Device and method for preparing intestinal micro-ecological washing bacteria

Abstract

The invention discloses a device and a method for preparing intestinal micro-ecological washing bacteria. The device comprises a box body, a feeding device, a raw material treatment system, a nitrogen system, a water supply system, a vacuum split charging bottle and a control system, the stirring device, the filtering device, the separating device, the sub-packaging device and the sewage tank are arranged. The device has the functions that the device can be automatically cleaned while a mixed solution is filtered, so that the operation procedures are reduced, and the external environment is prevented from being polluted in the extraction process; the filtration and extraction efficiency of the bacterial liquid is improved; and the extracted bacterial liquid can be distributed and filled into the bacterial liquid bottles with different specifications, and meanwhile, the residual bacterial liquid and gas during subpackaging can be prevented from escaping to the outside to cause pollution.

Description

Technical Field

[0001] The invention belongs to the technical field of biological equipment, and in particular relates to a device and method for preparing intestinal microecological cleaning bacteria. Background Art

[0002] In the human intestine, there are intestinal flora, which play an important role in the human environment. They can maintain intestinal flora, promote body metabolism, repair intestinal barrier, inhibit harmful flora, and improve immunity. Intestinal flora transplantation is a medical therapy that adjusts and restores the intestinal microbial community of a patient by transferring the flora from the feces of a healthy person to the patient's intestines. Its principle is based on the important role of intestinal flora in maintaining human health and immune function. In this technology, fresh fecal samples need to be obtained from the donor and processed. The processing process usually includes steps such as dilution, stirring and filtration to obtain a liquid or suspension containing the flora.

[0003] In the prior art one: a device for separating and concentrating intestinal flora and a method for preparing the flora are disclosed in a Chinese patent (CN118371048A), which belongs to the field of biological extraction technology. The separation and concentration device includes a flora extraction device and a negative pressure drainer. The flora extraction device includes multiple filters connected in sequence from top to bottom, and two adjacent filters are detachably connected. The mesh size of the screen of each level of filter is gradually reduced from the uppermost level to the lowermost level. The lowermost filter is used to enrich and concentrate the intestinal flora suspension separated by the upper-level filter. The negative pressure drainer is connected to the lowermost filter to collect the liquid generated by the enrichment and concentration process of the lowermost filter; the separation and concentration device integrates a homogenization device, a multi-stage filtration device and an enrichment and concentration device into a whole, simplifies the process of flora separation and concentration, and improves the extraction efficiency of the intestinal flora.

[0004] Using this method, the device cannot automatically discharge the residual filtrate on each level of filters during the separation and filtration process when filtering the mixed liquid to extract the bacterial concentrate. It is necessary to disassemble the filters at each level after the filtration and extraction is completed and then manually clean them separately, which is time-consuming and labor-intensive and adds additional steps. At the same time, the filtrate will also pollute the surrounding environment during the cleaning process.

[0005] In the second prior art: For example, Chinese patent (CN113136319A) discloses a device and method for preparing intestinal microecological washing bacteria, including a toilet, a stirring box and a bacteria collection box. The lower part of the toilet is connected to two stirring boxes through a connecting pipe, and a stirring blade is provided in the stirring box. The lower part of the toilet is also provided with a sewage outlet. The stirring boxes are respectively connected to a water inlet pipe, a sewage pipe and a collecting pipe. One of the stirring boxes is also connected to a nitrogen input pipe. The connecting pipe, the water inlet pipe, the sewage pipe and the collecting pipe are all provided with switches. A retention membrane is provided at the connection position of the stirring box and the collecting pipe. The collecting pipe is connected to the bacteria collection box, so that fresh washing bacteria derived from feces can be obtained as quickly as possible to obtain beneficial intestinal flora.

[0006] In this way, during the process of filtering and extracting the bacterial mixture, as the working time increases, impurities and mucus attached to the filtering device will accumulate at the bottom of the filter screen, resulting in a decrease in the filtering efficiency of the filter screen and thus a decrease in the filtering and extraction efficiency of the device; and this method cannot distribute the extracted bacterial solution into bacterial solution bottles of different specifications according to different experimental preparation conditions, and after the collection box is taken out, the residual bacterial solution and gas in the pipe connected to the collection box will escape to the outside and cause pollution.

[0007] In summary, the following defects exist in the prior art: First, in the process of filtering the mixed liquid to extract the bacterial concentrate, the residual filtrate on each level of the filter cannot be automatically discharged during the separation and filtration. After the filtration and extraction are completed, the filters at each level need to be disassembled and manually cleaned separately, which is time-consuming and labor-intensive, and adds additional steps. At the same time, the filtrate will also pollute the surrounding environment during the cleaning process; Second, as the working time increases, impurities and mucus attached to the filtration device will accumulate at the bottom of the filter, resulting in a decrease in the filtration efficiency of the filter, and thus reducing the filtration and extraction efficiency of the device; Third, the extracted bacterial liquid cannot be distributed and filled into bacterial liquid bottles of different specifications according to different experimental preparation conditions, and after the collection box is taken out, the residual bacterial liquid and gas in the pipe connected to the collection box will escape to the outside and cause pollution.

