A mild exfoliating whitening body care composition and method of making and using same

Abstract

The present application relates to a kind of gentle exfoliating whitening body care composition and its preparation method and application, it is composed of the following weight parts of component: liposome 5-10 parts, papain 0.01-1 part and bromelain 0.005-0.5 part;Wherein, the liposome includes shell and core layer, the shell includes lecithin and phytosterol, the core layer includes camellia nitidissima extract and hydrolyzed royal jelly protein;The liposome includes the following weight parts of raw material: lecithin 2-15 parts, phytosterol 0.1-5 parts, camellia nitidissima extract 0.1-3 parts, hydrolyzed royal jelly protein 0.01-1 part.The composition can realize the multiple effects of skin lightening, removing old and useless cutin, tough skin, while safe, mild and non-irritating.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a mild exfoliating whitening and repairing body care composition and a preparation method and application thereof. BACKGROUND

[0002] Hand skin often faces problems such as pigmentation, roughness, aging, etc. due to exposure to external environment (such as ultraviolet rays, air pollution, etc.). Especially with age, the moisture loss and collagen reduction of hand skin, wrinkles and dullness are easily formed, affecting the overall appearance of the skin.

[0003] At present, there are various hand cream products on the market, aiming to improve the quality of hand skin, but most of the products have the problems of single function and slow effect, and there is also the limitation that they cannot effectively solve the multiple needs of skin lightening, waste keratin removal and skin strengthening. The existing technology often focuses on the care of one aspect, such as whitening or repair, but lacks comprehensive action, and many ingredients in the products may cause skin irritation or discomfort, affecting the use experience of consumers. Therefore, it is of wide market demand to develop a multi-effect hand cream that can lighten skin color, remove waste keratin and strengthen skin, and fill the gap of existing products in multiple functions. SUMMARY

[0004] The purpose of the present application is to overcome the deficiencies of the prior art and provide a mild exfoliating whitening and repairing body care composition and a preparation method and application thereof.

[0005] To achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:

[0006] In a first aspect, the present application provides a mild exfoliating whitening and repairing body care composition, which is composed of the following components by weight: liposomes 5-10 parts, papain 0.01-1 parts and bromelain 0.005-0.5 parts; wherein the liposomes include a shell layer and a core layer, the shell layer includes lecithin and phytosterol, and the core layer includes Lycianthes fraterna extract and hydrolyzed royal jelly protein; the liposomes include the following raw materials by weight: lecithin 2-15 parts, phytosterol 0.1-5 parts, Lycianthes fraterna extract 0.1-3 parts, and hydrolyzed royal jelly protein 0.01-1 parts.

[0007] The main components of the mild exfoliating whitening and repairing body care composition are liposomes, papain and bromelain, wherein the liposomes comprise a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises camellia japonica extract and hydrolyzed royal jelly protein. Specifically, the lecithin is a natural phospholipid containing abundant phosphatidylcholine, which can enhance the repair ability of skin cells and reduce skin dryness and laxity caused by aging; the phytosterol is a natural ingredient from plants with a similar structure to cholesterol, which has antioxidant and anti-inflammatory effects, can relieve oxidative stress and inflammatory aging of the skin, and at the same time, as a liposome shell, it can stabilize the liposomes and promote the release of the liposomes in the deep layer of the skin; the camellia japonica extract is rich in flavonoids and polysaccharide compounds, which have strong antioxidant and anti-inflammatory effects, can achieve the effects of whitening and skin protection by inhibiting tyrosinase activity and scavenging free radicals; the hydrolyzed royal jelly protein is a product with a smaller molecular weight obtained by hydrolyzing royal jelly protein with a protease, which is a high-nutrient substance that can be absorbed by the skin and can promote the repair and regeneration of the skin; the papain is a plant enzyme extracted from papaya, which has the effects of mild exfoliation and promoting cell renewal; the bromelain is a plant enzyme extracted from pineapple, which can deeply decompose the proteins in the stratum corneum, making the skin smoother, and the combination of the two can meet the exfoliation needs of different depths, and after the removal of old and useless keratin, it can promote the skin absorption and utilization of other active ingredients.

