Chicken intestinal fluid enzymatic fermentation product, preparation method and application thereof, and organic fertilizer

Abstract

The present invention relates to a chicken intestinal fluid enzymatic hydrolysis and fermentation product, a preparation method and application thereof, and an organic fertilizer, and relates to the technical field of organic fertilizers. The preparation method comprises the following steps: subjecting the chicken intestinal fluid to high-energy electron beam irradiation treatment to obtain activated chicken intestinal fluid; adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte; adding a microbial agent to the chicken intestinal fluid enzymolyte for fermentation, filtering, collecting the supernatant, and concentrating and drying to obtain the chicken intestinal fluid enzymolysis and fermentation product. The present invention subjects the chicken intestinal fluid to enzymolysis and fermentation to convert it into a chicken intestinal fluid enzymolysis and fermentation product having stable properties, a rich mellow aroma, and a fatty aroma, and subsequently further uses it in combination with an appropriate amount of decomposed organic fertilizer and a Chinese medicine extract to obtain a special organic fertilizer that can significantly increase the yield of angelica sinensis. This innovative achievement not only has significant economic benefits, but also provides strong support for the high quality and high yield of Chinese medicinal materials.

Description

Technical Field

[0001] The invention relates to the technical field of organic fertilizers, and in particular to a chicken intestinal fluid enzymatic hydrolysis and fermentation product, a preparation method and application thereof, and an organic fertilizer. Background Art

[0002] In the field of organic fertilizer technology, the search for efficient, environmentally friendly fertilizers that improve crop yield and quality has always been a hot topic. While rich in a variety of nutrients, traditional organic fertilizers often suffer from slow release and limited specificity, making them incapable of meeting the nutrient needs of specific crops. This is particularly true for medicinal crops, such as angelica, which have specialized nutrient requirements during their growth, making traditional organic fertilizers unable to meet their high-yield and high-quality demands.

[0003] On the other hand, chicken intestinal fluid, as an organic waste, is rich in protein, fat, and various trace elements, and has potential fertilizer value. However, the direct application of raw chicken intestinal fluid has many inconveniences, such as easy corruption, odor, and uneven nutrient release, which limits its widespread application in agricultural production.

[0004] Therefore, how to effectively utilize chicken intestinal fluid, an organic waste, and convert it into an efficient, environmentally friendly organic fertilizer that can meet the nutrient needs of specific crops has become an urgent problem to be solved in the current field of organic fertilizer technology. Summary of the Invention

[0005] In order to solve the above problems, the present invention provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, a preparation method and application thereof, and an organic fertilizer.

[0006] In a first aspect, the present invention provides a method for preparing a chicken intestinal fluid enzymatic fermentation product, the preparation method comprising the following steps:

[0007] The chicken intestinal fluid is irradiated with high-energy electron beam to obtain activated chicken intestinal fluid;

[0008] adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte;

[0009] adding a microbial agent to the chicken intestinal fluid enzymolyzate for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product;

[0010] Wherein, the complex enzyme comprises alkaline protease, enteroprotease and lipase in a weight ratio of (1-5):(1-3):(1-2);

[0011] The microbial agent includes Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.0×10 8 ~3.0×10 8 cfu / mL.

[0012] Furthermore, the working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation amount of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

[0013] Furthermore, the working condition parameters of the enzymatic hydrolysis include: pH 8.0-8.5, temperature 50-60° C., time 3-5 hours, and the added weight of the complex enzyme is 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

[0014] Furthermore, the fermentation working condition parameters include: temperature of 30-40° C., time of 25-35 h, and the added weight of the microbial agent of 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

[0015] Furthermore, the fermentation broth is filtered using a filter membrane with a molecular weight cut-off of 1500 to 1800 Da.

[0016] In a second aspect, the present invention provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, which is prepared by the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to any one of the first aspects.

[0017] In a third aspect, the present invention provides a use of the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to any one of the second aspects in preparing organic fertilizer.

