Anti-blue-light skin care composition containing anthriscus sylvestris extract and application of anti-blue-light skin care composition

Abstract

The invention relates to an anti-blue-light skin care composition containing anthriscus sylvestris extract and application thereof.The anti-blue-light skin care composition is suitable for Chinese people with skin types III and above, blue light can be effectively blocked, the phenomena of skin chromatosis, aging, sensitivity, dryness and the like caused by the blue light are reduced, and the skin care effect is good. And IL-6 rise caused by blue light irradiating skin tissues is inhibited, and skin itch caused by blue light is reduced.

Description

Technical Field

[0001] The present invention relates to the field of cosmetics, and in particular to an anti-blue light skin care composition comprising an extract of ginseng and its application Background Art

[0002] Photoaging is one of the main factors causing exogenous skin aging. Ultraviolet radiation is currently considered to be the main cause of photoaging. There are many studies on skin photoaging caused by ultraviolet rays, but there is not enough research on visible light, especially blue light.

[0003] Blue light is the light with the shortest wavelength (380-495nm) in the visible spectrum. It is a high-energy short-wave light. Blue light is divided into natural blue light and artificial blue light. Natural blue light comes from sunlight, while artificial blue light comes from various electronic devices in life. The white light emitted by light-emitting diodes (LEDs) is mainly produced by blue light chips stimulating yellow light phosphors, so there is an obvious peak in the LED spectrum with a higher color temperature. Electronic devices such as mobile phones, computers and televisions use light-emitting diodes (LEDs) to improve screen brightness and clarity, and LEDs are widely used in the field of lighting. However, visible light can reach the dermis of the skin and induce the production and expression of ROS, inflammatory cytokines and MMP1. Therefore, people are exposed to blue light sources (BL) for more than several hours every day in their lives. Blue light is an overlooked pathogenic factor of photoaging. Among the 40,000 skin care products launched in 2016, only 9 claimed to have blue light protection. Photoaging that may be caused by blue light has not received enough attention. Therefore, it is necessary to carry out research on anti-blue light cosmetics.

[0004] Solar radiation can be divided into infrared light, visible light and ultraviolet light, among which blue light is the shortest wavelength and highest energy band in the visible spectrum. Blue light can penetrate the skin and reach the subcutaneous tissue. The abundance of blue light in sunlight is more than twice that of UVA in UVR. Its penetrating ability is stronger than that of ultraviolet light, and it can also cause damage to human skin similar to that of ultraviolet light. Blue light mainly damages cells through photochemical effects. Long-term exposure to blue light can cause pigmentation, aging, sensitivity and dryness of the skin. For the Chinese population, the Fitzpatrick skin typing method divides the skin of the population into types Ι-VI, while the Chinese population is mainly type III and above. Studies have shown that visible light can produce more lasting pigmentation than UVA1 (340-400nm) radiation in people with skin types IV and above; further studies have found that blue light, rather than infrared light, causes obvious pigmentation in type III and IV subjects. Therefore, the Chinese population may be more likely to suffer from skin pigmentation when exposed to blue light.

[0005] In addition, Ning Jing reported that in clinical work, people who are exposed to a lot of light often experience skin itching. It has been reported that medical equipment containing blue light can induce a certain probability of skin itching reaction. In the gradient light experiment, two people experienced itching, and the irradiation dose was 75J / cm 2 ,95J / cm 2 According to the VAS score, all patients had mild pruritus. The Xie-Kawashima pruritus scale score was 2 points during the day and 0 points at night. The probability of pruritus was 6%. During the five-day light experiment, 9 patients had pruritus, of which 4 had a 75J / cm 2 ,5 people irradiated with a dose of 95J / cm 2 , the VAS score was 3-6 points, which was mild to moderate pruritus. In the Xie-Kawashima pruritus scale, the score was 2 points during the day and 0 points at night, and the probability of itching was 27%. RNA-seq analysis of Ha Ca T cells irradiated with blue light showed that there were 16 significantly down-regulated genes and 397 significantly up-regulated genes in the blue light group. Among the inflammatory factors related to skin barrier damage, IL-6 was significantly increased. The IL-6 content in mouse skin tissue was detected. The blue light group and TRPV4 activator group were significantly higher than the control group. Compared with the blue light group and solvent + blue light group, the IL-6 content in the TRPV4 antagonist + blue light group was significantly reduced, and there were statistical differences. Therefore, TRPV4 is related to the increase of IL-6 caused by blue light irradiation of skin tissue.

