Method for rapidly collecting momordica grosvenori pollen and pollinating momordica grosvenori pollen

Abstract

The invention discloses a method for rapidly collecting momordica grosvenori pollen and pollinating the momordica grosvenori pollen. The method for rapidly collecting the fructus momordicae pollen comprises the following steps: step 1, pouring stamens of fructus momordicae male flowers into a mesh screen, and then adding lycopodium powder to be uniformly mixed with the stamens; 2, shaking the mesh screen to enable the pollen of the stamens to fall off along with the lycopodium powder, and pouring the screened mixed powder containing the pollen and the lycopodium powder back to the mesh screen; and repeating the step 2 until no obvious pollen cubes exist in the anther chambers on the stamens. According to the method for rapidly collecting the pollen of the momordica grosvenori and pollinating the momordica grosvenori, the problems that in the prior art, in spray pollination of the momordica grosvenori, the pollen collection treatment time is long, the pollen collection speed is low, and the pollen collection steps are tedious and are not easily accepted and popularized by farmers are solved, and the pollination efficiency of the momordica grosvenori is improved; the implementation and popularization of the artificial pollination of the momordica grosvenori can be accelerated.

Description

Technical Field

[0001] The present application belongs to the technical field of monk fruit, and specifically relates to a method for quickly collecting monk fruit pollen and pollinating the same. Background Art

[0002] Monk fruit (Siraitia grosvenorii (Swingle) C. Jeffrey) is a perennial vine of the genus Momordica in the Cucurbitaceae family. Monk fruit is a famous specialty of Guangxi and one of my country's traditional export commodities. It has a long-standing reputation in Southeast Asia, Japan, Europe and the United States, and is known as the "Oriental Magic Fruit". The market is in short supply. Due to its dry and cool nature, it has the effects of moistening the lungs and relieving coughs, clearing heat and cooling blood, relieving heat, and moistening the intestines and facilitating bowel movements. It is widely used in the fields of medicine, beverages, and condiments.

[0003] Since Momordica grosvenori is a dioecious plant, the natural pollination rate of female flowers is extremely low, and cultivation must rely on artificial assisted pollination to produce fruit. Two people in a farmer's household can only pollinate 500 plants (500 female flowers) of Momordica grosvenori every day using the traditional artificial pollination method. The flowering period of Momordica grosvenori is relatively long, and artificial pollination every day during flowering has become a routine and heavy technical work in Momordica grosvenori production.

[0004] Mechanically assisted pollination of common plants such as kiwifruit, apples, and pears usually involves drying pollen to form powder, filling it with auxiliary materials, and then mechanically pollinating. Patent application publication number CN109618914A discloses a method for spraying pollination of monk fruit and a method for preparing pollen using this method. The advantage of the method disclosed in patent application publication number CN109618914A is that the pollination speed is faster than traditional artificial flower pollination, which improves the efficiency of Momordica grosvenori spray pollination. However, Momordica grosvenori male flowers are usually scattered on various branches and vines, and the stamens are located under the petals, the anthers are laterally "S"-shaped, and the pollen is sticky and clumped. There are large differences in flower structure and pollen texture from common dicotyledonous plants. There are long times to pick flowers from Momordica grosvenori male plants, long times to collect anthers from flowers to make pollen (because pollen needs to be dried with a desiccant for 24-48 hours, and the desiccant needs to be replaced every 6 to 8 hours during this period to obtain dry pollen for spray pollination), and the pollen preparation method is cumbersome. The problem is that it is not easy to be mastered and accepted by Momordica grosvenori farmers, resulting in difficulties in promoting Momordica grosvenori spray pollination technology.

[0005] Therefore, a method for simply and quickly collecting Momordica grosvenori pollen is needed to overcome the shortcomings of the prior art. Summary of the invention

[0006] In view of the problems existing in the prior art, after many experiments and practices, the present application invented for the first time a method for quickly collecting monk fruit pollen and pollinating monk fruit.

[0007] Specifically, this application involves the following contents:

[0008] 1. A method for quickly collecting Momordica grosvenori pollen, comprising:

[0009] Step 1: Pour the stamens of the male flowers of Monk Fruit into a mesh sieve, then add Lycopodium powder and mix well;

[0010] Step 2: Shake the sieve to make the pollen of the stamens fall off with the lycopodium powder, and pour the sieved mixed powder containing pollen and lycopodium powder back into the sieve;

[0011] Then repeat step 2 until there are no obvious pollen blocks in the anther chambers on the stamens.