[0018] In a fourth aspect, the present invention provides an organic fertilizer, comprising the enzymatic hydrolysis and fermentation product of chicken intestinal fluid according to any one of the second aspects.

[0019] Furthermore, the organic fertilizer comprises the following raw materials in parts by weight:

[0020] 30-50 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 80-120 parts of decomposed organic fertilizer, and 3-8 parts of Chinese herbal medicine extract;

[0021] The Chinese medicinal extract is obtained by adding alligator mouth flower and two-color limonium herb in a weight ratio of (39-47): (7-15) into water, performing reflux extraction, and concentrating and drying the water extract.

[0022] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0023] The present invention provides a chicken intestinal fluid enzymatic fermentation product, its preparation method, application, and organic fertilizer. The invention converts chicken intestinal fluid into a stable product with a rich, mellow, and fatty aroma through enzymatic hydrolysis and fermentation. This product is then combined with an appropriate amount of decomposed organic fertilizer and traditional Chinese medicine extracts to produce a special organic fertilizer that significantly increases angelica yield. This innovative achievement not only has significant economic benefits but also provides strong support for the high-quality and high-yield production of traditional Chinese medicine. DETAILED DESCRIPTION

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention are clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts are within the scope of protection of the present invention.

[0025] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0026] In a first aspect, the present invention provides a method for preparing a chicken intestinal fluid enzymatic fermentation product, the preparation method comprising the following steps:

[0027] The chicken intestinal fluid is irradiated with high-energy electron beam to obtain activated chicken intestinal fluid;

[0028] adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte;

[0029] adding a microbial agent to the chicken intestinal fluid enzymolyzate for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product;

[0030] Wherein, the complex enzyme comprises alkaline protease, enteroprotease and lipase in a weight ratio of (1-5):(1-3):(1-2);

[0031] The microbial agent includes Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.0×10 8 ~3.0×10 8 cfu / mL.

[0032] The present invention provides an organic fertilizer that converts chicken intestinal fluid into a stable, rich, mellow, and fatty chicken intestinal fluid enzymatic fermentation product through enzymatic hydrolysis and fermentation. This product is then combined with an appropriate amount of decomposed organic fertilizer and traditional Chinese medicine extracts to produce a unique organic fertilizer that significantly increases angelica yield. This innovative achievement not only has significant economic benefits but also provides strong support for the high-quality and high-yield production of traditional Chinese medicine.

[0033] The chicken intestinal fluid of the present invention refers to fresh chicken intestines from slaughterhouses that are crushed into 3-5 cm segments, added into water for steaming, solid chicken intestines are separated and concentrated to a liquid with a solid content of 55-65 wt%.

[0034] In the present invention, alkaline protease, intestinal protease, lipase, Bacillus velezensis, Bacillus subtilis, yeast and Lactobacillus plantarum can directly use commercial products without special restrictions. For example, alkaline protease can directly use the commercial product of Hebei Lihua Biotechnology Co., Ltd., intestinal protease can directly use the commercial product of Shanghai Fusheng Industrial Co., Ltd., lipase can directly use the commercial product of Cangzhou Xiasheng Enzyme Biotechnology Co., Ltd., Bacillus velezensis and Bacillus subtilis can directly use the commercial products of Shandong Yihao Biotechnology Co., Ltd., yeast can directly use the commercial product of Angel Yeast Co., Ltd., and Lactobacillus plantarum can directly use the commercial product of Guangzhou Huihe Biotechnology Co., Ltd.

[0035] In some specific embodiments, the working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation dose of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

[0036] In some specific embodiments, the working condition parameters of the enzymatic hydrolysis include: pH 8.0-8.5, temperature 50-60°C, time 3-5h, and the added weight of the complex enzyme is 0.01-0.05g per 1g of the activated chicken intestinal fluid.

[0037] In some specific embodiments, the fermentation working condition parameters include: temperature of 30-40° C., time of 25-35 h, and the added weight of the microbial agent is 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

[0038] In some specific embodiments, the fermentation broth is filtered using a filter membrane with a molecular weight cut-off of 1500-1800 Da, preferably 1700 Da.