[0006] In addition, compared with UV, blue light has a longer wavelength and is more likely to penetrate the epidermis and dermis of the skin, causing serious damage to the mitochondria in epithelial cells; the light intensity emitted by blue light is close to the intensity of the sun's ultraviolet rays at noon, which can easily cause allergic phenomena such as redness, swelling, dryness, desquamation, and tightness on the skin; blue light can also change the structure of epidermal cells and reduce the production of collagen and elastin. The current sunscreens are mainly aimed at ultraviolet rays. From the perspective of light scattering, nano-scale inorganic sunscreens can pass through ultraviolet light and visible light; from the perspective of light absorption, nano-scale inorganic sunscreens can only effectively absorb ultraviolet rays, and the energy of visible light is not enough to excite the electrons in its valence band, so it cannot be absorbed. Therefore, nano-scale inorganic sunscreens cannot effectively block blue light. Currently, the 27 approved organic sunscreens have no absorption effect on visible light, so organic sunscreens cannot play a role in blocking blue light.

[0007] In the previous large-scale research and screening of natural products, we found that Anthriscus sylvestris has the potential for further development. As a traditional Chinese medicine, Anthriscus sylvestris comes from Anthriscus sylvestris (L.) Hoffm., a plant of the genus Anthriscus in the family Apiaceae. Its roots and leaves are used as medicine. It has the effects of tonifying the middle and replenishing qi, removing blood stasis and promoting new growth. It contains Anthriscus lactone (deoxypodophyllotoxin), iso-Anthriscus lactone, deep yellow celery ketone, (Z)-2-angelica acyloxymethyl-2-butenoic acid, as well as chemical components such as reducing sugar, sucrose, and starch. Although Anthriscus sylvestris has some similar effects to ginseng and Panax notoginseng, the three belong to completely different families and the ingredients they contain are also quite different. At present, the research on Anthriscus sylvestris at home and abroad is gradually increasing. For example, KR1020120055482A discloses an antibacterial composition containing Anthriscus sylvestris extract, which is extracted from Anthriscus sylvestris using one of the alcohols with 1-4 carbon atoms and a mixed solvent thereof. The extract has anti-Helicobacter pylori, Escherichia coli, and Staphylococcus aureus activity. KR1020180074622A discloses a composition for preventing or treating arthritis, which contains the ginseng and honey extract. However, there are still few research reports on the use of ginseng for personal care cosmetics. CN105832624A discloses a Chinese medicine composition for removing acne containing Guanzhong for cosmetics, which combines ginseng with five other Chinese medicines such as Guanzhong for removing acne. KR101724870B1 discloses the use of a combination of ginseng extract and costus root extract to promote hair growth, so as to activate Wnt / field-linked protein signaling in hair follicle cells to stimulate stem cells. Therefore, the application of ginseng in skin care is still under development and research. Summary of the invention

[0008] To solve the above problems, the present invention provides an anti-blue light skin care composition comprising a polysaccharide extract of Eleutherodactyl ginseng. The care composition is suitable for Chinese people with skin type III and above. It can effectively block blue light, reduce the appearance of pigmentation, aging, sensitivity and dryness of the skin caused by blue light, inhibit the increase of IL-6 caused by blue light irradiation of skin tissue, and reduce skin itching caused by blue light.

