Cultivation method of selenium-rich agrocybe cylindracea and selenium-rich agrocybe cylindracea product

Abstract

The invention provides a selenium-rich agrocybe cylindracea cultivation method and a selenium-rich agrocybe cylindracea product, and belongs to the technical field of edible mushroom cultivation. According to the cultivation method of the selenium-rich agrocybe cylindracea, the organic peptide chelated selenium and the compost are mixed, then agrocybe cylindracea inoculation and cultivation are conducted, conventional inorganic selenium is replaced with organic selenium, and the cultivation method has the advantages that safety is achieved, the yield is increased, and the quality is improved. According to the invention, the organic peptide chelated selenium is added into a cultivation raw material, and the selenium-rich agrocybe cylindracea is produced through the transformation and fruiting of the agrocybe cylindracea hyphae by absorbing selenium. In one embodiment of the invention, when the content of the organic peptide chelated selenium is more than 900 mu g / kg of the cultivation material, the selenium content of the produced fresh agrocybe cylindracea is more than 0.1 mg / kg, which reaches the standard of selenium-rich products.

Description

Technical Field

[0001] The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a cultivation method of a selenium-enriched Agrocybe aegerita and a selenium-enriched Agrocybe aegerita product. Background Art

[0002] Agrocybe cylindracea, commonly known as tea mushroom, poplar mushroom, etc., is scientifically named Agrocybe cylindracea. It is delicious, crisp and tender, with a strong aroma and rich nutrition. It is reported to contain 29.51% protein (dry product), 0.16% crude fat, 0.06% crude fiber and 46.22% carbohydrates, mainly carbohydrates and protein. There are 17 kinds of amino acids that make up the protein in Agrocybe cylindracea, and the essential amino acids for the human body account for 27.94% of the total free amino acids; the trace element analysis results of minerals show: Zn 79.3μg / g, Fe 79.2μg / g, Cu 18.9μg / g, Mn 25.2μg / g, Mg 3021μg / g and Ca 3607μg / g. Traditional Chinese medicine believes that tea tree mushroom is mild, sweet and warm in nature, and has the effects of diuresis, moistening the stomach, clearing away heat, calming the liver, improving eyesight, strengthening the spleen, preventing cancer, anti-aging, and lowering blood pressure. It is one of the delicious and rare edible fungi and has always enjoyed the reputation of "China's Magic Mushroom".

[0003] After years of development, the output of Agrocybe aegerita has been increasing, but the price is generally low. The cultivation method of selenium-enriched Agrocybe aegerita can improve the quality of Agrocybe aegerita and is expected to increase the sales price of Agrocybe aegerita. As an essential trace element for the human body, selenium has antioxidant, anti-aging, anti-cancer and other effects, and also has chemical anti-cancer potential. In recent years, as the role of selenium has been continuously explored, more and more people have begun to pay attention to the research of selenium-enriched products. Selenium-enriched edible fungi are a type of food rich in selenium with edible fungi as carriers. These edible fungi are enriched with selenium in the growth environment and accumulate selenium by absorbing inorganic selenium or organic selenium in the culture medium. Some common selenium-enriched edible fungi include selenium-enriched shiitake mushrooms, selenium-enriched Ganoderma lucidum, selenium-enriched Agaricus bisporus, etc. Edible fungi are mostly enriched with selenium by adding sodium selenite to the culture medium, but sodium selenite is inorganic selenium, and there are problems such as converting inorganic selenium into organic selenium and low selenium enrichment. Summary of the invention

[0004] The invention provides a cultivation method of a selenium-rich Agrocybe oleracea and a selenium-rich Agrocybe oleracea product. The selenium content of the produced fresh Agrocybe oleracea product is above 0.1 mg / kg, reaching the standard of selenium-rich products.

[0005] The invention provides a cultivation method of selenium-enriched Agrocybe tumefaciens, comprising adding organic peptide chelated selenium into a culture medium of Agrocybe tumefaciens, wherein the concentration of the organic peptide chelated selenium in the culture medium is not less than 300 μg / kg.

[0006] In a preferred embodiment of the present invention, the cultivation material is prepared from the following raw materials in parts by weight: 30-40 parts of sawdust, 30-40 parts of cottonseed hulls, 15-25 parts of wheat bran, 1-2 parts of gypsum and 1-3 parts of lime.

[0007] In a preferred embodiment of the present invention, the organic peptide chelated selenium is added when preparing the culture medium.

[0008] In a preferred embodiment of the present invention, the method for preparing the culture medium comprises mixing sawdust, cottonseed hulls, wheat bran, gypsum, lime and organic peptide chelated selenium, mixing the mixture evenly, bagging it and sterilizing it.

[0009] In a preferred embodiment of the present invention, the culture medium is sterilized at a temperature of 121° C. for 90 minutes.

