Method for improving rhizosphere microecology of tomato by pseudomonas moraviensis combined with arbuscular mycorrhizal fungi and application thereof

Abstract

The application discloses a method for improving the rhizosphere micro-ecology of tomatoes by using Pseudomonas moraviensis in combination with arbuscular mycorrhizal fungi and application thereof, wherein the strain HY-S70 belongs to Pseudomonas moraviensis and has a preservation number of CGMCC No.32673. Before planting tomatoes, arbuscular mycorrhizal fungi inoculum (preferably Glomus mosseae) is inoculated into soil at an inoculation amount of 30 spores / g inoculum; and 3-5 days after planting the tomatoes, Pseudomonas moraviensis bacterial liquid with a bacterial concentration of 1x10 9 CFU / ml is inoculated. The method provided by the application can activate phosphorus in soil, promote the growth of tomato plants, improve the rhizosphere microbial community structure of the tomato plants and optimize the rhizosphere micro-ecological environment of the tomato plants, and has a wide application prospect.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of microbial technology, in particular to a method for improving the rhizosphere microecology of tomatoes by using Pseudomonas moraviensis in combination with arbuscular mycorrhizal fungi and application thereof. BACKGROUND

[0002] Tomato is the largest vegetable crop in the world in terms of planting area, and is deeply loved by consumers due to its high content of vitamin C and organic acids. Greenhouse is the main planting method for tomatoes. However, unreasonable fertilizer input and management measures (such as continuous cropping) not only lead to soil compaction and nutrient imbalance, but also reduce the quality indicators of tomatoes such as soluble sugar, and affect the function of the rhizosphere microbial community.

[0003] Microbial inoculants are a new type of environmentally friendly fertilizer containing a large number of beneficial live bacteria and various natural active substances. When applied to soil, functional bacteria can promote crop growth and control soil-borne diseases through mechanisms such as activating nutrients (nitrogen fixation, phosphorus dissolution, potassium dissolution, etc.), producing plant growth hormones, and secreting antibiotic substances. Compared with single inoculation of bacteria, dual inoculation of beneficial bacteria and arbuscular mycorrhizal fungi helps to overcome the problems of poor environmental adaptability and unstable effect of single bacteria. On the one hand, the diversity of the strains increases the resource spectrum available to the microbial community and enhances its competitive ability; on the other hand, dual inoculation helps to integrate the functional advantages of different strains and has a synergistic effect in promoting plant root growth and improving the soil microecological environment.

[0004] Crop yield is closely related to the soil rhizosphere microbial community. Studies have shown that rhizosphere core microorganisms play a key role in resisting the invasion of soil-borne pathogens and maintaining plant health. Rhizosphere microorganisms not only affect soil matter circulation and energy flow, but also promote the formation of soil aggregate structure, increase soil organic matter content, and improve soil nutrient cycling. The use of biological fertilizers in agricultural production is an important trend in future agricultural development. The use of microbial inoculants or biological fertilizers to regulate crop growth can maintain their yield and improve crop quality, which will help to promote sustainable agricultural development and achieve the "double carbon" goal. SUMMARY

[0005] The present application aims to develop a method for improving the rhizosphere microecology of tomatoes by using Pseudomonas moraviensis in combination with arbuscular mycorrhizal fungi, in order to address the problems of soil compaction, nutrient imbalance, and reduced microbial diversity in tomato-growing soils. The technical solution of the present application can improve the growth indicators of tomato plants such as plant height and stem diameter, promote the growth of tomato seedling roots, and improve the rhizosphere microbial diversity and community structure of tomatoes.

[0006] The object of the present application can be achieved by the following technical solution:

[0007] In a first aspect, the present application protects a Pseudomonas moraviensis strain named HY-S70, which has been deposited with the China General Microbiological Culture Collection Center on November 21, 2024, at the address of No. 1, Beichen West Road, Yard 3, Chaoyang District, Beijing, with the accession number of CGMCC No. 32673.

[0008] The HY-S70 strain is isolated and screened from the rhizosphere of healthy melon in Huai'an, Jiangsu Province on September 23, 2022.

[0009] In a second aspect, the present application provides a microbial agent, which also contains the Pseudomonas moraviensis described above or a fermentation product containing the Pseudomonas moraviensis described above.