[0008] There are three reasons for wrapping the camellia japonica extract and the hydrolyzed royal jelly protein in the liposomes: 1. The camellia japonica extract is rich in flavonoids and polysaccharide compounds, which can affect the activity of papain and bromelain, so the camellia japonica extract needs to be separated from the two proteases; 2. The hydrolyzed royal jelly protein can also be decomposed by papain and bromelain, resulting in reduced activity, so the hydrolyzed royal jelly protein needs to be separated from the two proteases; 3. Papain and bromelain act on the surface of the skin, and after removing the old and useless keratin layer, they can promote the skin absorption of the camellia japonica extract and the hydrolyzed royal jelly protein, and wrapping the camellia japonica extract and the hydrolyzed royal jelly protein in liposomes can prevent their reaction with the proteases while further promoting their deep penetration into the deep layer of the skin.

[0009] Preferably, the weight ratio of the camellia japonica extract and the hydrolyzed royal jelly protein is (1.5-3.5):1.

[0010] More preferably, the weight ratio of the camellia japonica extract and the hydrolyzed royal jelly protein is (2-3):1.

[0011] Preferably, the mass ratio of the liposomes, papain and bromelain is 80:(1-5):(0.5-3).

[0012] More preferably, the mass ratio of the liposome, papain and bromelain is 80: (2-3): (1-2).

[0013] Specifically, the preparation method of the liposome comprises the following steps:

[0014] (1) adding lecithin and phytosterol into an ethanol solution, mixing uniformly, removing the ethanol to obtain a lipid film;

[0015] (2) mixing the extract of Camellia nitidissima and the hydrolyzed royal jelly protein uniformly, adding the lipid film, heating and stirring uniformly at 40-60℃, then homogenizing, obtaining the product, and then filtering out the uncoated components to obtain the liposome coated with the extract of Camellia nitidissima and the hydrolyzed royal jelly protein.

[0016] Preferably, in step (1) of the preparation method of the liposome, the mass concentration of the ethanol solution is 70-80%.

[0017] Preferably, in step (2) of the preparation method of the liposome, the pressure of homogenization is 500-800 bar, the rotation speed of homogenization is 1000-2000 rpm, and the time of homogenization is 3-5 min.

[0018] Specifically, the preparation method of the mild exfoliating whitening and repairing body care composition comprises the following steps:

[0019] After mixing the liposome, papain and bromelain, stirring at a rotation speed of 300-500 r / min for 10-20 min, the body care composition is obtained.

[0020] In the second aspect, the application provides the use of the mild exfoliating whitening and repairing body care composition in the first aspect in the preparation of skin care products.

[0021] In the third aspect, the application provides a hand cream in the second aspect, which comprises the following raw materials in percentage by weight: 5%-20% of the mild exfoliating whitening and repairing body care composition, 0.1%-0.3% of a thickening agent, 4%-6% of a humectant, 10-13% of oil phase raw materials, 1%-1.5% of a preservative and 0.1%-0.2% of a pH regulator, and the balance is deionized water.

[0022] Preferably, the thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyl dimethyl taurine sodium copolymer, ammonium acryloyl dimethyl taurine / VP copolymer, sordarins, and cetyl alcohol.

[0023] Preferably, the humectant includes at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, sodium hyaluronate, 1,2-butanediol, glycerin, pullulan, ceramide.

[0024] Preferably, the oil phase raw material includes at least one of dimethicone, cetearyl alcohol, caprylic / capric triglyceride, glyceryl stearate citrate and PEG-100 stearate.

[0025] Preferably, the preservative includes at least one of 1,2-propanediol, 1,2-hexanediol and p-hydroxyacetophenone.

[0026] Preferably, the pH regulator includes at least one of arginine, tromethamine and disodium EDTA.

[0027] In a fourth aspect, the present application provides a preparation method of the hand cream in the third aspect, comprising the following steps:

[0028] S1, the humectant, thickening agent and part of deionized water are mixed uniformly, and then are homogenized at 75-85 DEG C to form a uniform water phase; the oil phase raw material is mixed uniformly at 80-85 DEG C; the water phase and the oil phase raw material are mixed uniformly at 75-85 DEG C to obtain a mixture;

[0029] S2, the preservative is added to the mixture obtained in step S1, and is stirred uniformly; after the temperature is reduced to 35-45 DEG C, the body care composition and the remaining deionized water are added and stirred uniformly; finally, the pH regulator is added to adjust the pH to obtain the hand cream.