[0012] 2. The method according to item 1, wherein, before step 1, the method further comprises: collecting male and female plants of Momordica grosvenori;

[0013] Preferably, the male and female plants of Momordica grosvenori are selected and collected under conditions such that the flowering period of the male plants of Momordica grosvenori is 3-10 days earlier than the flowering period of the female plants;

[0014] Preferably, the collected male and female Momordica grosvenori plants are collected under the condition that at least 2 male plants are planted for every 100 female Momordica grosvenori plants;

[0015] Preferably, male plants are arranged around the plots of female plants and are provided with fence-like climbing. During the growth of male plants, side buds are not removed. When they grow to 1 meter in height, they are topped to promote budding and flowering, and at the same time, the supply of fertilizer and water is increased.

[0016] 3. The method according to item 2, wherein the method further comprises: picking male flowers during the flowering period of male Momordica grosvenori plants, and then cutting off the stamens of the male flowers; preferably, placing the stamens at a temperature of ≤35°C before using them in step 1.

[0017] 4. The method according to item 1, wherein in step 1, the mass ratio of stamens to lycopodium powder is (2-8):1; preferably, the mesh size of the sieve is 30-50 meshes.

[0018] 5. The method according to item 1, wherein in step 2, the pollen and lycopodium powder of the stamens are brushed off with a brush while the mesh screen is shaken.

[0019] 6. The method according to item 1, wherein step 2 is repeated 2-3 times;

[0020] Preferably, the mixed powder containing pollen and lycopodium powder sieved out in step 2 is stored at 1-10°C, preferably 3-5°C, and then used for pollination of female flowers.

[0021] 7. A method for quickly collecting and pollinating Momordica grosvenori pollen, comprising:

[0022] The method comprises collecting pollen of male flowers of Momordica grosvenori by any one of items 1 to 6; and pollinating the collected pollen of male flowers to the stigma of female flowers.

[0023] 8. The method according to item 7, wherein the collected mixed pollen containing male flower pollen and lycopodium powder is directly pollinated on the stigma of female flowers.

[0024] Beneficial effects of this application:

[0025] 1) According to the prior art (such as CN109618914A), the anthers of Momordica grosvenori need to be dried at room temperature for 24 to 48 hours and the desiccant needs to be replaced every 6 to 8 hours. In the present application, the stamens of Momordica grosvenori can be directly sieved to obtain the pollen of male flowers without being treated with a desiccant or drying, thereby saving 24 to 48 hours. The method is simple, time-saving, and trouble-free, and is easily accepted by farmers.

[0026] 2) When the male and female Momordica grosvenori plants are planted at the same time in the present application, the flowering time of the male plants is 5-10 days earlier than that of the female plants, which is conducive to ensuring that the pollen of the male flowers collected in the present application can be in the flowering period of the female plants.

[0027] 3) In the present application, by planting male plants in a concentrated manner around the female plant plot and setting up a fence-like climbing fence, the side buds are not removed during the growth process, and the plants are topped when they grow to 1 meter in height to promote budding and flowering. This can make the flowering nodes low and the flowering positions concentrated. A large number of male flowers can be easily picked in a short time without occupying land, thereby achieving the effect of quickly picking and collecting male flowers, thereby solving the problem of time-consuming and laborious collection of a large number of male flowers.

[0028] The method for quickly collecting monk fruit pollen and pollination thereof of the present application solves the problems that the pollen in the spray pollination method and pollen preparation method of patent application CN109618914A needs to be dried with a desiccant for 24 to 48 hours, resulting in a long collection and processing time, a slow pollen collection speed, a low pollen collection efficiency, and a cumbersome pollen collection process that is not easily accepted and promoted by farmers. At the same time, the present application uses fresh pollen for spray pollination, which maintains the vitality of the pollen, improves the effect of monk fruit pollination, and helps to accelerate the promotion of spray pollination technology in monk fruit producing areas. DETAILED DESCRIPTION

[0029] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0030] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0031] On the one hand, the present application provides a method for quickly collecting Momordica grosvenori pollen, the method comprising the following steps:

[0032] Step 1: Pour the stamens of the male flowers of Monk Fruit into a mesh sieve, then add Lycopodium powder and mix well;

[0033] Step 2: Shake the sieve to make the pollen of the stamens fall off with the lycopodium powder, and pour the sieved mixed powder containing pollen and lycopodium powder back into the sieve;

[0034] Then repeat step 2 until there are no obvious powder blocks in the anther chambers on the stamens.

