A hangover-relieving composition containing GABA and its preparation method and application

Abstract

The present invention discloses a GABA-containing hangover-relief composition, its preparation method, and application, belonging to the field of food technology. The present invention provides a GABA-containing hangover-relief composition, comprising the following components: gamma-aminobutyric acid, kudzu root extract, Hovenia dulcis fruit extract, vitamin B1, vitamin B6, black plum extract, Imperata cylindrica root extract, corn oligopeptide, taurine, baicalin, and isorhamnetin. The composition can effectively alleviate the damage caused by alcohol to the human body.

Description

Technical Field

[0001] The present invention belongs to the field of food technology, and in particular relates to a hangover-relieving composition containing GABA, and a preparation method and application thereof. Background Art

[0002] Wine is a pillar of Chinese culture, imbued with rich historical significance, and a common beverage at gatherings, dining, and banquets. However, excessive drinking can lead to alcohol poisoning, which can be very harmful to the body. The mechanisms by which alcohol causes intoxication include the following: First, when a large amount of alcohol is consumed in a short period of time, the body is unable to metabolize it quickly, resulting in ethanol accumulation. Increased ethanol levels in the blood significantly suppress the central nervous system, leading to a state of intoxication such as drowsiness or coma. Second, alcohol is broken down into acetaldehyde, which is then broken down into acetic acid by the ALDH enzyme. However, ALDH enzyme activity varies greatly from person to person. In those with low ALDH activity, acetaldehyde cannot be rapidly metabolized into acetic acid, but instead remains in the body, causing symptoms of intoxication such as nausea, vomiting, and unconsciousness. Furthermore, acetaldehyde is a muscle toxin that can damage the liver. Third, when the human body ingests a large amount of ethanol, the high ethanol concentration will induce the enhanced activity of the recombinant protein of cytochrome P450 family member 2E1 (recombinant cytochrome P450 2E1, CYP2E1). At this time, ethanol will be mainly metabolized by CYP2E1 and produce a large number of oxygen free radicals, which can consume a large number of reducing protective substances, cause lipid peroxidation reactions in tissues, and ultimately lead to liver damage.

[0003] Gamma-aminobutyric acid (GABA) is a naturally occurring non-protein amino acid that functions as an important inhibitory neurotransmitter in the human central nervous system. It mitigates the effects of alcohol on the body through the following mechanisms: First, it reduces the burden on the liver. Alcohol is primarily metabolized in the liver, where most alcohol is first converted to acetaldehyde by alcohol dehydrogenase (ADH), then to acetic acid by acetaldehyde dehydrogenase (ALDH), ultimately breaking down into carbon dioxide and water for excretion. GABA can regulate the metabolic state of liver cells and enhance their antioxidant capacity. Second, it promotes the activity of metabolic enzymes. GABA may regulate the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase in the liver. Although the specific molecular mechanisms are not fully understood, some studies suggest that GABA can increase the activity of these metabolic enzymes to a certain extent, accelerating the metabolic breakdown of alcohol in the liver. Third, it relieves gastrointestinal spasms. Alcohol is irritating to the gastrointestinal tract and can easily cause spasms of the smooth muscles of the gastrointestinal tract. GABA acts on receptors on gastrointestinal smooth muscle to regulate its contraction. It relaxes spasmodic smooth muscles, alleviating gastrointestinal discomfort such as abdominal pain and nausea. Fourthly, GABA protects the gastrointestinal mucosa by promoting the repair and regeneration of mucosal cells. Alcohol damages the gastrointestinal mucosa and impairs its barrier function. By regulating growth factors and intercellular signaling pathways in mucosal cells, GABA helps repair damaged mucosa, enhances its defenses, and reduces further damage to the gastrointestinal tract caused by alcohol.

[0004] Therefore, it is very necessary to develop a hangover-relieving composition containing GABA. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a GABA-containing hangover-relief composition that can effectively alleviate the damage caused by alcohol to the human body in response to the existing technology.

