Accurate grinding device of stereotactic injection glass electrode for animal experiment

Abstract

The invention discloses an animal experiment stereotactic injection glass electrode accurate grinding device which comprises a bottom plate, a motor, a grinding piece and a clamping block, the motor and the grinding piece are located at the top of the bottom plate, the clamping block is located at the top of the bottom plate, a fixing block is installed on one side of the top of the bottom plate, a dovetail groove is formed in the top of the fixing block, and a threaded hole is formed in one side face of the fixing block. A fixing plate is installed on one side face of the front-back operation arm, a connecting rod is rotationally installed on the fixing plate, and a movable rotary knob is fixed to the end, close to the fixing plate, of the connecting rod. When the grinding device for the glass electrode is used, the glass electrode can be fixed on a stereotaxic instrument, and the glass electrode can be accurately ground by adjusting the angle and the speed according to the edge condition of the glass electrode in an experiment; the precision of positioning injection can be improved, and the damage to tissues in the injection process is reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of animal experiment instruments, and in particular relates to a precise grinding device for stereotaxic injection glass electrodes in animal experiments. Background Art

[0002] The glass electrode is a membrane electrode made of a glass film that has a potential response to the activity of hydrogen ions. It is a commonly used hydrogen ion indicator electrode. It is usually spherical and is immersed in pure water before use to form a thin swelling layer on the surface. When in use, it and another reference electrode are placed in the solution to be tested to form a battery. The battery potential is directly related to the pH value of the solution. Due to factors such as asymmetric potential and liquid junction potential, the pH value cannot be directly obtained from the battery potential. It should be "calibrated" with a standard buffer solution and the result is obtained according to the definition of pH. The glass electrode is not affected by oxidants, reducing agents and other impurities, has a wide pH measurement range, and is widely used.

[0003] The existing glass microelectrodes are not completely flat on the cut surface and edges after being drawn. This not only increases the mechanical damage to the injection site during microinjection, but also easily leads to needle blockage during positioning injection, that is, tissue fluid accumulates at the tip of the glass electrode, and drugs and reagents cannot smoothly reach the positioned tissue. For brain areas that require precise positioning, this may affect the accuracy of injection positioning, thereby affecting the accuracy of the experiment and the analysis of the results.

[0004] However, there is currently no precise grinding device suitable for positioning injection glass electrodes in animal experiments. The related devices are all for industrial use and cannot adapt well to the needs of small animal experiments.

[0005] The device can not only realize accurate grinding of the angle and position of the glass electrode according to needs, but also improve the accuracy and precision of the injection tissue position when the glass electrode is used. Summary of the invention

[0006] The purpose of the present invention is to provide a precise grinding device for stereotactic injection glass electrodes for animal experiments to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a precise grinding device for stereotaxic injection glass electrodes for animal experiments, comprising a bottom plate, a motor and a grinding sheet located on the top of the bottom plate, and a clamping block located on the top of the bottom plate, a fixed block is installed on one side of the top of the bottom plate, a dovetail groove is provided on the top of the fixed block, a threaded hole is provided on one side of the fixed block, a front and rear operating arm with a trapezoidal side surface is slidably installed on the fixed block through the dovetail groove, a fixed plate is installed on one side of the front and rear operating arms, a connecting rod is rotatably installed on the fixing plate, a moving knob is fixed on one end of the connecting rod close to the fixing plate, and a threaded head is provided on one end of the connecting rod away from the fixing plate;

[0008] A support plate is installed on the top of the bottom plate, a cavity is formed between the support plate and the bottom plate, the motor is located in the cavity formed by the support plate and the bottom plate, and the motor is installed on the bottom wall of the support plate.

[0009] As a preferred embodiment of the present invention, the front and rear operating arms are rotatably mounted at the top of one end away from the fixed plate with a first rotating seat having a groove in the middle, and the bottom circumference of the side wall of the first rotating seat is marked with scale lines for determining the rotation angle of the first rotating seat.

[0010] As a preferred embodiment of the present invention, a tension knob is rotatably mounted on one side surface of the first rotating seat, a second rotating seat is rotatably mounted in the groove of the first rotating seat, and the tension knob acts on the second rotating seat.