[0039] In a second aspect, based on the same inventive concept, the present invention provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, which is prepared by the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product according to any one of the first aspects.

[0040] The chicken intestinal fluid enzymatic hydrolysis and fermentation product provided by the present invention is achieved based on the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product described in any one of the first aspects, and therefore has at least the beneficial effects of the organic fertilizer described in any one of the first aspects, which will not be repeated here.

[0041] In a third aspect, based on the same inventive concept, the present invention provides an application of the chicken intestinal fluid enzymatic hydrolysis and fermentation product described in any one of the second aspects in the preparation of organic fertilizer.

[0042] In a fourth aspect, the present invention provides an organic fertilizer, comprising the enzymatic hydrolysis and fermentation product of chicken intestinal fluid according to any one of the second aspects.

[0043] In some specific embodiments, the organic fertilizer comprises the following raw materials in parts by weight:

[0044] 30-50 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 80-120 parts of decomposed organic fertilizer, and 3-8 parts of Chinese herbal medicine extract;

[0045] The Chinese medicinal extract is obtained by adding alligator mouth flower and two-color limonium herb in a weight ratio of (39-47): (7-15) into water, performing reflux extraction, and concentrating and drying the water extract.

[0046] In the present invention, decomposed organic fertilizer can be made of commercially available decomposed chicken manure, decomposed cow dung, etc., or directly made by existing preparation technology, such as evenly mixing livestock and poultry manure (such as chicken manure, pig manure, cow dung, sheep dung, etc.) into compost fermented by composite microorganisms (such as lactic acid bacteria, Bacillus, photosynthetic bacteria, yeast, actinomycetes, acetic acid bacteria, etc.), livestock and poultry manure accounts for about 80% of the total weight of the compost and the composite microorganisms are 0.5% of the total weight of the compost material, and then piled into a 2-meter-wide and 1-meter-high strip for compost fermentation, that is, to obtain decomposed organic fertilizer. Specifically, the decomposed organic fertilizer is decomposed cow dung, which is directly made of commercially available decomposed cow dung (water content 30%, organic matter 15%) such as Hebei Xiong'an Xinying Technology Co., Ltd.

[0047] The Clinacanthus nutans (Burm.f.) Lindau in the present invention is a herbaceous plant of the genus Clinacanthus in the family Acanthaceae, and its aliases include: thousand-mile chase, soft thorn grass, Handi grass, bamboo yellow, bamboo leaf green, green arrow, twisted sequence flower, etc.

[0048] The Limonium bicolor (Bunge) Kuntze in the present invention is a perennial herbaceous plant of the genus Limonium in the family Plumbaginaceae, and is also known as scorpion flower, flea flower, wild spinach, and artemisia selengensis.

[0049] The preparation method of the organic fertilizer of the present invention comprises the following steps: stirring and mixing the components.

[0050] In some specific embodiments, the organic fertilizer comprises the following raw materials in parts by weight:

[0051] 45 parts of the chicken intestinal fluid enzymatic fermentation product according to any one of the second aspects, 100 parts of decomposed organic fertilizer, and 7 parts of Chinese herbal medicine extract;

[0052] The Chinese medicinal extract is obtained by adding alligator mouth flower and limonium bicolor in a weight ratio of 43:12 into water, performing reflux extraction, and concentrating and drying the water extract.

[0053] It should be noted that the component raw materials involved in the organic fertilizer provided in the embodiment of the present invention and its preparation method and application, unless otherwise specified or specified, can directly use commercially available products or use existing public preparation methods to make them themselves; at the same time, the steps and parameters involved, unless otherwise specified or specified, can be carried out according to the preparation process and parameter conditions disclosed in the prior art or directly using existing equipment, and the present invention document will not repeat them one by one.

[0054] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended to illustrate the present invention only and are not intended to limit the scope of the invention. The experimental methods in the following examples where specific conditions are not specified are generally measured in accordance with national standards. If there are no corresponding national standards, then the methods are carried out in accordance with general international standards, conventional conditions, or the conditions recommended by the manufacturer.