[0010] In a specific embodiment, the fermentation product is prepared by inoculating the Pseudomonas moraviensis HY-S70 described above into LB liquid medium for fermentation culture, and the liquid culture conditions are as follows: temperature 28-30℃, rotation speed 170-180 rpm, fermentation time 24-36 h, to obtain Pseudomonas moraviensis HY-S70 fermentation broth, and the bacterial concentration of the Pseudomonas moraviensis HY-S70 fermentation broth is ≥1×10 9 CFU / mL.

[0011] In a third aspect, the present application also protects the use of the Pseudomonas moraviensis described above in combination with arbuscular mycorrhizal fungi to improve the rhizosphere microecology of tomatoes.

[0012] In a specific embodiment, the present application also protects the use of the Pseudomonas moraviensis described above in combination with arbuscular mycorrhizal fungi to promote the growth of tomatoes.

[0013] In a fourth aspect, the present application protects a method for improving the rhizosphere microecology of tomatoes by using the Pseudomonas moraviensis described above in combination with arbuscular mycorrhizal fungi, which comprises the following steps: first, mixing arbuscular mycorrhizal fungi with planting soil, and after 3-5 days of transplanting tomato seedlings (three-leaf-one-heart), adding 30 ml of Pseudomonas moraviensis HY-S70 bacterial solution with a content of 1×10 9 CFU / mL to the tomato rhizosphere, so that the bacterial concentration reaches 1×10 7 CFU / g of soil or more.

[0014] In a specific embodiment, the present application also protects a method for promoting the growth of tomatoes by using the Pseudomonas moraviensis described above in combination with arbuscular mycorrhizal fungi.

[0015] The species of the arbuscular mycorrhizal fungus is not particularly specified, and the source is not particularly limited, and a conventional commercially available product can be used.

[0016] In a specific embodiment, the arbuscular mycorrhizal fungus is Glomus mosseae, preferably Glomus mosseae BGC HEB02, which is purchased from the Glomus mosseae Germplasm Resource Bank of Beijing Academy of Agriculture and Forestry Sciences. Glomus mosseae is propagated in a sorghum pot before inoculation. The inoculant includes a mixture of soil and sand, and contains spores, infected root segments, or hyphae of the Glomus mosseae fungus.

[0017] In a more specific embodiment, Glomus mosseae is prepared into a Glomus mosseae inoculant, and 1 g of the inoculant contains 30-40 spores.

[0018] Beneficial effects

[0019] 1. The present application screens a strain of Pseudomonas moraviensis HY-S70 with the functions of dissolving phosphorus and producing IAA. Compared with other strains, the addition of Pseudomonas moraviensis HY-S70 bacterial solution to the substrate significantly improves the growth indicators such as the height, stem diameter, and aboveground biomass of tomato seedlings, and significantly improves the root structure such as root length, root surface area, and root tip number.

[0020] 2. Through potting experiments, it is found that compared with not applying microbial inoculants and applying only microbial inoculants, the double inoculation of Pseudomonas moraviensis HY-S70 and arbuscular mycorrhizal fungus inoculants synergistically increases the biomass of tomato plants, improves the relative abundance of beneficial microorganisms, and improves the soil rhizosphere microbial community structure.

[0021] 3. The present application provides a microbial inoculant application method which is easy to operate, environmentally friendly, and has significant effects. The method has a significant promoting effect on the growth of tomato crops.

[0022] Preservation Instructions

[0023] Strain name: Pseudomonas moraviensis;

[0024] Latin name: Pseudomonas moraviensis;

[0025] Strain number: HY-S70;

[0026] Preservation agency: China General Microbiological Culture Collection Center;

[0027] Abbreviation of preservation agency: CGMCC;

[0028] Preservation address: No. 3, Beichen West Road, Beijing;

[0029] Preservation date: November 21, 2024;

[0030] Collection No. CGMCC No. 32673. BRIEF DESCRIPTION OF DRAWINGS

[0031] Figure 1 Effects of different bacterial agents on the plant height of tomato.

[0032] Figure 2 Effects of different bacterial agents on the stem diameter of tomato.

[0033] Figure 3 Effects of different bacterial agents on the fresh weight of tomato.

[0034] Figure 4 Effects of different bacterial agents on the dry weight of tomato.

[0035] Figure 5 Effects of different bacterial agents on the richness index of the rhizosphere bacterial community of tomato.

[0036] Figure 6 Effects of different bacterial agents on the Shannon diversity index of the rhizosphere bacterial community of tomato.

[0037] Figure 7 Effects of different bacterial agents on the Simpson index of the rhizosphere bacterial community of tomato.