[0008] To this end, this article provides a device and method for preparing intestinal microecological cleaning bacteria. Summary of the invention

[0009] In order to solve the above technical problems, the present invention discloses a device and method for preparing intestinal microecological washing bacteria. The device can automatically clean the mixed liquid while filtering it, thereby reducing the operation process and preventing pollution to the external environment during the extraction process; increasing the filtering and extraction efficiency of the bacterial liquid; and being able to distribute the extracted bacterial liquid into bacterial liquid bottles of different specifications while preventing the residual bacterial liquid and gas from escaping to the outside and causing pollution during the subpackaging.

[0010] In order to achieve the above technical effects, the present invention provides a device for preparing intestinal microecological washing bacteria, including a box, a feeding device, a raw material processing system, a nitrogen system, a water supply system, a vacuum filling bottle, and a control system. The feeding device is arranged inside the upper left side of the box, the raw material processing system is arranged below the feeding device, the nitrogen system is arranged on the upper right side of the box, and the water supply system is arranged on the left side of the nitrogen system. The nitrogen system for providing an oxygen-free environment for the device and the water supply system for providing sterile saline cleaning are respectively connected to the feeding device and the raw material processing system through pipelines. The vacuum filling bottle is arranged below the outlet of the raw material processing system. The control system is respectively connected to the box, the feeding device, and the raw material processing system. The processing system, the nitrogen system, the water supply system, and the vacuum filling bottles are connected by telecommunication; the raw material processing system also includes a stirring device, a filtering device, a separating device, a filling device, and a sewage tank. The stirring device is arranged on the right side of the feeding device and is connected to the feeding device through a pipeline. The filtering device is arranged below the stirring device and is directly opposite to the pipeline outlet below the stirring device. The separating device is arranged on the right side below the filtering device and is connected to the filtering device through a pipeline. The filling device is arranged on the right side of the separating device and the upper inlet of the filling device is connected to the upper outlet of the separating device through a pipeline. The sewage tank is arranged below the filtering device and is respectively connected to the filtering device and the separating device through pipelines.

[0011] Preferably, the filtering device also includes a separation wall, a stirring disk, a rack, a gear, a drive motor, and an electric valve a. The stirring disk is arranged in the middle of the separation wall and is sealed and slidably connected to the separation wall. The rack is arranged below the stirring disk. The gear is arranged on the left side of the rack and meshes with the rack. The output end of the drive motor is connected to the bottom of the rack. The electric valve a is arranged below the stirring disk and connected to the pipe in the middle of the stirring disk, and the bottom of the electric valve a is connected to the sewage tank through a pipe.

[0012] Preferably, the separation wall also includes a separation layer, a drainage wall, and a liquid storage layer. The separation layer with an outwardly expanding annular shape and a rough surface is arranged above the drainage wall, the liquid storage layer is arranged below the drainage wall, and an outlet is arranged at the lower right side of the liquid storage layer and connected to the inlet above the separation device through a pipeline.

[0013] Preferably, the separation device further comprises a separation cylinder, a barrel cover, a centrifugal chassis, a sewage outlet, and a water inlet mechanism. The barrel cover is arranged below the separation cylinder and is sealingly and slidably connected to the separation cylinder. The separation cylinder is arranged as a conical barrel structure which is narrow at the top and bottom and wide in the middle. The centrifugal chassis is arranged below the separation cylinder. The sewage outlet is arranged below the separation cylinder and is connected to the sewage tank through a pipeline passing through the centrifugal chassis. The water inlet mechanism is arranged inside the separation cylinder and is connected to the upper inlet of the filling device through the barrel cover.

[0014] Preferably, the water inlet mechanism also includes a hose, a water inlet, a chute, and a float. The hose is connected to the right side of the water inlet, and the bottom of the chute is connected to the left side of the water inlet. The float is slidably installed inside the chute, and the diameter of the float is larger than the diameter of the inlet at the connection between the water inlet and the chute.

[0015] Preferably, the density of the float is less than the density of the bacterial liquid after stratification and greater than the density of the upper mucus after stratification.

