Traditional Chinese medicine oral liquid with antihypertensive and refreshing functions and preparation method thereof

Abstract

The invention relates to a traditional Chinese medicine oral liquid with functions of reducing blood pressure and restoring consciousness. The traditional Chinese medicine oral liquid is prepared from the following raw material components in parts by mass: 10-20 parts of ginkgo leaves, 10-20 parts of gastrodia elata, 5-10 parts of selfheal, 5-10 parts of hericium erinaceus, 5-10 parts of a sea-buckthorn extract, 5-15 parts of apocynum venetum, 5-10 parts of a gardenia extract and pumpkin seed peptide. The pumpkin seed peptide comprises 2 to 10 mass percent of pumpkin seed peptide with the molecular weight of less than 4000 Da and 3 to 10 mass percent of pumpkin seed peptide with the molecular weight of 4000 Da to 10000 Da. The traditional Chinese medicine oral liquid with the functions of reducing blood pressure and restoring consciousness can quickly take effect to achieve the purpose of reducing blood pressure aiming at hypertension caused by qi stagnation and blood stasis, and the hypertension is fundamentally treated by adjusting functions of internal organs, promoting blood circulation to remove blood stasis and expanding blood vessels; the refreshing function is achieved by promoting blood circulation to remove blood stasis and transporting qi and nourishing blood, and the memory learning ability is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of Chinese medicine preparation, and in particular to a Chinese medicine oral liquid with blood pressure lowering and brain refreshing functions and a preparation method thereof. Background Art

[0002] As people age, the walls of blood vessels gradually harden, blood flow is obstructed, and blood stasis causes hypertension, arteriosclerosis, as well as cardiovascular and cerebrovascular diseases such as brain atrophy and memory loss. In particular, high blood pressure with a very high risk factor can cause serious problems such as cerebral infarction and cerebral hemorrhage. If high blood pressure persists for a long time, it can cause ischemia and degeneration of cerebral blood vessels, promote cerebral arteriosclerosis, and easily cause cerebral thrombosis. Long-term high blood pressure in the cerebral arteries of hypertensive patients can lead to cerebral arteriosclerosis. Cerebral arteriosclerosis, coupled with spasm and blockage of brain blood vessels, can cause ischemia and hypoxia of the brain, and blood supply will be obstructed, which can cause ischemic necrosis of the brain and form cerebral infarction. This type of disease not only greatly increases the risk of death in the elderly, but also seriously reduces the quality of life of the elderly. The disability rate and mortality rate are very high. It is a common disease for many middle-aged and elderly people. Today, up to 15 million people die from cardiovascular and cerebrovascular diseases every year worldwide, ranking first among all causes of death.

[0003] At present, there are many kinds of Western medicines for the treatment of hypertension, and the effects are remarkable. However, although Western medicines for the treatment of hypertension have the characteristics of rapid effects, they only treat the symptoms and not the root cause. They need to be taken for life, and there are many side effects. Traditional Chinese medicine not only has an important historical position in China, but also still plays an important role in the modern medical system. Traditional Chinese medicine mainly comes from plants, but also includes some animals and minerals. Through different compatibility and processing methods, it is used to prevent and treat various diseases. Traditional Chinese medicine believes that the human body is an organic whole, and the occurrence and development of diseases are the result of the interaction of internal and external factors. Therefore, traditional Chinese medicine emphasizes adjusting the balance of yin and yang and the function of internal organs in the treatment of hypertension to fundamentally solve the problem.

[0004] At present, traditional Chinese medicine mainly treats hypertension of qi stagnation and blood stasis type by promoting blood circulation, dispersing blood stasis, dilating blood vessels, improving blood circulation, organ function, etc. There are many kinds of traditional Chinese medicines for treating hypertension, but most of them have little effect, and are slow to take effect and have poor effect compared with Western medicine. Summary of the invention

[0005] The present invention provides a Chinese medicine oral liquid with the function of lowering blood pressure and refreshing the brain and a preparation method thereof. The Chinese medicine oral liquid can quickly take effect on hypertension caused by qi stagnation and blood stasis to achieve the purpose of lowering blood pressure, and can fundamentally treat hypertension by adjusting the functions of internal organs, promoting blood circulation and removing blood stasis, and dilating blood vessels; and can achieve the function of refreshing the brain by promoting blood circulation and removing blood stasis, promoting qi and nourishing blood, and improving memory and learning ability.