[0055] Example 1

[0056] This example provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product comprises the following steps:

[0057] irradiating the chicken intestinal fluid with a high-energy electron beam, wherein the irradiation dose of the high-energy electron beam is 5 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0058] adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte;

[0059] adding a microbial agent to the chicken intestinal fluid enzymatic hydrolysate for fermentation, filtering the fermentation broth with a filter membrane with a molecular weight cutoff of 1700 Da, collecting the supernatant, and concentrating and drying to obtain the chicken intestinal fluid enzymatic fermentation product;

[0060] The complex enzyme comprises alkaline protease, intestinal protease and lipase in a weight ratio of 3:2:1.5; the working condition parameters of the enzymatic hydrolysis include: pH 8.3, temperature 55°C, time 4 hours, and the added weight of the complex enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0061] The microbial agent includes Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 2:1:1:1.5, and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the fermentation working condition parameters include: temperature of 35°C, time of 30h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0062] Example 2

[0063] This example provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product comprises the following steps:

[0064] irradiating the chicken intestinal fluid with a high-energy electron beam, wherein the irradiation dose of the high-energy electron beam is 3 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0065] adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte;

[0066] adding a microbial agent to the chicken intestinal fluid enzymatic hydrolysate for fermentation, filtering the fermentation broth with a filter membrane with a molecular weight cutoff of 1500 Da, collecting the supernatant, and concentrating and drying to obtain the chicken intestinal fluid enzymatic fermentation product;

[0067] The complex enzyme comprises alkaline protease, intestinal protease and lipase in a weight ratio of 1:1:1; the working condition parameters of the enzymatic hydrolysis include: pH 8.0, temperature 50°C, time 5 hours, and the added weight of the complex enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0068] The microbial agent includes Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 3:2:2:1, and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the fermentation working condition parameters include: temperature of 40°C, time of 25h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0069] Example 3

[0070] This example provides a chicken intestinal fluid enzymatic hydrolysis and fermentation product, and the preparation method of the chicken intestinal fluid enzymatic hydrolysis and fermentation product comprises the following steps:

[0071] irradiating the chicken intestinal fluid with a high-energy electron beam, wherein the irradiation dose of the high-energy electron beam is 8 KGy per 1 g of the chicken intestinal fluid, to obtain activated chicken intestinal fluid;

[0072] adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte;

[0073] adding a microbial agent to the chicken intestinal fluid enzymatic hydrolysate for fermentation, filtering the fermentation broth with a filter membrane with a molecular weight cutoff of 1800 Da, collecting the supernatant, and concentrating and drying to obtain the chicken intestinal fluid enzymatic fermentation product;

[0074] The complex enzyme comprises alkaline protease, intestinal protease and lipase in a weight ratio of 5:3:2; the working condition parameters of the enzymatic hydrolysis include: pH 8.5, temperature 60°C, time 3 hours, and the added weight of the complex enzyme is 0.03g per 1g of the activated chicken intestinal fluid;

[0075] The microbial agent includes Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of 1:1:1:1, and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum is 2.5×10 8 cfu / mL; the fermentation working condition parameters include: temperature of 30°C, time of 35h, and the added weight of the microbial agent is 0.02g per 1g of the activated chicken intestinal fluid.

[0076] Example 4

[0077] This example provides an organic fertilizer, which includes the following raw materials in parts by weight:

[0078] 45 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 1, 100 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 7 parts of Chinese herbal medicine extract;

[0079] The Chinese herbal extract is obtained by adding alligator beak flower and two-color blood-replenishing grass in a weight ratio of 43:12 to water for reflux extraction and concentrating and drying the water extract; wherein, the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator beak flower and the two-color blood-replenishing grass is 10:1.

