Constant-temperature incubation device and in-vitro detection instrument

Abstract

The invention provides a constant-temperature incubation device and an in-vitro detection instrument, and relates to the technical field of medical detection instruments, the constant-temperature incubation device comprises an upper cover plate, an incubation disc, a heating piece, a base and a driving assembly which are sequentially arranged, a containing cavity is formed between the upper cover plate and the base, the incubation disc and the heating piece are located in the containing cavity, and a temperature sensor and a reagent piece are arranged on the incubation disc; the driving assembly drives the incubation disc to rotate, the temperature sensor performs temperature detection feedback on the incubation disc, and the heating sheet heats the incubation disc according to the temperature detection feedback. The incubation plate is used for carrying the reagent piece to rotate, so that the scraping of the reagent piece can be avoided, and the movement stability of the reagent piece is ensured; the heating sheet serving as a heat source is directly connected with the incubation plate for heating, so that the energy loss caused by excessive heat conduction is reduced, and the startup temperature rise time is shortened. The requirement for high-precision temperature control is met, the stability of the operation state of the reagent piece is also ensured, and the reagent piece is designed in an extremely small structure size so as to be conveniently applied to a high-integration-level instrument with a limited internal space.

Description

Technical Field

[0001] The present application relates to the technical field of medical detection instruments, and in particular to a constant temperature incubation device and an in vitro detection instrument. Background Art

[0002] In the sample analysis instrument, after the reagent sheet is dripped, it must enter the incubation area for incubation and testing of the reagent sheet after incubation. In addition to providing the space required for the incubation of the reagent sheet, the incubation area also provides a precise temperature control environment. At present, the constant temperature incubation methods used by the fully automatic immunochromatographic analyzer mainly include air bath and solid direct heating (solid direct contact constant temperature). Among them, the advantage of the solid direct heating constant temperature method is low cost, and the reaction solution in the incubation area is heated quickly, evenly and stably.

[0003] However, the existing systems using solid direct heating reaction plates have the following main problems: First, the temperature control part of the reaction plate of the existing fluorescent immunochromatographic analyzer is to heat the shell of the reaction plate, which has low heating efficiency and high power consumption. In addition, the temperature sensor does not directly monitor and feedback the temperature of the incubation plate, which is bound to be different from the temperature control target.

[0004] Secondly, the base and the turntable can be separated so that the base does not need to rotate, and the heating plate can be directly attached to the base, avoiding the appearance of an air layer between the base and the heating plate, so that the heating plate can directly heat the reagent sheet through the base, which can improve the heating effect. However, since the base of the reagent sheet is separated from the movement, it must rely on the chassis for heating, and it must also rotate to meet the requirements of automatic loading and unloading and testing. Therefore, there will inevitably be scratches between the reagent sheet and the chassis, and the stability of the reagent sheet cannot be guaranteed, which in turn affects the incubation effect. Summary of the invention

[0005] The purpose of the embodiments of the present application is to provide a constant temperature incubation device and an in vitro detection instrument, which not only ensures the requirements of high-precision temperature control, but also ensures the stability of the operating state of the reagent sheet.

[0006] According to one aspect of an embodiment of the present application, a constant temperature incubation device is provided, comprising an upper cover plate, an incubation tray, a heating plate, a base and a driving assembly which are arranged in sequence, wherein a receiving cavity is formed between the upper cover plate and the base, the incubation tray and the heating plate are located in the receiving cavity, a temperature sensor and a reagent sheet are arranged on the incubation tray, the driving assembly drives the incubation tray to rotate, the temperature sensor performs temperature detection and feedback on the incubation tray, and the heating plate heats the incubation tray according to the temperature detection feedback.

[0007] Optionally, the driving assembly includes a motor, a driving shaft connected to the motor, and a driving disk connected to the driving shaft, the driving disk is connected to the incubation disk, and the motor is fixed to the base via a fixing component.

[0008] Optionally, it further comprises a positioning component, wherein the positioning component is used to generate a positioning signal when it is in a preset position to initially position the incubation tray;

[0009] The positioning component includes a movable baffle and a detection device, wherein the detection device is fixed to the base, and the movable baffle is fixed to the incubation tray to rotate synchronously with the incubation tray.