[0035] The lycopodium powder in the above step 1 can be commercially available (dyed) lycopodium spore powder, which is obtained by drying the spores of Lycopodiaceae plants. It is in powder form, light in weight, has good hydrophobic properties, and can easily slide off with a slight shake in the sieve, taking away the pollen in the stamens, thereby achieving the purpose of evenly dispersing the pollen.

[0036] The stamens in the above step 1 can be freshly picked stamens, or stamens stored in a cool and dry place. As long as the stamens effectively maintain the vitality of the pollen, they can be used.

[0037] The lycopodium powder used in this application can quickly separate the pollen of the male flowers of Momordica grosvenori and indicate the pollinated flowers by color during the pollination process, increase the pollen volume, improve the pollen fluidity, and increase the pollen biocompatibility, and is suitable for mechanical powder spraying of Momordica grosvenori. However, flour, corn flour, talcum powder, etc., which are not used in this application, are easy to absorb water and agglomerate, have too high a specific gravity, poor fluidity, and are not suitable for mechanical powder spraying.

[0038] In some embodiments of the present application, in the above-mentioned method for quickly collecting monk fruit pollen, before step 1, the method further includes: collecting male and female monk fruit plants; preferably, collecting male and female monk fruit plants makes the flowering period of male monk fruit plants 3-10 days earlier than the flowering period of female plants, so as to ensure that the pollen of male flowers is collected in the flowering period of female plants, and more preferably, the male plant variety can be the excellent male plant strain selected and promoted by Giffords, such as "excellent male plant strain 0009"; the female plant variety can be The "Earth" series of monk fruit excellent varieties and excellent strains selected by Giffords Company; preferably, the male and female monk fruit collected are collected under the condition that more than 2 male plants are planted for every 100 female monk fruit plants, wherein the male plants are set around the female plant plots, and a fence-style climbing is set. During the growth of the male plants, the side buds can be removed without wiping them. When they grow to 1 meter high, they are topped to promote budding and flowering. At the same time, the supply of fertilizer and water is strengthened. This practice can make the flowering nodes low and the flowering positions concentrated, so that a large number of male flowers can be easily picked in a short time without occupying land. The above-mentioned fence-style climbing refers to using fences as climbing objects to make male plants grow around the fences. The above-mentioned not wiping side buds refers to not removing side buds, so that the flowering nodes are low and the flowering positions are concentrated, so that a large number of male flowers can be easily picked in a short time without occupying land.

[0039] In some embodiments of the present application, in the above-mentioned method for quickly collecting Momordica grosvenori pollen, the method further includes: picking male flowers during the flowering period of male Momordica grosvenori plants, wherein more than 1,000 male flowers can be picked in 1 hour, and then cutting off the stamens with tools such as scissors; preferably, the stamens are placed at a temperature of ≤35°C (e.g., 3°C, 6°C, 10°C, 15°C, 20°C, 25°C, 30°C, 35°C), more preferably placed in a cool and dry environment of ≤35°C, and then used for subsequent steps, such as pouring it into a mesh sieve and then mixing it with Lycopodium powder (i.e., step 1). Compared with the male flowers climbing on the horizontal trellis of traditional scattered planting, it takes more than 1.5 hours to pick 1,000 male flowers, which is time-consuming and laborious. The planting method of the above-mentioned male plants in the present application allows more than 1,000 male flowers to be picked within 1 hour. In addition, the present application places the cut stamens at a temperature of ≤35°C, which can effectively maintain the pollen vitality of the male flowers.

[0040] In some embodiments of the present application, in the above-mentioned method for quickly collecting monk fruit pollen, the mass ratio of stamens to lycopodium powder is (2-8): 1, for example, 2: 1, 3: 1, 4: 1, 5: 1, 6: 1, 7: 1, 8: 1. If the mass ratio of stamens to lycopodium powder is less than 2: 1, the pollen is not easy to disperse into a mass, and if the mass ratio of stamens to lycopodium powder is greater than 8: 1, the amount of pollen in the mixed pollen is too little to affect the success rate of pollination.