[0016] Preferably, the packaging device also includes a liquid storage box, an electric valve b, a contact switch, a slider, a needle, and a bottle mouth limiting mechanism. The electric valve b is arranged below the liquid storage box, the contact switch is arranged on the left side of the electric valve b and controls the opening and closing of the electric valve b, the slider is arranged below the contact switch, the needle is slidably sleeved on the inner side of the slider, and the bottle mouth limiting mechanism is slidably sleeved below the slider.

[0017] Preferably, the bottle mouth limiting mechanism further includes a limiting block, an access port, and a mark. The limiting blocks are respectively arranged on the front and rear sides of the lower inner side of the bottle mouth limiting mechanism. The gap between the limiting blocks constitutes the access port. An inclined surface is arranged on the limiting block to divide the limiting block into an upper layer and a lower layer. The mark is arranged on the right side of the access port.

[0018] Preferably, the vacuum dispensing bottle is further provided with blocks on the left and right sides, and a mark having the same color as the bottle mouth limiting mechanism is provided on the outer side of the block.

[0019] Based on the above technical solution, the present invention also provides a method for preparing intestinal microecological cleaning bacteria, which specifically comprises the following steps: S1: Put the fresh feces sample into the feeding device, and the nitrogen system introduces nitrogen into the feeding device to ensure an anaerobic environment in the feeding device. Then the water supply system sprays sterile saline to bring the feces into the stirring device; S2: The stool sample and sterile saline are stirred and mixed in a stirring device to form a mixed solution; S3: After the mixed liquid is fully stirred, it is slowly discharged downward into the filtration device. The overflowed liquid and mucus are collected along the surface of the separation layer to the liquid storage layer due to the wall adhesion effect of the liquid. The solid impurities are precipitated to the bottom of the separation layer. At the same time, the driving motor drives the stirring disk to rotate slowly to accelerate the separation process. S4: The mixed liquid after preliminary filtration enters the separation cylinder from the liquid storage layer, and the centrifugal chassis drives the mixed liquid in the separation cylinder to rotate and centrifuge; S5: The nitrogen system introduces nitrogen into the separation cylinder for pressurization, and presses the bacterial liquid in the middle layer into the liquid storage tank of the subpackaging device through the water inlet mechanism floating in the bacterial liquid layer, thereby completing the extraction of the bacterial liquid; S6: Use a suitable vacuum sub-filling bottle, align the mark on the stopper with the mark on the bottle mouth limit mechanism, insert it upward and rotate it counterclockwise, so that the needle is inserted into the vacuum sub-filling bottle to absorb the bacterial solution. When it is almost full, turn the vacuum sub-filling bottle clockwise. After the remaining vacuum pressure in the sub-filling bottle sucks out the remaining bacterial solution in the needle, remove the vacuum sub-filling bottle to complete the extraction; S7: After the bacterial liquid is extracted, the device is rinsed clean with sterile saline through the water supply system, and the flushed sewage is stored in the sewage tank for treatment. The nitrogen system also provides nitrogen to maintain the pressure balance in the device, and the gas is discharged from the top of the sewage tank after filtering.

[0020] Compared with the prior art, the present invention has the following beneficial effects: The device comprises a box body, a feeding device, a raw material processing system, a nitrogen system, a water supply system, and a vacuum filling bottle. The stirring device, filtering device, separating device, filling device, and sewage tank in the raw material processing system are arranged, and the device is connected to the nitrogen system and the water supply system. When filtering the mixed liquid, the impurities filtered in each step can be discharged into the sewage tank respectively, and the device is automatically cleaned to reduce the operation process. At the same time, the device is isolated from the external environment during the processing process to prevent the external environment from being polluted during the extraction process. The filtering device and the separating device are used to filter and extract the bacterial liquid separately, and the bacterial liquid is separated from the mucus and impurities by the wall attachment effect and the centrifugal action of the liquid, so that the filter screen will not be blocked, and the filtering and extraction efficiency of the bacterial liquid is increased. The filling device is arranged, and the extracted bacterial liquid can be distributed and filled into bacterial liquid bottles of different specifications, and the bacterial liquid in the front end of the filling device can be automatically cleaned to prevent the residual bacterial liquid and gas from escaping to the outside world and causing pollution during the filling. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is an isometric view of the present invention; Figure 2 It is a left side view of the present invention; Figure 3 yes Figure 2 Sectional view of section a; Figure 4 yes Figure 3 Partial schematic diagram of middle b; Figure 5 yes Figure 3 Partial schematic diagram of middle c; Figure 6 yes Figure 3 Partial schematic diagram of middle d; Figure 7 It is a process flow chart of the present invention; In the accompanying drawings, the components represented by the reference numerals are listed as follows: 1. Box body; 2. Feeding device; 3. Nitrogen system; 4. Water supply system; 5. Vacuum filling bottle; 6. Stirring device; 7. Filtering device; 8. Separating device; 9. Filling device; 10. Sewage tank; 11. Stirring plate; 12. Rack; 13. Gear; 14. Driving motor; 15. Electric valve a; 16. Separation layer; 17. Drainage wall; 18. Liquid storage layer; 19. Separation cylinder; 20. Barrel cover; 21. Centrifugal chassis; 22. Sewage outlet; 23. Water inlet mechanism; 24. Hose; 25. Water inlet; 26. Chute; 27. Float; 28. Liquid storage tank; 29. ​​Electric valve b; 30. Contact switch; 31. Sliding block; 32. Needle; 33. Limit block; 34. Access port; 35. Stopper. DETAILED DESCRIPTION