[0010] Optionally, a detection component is further included, and notches are respectively provided on the base and the incubation tray, and the detection component detects the reagent sheet through the notches.

[0011] Optionally, it also includes a conductive slip ring, which is connected to the fixed component and is concentrically arranged with the motor shaft of the motor. The conductive slip ring includes an outer ring and an inner ring sleeved in the outer ring, a cable is arranged on the inner ring, and the inner ring is fixed to the drive shaft so that the inner ring, the cable and the incubation plate rotate synchronously.

[0012] Optionally, the upper cover is provided with a tablet pushing port, and the reagent tablet is pushed through the tablet pushing port. The corresponding position of the base is provided with an inlet and outlet for the reagent tablet to enter and exit the incubation tray.

[0013] Optionally, there are multiple temperature sensors, and the multiple temperature sensors are respectively located on the incubation tray and evenly distributed along the central circumference of the incubation tray to divide the incubation tray into multiple areas for temperature detection.

[0014] Optionally, a heat preservation component is provided on a side of the base facing the driving assembly, and / or a heat preservation component is provided on a side of the upper cover away from the incubation tray.

[0015] On the other hand, an embodiment of the present application provides an in vitro detection instrument, including: a frame, in which a sampling module, a sample transfer module, a reagent sheet transport module, a detection module, a liquid circuit module, a collecting device and the above-mentioned constant temperature incubation device are arranged, the sample transfer module draws the sample in the sampling module into the liquid circuit module for pre-reaction treatment, and then transfers the treated sample to the reagent sheet transfer module, the reagent sheet that has completed dripping is transported to the constant temperature incubation device for constant temperature incubation, the reagent sheet that has completed incubation is transported to the detection module for detection, and the sample tubes and reagent sheets that have completed detection can be centrally processed by the collecting device.

[0016] Optionally, a detection slot is provided on the upper cover plate, the detection slot is connected to the incubation tray, and the detection module performs optical detection on the reagent sheet on the incubation tray through the detection slot.

[0017] The constant temperature incubation device and in vitro detection instrument provided by the embodiment of the present application, the driving component drives the incubation disk to rotate, the heating plate directly heats the incubation disk, and the temperature sensor on the upper surface of the incubation disk monitors and feedbacks the temperature of the incubation disk. When the temperature sensor monitors that the temperature of the incubation disk reaches the target temperature, the heating plate stops working, and then the constant temperature maintenance action of the heating plate is started and stopped according to the heating power and the actual working conditions, so that the incubation disk always maintains the target temperature required for the incubation of the reagent sheet to achieve the incubation function of the reagent sheet. The use of the incubation disk to support the reagent sheet for rotational movement can avoid the scratching of the reagent sheet, ensure the movement stability of the reagent sheet, and effectively ensure the incubation effect; the heating plate as a heat source is directly connected to the incubation disk for heating, reducing the energy loss caused by excessive heat conduction and reducing the startup temperature rise time. The constant temperature incubation device provided by the embodiment of the present application ensures the requirements of high-precision temperature control, and also ensures the stability of the operating state of the reagent sheet, and is designed with extremely small structural dimensions to facilitate application to high-integration instruments with limited internal space. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required for use in the embodiments of the present application will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0019] Figure 1 This is one of the structural schematic diagrams of the constant temperature incubation device provided in this embodiment;

[0020] Figure 2 This is the second structural schematic diagram of the constant temperature incubation device provided in this embodiment;

[0021] Figure 3 This is the third structural schematic diagram of the constant temperature incubation device provided in this embodiment;

[0022] Figure 4 This is the fourth structural schematic diagram of the constant temperature incubation device provided in this embodiment;

[0023] Figure 5 This is one of the schematic diagrams of the structure of the in vitro detection instrument provided in this embodiment;

[0024] Figure 6 This is the second structural schematic diagram of the in vitro detection instrument provided in this embodiment.

