A firming and anti-wrinkle composition containing hydroxy pinacolone 9-cis retinoate and a preparation method thereof

Abstract

The present invention discloses a firming and anti-wrinkle composition containing hydroxypinacolone 9-cis-retinoate and a preparation method thereof, specifically belonging to the technical field of cosmetics; the firming and anti-wrinkle composition contains the following components in mass percentage: 0.03-0.3% hydroxypinacolone 9-cis-retinoate, 0.01-0.2% polydeoxyribonucleotide, 0.1-1% fructus corni extract, 2-5% emulsifier, 15-20% base oil, 0.5-5% humectant, 0.3-1% preservative, 0.1-1% thickener and deionized water up to 100%; the firming and anti-wrinkle composition containing hydroxypinacolone 9-cis-retinoate, polydeoxyribonucleotide and fructus corni extract provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the composition is mild and non-irritating and suitable for various people to use.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a firming and anti-wrinkle composition containing hydroxy-pinacolone 9-cis-retinoate and a preparation method thereof. Background Art

[0002] With the increasing demand for beauty and personal care products, the market demand for cosmetics and skin care products with firming and anti-wrinkle effects is also rising continuously. Traditional firming and anti-wrinkle products mostly rely on the supplement of collagen, the increase of moisturizing ingredients, or some physical means to improve skin firmness. However, these methods often have limited effects, and long-term use may have side effects or unstable effects.

[0003] In recent years, scientific research has found that some specific active ingredients have significant effects in improving skin aging signs, especially reducing wrinkles and enhancing skin firmness. Among them, it is recorded in CN119139160A that hydroxy-pinacolone 9-cis-retinoate has become an emerging ingredient in anti-wrinkle products due to its low irritation and good stability. It can promote skin cell renewal and enhance collagen synthesis, thus effectively improving skin laxity and wrinkle problems.

[0004] However, the use of a single ingredient often fails to achieve the ideal anti-wrinkle effect, and long-term dependence on a certain ingredient may also lead to reduced skin adaptability. Therefore, it is particularly important to develop a firming and anti-wrinkle composition with multi-component compounding and synergistic effects.

[0005] As a bioactive substance, polydeoxyribonucleotide has been found in recent years to have the effects of promoting skin cell regeneration and repairing damaged skin tissues. It can enhance the self-repair ability of the skin, thereby counteracting skin aging, while the extract of Cornus officinalis fruit is rich in various antioxidant components, which can scavenge free radicals, protect the skin from damage by the external environment, and at the same time has certain moisturizing and nourishing effects. Summary of the Invention

[0006] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a firming and anti-wrinkle composition containing hydroxy-pinacolone 9-cis-retinoate. By adding hydroxy-pinacolone 9-cis-retinoate, polydeoxyribonucleotide and the extract of Cornus officinalis fruit to the firming and anti-wrinkle composition, a more significant firming and anti-wrinkle effect can be achieved.

[0007] To achieve the above purpose, the present invention discloses the following technical solutions:

[0008] In the first aspect, the present invention provides a firming and anti-wrinkle composition. Calculated by mass percentage, the composition contains the following components:

[0009] Hydroxypinacolone 9-cis-retinoate 0.03 - 0.3%;

[0010] Polydeoxyribonucleotide 0.01 - 0.2%;

[0011] Cornus officinalis fruit extract 0.1 - 1%;

[0012] Emulsifier 2 - 5%;

[0013] Base oil 15 - 20%;

[0014] Humectant 0.5 - 5%

[0015] Preservative 0.3 - 1%;

[0016] Thickener 0.1 - 1%;

[0017] Deionized water to make up 100%.

