A composition containing recombinant human albumin and acanthopanax senticosus extract

Abstract

The present invention relates to the field of cosmetics, and particularly relates to a composition containing recombinant human albumin and Eleutherococcus senticosus extract. The composition disclosed by the present invention includes 5.0 to 15.0 parts by mass of recombinant human albumin; 1.0 to 5.0 parts by mass of Eleutherococcus senticosus extract. In the composition of the present invention, the recombinant human albumin and the Eleutherococcus senticosus extract act synergistically, can effectively promote the proliferation of fibroblasts, increase skin elasticity, can effectively replenish water, make the skin moist and shiny, and effectively improve the conditions of skin pigmentation and wrinkles. The composition of the present invention has simple components and good biocompatibility, and can be widely applied to the preparation of anti-aging products.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and particularly to a composition containing recombinant human albumin and Acanthopanax senticosus extract. Background Art

[0002] The human skin is an organ that is prone to aging. As people age, the aging characteristics of the skin appear: more wrinkles, skin relaxation and yellowing, reduced luster, smoothness and gloss, coarser texture, increased pigmentation spots or depigmentation spots, etc. Currently, it is generally believed that the causes of skin aging mainly include two aspects: endogenous factors and exogenous factors. Endogenous factors include: (1) genetic factors, as the chromosomal telomere reaction sequence generated by genetic inheritance will change with age; (2) hormone deficiency, which leads to hormone aging due to the lack of hormones. For example, the lack of estrogen will reduce the function of the connective tissue of the skin and cause various skin changes. Exogenous factors include: (1) pollution factors, that is, the increasing waste gas and various pollutants in the atmosphere cause pollution to form a large number of free radicals. The attack of free radicals leads to the death and rapid aging of skin cells, such as skin asphyxia, pore blockage, and the skin becoming fragile; (2) photoaging, that is, the accumulation of UVA / UVB rays has some very harmful effects on the skin, such as disorder of the dermal tissue, thinning of the epidermal-dermal connective tissue that controls the fine adjustment of the skin, etc. In addition, excessive ultraviolet exposure is also the main cause of premature aging symptoms on the facial skin.

[0003] Anti-aging cosmetics are designed for the characteristics and formation reasons of aging skin. On the basis of moisturizing and sun protection, active nutrients are added to enhance the absorption of skin nutrients, and at the same time, the skin is further smoothed to achieve the purpose of delaying aging and removing wrinkles.

[0004] With the restricted use of many synthetic chemicals, "green" has become the development direction of cosmetics in the 21st century. Consumers' demand for safe and non-irritating cosmetics has promoted the application of natural active ingredients in cosmetics. Natural ingredients with physiological activities such as anti-free radical, antioxidant, anti-radiation, antibacterial, and anti-inflammatory are increasingly attracting people's attention. Currently, the development of natural active ingredients that can be applied to anti-aging cosmetics has become a trend.

[0005] Human serum albumin (HSA) is a monomeric protein produced by the liver. Mature human serum albumin contains 585 amino acid residues and has a molecular weight of 66KD. Albumin has abundant free sulfhydryl groups, has antioxidant properties, can scavenge free radicals (such as hypochlorous acid (HOCl) and peroxynitrite (ONOOH)) and provide sulfhydryl groups (-SH), thereby producing an anti-aging effect.

[0006] Acanthopanax senticosus (Rupr. et Maxim) Harms, a plant of the Araliaceae family, has dry roots, rhizomes or stems. It was recorded in "Shennong Ben Cao Jing" as early as in ancient times and was classified as a top-grade herb. It is mainly distributed in Korea, Japan, Russia and the northeastern region of China. Its nature and flavor are pungent, slightly bitter, and warm. It belongs to the spleen meridian, kidney meridian and heart meridian, and has the effects of replenishing qi and strengthening the spleen, tonifying the kidney and calming the mind. Acanthopanax senticosus is rich in flavonoids, saponins and other compounds. Modern pharmacological research shows that it has significant effects in aspects such as antioxidant, anti-inflammatory and improving immunity, and has extremely high economic and medicinal value.