[0025] Icon: 1-frame; 2-injection module; 3-sample transfer module; 4-reagent sheet transport module; 5-detection module; 6-constant temperature incubation device; 61-sheet push port; 62-optical coupler; 63-detection slot; 601-pad; 602-insulation component; 603-upper cover; 604-incubation plate; 604a-card slot; 604b-installation slot; 605-heating plate; 606-base; 606a-accommodating cavity; 606b-inlet and outlet; 606c-guide structure; 607-drive plate; 608-insulation component; 609-drive shaft; 610-conductive slip ring; 611-fixed component; 612-motor; 613-detection component; 614-positioning component; 615-temperature sensor; 7-reagent sheet; 8-liquid path module. DETAILED DESCRIPTION

[0026] The technical solutions in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application.

[0027] In the description of this application, it should be noted that the terms "inside", "outside", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the application is usually placed when in use, which is only for the convenience of describing this application and simplifying the description, and does not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on this application. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance.

[0028] It should also be noted that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a direct connection, or an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0029] Please refer to Figure 1 , Figure 2 As shown, an embodiment of the present application provides a constant temperature incubation device 6, comprising: an upper cover plate 603, an incubation tray 604, a heating plate 605, a base 606 and a driving component arranged in sequence from top to bottom, a receiving cavity 606a is formed between the upper cover plate 603 and the base 606, the incubation tray 604 and the heating plate 605 are located in the receiving cavity 606a, a temperature sensor 615 and a reagent sheet 7 are arranged on the incubation tray 604, the driving component drives the incubation tray 604 to rotate, the temperature sensor 615 performs temperature detection and feedback on the incubation tray 604, and the heating plate 605 heats the incubation tray 604 according to the temperature detection feedback.

[0030] The driving assembly is used to provide driving force, and the driving assembly passes through the base 606 and the heating plate 605, and is connected to the incubation tray 604, so as to drive the incubation tray 604 to rotate. In order to avoid the problems of scratching and unstable movement between the reagent sheet 7 and the chassis in the prior art, the present application combines the rotating motion bearing surface of the reagent sheet 7 with the driving tray 607, and the incubation tray 604 carries the reagent sheet 7 for rotational movement.

[0031] In addition, the heating plate 605 is designed to be pasted on the bottom surface of the incubation tray 604 to directly heat the temperature control target (incubation tray 604). The heating plate 605 can be designed in blocks or as a whole to meet the temperature control requirements of different scenarios. This effectively reduces excessive heat chain transfer between the heating plate 605 and the heating target (incubation tray 604), improves the heating effect, and reduces heating power consumption.

[0032] When the incubation tray 604 is temperature controlled, a heating plate 605 is installed on the bottom surface of the incubation tray 604, a temperature sensor 615 is installed on the incubation tray 604, and a temperature protector is installed on the upper surface of the upper cover plate 603. During operation, the heating plate 605 directly heats the incubation tray 604, and the temperature sensor 615 on the upper surface of the incubation tray 604 monitors and provides feedback on the temperature of the incubation tray 604. When the temperature sensor 615 monitors that the temperature of the incubation tray 604 reaches the target temperature, the heating plate 605 stops working, and then the heating plate 605 is started and stopped according to the heating power and the actual working conditions to maintain the constant temperature, so that the incubation tray 604 always maintains the target temperature required for the incubation of the reagent sheet 7.

[0033] Furthermore, the temperature protector installed on the upper cover plate 603 can detect the temperature state of the upper cover plate 603. There is a certain temperature difference between the temperature of the upper cover plate 603 and the temperature of the incubation tray 604. When the temperature of the incubation tray 604 is overheated and transmitted to the upper cover plate 603 and detected by the temperature protector, the temperature protector can disconnect the circuit of the heating plate 605 to implement temperature overheat protection, which can prevent the situation that, for example, the temperature sensor 615 fails and causes the instrument to heat up infinitely, thereby causing damage to components.

[0034] The upper cover 603 and the base 606 are connected by positioning pins and fasteners, and a receiving cavity 606a is formed between the upper cover 603 and the base 606, so that the incubation tray 604 and the heating plate 605 are placed in the receiving cavity 606a. The receiving cavity 606a can meet the premise that the reagent sheet 7, the incubation tray 604 and the heating plate 605 operate normally, and adopts a smaller size design to provide a relatively constant temperature space, avoiding energy waste and temperature fluctuations caused by too large a cavity or too much exposure.