[0018] Preferably, the preparation method of the Cornus officinalis fruit extract comprises the following steps:

[0019] Step 1-1. Select dry and pitted Cornus officinalis fruits, crush them to obtain Cornus officinalis fruit powder, and set aside;

[0020] Step 1-2. Prepare an ethanol aqueous solution with a concentration of 10 - 20 v / v%, adjust the pH to 4 - 5, and mix the Cornus officinalis fruit powder and the ethanol aqueous solution evenly at a solid-liquid ratio of 1:(8 - 15) g / mL to obtain a mixture to be extracted;

[0021] Step 1-3. Add a composite enzyme composed of pectinase and cellulase with a mass ratio of 1:(1 - 3) to the mixture to be extracted, wherein the pectinase enzyme activity is 15000 - 50000 U / g, the cellulase enzyme activity is 5000 - 20000 U / g, and the addition amount of the composite enzyme is 0.3 - 0.5 wt% of the mass of the Cornus officinalis fruit powder. After mixing evenly, first carry out enzymatic hydrolysis at 40 - 50 °C for 0.5 - 1.5 h, then carry out enzymatic hydrolysis at 50 - 60 °C for 0.5 - 1.5 h. After the gradient enzymatic hydrolysis is completed, heat up to 85 - 95 °C to inactivate the enzyme, and the enzyme inactivation time is ≥20 min. After the enzyme inactivation is completed, centrifuge and separate at 40 - 50 °C to obtain the supernatant, and then concentrate the supernatant to remove alcohol to obtain a crude extract;

[0022] Step 1-4. Pass the crude extract obtained in Step 1-3 through a polyamide chromatography column, elute with 1 BV - 2 BV of pure water, and then elute with 0.5 BV - 2.5 BV of an ethanol aqueous solution with a concentration of 50 - 80 v / v%, wherein the elution flow rate is 0.5 - 1 BV / h;

[0023] Step 1-5. Collect the ethanol aqueous solution eluate from Steps 1-4, and concentrate it under reduced pressure to obtain a Cornus officinalis fruit extract with a relative density of 1.025-1.125.

[0024] Preferably, the emulsifier is selected from at least one of PEG-100 stearate, glyceryl stearate, and polyglyceryl-3 methylglucose distearate.

[0025] Preferably, the base oil is selected from at least one of isononyl isononanoate, caprylic / capric triglyceride, and cetyl alcohol ethylhexanoate.

[0026] Preferably, the humectant is selected from at least one of 1,3-propanediol, butanediol, and glycerol.

[0027] Preferably, the thickener is selected from at least one of xanthan gum, polyacrylate crosslinked polymer-6, and sodium polyacryloyldimethyl taurate.

[0028] Preferably, the preservative is selected from at least one of phenoxyethanol, p-hydroxyacetophenone, and ethylhexylglycerin.

[0029] In a second aspect, the present invention provides a method for preparing the firming and anti-wrinkle composition described in the first aspect, and the preparation method includes the following steps:

[0030] Step 2-1. Heat and stir the emulsifier, base oil, and hydroxypinacolone retinoate 9-cis evenly to obtain Solution A;

[0031] Step 2-2. Heat and stir the thickener, preservative, humectant, and deionized water evenly to obtain Solution B;

[0032] Step 2-3. Add Solution A to Solution B and stir evenly to obtain Mixture C;

[0033] Step 2-4. Add polydeoxyribonucleotide and Cornus officinalis fruit extract to Mixture C and stir evenly to obtain the firming and anti-wrinkle composition.

[0034] Preferably, Step 2-1 is to heat and stir the emulsifier, base oil, and hydroxypinacolone retinoate 9-cis evenly at 80°C and 300 r / min to obtain Solution A.

[0035] Preferably, Step 2-2 is to heat and stir the thickener, preservative, humectant, and deionized water evenly at 80°C and 300 r / min to obtain Solution B.

[0036] Preferably, Step 2-3 is to add Solution A to Solution B at 80°C, homogenize at 7000 r / min for 5 min first, and then stir at 500 r / min for 20 min to obtain Mixture C.

[0037] Preferably, in Step 2-4, the mixture C is cooled to 45°C, and then polydeoxyribonucleotide and cornel fruit extract are added, followed by stirring at 300 r / min for 3 min to obtain the firming and anti-wrinkle composition.