[0006] In order to solve the above technical problems, the technical solution provided by the present invention is:

[0007] A Chinese medicine oral liquid with blood pressure lowering and brain refreshing function, comprising the following raw material components in parts by weight: 10-20 ginkgo leaves, 10-20 gastrodia elata, 5-10 selfheal, 5-10 hericium erinaceus, 5-10 seabuckthorn extract, 5-15 apocynum venetum, 5-10 gardenia extract and pumpkin seed peptide; wherein,

[0008] The pumpkin seed peptides include pumpkin seed peptides with a mass fraction of 2-10 and a molecular weight less than 4000Da, and pumpkin seed peptides with a mass fraction of 3-10 and a molecular weight of 4000Da-10000Da.

[0009] The Chinese medicine oral liquid with blood pressure lowering and brain-awakening function according to the present invention is preferably made of the following raw material components in parts by weight: 1 ginkgo leaf

[0010] According to the Chinese herbal oral liquid with blood pressure lowering and brain refreshing functions described in the present invention, preferably, the pumpkin seed peptide includes a pumpkin seed peptide with a mass fraction of 4 and a molecular weight less than 4000Da, and a pumpkin seed peptide with a mass fraction of 4 and a molecular weight of 4000Da-10000Da.

[0011] In addition, a method for preparing the above-mentioned Chinese medicine oral liquid having the function of lowering blood pressure and refreshing the brain comprises the following steps:

[0012] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed with an ethanol solution containing 20-30wt% of ethanol for 10-30min, and then the steamed mixture is frozen at below 0°C for 2-5h, and then added to water for decoction to obtain a first decoction liquid;

[0013] Step 2: steaming the Hericium erinaceus pieces in water steam for 5-15 minutes, then adding water to the steamed Hericium erinaceus pieces and decocting them twice, each decocting time being 20-60 minutes, and mixing the two decoctions to obtain a second decoction;

[0014] Step 3: dissolve pumpkin seed peptide with a molecular weight of less than 4000Da in water and mix it evenly with sea buckthorn extract, then adjust the pH to 7.5-8.5 and stir for 10-30 minutes to obtain a first solution; dissolve pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mix it evenly with gardenia extract, adjust the pH to 5.5-7 and stir for 10-30 minutes to obtain a second solution;

[0015] Step 4: adding the first decoction into a fermentation tank, adding a mixed bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation to obtain a primary fermentation liquid; adding the second decoction, the first solution and the second solution into the primary fermentation liquid and mixing evenly, then adding a bacterial solution of Bifidobacterium longum for secondary fermentation, sterilizing and filling to obtain a Chinese medicine oral liquid; wherein, the temperature of the primary fermentation is 25-30°C, and the fermentation time is 1-2d; the temperature of the secondary fermentation is 25-30°C, and the fermentation time is 3-4d.

[0016] In the present invention, step three: when preparing the first solution, the water is 2-4 times the total mass of pumpkin seed peptide and sea buckthorn extract; when preparing the second solution, the water is 2-4 times the total mass of pumpkin seed peptide and gardenia extract.

[0017] According to the method of the present invention, preferably, in step 1, the amount of water added is 8-10 times the total mass of the raw materials, the decoction temperature is 95-100°C, and the decoction time is 6-8h.

[0018] According to the method of the present invention, preferably, in step 2, the amount of water added is 8-10 times the mass of the Hericium erinaceus pieces, and the decoction temperature each time is 95-100°C.

[0019] According to the method of the present invention, preferably, in step 2, the second decoction liquid is placed at 90-100° C. for heating treatment, and the heating treatment time is 10-30 min.

[0020] According to the method of the present invention, preferably, in step 4, the composite bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus is prepared by the following method:

[0021] The Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid are mixed in a volume ratio of 1-2:1-2 to obtain a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus.

[0022] According to the method of the present invention, preferably, in step 4, the inoculation amount of the composite bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus is 4-10% (v / v).

[0023] According to the method of the present invention, preferably, in step 4, the inoculation amount of Bifidobacterium longum bacterial solution is 2-3% (v / v).

[0024] The recommended dosage of the traditional Chinese medicine peptide oral liquid prepared by the present invention is 30 mL / person / d.

[0025] According to a specific embodiment of the present invention, the Bacillus subtilis liquid can be prepared by the following steps:

[0026] Take a loop of activated Bacillus subtilis strains, inoculate it into seed culture, and culture it at 37°C, 180r / min for 18 hours to obtain seed liquid; take the seed liquid and inoculate it into fermentation medium, place it in a constant temperature shaker at 30°C and shake culture for 12 hours to obtain Bacillus subtilis liquid, with a rotation speed of 160r / min.

