Cell centrifugal subpackaging device and method

Abstract

The invention relates to the technical field of cell centrifugation, and discloses a cell centrifugal subpackaging device and method.The cell centrifugal subpackaging device comprises a base, a sleeve is rotationally installed on the upper surface of the base, a rotating disc is fixedly installed on the circumferential outer surface, close to the top end, of the sleeve, and a plurality of openings are formed in the circumferential outer surface of the rotating disc at equal intervals in a penetrating mode; two rotating columns are symmetrically and rotationally mounted on the inner walls of the two opposite sides of each opening, and a test tube sleeve is fixedly mounted between the adjacent ends of the two rotating columns. After centrifugation is finished, the rotating disc stops rotating, at the moment, the test tube sleeves return to the vertical positions, meanwhile, the arc clamps drive the multiple rubber clamping wheels in the arc clamps to leave the surfaces of the centrifugal test tubes, one-by-one manual operation is not needed when limiting of the centrifugal test tubes is relieved through the device, the workload of operators is reduced when cell centrifugal subpackaging is conducted in batches, and the working efficiency is improved. And the efficiency of cell centrifugal subpackaging is improved.

Description

Technical Field

[0001] The invention relates to the technical field of cell centrifugation, and in particular to a cell centrifugation packaging device and method. Background Art

[0002] The cell centrifugal filling device is an auxiliary device specially used for sterile experimental operations. It usually includes a centrifuge, centrifuge tubes, a filling device and other necessary components to achieve solid-liquid separation and filling of cell cultures. The centrifuge is the core component of the cell centrifugal filling device. The centrifuge generates centrifugal force through high-speed rotation, which allows different components in cell cultures to be separated.

[0003] An existing cell centrifugal packaging device (Announcement No.: CN222019764U) has at least the following disadvantages: When the above patent is in use, the fixing mechanism is set to fix the centrifuge tube near the entrance of the placement groove after the staff puts the centrifuge tube into the placement groove, and the separation mechanism is set to clamp and fix the bottom end of the centrifuge tube after the centrifuge tube is inserted, thereby preventing the centrifuge tube from shaking during the centrifugation process. The above patent fixes the test tube by setting a fixing mechanism and a separation mechanism, but after the centrifugation work is completed, the fixing mechanism and the separation mechanism need to be manually released one by one to fix the test tube. When batch cell centrifugation packaging is performed, the workload of the operator will be greatly increased, resulting in a decrease in the efficiency of cell centrifugation packaging. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a cell centrifugal packaging device and method.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions: The cam is provided with a plurality of springs, each of which has a plurality of openings equidistant from the top of the cam, and two or more rotating columns are symmetrically and rotatably mounted on the inner walls of the plurality of openings on opposite sides. A test tube sleeve is fixedly and mounted between adjacent ends of the two rotating columns. A centrifugal test tube is inserted into the interior of the test tube sleeve. Two supporting blocks are symmetrically and fixedly mounted on the end surfaces of the top ends of the test tube sleeve. A top column is slidably inserted into the inner walls of the two supporting blocks. An arc clamp is fixedly and mounted on the outer surface of the supporting block at one adjacent end of the top column. An installation groove is provided on the outer surface of the arc clamp. A plurality of rubber clamp wheels are equidistantly and rotatably mounted between the inner walls of the installation groove. The plurality of rubber clamp wheels abut against the circumferential outer surface of the centrifugal test tube. An arc groove is provided on the inner walls of the opening on opposite sides. The top column is inserted into the interior of the arc groove and slidably mounted on the inner wall of the arc groove.

[0006] As a further solution of the present invention, a retaining ring is fixedly installed on the outer surface of the top column close to the arc groove, and a second spring is sleeved on the circumferential outer surface of the top column. The second spring is arranged between the support block and the retaining ring. The inner wall of the arc groove close to the support block is provided with an arc bevel, and the end face of the top column is provided with a chamfer matching the arc bevel. The chamfer of the end face of the top column offsets the arc bevel, and the arc groove and the rotation center of the rotating column are arranged at the same center.