[0007] Existing cosmetics use chemical reagents or plant extracts as the main components. Chemical reagents have poor biocompatibility and are prone to problems such as inflammation, swelling, allergy, pain, etc. The content of active ingredients in plant extracts is low and fluctuates due to the extraction process, resulting in a high dosage of plant extracts and increased costs. Therefore, existing cosmetics are difficult to simultaneously meet biocompatibility and antioxidant effects, and are difficult to meet the skin's antioxidant needs. Summary of the Invention

[0008] In view of the deficiencies of the prior art, the first aspect of the present invention provides a recombinant human albumin anti-aging composition, which includes: recombinant human albumin and Acanthopanax senticosus extract, wherein the mass fraction of recombinant human albumin is 5.0 - 15.0 parts; the mass fraction of Acanthopanax senticosus extract is 1.0 - 5.0 parts.

[0009] Furthermore, the composition further includes additives acceptable in the field of cosmetics.

[0010] Furthermore, the composition further includes at least one of a humectant, an emollient, a film-forming agent, an antioxidant, a whitening agent, a preservative, a fragrance, an emulsifier, a thickener, a stabilizer.

[0011] Further, the humectant includes at least one of glycerin, hydrogenated lecithin, sodium hyaluronate, ceramide, squalane, betaine, allantoin, butylene glycol, and polyethylene glycol; the emollient includes at least one of polydimethylsiloxane, isopropyl palmitate, isopropyl myristate, jojoba oil, olive oil, seaweed extract, and palmitoyl pentapeptide-3; the film-forming agent includes at least one of polydimethylsiloxane, polyvinyl alcohol, hydroxyethyl cellulose, chitosan, and sodium alginate; the antioxidant includes at least one of vitamin E, green tea extract, coenzyme Q10, ferulic acid, astaxanthin, vitamin C derivative, and resveratrol; the whitening agent includes at least one of niacinamide, glabridin extract, vitamin C derivative, tranexamic acid, arbutin, azelaic acid, and kojic acid; the preservative includes at least one of potassium sorbate, ethylhexylglycerin, phenoxyethanol, sodium dehydroacetate, chlorphenesin, and sodium benzoate; the fragrance includes at least one of phenethyl alcohol, linalool, and limonene; the emulsifier includes at least one of hydrogenated lecithin, span series, glyceryl stearate, cetearyl alcohol, polysorbate series, PEG-40 hydrogenated castor oil, and laureth; the thickener includes at least one of xanthan gum, hydroxyethyl cellulose, tragacanth gum, gum arabic, magnesium aluminum silicate, carbomer, and acrylic acid series; the stabilizer includes at least one of gum arabic, xanthan gum, cellulose derivative, vitamin E derivative, rosemary extract, green tea polyphenol, and astaxanthin.

[0012] Further, the dosage form of the composition is selected from at least one of cream, ointment, water, milk, essence, gel, oil, spray, powder, and mask.

[0013] It should be noted that the present invention does not strictly limit the components and mass fractions of acceptable additives in the field of cosmetics. All one or more additives having the functions of humectant, emollient, film-forming agent, antioxidant, whitening agent, preservative, fragrance, emulsifier, thickener, and stabilizer that are harmless to the human body selected according to the dosage form of the product or the conventional adjustment of the mass fractions of the additives according to actual needs are within the protection scope of the present invention.

[0014] In the second aspect of the present invention, there is provided the application of the composition in at least one of the following aspects:

[0015] A1: Application in the preparation of a product for increasing the viability of fibroblasts;

[0016] A2: Application in the preparation of a product for increasing skin activity;

[0017] A3: Application in the preparation of a product for increasing skin gloss;

[0018] A4: Application in the preparation of a product for supplementing skin moisture;

[0019] A5: Use in the preparation of products for lightening age spots;

[0020] A6: Use in the preparation of products for lightening fine lines;

[0021] A7: Use in the preparation of anti-aging products.

[0022] Furthermore, the product includes cosmetics. Further, the product includes facial cosmetics. Preferably, the facial cosmetics include at least one of cream, essence, sunscreen, and moisturizing gel.

