Application of galangal polysaccharide in preparation of preparation for improving intestinal flora imbalance

Abstract

The invention belongs to the technical field of biological medicine, and relates to application of galangal polysaccharide in preparation of a preparation for improving intestinal flora imbalance. The galangal polysaccharide provided by the invention can effectively improve the alteration of intestinal flora, especially improve the alteration of intestinal flora induced by hyperuricemia, the hyperuricemia can cause intestinal barrier injury, then the intestinal flora is translocated to induce the alteration of the intestinal flora, and the galangal polysaccharide can up-regulate Occludin and ZO-1, repair the intestinal barrier injury and improve the integrity of the intestinal tract; after the action of the galangal polysaccharide, the influence on the intra-group diversity Beta diversity of the flora is large, the flora structure deviation caused by hypeluricemia can be reversed, and the intestinal flora structure deviation can be relieved. The galangal polysaccharide provided by the invention is a potential novel prebiotic, has an excellent effect in improving intestinal flora imbalance, and has a good application prospect.

Description

Technical Field

[0001] The invention belongs to the technical field of biomedicine and relates to an application of galangal polysaccharide in preparing an agent for improving intestinal flora imbalance. Background Art

[0002] Alpinia officinalis is the rhizome of the ginger plant Alpinia officinalis. It is pungent and hot in nature and belongs to the spleen and stomach meridians. It has the functions of warming the stomach and stopping vomiting, dispelling cold and relieving pain. It is often used for cold pain in the abdomen, vomiting due to stomach cold, belching, acid regurgitation and other symptoms. At present, the research on Alpinia officinalis at home and abroad mainly focuses on chemical components such as flavonoids and aromatic oils and pharmacological functions such as antibacterial, anti-tumor, and hypolipidemic. There are still few studies on Alpinia officinalis polysaccharides. Prior art Chinese patents CN108997510A and CN118027233A respectively disclose the antioxidant application of Alpinia officinalis polysaccharides and their application in improving insulin resistance, showing that Alpinia officinalis polysaccharides have good development and application prospects, and their functional activity needs to be further explored.

[0003] Intestinal dysbiosis refers to a state in which the intestinal flora changes from a physiological combination to a pathological combination, which is manifested by changes in flora diversity, richness and flora composition structure. The degree of dysbiosis varies. Mild imbalance does not cause changes in the body. Moderate imbalance may develop into serious gastrointestinal diseases. Severe dysbiosis can affect various organs and disrupt the balance of the whole body. There are many factors that induce intestinal dysbiosis, and the degree and phenotype of dysbiosis caused by different factors are often different. Studies have found that the occurrence and development of hyperuricemia are closely related to intestinal dysbiosis. Hyperuricemia can damage the intestinal barrier and cause intestinal dysbiosis. At present, improving intestinal dysbiosis and improving hyperuricemia through prebiotics is an effective way.

[0004] Plant polysaccharides are macromolecules, and their oral utilization is low due to molecular weight limitations. In recent years, as research progresses, it has gradually been shown that carbohydrate-active enzymes in the intestinal flora can utilize and decompose them to produce favorable metabolites and achieve the purpose of adjusting the flora structure. Therefore, it is considered to be a potential prebiotic substance. In order to further study the biological activity of galangal polysaccharides, seek regulatory measures to alleviate hyperuricemia-induced intestinal flora imbalance, and explore the application of galangal polysaccharides in improving intestinal flora imbalance, it is of great significance. Summary of the invention

[0005] In order to solve the above technical problems, the present invention separates and extracts galangal polysaccharides from galangal, and uses molecular biology methods and animal models to further study the regulatory effect of galangal polysaccharides on intestinal dysbacteriosis, aiming to provide a new prebiotic substance for improving intestinal dysbacteriosis, provide an application of galangal polysaccharides in the preparation of a modulator for improving intestinal dysbacteriosis, and promote the high-value utilization of galangal polysaccharides.

[0006] In one aspect, the present invention provides an application of galangal polysaccharide in preparing an agent for improving intestinal flora imbalance, wherein the galangal polysaccharide is extracted from galangal.

[0007] Furthermore, in the application, the intestinal flora imbalance is high uric acid induced intestinal flora imbalance; the intestinal flora imbalance includes intestinal barrier damage and / or flora structure deviation.