[0035] like Figure 1 As shown, the upper cover plate 603 is provided with a sheet pushing port 61, and the reagent sheet 7 is pushed through the sheet pushing port 61. Figure 4As shown, an inlet and outlet 606 b is provided at a corresponding position of the base 606 for the reagent sheet 7 to enter and exit the incubation tray 604 .

[0036] For loading and unloading the reagent slice 7, a slice pushing port 61 is provided on the upper cover plate 603. In order to prevent too many slots from affecting the heat preservation effect, the slice loading and unloading are unified at one position, and an inlet and outlet 606b is provided at the corresponding position of the base 606. The upper cover plate 603 has a slice pushing port 61 at the slice loading and unloading position for slice pushing operation; the base 606 has an inlet and outlet 606b on the side at the corresponding position for the reagent slice 7 to enter and exit the incubation tray 604.

[0037] After the reagent sheet 7 enters the incubation tray 604, it directly falls into the slot 604a of the incubation tray 604. The notch of the slot 604a of the incubation tray 604 is chamfered to facilitate the pushing / falling of the reagent sheet 7. The non-centering design of the slot 604a of the incubation tray 604 can provide useful help to prevent the reagent sheet 7 from being thrown out of the incubation tray 604 during operation, that is, when the thrust direction and the rotation radial direction are in an acute angle state, a centripetal thrust can be generated, which can prevent the reagent sheet 7 from being thrown out under the action of centrifugal force during rotation to a certain extent.

[0038] like Figure 2 As shown, a guide structure 606c is further provided at the inlet and outlet 606b of the base 606 , and the guide structure 606c can gather the reagent sheet 7 to ensure the consistency of the radial position of the reagent sheet 7 when testing on the incubation plate 604 .

[0039] In summary, in the constant temperature incubation device 6 provided in the embodiment of the present application, the driving component drives the incubation disk 604 to rotate, the heating plate 605 directly heats the incubation disk 604, and the temperature sensor 615 on the upper surface of the incubation disk 604 monitors and provides feedback on the temperature of the incubation disk 604. When the temperature sensor 615 monitors that the temperature of the incubation disk 604 reaches the target temperature, the heating plate 605 stops working, and then the constant temperature maintenance action of the heating plate 605 is started and stopped according to the heating power and the actual working conditions, so that the incubation disk 604 always maintains the target temperature required for the incubation of the reagent sheet 7, so as to realize the incubation function of the reagent sheet 7. The incubation tray 604 is used to carry the reagent sheet 7 for rotational motion, which can avoid the scratching of the reagent sheet 7, ensure the movement stability of the reagent sheet 7, and effectively ensure the incubation effect; the heating sheet 605 as a heat source is directly connected to the incubation tray 604 for heating, reducing the energy loss caused by excessive heat conduction and reducing the startup temperature rise time; the temperature sensor 615 is directly connected to the incubation tray 604 to avoid the temperature difference fluctuation and temperature control hysteresis or overshoot caused by external fluctuations during indirect feedback (i.e., the temperature of the temperature control target and the temperature sensor 615 detect the object temperature, due to heat conduction There is a certain time lag or external interference fluctuation); it is also provided by a closed accommodating chamber 606a to provide a relatively constant temperature space to avoid energy waste and temperature fluctuations. The constant temperature incubation device 6 provided in the embodiment of the present application ensures the requirements of high-precision temperature control, and also ensures the stability of the operating state of the reagent sheet 7, and is designed with extremely small structural dimensions to facilitate application to high-integration instruments with limited internal space.

[0040] Among them, Figure 4 As shown, the temperature sensor 615 is embedded in a specific installation groove 604b on the upper surface of the incubation tray 604 by gluing. There are multiple temperature sensors 615, and the multiple temperature sensors 615 are respectively located on the incubation tray 604 and evenly distributed along the central circumference of the incubation tray 604 to divide the incubation tray 604 into multiple areas for temperature detection.