[0007] As a further solution of the present invention, a fixing column is fixedly installed on the upper surface of the base, and the fixing column is arranged inside the sleeve. The top end of the fixing column passes through the upper surface of the turntable and a fixing disk is fixedly installed thereon. A circular groove is provided on the upper surface of the turntable, and the fixing disk is arranged inside the circular groove. A driving chamfer is provided on the circumferential outer surface of the fixing disk near the top end, and a fourth gear is fixedly installed on the top end of the rubber clamp wheel near the fixed disk and passes through the upper surface of the arc clamp.

[0008] As a further solution of the present invention, a third gear is rotatably mounted on the circumferential outer surface of the arc clamp, the third gear is meshed with a fourth gear, a rubber friction wheel is fixedly mounted on the upper surface of the middle position of the third gear, the circumferential outer surface of the rubber friction wheel is spherically arranged, and the circumferential outer surface of the rubber friction wheel is against the outer surface of the driving chamfer.

[0009] As a further solution of the present invention, a first gear is fixedly installed on the circumferential outer surface of the sleeve near the bottom end, a second gear is rotatably installed on the upper surface of the base, the second gear is meshed with the first gear, and a driving motor is fixedly installed on the lower surface of the base, and the output end of the driving motor passes through the upper surface of the base and is fixedly installed at the rotation center of the second gear.

[0010] As a further solution of the present invention, a plurality of grooves are equidistantly provided in the circumferential direction of the lower surface of the turntable, and slide plates are slidably installed on the inner walls of the plurality of grooves, a dovetail groove is provided on the top wall of the groove, and a dovetail slide rail matching the dovetail groove is provided on the upper surface of the slide plate, and the dovetail slide rail is slidably installed on the inner wall of the dovetail slide groove, and arc-shaped slide grooves are penetrated through the inner walls of the opposite ends of the groove, and plug blocks are slidably installed on the inner walls of the arc-shaped slide grooves.

[0011] As a further solution of the present invention, two insertion holes are symmetrically provided on the circumferential outer surface of the test tube sleeve, one end of the insertion block is inserted into the inside of the insertion hole, a driving column is fixedly installed on the upper surface of the other end of the insertion block, two driving grooves are symmetrically penetrated through the upper surface of the slide plate, the driving column is slidably installed on the inner wall of the driving groove, a first spring is fixedly installed on the outer surface of the slide plate away from the sleeve, and the other end of the first spring is fixedly connected to the inner wall of the sink groove.

[0012] As a further solution of the present invention, an X-direction moving component is slidably installed between the outer surfaces on opposite sides of the base, a Y-direction moving component is arranged on the top of the X-direction moving component, a Z-direction moving component is arranged on the outer surface of one side of the Y-direction moving component, and a dispensing needle is arranged on the bottom of the Z-direction moving component.

[0013] A method for using a cell centrifugal packaging device comprises the following steps: S1: When in use, the operator first inserts the centrifugal test tube containing the cell sample into the test tube sleeve, and then starts the driving motor, which drives the turntable to rotate. The rotation of the turntable causes the slide plate to slide outward under the action of centrifugal force, and the slide plate drives the plug block to retract into the sink through the driving groove and the driving column, and the plug block leaves the insertion hole. At this time, the test tube sleeve is released from the limit; S2: The tilting movement of the test tube sleeve drives the rubber friction wheel to counteract the driving chamfer of the fixed plate. At this time, since the rotating plate and the fixed plate are in relative motion, the rubber friction wheel rotates by friction with the driving chamfer, and drives one of the rubber clamping wheels to rotate through the rubber friction wheel, and the rubber clamping wheel drives the centrifugal test tube to rotate. S3: The test tube sleeve is driven to move by the turntable, and the centrifugal test tube is driven to move by the test tube sleeve. Due to the centrifugal force, the test tube sleeve will tilt 30° along the direction of the arc groove. The sample inside the centrifugal test tube is subjected to the centrifugal force to separate cells or cell components of different densities from the liquid; S4: After the centrifugation is completed, the X-axis moving component, the Y-axis moving component and the Z-axis moving component drive the packaging needle to move to the top of the designated centrifuge tube, extract the centrifuged cell fluid inside the centrifuge tube in layers, and package it for subsequent testing.