[0008] Furthermore, in the application, the intestinal barrier damage includes the down-regulation of the expression of tight junction proteins Occludin and / or ZO-1 proteins representing the integrity of the intestinal barrier; the deviation of the flora structure includes significant changes in Beta diversity representing the diversity within and between intestinal flora groups.

[0009] Furthermore, in the application, the galangal polysaccharide is administered by intragastric administration.

[0010] Furthermore, in the application, the galangal polysaccharide repairs intestinal barrier damage induced by high uric acid, and the administration concentration of the galangal polysaccharide is 75 mg / kg to 250 mg / kg.

[0011] Furthermore, in the application, the administration concentration of the galangal polysaccharide is 250 mg / kg.

[0012] Furthermore, in the application, the galangal polysaccharide alleviates the deviation of the flora structure induced by high uric acid, and the alleviation is achieved by regulating the Beta diversity of the intestinal flora.

[0013] Furthermore, in the application, regulating the Beta diversity of the intestinal flora includes downregulating the Firmicutes and / or downregulating the Planctomyces and / or downregulating the Proteobacteria and / or upregulating the Bacteroidetes at the level of intestinal flora composition.

[0014] Furthermore, in the application, the regulation of intestinal flora Beta diversity includes down-regulating the genus Lachnospiraceae_NK4A136_ and / or down-regulating the genus Clostridium and / or up-regulating the genus Ruminococcus and / or up-regulating the genus Eubacterium at the genus level of intestinal flora composition.

[0015] Furthermore, in the application, the preparation of the galangal polysaccharide includes extracting crude galangal polysaccharide from galangal, enzymatically hydrolyzing the galangal polysaccharide with papain to remove protein, and adsorbing the galangal polysaccharide with XAD-16 macroporous resin to remove small molecule peptides and pigments, thereby obtaining galangal polysaccharide.

[0016] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages: (1) The present invention provides a method for preparing galangal polysaccharide by optimizing the process. The galangal polysaccharide prepared by the method has a higher purity.

[0017] (2) The galangal polysaccharide provided by the present invention has excellent effect on improving intestinal flora imbalance in regulating hyperuric acid-induced intestinal flora imbalance.

[0018] (3) The galangal polysaccharide provided by the present invention regulates the Beta diversity of the intestinal flora at the genus level of intestinal flora composition by down-regulating the genus Lachnospiraceae_NK4A136_, down-regulating the genus Clostridium, up-regulating the genus Ruminococcus, and up-regulating the genus Eubacterium, thereby alleviating the deviation of the flora structure induced by high uric acid.

[0019] The galangal polysaccharide provided by the present invention is a novel prebiotic substance for improving intestinal flora imbalance, and can be used in the preparation of an agent for improving intestinal flora imbalance, thereby promoting the high-value utilization of the galangal polysaccharide. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The results of the relative expression of tight junction proteins Occludin and ZO-1 in the colon tissue proteins of mice in different experimental groups. A is the immunoblot protein band diagram of tight junction proteins Occludin and ZO-1 in the colon tissue proteins of mice in different experimental groups; B is the statistical result diagram of the relative expression of tight junction proteins Occludin and ZO-1 in the colon tissue proteins of mice in different experimental groups; * indicates a significant difference compared with the control group (p<0.05), and # indicates a significant difference compared with the model group (p<0.05).

[0021] Figure 2 The results of Beta diversity determination of mice in different experimental groups.

[0022] Figure 3 The results of the main phylum level composition determination of the intestinal flora of mice in different experimental groups. Among them, A is the clustering abundance diagram of the intestinal flora phylum level of mice in different experimental groups; B is the relative abundance expression of Firmicutes in mice in different experimental groups; C is the relative abundance expression of Bacteroidetes in mice in different experimental groups; D is the relative abundance expression ratio of Firmicutes to Bacteroidetes in mice in different experimental groups; E is the relative abundance expression of Planctomycetes in mice in different experimental groups; F is the relative abundance expression of Proteobacteria in mice in different experimental groups. * indicates significant difference (p<0.05).