[0022] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention. Example 1

[0023] like Figures 1 to 6 As shown: The prior art in this embodiment has the following problems: The inventors have found that the prior art has the following defects: First, in the process of filtering the mixed liquid to extract the bacterial concentrate, the residual filtrate on each level of the filter cannot be automatically discharged during the separation and filtration. After the filtration and extraction are completed, the filters at each level need to be disassembled and manually cleaned separately, which is time-consuming and labor-intensive, and adds additional processes. At the same time, the filtrate will also pollute the surrounding environment during the cleaning process; Second, as the working time increases, impurities and mucus attached to the filtration device will accumulate at the bottom of the filter screen, resulting in a decrease in the filtration efficiency of the filter screen, and thus reducing the filtration and extraction efficiency of the device; Third, the extracted bacterial liquid cannot be distributed and filled into bacterial liquid bottles of different specifications according to different experimental production conditions, and after the collection box is taken out, the residual bacterial liquid and gas in the pipe connected to the collection box will escape to the outside and cause pollution; Therefore, the inventor provides a device for preparing intestinal microecological washing bacteria, including a box body 1, a feeding device 2, a raw material processing system, a nitrogen system 3, a water supply system 4, a vacuum filling bottle 5, and a control system. The feeding device 2 is arranged inside the upper left side of the box body 1, the raw material processing system is arranged below the feeding device 2, the nitrogen system 3 is arranged on the upper right side of the inside of the box body 1, and the water supply system 4 is arranged on the left side of the nitrogen system 3. The nitrogen system 3 that provides an oxygen-free environment for the device and the water supply system 4 that provides sterile saline for cleaning are respectively connected to the feeding device 2 and the raw material processing system through pipelines. The vacuum filling bottle 5 is arranged below the outlet of the raw material processing system. The control system (not shown in the figure) is respectively connected to the box body 1, the feeding device 2, the raw material processing system The system, the nitrogen system 3, the water supply system 4, and the vacuum filling bottle 5 are connected by telecommunication; the raw material processing system also includes a stirring device 6, a filtering device 7, a separating device 8, a filling device 9, and a sewage tank 10. The stirring device 6 is arranged on the right side of the feeding device 2 and is connected to the feeding device 2 through a pipeline. The filtering device 7 is arranged below the stirring device 6 and is directly opposite to the pipeline outlet below the stirring device 6. The separating device 8 is arranged on the right side below the filtering device 7 and is connected to the filtering device 7 through a pipeline. The filling device 9 is arranged on the right side of the separating device 8 and the upper inlet of the filling device 9 is connected to the upper outlet of the separating device 8 through a pipeline. The sewage tank 10 is arranged below the filtering device 7 and is connected to the filtering device 7 and the separating device 8 through a pipeline.

[0024] Further, the filtering device 7 also includes a separation wall, a stirring disc 11, a rack 12, a gear 13, a driving motor 14, and an electric valve a15. The stirring disc 11 is arranged in the middle of the separation wall and is sealed and slidably connected with the separation wall. The rack 12 is arranged below the stirring disc 11. The gear 13 is arranged on the left side of the rack 12 and meshes with the rack 12. The output end of the driving motor 14 is connected to the bottom of the rack 12. The electric valve a15 is arranged below the stirring disc 11 and connected to the pipeline in the middle of the stirring disc 11, and the bottom of the electric valve a15 is connected to the sewage tank 10 through a pipeline. Among them, the driving motor 14 drives the rack 12 to rotate through the gear 13, and the stirring plate 11 can drive the mixed liquid above to rotate, so that the impurities are slowly precipitated by the centrifugal effect, and at the same time, the liquid in the mixed liquid is pushed to flow down to the separation wall to complete the preliminary filtration. This method uses centrifugal action and wall attachment effect to initially separate and filter solid impurities and liquid, so that the filter screen will not be blocked, thereby increasing the filtration and extraction efficiency of the bacterial liquid.