[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. After the centrifugation is completed, the turntable stops rotating. At this time, the test tube sleeve returns to the vertical position. At the same time, the arc clamp drives the internal multiple rubber clamp wheels to leave the surface of the centrifugal test tube. When the centrifugal test tube is released from the limit by this device, there is no need to operate manually one by one. When batch cell centrifugation is performed, the workload of the operator is reduced and the efficiency of cell centrifugation is accelerated; 2. The tilting movement of the test tube sleeve drives the rubber friction wheel to counteract the driving chamfer of the fixed disk. At this time, since the turntable and the fixed disk are in relative motion, the rubber friction wheel rotates itself through the friction with the driving chamfer, and the rubber friction wheel drives one of the rubber clamping wheels to rotate, and the rubber clamping wheel drives the centrifugal test tube to rotate. The periodic centrifugal force changes generated by the rotation of the centrifugal test tube can slightly disturb the sedimentation, avoiding the formation of hard knots of cells or particles at the bottom of the centrifugal test tube, which is convenient for subsequent resuspension. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the overall structure of a cell centrifugal packaging device proposed by the present invention; Figure 2 This is a bottom view schematic diagram of the structure of a cell centrifugal packaging device proposed by the present invention; Figure 3 A schematic diagram of the top view of the turntable of a cell centrifugal packaging device proposed by the present invention; Figure 4 This is a schematic diagram of the structure of a turntable of a cell centrifugal packaging device proposed by the present invention when viewed from above; Figure 5 A schematic diagram of a fixed plate of a cell centrifugal packaging device proposed by the present invention; Figure 6 A schematic diagram of a slide plate of a cell centrifugal packaging device proposed by the present invention; Figure 7 A schematic diagram of a test tube sleeve of a cell centrifugal packaging device proposed by the present invention; Figure 8 A schematic diagram of an arc clamp of a cell centrifugal packaging device proposed by the present invention; Fig. 9 for Figure 5 A partial enlarged schematic diagram in the middle; Fig.10 for Figure 4 A partial enlarged schematic diagram of point B in the middle.

[0016] In the figure: 1, base; 2, X-axis moving assembly; 3, Y-axis moving assembly; 4, Z-axis moving assembly; 5, dispensing needle; 6, sleeve; 7, first gear; 8, second gear; 9, driving motor; 10, fixed plate; 1001, driving chamfer; 1002, fixed column; 11, turntable; 12, test tube sleeve; 1201, support block; 1202, rotating column; 1203, socket; 13, circular groove; 14, opening; 15, sinking groove; 16, dovetail slide; 17, slide plate; 18, first spring; 19, plug block; 20, driving groove; 21, driving column; 22, arc clamp; 23, mounting groove; 24, rubber clamp wheel; 25, top column; 26, retaining ring; 27, second spring; 28, rubber friction wheel; 29, third gear; 30, fourth gear; 31, arc groove. DETAILED DESCRIPTION