[0041] In some embodiments of the present application, in the above-mentioned method for quickly collecting monk fruit pollen, in step 1, the sieve hole size is 30-50 mesh (for example, 32 mesh, 34 mesh, 36 mesh, 38 mesh, 40 mesh, 42 mesh, 44 mesh, 46 mesh, 48 mesh). If the sieve hole is too large, the pollen is unevenly dispersed. If the sieve hole is too small, the mixed pollen is screened slowly.

[0042] In some embodiments of the present application, in the above-mentioned method for quickly collecting monk fruit pollen, in step 2, while shaking the mesh screen, a brush is used to brush off the pollen and lycopodium powder of the stamens; here, by using a brush, the pollen and lycopodium powder of the stamens can be sifted off the mesh screen faster.

[0043] In some embodiments of the present application, in the above method for quickly collecting monk fruit pollen, step 2 is repeated 2-3 times until there is no obvious powder block in the anther chamber on the stamen. The number of repetitions of step 2 can also be appropriately increased, but considering the time required to sift out the pollen, it is preferred that step 2 be repeated no more than 4 times.

[0044] In some embodiments of the present application, in the above-mentioned method for quickly collecting monk fruit pollen, the mixed powder containing pollen and lycopodium powder sieved out in step 2 is stored at 1-10°C (e.g., 2°C, 4°C, 6°C, 8°C or 9°C), preferably at 3-5°C, to maintain pollen vitality, and then used for pollination of female flowers.

[0045] On the other hand, the present application also provides a method for quickly collecting and pollinating monk fruit pollen, the method comprising: collecting pollen from monk fruit male flowers by the above method; and pollinating the collected male flower pollen onto the stigma of a female flower.

[0046] In some embodiments of the present application, in the above-mentioned method for rapid collection and pollination of monk fruit pollen, the collected mixed pollen containing male flower pollen and lycopodium powder is directly pollinated on the female flower stigma, for example, the mixed pollen containing male flower pollen and lycopodium powder can be directly dipped into the female flower stigma by using a pollination pen, or the mixed pollen containing male flower pollen and lycopodium powder is loaded into a manual duster or an electric duster for pollination on the female flower stigma. Because the amount of mixed pollen used by the pollination pen for pollination is reduced by about 50% compared with the duster, the time for making mixed pollen is saved, and the pollination speed of the duster is faster than that of the pollination pen, so the pollination pen and the duster can achieve substantially the same number of pollinated plants at the same time; preferably, pollination is carried out all day on cloudy or light rainy days, and pollination is carried out before 11 am on sunny days, so as to avoid the influence of high temperature and improve the success rate of spray pollination. One farmer can complete the pollination of 1,500 female plants (15,000 female flowers) before 11 am on a sunny day. In this embodiment, the mixed pollen containing male flower pollen and lycopodium powder can be directly pollinated on the stigma of female flowers without the need for additional intermediate steps, thereby making the steps simpler.

[0047] Example 1

[0048] The method for quickly collecting Momordica grosvenori pollen and pollination thereof of the present embodiment comprises the following steps:

[0049] (1) Steps for setting up female and male plants in a field of Momordica grosvenori

[0050] When the temperature rises steadily to 15-18℃ in spring, female Momordica grosvenori plants are planted at a density of 130-150 plants / mu, and male plants are concentrated around the female plant plots, among which 4 male plants are set for every 100 female Momordica grosvenori plants. The male and female Momordica grosvenori plants are selected so that the flowering period of male Momordica grosvenori plants is 5-10 days earlier than that of female plants. In addition, the male plants are set up with fence-like climbing. During the growth of the male plants, the side buds do not need to be removed. When they grow to 1 meter high, they are topped to promote budding and flowering, and at the same time, the supply of fertilizer and water is strengthened.

[0051] (2) Steps for quickly collecting male flowers of Momordica grosvenori

[0052] In the morning when the male flowers of Momordica grosvenori bloom, pick the male flowers quickly. More than 1,000 male flowers can be picked in one hour (one hand holding male flowers is approximately equal to 100 flowers), and place them in a cool and ventilated place for processing.