[0025] Furthermore, the separation wall further comprises a separation layer 16, a drainage wall 17, and a liquid storage layer 18. The separation layer 16 with an outwardly expanding ring shape and a rough surface is arranged above the drainage wall 17, and the liquid storage layer 18 is arranged below the drainage wall 17. An outlet is arranged at the lower right side of the liquid storage layer 18 and is connected to the upper inlet of the separation device 8 through a pipeline. The rough surface of the separation wall can lead out the liquid while preventing solid impurities from flowing down the surface of the separation wall, thereby ensuring the separation effect and efficiency. The separated liquid and mucus fall along the drainage wall 17 and gather in the liquid storage layer 18 .

[0026] Furthermore, the separation device 8 also includes a separation cylinder 19, a barrel cover 20, a centrifugal chassis 21, a sewage outlet 22, and a water inlet mechanism 23. The barrel cover 20 is arranged below the separation cylinder 19 and is sealed and slidably connected to the separation cylinder 19. The separation cylinder 19 is arranged to be a conical barrel structure that is narrow at the top and bottom and wide in the middle. The centrifugal chassis 21 is arranged below the separation cylinder 19. The sewage outlet 22 is arranged below the separation cylinder 19 and passes through the centrifugal chassis 21 to be connected to the sewage tank 10 through a pipeline. The water inlet mechanism 23 is arranged inside the separation cylinder 19 and passes through the barrel cover 20 to be connected to the upper inlet of the sub-packaging device 9; The mixed liquid enters the separation cylinder 19 after preliminary filtration, and the separation cylinder 19 is driven to rotate by the centrifugal chassis 21, so that the mixed liquid in the cylinder is rotated and centrifuged. The mucus in the mixed liquid is distributed in the upper layer of the bacterial liquid due to the centrifugal effect, and the solid impurities in the mixed liquid are distributed in the lower layer of the bacterial liquid. The stratified bacterial liquid is discharged into the sub-packaging device 9 by the water inlet mechanism 23, and the upper mucus and the lower solid impurities can be discharged into the sewage tank 10 through the sewage outlet 22.

[0027] Furthermore, the water inlet mechanism 23 further includes a hose 24, a water inlet 25, a chute 26, and a float 27. The hose 24 is connected to the right side of the water inlet 25, and the lower part of the chute 26 is connected to the left side of the water inlet 25. The float 27 is arranged in the chute 26 and can slide freely, and the diameter of the float 27 is larger than the diameter of the inlet at the connection between the water inlet 25 and the chute 26. The water inlet mechanism 23 is suspended in the bacterial liquid in the middle layer by the buoyancy of the float 27. The nitrogen system 3 pressurizes the separation cylinder 19 to allow the bacterial liquid to enter the hose 24 from the water inlet 25. When the bacterial liquid is emptied, the float 27 falls back along the chute 26 to block the water inlet 25, thereby preventing other impurities from being discharged into the subpackaging device 9.

[0028] Furthermore, the density of the float 27 is less than the density of the bacterial liquid after stratification and greater than the density of the upper mucus after stratification; Among them, when the mixed liquid is layered, the float 27 floats in the chute 26 under the action of buoyancy in the bacterial liquid. At the same time, the density of the float 27 is greater than the density of the upper mucus, so that the water inlet mechanism 23 is kept in the bacterial liquid layer, and the water inlet 25 is opened. The nitrogen system 3 can be discharged from the hose 24 after pressurization. When the bacterial liquid is drained, the float 27 falls and blocks the water inlet 25 to complete the separation. In this way, the bacterial liquid is separated from the mucus and impurities by the wall adhesion effect and centrifugal action of the liquid, so that the filter screen will not be blocked, thereby increasing the filtering and extraction efficiency of the bacterial liquid.

[0029] Furthermore, the dispensing device 9 further includes a liquid storage box 28, an electric valve b29, a contact switch 30, a slider 31, a needle 32, and a bottle mouth limiting mechanism. The solenoid valve b is arranged below the liquid storage box 28, the contact switch 30 is arranged on the left side of the electric valve b29 and controls the opening and closing of the electric valve b29, the slider 31 is arranged below the contact switch 30, the needle 32 is slidably sleeved on the inner side of the slider 31, and the bottle mouth limiting mechanism is arranged below the slider 31 and is slidably connected to the slider 31; Among them, after the separated bacterial liquid enters the liquid storage box 28, the operator aligns the mark of the vacuum filling bottle 5 and inserts it into the bottle mouth limit device, rotates the bottle body counterclockwise, inserts the needle 32 into the bottle, and the top of the bottle presses against the slider 31, triggering the contact switch 30 upward, opening the electric valve b29, and loading the bacterial liquid into the vacuum filling bottle 5 through the needle 32.