[0038] In the present invention:

[0039] Hydroxypinacolone retinoate can resist fine lines and wrinkles, while promoting the production of collagen and elastin, improving the elasticity and firmness of the skin, making the skin smoother and more delicate. At the same time, it also has the effect of inhibiting tyrosinase, reducing pigment deposition, enhancing the gloss and firmness of the skin, making the skin color brighter. In addition, it has antioxidant properties, which can reduce the damage of free radicals to the skin, lower the skin roughness, and inhibit sebum secretion, making the skin more refreshing.

[0040] Polydeoxyribonucleotide can stimulate the activity of adenosine A2A receptor, affect transcription factors, and secrete growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF), thereby stimulating the growth of epidermal cells, increasing the synthesis of collagen, and promoting the repair and regeneration of damaged skin. At the same time, it has good anti-inflammatory properties and can reduce skin inflammatory reactions by inhibiting the expression of inflammatory cytokines.

[0041] The cornel fruit extract provided by the present invention is rich in morroniside, has excellent antioxidant performance, can effectively scavenge free radicals, resist oxidative stress reaction, delay skin aging, reduce wrinkles and relaxation. At the same time, it can inhibit the activity of inflammatory factors and alleviate skin redness and allergy.

[0042] Advantages of the present invention:

[0043] 1. The firming and anti-wrinkle composition containing hydroxypinacolone 9-cis-retinoate, polydeoxyribonucleotide and cornel fruit extract provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the composition is mild and non-irritating, suitable for various types of people to use.

[0044] 2. Hydroxypinacolone 9-cis-retinoate, polydeoxyribonucleotide and cornel fruit extract play a crucial role in firming and anti-wrinkle. Combining with the test results, it can be seen that these three components have a certain synergistic effect in firming and anti-wrinkle. Specific embodiments

[0045] To better illustrate the purpose, technical solution and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0046] Unless otherwise specified, the test methods used in the examples and comparative examples are all conventional methods; the materials, reagents, etc. used can be obtained from commercial sources unless otherwise specified; the percentages mentioned in the examples and comparative examples are all mass percentages unless otherwise specified.

[0047] In the present invention:

[0048] Hydroxypinacolone 9-cis-retinoate: Purchased from Hezhi Biotechnology (Nanjing) Co., Ltd.;

[0049] Polydeoxyribonucleotide: Purchased from Ruijiming (Shandong) Biotechnology Co., Ltd.;

[0050] Fructus Corni: The dried and mature sarcocarp of Cornus officinalis Sieb. et Zucc. ( Cornus officinalis ) was purchased from Guangdong Zhongke Zhuoyuan Biotechnology Co., Ltd.;

[0051] Pectinase: Purchased from Dongheng Huadao Biotechnology, with an enzyme activity of 15000 - 50000 U / g;

[0052] Cellulase: Purchased from Dongheng Huadao Biotechnology, with an enzyme activity of 5000 - 20000 U / g;

[0053] Polyamide chromatography column: Purchased from Merck & Co., Inc., with the model of Polyamide C66.

[0054] The remaining raw materials can be obtained commercially.

[0055] Preparation of Fructus Corni Extract

[0056] Step 1 - 1. Select dry and pitted Fructus Corni, place it in a high - speed pulverizer to pulverize it into powder, and set aside;

[0057] Step 1 - 2. Prepare an ethanol aqueous solution with a concentration of 10 - 20 v / v%, adjust the pH to 4 - 5, and mix the Fructus Corni powder and the ethanol aqueous solution evenly at a solid - liquid ratio of 1:(8 - 15) g / mL to obtain a mixture to be extracted;