[0030] Compared with the prior art, the present application has the following beneficial effects:

[0031] The main components of the composition of the present application are papain, bromelain and liposomes of the shell layer of camellia sinensis extract and hydrolyzed royal jelly protein composed of lecithin and phytosterol. Papain and bromelain are both plant enzymes, and the combination of the two can meet the needs of different depths of exfoliation and effectively remove the old and worn-out stratum corneum. Camellia sinensis extract is rich in flavonoids and polysaccharide compounds, and has strong antioxidant and anti-inflammatory effects, thereby achieving the effect of whitening. Lecithin and phytosterol can play a repair effect, and at the same time, as a liposome shell, they can stabilize the liposomes and promote the release of the liposomes in the deep layer of the skin, helping the absorption and utilization of other active ingredients. Hydrolyzed royal jelly protein is a high-nutrient substance that can be absorbed by the skin, which can promote the repair and regeneration of the skin and accelerate the recovery of the skin to a healthy state. In addition, the camellia sinensis extract and the hydrolyzed royal jelly protein are wrapped in liposomes, which can prevent them from reacting with papain and bromelain and affecting the activity, and can promote them to penetrate into the deep layer of the skin to play a role, further enhancing the whitening and repair effects. Therefore, the synergistic effect of these components can achieve the multiple effects of mild exfoliation, whitening and repair. DETAILED DESCRIPTION

[0032] In order to better illustrate the purpose, technical scheme and advantages of the present application, the present application will be further described below in combination with specific examples.

[0033] The materials used in the following examples and comparative examples are as follows:

[0034] Lecithin: manufacturer is Shaanxi Danis Biological Technology Co., Ltd., model is DNS-2023021302;

[0035] Phytosterol: manufacturer is Shaanxi Danis Biological Technology Co., Ltd., model is DNS-2023081102;

[0036] Camellia sinensis extract: manufacturer is Ximu Source (Xiamen) Biological Technology Co., Ltd., model is Camellia sinensis extract;

[0037] Hydrolyzed royal jelly protein: manufacturer is Katakura & Co-op Agri Co., Ltd. of Japan, model is ROYALBIOCYTE;

[0038] Papain: manufacturer is Shaanxi Danis Biological Technology Co., Ltd., model is DNS-2023031503;

[0039] Bromelain: manufacturer is Shanxi Yirun Biological Technology Co., Ltd., model is 2024112108;

[0040] Other materials, reagents, etc. used in the application examples, application comparative examples, examples and comparative examples can be obtained from commercial channels if there is no special instruction.

[0041] Example 1

[0042] The mild exfoliating whitening body care composition is composed of the following components by weight: liposome 8 parts, papain 0.3 parts and bromelain 0.2 parts; wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises camellia sinensis extract and hydrolyzed royal jelly protein; the liposome comprises the following raw materials by weight: lecithin 6 parts, phytosterol 1 part, camellia sinensis extract 0.7 parts, and hydrolyzed royal jelly protein 0.3 parts.

[0043] The preparation method of the liposome comprises the following steps:

[0044] (1) lecithin and phytosterol are added to an ethanol solution with a mass concentration of 75%, mixed uniformly, and then the ethanol is removed to obtain a lipid film;

[0045] (2) the camellia sinensis extract and the hydrolyzed royal jelly protein are mixed uniformly, added to the lipid film, heated and stirred uniformly at 50°C, and then homogenized at a pressure of 600 bar and a rotation speed of 1500 rpm for 10 min, and the obtained product is filtered to remove the uncoated components to obtain the liposome coated with the camellia sinensis extract and the hydrolyzed royal jelly protein.

[0046] The preparation method of the mild exfoliating whitening body care composition comprises the following steps:

[0047] The liposome, papain and bromelain are mixed and stirred at a rotation speed of 400 r / min for 15 min to obtain the body care composition.

[0048] Example 2

[0049] The mild exfoliating whitening body care composition is composed of the following components by weight: liposome 8 parts, papain 0.3 parts and bromelain 0.2 parts; wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises camellia sinensis extract and hydrolyzed royal jelly protein; the liposome comprises the following raw materials by weight: lecithin 6 parts, phytosterol 1 part, camellia sinensis extract 0.7 parts, and hydrolyzed royal jelly protein 0.3 parts.