[0017] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0018] In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "two ends", "one end", "the other end" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first" and "second" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0019] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0020] Reference Figure 1-Figure 10 A cell centrifugal packaging device comprises a base 1, a sleeve 6 is rotatably mounted on the upper surface of the base 1, a turntable 11 is fixedly mounted on the circumferential outer surface of the sleeve 6 near the top, a plurality of openings 14 are equidistantly penetrated through the circumferential outer surface of the turntable 11, two rotating columns 1202 are symmetrically rotatably mounted on the inner walls on opposite sides of the plurality of openings 14, a test tube sleeve 12 is fixedly mounted between adjacent ends of the two rotating columns 1202, a centrifugal test tube is inserted inside the test tube sleeve 12, two support blocks 1201 are symmetrically fixedly mounted on the end surface of the top of the test tube sleeve 12, top columns 25 are slidably inserted on the inner walls of the two support blocks 1201, an arc clamp 22 is fixedly mounted on the adjacent end of the top column 25 penetrating through the outer surface of the support block 1201, and a mounting groove 23 is opened on the outer surface of the arc clamp 22, A plurality of rubber clamping wheels 24 are equidistantly installed between the inner walls of the mounting groove 23 for rotation, and the plurality of rubber clamping wheels 24 are against the circumferential outer surface of the centrifuge test tube. Circular arc grooves 31 are provided on the inner walls on opposite sides of the opening 14, and a top column 25 is inserted into the inside of the circular arc groove 31 and slidably installed with the inner wall thereof. A retaining ring 26 is fixedly installed on the outer surface of the top column 25 close to the circular arc groove 31, and a second spring 27 is sleeved on the circumferential outer surface of the top column 25, and the second spring 27 is arranged between the support block 1201 and the retaining ring 26. An arc bevel is provided on the inner wall of the arc groove 31 close to the support block 1201, and a chamfer matching the arc bevel is provided on the end face of the top column 25, and the chamfer on the end face of the top column 25 is against the arc bevel, and the arc groove 31 and the rotation center of the rotating column 1202 are arranged at the same center.

[0021] The tilting movement of the test tube sleeve 12 allows the top column 25 to slide on the inner wall of the arc groove 31. When the chamfered end face of the top column 25 slides relative to the arc bevel, it will drive the two arc clamps 22 to move closer to the centrifuge test tube. The arc clamps 22 drive the internal multiple rubber clamping wheels 24 to press against the outer surface of the centrifuge test tube. The centrifuge test tube is limited by the device to avoid shaking during centrifugal movement. After the centrifugation is completed, the turntable 11 stops rotating. At this time, the test tube sleeve 12 returns to the vertical position. At the same time, the arc clamps 22 drive the internal multiple rubber clamping wheels 24 to leave the surface of the centrifuge test tube. When the centrifuge test tube is released from the limit by the device, there is no need to manually operate one by one. When batch cell centrifugation is performed, the workload of the operator is reduced and the efficiency of cell centrifugation is accelerated.

[0022] In this embodiment, a fixing column 1002 is fixedly installed on the upper surface of the base 1, and the fixing column 1002 is arranged inside the sleeve 6. The top end of the fixing column 1002 passes through the upper surface of the turntable 11 and a fixing disk 10 is fixedly installed thereon. A circular groove 13 is provided on the upper surface of the turntable 11, and the fixing disk 10 is arranged inside the circular groove 13. A driving chamfer 1001 is provided on the circumferential outer surface of the fixing disk 10 near the top. The top end of the rubber clamping wheel 24 near the fixed disk 10 passes through the upper surface of the arc clamp 22 and a fourth gear 30 is fixedly installed thereon. A third gear 29 is rotatably installed on the circumferential outer surface of the arc clamp 22, and the third gear 29 is meshed with the fourth gear 30. A rubber friction wheel 28 is fixedly installed on the upper surface at the middle position of the third gear 29, and the circumferential outer surface of the rubber friction wheel 28 is spherically arranged, and the circumferential outer surface of the rubber friction wheel 28 is against the outer surface of the driving chamfer 1001.

[0023] The tilting movement of the test tube sleeve 12 drives the rubber friction wheel 28 to abut against the driving chamfer 1001 of the fixed disk 10. At this time, since the turntable 11 and the fixed disk 10 are in relative motion, the rubber friction wheel 28 rotates itself through the friction with the driving chamfer 1001, and the rubber friction wheel 28 drives one of the rubber clamping wheels 24 to rotate, and the rubber clamping wheel 24 drives the centrifugal test tube to rotate. The periodic centrifugal force changes generated by the rotation of the centrifugal test tube can slightly disturb the sedimentation, thereby preventing cells or particles from forming a lump at the bottom of the centrifugal test tube, which is convenient for subsequent resuspension.