[0023] In the third aspect of the present invention, a recombinant human albumin anti-aging cream is provided. The anti-aging cream includes: the mass fraction of recombinant human albumin is 5.0 - 15.0 parts; the mass fraction of Acanthopanax senticosus extract is 1.0 - 5.0 parts; the mass fraction of polydimethylsiloxane is 2.0 - 12.0 parts; the mass fraction of isopropyl palmitate is 0.5 - 10.0 parts; the mass fraction of hydrogenated lecithin is 1.5 - 8.0 parts; the mass fraction of glycerol is 2.0 - 8.0 parts; the mass fraction of tragacanth gum is 0.05 - 0.25 parts; the mass fraction of gum arabic is 0.05 - 0.30 parts; the mass fraction of polysorbate alcohol is 0.01 - 0.20 parts; the mass fraction of carbomer is 0.05 - 0.40 parts; the mass fraction of chlorphenesin is 0.01 - 0.5 parts.

[0024] Furthermore, in the anti-aging cream, polydimethylsiloxane belongs to a film-forming agent and an emollient; isopropyl palmitate belongs to an emollient; hydrogenated lecithin belongs to a moisturizer and an emulsifier; glycerol belongs to a moisturizer; tragacanth gum belongs to a thickener and a stabilizer; gum arabic belongs to a thickener and a stabilizer; carbomer belongs to a high molecular weight thickener; polysorbate alcohol belongs to an emulsifier; chlorphenesin belongs to a preservative.

[0025] In the fourth aspect of the present invention, a method for preparing the composition is provided. The method includes the following steps:

[0026] S1: Preparation of Acanthopanax senticosus extract: Crush Acanthopanax senticosus, pass through a 10-mesh sieve to obtain powder, soak it with 70% - 85% (v / v) ethanol according to the mass ratio of material to liquid of 1:(3 - 8), reflux extract at a water bath temperature of 60 - 100°C, collect the filtrate, distill the filtrate to remove ethanol until there is no alcohol smell, dissolve the Acanthopanax senticosus extract with deionized water and mix evenly, and spray-dry the mixed solution to obtain the Acanthopanax senticosus extract;

[0027] S2: Dissolve the acanthopanax senticosus extract in the recombinant human albumin aqueous solution or recombinant human albumin salt buffer solution, and use a high-pressure homogenizer to homogenize it at 500 - 1500 bar for 25 - 35 min at a temperature of 25 - 50 °C to obtain a uniform composition solution. Preferably, the recombinant human albumin salt buffer solution includes at least one of recombinant human albumin phosphate buffer solution, recombinant human albumin citrate buffer solution, and recombinant human albumin acetate buffer solution. Preferably, the recombinant human albumin buffer salt solution is recombinant human albumin PBS buffer solution.

[0028] Further, the conditions for spray drying in step S1 are: inlet air temperature for spray drying: 120 - 160 °C, atomization pressure: 0.1 - 1.0 Mpa, feeding rate: 400 - 800 ml / h.

[0029] Further, the ethanol concentration in step S1 is 70%, 73%, 75%, 78%, 80%, 83%, 85% or any value therebetween; the mass ratio of acanthopanax senticosus to ethanol is 1:3, 1:3.5, 1:4, 1:4.5, 1:5, 1:5.5, 1:6, 1:6.5, 1:7, 1:7.5, 1:8 or any value therebetween. Further, the time for soaking the acanthopanax senticosus extract in ethanol is 4 - 8 h.

[0030] Further, the extraction time for the water bath extraction step is 15 - 25 min; further, the water bath extraction step can be repeated 2 - 5 times. Further, the water bath temperature extraction step can be repeated 2, 3, 4, or 5 times. Preferably, this step is repeated 3 times. Further, it is necessary to combine the filtrates of the repeatedly extracted acanthopanax senticosus extracts and then distill.

[0031] Further, the method further includes a step of preparing a cosmetic by adding additives acceptable in the cosmetic field to the composition.

[0032] In a specific embodiment of the present invention, a method for preparing an anti-aging cream containing recombinant human albumin using the composition is provided. The method includes the following steps:

[0033] (1) The acanthopanax senticosus extract is prepared as follows: Crush the acanthopanax senticosus used in the formula and pass it through a 10-mesh sieve to obtain powder. Use 75% ethanol to soak it according to a solid-liquid ratio of 1:(3 - 8) for 4 - 8 h, reflux and extract it at a water bath temperature of 60 - 100 °C for 15 - 25 min, extract 3 - 5 times, combine the filtrates, distill off the ethanol until there is no alcohol smell, suspend the acanthopanax senticosus extract in deionized water, and spray dry the suspended solution to obtain the acanthopanax senticosus extract.