[0050] The preparation method of the liposome comprises the following steps:

[0051] (1) lecithin and phytosterol are added to an ethanol solution with a mass concentration of 75%, mixed uniformly, and then the ethanol is removed to obtain a lipid film;

[0052] (2) The Camellia sinensis extract and the hydrolyzed royal jelly protein are mixed uniformly, and then added into the lipid film. After being heated and stirred uniformly at 40℃, the obtained product is subjected to homogenization under the conditions of a pressure of 500 bar and a rotation speed of 1000 rpm for 30 min. Then, the un-wrapped components are filtered out, so as to obtain the liposome wrapping the Camellia sinensis extract and the hydrolyzed royal jelly protein.

[0053] The preparation method of the mild exfoliating whitening and repairing body care composition comprises the following steps:

[0054] The liposome, the papain and the bromelain are mixed, and then stirred at a rotation speed of 300 r / min for 20 min, so as to obtain the body care composition.

[0055] Example 3

[0056] The mild exfoliating whitening and repairing body care composition is composed of the following components in parts by weight: 10 parts of the liposome, 1 part of the papain and 0.5 parts of the bromelain; wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises the Camellia sinensis extract and the hydrolyzed royal jelly protein; the liposome comprises the following raw materials in parts by weight: 15 parts of lecithin, 5 parts of phytosterol, 3 parts of the Camellia sinensis extract and 1 part of the hydrolyzed royal jelly protein.

[0057] The preparation method of the liposome comprises the following steps:

[0058] (1) The lecithin and the phytosterol are added into an ethanol solution with a mass concentration of 80%, and then mixed uniformly. After the ethanol is removed, the lipid film is obtained.

[0059] (2) The Camellia sinensis extract and the hydrolyzed royal jelly protein are mixed uniformly, and then added into the lipid film. After being heated and stirred uniformly at 60℃, the obtained product is subjected to homogenization under the conditions of a pressure of 800 bar and a rotation speed of 2000 rpm for 5 min. Then, the un-wrapped components are filtered out, so as to obtain the liposome wrapping the Camellia sinensis extract and the hydrolyzed royal jelly protein.

[0060] The preparation method of the mild exfoliating whitening and repairing body care composition comprises the following steps:

[0061] The liposome, the papain and the bromelain are mixed, and then stirred at a rotation speed of 500 r / min for 10 min, so as to obtain the body care composition.

[0062] Example 4

[0063] The difference between Example 4 and Example 1 is that the weight ratio of the Camellia sinensis extract and the hydrolyzed royal jelly protein in the core layer is 1.5:1, while the addition amount of the raw materials is unchanged.

[0064] Example 5

[0065] Example 5 differs from Example 1 in that the weight ratio of the Camellia japonica extract and the hydrolyzed royal jelly protein in the core layer is 3.5:1, while the amount of the raw materials in the core layer remains unchanged.

[0066] Example 6

[0067] Example 6 differs from Example 1 in that the weight ratio of the Camellia japonica extract and the hydrolyzed royal jelly protein in the core layer is 2:1, while the amount of the raw materials in the core layer remains unchanged.

[0068] Example 7

[0069] Example 7 differs from Example 1 in that the weight ratio of the Camellia japonica extract and the hydrolyzed royal jelly protein in the core layer is 3:1, while the amount of the raw materials in the core layer remains unchanged.

[0070] Example 8

[0071] Example 8 differs from Example 1 in that the mass ratio of the liposome, the papain, and the bromelain is 80:1:3, while the total mass of the composition remains unchanged.

[0072] Example 9

[0073] Example 9 differs from Example 1 in that the mass ratio of the liposome, the papain, and the bromelain is 80:5:0.5, while the total mass of the composition remains unchanged.

[0074] Example 10

[0075] Example 10 differs from Example 1 in that the mass ratio of the liposome, the papain, and the bromelain is 80:2:2, while the total mass of the composition remains unchanged.

[0076] Example 11

[0077] Example 11 differs from Example 1 in that the mass ratio of the liposome, the papain, and the bromelain is 80:3:1, while the total mass of the composition remains unchanged.

[0078] Comparative Example 1

[0079] Comparative Example 1 differs from Example 1 in that the Camellia japonica extract is not added to the liposome of Comparative Example 1, and an equal amount of the hydrolyzed royal jelly protein is added to make up for the missing amount.