[0011] As a preferred embodiment of the present invention, a connecting block is installed on the top of the second rotating seat, and a vertical operating arm is installed on the top of the connecting block.

[0012] As a preferred embodiment of the present invention, a first movable screw is rotatably installed on the top of the vertical operating arm, the vertical operating arm is slidably connected to a common block, and the first movable screw is threadedly connected to the common block.

[0013] As a preferred embodiment of the present invention, a horizontal operating arm is slidably connected to the above-mentioned common block, a second movable screw is rotatably installed on one side surface of the above-mentioned horizontal operating arm, and the above-mentioned second movable screw is threadedly connected to the common block.

[0014] As a preferred embodiment of the present invention, a clamping block for clamping the glass electrode is installed on a side surface of the horizontal operating arm away from the second moving screw, and a fixing knob is rotatably installed on the clamping block.

[0015] As a preferred embodiment of the present invention, a telescopic rod is installed on the side of the top of the base plate away from the fixed block, and a connecting plate is installed on the top of the telescopic rod.

[0016] As a preferred embodiment of the present invention, the connecting plate is slidably mounted with a sliding block through a through groove provided in the connecting plate, and a magnifying glass is sleeved in the middle of the sliding block.

[0017] The technical effects and advantages of the glass electrode grinding device are as follows:

[0018] When using the glass electrode grinding device, the glass electrode is inserted into the clamping block, and the position to be ground is rotated to a position parallel to the grinding sheet by rotating the first rotating seat and the second rotating seat, and then the first moving screw and the second moving screw are rotated to move the glass electrode downward to the top of the grinding sheet. This structure can first rotate the position of the glass electrode to be ground to a position parallel to the grinding sheet according to the actual position to be ground, and then control the glass electrode to move downward to the position of the grinding sheet, so that the position of the glass electrode can be fixed during grinding, thereby improving the grinding accuracy;

[0019] When grinding the glass electrode, open the telescopic rod to drive the connecting plate and the magnifying glass to move to a suitable height, and then slide the position of the magnifying glass through the sliding block to judge the degree of grinding of the glass electrode by the grinding sheet, so as to take out the ground glass electrode in time and reduce the damage caused by excessive grinding; it makes up for the grinding device required for precise grinding of glass electrodes in existing animal experiments or other experiments; this device realizes multi-station, and is a grinding device for glass microelectrode needles with precise magnification and precise adjustment; this equipment belongs to the machinery manufacturing industry, specifically a stereotaxic injection device used in animal brain areas and other tissues, which can be used to meet the needs of precise and quantitative injection of drugs, reagents, etc. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present invention;

[0021] Figure 2 A front view of an embodiment of the present invention;

[0022] Figure 3 This is a schematic diagram of the bottom plate, the fixing block, the support plate and the structure thereon in the embodiment 1 of the present invention;

[0023] Figure 4 For the present invention Figure 1 A schematic diagram of one side of the front and rear operating arms and the upper structure of the embodiment;

[0024] Figure 5 For the present invention Figure 4 An enlarged view of the embodiment at A;

[0025] Figure 6 For the present invention Figure 4 A schematic diagram of the structure of the other side of the embodiment.

[0026] In the figure:

[0027] 101, bottom plate; 102, fixing block; 103, dovetail groove; 104, threaded hole;

[0028] 111. support plate; 112. motor; 113. grinding disc;

[0029] 201, front and rear operating arms; 202, fixing plate; 203, connecting rod; 204, moving knob; 205, threaded head;

[0030] 211, first rotating seat; 212, scale line; 213, tension knob; 214, second rotating seat; 215, connecting block;

[0031] 221, vertical operating arm; 222, first moving screw rod; 223, joint block; 224, horizontal operating arm; 225, second moving screw rod; 226, clamping block; 227, fixing knob;

[0032] 301. telescopic rod; 302. connecting plate; 303. sliding block; 304. magnifying glass. DETAILED DESCRIPTION

