain the hydrating essence. The pH value of the essence solution is 6.5.

[0050] Embodiment 4: This embodiment differs from Embodiment 2 in that: The mass ratio of the essence component to the solvent component is 1:50; The essence component is an essence freeze-dried sponge, and the essence freeze-dried sponge is selected from the essence freeze-dried sponge prepared in Preparation Example 6; Solvent components: 5% acetyl glucosamine, 0.3% allantoin, 3% niacinamide, 5% panthenol, 5% carnosine, 7% ectoine, 1% buffer salt, 3% vitamins, 3% amino acids, and 67.7% water; the buffer salt is disodium hydrogen phosphate, the vitamin is vitamin B12, and the amino acid is aspartic acid; the acetyl glucosamine is the carrier acetyl glucosamine prepared in Preparation Example 3.

[0051] During the preparation process: S3. Add the solvent component to the essence component and mix well to obtain the hydrating essence. The pH value of the essence is 7.5.

[0052] Embodiment 5: This embodiment differs from Embodiment 2 in that: No modified hydroxyapatite nanowires were added during the preparation of the essence freeze-dried sponge.

[0053] Embodiment 6: This embodiment differs from Embodiment 2 in that: No isopropyl myristate was added during the preparation of modified hydroxyapatite nanowires from the essence freeze-dried sponge.

[0054] Embodiment 7: This embodiment differs from Embodiment 2 in that: During the preparation of modified hydroxyapatite nanowires of essence freeze-dried sponges, hyaluronic acid synthase and carboxymethyl chitosan solution were not added.

[0055] Embodiment 8: This embodiment differs from Embodiment 2 in that: No vitamin C nanoliposomes were added during the preparation of the loaded acetyl glucosamine. Comparative Example

[0056] Comparative Example 1: The difference between this comparative example and Example 1 is that: No acetyl glucosamine was added to the solvent.

[0057] Comparative Example 2: The difference between this comparative example and Example 1 is that: No ectoine was added to the solvent.

[0058] Comparative Example 3: The difference between this comparative example and Example 1 is that: Carnosine and allantoin were not added to the solvent. Performance testing 1. Moisturizing effect test

[0059] The water light needle essence was prepared by using the preparation methods of Examples 1-8 and Comparative Examples 1-3 respectively; Test subjects: 110 healthy skin test subjects with no history of cosmetic allergy were selected, aged 20-25 years old, female, and divided into 11 groups, 10 people in each group; Test method: The test was conducted at room temperature of 21℃±1℃ and humidity of 50%±10%. The subjects first cleaned their faces for the same length of time (cleaned with water first, then thoroughly cleaned with the same brand of facial cleanser). After the skin was naturally dried, 5cm×5cm skin on the cheek was selected as the test area, and 1mL was injected into the skin, 1.5mm subcutaneous; the water content of the stratum corneum was tested at 0d and 7d respectively, and the instrument was a multi-probe skin testing system from German CK Company; the water content of the skin was tested before the injection of water light needle essence, recorded as the basic value (D0), and the water content was recorded after 7d of using the product (D7), and the improvement rate of the water content of the stratum corneum of the skin after 7d of using the product was calculated. 2. Collagen detection

[0060] The water light needle essence was prepared by the preparation methods of Examples 1-5, 8 and Comparative Example 3, respectively. A healthy skin test population with no history of cosmetic allergies was selected, aged 20-25 years old, 110 women, and evenly divided into 11 groups, 10 people in each group; the test was carried out at room temperature of 21°C ± 1°C; humidity of 50% ± 10%. The test subjects first cleaned their faces for the same length of time (first cleansed with water, and then thoroughly cleaned with the same brand of facial cleanser). After the skin was naturally dried, 5cm × 5cm skin on the cheek was selected as the test area, and 1mL was injected into the skin, 1.5mm subcutaneously; the skin two-photon in vivo imaging system SUPERVISION-780 was used to detect the harmonic intensity of collagen fibers. The proportion of collagen fiber area after 0d and 28d was detected, and the growth ratio of collagen fibers was judged by comparing the difference in the harmonic intensity of collagen fibers. 3. Skin elasticity test

[0061] The water light needle essence was prepared by the preparation methods of Examples 1-5, 8 and Comparative Example 3, and healthy skin subjects with no history of cosmetic allergies were selected. The subjects were aged 20-25 years old, 110 women, and were evenly divided into 11 groups, with 10 people in each group. The test was carried out at room temperature of 21°C ± 1°C and humidity of 50% ± 10%. The subjects first cleaned their faces for the same length of time (first cleansed with water, and then thoroughly cleaned with the same brand of facial cleanser). After the skin was naturally dried, 5cm×5cm skin on the cheek was selected as the test area, and 1mL was injected into the skin, 1.5mm subcutaneously; 24h after the injection, the skin elasticity was determined by the skin elasticity tester CutometerdualMPA580, and the MPA software was analyzed to determine the elasticity of the skin. 4. Sensitive skin can be tested

