Method for artificially cultivating truffle and tricholoma matsutake

Abstract

The invention discloses a method for artificially cultivating truffle and tricholoma matsutake, and relates to the technical field of truffle and tricholoma matsutake cultivation, S1, good pine tree seeds are screened for cultivating sterile saplings, then the pine tree seeds are disinfected, and then the disinfected pine tree seeds are cultivated in a sterile tissue culture bottle to germinate into saplings; s2, respectively inoculating truffle and tricholoma matsutake strains to the roots of the pine tree seedlings obtained in the step S1 in a sterile environment, and then placing the pine tree seedlings in a culture device in the sterile environment to be cultured for more than one month to form mycorrhizal seedlings; s3, the formed mycorrhizal seedlings are transferred into a greenhouse to be cultivated, then environmental conditions in the greenhouse are manually controlled for cultivation, and sporocarps of truffles and tricholoma matsutake are obtained. According to the method, the two strains which can be symbiotic with the pine trees are adopted for culturing, and compared with a single strain, the method not only widens the applicability, but also remarkably improves the success rate of mycorrhizal seedlings.

Description

Technical Field

[0001] The invention belongs to the technical field of truffle and matsutake cultivation, and specifically is a method for artificially cultivating truffle and matsutake. Background Art

[0002] Truffles and matsutake are both wild mushrooms. At present, they are mainly cultivated through semi-artificial cultivation or mycorrhizal synthetic cultivation. In mycorrhizal synthetic cultivation, the first step is to collect seeds and separate (spore separation method, tissue separation method) to obtain pure strains and mycelium of truffles and matsutake, and then artificially inoculate and infect sterile seedlings. The infected truffle seedlings or matsutake seedlings are then transplanted for afforestation. Generally, the forest land can produce fruiting bodies after 4 years.

[0003] A truffle mycorrhizal seedling cultivation method with publication number CN119234619A provides a truffle mycorrhizal seedling cultivation method, and relates to the field of plant cultivation technology. The truffle mycorrhizal seedling cultivation method mainly includes the cultivation of host seedlings, the truffles are sterilized and mixed with anthocyanins and folic acid-configured nutrients to prepare a suspension, the suspension is mixed with indoleacetic acid and a growth agent configured with tea saponin to prepare a bacterial agent, and the bacterial agent is inoculated into the host seedlings and then hardened and cultivated. The shortcomings of the prior art are overcome, the formation time of mycorrhizae is effectively shortened, and the growth of mycorrhizal seedlings is promoted, the survival rate of subsequent commercial seedlings transplanting is guaranteed, the loss of cultivation is comprehensively reduced, and the economic benefits are improved.

[0004] However, although the above patent has achieved certain results, it still has some significant defects. The above cultivation method relies on the cultivation of a single strain of bacteria. This method is not only limited in practicality, but also has a relatively low success rate of mycorrhizal seedlings. In addition, the degree of automation of water replenishment and nutrient supply is insufficient, and often relies on manual operation, which is not only time-consuming and labor-intensive, but also difficult to ensure that the mycorrhizal seedlings obtain stable and appropriate water and nutrient support during the growth process. Summary of the invention

[0005] In order to solve the problems raised by the above background technology, the present invention proposes a method for artificially cultivating truffles and matsutake mushrooms.

[0006] The purpose of the present invention can be achieved by the following technical solution: A method for artificially cultivating truffles and matsutake mushrooms, comprising the following steps:

[0007] S1. Selecting good pine seeds for cultivating sterile seedlings, then sterilizing the pine seeds, and then culturing the sterilized pine seeds in sterile tissue culture bottles to germinate into seedlings;

[0008] S2, inoculating the truffle and matsutake strains into the roots of the pine seedlings obtained in step S1 under a sterile environment, and then culturing them in a sterile culture device for more than one month to form mycorrhizal seedlings;

[0009] S3. The formed mycorrhizal seedlings are transferred to a greenhouse for cultivation. Afterwards, the environmental conditions in the greenhouse are artificially controlled to cultivate and obtain the fruiting bodies of truffles and matsutake mushrooms.