[0041] The temperature sensor 615 is designed to be installed in a specially designed installation groove 604b on the incubation tray 604. For example, the installation groove 604b is a strip-shaped groove, and the temperature sensor 615 is located in the strip-shaped installation groove 604b. The entire incubation tray 604 can be installed with no less than one temperature sensor 615 to monitor the temperature of the incubation tray 604. When multiple temperature sensors 615 are installed on the incubation tray 604, the regional temperature detection of the incubation tray 604 can be performed. When the design of the block-shaped heating plate 605 is matched, the temperature of a certain area of ​​the incubation tray 604 can be accurately controlled.

[0042] For the drive components, Figure 2As shown, it includes a motor 612, a driving shaft 609 connected to the motor 612, and a driving disk 607 connected to the driving shaft 609. The driving disk 607 is connected to the incubation disk 604, and the motor 612 is fixed to the base 606 by a fixing component 611.

[0043] The base 606 is fixed to the column of the fixing part 611 by fasteners, the motor 612 is installed on the fixing part 611, the driving shaft 609 is sleeved on the shaft of the motor 612, and is fixed to the shaft of the motor 612 by fasteners, and the incubation tray 604 is fixed to the driving tray 607 by fasteners. When the motor 612 is working, the shaft of the motor 612 rotates to drive the driving shaft 609 fixed thereto to rotate, and then drives the driving tray 607 fixed to the driving shaft 609 to rotate, and then drives the incubation tray 604 fixed to the driving tray 607 to rotate, forming the rotation drive part of the constant temperature incubation device 6.

[0044] It also includes a positioning component 614, which is used to generate a positioning signal when it is in a preset position to initially position the incubation tray 604; the positioning component 614 includes a movable baffle and a detection device, the detection device is fixed to the base 606, and the movable baffle is fixed to the incubation tray 604 to rotate synchronously with the incubation tray 604.

[0045] The positioning component 614 is mounted on the bottom surface of the base 606 by fasteners, and the movable baffle of the positioning component 614 is fixed to the incubation tray 604 and rotates therewith, and the detection device of the positioning component 614 is fixed on the base 606. When the movable baffle and the detection device are in the preset position, an electrical signal (positioning signal) will be generated for the initial positioning of the incubation tray 604. In the subsequent rotational movement of the incubation tray 604, incremental position feedback can be performed through the step counting and encoding of the motor 612 itself. When the continuous rotation stroke reaches a certain stage, in order to eliminate the cumulative rotation error of the incubation tray 604, the error can be cleared and corrected through initial positioning.

[0046] It also includes a detection component 613. The base 606 and the incubation tray 604 are respectively provided with notches, and the detection component 613 detects the reagent sheet 7 through the notches.

[0047] The detection component 613 is also installed on the bottom surface of the base 606 by fasteners. The detection component 613 can be used to judge the reagent sheet 7. When the incubation tray 604 is in the film loading position / unloading position, the detection component 613 can judge the reagent sheet 7 through the slot of the base 606 and the slot in the incubation position of the incubation tray 604 to judge whether the reagent sheet 7 is in the corresponding position.

[0048] Furthermore, when the incubation tray 604 needs to be fed with a film, the reagent film 7 in the corresponding incubation position of the incubation tray 604 must be emptied. If the detection component 613 detects that there is a reagent film 7 in the incubation position, the film unloading operation must be performed first, and after the film feeding operation is completed, the detection component 613 generates a corresponding electrical signal to feedback that the film feeding is successful. When the incubation tray 604 completes the film unloading operation, the detection component 613 can generate a corresponding electrical signal to feedback that the film unloading is successful.

[0049] The constant temperature incubation device 6 also includes a conductive slip ring 610, which is connected to the fixed component 611 and is concentrically arranged with the motor shaft of the motor 612. The conductive slip ring 610 includes an outer ring and an inner ring sleeved in the outer ring. The fixed end of the outer ring is mounted on the fixed component 611 by screws and is kept concentric with the motor shaft. A cable is arranged on the inner ring, and the inner ring is fixed to the drive shaft 609 by fasteners so that the inner ring, the cable and the incubation plate 604 rotate synchronously.