[0024] In this embodiment, a first gear 7 is fixedly installed on the circumferential outer surface of the sleeve 6 near the bottom end, a second gear 8 is rotatably installed on the upper surface of the base 1, the second gear 8 is meshed with the first gear 7, and a drive motor 9 is fixedly installed on the lower surface of the base 1. The output end of the drive motor 9 passes through the upper surface of the base 1 and is fixedly installed at the rotation center of the second gear 8.

[0025] The second gear 8 is driven to rotate by the driving motor 9, and the second gear 8 drives the sleeve 6 to rotate through the first gear 7, and the sleeve 6 drives the turntable 11 to rotate, and the turntable 11 drives the test tube sleeve 12 to move, and the test tube sleeve 12 drives the centrifugal test tube to move. Due to the effect of centrifugal force, the test tube sleeve 12 will tilt at an angle of 30° along the direction of the arc groove 31. At this time, the sample inside the centrifugal test tube is subjected to the centrifugal force to separate cells or cell components of different densities from the liquid. The device is convenient for centrifugal separation of cell samples.

[0026] In this embodiment, a plurality of sink grooves 15 are equidistantly provided in the circumferential direction of the lower surface of the turntable 11, and slide plates 17 are slidably installed on the inner walls of the plurality of sink grooves 15, and a dovetail slide groove 16 is provided on the top wall of the sink groove 15, and a dovetail slide rail matching the dovetail slide groove 16 is provided on the upper surface of the slide plate 17, and the dovetail slide rail is slidably installed with the inner wall of the dovetail slide groove 16, and the inner walls of the opposite ends of the sink groove 15 are penetrated with arc-shaped slide grooves, and an insert block 19 is slidably installed on the inner wall of the arc-shaped slide groove, and the circumferential outer surface of the test tube sleeve 12 is symmetrically provided with two insertion holes 1203, one end of the insert block 19 is inserted into the inside of the insertion hole 1203, and a driving column 21 is fixedly installed on the upper surface of the other end of the insert block 19, and two driving grooves 20 are symmetrically penetrated on the upper surface of the slide plate 17, and the driving column 21 is slidably installed with the inner wall of the driving groove 20, and the outer surface of the slide plate 17 away from the sleeve 6 is fixedly provided with a first spring 18, and the other end of the first spring 18 is fixedly connected to the inner wall of the sink groove 15.

[0027] By rotating the turntable 11, the slide plate 17 slides outwards under the action of centrifugal force, and the slide plate 17 drives the plug block 19 to retract and move inside the sink 15 through the driving groove 20 and the driving column 21, and the plug block 19 leaves the insertion hole 1203. At this time, the test tube sleeve 12 is released from the limit, which is convenient for the subsequent inclination angle to centrifuge the cell sample inside the centrifugal test tube. When the turntable 11 stops rotating, the slide plate 17 is reset under the action of the first spring 18. At this time, the plug block 19 is reinserted into the inside of the insertion hole 1203. The device is convenient for limiting the test tube sleeve 12 to prevent its shaking from affecting the subsequent packaging needle 5 to package the cell sample after centrifugation.

[0028] In this embodiment, an X-axis moving component 2 is slidably installed between the outer surfaces of the two opposite sides of the base 1, a Y-axis moving component 3 is arranged on the top of the X-axis moving component 2, a Z-axis moving component 4 is arranged on the outer surface of one side of the Y-axis moving component 3, and a dispensing needle 5 is arranged at the bottom of the Z-axis moving component 4.

[0029] The X-axis moving component 2, the Y-axis moving component 3 and the Z-axis moving component 4 drive the dispensing needle 5 to move to the top of the designated centrifuge test tube, extract the centrifuged cell fluid inside the centrifuge test tube in layers, and dispensing it for subsequent detection.