[0006] To achieve the above-mentioned object of the invention, the present invention adopts the following technical solution: a GABA hangover-relieving composition, the composition comprising the following components in parts by mass: 10-30 parts of γ-aminobutyric acid, 30-50 parts of Pueraria root extract, 20-30 parts of Hovenia dulcis fruit extract, 0.5-1 part of vitamin B1, 0.5-1 part of vitamin B6, 2-5 parts of Prunus mume extract, 2-5 parts of Imperata cylindrica root extract, 2-5 parts of corn oligopeptides, 2-6 parts of taurine, 1-2 parts of baicalin, and 1-2 parts of isorhamnetin.

[0007] Furthermore, the preparation method of the capsule comprises the following steps:

[0008] (1) Prepare a mixture of Tween 80 and Span 80 as an emulsifier, with the total amount of the emulsifier being 2-3% of the total weight of the hangover-relieving composition. Slowly add the emulsifier to deionized water at 40-50°C, stirring at 500-800 rpm. Prepare a uniform emulsifier solution and set aside.

[0009] (2) adding γ-aminobutyric acid, kudzu root extract, Hovenia dulcis fruit extract, vitamin B1, vitamin B6, Prunus mume extract, Imperata cylindrica root extract, corn oligopeptide, taurine, baicalin, and isorhamnetin to the emulsifier solution of step (1) for emulsification to obtain an emulsified solution;

[0010] (3) Weigh appropriate amounts of gelatin and arabic respectively, stir and dissolve them in hot water at 50-60℃ until the solution becomes clear and transparent to obtain the wall material solution.

[0011] (4) The emulsified solution of step (2) is slowly added dropwise to the wall material solution of step (3), and mixed at 50-60°C and a stirring speed of 400-600 rpm for 15-35 minutes. After uniform dispersion, 1-1.5 mol / L acetic acid solution is slowly added dropwise to adjust the pH value of the system to 4.2-4.5 for 20-30 minutes. A coagulation reaction occurs, and gelatin and gum arabic undergo a complex coagulation reaction to form a microcapsule wall on the surface of the raw material droplet. The acetic acid addition process needs to be slow and continuous stirring to prevent the local pH value from being too low, resulting in uneven coagulation.

[0012] (5) After the coagulation reaction in step (4) is completed, the microcapsules are prepared by aging at 5-10°C for 40-60 minutes to further solidify the microcapsule wall and enhance the stability of the microcapsules.

[0013] (6) The microcapsules prepared in step (5) are centrifuged, washed, and freeze-dried in a vacuum.

[0014] Furthermore, the mass ratio of Tween 80 to Span 80 in step (1) is 3:1.

[0015] Furthermore, the emulsification temperature of step (2) is 50-60°C, the shear rate is 10000-12000 rpm, and the emulsification time is 8-12 min.

[0016] Furthermore, in the wall material solution of step (3), the mass fraction of the gelatin solution is 5% to 8%, and the mass fraction of the gum arabic solution is 3% to 6%.

[0017] Furthermore, the mass ratio of gelatin to gum arabic in step (3) is 1.5:1.

[0018] Furthermore, the stirring speed of step (3) is 300-500 rpm.

[0019] Furthermore, the volume ratio of the emulsified solution to the wall material solution in step (4) is (0.8~1):1.

[0020] Furthermore, the centrifugal speed in step (6) is 4000-6000 rpm for 15-20 min, and the washing is repeated 3-4 times, and the washing is repeated with deionized water multiple times to remove the residual wall material, emulsifier, and unencapsulated raw material impurities on the surface. The microcapsules are pre-frozen at -45-50°C for 3-4 hours, and then sublimation dried at a vacuum degree of 20-40 Pa for 12-16 hours to maximize the retention of the active ingredients and structural integrity of the raw materials in the microcapsules.

[0021] The present invention also provides the use of the above-mentioned GABA-containing hangover-relief composition in hangover-relief products.