[0062] The preparation methods of Examples 1-8 and Comparative Examples 1-3 were used to prepare water light needle essence, and 110 women aged 20-25 years old with a subjective score of ≥8 points were selected for sensitive skin screening. They were evenly divided into 11 groups, with 10 people in each group. The test was carried out at room temperature of 21°C ± 1°C and humidity of 50% ± 10%. The subjects first cleaned their faces for the same length of time (first cleansed with water, and then thoroughly cleaned with a facial cleanser of the same brand), and after the skin was naturally dried, 5cm×5cm skin on the cheek was selected as the test area, and 1mL was injected into the skin, 1.5mm subcutaneously; 1h after the injection, the doctor conducted an adverse reaction assessment according to the following standards; Human trial skin reaction grading standards: No response – Level 0; Faint erythema - grade 1; Erythema, infiltration, papules – Grade 2; erythema, water, papules, blisters - grade 3; Erythema, watery, bullous - Grade 4. 5. Repair performance test

[0063] The preparation methods of Examples 1-4 were used to prepare the water-light needle essence, and the water-light needle essence was injected with a needle; the subjects first cleaned their faces for the same length of time (first with clean water, and then thoroughly cleaned with the same brand of facial cleanser), and after the skin was naturally dried, 5 cm × 5 cm skin on the cheek was selected as the test area, and 1 mL was injected into the skin, 1.5 mm subcutaneously; a transepidermal water loss tester Vapometer was used to detect the transepidermal water loss rate (TEWL) of the skin after 0d and 28d, and the improvement rate of transepidermal water loss of the skin after using the product was calculated.

[0064] Table 1 Performance test table (“ / ” in the table means that the corresponding embodiment or comparative example did not test the item, so there is no data)

[0065] Combining Example 1 and Examples 2-4 with Table 1, it can be seen that after acetyl glucosamine is loaded with vitamin C nanoliposomes, the improvement rate of the moisture content of the skin stratum corneum in Example 2 is better than that in Example 1, the collagen fiber growth rate is greater than that in Example 1, the elastic performance is greater than that in Example 1, and the improvement rate of transepidermal water loss is greater than that in Example 1; this indicates that vitamin C nanoliposomes can promote the synthesis of collagen in the deep layer of the skin, improve skin elasticity and flexibility, and the liposomes have a good moisturizing effect, further ensuring the water content of the skin; at the same time, the skin barrier layer is better repaired after using the water light needle essence for 28 days.

[0066] Combining Example 2 and Examples 5-8 and Table 1, it can be seen that modified hydroxyapatite nanowires are not added to the essence freeze-dried sponge of Example 5. Compared with Example 2, the improvement rate of the moisture content of the skin stratum corneum of Example 5 is worse than that of Example 1, the collagen fiber growth rate is less than that of Example 2, and the elastic performance is lower than that of Example 2; this shows that the addition of hydroxyapatite nanowires can stimulate and promote the formation of collagen, and the modified hydroxyapatite nanowires, sodium hyaluronate, collagen, and carboxymethyl chitosan solution can form an interwoven network in the skin, further increasing the contact area and contact rate of cells, regulating cell proliferation and differentiation, and repairing damaged skin cells, improving skin elasticity and tenderness, and prolonging the moisturizing effect time of the water light needle essence.

[0067] In the preparation process of the modified hydroxyapatite nanowires in Example 6, isopropyl myristate was not added, and in the preparation process of the modified hydroxyapatite nanowires in Example 7, hyaluronic acid synthase and carboxymethyl chitosan solution were not added. Compared with Example 2, the improvement rate of the water content of the skin stratum corneum in Examples 6 and 7 was slightly lower than that in Example 2; this indicates that isopropyl myristate has a better penetration effect, which facilitates the contact of the modified hydroxyapatite nanowires with the deep layer of the skin, and utilizes the hyaluronic acid synthase on the surface of the hydroxyapatite nanowires to promote the synthesis of hyaluronic acid in the deep skin, thereby improving the repair effect of the deep layer of the skin, reducing the formation of wrinkles, and ensuring the elasticity and firmness of the skin; and carboxymethyl chitosan can also have a repair effect in the dermis of the skin and promote cortical repair.