[0050] By setting up a conductive slip ring 610, the problem of wire winding during continuous rotation of the incubation disk 604 can be solved. The wires at both ends of the conductive slip ring 610 are constructed as an inner ring and an outer ring. The inner ring and the outer ring can rotate independently. During rotation, a special contact structure can ensure the stability of the line within a certain lifespan, ensuring that the line is stable when the driving disk 607 rotates continuously without winding.

[0051] A cushion block 601 is also provided on the upper cover plate 603 , and the cushion block 601 is mounted on the incubation upper cover plate 603 via fasteners.

[0052] A heat preservation component 608 is disposed on a side of the base 606 facing the driving assembly, and / or a heat preservation component 602 is disposed on a side of the upper cover 603 away from the incubation tray 604 .

[0053] The heat-insulating component 608 is pasted and installed on the bottom surface of the base 606, and the heat-insulating component 602 is pasted and installed on the incubation upper cover 603. The heat-insulating component can generally be heat-insulating cotton.

[0054] The wire harness of the heating plate 605 passes through the incubation tray 604 from bottom to top, and the wire harness of the rotating end of the conductive slip ring 610 also passes through the insulation 608, the base 606, the heating plate 605, and the incubation tray 604 from bottom to top through the notch to reach the upper surface of the incubation tray 604, so as to realize the conduction between the wire harness of the conductive slip ring 610 and the circuits of the heating plate 605 and the temperature sensor 615. The raised part of the connecting wire harness is constrained by the pad 601 to ensure that the wire harness does not interfere when the device rotates, and at the same time avoids the excessive increase of the accommodating cavity 606a to meet the movement requirements of the wire harness.

[0055] The constant temperature incubation device 6 of the present application has an overall thickened incubation tray 604 and a weight reduction in the non-functional area, which ensures the overall rigidity of the incubation tray 604 while meeting the moment of inertia requirements, and is further conducive to energy saving in conjunction with a smaller incubation cavity design.

[0056] On this basis, please refer to Figure 5 and Figure 6 As shown, an embodiment of the present application also discloses an in vitro detection instrument, including a frame 1, in which are arranged an injection module 2, a sample transfer module 3, a reagent sheet transport module 4, a detection module 5, a liquid circuit module 8, a collecting device and a constant temperature incubation device 6 as any one of the above items. The sample transfer module 3 absorbs the sample in the injection module 2 into the liquid circuit module 8 for pre-reaction treatment, and then transfers the treated sample to the reagent sheet 7 transfer module. The reagent sheet 7 that has completed dripping is transported to the constant temperature incubation device 6 for constant temperature incubation. The reagent sheet 7 that has completed incubation is transported to the detection module 5 for detection. The sample tubes and reagent sheets 7 that have completed detection can be centrally processed by the collecting device.

[0057] like Figure 6 As shown, the incubation tray 604 rotates clockwise to drive the reagent sheet 7 to rotate together. If there is a certain position deviation of the reagent sheet 7 in the radial direction, the reagent sheet 7 can be gathered by the guide structure 606c of the base 606 to ensure the consistency of the radial position of the reagent sheet 7 when testing on the incubation tray 604. The temperature sensor 615 can provide temperature detection feedback to the incubation tray 604.

[0058] The in vitro detection instrument of the present application compresses the incubation space as much as possible to reduce the temperature rise time, ensure the constant temperature control effect, and reduce energy waste, while ensuring that the reagent sheet 7 does not interfere with the operation.

[0059] For the detection of the reagent sheet 7, Figure 3 As shown, a detection slot 63 is left on the upper cover plate 603 , and the detection slot 63 is connected to the incubation tray 604 . The detection module 5 performs optical detection on the reagent sheet 7 on the incubation tray 604 through the detection slot 63 .

[0060] The detection module 5 is mounted on the upper surface of the upper cover 603 and is used to perform optical detection on the incubated reagent sheet 7. In order to stably carry the detection module 5, the upper cover 603 is made of metal material. Due to its good thermal conductivity, a heat preservation member 602 is attached to the upper cover 603 to prevent the upper cover 603 from losing heat to the incubation area. Both the base 606 and the upper cover 603 are provided with protrusions on the outside for connecting the two.