[0034] (2)Inlet air temperature for spray drying: 120~160 °C, atomization pressure: 0.1~1.0 Mpa, feeding speed: 400~800 ml / h;

[0035] (3)Hydrogenated lecithin, gum arabic, tragacanth gum, polydimethylsiloxane, isopropyl palmitate, and chlorophenylglycol are stirred at 40~80 rpm, temperature 75~105 °C, and time 15~50 min according to mass parts to obtain Phase A. Glycerol, polysorbate, and carbomer are stirred at 35~80 rpm, temperature 55~85 °C, and time 15~45 min according to mass parts to obtain Phase B. Phase A, Phase B, and the spray-dried powder of acanthopanax senticosus extract in mass parts are added to the recombinant human albumin aqueous solution or buffer solution in mass parts, and homogenized at 500~1500 bar for 30 min at a temperature of 25~50 °C using a high-pressure homogenizer to obtain a mixed solution;

[0036] (4)The mixed solution after high-pressure homogenization is placed in a refrigerator at -4 °C and left for 24 h to obtain the recombinant human albumin anti-aging cream.

[0037] The beneficial effects of the present invention include but are not limited to:

[0038] 1. In the composition of the present invention, recombinant human albumin and acanthopanax senticosus extract act synergistically, can effectively promote the proliferation of fibroblasts, increase skin elasticity, can effectively moisturize the skin, make the skin moist and shiny, and effectively improve the conditions of skin pigmentation and wrinkles. The composition of the present invention has simple components and good biocompatibility, and can be widely used in the preparation of anti-aging skin care products;

[0039] 2. The preparation method of the composition of the present invention has a simple manufacturing process and high application prospects. Detailed Embodiments

[0040] The present invention will be described in detail below with reference to the embodiments. However, the present invention is not limited to these embodiments. Unless otherwise specified, the raw materials and catalysts in the embodiments of the present invention are purchased through commercial channels.

[0041] Definition: Recombinant human albumin refers to albumin prepared using recombinant DNA technology for treatment and prevention.

[0042] Material source: Recombinant human albumin: Tonghua Anruite Biopharmaceutical Co., Ltd., batch number: ART101S;

[0043] Acanthopanax senticosus is purchased from Kangmei Xinkaihe (Jilin) Pharmaceutical Co., Ltd., batch number: 20231120;

[0044] Equipment:

[0045] Water bath (HH.S21-8, Shanghai Boxun Medical Biological Instrument Co., Ltd.);

[0046] Multifunctional fluorescence microplate reader (SPMax 3500FL type, Shanghai Flash Spectrum Biotechnology Co., Ltd.);

[0047] Analytical balance (BSA224S type, Sartorius Scientific Instruments Beijing Co., Ltd.);

[0048] Human fibroblasts (Lot number: CBP61613, Nanjing Kebai Biotechnology Co., Ltd.);

[0049] pH meter (PB-10 type, Sartorius Scientific Instruments Beijing Co., Ltd.);

[0050] Facial image analysis VISIA image analysis software (Canfied Company, USA);

[0051] Skin elasticity test probe Cutometer (MPA580, CK Company, Germany);

[0052] Skin microscope and active skin surface analysis system VisioScan (VC20plus, CK Company, Germany);

[0053] Other reagents and instruments used are all commercially available, unless otherwise specified.

[0054] Example 1 Preparation of recombinant human albumin-containing anti-aging composition The composition contains the following components in parts by mass: The active ingredients are shown in Table 1.