[0027] Seed culture medium: glucose 20g, beef extract 1g, peptone 5g, NaCl 5g, H 2 O1000ml, pH natural, sterilize at 121℃ for 20min.

[0028] Fermentation medium: sucrose 20g, starch 20g, yeast extract 1g, peptone 5g, NaNO 3 3g, KH 2 PO 4 5g, CaCO 3 10g, H 2 O1000ml; pH7.0, sterilize at 105℃ for 15 minutes. The starch is gelatinized starch. The gelatinization method is as follows: add starch to water in a ratio of 1:4 and preheat to 60℃ water, stirring while adding, and boil for 10 minutes to form a paste (stirring continuously during the boiling process).

[0029] According to a specific embodiment of the present invention, the Lactobacillus rhamnosus liquid can be prepared by the following steps:

[0030] The refrigerated Lactobacillus rhamnosus was inoculated into liquid MRS culture medium, and cultured anaerobically at 30-40° C. for 18-24 hours in a constant temperature shaker at 180 rpm / min to obtain a Lactobacillus rhamnosus liquid.

[0031] Liquid MRS medium: casein peptone 10.0 g / L, beef extract 10.0 g / L, yeast extract 5.0 g / L, glucose 20.0 g / L, dipotassium hydrogen phosphate 2.0 g / L, Tween 80 1.0 g / L, triammonium citrate 2.0 g / L, sodium acetate 5.0 g / L, magnesium sulfate 0.1 g / L, manganese sulfate 0.05 g / L, pH 6.5, sterilization at 121°C for 20 min.

[0032] According to a specific embodiment of the present invention, the Bifidobacterium longum bacterial solution can be prepared by the following steps:

[0033] The refrigerated Bifidobacterium longum was inoculated into a BBL agar medium, and anaerobically cultured at 35-37° C. for 72-75 hours at 180 rpm / min in a constant temperature shaker to obtain a Bifidobacterium longum bacterial liquid.

[0034] BBL agar medium: 15g / L peptone, 20g / L glucose, 2g / L yeast extract powder, 0.5g / L soluble starch, 5g / L sodium chloride, 0.5g / L L-cysteine, 5g / L tomato extract powder, 2g / L liver extract powder, 20g / L agar, pH 7.0; mix the above materials with Tween 80 and distilled water (the ratio of each material is 70g:1ml:1000ml), and sterilize at 115°C for 20min.

[0035] Technical effects:

[0036] Compared with the prior art, the Chinese medicine oral liquid with the function of lowering blood pressure and refreshing the brain provided by the present invention is mainly formulated for hypertension caused by qi stagnation and blood stasis. Ginkgo biloba, Gastrodia elata, Prunella vulgaris, Hericium erinaceus, Hippophae rhamnoides extract, Apocynum venetum, Gardenia jasminoides extract, and pumpkin seed peptide are combined in specific amounts, so that the whole prescription is mainly used to promote blood circulation and remove blood stasis, invigorate qi and nourish blood, and has the effects of strengthening the spleen and kidney to achieve the effect of harmonizing yin and yang and regulating the five internal organs, and jointly play the role of lowering blood pressure. In the invention, through the synergistic effect of pumpkin seed peptide and other Chinese medicine components, and through two fermentations of specific strains, more active ingredients can be extracted, which can be quickly absorbed and utilized by the human body, thereby improving the antihypertensive effect.

[0037] The Chinese medicine oral liquid of the present invention can promote blood circulation and dissipate blood stasis, invigorate the mind and refresh the mind, and can also significantly improve cerebral vascular diseases caused by qi stagnation and blood stasis, improve memory and learning ability, and solve the problems of cerebral arteriosclerosis, brain atrophy and confusion, forgetfulness, dementia and the like caused by sequelae of stroke due to qi stagnation and blood stasis and hypertension.

