Heterochromatin protein HP1 and histone assembled protein complex, preparation method and antinuclear antibody screening kit
By constructing and expressing histone H2A-H2B and HP1 protein C-terminal truncates, forming a protein complex and applying it to an anti-nuclear antibody screening kit, the problem of the interaction between heterochromatin protein HP1 and histones is solved, and the accuracy of autoimmune disease diagnosis is improved.
CN119912581APending Publication Date: 2025-05-02SHENZHEN DYMIND BIOTECH
View PDF 0 Cites 0 Cited by
Patent Information
- Application Number
- CN202311447546.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-10-31
- Publication Date
- 2025-05-02
Smart Images
Figure CN119912581A_ABST
Abstract
The invention provides a heterochromatin protein HP1 and histone H2A-H2B assembled protein complex, a preparation method and an antinuclear antibody screening kit, the protein complex is formed by combining the histone H2A-H2B and an HP1 protein C-terminal truncation, and the selection region of the HP1 protein C-terminal truncation is 109-175 AA; according to the invention, the HP1-CSD and the histone H2A-H2B are assembled in vitro, the C-terminal truncated body of the heterochromatin protein HP1 or the HP1 protein can be directly combined with the histone H2A-H2B, and more theoretical references are provided for subsequent research on a molecular mechanism formed by the HP1-mediated heterochromatin. In addition, the invention provides an antinuclear antibody screening kit, the kit comprises the protein complex, and the reagent stability is improved.
Need to check novelty before this filing date? Find Prior Art