[0030] A method for using a cell centrifugal packaging device comprises the following steps: S1: When in use, the operator first inserts the centrifugal test tube containing the cell sample into the test tube sleeve 12, and then starts the driving motor 9, which drives the turntable 11 to rotate. The rotation of the turntable 11 causes the slide plate 17 to slide outwards under the action of centrifugal force, and the slide plate 17 drives the insert block 19 to retract into the sink 15 through the driving groove 20 and the driving column 21, and the insert block 19 leaves the insertion hole 1203. At this time, the test tube sleeve 12 is released from the limit; S2: The test tube sleeve 12 is tilted to drive the rubber friction wheel 28 to abut against the driving chamfer 1001 of the fixed plate 10. At this time, since the rotating plate 11 and the fixed plate 10 are in relative motion, the rubber friction wheel 28 rotates by friction with the driving chamfer 1001, and the rubber friction wheel 28 drives one of the rubber clamping wheels 24 to rotate, and the rubber clamping wheel 24 drives the centrifugal test tube to rotate. S3: The test tube sleeve 12 is driven to move by the turntable 11, and the centrifugal test tube is driven to move by the test tube sleeve 12. Due to the centrifugal force, the test tube sleeve 12 is tilted at an angle of 30° along the direction of the arc groove 31, and the sample inside the centrifugal test tube is subjected to the centrifugal force to separate cells or cell components of different densities from the liquid; S4: After the centrifugation is completed, the X-axis moving component 2, the Y-axis moving component 3 and the Z-axis moving component 4 drive the packaging needle 5 to move to the top of the designated centrifuge tube, extract the centrifuged cell fluid inside the centrifuge tube in layers, and package it for subsequent detection.

[0031] It should be noted that when the present invention is used, the operator first inserts the centrifugal test tube filled with the cell sample into the test tube sleeve 12, and then starts the drive motor 9, and drives the second gear 8 to rotate through the drive motor 9, and the second gear 8 drives the sleeve 6 to rotate through the first gear 7, and the sleeve 6 drives the turntable 11 to rotate, and the turntable 11 drives the test tube sleeve 12 to move, and the test tube sleeve 12 drives the centrifugal test tube to move. Due to the effect of centrifugal force, the test tube sleeve 12 will be tilted at an angle of 30° along the direction of the arc groove 31. At this time, the sample inside the centrifugal test tube is subjected to the centrifugal force to separate cells or cell components of different densities from the liquid, and the device is convenient for centrifugal separation of cell samples; The rotation of the turntable 11 causes the slide plate 17 to slide outwards under the action of the centrifugal force, and the slide plate 17 drives the plug block 19 to retract and move inside the sink 15 through the driving groove 20 and the driving column 21, and the plug block 19 leaves the insertion hole 1203. At this time, the test tube sleeve 12 is released from the limit, which is convenient for the subsequent tilt angle to centrifuge the cell sample inside the centrifugal test tube. When the turntable 11 stops rotating, the slide plate 17 is reset under the action of the first spring 18. At this time, the plug block 19 is reinserted into the inside of the insertion hole 1203. The device is convenient for limiting the test tube sleeve 12 to prevent its shaking from affecting the subsequent packaging needle 5 to package the cell sample after centrifugation. The tilting movement of the test tube sleeve 12 causes the top column 25 to slide on the inner wall of the arc groove 31. When the chamfer of the end face of the top column 25 slides relative to the arc bevel, it drives the two arc clamps 22 to move closer to the centrifuge test tube. The arc clamps 22 drive the internal multiple rubber clamping wheels 24 to press against the outer surface of the centrifuge test tube. The centrifuge test tube is limited by the device to avoid shaking during centrifugal movement. After the centrifugation is completed, the turntable 11 stops rotating. At this time, the test tube sleeve 12 returns to the vertical position. At the same time, the arc clamps 22 drive the internal multiple rubber clamping wheels 24 to leave the surface of the centrifuge test tube. When the centrifuge test tube is released from the limit by the device, there is no need to manually operate one by one. When batch cell centrifugation is performed, the workload of the operator is reduced and the efficiency of cell centrifugation is accelerated. The tilting movement of the test tube sleeve 12 drives the rubber friction wheel 28 to abut against the driving chamfer 1001 of the fixed disk 10. At this time, since the rotating disk 11 and the fixed disk 10 are in relative motion, the rubber friction wheel 28 rotates itself through the friction with the driving chamfer 1001, and the rubber friction wheel 28 drives one of the rubber clamping wheels 24 to rotate, and the rubber clamping wheel 24 drives the centrifugal test tube to rotate. The periodic centrifugal force changes generated by the rotation of the centrifugal test tube can slightly disturb the sedimentation, thereby preventing cells or particles from forming a hard knot at the bottom of the centrifugal test tube, which is convenient for subsequent resuspension; After the centrifugation is completed, the X-axis moving component 2, the Y-axis moving component 3 and the Z-axis moving component 4 drive the dispensing needle 5 to move to the top of the designated centrifuge tube, extract the centrifuged cell fluid inside the centrifuge tube in layers, and dispensing it for subsequent detection.