[0010] In a preferred embodiment of the present invention, the culture medium to which organic peptide chelated selenium is added is used for aseptic inoculation of Agrocybe aegerita, and then mycelium culture is carried out.

[0011] In a preferred embodiment of the present invention, the temperature of the mycelium culture is 20° C. to 25° C., the relative humidity of the air is 60% to 70%, and the compost is turned once every 10 to 15 days.

[0012] In a preferred embodiment of the present invention, the concentration of organic peptide chelated selenium in the culture medium is not less than 900 μg / kg.

[0013] In a preferred embodiment of the present invention, the concentration of organic peptide chelated selenium in the culture medium is not less than 1200 μg / kg.

[0014] The present invention also provides a selenium-enriched Agrocybe aegerita product cultivated by the cultivation method.

[0015] Beneficial effects: The present invention provides a method for cultivating selenium-rich tea tree mushrooms, wherein organic peptide chelated selenium is mixed with culture medium for inoculation and cultivation of tea tree mushrooms, and conventional inorganic selenium is replaced with organic selenium, which has the advantages of safety, increased yield, and improved quality. The present invention adds the organic peptide chelated selenium to the cultivation raw materials, and the tea tree mushroom mycelium absorbs and transforms selenium into mushrooms, thereby producing selenium-rich tea tree mushrooms. In one embodiment of the present invention, when the concentration of organic peptide chelated selenium in the culture medium is above 900 μg / kg, the selenium content of the fresh tea tree mushrooms produced is above 0.1 mg / kg, reaching the standard of selenium-rich products. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is the product packaging picture of organic peptide chelated selenium;

[0017] Figure 2 This is the cultivation site picture of Formula 1;

[0018] Figure 3This is the cultivation site picture of formula 2;

[0019] Figure 4 This is the cultivation site picture of formula 3;

[0020] Figure 5 This is the cultivation site picture of formula 4;

[0021] Figure 6 This is the cultivation site photo of Recipe 5. DETAILED DESCRIPTION

[0022] The invention provides a cultivation method of selenium-enriched Agrocybe tumefaciens, comprising adding organic peptide chelated selenium into a culture medium of Agrocybe tumefaciens, wherein the concentration of the organic peptide chelated selenium in the culture medium is not less than 300 μg / kg.

[0023] The culture medium of the invention is prepared from the following raw materials in parts by weight: 30-40 parts of sawdust, 30-40 parts of cottonseed hulls, 15-25 parts of wheat bran, 1-2 parts of gypsum and 1-3 parts of lime.

[0024] In a preferred embodiment of the present invention, the organic peptide chelated selenium is added when preparing the culture medium. The source of the organic peptide chelated selenium is not particularly limited. Figure 1 The products shown were purchased from Jiangxi Tianhe Edible Fungi Development Co., Ltd.

[0025] The present invention does not specifically limit the preparation method of the culture medium. It can be prepared by using conventional methods in the art and adding organic peptide chelated selenium to the culture medium. For example, in one embodiment, when preparing the culture medium, sawdust, cottonseed hulls, wheat bran, gypsum, lime and organic peptide chelated selenium are mixed evenly and then bagged. In the present invention, 1 kg of the culture medium is loaded into each bag, and then the culture medium is sterilized, and the sterilization temperature is 121° C. and the time is 90 minutes. The concentration of organic peptide chelated selenium in the culture medium of the present invention is not less than 300 μg / kg, such as not less than 900 μg / kg in the embodiment.

[0026] In a preferred embodiment of the present invention, the culture medium with organic peptide chelated selenium is sterilely inoculated with Agrocybe aegerita, and then the mycelium is cultured. The culture medium with the inoculated bacteria is moved into a culture room at 20°C to 25°C for culture. The culture room is required to be ventilated, clean, dark, and the relative humidity of the air is maintained at 60% to 70%. The pile is turned once every 10d to 15d to ensure uniform fungus growth, and the bag is generally full after 30d to 40d. After that, conventional mushroom management and harvesting are carried out.

[0027] The present invention also provides a selenium-enriched Agrocybe aegerita product obtained by the cultivation method.

[0028] The selenium content of the fresh Agrocybe aegerita produced in the embodiment of the present invention is above 0.1 mg / kg, which can meet the quality requirements of selenium-rich products.

[0029] In order to further illustrate the present invention, a cultivation method of a selenium-enriched Agrocybe aegerita and a selenium-enriched Agrocybe aegerita product provided by the present invention are described in detail below in combination with embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0030] Example 1

[0031] 1. Test Materials

[0032] Varieties: Agrocybe aegerita “Tea 3”, which has been disclosed in the article (Wang Hongxiu, Zhang Lujun, Sun Peng, Hu Jia, An Ying, Chen Xutao, Wei Yunhui, etc., Journal of Microbiology, 2024, 44(2): 19-32);

[0033] Materials: Liquid selenium ( Figure 1 ), 15×30×0.05cm polypropylene high-pressure bags, sawdust, cottonseed hulls and wheat bran, etc.