[0055] Table 1 Composition table of various recombinant human albumin-containing anti-aging compositions

[0056]

[0057] The specific steps for preparing a recombinant human albumin-containing anti-aging composition are as follows:

[0058] (1) The acanthopanax senticosus extract is prepared by the following method: The acanthopanax senticosus used in the formula is crushed and passed through a 10-mesh sieve to obtain powder. It is soaked with 75% ethanol at a solid-liquid ratio of 1:5 for 6 h, refluxed and extracted at a water bath temperature for 15 min, extracted 3 times, the filtrates are combined, and ethanol is distilled off until there is no alcohol smell. The acanthopanax senticosus extract is suspended in purified water, the above solution is mixed evenly, and the mixed solution is spray-dried to obtain the acanthopanax senticosus extract;

[0059] (2) Inlet air temperature for spray drying: 120~160 °C, preferably 150 °C; atomization pressure: 0.1~1.0 Mpa, preferably 0.5 Mpa; feeding speed: 200~800 ml / h, preferably 450 ml / h;

[0060] (3) Spray-dried powder of the Acanthopanax senticosus extract in parts by mass was added to the aqueous solution of recombinant human albumin in parts by mass, and high-pressure homogenization was carried out using a high-pressure homogenizer at 500 - 1500 bar for 30 min at a temperature of 25 - 50 °C to obtain a mixed solution.

[0061] Test Example 1 Cell Activity Test

[0062] On the basis of referring to the standard of T / ZHCA021 - 2022, this experiment was correspondingly optimized using the CCK-8 method. The effects of an anti-aging composition of the present invention (groups 1 - 11) and a blank control group (group 12) on the viability of human fibroblasts were determined: Cells in the logarithmic growth phase in good condition were inoculated into a 96-well plate at a density of 5×10 3 cells / 100 μl and incubated overnight in a constant temperature incubator at 37 °C and 5% CO2. Cells were treated with groups (1 - 12) respectively and cultured for 24 h. 10 μl of CCK-8 reagent was added to each well and incubated at 37 °C for another 1 h, and the OD 450nm value was measured to calculate the cell viability. Among them, the OD 450nm value of the experimental group was denoted as A s , and the OD 450nm values of the control group and the blank group were denoted as A c and A b , and all samples were measured three times. The cell activity was calculated according to the following formula, and the experimental data are shown in Table 2.

[0063] Cell viability (%) = [((A s - A b ) / (A c - A b ))] × 100%

[0064] A s : Absorbance of the experimental group (containing cells, culture medium, CCK-8 solution and anti-aging composition);

[0065] A c : Absorbance of the control group (containing cells, culture medium, CCK-8 solution, without anti-aging composition);

[0066] A b : Absorbance of the blank group (containing culture medium, CCK-8 solution, without cells, anti-aging composition).

[0067] Table 2 Experimental Results of Cell Activity Test

[0068]

[0069] Note: Compared with the blank control group, P ≤ 0.05, * indicates significant difference.

[0070] Effect of the anti-aging composition on fibroblast viability: The effect on fibroblast viability was investigated through the prepared groups (1 - 11). The results showed that the cell survival rate was above 102%, indicating that the prepared recombinant human albumin anti-aging composition was non-toxic to cells and that the recombinant human albumin anti-aging composition could effectively promote fibroblast proliferation.

[0071] Meanwhile, compared with the experimental groups (groups 1 - 5), the experimental data of the groups that did not add recombinant human albumin and Eleutherococcus senticosus extract simultaneously (groups 6 - 7) and the groups that added animal and plant albumin (groups 8 - 9) showed lower cell survival rates, indicating that the anti-aging composition of the present invention could maintain a high cell activity only when recombinant human albumin and Eleutherococcus senticosus extract coexisted. At the same time, compared with the blank control group, the cell activity value of the experimental groups was > 120%, and there was a significant difference between experimental group 3 and other groups, with the cell activity being 125.5 ± 0.16%.

[0072] Test Example 2 Human skin irritation test

[0073] According to the "Technical Specifications for Cosmetics Safety" (2015 edition), a human patch test was conducted. Twenty male and twenty female subjects aged 20 - 30 years old (without skin diseases and iatrogenic skin sensitivity) were selected. The patch test was carried out on the inner forearm and behind the ear of the subjects. For the patch test behind the ear, the test substance patch was removed 30 minutes after the test. For the patch test on the inner forearm, the test substance patch was removed at 0.5, 12, and 24 hours after the test. After the test, the skin reaction was observed and evaluated with reference to Table 3.

[0074] Table 3 Skin patch test evaluation criteria

[0075]

[0076] The experimental results showed that the skin patch test on the subjects showed that the patch test results of all 40 subjects were negative, indicating that the composition prepared by the invention patent had no obvious irritation and sensitization to the human body, that is, it had good safety performance.