[0053] (3) Steps for collecting stamens

[0054] The male flowers picked in the above (2) are plucked with scissors to remove the stamens, and the stamens are spread out in a smooth and clean container and placed in a cool and dry environment at ≤35°C.

[0055] (4) Steps for rapid separation of male flower pollen from Momordica grosvenori

[0056] Prepare clean monk fruit mesh sieve, pollen brush, and lycopodium powder. The monk fruit mesh sieve is a stainless steel sieve with a mesh size of 40 mesh and a mesh frame diameter of 10 cm, including 1 bottom plate, 1 mesh body, and 1 sieve cover. Use a soft brush for the pollen brush.

[0057] The stamens collected in (3) above can be directly poured into the sieve without being treated with a desiccant or drying. Then, add Lycopodium powder in a weight ratio of 10 g:2 g of stamens to Lycopodium powder, and mix well with the stamens → shake the sieve to make the pollen of the stamens fall off with the Lycopodium powder, and use a brush to brush off the pollen and Lycopodium powder on the main body of the sieve. Pour the sieved pollen and Lycopodium powder mixture back into the sieve → continue mixing and sieving, and repeat the above steps 2-3 times until there are no obvious pollen blocks in the anther chamber.

[0058] (5) Steps of pollination of monk fruit

[0059] Use a pollination pen to dip the fresh mixed pollen sifted in (4) above into the stigma of the female flower. Pollination can be done all day on cloudy days, and before 11 a.m. on sunny days.

[0060] The present embodiment is for pollination by dipping in Momordica grosvenori production areas such as Lingui District, Longsheng County, and Ziyuan County from 2020 to 2023. The traditional artificial flower-pointing method is to use a sharpened bamboo stick to dig pollen from the anther chamber of the male flower to touch the female flower stigma to complete pollination. On average, one male flower can only pollinate 10-20 female flowers. One farmer can only pollinate 500 female plants (500 female flowers) per day. Relatively speaking, using the method of the embodiment of the present application, one farmer can pollinate 1500 plants (1500 female flowers) per day. In the present embodiment, the ovary of Momordica grosvenori significantly swells 2-3 days after pollination, and the young fruit of Momordica grosvenori rapidly swells and grows 15 to 25 days after pollination. The fruit completes the expansion growth link 35 days after pollination, and the fruit matures 85 days after pollination. The fruit is oval, which is consistent with the growth and development of the fruit of traditional artificial flower-pointing pollination. The pollination success rate of this embodiment is more than 80% (wherein, the pollination success rate refers to the proportion of fruits after pollination, for example, a pollination success rate of 80% means that 80 flowers out of 100 flowers bear fruits after pollination (the fruits are that the ovaries are significantly enlarged)), which is no different from the pollination success rate of artificial flower spotting.

[0061] Example 2

[0062] The method for quickly collecting Momordica grosvenori pollen and pollination thereof of the present embodiment comprises the following steps:

[0063] (1) Steps of setting female and male plants in Momordica grosvenori field: Same as Example 1

[0064] (2) Steps for rapid collection of Momordica grosvenori male flowers: same as in Example 1

[0065] (3) Step of collecting stamens: same as in Example 1

[0066] (4) Steps for rapid separation of Momordica grosvenori male flower pollen: same as in Example 1

[0067] (5) Steps of pollination of monk fruit

[0068] The fresh mixed powder containing male flower pollen and lycopodium powder sieved in the above (4) is loaded into an electric duster for pollination on the stigma of the female flower. Pollination can be done all day on cloudy days, and pollination can be done before 11 am on sunny days.

[0069] During the flowering period of Momordica grosvenori, the above (2)-(5) are repeated in sequence until the expected Momordica grosvenori yield is obtained. This embodiment is for spray pollination in Momordica grosvenori producing areas such as Lingui District, Longsheng County, and Ziyuan County from 2020 to 2023. The traditional artificial flower spotting method is to use a sharpened bamboo stick to dig pollen from the anther chamber of the male flower to touch the female flower stigma to complete pollination. On average, one male flower can only pollinate 10-20 female flowers. One farmer can only pollinate 500 plants (500 female flowers) of female plants every day. Using the method of this embodiment, one farmer can pollinate 1500 plants (1500 female flowers) every day. In this embodiment, the ovary of Momordica grosvenori significantly swells 3-5 days after pollination, and the Momordica grosvenori young fruit rapidly swells and grows 15 to 25 days after pollination. The fruit completes the expansion growth link 35 days after pollination, and the fruit matures 85 days after pollination. The fruit is oval, which is consistent with the growth and development of the fruit of traditional artificial flower spot pollination. The pollination success rate of this embodiment is over 80%, which is no different from the pollination success rate of artificial flower spotting.