[0023] Figure 4 The results of the composition of the intestinal flora of mice in different experimental groups at the genus level (ranked in the top ten). Among them, A is the clustering abundance diagram of the intestinal flora of mice in different experimental groups at the genus level; B is the relative abundance expression of the Lachnospiraceae _NK4A136_ genus of mice in different experimental groups; C is the relative abundance expression of the Clostridium genus of mice in different experimental groups; D is the relative abundance expression of the Eubacterium genus of mice in different experimental groups; E is the relative abundance expression of the Ruminococcus genus of mice in different experimental groups; * indicates significant difference (p<0.05). DETAILED DESCRIPTION

[0024] The technical solution of the present invention is described below in conjunction with embodiments; however, the present invention is not limited to the following embodiments.

[0025] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings, but the embodiments are not intended to limit the present invention.

[0026] The experimental methods and detection methods described in the following embodiments are conventional methods unless otherwise specified; the reagents and materials are commercially available unless otherwise specified.

[0027] Alpinia officinalis was purchased from Xuwen County, Guangdong Province.

[0028] Example 1 This example is to prepare a crude galangal polysaccharide solution.

[0029] Use a Chinese herbal medicine grinder to fully grind the galangal, pass it through a 40-mesh sieve to obtain the galangal powder, and place it in a cool and dry place for later use.

[0030] Weigh 300g of galangal powder, add 95% ethanol at a solid-liquid ratio of 1:10 (exemplary 1g galangal powder, 10g 95% ethanol), reflux extraction at 85°C for 1h, filter, collect the filtrate, repeat the extraction of the residue twice according to the above extraction method, combine the filtrates, concentrate at 55°C under reduced pressure to 1 / 5 of the original volume, and store at -20°C for later use; dry the residue at 60°C until the ethanol is completely volatilized, and store at 4°C for subsequent galangal polysaccharide extraction. Accurately weigh 60g of the above-mentioned galangal extraction residue, add deionized water at a solid-liquid ratio of 1:20, and treat at a high temperature of 105°C for 90 minutes to fully gelatinize the starch therein to obtain suspension 1, cool naturally at room temperature, adjust the pH of suspension 1 to 5.5, add 1% (w / w) of the sample weight of α-amylase, and hydrolyze at 60°C for 120 minutes to obtain suspension 2, adjust the pH of suspension 2 to 5.0, add 1% (w / w) of the sample weight of glucoamylase, and at the same time add 1% (w / w) of the sample weight of cellulase, The suspension 3 was adjusted to pH 7.0, and the suspension was treated at 105°C for 2 h. The filtrate was collected by suction and concentrated at 55°C under reduced pressure. A certain amount of anhydrous ethanol was added until the concentration of ethanol in the system was 80% (v / v). The suspension was stirred slowly and evenly, and then allowed to stand at 4°C for 12 h. The suspension was centrifuged at 8000 g for 30 min, and the precipitate was collected. Deionized water was added to redissolve the precipitate and the volume was fixed to 50 mL to obtain a crude polysaccharide solution of galangal polysaccharide.

[0031] Example 2 This example is to prepare galangal polysaccharide crude polysaccharide freeze-dried powder.

[0032] Weigh 300g of galangal powder, add 95% ethanol at a solid-liquid ratio of 1:10 (exemplary 1g galangal powder, 10g 95% ethanol), reflux extraction at 85°C for 1h, filter, collect the filtrate, repeat the extraction of the residue twice according to the above extraction method, combine the filtrates, concentrate at 55°C under reduced pressure to 1 / 5 of the original volume, and store at -20°C for later use; dry the residue at 60°C until the ethanol is completely volatilized, and store at 4°C for subsequent galangal polysaccharide extraction. Accurately weigh 60g of the above-mentioned galangal extraction residue, add deionized water at a solid-liquid ratio of 1:20, and treat at 105°C for 90 minutes to fully gelatinize the starch therein to obtain suspension 1, cool naturally at room temperature, adjust the pH of suspension 1 to 5.5, add 1% (w / w) α-amylase by sample weight, and hydrolyze at 60°C for 120 minutes to obtain suspension 2, adjust the pH of suspension 2 to 5.0, add 1% (w / w) glucoamylase by sample weight, and hydrolyze at 55°C for 16h to fully gelatinize the starch in the sample. Hydrolysis was performed and the dissolution of galangal polysaccharide was accelerated to obtain suspension 3, the pH value of suspension 3 was adjusted to 7.0, high temperature treatment was carried out at 105°C for 2h, suction was filtered and the filtrate was collected, and it was concentrated under reduced pressure at 55°C, and then a certain amount of anhydrous ethanol was added to the system until the concentration of ethanol was 80% (v / v). After slow stirring, the system was allowed to stand at 4°C for 12h, centrifuged at 8000g for 30min, the precipitate was collected, deionized water was added to dissolve the precipitate, and it was concentrated under reduced pressure at 55°C to remove the residual ethanol, and freeze-dried to obtain galangal polysaccharide lyophilized powder.