[0068] In the preparation process of Example 8 loading acetyl glucosamine, no vitamin C nanoliposomes were added. Compared with Example 2, the improvement rate of the moisture content of the skin stratum corneum in Example 8 was worse than that in Example 1, and the collagen fiber growth rate was less than that in Example 2. This indicates that the addition of vitamin C nanoliposomes, along with the slow release of vitamin C, promotes the gradual synthesis of collagen in the deep layer of the skin, thereby increasing the collagen content in the deep layer of the skin, and in combination with the essence components, improves the softness, elasticity and firmness of the skin, and improves the moisturizing effect.

[0069] Combining Example 1 and Comparative Examples 1-3 and Table 1, it can be seen that no acetyl glucosamine is added to the solvent of Comparative Example 1. Compared with Example 1, the moisturizing effect of the water light needle essence prepared in Comparative Example 1 is worse than that in Example 1, which indicates that the small molecules of acetyl glucosamine can easily penetrate into the skin, thereby improving the moisturizing effect.

[0070] No ectoine was added to the solvent of Comparative Example 2. Compared with Example 1, the moisturizing and water-retaining effects of Comparative Example 2 were worse than those of Example 1, indicating that ectoine can moisturize the skin for a long time and replenish skin moisture.

[0071] No carnosine and allantoin were added to the solvent of Comparative Example 3. Compared with Example 1, the difference in collagen content of the essence prepared in Comparative Example 3 was less than that in Example 1, and the elasticity parameter was less than that in Example 1; this indicates that carnosine and allantoin cooperate to stimulate collagen production and increase skin elasticity and firmness.

[0072] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A water light needle essence, characterized in that: Containing an essence component and a solvent component in a mass ratio of 0.5-10:100; The essence component is selected from any one of essence freeze-dried powder, essence freeze-dried sponge or essence solid dressing; The solvent component comprises the following raw materials in weight percentage: 0.05%-5% acetyl glucosamine, 0.05%-0.3% allantoin, 0.11%-3% niacinamide, 0.11%-5% panthenol, 0.11%-5% carnosine, 0.11%-7% ectoine, 0.5%-1% buffer salt, 0.11%-3% vitamins, 0.05%-3% amino acids, and the balance is water.

2. The water light needle essence according to claim 1, characterized in that: The buffer salt is one or more of sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium acetate, ferrous sulfate, zinc sulfate, magnesium sulfate, disodium hydrogen phosphate, and sodium dihydrogen phosphate.

3. The water light needle essence according to claim 1, characterized in that: The vitamins are selected from one or more of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B8, vitamin B9, vitamin B12, vitamin A, vitamin C, and vitamin E.

4. The water light needle essence according to claim 1, characterized in that: The amino acids are selected from one or more of alanine, glycine, proline, valine, serine, lysine, tyrosine, threonine, tryptophan, phenylalanine, ornithine, arginine, asparagine, glutamic acid, histidine, isoleucine, leucine, aspartic acid, cystine, glutamine, hydroxyproline, and cysteine.

5. The water light needle essence according to claim 1, characterized in that: The acetyl glucosamine is a carrier acetyl glucosamine, and the carrier is made of vitamin C nano liposomes, sodium alginate solution and acetyl glucosamine in a mass ratio of 1:0.2-0.5:1-2.

6. The water light needle essence according to claim 1, characterized in that: The essence freeze-dried powder is selected from one or more of sodium hyaluronate freeze-dried powder, collagen freeze-dried powder, carboxyalkyl chitosan freeze-dried powder, carboxyalkyl chitin freeze-dried powder, hydroxyalkyl chitosan freeze-dried powder, hydroxyalkyl chitin freeze-dried powder and succinyl chitosan freeze-dried powder.

7. The water light needle essence according to claim 1, characterized in that: The essence freeze-dried sponge is prepared from sodium hyaluronate, collagen, modified hydroxyapatite nanowires and carboxymethyl chitosan solution in a mass ratio of 1:0.5-1:0.05-0.1:0.5-1.

8. The water light needle essence according to claim 7, characterized in that: The modified hydroxyapatite nanowires are prepared by adsorbing isopropyl myristate onto the hydroxyapatite nanowires and then bonding them with a carboxymethyl chitosan solution and hyaluronic acid synthase; The mass ratio of hydroxyapatite nanowires, carboxymethyl chitosan solution and hyaluronic acid synthase is 1:0.1-0.2:0.05-0.

1.

9. The method for preparing the water light needle essence according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1, preparing essence components; S2, weighing acetyl glucosamine, allantoin, niacinamide, panthenol, carnosine and water, mixing and stirring evenly, then adding buffer salts, vitamins, and amino acids, continuing to mix and stir evenly, and finally adding ectoin, mixing and stirring evenly, to obtain a solvent component; S3. Add the solvent component to the essence component and mix well. The pH should be 6.0-8.0 to obtain the hydrating essence.

Images