[0038] Figure 8 Effects of different bacterial agents on the relative abundance of bacterial phylum in the rhizosphere of tomato.

[0039] Figure 9 Effects of different bacterial agents on the relative abundance of bacterial genus in the rhizosphere of tomato.

[0040] Figure 10 Effects of different bacterial agents on the beta diversity of the rhizosphere bacterial community of tomato.

[0041] Figure 11 Prediction of the rhizosphere bacterial community of tomato under different bacterial agent treatments. DETAILED DESCRIPTION

[0042] The technical solutions of the present application will be further described in detail through specific embodiments.

[0043] Unless specifically indicated, the terms used in the present application are all commonly used terms in the field, and the technical means used in the examples, such as strain acquisition, test methods, etc., are all conventional means known to those skilled in the art, and the reagents and products used are all commercially available.

[0044] The medium formula used in the following examples is as follows:

[0045] Pseudomonas selective medium (CFC): Specifically: weigh 49.4 g of CFC medium, 10 g of glycerol, heat and dissolve in 1 L of distilled water, divide each bottle of 200 mL, 121 ℃ high pressure sterilization for 15 minutes, cool to about 50 ℃, then add a Pseudomonas CFC selective medium additive to each bottle, mix well. Medium formula: gelatin proteose peptone 16 g, acid hydrolyzed casein 10 g, potassium sulfate 10 g, magnesium chloride 1.4 g, agar 12 g, before sterilization, add 8 g of agar per liter of medium.

[0046] Inorganic phosphorus solubilizing medium: glucose 10 g / L, ammonium sulfate 0.5 g / L, yeast extract 0.5 g / L, sodium chloride 0.3 g / L, potassium chloride 0.3 g / L, magnesium sulfate 0.3 g / L, ferrous sulfate 0.03 g / L, manganese sulfate 0.03 g / L, tricalcium phosphate 5 g / L, pH adjusted to 7.0-7.5; solid medium: add 15 g of agar powder to the above ingredients. After dispensing, sterilize at 121 ℃ for 15 minutes and store for later use.

[0047] Organic phosphorus solubilizing medium: glucose 10 g / L, ammonium sulfate 0.5 g / L, yeast extract 0.5 g / L, sodium chloride 0.3 g / L, potassium chloride 0.3 g / L, magnesium sulfate 0.3 g / L, ferrous sulfate 0.03 g / L, manganese sulfate 0.03 g / L, lecithin 0.2 g / L, calcium carbonate 1 g / L, pH adjusted to 7.0-7.5.

[0048] LB medium: tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, pH adjusted to 7.0-7.2.

[0049] Example 1, soil sample collection and Pseudomonas isolation and screening

[0050] Strain acquisition: On September 23, 2022, healthy melon rhizosphere soil was collected from Huai'an City, Jiangsu Province, and isolated and screened. 10 g of melon rhizosphere soil was placed in a triangular flask containing glass beads and 90 mL of sterile water, shaken at 30 ℃ and 170 rpm for 30 min, and then allowed to stand for 10 min. The soil suspension was diluted to 10 -3 、10 -4 、10 -5 The three concentrations were 100 μl, which were inoculated on Pseudomonas selective medium (CFC) plates and incubated at 30 ℃ in an inverted incubator for 2-5 days. Different types of typical single colonies were picked out and purified on plates multiple times to obtain single strains, which were stored in glycerol tubes at -80 ℃ in a refrigerator.

[0051] Example 2, determination of candidate strain growth-promoting properties and rescreening

[0052] A total of 115 strains were screened by inoculating them on inorganic and organic phosphorus medium plates and incubating them at 30°C for 3-4 days. The strains that produced larger phosphorus-dissolving halos were quantitatively determined by the molybdenum-antimony anti-colorimetric method.

[0053] Molybdenum-antimony anti-colorimetric method: the test strains were inoculated in LB liquid medium for activation, 5 mL of the seed liquid was inoculated into 50 mL of inorganic and organic phosphorus medium, respectively, and incubated at 30°C and 180 rpm for 5 days. 10 mL of the fermentation broth was centrifuged at 10,000 rpm for 10 min, and the effective phosphorus content in the supernatant was determined by the molybdenum-antimony anti-colorimetric method. The pH was also determined.