[0030] Furthermore, the bottle mouth limiting mechanism further includes a limiting block 33, an access port 34, and a mark. The limiting blocks 33 are respectively arranged at the inner lower part of the bottle mouth limiting mechanism, and the gap between the limiting blocks 33 forms the access port 34. The limiting blocks 33 are provided with an inclined surface to divide the limiting blocks 33 into an upper layer and a lower layer, and the mark (not shown in the figure) is arranged on the right side of the access port 34. Among them, the vacuum filling bottle 5 enters from the access port 34, and the block 35 is rotated into the upper part of the lower layer of the limit block 33. At this time, the needle 32 is inserted into the bottle. At this time, the slider 31 moves up but does not trigger the contact switch 30. Then the bottle body continues to rotate, so that the block 35 moves up along the inclined surface of the limit block 33 and comes to the upper layer of the limit block 33, and at the same time triggers the contact switch 30, opens the electric valve b29, and discharges the bacterial liquid in the liquid storage box 28 into the vacuum filling bottle 5. When the bacterial liquid is almost full, the bottle body is rotated in the direction to make the block 35 return to the lower layer of the limit block 33. At this time, the contact switch 30 is disconnected, the electric valve b29 is closed, and the vacuum filling bottle 5 continues to suck the remaining bacterial liquid in the needle 32 into the bottle to prevent the residual bacterial liquid and gas from escaping to the outside to cause pollution during the filling.

[0031] Furthermore, the vacuum dispensing bottle 5 is provided with stoppers 35 on both sides, and the outer side of the stoppers 35 is provided with a mark of the same color as the bottle mouth limiting mechanism (not shown in the figure); Among them, the block 35 can make the vacuum filling bottle 5 stuck on the limit block 33, and the linkage slider 31 triggers the electric valve b29 to open and close.

[0032] In summary, the device includes a box body 1, a feeding device 2, a raw material processing system, a nitrogen system 3, a water supply system 4, and a vacuum filling bottle 5. A stirring device 6, a filtering device 7, a separating device 8, a filling device 9, and a sewage tank 10 in the raw material processing system are provided, and the device is connected to the nitrogen system 3 and the water supply system 4. While filtering the mixed liquid, the impurities filtered in each step can be discharged into the sewage tank 10 respectively, and the device is automatically cleaned to reduce the operation process. At the same time, the device is isolated from the external environment during the treatment process to prevent the external environment from being polluted during the extraction process; the filtering device 7 and the separating device 8 are used to filter and extract the bacterial liquid separately, and the bacterial liquid is separated from the mucus and impurities by the wall adhesion effect and centrifugal action of the liquid, so that the filter screen will not be blocked, and the filtering and extraction efficiency of the bacterial liquid is increased; the filling device 9 is provided, and the extracted bacterial liquid can be distributed and filled into bacterial liquid bottles of different specifications, and the bacterial liquid in the front end of the filling device 9 can be automatically cleaned to prevent the residual bacterial liquid and gas from escaping to the outside world during the filling to cause pollution. Example 2

[0033] like Figures 1 to 7 As shown: Based on the above technical solution, the present invention provides a method for preparing intestinal microecological cleaning bacteria, which specifically comprises the following steps: S1: Put the fresh feces sample into the feeding device 2, then close the valve of the feeding device 2, and introduce nitrogen into the feeding device 2 through the nitrogen system 3 to pass the air from the feeding device 2 through the stirring device 6 and the separation device 8, and finally enter the exhaust pipe above the sewage tank 10 for filtration and discharge, and then the water supply system 4 sprays sterile saline into the feeding device 2 to bring the feces into the stirring device 6; S2: The stool sample and the sterile physiological saline are stirred and mixed in the stirring device 6 to form a mixed solution; S3: After the mixed liquid is fully stirred, it is slowly discharged downwards into the filtering device 7 by the stirring device 6. As the mixed liquid gradually fills the middle of the separation wall, most of the solid matter in the mixed liquid is deposited at the bottom of the separation wall. Due to the wall adhesion effect of the liquid, the overflowed liquid and mucus flow downwards along the surface of the separation wall through the drainage wall 17 and collect into the liquid storage layer 18 to complete the preliminary filtration. At the same time, the driving motor 14 drives the stirring disk 11 to rotate slowly to accelerate this process. S4: the mixed liquid after preliminary filtration enters the separation cylinder 19 from the liquid storage layer 18. In the separation cylinder 19, the centrifugal chassis 21 drives the mixed liquid in the separation cylinder 19 to rotate and centrifuge, and the mixed liquid is separated into an upper layer composed of mucus, a middle layer composed of bacterial liquid, and a lower layer composed of impurities; S5: After the mixed liquid is centrifuged and layered in the separation device 8 and allowed to stand, the nitrogen system 3 introduces nitrogen into the separation cylinder 19, and the bacterial liquid in the middle layer enters the liquid storage tank 28 of the subpackaging device 9 through the water inlet mechanism 23 floating in the bacterial liquid layer, thereby completing the extraction of the bacterial liquid; S6: Use a suitable vacuum sub-filling bottle 5, align the mark on the stopper 35 with the mark on the bottle mouth limit mechanism, insert it upward and rotate it counterclockwise, so that the needle 32 is inserted into the vacuum sub-filling bottle 5 and presses against the slider 31, open the electric valve b29 through the contact switch 30, so that the negative pressure in the vacuum sub-filling bottle 5 sucks the bacterial liquid into the sub-filling bottle, and when it is almost full, turn the vacuum sub-filling bottle 5 clockwise to close the electric valve b29, and after the remaining vacuum pressure in the sub-filling bottle sucks out the remaining bacterial liquid in the needle 32, remove the vacuum sub-filling bottle 5 to complete the extraction; S7: After the bacterial liquid is extracted, the device is rinsed clean with sterile saline provided by the water supply system 4, and the sewage flushed out is stored in the sewage tank 10 for treatment. The nitrogen system 3 also provides nitrogen to maintain the oxygen-free environment in the device, and the gas is discharged from the top of the sewage tank 10 after filtering.