[0070] Embodiment 3-8

[0071] In Examples 3-8, except for the weights of stamens and lycopodium powder being different from those in Example 1, the rest are the same as those in Example 1.

[0072] The weights of the stamens and lycopodium powder of Examples 1-8 and the obtained conclusions are shown in Table 1 below.

[0073] Table 1

[0074]

[0075] In the above Table 1, the pollination success rate refers to the ratio of the number of female flowers that bear fruit after pollination to the total number of pollinated female flowers.

[0076] “The time required to sift out pollen needs to be increased” refers to the time required to increase the time required to sift out pollen relative to that in Example 2.

[0077] Comparative Example 1

[0078] In this comparative example, corn flour was used to replace the lycopodium powder in Example 1 and mixed with the stamens, and the remaining steps were the same as those in Example 1. The results were as follows: corn flour had good water absorption and was relatively wet, which caused the pollen to clump together and affected the fluidity of the pollen.

[0079] Comparative Example 2

[0080] In this comparative example, talcum powder was used to replace the lycopodium powder in Example 1 for mixing with the stamens, and the remaining steps were the same as those in Example 1. The results were as follows: talcum powder was heavier than pollen, and mixing was not good, which affected the fluidity of the pollen.

[0081] The above-mentioned technical scheme only reflects the preferred technical scheme of the technical scheme of the present application. Some changes that may be made to certain parts thereof by technicians in this technical field all reflect the principles of the present application and fall within the protection scope of the present application.

Claims

1. A method for quickly collecting Momordica grosvenori pollen, characterized in that: include: Step 1: Pour the stamens of the male flowers of Monk Fruit into a mesh sieve, then add Lycopodium powder and mix well; Step 2: Shake the sieve to make the pollen of the stamens fall off with the lycopodium powder, and pour the sieved mixed powder containing pollen and lycopodium powder back into the sieve; Then repeat step 2 until there are no obvious pollen blocks in the anther chambers on the stamens.

2. The method according to claim 1, characterized in that Before step 1, the method further comprises: collecting male and female Momordica grosvenori plants; Preferably, the male and female plants of Momordica grosvenori are selected so that the flowering period of the male plants of Momordica grosvenori is 3-10 days earlier than the flowering period of the female plants; Preferably, the collected male and female Momordica grosvenori plants are collected under the condition that at least 2 male plants are planted for every 100 female Momordica grosvenori plants; Preferably, male plants are arranged around the plots of female plants and are provided with fence-like climbing. During the growth of male plants, side buds are not removed. When they grow to 1 meter in height, they are topped to promote budding and flowering, and at the same time, the supply of fertilizer and water is increased.

3. The method according to claim 2, characterized in that The method further comprises: picking male flowers during the flowering period of male Momordica grosvenori plants, and then cutting off the stamens of the male flowers; preferably, placing the stamens at a temperature of ≤35° C. and then using them in step 1.

4. The method according to claim 1, characterized in that In step 1, the mass ratio of stamens to lycopodium powder is (2-8):1; preferably, the mesh size of the sieve is 30-50 meshes.

5. The method according to claim 1, characterized in that In step 2, while shaking the mesh screen, the pollen and lycopodium powder of the stamens are brushed off with a brush.

6. The method according to claim 1, characterized in that Repeat step 2 2-3 times; Preferably, the mixed powder containing pollen and lycopodium powder sieved out in step 2 is stored at 1-10°C, preferably 3-5°C, and then used for pollination of female flowers.

7. A method for quickly collecting and pollinating Momordica grosvenori pollen, characterized in that: include: Collecting pollen from male flowers of Momordica grosvenori by the method according to any one of claims 1 to 6; And the step of pollinating the collected pollen of male flowers onto the stigma of female flowers.

8. The method according to claim 7, characterized in that The collected mixed pollen containing male flower pollen and lycopodium powder is directly pollinated on the stigma of female flowers.

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