[0033] Example 3 This example is crude and purified galangal polysaccharide.

[0034] Example 1 and Example 2 obtain crude galangal polysaccharide, which also contains proteins and small molecules. In order to further improve the purity of polysaccharides, deproteinization treatment is required. This embodiment uses enzymatic protein removal, specifically adding papain with a sample content of 1% to the crude galangal polysaccharide solution for enzymatic hydrolysis at 37°C for 4 hours, then using dialysis to remove salts, and then adding XAD-16 macroporous resin, 37°C, 120rpm, constant temperature static adsorption for 3 hours, removing small molecule peptides and some pigments, and then filtering and collecting the filtrate, 55°C reduced pressure concentration, to obtain galangal polysaccharide.

[0035] Example 4 This example is an animal experiment on the improving effect of galangal polysaccharide on mice with intestinal flora imbalance induced by hyperuric acid.

[0036] 1. Establish the test model Thirty male SPF Kunming mice (27±2g, 7 weeks old) were randomly divided into 5 groups (control group, model group, low-dose galangal polysaccharide group, medium-dose galangal polysaccharide group, and high-dose galangal polysaccharide group), with 6 mice in each group. The oral doses of the low, medium, and high-dose galangal polysaccharide groups were 75mg / kg, 150mg / kg, and 250mg / kg, respectively. The galangal polysaccharide solution was gavaged in the morning, and 75mg / kg adenine solution and 300mg / kg potassium oxonate solution were gavaged in the afternoon. The model group was gavaged with 0.1mL / 10g normal saline in the morning, and 75mg / kg adenine solution and 300mg / kg potassium oxonate solution were gavaged in the afternoon. The control group was gavaged with 0.1mL / 10g normal saline in the morning and afternoon. After the gavage of each group of mice every day, the mice in each group were free to eat, and the experimental groups (control group, model group, and galangal polysaccharide group) were gavaged for 21 consecutive days. After the last oral administration, the mice were sacrificed and the colon tissues and cecal contents were collected.

[0037] 2. Determination of relative expression of occludin and ZO-1 in mouse colon tissue Tight junction proteins are important indicators for maintaining the integrity of the intestinal barrier. Occludin and zonula occludens-1 (ZO-1) are common tight junction proteins that play a key role in maintaining intestinal barrier function and other physiological barriers.

[0038] Take 20 mg of colon tissue and add RIPA strong lysis buffer containing PMSF at a ratio of 1:100 and phosphatase inhibitor at a ratio of 1:50 (purchased from Shanghai Biyuntian Biotechnology Co., Ltd.). After grinding, lyse at 4°C for 20 minutes, centrifuge at 4°C and 12000rpm for 20 minutes to collect the supernatant. Then use a protein quantification kit to determine the concentration of each sample (purchased from Shanghai Biyuntian Biotechnology Co., Ltd.), adjust it to be consistent, and add 5×SDS loading buffer. Then denature the protein at 100°C for 10 minutes. Use SDS-PAGE electrophoresis to separate the protein, then transfer the protein to a nitrocellulose membrane, block it at room temperature for 1 hour, and incubate it with the primary antibodies GAPDH, Occludin and ZO-1 (antibody dilution of 1:1000) at 4°C overnight. Then incubate with the corresponding secondary antibody (dilution 1:3000) at room temperature for 1 hour and then immerse in the ultra-sensitive ELC working solution. Use a chemiluminescence system for imaging to obtain the protein band diagram as shown below. Figure 1 As shown in A. The grayscale analysis of protein bands was performed using ImageJ software, and the final data was presented as the ratio of the grayscale of the target protein to the grayscale of the internal reference GAPDH. The relative expression of Occludin and ZO-1 in mouse colon tissue is shown in Figure 1 As shown in B.