[0009] The present invention first provides an anti-blue light skin care composition comprising an extract of Codonopsis pilosula, which comprises an anti-blue light active ingredient and an excipient acceptable for skin care. The anti-blue light active ingredient comprises a Codonopsis pilosula polysaccharide extract, and the Codonopsis pilosula polysaccharide extract is prepared by the following method: Codonopsis pilosula is extracted with water at pH 3.0-6.0, protease and cellulase are added to the water extract for enzymolysis, the enzymes are inactivated, the supernatant is taken, and an ethanol solution is added to the supernatant for alcohol precipitation to obtain a crude Codonopsis pilosula polysaccharide precipitate; then the crude Codonopsis pilosula polysaccharide precipitate is separated and purified by anion exchange resin, and gradient eluted with distilled water, 0.1-0.2 mol / L, and 0.3-0.4 mol / L NaCl solutions in sequence, and the elution portion of the 0.3-0.4 mol / L NaCl solution is collected to obtain the Codonopsis pilosula polysaccharide extract.

[0010] The protease is preferably papain, and the molar ratio of the protease to the cellulase is preferably (1-3):(1-4), preferably 1:1.5.

[0011] The anion exchange resin is a weakly basic anion exchange resin, preferably DEAE-52 cellulose column chromatography.

[0012] The ginseng is preferably extracted with water at pH = 4.0, 5.0, or 6.0. The ginseng crude polysaccharide precipitate is further decolorized, preferably with hydrogen peroxide.

[0013] The separation and purification is to perform gradient elution with distilled water, 0.2 mol / L and 0.4 mol / L NaCl solutions in sequence, and collect the elution portion of the 0.4 mol / L NaCl solution. The polysaccharide extract of the ginseng contains acidic polysaccharides.

[0014] In terms of weight percentage, the anti-blue light skin care composition contains 0.1-5% of the polysaccharide extract of Glehnia littoralis, preferably 0.5%, 1%, 1.5%, or 2% of the polysaccharide extract of Glehnia littoralis.

[0015] The anti-blue light skin care composition of the present invention can be made into one of lotion, moisturizing cream, hand cream, skin lotion, softener, toner, skin care lotion, moisturizing lotion, nourishing lotion, facial mask and cream.

[0016] The present invention also provides the use of the anti-blue light skin care composition in the preparation of a crowd care product or cosmetic for preventing skin pigmentation and aging caused by blue light.

[0017] The population is selected from the group consisting of skin types III, IV, V and VI.

[0018] The anti-blue light skin care composition of the present invention also reduces the pigmentation, aging, sensitivity and dryness of the skin caused by blue light, inhibits the increase of IL-6 caused by blue light irradiation of skin tissue, and reduces the skin itching caused by blue light. Furthermore, the anti-blue light skin care composition provided by the present invention is used in the preparation of a composition for assisting in reducing the skin itching caused by blue light.

[0019] The present invention further provides an anti-blue light skin care composition comprising an extract of Codonopsis pilosula, which comprises an anti-blue light active ingredient and an auxiliary material acceptable for skin care. The anti-blue light active ingredient comprises the above-mentioned Codonopsis pilosula polysaccharide extract and crocin.

[0020] In terms of weight percentage, the anti-blue light skin care composition contains 0.1-5% of the polysaccharide extract of Glehnia littoralis and 0.1-0.8% of crocin, preferably 0.5%, 1%, 1.5%, 2% of the polysaccharide extract of Glehnia littoralis, and preferably 0.5% of crocin.

[0021] The invention provides an application of the anti-blue light skin care composition in the preparation of cosmetics or skin care compositions that improve resistance to blue light damage. Or provides an application of crocin in the preparation of cosmetics or skin care compositions that improve resistance to blue light damage and blue light blocking rate of ginseng polysaccharide extract.

[0022] The present invention further provides an anti-blue light skin care composition comprising an extract of Pieris japonica, which comprises an anti-blue light active ingredient and excipients acceptable for skin care. The anti-blue light active ingredient comprises the above-mentioned Pieris japonica polysaccharide extract and gardenia glycoside.

[0023] In terms of weight percentage, the anti-blue light skin care composition contains 0.1-5% of the polysaccharide extract of Eupatorium chinense and 0.1-0.8% of gardenia glycoside, preferably 0.5%, 1%, 1.5%, 2% of the polysaccharide extract of Eupatorium chinense, and preferably 0.5% of gardenia glycoside.