[0022] In the above technical solution, GABA is one of the main ingredients. GABA can regulate the nervous system, relieve anxiety and tension after drinking, and promote the metabolism of ethanol. Pueraria root extract accelerates alcohol metabolism, reduces alcohol damage to the liver, and relieves headaches and dizziness. Hovenia dulcis extract increases the activity of liver alcohol dehydrogenase and promotes alcohol metabolism. Vitamin B1 maintains the normal function of the nervous system and promotes liver metabolism. Vitamin B6 participates in alcohol metabolism and reduces the adverse effects of alcohol on the nervous system. Black plum extract promotes thirst and relieves discomfort symptoms after drinking. Imperata root extract, clears away heat and dampness, relieves symptoms of discomfort after drinking; corn oligopeptides, activates alcohol dehydrogenase activity, inhibits alcohol poisoning; taurine, strengthens the liver and promotes bile secretion, relieves fever and inflammation; baicalin, is an effective antioxidant, its molecular structure contains phenolic hydroxyl groups, which can provide hydrogen atoms, react with free radicals, thereby scavenging free radicals, and regulate the body's antioxidant enzyme system, thereby reducing oxidative damage caused by alcohol; isorhamnetin, can reduce the impact of alcohol on the nervous system by regulating the level of neurotransmitters, it can also increase the content of GABA or enhance its function, play a sedative and anti-anxiety role, and relieve the symptoms of nervous system excitement caused by alcohol.

[0023] Compared with the prior art, the present invention has the following beneficial effects:

[0024] (1) The GABA hangover-relief composition provided by the present invention is compounded with 11 ingredients, which can accelerate the decomposition of acetaldehyde in the liver, promote metabolism, protect the liver, and achieve excellent hangover-relief effects.

[0025] (2) The present invention prepares microcapsules by embedding the hangover-relieving composition containing GABA. The hangover-relieving effect of the microcapsules of the GABA hangover-relieving composition is more significant. Because GABA and other substances are easily affected by acid-base environments, unencapsulated GABA may undergo chemical reactions, such as decarboxylation reactions, resulting in loss of activity in the acidic environment of the gastrointestinal tract (pH 1.5-3.5) or alkaline digestive fluid (pH 7.5-9.0). Microcapsules can buffer the direct effects of the acid-base environment on the core material to a certain extent. When the microcapsules enter the gastrointestinal tract, the wall material slowly dissolves or swells, gradually releasing the core material, so that GABA and other components contact the digestive fluid in a relatively mild local environment, reducing the probability of chemical reactions. DETAILED DESCRIPTION

[0026] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0027] The reagents used in the present invention are all commercially available food grade, and unless otherwise specified, are conventional reagents, methods, and equipment in the art.

[0028] Example 1

[0029] An embodiment of the present invention provides a hangover-relief composition containing GABA, the composition comprising the following components in parts by mass: 20 parts of γ-aminobutyric acid, 40 parts of kudzu root extract, 25 parts of Hovenia dulcis fruit extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata cylindrica root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0030] The present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps:

[0031] (1) Prepare a mixture of Tween 80 and Span 80 in a mass ratio of 3:1 as an emulsifier. The total amount of the emulsifier is 2.5% of the total mass of the hangover detoxification composition. Slowly add the emulsifier to 45°C deionized water while stirring at 650 rpm. Prepare a uniform emulsifier solution and set aside.

[0032] (2) The above-mentioned GABA hangover-relieving composition was added to the emulsifier solution of step (1) according to the formula amount, and emulsified at a temperature of 55°C, a shear rate of 11,000 rpm, and a time of 10 min to obtain an emulsified solution.

[0033] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 6% and the mass fraction of gum arabic solution is 4%. Dissolve them in hot water at 55°C at a stirring speed of 400 rpm until the solution becomes clear and transparent to obtain a wall material solution.