[0038] The Chinese medicine oral liquid with blood pressure lowering and brain refreshing functions provided by the present invention has a reasonable formula, and various medicines are used together, and have the effects of promoting blood circulation and removing blood stasis, promoting qi and nourishing blood, refreshing the mind and the like, thereby achieving rapid blood pressure lowering, refreshing the mind, and treating hypertension by regulating the functions of internal organs. The liquid is easy to carry and convenient to take, and has broad market prospects. DETAILED DESCRIPTION

[0039] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0040] The raw materials and equipment used in the examples and comparative examples of this application are as follows:

[0041] Ginkgo biloba, Gastrodia elata, Prunella vulgaris, Apocynum venetum, and Hericium erinaceus are all produced in China. Seabuckthorn extract, Gardenia jasminoides extract, and pumpkin seed defatted protein powder are all commercially available products, among which the content of geniposide in Gardenia jasminoides extract is 2-4%;

[0042] The constant temperature shaker is the vertical constant temperature shaker HNY-1102C.

[0043] Preparation Example 1 Preparation of pumpkin seed peptide with molecular weight of 4000-10000Da

[0044] This preparation example provides a method for preparing pumpkin seed peptide with a molecular weight of 4000-10000Da, and the steps are as follows:

[0045] 1) Pumpkin seed defatted protein powder is prepared into a 4wt% protein powder aqueous solution, heated and boiled for 15min, and the pH value is adjusted to 6 with a 1mol / L hydrochloric acid aqueous solution, and then a composite enzyme is added at 55°C for reaction for 5h, and then sterilized in boiling water, and the supernatant is centrifuged for standby use; wherein the composite enzyme is obtained by mixing according to an activity unit ratio of 3:1, and the enzyme addition amount is 4000U / mL.

[0046] 2) adjusting the pH value of the supernatant to 6 with a 1 mol / L hydrochloric acid aqueous solution, and then filtering the supernatant with an ultrafiltration membrane having a molecular weight cutoff of 10000Da to obtain a filtrate, and filtering the filtrate with an ultrafiltration membrane having a molecular weight cutoff of 4000Da to obtain a retentate, wherein the operating pressure of both ultrafiltrations is 0.30MPa.

[0047] 3) freeze-drying the retentate to obtain pumpkin seed peptide with a molecular weight of 4000-10000Da.

[0048] Preparation Example 2 Preparation of Pumpkin Seed Peptide with Molecular Weight Less than 4000Da

[0049] This preparation example provides a method for preparing pumpkin seed peptide with a molecular weight less than 4000Da, and the steps are as follows:

[0050] 1) Adding pumpkin seed defatted protein powder into water to prepare a 3wt% protein powder aqueous solution, heating and boiling for 15 minutes, adjusting the pH value of the protein powder aqueous solution to 8, adding Alcalase 2.4L alkaline protease, marine alkaline protease and papain to react at 55°C for 5 hours, sterilizing in boiling water after the reaction, centrifuging and taking the supernatant for later use; wherein Alcalase 2.4L alkaline protease, marine alkaline protease and papain are mixed in an activity unit ratio of 3:3:1, and the enzyme addition amount is 4000U / mL.

[0051] 2) The pH value of the supernatant was adjusted to 7.5 with 1 wt % sodium hydroxide solution, and then filtered through an ultrafiltration membrane with a molecular weight cutoff of 4000 Da to obtain a filtrate, wherein the operating pressure of the ultrafiltration was 0.25 MPa.

[0052] 3) freeze-drying the filtrate to obtain pumpkin seed peptide powder with a molecular weight of less than 4000Da.

[0053] Example 1

[0054] The present embodiment provides a Chinese medicine oral liquid with antihypertensive function, comprising the following raw material components in parts by weight: 15 ginkgo leaves, 12 gastrodia elata, 8 selfheal, 8 hericium erinaceus, 8 seabuckthorn extract, 12 rhubarb, and 6 gardenia extract; 4 pumpkin seed peptides with a molecular weight of less than 4000Da, and 4 pumpkin seed peptides with a molecular weight of 4000Da-10000Da.

[0055] The preparation method of the above-mentioned Chinese medicine liquid comprises the following steps:

[0056] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0057] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0058] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0059] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0060] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as Y1. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0061] Example 2

[0062] The present embodiment provides a Chinese medicine oral liquid with antihypertensive function, comprising the following raw material components in parts by weight: 10 ginkgo leaves, 10 gastrodia elata, 5 selfheal, 10 hericium erinaceus, 10 seabuckthorn extract, 10 dogbane, and 10 gardenia extract; 2 pumpkin seed peptides with a molecular weight of less than 4000Da, and 3 pumpkin seed peptides with a molecular weight of 4000Da-10000Da.