[0010] As a further preferred embodiment of the present technical solution: the culture device in step S2 comprises a box body, and a water replenishment mechanism is installed on the box body;

[0011] The water replenishment mechanism comprises an automatic rotation reset unit arranged on the top of the box body for automatically adding water in a quantitative manner, and the automatic rotation reset unit is provided with a cover opening unit capable of automatically opening the cover when reaching a specified position;

[0012] A transfer barrel for temporarily storing water and nutrient solution is arranged directly below the automatic rotation reset unit. The transfer barrel is connected to a No. 2 connecting pipe via a No. 1 hose and an adapter. A roller is arranged on the No. 2 connecting pipe, and an atomizing spray group for atomizing water and nutrient solution is arranged on the roller.

[0013] As a further preferred embodiment of the present technical solution: the automatic rotation and resetting unit includes a No. 2 fixed rod fixedly connected to the top of the box body, the No. 2 fixed rod is slidably connected to a lifting seat, the No. 2 fixed rod is also fixedly connected to a block, a No. 2 spring is arranged between the block and the lifting seat, the lifting seat is rotatably connected to a No. 1 connecting pipe, the No. 1 connecting pipe is fixedly connected to a water replenishing bucket, and the water replenishing bucket is provided with a water outlet.

[0014] As a further preferred embodiment of the present technical solution: the automatic rotation reset unit further comprises a support frame fixedly connected to the top of the box body, and a rack is fixedly connected to the support frame;

[0015] A gear matched with the rack is fixedly connected to the outer wall of the No. 1 connecting pipe.

[0016] As a further preferred embodiment of the present technical solution: the cover opening unit includes a No. 1 fixing rod fixedly connected to the outer wall of the water replenishing bucket, a connecting frame is slidably connected to the No. 1 fixing rod, a sealing cover is fixedly connected to the connecting frame, and a No. 1 spring is arranged between the connecting frame and the water replenishing bucket.

[0017] As a further preferred embodiment of the present technical solution: the cover opening unit further comprises a support platform fixedly connected to the top of the box body, and a second triangle block with an inclined top surface is arranged above the support platform;

[0018] A No. 1 triangle block matched with a No. 2 triangle block is fixedly connected to one end of the connecting frame away from the sealing cover.

[0019] As a further preferred embodiment of the present technical solution: the water replenishing mechanism further includes a spraying unit;

[0020] The spraying unit comprises a No. 2 servo motor which is arranged on the side wall of the box body through a motor seat, and the output end of the No. 2 servo motor is connected to a No. 2 connecting pipe through a transmission wheel set.

[0021] As a further preferred embodiment of the technical solution: a stirring unit is provided inside the transfer barrel;

[0022] The stirring unit comprises a rotating shaft rotatably connected to the inside of the transfer barrel, and stirring blades are evenly arranged on the rotating shaft.

[0023] As a further preferred embodiment of the present technical solution: a water inlet is provided on the transfer barrel, and the water inlet is provided directly below the water outlet.

[0024] As a further preferred embodiment of the technical solution: a storage groove is provided inside the box body, and the storage groove is provided directly below the drum.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] 1. In the present invention, two bacterial species that can coexist with pine trees are used for cultivation. Compared with a single bacterial species, this method not only broadens the practicality, but also significantly improves the success rate of mycorrhizal seedlings.

[0027] 2. In the present invention, the synergistic effect of the automatic rotation resetting unit and the cover opening unit is used to realize the automatic regular and quantitative water replenishment of the mycorrhizal seedlings, which not only saves labor costs, but more importantly ensures that the mycorrhizal seedlings obtain a stable and appropriate supply of water during the growth process, thus saving labor costs for the method of cultivating truffles and matsutake mushrooms.

[0028] 3. In the present invention, the transfer barrel can allow the addition of nutrient solution and nutrient fertilizer, and the stirring unit in the transfer barrel can automatically stir the nutrient solution and water to make them evenly mixed, providing balanced nutritional support for the mycorrhizal seedlings, so that truffles and matsutake can grow rapidly under the best growth conditions in this cultivation method, shortening the cultivation cycle.