[0024] The invention provides an application of the anti-blue light skin care composition in the preparation of cosmetics or skin care compositions that improve resistance to blue light damage. Or provides an application of geniposide in the preparation of cosmetics or skin care compositions that improve resistance to blue light damage and blue light blocking rate of ginseng polysaccharide extract.

[0025] The present invention further provides an anti-blue light skin care composition comprising an extract of Codonopsis pilosula, which comprises an anti-blue light active ingredient and an excipient acceptable for skin care, wherein the anti-blue light active ingredient comprises the above-mentioned Codonopsis pilosula polysaccharide extract and lycopene. The lycopene is selected from one or more of phytoene and phytoene.

[0026] In terms of weight percentage, the anti-blue light skin care composition contains 0.1-5% of the polysaccharide extract of Glehnia littoralis and 0.1-0.8% of lycopene, preferably 0.5%, 1%, 1.5%, 2% of the polysaccharide extract of Glehnia littoralis, and preferably 0.5% of lycopene.

[0027] The invention provides an application of the anti-blue light skin care composition in the preparation of cosmetics or skin care compositions that improve the resistance to blue light damage. Or provides an application of lycopene in the preparation of cosmetics or skin care compositions that improve the resistance to blue light damage and blue light blocking rate of ginseng polysaccharide extract.

[0028] The anti-blue light active ingredients of the present invention further include cyanidin-3-O-glucoside and delphinidin-3-O-glucoside.

[0029] The composition of the present invention also includes an oil phase, which includes oil, preferably vegetable oil, animal oil, mineral oil, or other oils such as volatile silicone oil and volatile non-silicone oil.

[0030] Preferred are soybean oil, sesame oil, corn oil, almond oil, castor oil, almond oil, palm oil, grape seed oil, rapeseed oil, sunflower oil, cottonseed oil, avocado oil, jojoba oil, olive oil or grain germ oil, shea butter resin, liquid paraffin (or vaseline), squalene, perhydrosqualene, hydrogenated polyisobutene, mink oil, turtle oil. Or selected from isopropyl myristate, isononyl isononanoate, isopropyl palmitate, butyl stearate, hexyl laurate, diisopropyl adipate, 2-ethylhexyl palmitate, 2-hexyldecyl laurate, 2-octyldecyl palmitate, cetyl alcohol, oleyl alcohol, behenyl alcohol.

[0031] The silicone oil is selected from the group consisting of dimethicone, phenyl trimethicone, phenyl dimethicone, octamethylcyclotetrasiloxane, decamethylcyclopentasiloxane, dodecamethylcyclohexasiloxane, heptamethylhexyltrisiloxane, heptamethyloctyltrisiloxane, octamethyltrisiloxane and decamethyltetrasiloxane, and mixtures thereof. Other volatile oils are selected from the group consisting of isododecane, isodecane and isohexadecane.

[0032] The preferred embodiment of the present invention also includes a surfactant, including a nonionic, anionic, amphoteric or zwitterionic surfactant. The nonionic surfactant is selected from alkyl polyglycosides such as alkyl polyglucosides, octyl glucoside, lauryl glucoside, cocoyl glucoside or oxyethylene / oxypropylene block polymers or polysorbates such as Tween-20, Tween-60 or Tween-80. The anionic surfactant is selected from alkyl sulfates, alkyl ether sulfates and salts thereof such as sodium laureth sulfate and magnesium laureth sulfate. The zwitterionic surfactant is selected from disodium cocoamphodiacetate, sodium cocoamphoacetate or cocamide DEA.

[0033] In addition to the above components, it may further include emulsifiers such as PEG-40 stearate, ceteareth-20, lecithin, hydrogenated lecithin, PEG-20 glyceryl isostearate, polyglyceryl-3-methylglucose distearate, and polyglyceryl-10 stearate.

[0034] The composition of the present invention further comprises an aqueous phase, which comprises a humectant and / or a solvent, such as one or more selected from butylene glycol, pentylene glycol, glycerol, butylene glycol, and ethylhexylglycerin.