[0077] Test Example 3 Skin anti-aging test

[0078] According to the product test requirements, the experimental research subjects were 210 healthy women aged 30 - 60 years old. Among them, there were 70 people aged 30 - 40 years old, 70 people aged 41 - 50 years old, and 70 people aged 51 - 60 years old. According to the "Technical Specifications for Cosmetics Safety" (2015 edition) standard, subjects were selected. According to the experimental requirements, those with obvious aging phenomena such as wrinkles and age spots on the face were selected. The grouping information is shown in Table 4.

[0079] Table 4 Grouping information for skin anti-aging test

[0080]

[0081] After cleansing the skin of the volunteer subjects, an appropriate amount of the anti-aging compositions prepared in Group 1, 3, 5, 6, 7, 10, and 11 of Example 1 was applied to the face, gently massaged until absorbed, and applied once in the morning and once in the evening every day for 30 consecutive days. It was tested once before use and once after 1 month, and the average value of the test results was taken. The elasticity, skin gloss, moisture content, number of spots (per cm 2 ), texture depth (μm), and wrinkle density (per cm 2 ), were used as the investigation indicators, and the anti-aging compositions prepared in Group 1, 3, 5, 6, 7, 10, and 11 of Example 1 were used for testing. The data results are shown in Tables 5 to 8.

[0082] Table 5 Test results of elasticity, brightness, and moisture content before use

[0083]

[0084] Table 6 Test results of the number of spots (per cm 2 ), texture depth (μm), and wrinkle density (per cm 2 ) before use

[0085]

[0086] Table 7 Test results of elasticity, brightness, and moisture content after use

[0087]

[0088] Table 8 Test results of the number of spots (per cm 2 ), texture depth (μm), and wrinkle density (per cm 2 ) after use

[0089]

[0090] As can be seen from the data in Tables 5 to 8, when using the anti-aging composition prepared by this invention patent, the average values of all scores have a certain increase. It shows that using this composition can improve skin elasticity, gloss, and increase skin water content in the short term, and effectively improve the conditions of skin pigmentation and wrinkles.

[0091] Example 2 Preparation of an anti-aging cream containing recombinant human albumin includes the following components in parts by mass: The active ingredients are shown in Table 9.

[0092] Table 9 Composition table of various anti-aging creams containing recombinant human albumin

[0093]

[0094] The specific steps for preparing an anti-aging cream containing recombinant human albumin are as follows:

[0095] (1) The acanthopanax senticosus extract is prepared by the following method: The acanthopanax senticosus used in the formula is crushed and passed through a 10-mesh sieve to obtain powder. It is soaked with 75% ethanol at a material-liquid ratio of 1:5 for 6 h, reflux-extracted at a water bath temperature for 15 min, extracted 3 times, the filtrates are combined, the ethanol is distilled off until there is no alcohol smell, the acanthopanax senticosus extract is suspended with purified water, the above solution is mixed evenly, and the mixed solution is spray-dried to obtain the acanthopanax senticosus extract;

[0096] (2) Inlet air temperature for spray drying: 120 - 160 °C, preferably 150 °C; atomization pressure: 0.1 - 1.0 Mpa, preferably 0.5 Mpa; feeding speed: 200 - 800 ml / h, preferably 450 ml / h;

[0097] (3) Hydrogenated lecithin, gum arabic, tragacanth gum, polydimethylsiloxane, isopropyl palmitate, and chlorphenesin are stirred at 40 - 80 rpm, at a temperature of 75 - 105 °C for 15 - 50 min according to the mass fraction to obtain phase A. Glycerol, polysorbate alcohol, and carbomer are stirred at 35 - 80 rpm, at a temperature of 55 - 85 °C for 15 - 45 min according to the mass fraction to obtain phase B. Phase A, phase B, and the spray-dried powder of the acanthopanax senticosus extract in terms of mass fraction are added to the recombinant human albumin in terms of mass fraction, and high-pressure homogenization is carried out at 500 - 1500 bar for 30 min at a temperature of 25 - 50 °C to obtain a mixed solution;

[0098] (4) The mixed solution after high-pressure homogenization is placed in a -4 °C refrigerator and left for 24 h to obtain the recombinant human albumin anti-aging cream.