[0058] Step 1 - 3. Add a complex enzyme composed of pectinase and cellulase with a mass ratio of 1:(1 - 3) to the mixture to be extracted. The addition amount of the complex enzyme is 0.3 - 0.5 wt% of the mass of Fructus Corni. After mixing evenly, keep it at 45 °C for 0.5 - 1.5 h first, then keep it at 55 °C for 0.5 - 1.5 h. After the gradient enzymatic hydrolysis is completed, raise the temperature to 85 - 95 °C to inactivate the enzyme, and the enzyme inactivation time is ≥20 min. After the enzyme inactivation is completed, centrifuge at 40 - 50 °C and 10000 rpm for 15 min, and concentrate the supernatant obtained by centrifugal separation to alcohol - free to obtain a crude extract;

[0059] Step 1-4. Pass the crude extract obtained in Step 1-3 through a polyamide chromatography column, elute with 1 BV - 2 BV of pure water, and then elute with 0.5 BV - 2.5 BV of an ethanol aqueous solution with a concentration of 50 - 80 v / v%, where the elution flow rate is 0.5 - 1 BV / h;

[0060] Step 1-5. Collect the ethanol aqueous solution eluate from Step 1-4 and concentrate it under reduced pressure to obtain a Cornus officinalis fruit extract with a relative density of 1.025 - 1.125.

[0061] Table 1 Preparation parameters of Cornus officinalis fruit extract

[0062]

[0063] Preparation of firming and anti-wrinkle essence milk

[0064] Accurately weigh each component according to the raw material mass percentages in Table 2;

[0065] Step 2-1. Stir the emulsifier, base oil, and hydroxy-pinacolone retinoate at 80 °C and 300 r / min until evenly mixed to obtain Solution A;

[0066] Step 2-2. Stir the thickener, preservative, humectant, and deionized water at 80 °C and 300 r / min until evenly mixed to obtain Solution B;

[0067] Step 2-3. At 80 °C, add Solution A to Solution B, homogenize at 7000 r / min for 5 min first, and then stir at 500 r / min for 20 min to obtain Mixture C;

[0068] Step 2-4. After cooling Mixture C to 45 °C, add polydeoxyribonucleotide and Cornus officinalis fruit extract, and stir at 300 r / min for 3 min to obtain the firming and anti-wrinkle essence milk.

[0069] Table 2 Components of the essence milk and their mass percentages

[0070]

[0071] Note: " / " in the table indicates no addition.

[0072] In order to verify the effects of the key components in the essence milk and the relationships between the key components, adjustments were made based on the formulation of Example 3, specifically as follows:

[0073] Table 3 Components of the comparative essence milk and their mass percentages

[0074]

[0075] Note: " / " in the table indicates no addition.

[0076] The preparation methods of Comparative Examples 1-5 are the same as that of Example 3, and the missing components can be not added in the corresponding steps.

[0077] Performance Test

[0078] 1 In vitro experiment

[0079] Antioxidant activity test

[0080] Samples to be tested: The essence milks prepared in Examples 1-4 and Comparative Examples 1-5.

[0081] The antioxidant activities of the samples to be tested were evaluated using the DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging method. This method is based on the principle of color change after the reaction between DPPH free radicals and antioxidants, and the antioxidant activity is quantified by measuring the change in absorbance.

[0082] DPPH solution: Dissolve 1 mg of DPPH in 24 mL of absolute ethanol to prepare a 0.1 mmol / L DPPH solution, sonicate for 5 min, and shake well to mix evenly.

[0083] Sample group to be tested: The samples to be tested were respectively prepared into sample solutions with a concentration of 50 wt% using absolute ethanol. Take 2 mL of the sample solution to be tested and 2 mL of the DPPH solution and add them to the same test tube, shake well, and let it stand in the dark at room temperature for 30 min, then measure the absorbance value at 520 nm, denoted as A 待测样品 ;

[0084] Control group: At the same time, measure the absorbance value after mixing 2 mL of the DPPH solution and 2 mL of absolute ethanol, denoted as A 对照 ;

[0085] Background group: The absorbance value measured after replacing the DPPH solution in the sample group to be tested with an equal volume of absolute ethanol is denoted as A 10 ; The absorbance value measured after replacing the DPPH solution in the control group with an equal volume of absolute ethanol is denoted as A 20 .