[0033] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0034] Please refer to Figure 1-Figure 6 The device shown is a precise grinding device for stereotactic injection glass electrodes for animal experiments, comprising a base plate 101, a motor 112 and a grinding sheet 113 located on the top of the base plate 101, and a clamping block 226 located on the top of the base plate 101. A support plate 111 is installed on the top of the base plate 101, and a cavity is formed between the support plate 111 and the base plate 101. The motor 112 is located in the cavity formed by the support plate 111 and the base plate 101. The motor 112 is installed on the bottom wall of the support plate 111, and the grinding sheet 113 is coaxially installed on the output shaft of the motor 112. The glass electrode is ground by controlling its rotation through the motor 112. A speed regulator switch (the speed regulator switch can be selected as model US-52) is connected between the motor 112 and the grinding sheet 113. The rotation speed of the motor 112 can be controlled by the speed regulator switch, so as to realize different usage modes and perform fast grinding and slow grinding of the glass electrode.

[0035] In order to fix the glass electrode during the grinding process, Figure 1-Figure 2 , Figure 4-Figure 6As shown, a fixing block 102 is installed on one side of the top of the bottom plate 101, a dovetail groove 103 is opened on the top of the fixing block 102, a threaded hole 104 is opened on one side of the fixing block 102, and a front and rear operating arm 201 with a trapezoidal side is slidably installed on the fixing block 102 through the dovetail groove 103, a fixing plate 202 is installed on one side of the front and rear operating arm 201, a connecting rod 203 is rotatably installed on the fixing plate 202, a moving knob 204 is fixed on one end of the connecting rod 203 close to the fixing plate 202, and a moving knob 204 is fixed on the end of the connecting rod 203 away from the fixing plate 202 A threaded head 205 is provided, and the threaded head 205 is threadedly sleeved in the threaded hole 104 of the fixed block 102. The front and rear operating arms 201 are driven to move by moving the knob 204. The front and rear operating arms 201 are rotated away from the top of one end of the fixed plate 202. A first rotating seat 211 with a groove shape in the middle is installed. The bottom circumference of the side wall of the first rotating seat 211 is marked with a scale line 212 for determining the rotation angle of the first rotating seat 211. According to the scale on the scale line 212, the rear glass electrode can be preset when multiple glass electrodes need to be ground. The position of the glass electrode is accelerated, a tension knob 213 is rotatably installed on one side of the first rotating seat 211, a second rotating seat 214 is rotatably installed in the groove of the first rotating seat 211, the tension knob 213 acts on the second rotating seat 214, a connecting block 215 is installed on the top of the second rotating seat 214, a vertical operating arm 221 is installed on the top of the connecting block 215, a first moving screw 222 is rotatably installed on the top of the vertical operating arm 221, the vertical operating arm 221 is slidably connected with a joint block 223, and the first moving screw 222 It is threadedly connected to the common block 223, and a horizontal operating arm 224 is slidably connected to the common block 223. A second movable screw 225 is rotatably installed on one side of the horizontal operating arm 224. The second movable screw 225 is threadedly connected to the common block 223. A clamping block 226 for clamping the glass electrode is installed on one side of the horizontal operating arm 224 away from the second movable screw 225. The clamping block 226 is used to clamp the glass electrode. A fixing knob 227 is rotatably installed on the clamping block 226 to fix the position of the clamped glass electrode and reduce the probability of the glass electrode slipping during the grinding process.

[0036] In order to facilitate the observation of the grinding situation when grinding the glass electrode, Figure 1-Figure 3 As shown, a telescopic rod 301 is installed on the side of the top of the bottom plate 101 away from the fixed block 102, and a connecting plate 302 is installed on the top of the telescopic rod 301. The connecting plate 302 is slidably installed with a sliding block 303 through a through groove provided in the connecting plate 302, and a magnifying glass 304 is sleeved in the middle of the sliding block 303. The height of the magnifying glass 304 is controlled by the telescopic rod 301, and the position of the magnifying glass 304 is slid by the sliding block 303. The position of the magnifying glass 304 can be moved when the position of the glass electrode is adjusted.