[0099] Test Example 4 Cell Activity Test

[0100] The experimental steps of Test Example 1 are repeated by replacing the anti-aging cream prepared in Example 2 with the anti-aging composition in Test Example 1, and the experimental results are shown in Table 10.

[0101] Table 10 Experimental Results of Cell Activity Test

[0102]

[0103] Note: Compared with the blank control group, P ≤ 0.05, * indicates significant difference.

[0104] Effect of the anti-aging cream on the viability of fibroblasts: The cell viability in all experimental groups containing active ingredients (Groups 1-5, 8-9) was > 103% (vs. the blank control group). Among them, the recombinant human albumin group (Group 3) showed the best performance, with a cell viability of 135.7 ± 0.13% (p < 0.05), confirming that the cream formulation not only has no cytotoxicity but also has a significant proliferative effect on fibroblasts. The cell viability in the single-component deletion experimental groups (Groups 6-7, without adding recombinant human albumin and Acanthopanax senticosus extract simultaneously) decreased compared with that in the full-component groups (Groups 1-5), indicating that there is a synergistic mechanism between the two core active ingredients, and their combined use is a necessary condition for achieving high cell viability. The ratio experiment showed that (Groups 1-5), as the concentration of recombinant human albumin increased, the cell viability first increased and then stabilized, and the best effect concentration appeared in Group 3, indicating that the cell proliferation effect is the strongest under specific ratios.

[0105] Test Example 5 Determination Experiment of Hygienic Indexes and Heavy Metals

[0106] According to the microbial and heavy metal test methods in the "Technical Specifications for Cosmetic Safety" (2015 Edition), the total number of colonies, Escherichia coli thermotolerans, Pseudomonas aeruginosa, Staphylococcus aureus, molds, yeasts and heavy metals in a recombinant human albumin anti-aging cream (Groups 1-5) were determined. The microbiological index data are shown in Table 11, and the experimental results of heavy metal detection are shown in Table 12.

[0107] Table 11 Determination Results of Microbiological Indexes for Each Group

[0108]

[0109] As can be seen from Table 11, the test results of all indexes in the hygiene of the anti-aging cream (Groups: 1-5) meet the regulations.

[0110] Table 12 Determination Results of Heavy Metal Indexes for Each Group

[0111]

[0112] As can be seen from Table 12, the test results of the heavy metal detection indexes of the anti-aging cream (Groups: 1-5) meet the regulations.

[0113] Test Example 6 Human Skin Irritation Test

[0114] Replace the anti-aging cream prepared in Example 2 with the anti-aging composition in Test Example 2 and repeat the experimental steps of Test Example 2.

[0115] The experimental results showed that the patch test on the skin of the subjects showed that the patch test results of all 40 subjects were negative, indicating that the cream prepared by the invention patent has no obvious irritation and sensitization to the human body, that is, the safety performance is good.

[0116] Test Example 7 Skin Anti-aging Test

[0117] Repeat the experimental steps of Test Example 4 by replacing the anti-aging creams of Group 1, 3, and 5 prepared in Example 2 with the anti-aging compositions in Test Example 3. The grouping information is shown in Table 13. The experimental results are shown in Tables 14 to 17.

[0118] Table 13 Grouping Information for Skin Anti-aging Test

[0119]

[0120] Table 14 Test Results of Elasticity, Luminance, and Moisture Content Before Use

[0121]

[0122] Table 15 Test Results of Number of Spots (pcs / cm 2 ), Texture Depth (μm), and Wrinke Density (pcs / cm 2 ) Before Use

[0123]

[0124] Table 16 Test Results of Elasticity, Luminance, and Moisture Content After Use

[0125]

[0126] Table 17 Test Results of Number of Spots (pcs / cm 2 ), Texture Depth (μm), and Wrinke Density (pcs / cm 2 ) After Use

[0127]

[0128] It can be seen from the data in Tables 14 to 17 that for the anti-aging cream prepared by this invention patent, the average values of all scores have a certain increase. It shows that using this cream can improve skin elasticity, glossiness and increase skin moisture content in a short period, and effectively improve the conditions of skin pigmentation and wrinkles.