[0029] 4. In the present invention, the spraying unit in the water replenishment mechanism drives the No. 2 connecting pipe to rotate through the No. 2 servo motor, which in turn drives the atomizing spray group on the drum to rotate, so that the sprayed water mist is more uniform and can cover every corner in the storage tank, providing a more balanced growth environment for the mycorrhizal seedlings. The uniform atomizing spraying not only avoids the problem of growth deformity caused by local water shortage, but also helps to improve the yield and quality of truffles and matsutake. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 is a schematic diagram of the three-dimensional structure of the culture device;

[0031] Figure 2 for Figure 1 Schematic diagram of the local three-dimensional structure Figure 1 ;

[0032] Figure 3 for Figure 1 Schematic diagram of the local three-dimensional structure Figure 2 ;

[0033] Figure 4 for Figure 1 The enlarged schematic diagram at A in the middle;

[0034] Figure 5 for Figure 1 Schematic diagram of the local three-dimensional structure Figure 3 ;

[0035] Figure 6 for Figure 1 Partial structural section view.

[0036] Legend: 1. Box; 2. Storage tank; 3. Water replenishment mechanism; 31. Opening unit; 311. Fixed rod No. 1; 312. Spring No. 1; 313. Connecting frame; 314. Triangular block No. 1; 315. Sealing cover; 316. Support platform; 317. Triangular block No. 2; 32. Automatic rotation reset unit; 321. Fixed rod No. 2; 322. Stopper; 323. Spring No. 2; 324. Lifting seat; 325. Connecting pipe No. 1; 326. Gear 327, gear; 328, water supply bucket; 329, water outlet; 33, transfer bucket; 331, water inlet; 332, No. 1 hose; 333, adapter; 34, stirring unit; 341, No. 1 servo motor; 342, rotating shaft; 343, stirring blade; 35, spray unit; 351, No. 2 servo motor; 352, transmission wheel group; 353, No. 2 connecting pipe; 354, drum; 355, atomizing spray group; 4, No. 2 hose. DETAILED DESCRIPTION

[0037] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Embodiment 1:

[0039] See also Figure 1-Figure 6 The present application provides a method for artificially cultivating truffles and matsutake mushrooms, which is characterized by comprising the following steps:

[0040] S1. Select good pine seeds for cultivating sterile seedlings, then sterilize the pine seeds to ensure sterility, and then culture the sterilized pine seeds in sterile tissue culture bottles to germinate into seedlings;

[0041] S2, inoculating the truffle and matsutake strains into the roots of the pine seedlings obtained in step S1 under a sterile environment, so that the strains and the pine seedlings form a symbiotic relationship, and then placing them in a sterile culture device for culture for more than one month until the hyphae are successfully attached to the mycorrhiza of the pine seedlings to form mycorrhizal seedlings;

[0042] S3. The formed mycorrhizal seedlings are transferred to a greenhouse for cultivation. The environmental conditions in the greenhouse are artificially controlled, including appropriate temperature, humidity, carbon dioxide concentration and light, to promote the growth of truffles and matsutake mushrooms. After a period of cultivation, the fruiting bodies of truffles and matsutake mushrooms can be harvested.

[0043] Embodiment 2:

[0044] On the basis of the first embodiment, the culture device in step S2 includes a box body 1, on which a water replenishment mechanism 3 is installed, and the water replenishment mechanism 3 includes an automatic rotation reset unit 32 for automatically adding water in a quantitative manner arranged on the top of the box body 1, and the automatic rotation reset unit 32 is provided with a cover opening unit 31 that can automatically open the cover when reaching a specified position, and a transfer barrel 33 for temporarily storing water and nutrient solution is arranged directly below the automatic rotation reset unit 32, and the transfer barrel 33 is fixedly connected to the top wall of the box body 1, and the transfer barrel 33 is connected to a second connecting pipe 353 through a No. 1 hose 332 and an adapter 333. It should be noted that one The first hose 332 is a hose connected to the bottom of the transfer barrel 33, which is convenient for the nutrient solution and water in the transfer barrel 33 to flow out. A valve is provided between the transfer barrel 33 and the first hose 332. Specifically, a solenoid valve can be used to close the valve during the process of adding nutrient solution to ensure that the nutrient solution and water are fully dissolved. The adapter 333 and the second connecting pipe 353 are rotatably connected. A rotatable connector is used to ensure that the rotation of the second connecting pipe 353 is not affected and a through connection can be ensured. The second connecting pipe 353 is provided with a roller 354, and the roller 354 is provided with an atomizing spray group 355 that can atomize water and nutrient solution.