[0063] The preparation method of the above-mentioned Chinese medicine oral liquid comprises the following steps:

[0064] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 15 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 2 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0065] Step 2: steaming the Hericium erinaceus pieces in water steam for 15 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0066] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0067] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0068] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as Y2. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0069] Example 3

[0070] The present embodiment provides a Chinese medicine oral liquid with antihypertensive function, comprising the following raw material components in parts by weight: 20 ginkgo leaves, 18 gastrodia elata, 10 selfheal, 10 hericium erinaceus, 10 seabuckthorn extract, 10 dogbane, 10 gardenia extract; 10 pumpkin seed peptides with a molecular weight of less than 4000Da, and 10 pumpkin seed peptides with a molecular weight of 4000Da-10000Da.

[0071] The preparation method of the above-mentioned Chinese medicine oral liquid comprises the following steps:

[0072] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 2-5 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0073] Step 2: steaming the Hericium erinaceus pieces in water steam for 5 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0074] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0075] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are combined in a volume ratio of 1:2, and the inoculation amount of the composite bacterial liquid is 10% (v / v);

[0076] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as Y3. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0077] Example 4

[0078] The present embodiment provides a Chinese medicine oral liquid with antihypertensive function, comprising the following raw material components in parts by weight: 15 ginkgo leaves, 12 gastrodia elata, 8 selfheal, 8 hericium erinaceus, 8 seabuckthorn extract, 12 rhubarb, and 6 gardenia extract; 4 pumpkin seed peptides with a molecular weight of less than 4000Da, and 4 pumpkin seed peptides with a molecular weight of 4000Da-10000Da.

[0079] The preparation method of the above-mentioned Chinese medicine oral liquid comprises the following steps:

[0080] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 2-5 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0081] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0082] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0083] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are combined in a volume ratio of 2:1, and the inoculation amount of the composite bacterial liquid is 4% (v / v);

[0084] The second decoction, the first solution and the second solution are added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid is added for secondary fermentation. The temperature of the secondary fermentation is 30°C and the fermentation time is 3 days. The Chinese medicine oral liquid is sterilized and filled, and marked as Y4; the inoculation amount of Bifidobacterium longum liquid is 2.5% (v / v).

[0085] Comparative Example 1

[0086] The raw material components of this comparative example are the same as those of Example 1 except that Prunella vulgaris is not included, and the preparation method is as follows:

[0087] Step 1: Ginkgo biloba leaves, Gastrodia elata and Apocynum venetum are weighed and crushed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture for decoction to obtain a first decoction;

[0088] Step 2: steaming the Hericium erinaceus pieces in water steam for 15 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0089] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0090] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0091] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as D1. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0092] Comparative Example 2

[0093] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0094] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, Apocynum venetum and Hericium erinaceus pieces are weighed and crushed and mixed according to a proportion, the mixture is steamed with an ethanol solution containing 20wt% ethanol for 20min, and then the steamed mixture is frozen at -5°C for 3h, and then added into water 8 times the mass of the mixture for decoction to obtain a first decoction liquid;

[0095] Step 2: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0096] Step 3: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0097] The first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C, the fermentation time was 3 days, and the Chinese medicine oral liquid was sterilized and filled, marked as D2; the inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0098] Comparative Example 3

[0099] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0100] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0101] Step 2: steaming the Hericium erinaceus pieces in water steam for 30 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0102] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0103] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0104] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as D3. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0105] Comparative Example 4

[0106] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0107] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is added into water of 8 times the mass of the mixture and boiled to obtain a first decoction;

[0108] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0109] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0110] Step 4: adding the first decoction liquid to a fermentation tank, adding a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation, wherein the temperature of the primary fermentation is 30° C., the fermentation time is 1 day, and a primary fermentation liquid is obtained; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial liquid are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial liquid is 6% (v / v);

[0111] The second decoction, the first solution and the second solution were added to the primary fermentation liquid and mixed evenly, and then Bifidobacterium longum liquid was added for secondary fermentation. The temperature of the secondary fermentation was 30°C and the fermentation time was 3 days. The Chinese medicine oral liquid was sterilized and filled, and marked as D4. The inoculation amount of Bifidobacterium longum liquid was 2.5% (v / v).