[0035] The aqueous phase may further contain a chelating agent, the chelating agent being present in an amount of 0.01% to 0.1% by weight, preferably 0.05% relative to the total weight of the composition. The chelating agent may be selected from EDTA, tartaric acid, ascorbic acid, sodium metaphosphate, and galactaric acid.

[0036] The present invention further includes whitening agents and / or sunscreens, such as vitamin B6, niacin, niacinamide, nicotinic acid, vitamin C, vitamin A, etc., to provide additional skin whitening effects. Other whitening agents such as green tea extract, aloe extract, lemon extract, azelaic acid, butylated hydroxyanisole, gluconic acid, glycolic acid, ammonium lactate, ursolic acid, ellagic acid, kojic acid, lactic acid, linoleic acid, butylated hydroxytoluene, citrate, 2,5-di-hydroxybenzoic acid, 2-(4-hydroxyphenyl)-1,3-dithiane, glucopyranyl-1-ascorbic acid, hydroquinone, 4-hydroxyanisole, 4-hydroxybenzoic acid derivatives, hydroxyoctanoic acid, inositol ascorbate, magnesium ascorbyl phosphate, 5-octanoyl salicylic acid and mixtures thereof may also be selected. The sunscreen is selected from 2-ethylhexyl-p-methoxycinnamate, 2-hydroxy-4-methoxybenzophenone, octyldimethyl-p-aminobenzoic acid, 4-tert-butyl-4'-methoxydibenzoylmethane, 2-ethylhexyl salicylate, digalloyl trioleate, 2,2-dihydroxy-4-methoxybenzophenone, ethyl-4-(bis(hydroxypropyl))aminobenzoate, glyceryl-p-aminobenzoate, 3,3,5-trimethylcyclohexyl salicylate, aminobenzoate, 2-ethylhexyl-p-dimethylaminobenzoate, and 2-phenylbenzimidazole-5-sulfonic acid.

[0037] The aqueous phase may further include antioxidants such as vitamin C, retinol, vitamin E, and the like.

[0038] The composition of the present invention may contain a hydrophilic gelling agent such as xanthan gum, guar gum, carboxyvinyl polymer (carbomer), acrylic acid copolymers such as acrylate / alkyl acrylate copolymers, polyacrylamide, hydroxypropyl cellulose, preferably in an amount of 0.01% to 0.5% by weight relative to the total weight of the composition.

[0039] The composition of the present invention further comprises 45 to 90 wt % of purified water, more preferably 50 to 80 wt %, still more preferably 60 to 70 wt %.

[0040] When the composition of the present invention is made into a cream, it contains fatty acid soaps such as alkali metal salts of C14 to C18 fatty acids, such as sodium salts or potassium salts, preferably alkali metal salts of stearic acid and palmitic acid, preferably containing 0.5 to 10 wt%, 1 to 5 wt% of fatty acid soap.

[0041] In a preferred embodiment, the composition of the present invention further comprises 0.1% to 3wt% of a filler, preferably talc, mica, silica, kaolin, laponite, polyamide powder, polyurethane powder, silicone resin microbeads, precipitated calcium carbonate, calcium hydrogen phosphate, magnesium carbonate, magnesium hydrogen carbonate, hydroxyapatite, hollow silica microspheres. The present invention further comprises a preservative. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] Figure 1 Protective effect of ESP-02 on apoptosis of HaCaT cells irradiated with blue light.

[0043] Figure 2 Effect of ESP-02 on IL-6 secretion in HaCaT cells irradiated with blue light.