[0039] Figure 1The results of B in the experiment showed that compared with the control group, the tight junction proteins Occludin and ZO-1 in the colon tissue of the hyperuric acid exposure model group were significantly reduced, indicating that hyperuric acid caused damage to the intestinal physical barrier and destroyed its integrity. After the action of galangal polysaccharide, the expression of tight junction proteins was upregulated. Under the action of the low-dose galangal polysaccharide group, the medium-dose galangal polysaccharide group, and the high-dose galangal polysaccharide group, the expression of ZO-1 was upregulated by 1.9, 1.4, and 1.5 times, respectively, and the expression of Occludin was upregulated by 2.3, 1.8, and 1.4 times, respectively, improving intestinal integrity. The results show that galangal polysaccharide can effectively repair the intestinal barrier damage induced by hyperuric acid and slow down the occurrence of intestinal flora imbalance, among which the high-dose galangal polysaccharide group had the best effect.

[0040] 3. High-throughput sequencing of 16S rDNA in mouse cecal contents After sacrificing the mice in the control group, model group and galangal polysaccharide group (250 mg / kg), the cecal contents were collected and genomic DNA was extracted from the contents using a nucleic acid extraction kit. The 16SrDNA V4 region was PCR amplified using universal primers 515F and 806R for the qualified DNA samples. The sequence of 515F is shown in SEQ ID NO: 1, and the sequence of 806R is shown in SEQ ID NO: 2.

[0041] After purification and quantification, the PCR products were merged into the IlluminaNovaSeq sequencing platform for double-end sequencing to obtain the original sequence. ASVs were clustered at a specific similarity, and the clustering results were annotated and abundance analyzed for representative sequences using Qiime2. Qiime2 and R package (v3.2.0) were further used for sequence data analysis. After the quality assessment of the microbial samples, the intestinal microbial diversity analysis and the changes in the intestinal microbial species composition at the phylum and genus levels were performed. The diversity results are shown in Tables 1 and Figure 2 The changes in bacterial species composition are shown in Figure 3 and Figure 4 shown.

[0042] Alpha diversity and Beta diversity are indicators for evaluating the diversity changes within and between intestinal flora groups, among which Alpha diversity is often shown by Chao1, ACE, Pielou_e, Shannon and Simpson indexes. As shown in Table 1, the Alpha diversity of mice in each group did not change much, and there were no significant differences in Chao1 and ACE indexes among the groups, indicating that high uric acid exposure had little effect on the overall richness and diversity of flora species groups, and it was a chronic intestinal flora imbalance model. All indexes improved after the intervention of galangal polysaccharide, suggesting that galangal polysaccharide improves the diversity of flora groups by enriching dominant flora.

[0043] Table 1 Determination results of Alpha diversity index of intestinal flora of mice in different experimental groups

[0044] Note: Different lowercase letters indicate significant differences Depend on Figure 2 It can be seen that the microbiota structure of the control group and the model group deviated greatly, indicating that high uric acid exposure caused intestinal flora imbalance. After the action of galangal polysaccharide, Beta diversity can be adjusted to improve the microbiota structure.

[0045] Figure 3 and Figure 4 The changes in the composition of the intestinal flora at the phylum and genus levels are shown respectively. Figure 3 From the abundance graph of cluster A in the figure, we can see that high uric acid exposure disrupts the balance of intestinal flora, and the abundance of a large number of microorganisms changes, which can be reversed after intervention with galangal polysaccharides. At the phylum level in each group of mice, Firmicutes and Bacteroidetes account for the vast majority of the flora composition, and the ratio of Firmicutes to Bacteroidetes is an important indicator for measuring the balance of intestinal flora.