[0034] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.9:1. The mixture was mixed at 55°C and a stirring speed of 500 rpm for 25 minutes. After uniform dispersion, 1.2 mol / L acetic acid solution was slowly added dropwise for 25 minutes to adjust the pH value of the system to 4.3.

[0035] (5) After the coagulation reaction in step (4) is completed, the microcapsules are prepared by aging at 8°C for 50 min.

[0036] (6) The microcapsules prepared in step (5) were centrifuged at 5000 rpm for 18 min, washed four times with deionized water, pre-frozen at -48°C for 3.5 h, and then sublimated and dried at a vacuum of 3 Pa for 14 h.

[0037] Example 2

[0038] An embodiment of the present invention provides a hangover-relief composition containing GABA, which is composed of the following components in parts by mass: 10 parts of γ-aminobutyric acid, 30 parts of kudzu root extract, 20 parts of Hovenia dulcis fruit extract, 0.5 parts of vitamin B1, 0.5 parts of vitamin B6, 2 parts of black plum extract, 2 parts of Imperata cylindrica root extract, 2 parts of corn oligopeptides, 2 parts of taurine, 1 part of baicalin, and 1 part of isorhamnetin.

[0039] The present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps:

[0040] (1) Prepare a mixture of Tween 80 and Span 80 in a mass ratio of 3:1 as an emulsifier. The total amount of emulsifier is 2% of the total mass of the hangover detoxification composition. Slowly add the emulsifier to 40°C deionized water while stirring at 500 rpm. Prepare a uniform emulsifier solution and set aside.

[0041] (2) The above-mentioned GABA hangover-relieving composition was added to the emulsifier solution of step (1) according to the formula amount, and emulsified at a temperature of 50°C, a shear rate of 10,000 rpm, and a time of 8 minutes to obtain an emulsified solution.

[0042] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 5% and the mass fraction of gum arabic solution is 3%. Dissolve them in hot water at 50°C at a stirring speed of 300 rpm until the solution becomes clear and transparent to obtain a wall material solution.

[0043] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.8):1, and the mixture was mixed at 50°C and a stirring speed of 400 rpm for 15 minutes. After uniform dispersion, 1 mol / L acetic acid solution was slowly added dropwise for 20 minutes to adjust the pH value of the system to 4.2.

[0044] (5) After the coagulation reaction in step (4) is completed, the mixture is aged at 5°C for 40 minutes to obtain microcapsules.

[0045] (6) The microcapsules prepared in step (5) were centrifuged at 4000 rpm for 20 min, washed four times with deionized water, pre-frozen at -45 °C for 3 h, and then sublimated and dried at a vacuum of 20 Pa for 16 h.

[0046] Example 3

[0047] An embodiment of the present invention provides a hangover-relief composition containing GABA, the composition comprising the following components in parts by mass: 30 parts of γ-aminobutyric acid, 50 parts of kudzu root extract, 30 parts of Hovenia dulcis fruit extract, 1 part of vitamin B1, 1 part of vitamin B6, 5 parts of black plum extract, 5 parts of Imperata cylindrica root extract, 5 parts of corn oligopeptides, 6 parts of taurine, 2 parts of baicalin, and 2 parts of isorhamnetin.

[0048] The present invention provides a method for preparing a capsule containing a GABA hangover-relieving composition, comprising the following steps:

[0049] (1) Prepare a mixture of Tween 80 and Span 80 in a mass ratio of 3:1 as an emulsifier. The total amount of the emulsifier is 3% of the total mass of the hangover detoxification composition. Slowly add the emulsifier to 50°C deionized water while stirring at 800 rpm. Prepare a uniform emulsifier solution and set aside.

[0050] (2) The above-mentioned GABA hangover-relieving composition was added to the emulsifier solution of step (1) according to the formula amount, and emulsified at a temperature of 60°C, a shear rate of 12,000 rpm, and a time of 12 minutes to obtain an emulsified solution.