[0054] IAA production determination: the activated strains were inoculated in LB liquid medium containing L-tryptophan (200 mg / L) and incubated at 30°C and 180 rpm for 3 days. The bacterial suspension was centrifuged at 10,000 rpm for 10 min, and the supernatant was added with an equal volume of Salkowski colorimetric solution. The mixture was kept in the dark for 30 min, and the OD530 value was determined. The content of IAA in the fermentation broth was calculated by comparing with the standard curve of analytical pure IAA.

[0055] Siderophore determination: the test strains were incubated in LB liquid medium for 24 h, 2 μL of the bacterial liquid was spotted on CAS solid medium (containing CAS blue detection solution, 30.24 g / L PIPES, and 0.9% agarose), and incubated at 30°C for 3 d. The presence of yellow or red halos around the colonies was observed to determine the ability of the strains to produce siderophores.

[0056] The results are shown in Table 1. Nine strains of phosphorus-dissolving bacteria were obtained by screening on inorganic phosphorus medium plates. The ratio of the diameter of the phosphorus-dissolving halo to the diameter of the colony on the plate ranged from 2.06 to 3.76, and the D / d value of all the strains was greater than 2, indicating that these strains had good inorganic phosphorus-dissolving ability. The amount of dissolved phosphorus in the fermentation broth of all the phosphorus-dissolving strains ranged from 12.45 to 276.61 mg / L, with the highest amount of dissolved phosphorus in strain HY-S70, followed by strains HY-L9 and HY-L8. The 12 strains of phosphorus-dissolving bacteria were incubated in organic phosphorus liquid medium for 5 days, and the amount of dissolved phosphorus in the fermentation broth of all the strains ranged from 1.83 to 7.3 mg / L, indicating that these strains had better inorganic phosphorus-dissolving ability than organic phosphorus-dissolving ability.

[0057] The quantitative determination of the ability of the strains to produce IAA showed that the amount of IAA produced by all the strains was greater than 5.00 mg / L, with the highest amount of IAA produced by strain HY-S70, which was 10.77 mg / L. In addition, 9 of the test strains had the ability to produce siderophores.

[0058] Table 1 Promoting characteristics of different strains

[0059]

[0060] Note: From "+++" to "+" indicates the siderophore production ability from strong to weak (according to As / Ar); "-" means that the index is not observed.

[0061] Example 3, Application of the candidate strains in tomato seedling test

[0062] Preparation of the bacterial agent: 4 strains with better growth-promoting characteristics were selected from the 12 strains screened in Example 2 above, which were HY-L8, HY-L9, HY-S49 and HY-S70. The four strains were inoculated into LB liquid medium and cultured at 30°C, 180 rpm for 24h to obtain bacterial suspension.

[0063] Preparation of the biological seedling substrate: the prepared bacterial suspension was mixed with ordinary seedling substrate at an inoculation amount of 5% (volume ratio) and placed for 1-2d to obtain the biological seedling substrate.

[0064] The seedling test was carried out in the plastic greenhouse of Huai'an Agricultural Academy of Science. The following treatments were set: HY-L8, HY-L9, HY-S49 and HY-S70. Each treatment had 3 replicates, each containing 25 seedlings. Tomato seeds (variety: Aifanbaowang F1) were soaked and germinated before seedling, and the seeds with uniform germination were selected for seedling. After 28d of seedling, the plant height, stem diameter, SPAD, aboveground fresh weight and dry weight, and root-related indicators of each treatment were measured.

[0065] The results (Tables 2-3) showed that the plant height, stem diameter, fresh weight and dry weight per plant of the seedlings grown in the biological seedling substrate containing HY-S70 strain were significantly higher than those of the seedlings grown in the biological seedling substrate containing HY-L8 and HY-S49 strains. The root length and other root indicators of the seedlings grown in the biological seedling substrate containing HY-S70 strain were significantly higher than those of the seedlings grown in the biological seedling substrate containing other strains.

[0066] Table 2 Effect of different strains on tomato seedling growth indicators

[0067]

[0068] Table 3 Effect of different strains on tomato seedling root growth

[0069]

[0070] Example 4, Identification of the candidate strains

[0071] According to the experimental results in Examples 1-3, strain HY-S70 was selected for identification and subsequent potting application test.