[0080] Example 5

[0081] This example provides an organic fertilizer, which includes the following raw materials in parts by weight:

[0082] 30 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 2, 80 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 3 parts of Chinese herbal medicine extract;

[0083] The Chinese herbal medicine extract is obtained by adding alligator beak flower and two-color blood-replenishing grass in a weight ratio of 39:7 to water for reflux extraction and concentrating and drying the water extract; wherein, the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator beak flower and the two-color blood-replenishing grass is 10:1.

[0084] Example 6

[0085] This example provides an organic fertilizer, which includes the following raw materials in parts by weight:

[0086] 50 parts of the chicken intestinal fluid enzymatic fermentation product obtained in Example 3, 120 parts of decomposed organic fertilizer (specifically decomposed cow dung) and 8 parts of Chinese herbal medicine extract;

[0087] The Chinese herbal medicine extract is obtained by adding alligator flower and two-color blood-replenishing grass in a weight ratio of 47:15 to water for reflux extraction and concentrating and drying the water extract; wherein, the working condition parameters of the reflux extraction include: temperature of 105°C, time of 1 hour, number of times of 2 times, and the weight ratio of the total weight of the water, the alligator flower and the two-color blood-replenishing grass is 10:1.

[0088] Comparative Example 1

[0089] This example provides a chicken intestinal fluid enzymatic fermentation product and a preparation method thereof, which differs from Example 1 only in that the chicken intestinal fluid is not irradiated with a high-energy electron beam; the remaining steps and parameters are the same.

[0090] Comparative Example 2

[0091] This example provides an organic fertilizer, which differs from Example 4 only in that the enzymatic hydrolysis and fermentation product of chicken intestinal fluid is obtained by using Comparative Example 1; the remaining steps and parameters are the same.

[0092] Comparative Example 3

[0093] This example provides an organic fertilizer, which differs from Example 4 only in that the Chinese herbal extract is obtained by adding Limonium bicolor and Crocodile Mouth Flower in a weight ratio of 43:12 to water, performing reflux extraction, and concentrating and drying the water extract; the remaining steps and parameters are the same.

[0094] Test Case

[0095] This example investigates the promoting effect of the organic fertilizers obtained in Examples 4 to 6 and Comparative Examples 2 to 3 as a special base fertilizer for the cultivation of Angelica sinensis (the cultivated variety is Mingui No. 2), and a commercially available nitrogen-phosphorus-potassium compound fertilizer (15-15-15) is used as a basic control group.

[0096] 1. Experimental materials and instruments:

[0097] Organic fertilizer: the organic fertilizer obtained in Examples 4 to 6 and Comparative Example 2;

[0098] Angelica seeds: Mingui No. 2;

[0099] Ground film: used for covering cultivation;

[0100] High performance liquid chromatography: used to determine the ferulic acid content in angelica.

[0101] 2. Experimental method: each test group only uses the organic fertilizer obtained by embodiment 4-6 and comparative example 2-3 and the basal control group and uses commercially available nitrogen-phosphorus-potassium compound fertilizer respectively. 60kg / mu is applied as a disposable base fertilizer during soil preparation. Except that each test group uses different fertilizers, other growth management methods are all the same. No topdressing is applied. Film mulching is adopted for cultivation. The row spacing of angelica seedlings is 0.3m × 0.4m. Three groups of repetitions are set for each test group. The yield of angelica is counted after the angelica is harvested, and the ferulic acid content of angelica is measured by high performance liquid chromatography. The measurement results are averaged, and according to the formula: angelica fresh root yield increase rate (%)=(experimental group angelica fresh root yield-basic control group angelica fresh root yield) / basic control group angelica fresh root yield. Test result is as shown in Table 1.

[0102] Table 1

[0103] Test Group Increase rate of fresh root yield of Angelica sinensis (%) Example 4 group 31％ Example 5 group 22％ Example 6 group 24％ Comparative Example 2 13％ Comparative Example 3 10％

[0104] As can be seen from Table 1, the organic fertilizers provided in Examples 4 to 6 of the present invention can significantly increase the yield of angelica sinensis, can be used as special fertilizers for angelica sinensis cultivation, and have a better promoting effect on angelica sinensis cultivation than the organic fertilizers provided in Comparative Examples 2 to 3; In addition, the ferulic acid content of the angelica sinensis obtained in Example 4 was measured by high performance liquid chromatography, and the ferulic acid content (%) was as high as 0.150, indicating excellent quality.