[0051] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 8% and the mass fraction of gum arabic solution is 6%. Dissolve them in hot water at 60°C with a stirring speed of 500 rpm until the solution becomes clear and transparent to obtain a wall material solution.

[0052] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 1:1. The mixture was mixed at 60°C and a stirring speed of 600 rpm for 35 minutes. After uniform dispersion, 1.5 mol / L acetic acid solution was slowly added dropwise for 30 minutes to adjust the pH value of the system to 4.5.

[0053] (5) After the coagulation reaction in step (4) is completed, the mixture is aged at 10°C for 60 min to obtain microcapsules.

[0054] (6) The microcapsules from step (5) were centrifuged at 6000 rpm for 15 min, washed four times with deionized water, pre-frozen at -50 °C for 3 h, and then sublimated and dried at a vacuum of 40 Pa for 12 h.

[0055] Comparative Example 1

[0056] An embodiment of the present invention provides a hangover-relieving composition, which is composed of the following components in parts by mass: 40 parts of kudzu root extract, 25 parts of hovenia dulcis fruit extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata cylindrica root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0057] The present invention provides a method for preparing a capsule of a hangover-relieving composition, comprising the following steps:

[0058] (1) Prepare a mixture of Tween 80 and Span 80 in a mass ratio of 3:1 as an emulsifier. The total amount of the emulsifier is 2.5% of the total mass of the hangover detoxification composition. Slowly add the emulsifier to 45°C deionized water while stirring at 650 rpm. Prepare a uniform emulsifier solution and set aside.

[0059] (2) The above-mentioned hangover-relieving composition was added to the emulsifier solution of step (1) according to the formula amount, and emulsified at a temperature of 55°C, a shear rate of 11,000 rpm, and a time of 10 min to obtain an emulsified solution.

[0060] (3) Weigh gelatin and gum arabic in a mass ratio of 1.5:1, where the mass fraction of gelatin solution is 6% and the mass fraction of gum arabic solution is 4%. Dissolve them in hot water at 55°C at a stirring speed of 400 rpm until the solution becomes clear and transparent to obtain a wall material solution.

[0061] (4) The emulsified solution of step (2) was slowly added dropwise to the wall material solution of step (3), wherein the volume ratio of the emulsified solution to the wall material solution was 0.9:1. The mixture was mixed at 55°C and a stirring speed of 500 rpm for 25 minutes. After uniform dispersion, 1.2 mol / L acetic acid solution was slowly added dropwise for 25 minutes to adjust the pH value of the system to 4.3.

[0062] (5) After the coagulation reaction in step (4) is completed, the microcapsules are prepared by aging at 8°C for 50 min.

[0063] (6) The microcapsules prepared in step (5) were centrifuged at 5000 rpm for 18 min, washed four times with deionized water, pre-frozen at -48°C for 3.5 h, and then sublimated and dried at a vacuum of 3 Pa for 14 h.

[0064] Comparative Example 2

[0065] An embodiment of the present invention provides a hangover-relief composition containing GABA, the composition comprising the following components in parts by mass: 20 parts of γ-aminobutyric acid, 40 parts of kudzu root extract, 25 parts of Hovenia dulcis fruit extract, 0.7 parts of vitamin B1, 0.7 parts of vitamin B6, 3.5 parts of black plum extract, 3.5 parts of Imperata cylindrica root extract, 4 parts of corn oligopeptides, 4 parts of taurine, 1.5 parts of baicalin, and 1.5 parts of isorhamnetin.

[0066] The present invention provides a method for preparing a GABA hangover-relief composition tablet. The raw materials are mixed uniformly and then placed in a rotary tablet press for tableting. The pressure range is 12 kN and the tableting speed is 28 tablets / min.

[0067] Experimental example

[0068] Animal hangover experiment: 50 ICR mice were divided into five groups, namely a blank control group (administered with pure water), a model group (administered with liquor at a dose of 0.15 ml / 10 g), and an experimental group (the experimental group was Example 1 and Comparative Examples 1-2, each group was administered with liquor at a dose of 0.15 ml / 10 g, and the combination of each group was administered at a dose of 0.5 g / kg. The loss of the righting reflex (drunkenness) in mice was used as the successful drunkenness model. The drunkenness time and sobering time of the mice were observed and recorded. The results are shown in Table 1 below.