[0080] Comparative Example 2

[0081] Comparative Example 2 differs from Example 1 in that the hydrolyzed royal jelly protein is not added to the liposome of Comparative Example 2, and an equal amount of the Camellia japonica extract is added to make up for the missing amount.

[0082] Comparative Example 3

[0083] Comparative Example 3 differs from Example 1 in that no liposomes are added to Comparative Example 3, and an equivalent amount of lecithin, phytosterol, Lycium barbarum extract, and hydrolyzed royal jelly protein are added directly.

[0084] Comparative Example 4

[0085] Comparative Example 4 differs from Example 1 in that the total mass of the composition is unchanged, and no liposomes are added to Comparative Example 4, and the missing amount is made up with papain and bromelain in a mass ratio of 3:2.

[0086] Comparative Example 5

[0087] Comparative Example 5 differs from Example 1 in that the total mass of the composition is unchanged, and no papain is added to Comparative Example 5, and the missing amount is made up with liposomes and bromelain in a mass ratio of 80:2.

[0088] Comparative Example 6

[0089] Comparative Example 6 differs from Example 1 in that the total mass of the composition is unchanged, and no bromelain is added to Comparative Example 6, and the missing amount is made up with liposomes and papain in a mass ratio of 80:3.

[0090] Application Examples 1-11 and Comparative Application Examples 1-6

[0091] The compositions of Examples 1-11 and Comparative Examples 1-6 are added to hand creams at a concentration of 10 wt% to obtain hand creams of Application Examples 1-11 and Comparative Application Examples 1-6, and the formulations are shown in Table 1.

[0092] The preparation method of the hand creams of Application Examples 1-11 and Comparative Application Examples 1-6 comprises the following steps:

[0093] The preparation method of the hand creams comprises the following steps:

[0094] S1, the humectant, thickening agent, and 1 / 2 amount of deionized water are mixed, and a homogeneous aqueous phase is obtained by homogenization at 80°C; the oil phase raw materials are mixed uniformly at 85°C, and the aqueous phase and oil phase raw materials are mixed uniformly at 80°C to obtain a mixture;

[0095] S2, the preservative is added to the mixture obtained in step S1, and stirred uniformly; after the temperature drops to 40°C, the body care composition and the remaining deionized water are added and stirred uniformly; finally, the pH is adjusted to 6 by adding a pH adjuster, to obtain the hand cream.

[0096] Table 1 Formulations of the hand creams of Application Examples 1-11 and Comparative Application Examples 1-6

[0097]

[0098] Application Examples 12-13

[0099] Application Examples 12-13 differ from Application Example 1 in that the body care composition of Example 1 is added in an amount of 5 wt% in Application Example 12 and in an amount of 20 wt% in Application Example 13, and the remainder is completed with deionized water.

[0100] Application Comparative Example 7

[0101] The hand cream of Application Comparative Example 7 is not added with the composition, and an equal amount of deionized water is used instead of the composition, and the preparation method is the same as that of Application Example 1.

[0102] Performance Test

[0103] 1. Cell experiment proves that the composition exfoliates and increases collagen

[0104] Experimental method: The cell model used is a human 3D epidermal skin model (Guangdong Boxi Biological Technology Co., Ltd.), and the test conditions are: incubator temperature 37±1℃, saturated humidity, carbon dioxide 5±1%; according to the grouping, the 3D epidermal model is cultured and treated, and then tested, and the specific tests are the determination of the thickness of the stratum corneum and the content of type I collagen, and the test methods are as follows:

[0105] (1) The 3D epidermal skin model is cultured using EpiGrowth culture solution (Guangdong Boxi Biological Technology Co., Ltd.);

[0106] (2) 25 μL of 0.1% sodium dodecyl sulfate (Sigma) is applied to the surface of the 3D epidermal skin model and incubated for 30 minutes;

[0107] (3) The blank control group is evenly applied with 12.5 μL of the hand cream of Application Comparative Example 7 on the surface of the epidermal model, and the sample group is applied with 12.5 μL of the hand cream of Application Examples 1-13 and Application Comparative Examples 1-6, respectively, and cultured for 24 hours;