[0045] The automatic rotation reset unit 32 includes a No. 2 fixed rod 321 fixedly connected to the top of the box body 1, and the No. 2 fixed rod 321 is slidably connected to a lifting seat 324, and the No. 2 fixed rod 321 is also fixedly connected to a stopper 322, and a No. 2 spring 323 is arranged between the stopper 322 and the lifting seat 324, and when the lifting seat 324 moves downward and the water in the water replenishment bucket 328 becomes lighter, the lifting seat 324 can be lifted up to achieve the reset of the lifting seat 324, and a No. 1 connecting pipe 325 is rotatably connected to the lifting seat 324, and the No. 1 connecting pipe 325 is fixedly connected to the water replenishment bucket 328, and the No. 1 connecting pipe 325 and the water replenishment bucket 328 are connected in a through and sealed manner, and the end of the No. 1 connecting pipe 325 away from the water replenishment bucket 328 is connected in a through manner with a No. 2 hose 4 for connecting to an external water pump, and a valve can be arranged at the interface , which is used to control the size of the water flow, and then control the time when the water replenishment bucket 328 is full of water, and then adjust the watering cycle. The water replenishment bucket 328 is provided with a water outlet 329. When the water replenishment bucket 328 rotates one hundred and eighty degrees, the water can be poured out from the opening of the water outlet 329. The automatic rotation reset unit 32 also includes a support frame fixedly connected to the top of the box body 1, and the support frame is fixedly connected with a rack 326, which can cooperate with the gear 327 to make the water replenishment bucket 328 rotate one hundred and eighty degrees during the descent of the water replenishment bucket 328, and a baffle is provided at the bottom of the rack 326 to prevent the gear 327 from disengaging from the rack 326. The outer wall of the No. 1 connecting pipe 325 is fixedly connected with a gear 327 used in conjunction with the rack 326, and the tooth gap between the rack 326 and the gear 327 must be able to ensure that the gear 327 drives the No. 1 connecting pipe 325 to rotate one hundred and eighty degrees.

[0046] Specifically, clean water is injected from the No. 2 hose 4 into the No. 1 connecting pipe 325 into the interior of the water replenishment barrel 328. As the water inside the water replenishment barrel 328 increases, the overall weight of the water replenishment barrel 328 increases, and then the lifting seat 324 can move downward, and with the joint action of the rack 326 and the gear 327, the No. 1 connecting pipe 325 rotates, and the No. 1 connecting pipe 325 drives the water replenishment barrel 328 to rotate, and the water replenishment barrel 328 drives the water outlet 329 to rotate one hundred and eighty degrees, and aligns with the water inlet 331. In the process of moving downward, the block 322 is compressed, and when the water cup inside the water replenishment barrel 328 is poured out, the elastic force of the No. 2 spring 323 can lift and reset the water replenishment barrel 328. At this point, the water replenishment barrel 328 can be automatically rotated according to the water's own gravity, which is convenient for subsequent quantitative water filling work.

[0047] In this embodiment, the cover opening unit 31 includes a No. 1 fixing rod 311 fixedly connected to the outer wall of the water replenishment bucket 328, and a connecting frame 313 is slidably connected to the No. 1 fixing rod 311, which plays the role of connecting and supporting the No. 1 triangular block 314, and a sealing cover 315 is fixedly connected to the connecting frame 313. It should be noted that the sealing cover 315 is specifically arranged at the intersection of the water replenishment bucket 328 and the water outlet 329, and can slide and fit with the water outlet 329. In order to ensure its sealing, a sealing ring needs to be put on the sealing cover 315 to ensure the sealing effect, so as to avoid water seepage and water waste during the rotation of the water replenishment bucket 328, and the connecting frame 313 and the water replenishment bucket 3 A No. 1 spring 312 is arranged between the casing 1 and the casing 28, and the No. 1 spring 312 is sleeved on the outer side of the No. 1 fixing rod 311, and is used to push the connecting frame 313 to drive the sealing cover 315 to reset. The opening cover unit 31 also includes a support platform 316 fixedly connected to the top of the casing 1, and a No. 2 triangular block 317 with an inclined top surface is arranged above the support platform 316. A No. 1 triangular block 314 used in conjunction with the No. 2 triangular block 317 is fixedly connected to one end of the connecting frame 313 away from the sealing cover 315. It should be noted that the No. 1 triangular block 314 is also arranged with the inclined surface facing upward in the initial position, and the No. 2 triangular block 317 is arranged at the terminal position after the No. 1 triangular block 314 rotates 180 degrees.