[0034] 2. Test methods

[0035] Tea tree mushrooms were cultivated with sawdust and cottonseed shells as the main materials, and different concentrations of liquid selenium were added to the culture medium. The effects of different concentrations of liquid selenium on the growth rate of tea tree mushroom mycelium, fruiting body yield, selenium content in fruiting bodies and economic traits of fruiting bodies were determined, and the optimal concentration ratio of liquid selenium added during tea tree mushroom cultivation was determined, and the optimal selenium-enriched culture conditions for tea tree mushroom fruiting bodies were obtained.

[0036] 1. Preparation of culture medium:

[0037] The culture material formula (m / m) is: 38% sawdust, 40% cottonseed hull, 20% wheat bran, 1% gypsum and 1% lime.

[0038] There are 5 treatments, 30 bags in each treatment, and the calculated milliliters of each formula are added into the culture medium.

[0039] Formula 1: No liquid selenium was added to the culture medium, as a control;

[0040] Formula 2: Add 1.125mL of Tianhe liquid selenium to the culture medium, with a selenium content of 300μg / kg;

[0041] Formula 3: Add 2.25mL of Tianhe liquid selenium to the culture medium, with a selenium content of 600μg / kg;

[0042] Formula 4: Add 3.375 mL of Tianhe liquid selenium to the culture medium, with a selenium content of 900 μg / kg;

[0043] Recipe 5: Add 4.5 mL of Tianhe liquid selenium to the culture medium, with a selenium content of 1200 μg / kg.

[0044] 2. Sterilization, inoculation, cultivation, and harvesting

[0045] Place the bagged culture medium in the sterilizer according to the serial number, sterilize at 121℃ and for 90 minutes.

[0046] After sterilization, move the culture medium to the inoculation site, cool it to room temperature and inoculate it aseptically; inoculation requires sterile conditions, and the inoculation box should be disinfected with an aerosol disinfection box for half an hour before inoculation. When inoculating, untie the rope at the bag mouth, pick up a piece of bacteria with tweezers and place it on the surface of the medium, and tie the bag mouth with a rope.

[0047] Move the inoculated culture material into a 25℃ culture room for cultivation. The culture room should be ventilated, clean, dark, and the relative humidity should be maintained at 60% to 70%. Turn the pile every 10 days or so to ensure uniform growth of the fungi. Generally, the bag is full in about 40 days. Then carry out routine mushroom management and harvesting.

[0048] 3. Results

[0049] The daily growth length of Agrocybe aegerita mycelium was measured, the yield of fresh Agrocybe aegerita was counted, and the selenium content of fresh Agrocybe aegerita was tested. The results are shown in Table 1 and Figures 2 to 6 As shown, by using the formula of the present invention to cultivate Agrocybe aegerita, fresh Agrocybe aegerita with a selenium content of more than 0.1 mg / kg can be produced, reaching the standard of selenium-rich products.

[0050] Table 1 Characteristics of fresh Agrocybe aegerita fruiting bodies

[0051]

[0052] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for cultivating selenium-enriched Agrocybe aegerita, characterized in that: The method comprises adding organic peptide chelated selenium into the culture medium of Agrocybe aegerita, wherein the concentration of the organic peptide chelated selenium in the culture medium is not less than 300 μg / kg.

2. The cultivation method according to claim 1, characterized in that: The culture medium is prepared from the following raw materials in parts by weight: 30-40 parts of sawdust, 30-40 parts of cottonseed hulls, 15-25 parts of wheat bran, 1-2 parts of gypsum and 1-3 parts of lime.

3. The cultivation method according to claim 1, characterized in that: The method comprises adding the organic peptide chelated selenium when preparing the culture medium.

4. The cultivation method according to claim 2 or 3, characterized in that: The preparation method of the culture medium comprises the following steps: mixing sawdust, cottonseed hulls, wheat bran, gypsum, lime and organic peptide chelated selenium, and sterilizing the mixture after mixing it evenly and bagging it.

5. The cultivation method according to claim 4, characterized in that: The culture medium is sterilized at a temperature of 121° C. for 90 minutes.

6. The cultivation method according to claim 1, characterized in that: The tea tree mushrooms were aseptically inoculated with culture medium added with organic peptide chelated selenium, and then mycelium culture was performed.

7. The cultivation method according to claim 6, characterized in that: The mycelium culture temperature is 20° C. to 25° C., and the relative air humidity is 60% to 70%, and the compost is turned once every 10 to 15 days.

8. The cultivation method according to claim 6, characterized in that: The concentration of organic peptide chelated selenium in the culture medium is not less than 900 μg / kg.

9. The cultivation method according to claim 6, characterized in that: The concentration of organic peptide chelated selenium in the culture medium is not less than 1200 μg / kg.

10. A selenium-enriched Agrocybe aegerita product cultivated using the cultivation method according to any one of claims 1 to 9.

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