[0061] The constant temperature incubation device 6 can push the reagent sheet 7 in and out through the sheet pushing port 61. Figure 3The optical coupler 62 shown identifies the reagent sheet 7 in the sheet ejection port 61 , and the detection module 5 performs optical detection on the reagent sheet 7 that has completed the incubation reaction through the detection slot 63 .

[0062] The in vitro detection instrument has the same structure and beneficial effects as the constant temperature incubation device 6 in the above embodiment. The structure and beneficial effects of the constant temperature incubation device 6 have been described in detail in the above embodiment, and will not be repeated here.

[0063] The above description is only an embodiment of the present application and is not intended to limit the protection scope of the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A constant temperature incubation device, characterized in that: include: An upper cover plate, an incubation tray, a heating plate, a base and a driving assembly are arranged in sequence, a receiving cavity is formed between the upper cover plate and the base, the incubation tray and the heating plate are located in the receiving cavity, a temperature sensor and a reagent sheet are arranged on the incubation tray, the driving assembly drives the incubation tray to rotate, the temperature sensor performs temperature detection and feedback on the incubation tray, and the heating plate heats the incubation tray according to the temperature detection feedback.

2. The constant temperature incubation device according to claim 1, characterized in that: The driving assembly includes a motor, a driving shaft connected to the motor, and a driving disk connected to the driving shaft. The driving disk is connected to the incubation disk, and the motor is fixed to the base via a fixing component.

3. The constant temperature incubation device according to claim 1, characterized in that: It also includes a positioning component, which is used to generate a positioning signal when it is in a preset position to initially position the incubation tray; The positioning component includes a movable baffle and a detection device, wherein the detection device is fixed to the base, and the movable baffle is fixed to the incubation tray to rotate synchronously with the incubation tray.

4. The constant temperature incubation device according to claim 1, characterized in that: It also includes a detection component, and the base and the incubation tray are respectively provided with notches, and the detection component detects the reagent sheet through the notches.

5. The constant temperature incubation device according to claim 2, characterized in that: It also includes a conductive slip ring, which is connected to the fixed component and is concentrically arranged with the motor shaft of the motor. The conductive slip ring includes an outer ring and an inner ring sleeved in the outer ring. A cable is arranged on the inner ring. The inner ring is fixed to the drive shaft so that the inner ring, the cable and the incubation plate rotate synchronously.

6. The constant temperature incubation device according to claim 1, characterized in that: The upper cover plate is provided with a sheet pushing port, and the reagent sheet is pushed through the sheet pushing port. The corresponding position of the base is provided with an inlet and outlet for the reagent sheet to enter and exit the incubation tray.

7. The constant temperature incubation device according to claim 1, characterized in that: There are multiple temperature sensors, which are respectively located on the incubation tray and evenly distributed along the central circumference of the incubation tray to divide the incubation tray into multiple areas for temperature detection.

8. The constant temperature incubation device according to claim 1, characterized in that: A heat preservation component is arranged on a side of the base facing the driving assembly, and / or a heat preservation component is arranged on a side of the upper cover away from the incubation tray.

9. An in vitro detection instrument, characterized in that: The invention comprises a frame, in which a sampling module, a sample transfer module, a reagent sheet transport module, a detection module, a liquid circuit module, a collecting device and the constant temperature incubation device according to any one of claims 1 to 8 are arranged. The sample transfer module absorbs the sample in the sampling module into the liquid circuit module for pre-reaction treatment, and then transfers the treated sample to the reagent sheet transfer module. The reagent sheet that has completed dripping is transported to the constant temperature incubation device for constant temperature incubation. The reagent sheet that has completed incubation is transported to the detection module for detection. The sample tubes and reagent sheets that have completed detection can be centrally processed by the collecting device.

10. The in vitro detection instrument according to claim 9, characterized in that: The upper cover plate is provided with a detection slot, the detection slot is communicated with the incubation tray, and the detection module performs optical detection on the reagent sheet on the incubation tray through the detection slot.

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