[0044] Example 1 Extraction and purification of Polysaccharides from Glehnia littoralis

[0045] Extraction: Slice the dried ginseng, weigh an appropriate amount, add 30 times the amount of distilled water, adjust the pH to 5.0, heat and reflux for 3 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, add a certain amount of enzyme (mpapain: mcellulase = 1:1.5), enzymolysis at 60℃ for 2 hours, inactivate the enzyme, centrifuge at 4000rpm for 30 minutes, take the supernatant, concentrate by rotary evaporation to a density of 1.15g / ml, add 95% ethanol to an ethanol concentration of 80% (v / v), let stand at 4℃ for 12 hours, centrifuge at 4000rpm for 30 minutes, re-dissolve the precipitate in water (to a crude ginseng polysaccharide concentration of 6 mg / ml), decolorize with H2O2 to obtain a crude ginseng polysaccharide aqueous solution.

[0046] Purification: DEAE-52 cellulose column chromatography was used to separate and purify the polysaccharides of Echinops chinensis. Gradient elution was performed with distilled water, and 0.2, 0.4, 0.6 and 0.8 mol / L NaCl solutions, respectively. The fractions were collected using an automatic fraction collector and the absorbance was measured using the phenol-sulfuric acid method. Four polysaccharide fractions (ESP-01, ESP-02, ESP-03 and ESP-04) of different gradient eluates of sodium chloride were collected, concentrated by rotary evaporation at 60°C, and dialyzed for 48 hours to obtain four different elution parts of Echinops chinensis polysaccharides.

[0047] Example 2 DPPH free radical scavenging experiment

[0048] Preparation of DPPH solution: Take 2 mg of DPPH powder, weigh accurately, and dilute to 50 ml in a volumetric flask with anhydrous ethanol. Protect from light and store at 4 °C for later use.

[0049] According to the reaction system in Table 1, add the sample to a 96-well plate, place it in a shaker at room temperature and away from light to mix for 30 minutes, and measure the absorbance at 517nm on an ELISA reader, using Vc as a positive control. Repeat three times, calculate the clearance rate, and use SPSS25.0 software to calculate IC 50 value and IC index.

[0050] Table 1 Reaction solution composition and dosage (μL) of DPPH free radical scavenging experiment

[0051]

[0052]

[0053] DPPH free radical scavenging rate = [(AD)-(CD)] / (AD)×100%, where A, C, and D are the absorbance values ​​measured for the above groups, respectively.

[0054] The results showed that the IC values ​​of different ESP-01, ESP-02, ESP-03 and ESP-04 for scavenging DPPH free radicals were 50 The values ​​are shown in Table 2, and the order of their removal abilities is ESP-02>ESP-01>ESP-03>ESP-04. The present invention takes ESP-02 as the subsequent research object.

[0055] Table 2 IC50 values ​​of different ginseng polysaccharides in scavenging free radicals

[0056]

[0057] Example 3 Study on the protective effect of ESP-02 polysaccharide on apoptosis of HaCaT cells irradiated with blue light

[0058] HaCaT cells were cultured at 5*10 3 The cells were inoculated into a 96-well plate at a density of 10 cells / well. When the cell confluence reached 60% to 70%, the culture medium was discarded and the cells were irradiated with a blue light irradiator (430 nm to 440 nm) at 40 J / cm 2 The HaCaT cells were irradiated according to the dosage in Table 4. After irradiation, the cells were placed in an incubator at 37°C and 5% CO2 for 24 hours, the culture medium was discarded, the cells were washed three times with PBS, 10 μL of MTT solution was added to each well, the cells were cultured for 4 hours, the cell supernatant was discarded, 150 μL of DMSO (analytical grade) solution was added to each well, the absorbance OD value of each well was measured at 490 nm, and the relative activity of each group of cells was calculated according to the following formula.

[0059]

[0060] A: Control group

[0061] B: Drug administration group (or model group)

[0062] Table 4 Experimental groups and treatment methods

[0063]

[0064]

[0065] The experimental results showed that the model group was significantly different from the control group (***P<0.001); the 1%, 2% and 4% ESP-02 groups were significantly different from the model group. Therefore, blue light irradiation of HaCaT cells will cause apoptosis, and different concentrations of ESP-02 can protect HaCaT cells from apoptosis caused by blue light irradiation, showing a dose-dependent manner. This shows that ESP-02 has the effect of protecting HaCaT cells from damage caused by blue light irradiation.