[0072] Identification of the strain: The total DNA of the strain HY-S70 was extracted using the bacterial total DNA extraction kit. The 16S rDNA amplification was performed using the universal primers 27F / 1492R, the forward primer 27F: 5'-AGAGTTTGATCCTGGCTCAG-3'; the reverse primer 1492R: 5'-GGTTACCTTGTTACGACTT-3'. The sequencing results were subjected to BLAST homology comparison in the National Center for Biotechnology Information (NCBI) database, and the representative sequences with higher homology were downloaded. The multiple sequence alignment was performed using the ClustalW command built in MEGA 11.0, and after removing the interval, the best nucleic acid substitution model was found to be Kimura 2+Gamma distribution through automatic analysis, and the maximum likelihood phylogenetic tree with Bootstrap of 500 was constructed based on the model, and the phylogenetic tree was made using FigTree 1.4.4. The results showed that the strain HY-S70 was in the same branch as Pseudomonas moraviensis, with the highest similarity, and finally the strain HY-S70 was determined to be Pseudomonas moraviensis.

[0073] Example 5, Application of Pseudomonas combined with arbuscular mycorrhizal fungi in tomato pot experiment

[0074] Preparation of Pseudomonas agent: The strain HY-S70 was inoculated into LB liquid medium, and cultured at 30°C, 180 rpm for 24h in a shaking incubator. After the culture was completed, the OD value was adjusted with sterile water to make the concentration of each bacterial suspension at 10 9 CFU / ml, ready for use.

[0075] Preparation of arbuscular mycorrhizal fungal agent: Funneliformis mosseae BGCHEB02 was selected and purchased from the Arbuscular Mycorrhizal Fungal Germplasm Repository of Beijing Academy of Agriculture and Forestry Sciences. Before inoculation, Funneliformis mosseae was pre-cultured in a sorghum pot, and the expansion medium was river sand and soil. After planting sorghum for 2 months, it was inoculated into the potting soil planted with tomatoes, and the inoculant included a mixture of soil and sand, containing spores, infected root segments or mycelium of Funneliformis mosseae fungi, 1g of the agent containing 30-40 spores.

[0076] Pot experiment implementation: 4 treatments were set, respectively CK (without fungicide), HY-S70, AMF, HY-S70+AMF. Each pot was filled with 7 kg of soil, and each treatment had 6 replicates. Before transplanting, the cluster root fungus inoculant was mixed with the planting soil, and the inoculation amount was added according to 1.5% of the dry soil weight. Commercial organic fertilizer was added to each treatment, and the addition amount was 2% of the dry soil weight. Select tomato seedlings with consistent growth (three leaves and one heart) for transplanting. After 3-5 days of transplanting, Pseudomonas moravica HY-S70 bacterial liquid was added to the tomato rhizosphere. After 30 days of transplanting, the growth indicators and rhizosphere microbial community structure of tomato plants in each treatment group were determined.

[0077] The results are shown in Figures 1 to 4 The plant height of tomato in the treatment of adding composite inoculant HY-S70+AMF was increased by 24.37% compared with the treatment of HY-S70, by 36.14% compared with the treatment of AMF, and by 65.60% compared with the blank group;

[0078] The stem diameter of tomato in the treatment of adding composite inoculant HY-S70+AMF was increased by 18.15% compared with the treatment of HY-S70, by 35.06% compared with the treatment of AMF, and by 23.77% compared with the blank group;

[0079] The aboveground fresh weight of tomato in the treatment of adding composite inoculant HY-S70+AMF was increased by 33.95% compared with the treatment of HY-S70, by 52.50% compared with the treatment of AMF, and by 69.20% compared with the blank group;

[0080] The aboveground fresh weight of tomato in the treatment of adding composite inoculant HY-S70+AMF was increased by 33.95% compared with the treatment of HY-S70, by 52.50% compared with the treatment of AMF, and by 69.20% compared with the blank group;

[0081] In summary, the plant height, stem diameter, aboveground fresh weight and aboveground dry weight of tomato in the treatment of adding composite inoculant HY-S70+AMF were significantly higher than those in other treatment groups.

[0082] Example 6, Pseudomonas combined with cluster root fungus in improving tomato rhizosphere microbial community