[0112] Comparative Example 5

[0113] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0114] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0115] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0116] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0117] Step 4: Add the first decoction, the second decoction, the first solution, and the second solution to a fermentation tank, first add a mixed bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus for a fermentation, the temperature of the first fermentation is 30°C, the fermentation time is 1d, and a primary fermentation liquid is obtained, and then a long bifidobacterium bacterial solution is added for a secondary fermentation, the temperature of the secondary fermentation is 30°C, the fermentation time is 3d, sterilization, and filling to obtain a Chinese medicine oral liquid, marked as D5; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the mixed bacterial solution are mixed in a volume ratio of 1:1, and the inoculation amount of the mixed bacterial solution is 6% (v / v); the inoculation amount of the long bifidobacterium liquid is 2.5% (v / v).

[0118] Comparative Example 6

[0119] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0120] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0121] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0122] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0123] Step 4: Add the first decoction, the second decoction, the first solution, and the second solution into a fermentation tank, add a composite bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus, and a Bifidobacterium longum bacterial solution for fermentation, the temperature of the first fermentation is 30° C., the fermentation time is 3 days, sterilize, and fill to obtain a Chinese medicine oral liquid, marked as D6; the Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid in the composite bacterial solution are mixed in a volume ratio of 1:1, and the inoculation amount of the composite bacterial solution is 6% (v / v); the inoculation amount of the Bifidobacterium longum liquid is 2.5% (v / v).

[0124] Comparative Example 7

[0125] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0126] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is frozen at -5°C for 3 hours, and then added into water 8 times the mass of the mixture and boiled to obtain a first decoction;

[0127] Step 2: steaming the Hericium erinaceus pieces in water steam for 10 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 30 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0128] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0129] Step 4: Mix the first decoction, the second decoction, the first solution and the second solution and fill them to obtain a Chinese medicine oral liquid, marked as D7.

[0130] Comparative Example 8

[0131] The raw material components of this comparative example are the same as those of Example 1, and the preparation method is as follows:

[0132] Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed for 20 minutes with an ethanol solution containing 20 wt% of ethanol, and then the steamed mixture is added into water of 8 times the mass of the mixture and boiled to obtain a first decoction;

[0133] Step 2: steaming the Hericium erinaceus pieces in water steam for 30 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling them twice, each boiling time is 10 minutes, the mass of water added each time is 8 times the mass of the Hericium erinaceus pieces, and mixing the two decoctions to obtain a second decoction;

[0134] Step 3: dissolving a pumpkin seed peptide with a molecular weight of less than 4000Da in water and mixing it evenly with the seabuckthorn extract, then adjusting the pH to 8 and stirring for 30 minutes to obtain a first solution; dissolving a pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mixing it evenly with the gardenia extract, adjusting the pH to 6 and stirring for 30 minutes to obtain a second solution; when preparing the first solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the seabuckthorn extract; when preparing the second solution, the amount of water is 3 times the total mass of the pumpkin seed peptide and the gardenia extract;

[0135] Step 4: adding the first decoction into a fermentation tank, adding Lactobacillus rhamnosus liquid for primary fermentation, the temperature of the primary fermentation is 30°C, the fermentation time is 1 day, and the primary fermentation liquid is obtained; the inoculation amount of the Lactobacillus rhamnosus liquid is 6% (v / v); adding the second decoction, the first solution, and the second solution into the primary fermentation liquid and mixing evenly, then adding Bifidobacterium longum liquid for secondary fermentation, the temperature of the secondary fermentation is 30°C, the fermentation time is 3 days, sterilizing and filling to obtain the Chinese medicine oral liquid, marked as D8; the inoculation amount of the Bifidobacterium longum liquid is 2.5% (v / v).

[0136] Experimental Example 1 Behavioral influence experiment

[0137] 150 healthy male SD rats were taken and randomly divided into a blank group, a model group, a control group, an embodiment 1-4 group, and a comparative example 1-8 group, with 10 rats in each group. Except for the blank group, rats in other groups were intraperitoneally injected with 0.5wt% D-galactose at a dose of 50mg / kg, once a day, for 6 consecutive weeks to establish a dementia model. Rats in the embodiment 1-4 group and the comparative example 1-8 group were gavaged with the Chinese medicine oral liquid prepared in the embodiment 1-4 and the comparative example 1-8 at the beginning of the 6th week, and were all administered at 10 times the human body per kg dosage, once a day, for 4 consecutive weeks. The control group was gavaged with 0.6g / kg / d of Naofukang, and the blank group and the model group were gavaged with an equal amount of normal saline. Learning and memory improvement effect, the results are shown in Table 1.