[0086] Calculate the scavenging ability of different samples to be tested for DPPH free radicals according to the following formula, and the results are shown in Table 4.

[0087] DPPH free radical scavenging rate = [1 - (A 待测样品 - A 10 ) / (A 对照 - A 20 )] × 100%

[0088] Elastase inhibition assay

[0089] Samples to be tested: The essence milks prepared in Examples 1-4 and Comparative Examples 1-5.

[0090] The in vitro elastase inhibition experiment was used to evaluate the effect of the samples on the activity of skin elastase. A specific fluorescent substrate was used to measure the reduction of enzyme activity, thereby evaluating the anti-wrinkle and firming effect.

[0091] Grouping was set according to the instructions of the kit:

[0092] Samples to be tested group: Add 50 μL of the sample solution to be tested with a concentration of 50 wt% prepared with the buffer in the kit to a black 96-well bottom transparent plate, then add 100 μL of elastase working solution at 0.2 U / mL. After shaking and reacting at room temperature for 15 min, add 50 μL of elastase fluorescence conjugate at 100 μg / mL to each well. After incubating in the dark at room temperature for 30 min, measure the fluorescence intensity at an excitation wavelength of 485 nm and an emission wavelength of 530 nm with a fluorescence microplate reader, denoted as MFI 待测样品 ;

[0093] Control group: Replace the sample solution to be tested with an equal volume of 50 μL of buffer, and keep the rest unchanged. The measured fluorescence intensity is denoted as MFI 对照 ;

[0094] Background group: Replace the sample solution to be tested with 50 μL of buffer, and replace the elastase working solution with 100 μL of buffer, and keep the rest unchanged. The measured fluorescence intensity is denoted as MFI 背景 .

[0095] Calculate the inhibitory ability of different samples to be tested on elastase according to the following formula, and the results are shown in Table 4.

[0096] Elastase inhibition rate = [1 - (MFI 待测样品 - MFI 背景 ) / (MFI 对照 - MFI 背景 )] × 100%

[0097] Table 4 Test results

[0098]

[0099] 2 Human trials

[0100] Take the essence milks prepared in Examples 1-4 and Comparative Examples 1-5 for human clinical trials.

[0101] 2.1 Safety test

[0102] The human skin closed patch test was carried out according to the human skin patch test specified in the Cosmetics Safety and Technology Standard.

[0103] 2.1.1 Test Purpose: To detect the potential possibility of the test substance causing adverse skin reactions in humans.

[0104] 2.1.2 Test Design: Apply the patch tester with the test substance to the back skin of the subject for 24 hours. Score the skin according to the skin reaction grading standard of the skin closed patch test at 30 minutes (after the indentation disappears), 24 hours, and 48 hours respectively after removing the patch tester with the test substance.

[0105] 2.1.3 Test Substance: The essence lotions prepared in Examples 1-4 and Comparative Examples 1-5.

[0106] 2.1.4 Test Instruments / Materials: Analytical balance, 1mm patch tester, medical adhesive tape, sterilized cotton swabs, and 1mL syringe;

[0107] 2.1.5 Test Method:

[0108] Volunteer Requirements: Select volunteers aged 18-60 who meet the test requirements as the test subjects, randomly divide them into groups, with 30 people in each group;

[0109] Test Procedure: Select a qualified patch test device with an area not exceeding 50mm 2 and a depth of about 1mm. Put the test substance into the small chamber of the patch tester, with the dosage about 0.020g - 0.025g. The control hole is a blank control without adding anything. Stick the patch tester with the test substance on the back of the subject with a low-allergy tape, and gently press it with the palm to make it evenly adhere to the skin for 24 hours.