[0083] Tomato rhizosphere microbial community determination: 0.5 g of rhizosphere soil samples from the CK control group and the HY-S70+AMF treatment group were collected, and total DNA was extracted using Qubit 3.0 according to the instructions. The quality of the DNA was detected by NanoDrop2000 and 1% agarose gel electrophoresis. The concentration and purity were determined. The primer sequences were 341F and 806R for 16S rDNA amplification. The forward primer 341F was 5'-CCTACGGGNGGCWGCAG-3'; the reverse primer 806R was 5'-GGACTACHVGGGTATCTAAT-3'. The amplification system was 5x amplification buffer 4 μL, 0.8 μL of upstream and downstream primers (5 μmol / L) each, 2.5 mmol / L dNTPs 2 μL, TransStart FastPfu polymerase 0.4 μL, template DNA 10 ng, and 20 μL. The program was pre-denaturation at 95°C for 3 min, 95°C for 30 s, 55°C for 30 s, 72°C for 30 s, 72°C for 10 min, 27 cycles, and 4°C storage. PCR product purification was performed using AMPure XP Beads. After purification, Qubit 3.0 was used for quantification. IIIumina DNA Prep Kit (IIIumina, CA, USA) was used to construct the sequencing library. The library quality was detected using the ABIStepOnePlus Real-Time PCR System (Life Technologies, USA), and the Novaseq 6000 PE250 mode pooling was used for sequencing (NovaSeq6000 S2Reagent Kit v1.5, Illumina, USA). The Alpha diversity and Beta diversity indices were calculated using the Gideao biological cloud platform software.

[0084] The results are shown in Figures 5 to 11 Compared with the control CK treatment, the rhizosphere dominance index and Shannon diversity index of the tomato treated with the complex microbial agent HY-S70+AMF were significantly increased;

[0085] At the level of relative abundance of rhizosphere bacteria, the relative abundance of Proteobacteria, Firmicutes, Actinobacteria and Gemmatimonadetes treated with the complex microbial agent HY-S70+AMF was significantly increased compared with the CK treatment. At the level of relative abundance of genus, the relative abundance of Pseudomonas, Staphylococcus and Corynebacterium treated with the complex microbial agent HY-S70+AMF was significantly increased compared with the CK treatment.

[0086] The non-metric multi-dimensional scaling (NMDS) was used to analyze the distance of microbial community and characterize the beta diversity. At genus level, the stress value was 0.029, which was less than 0.05, and the difference of bacterial community composition between different treatments could be well revealed, and it was preliminarily judged that the inoculation of the complex bacterial community affected the composition of the bacterial community.

[0087] The functional prediction analysis result shows that the predicted functions of the rhizosphere microbial community of the HY-S70+AMF treatment and the CK treatment are significantly different, for example, the relative abundances of amino acid metabolism, coenzyme and vitamin metabolism, carbohydrate metabolism and polyketone metabolism of the soil rhizosphere bacterial community of the HY-S70+AMF treatment are significantly higher than those of the CK soil.

[0088] In summary, the application provides a method for jointly applying Pseudomonas moraviensis and arbuscular mycorrhizal fungi, which can synergistically promote the growth of tomatoes and improve the bacterial diversity and community composition of the rhizosphere soil of tomatoes.

[0089] The above description of disclosed embodiments enables those skilled in the art to carry out or use the present application. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the application. Therefore, the present application will not be limited to these embodiments shown herein, but will accord with the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. The application of Pseudomonas moravica HY-S70 combined with arbuscular mycorrhizal fungi in improving the rhizosphere microecology of tomatoes is characterized by: The arbuscular mycorrhizal fungus is Pseudomonas mosei BGC HEB02, which was purchased from the Arbuscular Mycorrhizal Fungi Germplasm Resource Bank of Beijing Academy of Agriculture and Forestry Sciences; the Pseudomonas moravianus HY-S70 is classified and named Pseudomonas moravianus ( Pseudomonas moraviensis ), was deposited in the General Microbiology Center of China Culture Collection Administration on November 21, 2024. The deposit address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the deposit number is: CGMCC No.32673.

2. A method for improving the rhizosphere microecology of tomatoes using Pseudomonas moravica HY-S70 in combination with arbuscular mycorrhizal fungi, comprising the following steps: first, mixing the arbuscular mycorrhizal fungi described in claim 1 with planting soil; 3 to 5 days after transplanting tomato seedlings, adding 30 ml of 1×10 9 CFU / mL of the bacterial solution of Pseudomonas moravica HY-S70 according to claim 1, so that its bacterial concentration reaches 1×10 7 CFU / g is above 3000kJ.

3. The use according to claim 1 or the method according to claim 2, characterized in that The fungus is propagated in a sorghum pot before inoculation, and the inoculant includes a soil-sand mixture containing spores, infected root segments or hyphae of the fungus.

4. The use according to claim 1 or the method according to claim 2, characterized in that The Pipelobothrium mosseae is prepared into a Pipelobothrium mosseae inoculum agent for use, wherein 1 g of the inoculum agent contains 30 to 40 spores.

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