[0138] (1) Positioning navigation test: One day before the test, the rats were allowed to swim freely in the pool for 2 minutes. The test lasted for 5 days, and the time it took to find the platform each time (escape latency) was recorded. If the rats could not find the platform within 120 seconds, they were led to the platform, and the latency was recorded as 120 seconds;

[0139] (2) Spatial exploration test: On the afternoon of the fifth day, the platform was removed and the swimming path and time of the rats in the pool were recorded within 2 min, including the swimming time in the quadrant of the original platform and the swimming time in the 40 cm ring centered on the platform.

[0140] Table 1

[0141] Group Escape latency(s) Original platform quadrant swimming time (s) Swimming time in 40cm ring (s) Blank group 26.18±15.23 67.13±10.21 15.79±1.23 Model Group 42.35±16.26 26.55±9.42 2.01±2.03 Control group 34.75±17.03 51.22±11.23 9.76±1.53 Example 1 Group 28.10±14.55 61.19±12.04 13.04±2.34 Example 2 Group 30.89±13.63 59.31±9.11 12.13±1.19 Example 3 Group 29.12±16.14 60.34±8.48 12.68±1.91 Example 4 Group 28.60±17.51 59.98±10.05 12.38±1.37 Comparative Example 1 36.22±14.11 47.28±9.43 7.65±2.21 Comparative Example 2 32.08±18.01 53.09±9.61 8.49±1.59 Comparative Example 3 28.07±12.35 62.01±9.15 13.19±2.06 Comparative Example 4 31.49±16.48 55.14±10.22 10.04±1.34 Comparative Example 5 30.81±15.02 56.42±12.05 11.39±2.31 Comparative Example 6 31.59±16.10 55.66±9.47 10.28±1.87 Comparative Example 7 34.22±12.13 48.53±10.23 8.54±1.27 Comparative Example 8 31.67±13.51 54.91±11.29 11.76±1.40

[0142] Batch

[0143] Experimental Example 2 Animal Hypotension Test

[0144] 150 normal healthy rabbits were randomly selected and divided into a blank group, a model group, a control group, an embodiment 1-4 group, and a comparative example 1-8 group, with 10 rabbits in each group, half male and half female. 140 rabbits were randomly selected to establish a hypertension model and divided into 14 groups, namely, the embodiment 1-4 group, the comparative example 1-8 group, the model group, and the control group. The experimental results after grouping are shown in Table 2 below:

[0145] Table 2 Grouping and dosage

[0146] Grouping Feeding medicine dose Blank group Normal saline 20mL / piece / day Model Group Normal saline 20mL / piece / day Control group Captopril 10mg / Kg Example 1 Group Y1 20mL / piece / day Example 2 Group Y2 20mL / piece / day Example 3 Group Y3 20mL / piece / day Example 4 Group Y4 20mL / piece / day Comparative Example 1 D1 20mL / piece / day Comparative Example 2 D2 20mL / piece / day Comparative Example 3 D3 20mL / piece / day Comparative Example 4 D4 20mL / piece / day Comparative Example 5 D5 20mL / piece / day Comparative Example 6 D6 20mL / piece / day Comparative Example 7 D7 20mL / piece / day Comparative Example 8 D8 20mL / piece / day

[0147] The drugs were administered by oral gavage according to the dosage in Table 2, and the blood pressure values ​​of the rabbits in each test group were observed over time within 24 hours. The blood pressure values ​​of the rabbits in each test group were measured every 4 hours, and 5 data were taken each time. The average value was finally taken. The results of the blood pressure value changes in each group are shown in Table 3.

[0148] Table 3 Experimental results

[0149]

[0150] From the results in Tables 1-3, we can draw the following conclusions:

[0151] Compared with the rats in the model group, the learning and memory of the rats in the Example 1-4 group were significantly improved; compared with the control group taking Naofukang, the memory of the rats in the Example 1-4 group was also greatly improved, indicating that the oral liquid of the present invention has the effect of improving memory. The blood pressure of the rabbits in Example 1-4 can drop rapidly within 4 hours, and is basically stable within 24 hours, with only a small upward trend in the later period, indicating that the Chinese medicine oral liquid of the present invention has the effect of quickly controlling blood pressure; compared with the control group, the blood pressure control effect within 12 hours is slightly worse. Due to the characteristics of Chinese medicine that fundamentally promotes blood circulation and removes blood stasis and nourishes the five internal organs, compared with the control group, the Example 1-4 group still has a good blood pressure control effect after 12 hours. The control group only treats the symptoms but not the root cause. After 24 hours, the blood pressure returns to the level before taking the medicine, and the long-term blood pressure control effect is significantly worse than that of the Chinese medicine oral liquid of the present invention.