[0037] When using the glass electrode grinding device, when the structure of the taken glass electrode is irregular and needs to be ground, the glass electrode is inserted into the clamp block 226, and then the fixing knob 227 is tightened to fix the glass electrode on the clamp block 226. After fixing, the first rotating seat 211 is rotated to a suitable angle, and then the second rotating seat 214 is rotated to drive the vertical operating arm 221 and the horizontal operating arm 224 at its top and the glass electrode to rotate. Through the rotation of the first rotating seat 211 and the second rotating seat 214, the position to be ground is rotated to a position parallel to the grinding plate 113, and then the first moving screw 222 and the second moving screw 225 are rotated to move the glass electrode downward to the top of the grinding plate 113. When grinding the glass electrode, the motor 112 is turned on to drive the grinding plate 113 to rotate, and the telescopic rod 301 is opened to drive the connecting plate 302 and the magnifying glass 304 to move to a suitable height, and then the position of the magnifying glass 304 is slid by the sliding block 303. The state of the glass electrode during grinding can be detected during grinding.

[0038] The above description is only a preferred specific implementation manner of the invention, but the protection scope of the invention is not limited thereto. Any technician familiar with the technical field can make equivalent substitutions or changes within the technical scope disclosed by the invention according to the technical scheme and inventive concept of the invention, which should be covered by the protection scope of the invention.

Claims

1. A precise grinding device for stereotaxic injection glass electrodes for animal experiments, comprising a bottom plate (101), a motor (112) and a grinding sheet (113) located on the top of the bottom plate (101), and a clamping block (226) located on the top of the bottom plate (101), characterized in that: A fixing block (102) is installed on one side of the top of the bottom plate (101), a dovetail groove (103) is provided on the top of the fixing block (102), a threaded hole (104) is provided on one side of the fixing block (102), a front and rear operating arm (201) with a trapezoidal side surface is slidably installed on the fixing block (102) through the dovetail groove (103), a fixing plate (202) is installed on one side of the front and rear operating arm (201), a connecting rod (203) is rotatably installed on the fixing plate (202), a moving knob (204) is fixed on one end of the connecting rod (203) close to the fixing plate (202), and a threaded head (205) is provided on one end of the connecting rod (203) away from the fixing plate (202); A support plate (111) is installed on the top of the bottom plate (101), a cavity is formed between the support plate (111) and the bottom plate (101), the motor (112) is located in the cavity formed by the support plate (111) and the bottom plate (101), and the motor (112) is installed on the bottom wall of the support plate (111).

2. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 1, characterized in that: A first rotating seat (211) having a groove shape in the middle is rotatably mounted on the top of one end of the front and rear operating arms (201) away from the fixed plate (202), and a scale line (212) for determining the rotation angle of the first rotating seat (211) is marked on the bottom circumference of the side wall of the first rotating seat (211).

3. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 2, characterized in that: A tension knob (213) is rotatably mounted on one side of the first rotating seat (211), a second rotating seat (214) is rotatably mounted in a groove of the first rotating seat (211), and the tension knob (213) acts on the second rotating seat (214).

4. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 3, characterized in that: A connecting block (215) is installed on the top of the second rotating seat (214), and a vertical operating arm (221) is installed on the top of the connecting block (215).

5. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 4, characterized in that: A first movable screw rod (222) is rotatably mounted on the top of the vertical operating arm (221), and a joint block (223) is slidably connected to the vertical operating arm (221), and the first movable screw rod (222) is threadedly connected to the joint block (223).

6. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 5, characterized in that: A horizontal operating arm (224) is slidably connected to the common connection block (223), and a second movable screw rod (225) is rotatably mounted on one side surface of the horizontal operating arm (224), and the second movable screw rod (225) is threadedly connected to the common connection block (223).

7. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 6, characterized in that: A clamping block (226) for clamping the glass electrode is installed on a side surface of the horizontal operating arm (224) away from the second moving screw rod (225), and a fixing knob (227) is rotatably installed on the clamping block (226).

8. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 1, characterized in that: A telescopic rod (301) is installed on the side of the top of the bottom plate (101) away from the fixed block (102), and a connecting plate (302) is installed on the top of the telescopic rod (301).

9. The precise grinding device for stereotactic injection glass electrodes for animal experiments according to claim 8, characterized in that: The connecting plate (302) is slidably mounted with a sliding block (303) through a through groove provided in the connecting plate (302), and a magnifying glass (304) is sleeved in the middle of the sliding block (303).

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