[0069] Table 1 The drunkenness time and soberness time of mice

[0070]

[0071] As shown in Table 1, the experimental group of Example 1 showed a longer duration of intoxication than the control groups 1-2, while the sobering time was shorter than that of the model group, demonstrating that the microcapsules containing the GABA hangover-relief composition prepared by the present invention have a significant hangover-relief effect. During this experiment, the blank control mice remained normal, while the model group mice became intoxicated after being administered alcohol, indicating that the intoxication model was successfully established.

[0072] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A hangover-relieving composition comprising GABA, characterized in that: The composition is composed of the following components in parts by mass: 10-30 parts of gamma-aminobutyric acid, 30-50 parts of kudzu root extract, 20-30 parts of Hovenia dulcis fruit extract, 0.5-1 part of vitamin B1, 0.5-1 part of vitamin B6, 2-5 parts of black plum extract, 2-5 parts of Imperata cylindrica root extract, 2-5 parts of corn oligopeptides, 2-6 parts of taurine, 1-2 parts of baicalin, and 1-2 parts of isorhamnetin; The preparation method thereof comprises the following steps: (1) Tween 80 and Span 80 are compounded as emulsifiers, with the total amount of emulsifier being 2-3% of the total mass of the hangover-relieving composition. The emulsifier is slowly added to deionized water at 40-50°C, while stirring at a speed of 500-800 rpm for 10-20 minutes to prepare a uniform emulsifier solution; (2) adding γ-aminobutyric acid, kudzu root extract, Hovenia dulcis fruit extract, vitamin B1, vitamin B6, Prunus mume extract, Imperata cylindrica root extract, corn oligopeptide, taurine, baicalin, and isorhamnetin to the emulsifier solution of step (1), emulsifying and shearing to obtain an emulsified solution; (3) Stir gelatin and arabic in hot water at 50-60°C until the solution becomes clear and transparent to obtain a wall material solution; (4) The emulsified solution of step (2) is slowly added dropwise to the wall material solution of step (3), and mixed at 50-60°C and a stirring speed of 400-600 rpm for 15-35 minutes. After uniform dispersion, 1-1.5 mol / L acetic acid solution is slowly added dropwise, and the pH value is adjusted to 4.2-4.5 for 20-30 minutes to allow a coagulation reaction to occur; (5) After the coagulation reaction in step (4) is completed, the mixture is aged at 5-10°C for 40-60 minutes to obtain microcapsules; (6) The microcapsules prepared in step (5) are centrifuged, washed, and freeze-dried in a vacuum.

2. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The mass ratio of Tween 80 to Span 80 in step (1) is 3:

1.

3. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The emulsification temperature of step (2) is 50-60°C, the shear rate is 10000-12000 rpm, and the emulsification time is 8-12 min.

4. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The mass fraction of the gelatin solution in the wall material solution of step (3) is 5% to 8%, and the mass fraction of the gum arabic solution is 3% to 6%.

5. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The mass ratio of gelatin to gum arabic in step (3) is 1.5:1; the stirring speed in step (3) is 300-500 rpm.

6. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The volume ratio of the emulsified solution to the wall material solution in step (4) is (0.8-1):

1.

7. The hangover-relieving composition containing GABA according to claim 1, characterized in that: The centrifugal speed of step (6) is 4000-6000 rpm, the time is 15-20 min, the washing is repeated 3-4 times, and the sample is pre-frozen at -50 to -45°C for 3-4 hours, and then dried at a vacuum degree of 20-40 Pa for 12-16 hours.

8. Use of the GABA-containing hangover-relief composition according to any one of claims 1 to 7 in the preparation of hangover-relief products.

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