[0105] In summary, the present invention provides a chicken intestinal fluid enzymatic fermentation product, its preparation method, application, and organic fertilizer. The invention converts chicken intestinal fluid into a stable, rich, mellow, and fatty chicken intestinal fluid enzymatic fermentation product through enzymatic hydrolysis and fermentation. This product is then combined with an appropriate amount of decomposed organic fertilizer and traditional Chinese medicine extracts to produce a special organic fertilizer that significantly increases angelica yield. This innovative achievement not only has significant economic benefits but also provides strong support for the high-quality and high-yield development of traditional Chinese medicine.

[0106] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in a range format is only for convenience and brevity and should not be construed as an inflexible limitation on the scope of the invention; therefore, the range description should be considered to have specifically disclosed all possible subranges and single numerical values ​​within the range. For example, the description of a range from 1 to 6 should be considered to have specifically disclosed subranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. In addition, whenever a numerical range is indicated herein, it is intended to include any cited numeral (fractional or integer) within the indicated range.

[0107] The foregoing description is intended only to provide specific embodiments of the present invention, which will enable those skilled in the art to understand and implement the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not intended to be limited to the embodiments shown herein, but is intended to be accorded the widest scope consistent with the principles and novel features claimed herein.

Claims

1. An organic fertilizer, characterized in that The organic fertilizer comprises the following raw materials in parts by weight: 30-50 parts of chicken intestinal fluid enzymatic fermentation product, 80-120 parts of decomposed organic fertilizer and 3-8 parts of Chinese herbal medicine extract; The Chinese herbal medicine extract is obtained by adding crocodile mouth flower and two-color limonium herb in a weight ratio of (39-47): (7-15) into water, performing reflux extraction, and concentrating and drying the water extract; The preparation method of the chicken intestinal fluid enzymatic fermentation product comprises the following steps: The chicken intestinal fluid is irradiated with high-energy electron beam to obtain activated chicken intestinal fluid; adding a complex enzyme to the activated chicken intestinal fluid for enzymolysis to obtain a chicken intestinal fluid enzymolyte; The method comprises adding a microbial agent to the chicken intestinal fluid enzymolyzate for fermentation, filtering, collecting the supernatant, concentrating and drying, and obtaining the chicken intestinal fluid enzymolysis fermentation product; wherein the complex enzyme comprises alkaline protease, intestinal protease and lipase in a weight ratio of (1-5):(1-3):(1-2); and the microbial agent comprises Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum in a volume ratio of (1-3):(1-2):(1-2):(1-2), and the abundance of Bacillus velez, Bacillus subtilis, yeast and Lactobacillus plantarum are all 2.0×10 8 ~3.0×10 8 cfu / mL.

2. organic fertilizer according to claim 1, is characterized in that, The working condition parameters of the high-energy electron beam irradiation treatment include: the irradiation amount of the high-energy electron beam irradiation treatment is 3 to 8 KGy per 1 g of the chicken intestinal fluid.

3. organic fertilizer according to claim 1, is characterized in that, The working condition parameters of the enzymatic hydrolysis include: pH 8.0-8.5, temperature 50-60° C., time 3-5 hours, and the added weight of the complex enzyme is 0.01-0.05 g per 1 g of the activated chicken intestinal fluid.

4. organic fertilizer according to claim 1, is characterized in that, The working condition parameters of the fermentation include: a temperature of 30 to 40° C., a time of 25 to 35 hours, and an added weight of 0.01 to 0.05 g of the microbial agent per 1 g of the activated chicken intestinal fluid.

5. organic fertilizer according to claim 1, is characterized in that, The fermentation broth is filtered using a filter membrane with a molecular weight cut-off of 1500 to 1800 Da.