[0046] from Figure 3 B and Figure 3 From the C in Figure 1, we can see that high uric acid exposure increased the abundance of Firmicutes and decreased the abundance of Bacteroidetes. Figure 3 As shown in D, the ratio of Firmicutes (F) / Bacteroidetes (B) increased, indicating the occurrence of intestinal flora imbalance in the body. Figure 3 ZhongE and Figure 3 F in the middle shows that the relative abundance of Proteobacteria and Planctomyces, which contain more potential pathogens, was significantly increased after exposure to high uric acid. The F / B ratio and the composition abundance of Proteobacteria and Planctomyces can be reduced to a certain extent after the action of galangal polysaccharides. When analyzing the composition abundance at the genus level, Figure 4As shown in A, the genus Lachnospiraceae_NK4A136_ accounted for the largest proportion at the genus level in each group of mice, followed by Lactobacillus, Clostridium, Enterobacter, Anaerobic Rod Bacteria and Enterobacter. Among the top 10 genera, high uric acid exposure increased the abundance of Lachnospiraceae_NK4A136_ and Clostridium, and downregulated the abundance of Ruminococcus and Eubacterium. Lachnospiraceae_NK4A136_ has been shown to be a potential biomarker for exposure to nanoplastics in food, and has a large correlation with hepatotoxicity. In addition, Lachnospiraceae_NK4A136 plays an important role in D-galactose-induced intestinal flora disorders in mice, and downregulating its abundance can improve intestinal flora disorders. Clostridium is widely distributed in nature. Common pathogenic anaerobic Clostridium species include Clostridium tetani, Clostridium perfringens, Clostridium botulinum and Clostridium difficile, which are closely related to diseases such as gas gangrene and pseudomembranous colitis. Ruminococcus plays an important role in digesting resistant starch, but is also associated with intestinal diseases (ulcerative colitis, Crohn's, etc.), immune diseases (allergies, eczema, asthma, etc.), nervous system diseases (autism, depression, etc.) and liver diseases (metabolic fatty liver disease). Eubacterium plays a key role in cholesterol conversion, oxalate catabolism, and insulin degradation. Therefore, its reduction or deficiency can lead to depression, obesity, diabetes, colorectal cancer, autism, cardiovascular and cerebrovascular diseases, and other diseases. From the results, it can be seen that high uric acid exposure can induce the upregulation of multiple potential pathogens and cause the downregulation of beneficial bacteria, disrupting the original bacterial community structure. After the action of galangal polysaccharides, the abundance of various potential harmful bacteria caused by high uric acid exposure can be downregulated, showing its great potential as an emerging prebiotic.

[0047] As described above, the basic principle, main features and advantages of the present invention are well described. The above embodiments and descriptions are only descriptions of the preferred embodiments of the present invention, and the present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, various changes and improvements made by ordinary technicians in this field to the technical solution of the present invention should fall within the protection scope determined by the present invention.

Claims

1. An application of galangal polysaccharide in the preparation of an agent for improving intestinal flora imbalance, characterized in that: The galangal polysaccharide is extracted from galangal.

2. The use according to claim 1, characterized in that: The intestinal flora imbalance is intestinal flora imbalance induced by high uric acid; The intestinal flora imbalance includes intestinal barrier damage and / or flora structure deviation.

3. The use according to claim 2, characterized in that: The intestinal barrier damage includes down-regulation of tight junction protein Occludin and / or ZO-1 protein expression representing intestinal barrier integrity; The deviations in the microbiota structure included significant changes in Beta diversity, which represents the diversity within and between intestinal microbiota groups.

4. The use according to claim 1, characterized in that: The galangal polysaccharide is administered by intragastric administration.

5. The use according to claim 1, characterized in that: The galangal polysaccharide repairs intestinal barrier damage induced by high uric acid, and the administration concentration of the galangal polysaccharide is 75 mg / kg to 250 mg / kg.

6. The use according to claim 5, characterized in that: The administration concentration of the galangal polysaccharide is 250 mg / kg.

7. The use according to claim 1, characterized in that: The galangal polysaccharide alleviates the deviation of the microbiota structure induced by high uric acid, and the alleviation is achieved by regulating the Beta diversity of the intestinal microbiota.

8. The use according to claim 7, characterized in that: The regulation of intestinal flora Beta diversity includes down-regulating Firmicutes and / or down-regulating Planctomyces and / or down-regulating Proteobacteria and / or up-regulating Bacteroidetes at the intestinal flora composition phylum level.

9. The use according to claim 8, characterized in that: The regulation of intestinal flora Beta diversity includes down-regulating the genus Lachnospiraceae_NK4A136_ and / or down-regulating the genus Clostridium and / or up-regulating the genus Ruminococcus and / or up-regulating the genus Eubacterium at the genus level of intestinal flora composition.

10. The use according to claim 1, characterized in that: The preparation method of galangal polysaccharide comprises the following steps: extracting crude galangal polysaccharide from galangal, enzymolyzing with papain to remove protein, and adsorbing with XAD-16 macroporous resin to remove small molecule peptides and pigments, so as to prepare galangal polysaccharide.

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