[0034] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0035] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for preparing intestinal microecological washing bacteria, comprising a housing (1), a feeding device (2), a raw material processing system, a nitrogen system (3), a water supply system (4), a vacuum filling bottle (5), and a control system, wherein the feeding device (2) is arranged inside the upper left side of the housing (1), the raw material processing system is arranged below the feeding device (2), the nitrogen system (3) is arranged inside the upper right side of the housing (1), and the water supply system (4) is arranged on the left side of the nitrogen system (3). The nitrogen system (3) for providing an oxygen-free environment for the device and the water supply system (4) for providing sterile physiological saline for cleaning are respectively connected to the feeding device (2) and the raw material processing system through pipelines, the vacuum filling bottle (5) is arranged below the outlet of the raw material processing system, and the control system is respectively connected to the housing (1), the feeding device (2), the raw material processing system, the nitrogen system (3), the water supply system (4), and the vacuum filling bottle (5) by telecommunication, characterized in that: The raw material processing system further comprises a stirring device (6), a filtering device (7), a separating device (8), a filling device (9), and a sewage tank (10). The stirring device (6) is arranged on the right side of the feeding device (2) and is connected to the feeding device (2) via a pipeline. The filtering device (7) is arranged below the stirring device (6) and is directly opposite to the pipeline outlet below the stirring device (6). The separating device (8) is arranged on the right side below the filtering device (7) and is connected to the filtering device (7) via a pipeline. The filling device (9) is arranged on the right side of the separating device (8) and an upper inlet of the filling device (9) is connected to an upper outlet of the separating device (8) via a pipeline. The sewage tank (10) is arranged below the filtering device (7) and is connected to the filtering device (7) and the separating device (8) via pipelines.

2. A device for preparing intestinal microecological cleaning bacteria according to claim 1, characterized in that: The filtering device (7) further comprises a separation wall, a stirring plate (11), a rack (12), a gear (13), a driving motor (14), and an electric valve a (15); the stirring plate (11) is arranged in the middle of the separation wall and is sealed and slidably connected to the separation wall; the rack (12) is arranged below the stirring plate (11); the gear (13) is arranged on the left side of the rack (12) and meshes with the rack (12); the output end of the driving motor (14) is connected to the bottom of the rack (12); the electric valve a (15) is arranged below the stirring plate (11) and is connected to a pipe in the middle of the stirring plate (11); and the bottom of the electric valve a (15) is connected to a sewage tank (10) through a pipe.

3. A device for preparing intestinal microecological cleaning bacteria according to claim 2, characterized in that: The separation wall further comprises a separation layer (16), a drainage wall (17), and a liquid storage layer (18); the separation layer (16) having an outwardly expanding annular shape and a rough surface is arranged above the drainage wall (17); the liquid storage layer (18) is arranged below the drainage wall (17); and an outlet is arranged at the lower right side of the liquid storage layer (18) and is connected to an inlet above the separation device (8) through a pipeline.

4. The device for preparing intestinal microecological cleaning bacteria according to claim 1, characterized in that: The separation device (8) further comprises a separation barrel (19), a barrel cover (20), a centrifugal chassis (21), a sewage outlet (22), and a water inlet mechanism (23); the barrel cover (20) is arranged below the separation barrel (19) and is sealingly and slidably connected to the separation barrel (19); the separation barrel (19) is arranged as a conical barrel structure which is narrow at the top and bottom and wide in the middle; the centrifugal chassis (21) is arranged below the separation barrel (19); the sewage outlet (22) is arranged below the separation barrel (19) and is connected to the sewage tank (10) through a pipeline passing through the centrifugal chassis (21); and the water inlet mechanism (23) is arranged inside the separation barrel (19) and is connected to the upper inlet of the sub-packaging device (9) through the barrel cover (20).