[0108] (4) After the culture is completed, the surface of the model is washed with PBS solution (Biosharp) to remove the remaining test substances, and then fixed with 4% paraformaldehyde (Biosharp) for 24 hours, and then cut and taken out;

[0109] (5) Stratum corneum thickness determination: the tissue sections are subjected to H&E staining (Bi Yun Tian), observed under a microscope, and the pictures are collected and analyzed;

[0110] (6) Collagen type I content detection: the tissue was lysed with RIPA cell lysis buffer containing protease inhibitors, the lysed sample was centrifuged at 12000 g for 5 minutes, the supernatant was taken, and the collagen type I content of the supernatant of each group of tissues was detected using a human collagen type I (Col I) ELISA kit (item number ml057630, Enzyme-linked Bio).

[0111] The rate of change of stratum corneum thickness and collagen of each example and comparative example was calculated using the following formula:

[0112] Stratum corneum thickness reduction rate (%) = (blank control group - sample group) / blank control group * 100%;

[0113] Collagen increase rate (%) = (sample group - blank control group) / blank control group * 100%; The results are shown in Table 2.

[0114] Table 2 Data of stratum corneum reduction rate and collagen increase rate of each group of hand cream samples

[0115] Group Decreasing rate of stratum corneum thickness / % Increasing rate of collagen / % Example 1 36.7 244 Example 2 35.1 204 Example 3 22.5 201 Example 4 28.6 225 Example 5 28.1 221 Example 6 33.4 236 Example 7 33.0 239 Example 8 29.2 223 Example 9 38.6 219 Example 10 32.7 232 Example 11 36.1 238 Example 12 34.4 232 Example 13 36.3 240 Comparative Example 1 27.8 108 Comparative Example 2 26.2 113 Comparative Example 3 28.9 63 Comparative Example 4 42.3 -10.3 Comparative Example 5 10.8 87 Comparative Example 6 11.5 93

[0116] Wherein, the higher the stratum corneum thickness reduction rate, the stronger the exfoliation effect of the hand cream; the higher the collagen increase rate, the stronger the skin strengthening effect of the hand cream.

[0117] 2. Human efficacy test of hand cream for exfoliation, whitening, repair and moisturizing

[0118] Experimental method: according to the "Cosmetic Safety Technical Specification" (2015) test, 120 Asian adult testers aged 18-60 with rough, dull and sensitive hand skin were selected and randomly divided into 20 groups, 6 people in each group. Volunteers applied the sample to both hands once a day in the morning and evening (sample: hand cream prepared according to application examples 1-13 and comparative examples 1-7), and visited and collected data on day 0 and day 28. After the volunteers visited, they washed their hands with hand sanitizer, sat in an air-conditioned room with a temperature of 21±1℃ and a humidity of 50±10% for 30 minutes, and then performed the following tests: the brightness L value of the back of the hand was measured using CK skin glossiness test probe GL200; the melanin content MI value of the back of the hand was measured using melanin skin detector Mexameter; the trans-epidermal water loss value TEWL of the back of the hand was measured using skin moisture loss test probe TewaMeter; and the skin moisture content of the back of the hand was measured using skin moisture test probe Corneometer.

[0119] The exfoliation ability is represented by the improvement of the skin brightness L value, the whitening ability is represented by the improvement of the melanin content MI value, the skin barrier repair ability is represented by the improvement of the transepidermal water loss TEWL value, and the moisturizing effect is represented by the improvement of the skin moisture content.

[0120] The formulae of the L value improvement rate, the MI value improvement rate, the TEWL value improvement rate and the skin moisture content improvement rate are as follows:

[0121] The L value improvement rate = (T28-T0) / T0*100%;

[0122] The MI value improvement rate = (T0-T28) / T0*100%;

[0123] The TEWL value improvement rate = (T0-T28) / T0*100%;

[0124] The skin moisture content improvement rate = (T28-T0) / T0*100%;

[0125] The results obtained are shown in Table 3.