[0048] Specifically, when the water replenishment bucket 328 drives the water outlet 329 to rotate and is about to reach the end position, the inclined surface of the second triangle block 317 lifts the first triangle block 314, and the first triangle block 314 pushes the connecting frame 313 to move in the direction of the sealing cover 315. The first spring 312 is compressed and pushes the sealing cover 315 from completely not contacting the water outlet 329 to form a gap, thereby pouring the water in the water replenishment bucket 328 into the interior of the transfer bucket 33. When the weight of the water after pouring out becomes smaller, that is, the water replenishment bucket 328 rotates in the opposite direction to reset, the first spring 312 can also be used under the action of elastic force. The connecting frame 313 is lifted up, and then the sealing cover 315 is again engaged between the water replenishment barrel 328 and the water outlet 329 to form a seal, and water is refilled through the No. 2 hose 4. The water in the transfer barrel 33 flows into the drum 354 along the No. 1 hose 332, the adapter 333 and the No. 2 connecting pipe 353, and is sprayed here by the atomizing spray group 355. The mycorrhizal seedlings in the box body 1 are automatically replenished with water regularly and quantitatively by the weight of the water. There is no need for manual watering by the staff, which saves manual care, and the watering cycle can be changed by changing the size of the external water flow.

[0049] In this embodiment, a water inlet 331 is provided on the transfer barrel 33, and the water inlet 331 is arranged directly below the water outlet 329. When the water outlet 329 is rotated one hundred and eighty degrees, water can be poured into the water inlet 331. The shape of the water inlet 331 is large in top opening and small in bottom opening to prevent the problem of spilling of the poured aqueous solution.

[0050] In this embodiment, a storage tank 2 is provided inside the box 1, and the storage tank 2 is arranged directly below the drum 354. A transparent door is provided on the box 1, which can be made of glass, so as to facilitate observation of the cultivation status of truffle and matsutake mushroom species inside the box 1.

[0051] Embodiment three:

[0052] On the basis of the second embodiment, the water replenishment mechanism 3 also includes a spraying unit 35, and the spraying unit 35 includes a No. 2 servo motor 351 arranged on the side wall of the box body 1 through a motor seat, and the output end of the No. 2 servo motor 351 is connected to the No. 2 connecting pipe 353 through a transmission wheel group 352, which is used to drive the No. 2 connecting pipe 353 to rotate, and the No. 2 connecting pipe 353 is connected with the atomizing spray group 355. The atomizing spray group 355 on the drum 354 is an atomizing nozzle with integrated water absorption and spraying, which draws water from the inside of the drum 354 and sprays it out after atomizing.

[0053] Specifically, when water flows into the atomizing spray group 355, the sealing cover 315 is started to drive the transmission wheel group 352 to rotate, the transmission wheel group 352 drives the No. 2 connecting pipe 353 to rotate, the No. 2 connecting pipe 353 drives the roller 354 to rotate, and then the atomizing spray group 355 is rotated. Through the rotation of the atomizing spray group 355, the sprayed water mist is made more uniform, which provides a guarantee for the formation of mycorrhizal seedlings and does not cause problems of local water shortage and growth deformity.

[0054] Embodiment 4:

[0055] On the basis of the third embodiment, a stirring unit 34 is provided inside the transfer barrel 33, and the stirring unit 34 includes a lifting seat 324 rotatably connected to the inside of the transfer barrel 33, and stirring blades 343 are evenly arranged on the rotating shaft 342 for stirring after adding the nutrient solution, and a servo motor 341 for driving the rotating shaft 342 to rotate is installed on the outer wall of the transfer barrel 33 to provide necessary drive.

[0056] Specifically, the nutrient solution can be added into the transfer barrel 33, and the first servo motor 341 is started to drive the rotating shaft 342 to rotate, and the rotating shaft 342 is driven to rotate to stir the nutrient solution and water inside, thereby making them evenly mixed.

[0057] The above embodiments are only used to illustrate the technical method of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical method of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical method of the present invention.