5. A device for preparing intestinal microecological cleaning bacteria according to claim 4, characterized in that: The water inlet mechanism (23) further comprises a hose (24), a water inlet (25), a chute (26), and a float (27); the hose (24) is connected to the right side of the water inlet (25); the bottom of the chute (26) is connected to the left side of the water inlet (25); the float (27) is slidably mounted inside the chute (26); and the diameter of the float (27) is larger than the diameter of the inlet at the connection between the water inlet (25) and the chute (26).

6. A device for preparing intestinal microecological cleaning bacteria according to claim 5, characterized in that: The density of the float (27) is less than the density of the bacterial liquid after stratification and greater than the density of the upper mucus after stratification.

7. The device for preparing intestinal microecological cleaning bacteria according to claim 1, characterized in that: The dispensing device (9) further comprises a liquid storage box (28), an electric valve b (29), a contact switch (30), a slider (31), a needle (32), and a bottle mouth limiting mechanism, wherein the electric valve b (29) is arranged below the liquid storage box (28), the contact switch (30) is arranged on the left side of the electric valve b (29) and controls the opening and closing of the electric valve b (29), the slider (31) is arranged below the contact switch (30), the needle (32) is slidably sleeved on the inner side of the slider (31), and the bottle mouth limiting mechanism is slidably sleeved below the slider (31).

8. The device for preparing intestinal microecological cleaning bacteria according to claim 7, characterized in that: The bottle mouth limiting mechanism further comprises a limiting block (33), an access port (34), and a mark. The limiting blocks (33) are respectively arranged at the front and rear sides of the lower inner side of the bottle mouth limiting mechanism. The gap between the limiting blocks (33) constitutes the access port (34). The limiting blocks (33) are provided with an inclined surface to divide the limiting blocks (33) into an upper layer and a lower layer. The mark is arranged on the right side of the access port (34).

9. The device for preparing intestinal microecological cleaning bacteria according to claim 1, characterized in that: The vacuum dispensing bottle (5) is further provided with stoppers (35) on the left and right sides, and a mark having the same color as that on the bottle mouth limiting mechanism is provided on the outside of the stoppers (35).

10. A method for preparing intestinal microecological cleaning bacteria according to any one of claims 1 to 9, characterized in that: The specific steps include: S1: A fresh stool sample is placed in a feeding device (2), and a nitrogen system (3) introduces nitrogen into the feeding device (2) to ensure an anaerobic environment in the feeding device (2). Then, a water supply system (4) sprays sterile saline to bring the stool into a stirring device (6); S2: The stool sample and sterile physiological saline are stirred and mixed in a stirring device (6) to form a mixed solution; S3: After the mixed liquid is fully stirred, it is slowly discharged downward into the filtering device (7). Due to the wall adhesion effect of the liquid, the overflowed liquid and mucus are collected along the surface of the separation layer (16) to the liquid storage layer (18), and the solid impurities are precipitated to the bottom of the separation layer (16). At the same time, the driving motor (14) drives the stirring plate (11) to rotate slowly to accelerate the separation process; S4: the mixed liquid after preliminary filtration enters the separation cylinder (19) from the liquid storage layer (18), and the centrifugal bottom plate (21) drives the mixed liquid in the separation cylinder (19) to rotate and centrifuge; S5: The nitrogen system (3) introduces nitrogen into the separation cylinder (19) for pressurization, and presses the bacterial liquid in the middle layer into the liquid storage box (28) of the subpackaging device (9) through the water inlet mechanism (23) floating in the bacterial liquid layer, thereby completing the extraction of the bacterial liquid; S6: Use a suitable vacuum dispensing bottle (5), align the mark on the stopper (35) with the mark on the bottle mouth limit mechanism, insert it upward and rotate it counterclockwise, so that the needle (32) is inserted into the vacuum dispensing bottle (5) to absorb the bacterial solution. When it is almost full, turn the vacuum dispensing bottle (5) clockwise, and after the remaining vacuum pressure sucks out the remaining bacterial solution in the needle (32), remove the vacuum dispensing bottle (5) to complete the extraction; S7: After the bacterial liquid is extracted, sterile physiological saline is provided through the water supply system (4) to rinse the device clean, and the wastewater from the rinse is stored in the wastewater tank (10) to be processed. The nitrogen system (3) also provides nitrogen to maintain the pressure balance in the device, and the gas is discharged from the top of the wastewater tank (10) after being filtered.

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