[0110] Observe the skin reaction according to the standard in Table 5 at 30 minutes (after the indentation disappears), 24 hours, and 48 hours respectively after removing the patch tester with the test substance, and record the observation results. The results are shown in Table 6.

[0111] Table 5 Skin Reaction Grading Standard for Skin Closed Patch Test

[0112]

[0113] Table 6 Test Results of the Test Substance

[0114]

[0115] Result Analysis

[0116] The test results show that 0 out of 30 people had positive reactions. The essence lotions prepared in Examples 1-4 and Comparative Examples 1-5 provided by the present invention do not cause adverse skin reactions, are very mild, and have no irritation to human skin.

[0117] 2.2 Skin Elasticity Test

[0118] 2.2.1 Test Samples

[0119] The essence milks prepared in Examples 1-4 and Comparative Examples 1-5;

[0120] Essence milk matrices 1-4 are respectively based on the formulations of Examples 1-4 without adding hydroxypinacolone retinoate, polydeoxyribonucleotide and fructus corni extract, and the lack is supplemented with deionized water.

[0121] 2.2.2 Test Methods

[0122] 130 volunteers aged 30-45 with wrinkles and healthy and undamaged skin were selected and randomly divided into 13 groups, with 10 in each group, and the above test samples were used respectively. The subjects took 2 g of essence milk / essence milk matrix and applied it on the face, once in the morning and once in the evening every day for 30 consecutive days.

[0123] During the experiment, the subjects could not apply any other cosmetics on the experimental site. The skin elasticity meter Cutometer MPA580 was used to collect data at 0 day and 30th day. The test site was the zygomatic region. When measuring, the probe was repeatedly measured 3 times in the same measured area, and the average value was taken.

[0124] The test parameter was R2 (the ratio of the skin rebound amount Ua without negative pressure to the maximum stretching amount Uf with negative pressure). The closer R2 was to 1, the better the skin elasticity. The R2 change rate = (R2 value on the 30th day - R2 value on the 0th day) / R2 value on the 0th week.

[0125] 2.2.3 Test Results

[0126] As shown in Table 7.

[0127] Table 7 Skin Elasticity Test Results

[0128]

[0129] 2.3 Analysis of Test Results

[0130] According to the test results, the essence milk containing hydroxypinacolone retinoate, polydeoxyribonucleotide and fructus corni extract provided by the present invention has excellent firming and anti-wrinkle effects. At the same time, the essence milk is mild and non-irritating, and suitable for various people to use. The active ingredients hydroxypinacolone retinoate, polydeoxyribonucleotide and fructus corni extract in the essence milk play a crucial role in the overall formulation. At the same time, combined with the in vitro test results, it can be seen that these three ingredients, hydroxypinacolone retinoate, polydeoxyribonucleotide and fructus corni extract, have a certain synergistic effect in firming and anti-wrinkle.

[0131] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of this application claimed, but merely represents selected embodiments of this application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in this application without creative efforts shall fall within the scope of protection of this application.