[0129] As described above, the above are only embodiments of the present invention. The protection scope of the present invention is not limited by these specific embodiments, but is determined by the claims of the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the technical idea and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A recombinant human albumin anti-aging composition, characterized in that, The composition comprises: recombinant human albumin and acanthopanax senticosus extract, wherein the mass fraction of recombinant human albumin is 5.0 - 15.0 parts; the mass fraction of acanthopanax senticosus extract is 1.0 - 5.0 parts, and the extraction steps of acanthopanax senticosus extract are as follows: pulverize acanthopanax senticosus, sieve through a 10-mesh sieve to obtain powder, soak with 70% - 85% (v / v) ethanol according to a material-liquid mass ratio of 1:(3 - 8), reflux and extract at a water bath temperature of 60 - 100°C, collect the filtrate, distill the filtrate to remove ethanol until there is no alcohol smell, dissolve the acanthopanax senticosus extract in deionized water and mix evenly, and spray-dry the mixed solution to obtain the acanthopanax senticosus extract.

2. The composition according to claim 1, characterized in that, The composition further comprises additives acceptable in the field of cosmetics.

3. The composition according to claim 1, characterized in that, The composition further comprises at least one of a humectant, an emollient, a film-forming agent, an antioxidant, a whitening agent, a preservative, a fragrance, an emulsifier, a thickener, and a stabilizer.

4. The composition according to claim 1, wherein The dosage form of the composition is selected from at least one of cream, ointment, water, milk, essence, gel, oil, spray, powder, and mask.

5. Use of the composition according to any one of claims 1 - 4 in the preparation of a product for increasing the viability of fibroblasts.

6. Use of the composition according to any one of claims 1 - 4 in at least one of the following aspects: A1: Use in the preparation of a product for increasing skin activity; A2: Use in the preparation of a product for increasing skin gloss; A3: Use in the preparation of a product for supplementing skin moisture; A4: Use in the preparation of a product for lightening age spots; A5: Use in the preparation of a product for lightening fine lines.

7. Use of the composition according to any one of claims 1 - 4 in the preparation of an anti-aging product.

8. The application according to any one of claims 5-7, characterized in that The product includes cosmetics.

9. A recombinant human albumin anti-aging cream, characterized in that, The anti-aging cream comprises: the composition according to claim 1; the mass fraction of polydimethylsiloxane is 2.0 - 12.0 parts; the mass fraction of isopropyl palmitate is 0.5 - 10.0 parts; the mass fraction of hydrogenated lecithin is 1.5 - 8.0 parts; the mass fraction of glycerol is 2.0 - 8.0 parts; the mass fraction of tragacanth gum is 0.05 - 0.25 parts; the mass fraction of gum arabic is 0.05 - 0.30 parts; the mass fraction of polysorbate is 0.01 - 0.20 parts; the mass fraction of carbomer is 0.05 - 0.40 parts; the mass fraction of chlorphenesin is 0.01 - 0.5 parts.

10. A method for preparing the composition according to any one of claims 1-4, characterized in that, The method comprises the following steps: S1: Prepare acanthopanax senticosus extract: pulverize acanthopanax senticosus, sieve through a 10-mesh sieve to obtain powder, soak with 70% - 85% (v / v) ethanol according to a material-liquid mass ratio of 1:(3 - 8), reflux and extract at a water bath temperature of 60 - 100°C, collect the filtrate, distill the filtrate to remove ethanol until there is no alcohol smell, dissolve the acanthopanax senticosus extract in deionized water and mix evenly, and spray-dry the mixed solution to obtain the acanthopanax senticosus extract; S2: Dissolve the mass fraction of acanthopanax senticosus extract in an aqueous solution of recombinant human albumin or a salt buffer, and use a high-pressure homogenizer to homogenize at 500 - 1500 bar for 25 - 35 min at a temperature of 25 - 50°C to obtain a uniform solution of the composition.

11. The method according to claim 10, wherein The conditions of the spray drying described in step S1 are as follows: inlet air temperature for spray drying: 120~160°C, atomization pressure: 0.1~1.0 Mpa, feeding rate: 400~800 mL / h.

12. The method according to claim 10, wherein The method further comprises the step of preparing a cosmetic by adding an additive acceptable in the field of cosmetics to the composition.

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