[0066] Example 4 Effect of ESP-02 on IL-6 secretion in HaCaT cells irradiated with blue light (ELISA kit detection)

[0067] HaCaT cells were prepared into a single cell suspension and inoculated into a 60 mm dish, 3 mL per well. When the cell confluence reached 60% to 70%, the culture medium was discarded and the cells were irradiated with a blue light irradiator (430 nm to 440 nm) at 40 J / cm 2 The HaCaT cells were irradiated according to the dosage in Table 4. After irradiation, they were placed in an incubator at 37°C and 5% CO2 for 24 hours, and the cell supernatant was collected and the experiment was carried out according to the following steps:

[0068] (1) Sample preparation: The collected cell supernatant was centrifuged at 3000 rpm / min for 10 min, and the precipitate was removed and set aside.

[0069] (2) Reaction steps: Follow the steps in the kit.

[0070] The OD value of each well was measured at 450 nm, and the standard curve (Y = 0.0358X + 0.0289, R 2 =0.9999) to calculate the IL-6 content.

[0071] The results showed that the model group was significantly different from the control group (P<0.01), the 1% and 2% ESP-02 groups were significantly different from the model group (P<0.05), and the 4% ESP-02 group was significantly different from the model group (P<0.01). Therefore, all ESP-02 groups can significantly reduce the secretion of IL-6 in HaCaT cells irradiated with blue light, and the effect is dose-dependent.

[0072] Example 5

[0073] The anti-blue light emulsion is prepared according to the prescription in Table 5, comprising the following steps:

[0074] (1) Weighing each component, adding the active component including EPS-2, propylene glycol, butylene glycol, disodium EDTA, ethylhexylglycerin, and purified water in sequence, heating to 60-70° C., and stirring well to prepare an aqueous phase;

[0075] (2) adding the oil phase components of polydimethylsiloxane, behenyl alcohol, polysorbate 60, isononyl isononanoate, isopropyl myristate, and avocado butter, heating to 70-80° C., and stirring evenly to prepare an oil phase;

[0076] (3) The oil phase and the water phase are mixed and thoroughly homogenized to prepare emulsions A, B, C, and D, respectively.

[0077] Table 5 Emulsion composition of different active ingredients (weight percentage)

[0078]

[0079] Determination of blue light blocking rate: Prepare the cosmetic emulsions of formula A, B, C, and D at 2 mg / cm 2 The amount of the polysaccharide extract was coated on quartz or a bandpass filter, and then irradiated with a blue light source, and the transmitted blue light wavelength region was measured using an SPR-4001 spectroradiometer (Luzchem, Canada). The results showed that the polysaccharide extract of the present invention has a good blue light blocking effect, and after compounding with crocin, gardenia glycoside, lycopene, etc., its blue light blocking rate is significantly improved.

[0080] Table 6 Blue light blocking rate of different prescriptions

[0081] prescription A B C D Blue light blocking rate 71％ 76％ 82％ 85％

[0082] Example 6 Other Examples of Extraction and Purification of Polysaccharides from Echinops chinensis

[0083] Extraction: Slice the dried ginseng, weigh an appropriate amount, add 20 times the amount of distilled water, adjust the pH to 4.5, heat and reflux for 4 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, add a certain amount of enzyme (mpapain: mcellulase = 1:2), enzymolysis at 60℃ for 2 hours, inactivate the enzyme, centrifuge at 4000rpm for 30 minutes, take the supernatant, concentrate by rotary evaporation to a density of 1.15g / ml, add 95% ethanol to an ethanol concentration of 80% (v / v), let stand at 4℃ for 12 hours, centrifuge at 4000rpm for 225 minutes, re-dissolve the precipitate in water (to a crude ginseng polysaccharide concentration of 6 mg / ml), decolorize with H2O2 to obtain a crude ginseng polysaccharide aqueous solution.