Claims

1. A firming and anti-wrinkle composition, characterized in that, The composition contains the following components by mass percentage: Hydroxypinacolone 9-cis-retinoate 0.03 - 0.3%; Polydeoxyribonucleotide 0.01 - 0.2%; Cornus officinalis fruit extract 0.1 - 1%; Emulsifier 2 - 5%; Base oil 15 - 20%; Humectant 0.5 - 5%; Preservative 0.3 - 1%; Thickener 0.1 - 1%; Deionized water to make up 100%; The preparation method of the Cornus officinalis fruit extract includes the following steps: Step 1-1. Select dried and pitted Cornus officinalis fruit, crush it to obtain Cornus officinalis fruit powder, and set aside; Step 1-2. Prepare an ethanol aqueous solution with a concentration of 10 - 20 v / v%, adjust the pH to 4 - 5, and mix the Cornus officinalis fruit powder and the ethanol aqueous solution evenly at a material-liquid ratio of 1:(8 - 15) g / mL to obtain a mixture to be extracted; Step 1-3. Add a composite enzyme composed of pectinase and cellulase with a mass ratio of 1:(1 - 3) to the mixture to be extracted, where the pectinase enzyme activity is 15000 - 50000 U / g and the cellulase enzyme activity is 5000 - 20000 U / g. The addition amount of the composite enzyme is 0.3 - 0.5 wt% of the mass of the Cornus officinalis fruit powder. After mixing evenly, first carry out enzymatic hydrolysis at 40 - 50 °C for 0.5 - 1.5 h, then carry out enzymatic hydrolysis at 50 - 60 °C for 0.5 - 1.5 h. After the gradient enzymatic hydrolysis is completed, heat up to 85 - 95 °C to inactivate the enzyme, and the enzyme inactivation time is ≥20 min. After the enzyme inactivation is completed, centrifuge and separate at 40 - 50 °C to obtain the supernatant, and then concentrate the supernatant to be alcohol-free to obtain a crude extract; Step 1-4. Pass the crude extract obtained in Step 1-3 through a polyamide chromatography column, wash it with 1 BV - 2 BV of pure water, and then elute it with 0.5 BV - 2.5 BV of an ethanol aqueous solution with a concentration of 50 - 80 v / v%, where the elution flow rate is 0.5 - 1 BV / h; Step 1-5. Collect the ethanol aqueous solution eluate in Step 1-4, and concentrate it under reduced pressure to obtain a Cornus officinalis fruit extract with a relative density of 1.025 - 1.

125.

2. The firming and anti-wrinkle composition according to claim 1, wherein The emulsifier is selected from at least one of PEG-100 stearate, glyceryl stearate, and polyglyceryl-3 methylglucose distearate.

3. The anti-wrinkle and firming composition according to claim 1, wherein The base oil is selected from at least one of isononyl isononanoate, caprylic / capric triglyceride, and cetyl ethylhexanoate.

4. The compacting and anti-wrinkle composition according to claim 1, characterized in that, The humectant is selected from at least one of 1,3-propanediol, butanediol, and glycerol.

5. The compacting and anti-wrinkle composition according to claim 1, wherein The thickener is selected from at least one of xanthan gum, polyacrylate cross-linked polymer-6, and sodium polyacryloyldimethyl taurate.

6. The firming and anti-wrinkle composition according to claim 1, wherein The preservative is selected from at least one of phenoxyethanol, p-hydroxyacetophenone, and ethylhexylglycerin.

7. A method for preparing the firming and anti-wrinkle composition according to any one of claims 1-6, characterized in that, The preparation method includes the following steps: Step 2-1. Heat and stir the emulsifier, base oil, and hydroxypinacolone 9-cis-retinoate evenly to obtain Solution A; Step 2-2. Heat and stir the thickener, preservative, humectant, and deionized water evenly to obtain Solution B; Step 2-3. Add Solution A to Solution B and stir evenly to obtain Mixture C; Step 2-4. Add polydeoxyribonucleotide and cornel fruit extract to mixture C, and stir evenly to obtain the firming and anti-wrinkle composition.

8. The preparation method according to claim 7, wherein Step 2-1 is to stir the emulsifier, base oil, and hydroxypinacolone retinoate evenly at 80 °C and 300 r / min to obtain solution A.

9. The preparation method according to claim 7, characterized in that, Step 2-2 is to stir the thickener, preservative, humectant, and deionized water evenly at 80 °C and 300 r / min to obtain solution B.

10. The preparation method according to claim 7, characterized in that, Step 2-3 is to add solution A to solution B at 80 °C, homogenize at 7000 r / min for 5 min first, and then stir at 500 r / min for 20 min to obtain mixture C.

11. The preparation method according to claim 7, characterized in that, Step 2-4 is to cool mixture C to 45 °C, add polydeoxyribonucleotide and cornel fruit extract, and stir at 300 r / min for 3 min to obtain the firming and anti-wrinkle composition.

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