[0126] Table 3 Exfoliation, whitening, repair and moisturizing human efficacy test results of the hand cream samples in each group

[0127] Group Improving rate of L value / % Improving rate of MI value / % Improving rate of TEWL value / % Improving rate of skin moisture content / % Example 1 15.4 18.9 23.3 37.6 Example 2 14.5 11.7 16.2 22.4 Example 3 16.2 19.1 17.4 29.6 Example 4 13.0 13.7 16.8 27.4 Example 5 13.5 13.5 15.2 26.0 Example 6 13.9 16.4 19.3 32.7 Example 7 14.1 16.9 19.8 33.8 Example 8 11.1 13.0 18.0 26.4 Example 9 15.3 18.6 15.1 29.5 Example 10 14.9 16.7 22.4 36.9 Example 11 14.8 18.5 20.1 34.0 Example 12 14.2 16.7 20.5 32.1 Example 13 15.1 18.4 22.9 36.3 Comparative Example 1 13.0 7.2 8.5 16.3 Comparative Example 2 13.4 7.8 8.4 17.0 Comparative Example 3 13.2 5.5 7.8 16.6 Comparative Example 4 16.2 9.3 5.9 7.1 Comparative Example 5 3.2 8.2 4.7 18.0 Comparative Example 6 5.9 8.5 6.0 19.9 Comparative Example 7 0.2 0.0 1.0 4.2

[0128] From Tables 2-3, in combination with the data of Application Examples 1 and 4-7, it can be known that the weight ratio of the honeysuckle extract and the hydrolyzed royal jelly protein in the liposome has a greater influence on the whitening, repair and moisturizing effects of the hand cream, so when the honeysuckle extract and the hydrolyzed royal jelly protein with a weight ratio of (2-3):1 are selected, the whitening, repair and moisturizing effects of the hand cream can reach a relatively optimal level.

[0129] In combination with the data of Application Examples 1 and 8-11, it can be known that the mass ratio of the liposome, the papain and the bromelain will affect the exfoliation, whitening, repair and moisturizing. When the content of the protease is high and the content of the liposome is low, the exfoliation and whitening performance is good, but the repair and moisturizing effects are obviously reduced. When the mass ratio of the liposome, the papain and the bromelain is 80:(2-3):(1-2), the exfoliation and repair of the hand cream are both at a relatively optimal level.

[0130] In combination with the data of Application Examples 1 and Comparative Examples 1-2, it can be known that the addition of the honeysuckle extract and the hydrolyzed royal jelly protein in the liposome can synergistically improve the whitening, moisturizing and repair performance of the hand cream.

[0131] From the data of application example 1 and application comparative example 3, it can be seen that the camellia sinensis extract and the hydrolyzed royal jelly protein are wrapped in the liposome, which can avoid decomposition of the two by protease, prevent reaction of them with protease, and further promote the two to penetrate into the deep layer of the skin to play a role.

[0132] From the data of application example 1 and application comparative examples 4-6, it can be seen that no liposome is added in application comparative example 4, and compared with application example 1, the stratum corneum thickness is obviously reduced, but the collagen content, repair and moisturizing effect are obviously reduced, because the lack of liposome buffers and repairs protease, and the damage to the stratum corneum is greater. In application comparative examples 5-6, no papain and bromelain is added, respectively, and the exfoliation, whitening, repair and moisturizing effects are lower than those of application example 1, which may be because the liposome, papain and bromelain synergistically act, and can realize the effects of mild exfoliation, whitening, repair and moisturizing.

[0133] 3. Human skin patch test.

[0134] Thirty volunteers, 27 males and 28 females, aged 20-50 years old, were recruited, and a closed patch test method was used. Equal amounts (0.020 mL-0.025 mL) of test samples (hand creams of application examples 1-13) were placed in a specific patch tester, which was attached to the arm of the volunteer with a low-sensitization adhesive tape, and was lightly pressed to evenly attach to the skin. The patch tester was left on the skin for 24 hours. The blank control group was distilled water, and the blank application example was a lotion without whitening and skin care hand cream. After 24 hours, the patch tester was removed, and the skin reaction was observed after 0.5 hours, 24 hours and 48 hours. The results were recorded. The adverse reaction grade of the skin is shown in Table 4 below.

[0135] Table 4 Adverse reaction grade table of the skin

[0136] Score level Skin reaction 0 Negative reaction 1 Suspected reaction, only weak erythema 2 Weak positive reaction (erythema reaction): erythema, infiltration, edema, and possibly papule 3 Strong positive reaction (vesicle reaction): erythema, infiltration, edema, and papule; reaction beyond the test area 4 Very strong positive reaction (fusion vesicle reaction): obvious erythema, severe infiltration, edema, and fusion vesicle; reaction beyond the test area

[0137] After testing, the hand creams of application examples 1-13 were all negative reactions after human patch test, and were safe and non-irritating to human skin.