[0084] Purification: The polysaccharide of Echinops radix was separated and purified by DEAE-52 cellulose column chromatography, and gradient elution was performed with distilled water, and 0.2 and 0.4 mol / L NaCl solutions in sequence. The fractions were collected by an automatic fraction collector, and the absorbance was measured by the phenol-sulfuric acid method. The elution fraction of 0.4 mol / L NaCl solution was collected, and the eluate was concentrated by rotary evaporation at 65°C and dialyzed for 48 hours to obtain the polysaccharide of Echinops radix.

[0085] Example 7 Other Examples of Extraction and Purification of Polysaccharides from Echinops chinensis

[0086] Extraction: Slice the dried ginseng, weigh an appropriate amount, add 20 times the amount of distilled water, adjust the pH to 5.5, heat and reflux for 4 hours, centrifuge at 4000rpm for 30 minutes, take the supernatant, add a certain amount of enzyme (mpapain: mcellulase = 1:1), enzymolysis at 60℃ for 2 hours, inactivate the enzyme, centrifuge at 4000rpm for 30 minutes, take the supernatant, concentrate by rotary evaporation to a density of 1.15g / ml, add 95% ethanol to an ethanol concentration of 80% (v / v), let stand at 4℃ for 12 hours, centrifuge at 4000rpm for 225 minutes, re-dissolve the precipitate in water (to a crude ginseng polysaccharide concentration of 6 mg / ml), decolorize with H2O2 to obtain a crude ginseng polysaccharide aqueous solution.

[0087] Purification: The polysaccharide of Echinops radix was separated and purified by DEAE-52 cellulose column chromatography, and gradient elution was performed with distilled water, and 0.2 and 0.35 mol / L NaCl solutions in sequence. The fractions were collected by an automatic fraction collector, and the absorbance was measured by the phenol-sulfuric acid method. The elution portion of 0.35 mol / L NaCl solution was collected, and the eluate was concentrated by rotary evaporation at 65°C and dialyzed for 48 hours to obtain the polysaccharide of Echinops radix.

Claims

1. An anti-blue light skin care composition comprising an extract of Radix Glehniae, characterized in that: The invention comprises anti-blue light active ingredients and excipients acceptable for skin care, wherein the anti-blue light active ingredients comprise polysaccharide extract of Glehnia littoralis and crocin, and the polysaccharide extract of Glehnia littoralis is prepared by the following method: extracting Glehnia littoralis with water at pH 5.0, adding papain and cellulase to the aqueous extract for enzymolysis, inactivating the enzymes, taking the supernatant, adding ethanol solution to the supernatant for alcohol precipitation, and obtaining a crude polysaccharide precipitate of Glehnia littoralis; the crude polysaccharide precipitate of Glehnia littoralis is further decolorized, and then the crude polysaccharide precipitate of Glehnia littoralis is separated and purified by DEAE-52 cellulose column chromatography, and gradient eluted with distilled water, 0.2 mol / L NaCl solution and 0.4 mol / L NaCl solution in sequence, collecting the elution portion of the 0.4 mol / L NaCl solution, and obtaining the polysaccharide extract of Glehnia littoralis.

2. The anti-blue light skin care composition according to claim 1, characterized in that: In terms of weight percentage, the anti-blue light skin care composition contains 0.1-5% of the polysaccharide extract of Radix Glehniae and 0.1-0.8% of crocin.

3. The anti-blue light skin care composition according to claim 2, characterized in that: In terms of weight percentage, the anti-blue light skin care composition contains 1-2% of the polysaccharide extract of Radix Glehniae and 0.1-0.5% of crocin.

4. The anti-blue light skin care composition according to claim 2, characterized in that: In terms of weight percentage, the anti-blue light skin care composition contains 1.5% of the polysaccharide extract of Radix Glehniae and 0.5% of crocin.

5. Use of the anti-blue light skin care composition according to claim 1 in the preparation of a care product or cosmetic for preventing skin pigmentation and aging caused by blue light.

6. The use according to claim 5, characterized in that: The population is selected from the group consisting of skin types III, IV, V and VI.

7. Use of the anti-blue light skin care composition according to claim 1 in preparing a composition for assisting in reducing skin itching caused by blue light.

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