[0152] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modification, equivalent substitution or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A Chinese medicine oral liquid with blood pressure lowering and brain refreshing functions, characterized in that: The invention comprises the following raw material components in parts by weight: 10-20 ginkgo leaves, 10-20 gastrodia elata, 5-10 selfheal, 5-10 hericium erinaceus, 5-10 seabuckthorn extract, 5-15 apocynum venetum, 5-10 gardenia extract and pumpkin seed peptide; wherein, The pumpkin seed peptides include pumpkin seed peptides with a mass fraction of 2-10 and a molecular weight less than 4000Da, and pumpkin seed peptides with a mass fraction of 3-10 and a molecular weight of 4000Da-10000Da.

2. The Chinese medicine oral liquid with blood pressure lowering and brain refreshing function as claimed in claim 1, characterized in that: The invention is prepared from the following raw material components in parts by weight: 15 ginkgo leaves, 12 gastrodia elata, 8 selfheal, 8 hericium erinaceus, 8 seabuckthorn extract, 12 apocynum venetum and 6 gardenia extract.

3. A Chinese medicine oral liquid with blood pressure lowering and brain-awakening function as claimed in claim 1 or 2, characterized in that: The pumpkin seed peptide includes a pumpkin seed peptide with a mass fraction of 4 and a molecular weight less than 4000Da, and a pumpkin seed peptide with a mass fraction of 4 and a molecular weight of 4000Da-10000Da.

4. A method for preparing a Chinese medicine oral liquid having the function of lowering blood pressure and refreshing the brain as claimed in claim 1, characterized in that: The following steps are involved: Step 1: Ginkgo biloba leaves, Gastrodia elata, Prunella vulgaris, and Apocynum venetum are weighed and crushed and mixed according to a proportion, the mixture is steamed with an ethanol solution containing 20-30wt% of ethanol for 10-30min, and then the steamed mixture is frozen at below 0°C for 2-5h, and then added to water for decoction to obtain a first decoction liquid; Step 2: steaming the Hericium erinaceus pieces in water steam for 5-15 minutes, then adding the steamed Hericium erinaceus pieces into water and boiling twice, each boiling time being 20-60 minutes, and mixing the two decoctions to obtain a second decoction; Step 3: dissolve pumpkin seed peptide with a molecular weight of less than 4000Da in water and mix it evenly with sea buckthorn extract, then adjust the pH to 7.5-8.5 and stir for 10-30 minutes to obtain a first solution; dissolve pumpkin seed peptide with a molecular weight of 4000Da-10000Da in water and mix it evenly with gardenia extract, adjust the pH to 5.5-7 and stir for 10-30 minutes to obtain a second solution; Step 4: adding the first decoction into a fermentation tank, adding a mixed bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus for primary fermentation to obtain a primary fermentation liquid; adding the second decoction, the first solution and the second solution into the primary fermentation liquid and mixing evenly, then adding a bacterial solution of Bifidobacterium longum for secondary fermentation, sterilizing and filling to obtain a Chinese medicine oral liquid; wherein, the temperature of the primary fermentation is 25-30°C, and the fermentation time is 1-2d; the temperature of the secondary fermentation is 25-30°C, and the fermentation time is 3-4d.

5. A method according to claim 4, characterized in that: In step 1, the amount of water added is 8-10 times the total mass of the raw materials, the decoction temperature is 95-100° C., and the decoction time is 6-8 hours.

6. A method according to claim 4, characterized in that: In step 2, the amount of water added is 8-10 times the mass of the Hericium erinaceus pieces, and the decoction temperature is 95-100° C. each time.

7. A method according to claim 4, characterized in that: In step 2, the second decoction liquid is heated at 90-100° C. for 10-30 minutes.

8. A method according to claim 4, characterized in that: In step 4, the composite bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus is prepared by the following method: The Bacillus subtilis liquid and the Lactobacillus rhamnosus liquid are mixed in a volume ratio of 1-2:1-2 to obtain a composite bacterial liquid of Bacillus subtilis and Lactobacillus rhamnosus.

9. A method according to claim 4, characterized in that: In step 4, the inoculation amount of the mixed bacterial solution of Bacillus subtilis and Lactobacillus rhamnosus is 4-10% (v / v).

10. The method according to claim 4, characterized in that: In step 4, the inoculation amount of Bifidobacterium longum bacterial solution is 2-3% (v / v).

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