[0138] In summary, the body care composition of the application applied to the hand cream can realize the multiple effects of brightening skin color, removing old and waste keratin, and strengthening skin, and is safe, mild and non-irritating.

[0139] Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the application and not to limit the protection scope of the application. Although the application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the application can be modified or replaced equivalently without departing from the essence and scope of the technical solutions of the application.

Claims

1. A mild exfoliating whitening repair body care composition characterized in that, The liposome is prepared from the following components by weight: 5-10 parts of liposome, 0.01-1 part of papain and 0.005-0.5 part of bromelain; wherein the liposome comprises a shell layer and a core layer, the shell layer comprises lecithin and phytosterol, and the core layer comprises camellia sinensis extract and hydrolyzed royal jelly protein; the liposome comprises the following raw materials by weight: 2-15 parts of lecithin, 0.1-5 parts of phytosterol, 0.1-3 parts of camellia sinensis extract, and 0.01-1 part of hydrolyzed royal jelly protein; The preparation method of the liposome comprises the following steps: (1) adding lecithin and phytosterol into an ethanol solution, uniformly mixing, removing ethanol, and obtaining a lipid film; (2) uniformly mixing camellia sinensis extract and hydrolyzed royal jelly protein, adding the lipid film, uniformly stirring at 40-60°C, homogenizing, obtaining the product, filtering out the uncoated components, and obtaining the liposome coated with camellia sinensis extract and hydrolyzed royal jelly protein; wherein the homogenization pressure is 500-800 bar, the homogenization rotation speed is 1000-2000 rpm, and the homogenization time is 3-5 min.

2. The mild exfoliating whitening treatment body care composition of claim 1, wherein The weight ratio of the camellia sinensis extract and the hydrolyzed royal jelly protein is (1.5-3.5):

1.

3. The mild exfoliating whitening treatment body care composition of claim 1, wherein The mass ratio of the liposome, papain and bromelain is 80:(1-5):(0.5-3).

4. Use of the mild exfoliating whitening and repairing body care composition according to any one of claims 1-3 in the preparation of a skin care product.

5. A hand cream, characterized in that, The skin care product comprises the following raw materials by weight percentage: 5%-20% of the mild exfoliating whitening and repairing body care composition according to any one of claims 1-3, 0.1%-0.3% of a thickening agent, 4%-6% of a humectant, 10-13% of oil phase raw materials, 1%-1.5% of a preservative, and 0.1%-0.2% of a pH adjuster, and the balance is deionized water.

6. The hand cream of claim 5, wherein The thickening agent comprises at least one of xanthan gum, carbomer, hydroxyethyl acrylate / acryloyldimethyltaurine sodium copolymer, ammonium acryloyldimethyltaurine / VP copolymer, sordarins, and cetyl alcohol; the humectant comprises at least one of allantoin, sodium polyacrylate, hydrogenated lecithin, betaine, beta-glucan, trehalose, caprylyl glycol, dipropylene glycol, hydrolyzed sodium hyaluronate, 1,2-butanediol, glycerol, short-burst enzyme polysaccharide, and ceramide; the oil phase raw material comprises at least one of cetylstearyl alcohol, caprylic / capric triglyceride, glyceryl stearate citrate, and PEG-100 stearate; the preservative comprises at least one of 1,2-propanediol, 1,2-hexanediol, and p-hydroxyacetophenone; and the pH adjuster comprises at least one of arginine, tromethamine, and disodium EDTA.

7. The method of making the hand cream of claim 6, characterized in that, The method comprises the following steps: S1, uniformly mixing the humectant, the thickening agent, and part of the deionized water, homogenizing into a uniform aqueous phase at 75-85°C, uniformly mixing the oil phase raw materials at 80-90°C, uniformly mixing the aqueous phase and the oil phase raw materials at 75-85°C, and obtaining a mixture; S2, adding preservative into the mixture obtained in step S1, stirring until uniform, when the temperature drops to 35-45℃, adding body care composition and the rest of deionized water, stirring until uniform, finally adding pH regulator to adjust pH, obtaining the hand cream.

Images