Claims

1. A method for artificially cultivating truffles and matsutake mushrooms, characterized in that: The following steps are involved: S1. Selecting good pine seeds for cultivating sterile seedlings, then sterilizing the pine seeds, and then culturing the sterilized pine seeds in sterile tissue culture bottles to germinate into seedlings; S2, inoculating the truffle and matsutake strains into the roots of the pine seedlings obtained in step S1 under a sterile environment, and then culturing them in a sterile culture device for more than one month to form mycorrhizal seedlings; S3. The formed mycorrhizal seedlings are transferred to a greenhouse for cultivation. Afterwards, the environmental conditions in the greenhouse are artificially controlled to cultivate and obtain the fruiting bodies of truffles and matsutake mushrooms.

2. The method for artificially cultivating truffles and matsutake mushrooms according to claim 1, characterized in that: The culture device in step S2 comprises a box (1), and a water replenishment mechanism (3) is installed on the box (1); The water replenishment mechanism (3) comprises an automatic rotation reset unit (32) arranged on the top of the box body (1) for automatically adding water in a quantitative manner, and the automatic rotation reset unit (32) is provided with a cover opening unit (31) capable of automatically opening the cover when reaching a specified position; A transfer barrel (33) for temporarily storing water and nutrient solution is arranged directly below the automatic rotation reset unit (32); the transfer barrel (33) is connected to a second connecting pipe (353) via a first hose (332) and an adapter (333); a roller (354) is arranged on the second connecting pipe (353); and an atomizing spray group (355) for atomizing water and nutrient solution is arranged on the roller (354).

3. The method for artificially cultivating truffles and matsutake mushrooms according to claim 2, characterized in that: The automatic rotation reset unit (32) comprises a No. 2 fixed rod (321) fixedly connected to the top of the box body (1); a lifting seat (324) is slidably connected to the No. 2 fixed rod (321); a stopper (322) is also fixedly connected to the No. 2 fixed rod (321); a No. 2 spring (323) is provided between the stopper (322) and the lifting seat (324); a No. 1 connecting pipe (325) is rotatably connected to the lifting seat (324); a water replenishment bucket (328) is fixedly connected to the No. 1 connecting pipe (325); and a water outlet (329) is provided on the water replenishment bucket (328).

4. The method for artificially cultivating truffles and matsutake mushrooms according to claim 3, characterized in that: The automatic rotation reset unit (32) further comprises a support frame fixedly connected to the top of the box body (1), and a rack (326) is fixedly connected to the support frame; A gear (327) matched with the rack (326) is fixedly connected to the outer wall of the No. 1 connecting pipe (325).

5. The method for artificially cultivating truffles and matsutake mushrooms according to claim 3, characterized in that: The cover opening unit (31) comprises a No. 1 fixing rod (311) fixedly connected to the outer wall of the water replenishing bucket (328), a connecting frame (313) is slidably connected to the No. 1 fixing rod (311), a sealing cover (315) is fixedly connected to the connecting frame (313), and a No. 1 spring (312) is provided between the connecting frame (313) and the water replenishing bucket (328).

6. The method for artificially cultivating truffles and matsutake mushrooms according to claim 5, characterized in that: The cover opening unit (31) further comprises a support platform (316) fixedly connected to the top of the box body (1), and a second triangle block (317) with an inclined top surface is arranged above the support platform (316); A No. 1 triangle block (314) matched with a No. 2 triangle block (317) is fixedly connected to one end of the connecting frame (313) away from the sealing cover (315).

7. The method for artificially cultivating truffles and matsutake mushrooms according to claim 2, characterized in that: The water replenishment mechanism (3) further comprises a spraying unit (35); The spraying unit (35) comprises a second servo motor (351) arranged on the side wall of the box body (1) via a motor seat, and the output end of the second servo motor (351) is connected to a second connecting pipe (353) via a transmission wheel set (352).

8. The method for artificially cultivating truffles and matsutake mushrooms according to claim 2, characterized in that: A stirring unit (34) is provided inside the transfer barrel (33); The stirring unit (34) comprises a rotating shaft (342) rotatably connected to the interior of the transfer barrel (33), and stirring blades (343) are evenly arranged on the rotating shaft (342).

9. The method for artificially cultivating truffles and matsutake mushrooms according to claim 3, characterized in that: The transfer barrel (33) is provided with a water inlet (331), and the water inlet (331) is arranged directly below the water outlet (329).

10. The method for artificially cultivating truffles and matsutake mushrooms according to claim 2, characterized in that: A storage groove (2) is provided inside the box body (1), and the storage groove (2) is arranged directly below the drum (354).

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