[0032] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A cell centrifugal packaging device, comprising a base (1), characterized in that: A sleeve (6) is rotatably mounted on the upper surface of the base (1); a turntable (11) is fixedly mounted on the circumferential outer surface of the sleeve (6) near the top; a plurality of openings (14) are equidistantly penetrated through the circumferential outer surface of the turntable (11); two rotating columns (1202) are symmetrically rotatably mounted on the inner walls of the plurality of openings (14) on opposite sides; a test tube sleeve (12) is fixedly mounted between adjacent ends of the two rotating columns (1202); a centrifugal test tube is inserted into the interior of the test tube sleeve (12); two support blocks (1201) are symmetrically fixedly mounted on the end surface of the top of the test tube sleeve (12); and the two support blocks (1201) are symmetrically mounted on the end surface of the top of the test tube sleeve (12). The inner wall of the support block (1201) is slidably inserted with a top column (25), and an adjacent end of the top column (25) penetrates the outer surface of the support block (1201) and is fixedly installed with an arc clamp (22), and the outer surface of the arc clamp (22) is provided with a mounting groove (23), and a plurality of rubber clamp wheels (24) are equidistantly rotatably installed between the inner walls of the mounting groove (23), and the plurality of rubber clamp wheels (24) are abutted against the circumferential outer surface of the centrifuge test tube, and the inner walls of the opening (14) on opposite sides are provided with arc grooves (31), and the top column (25) is inserted into the inside of the arc groove (31) and slidably installed with the inner wall thereof.

2. A cell centrifugal packaging device according to claim 1, characterized in that: A retaining ring (26) is fixedly mounted on the outer surface of the top column (25) near the circular arc groove (31); a second spring (27) is sleeved on the circumferential outer surface of the top column (25); the second spring (27) is arranged between the support block (1201) and the retaining ring (26); an inner wall of the circular arc groove (31) near the support block (1201) is provided with an arc bevel; an end surface of the top column (25) is provided with a chamfer matching the arc bevel; the chamfer of the end surface of the top column (25) is offset against the arc bevel; and the arc groove (31) and the rotation center of the rotating column (1202) are arranged at the same center.

3. A cell centrifugal packaging device according to claim 2, characterized in that: A fixing column (1002) is fixedly mounted on the upper surface of the base (1), the fixing column (1002) being arranged inside the sleeve (6), the top end of the fixing column (1002) passing through the upper surface of the turntable (11) and fixedly mounted with a fixing disk (10), the upper surface of the turntable (11) being provided with a circular groove (13), the fixing disk (10) being arranged inside the circular groove (13), the circumferential outer surface of the fixing disk (10) close to the top end being provided with a driving chamfer (1001), the top end of the rubber clamp wheel (24) close to the fixing disk (10) passing through the upper surface of the arc clamp (22) and fixedly mounted with a fourth gear (30).

4. A cell centrifugal packaging device according to claim 3, characterized in that: A third gear (29) is rotatably mounted on the circumferential outer surface of the arc clamp (22), the third gear (29) meshing with a fourth gear (30), a rubber friction wheel (28) is fixedly mounted on the upper surface at a middle position of the third gear (29), the circumferential outer surface of the rubber friction wheel (28) being spherical, the circumferential outer surface of the rubber friction wheel (28) abutting against the outer surface of the driving chamfer (1001).

5. A cell centrifugal packaging device according to claim 1, characterized in that: A first gear (7) is fixedly mounted on the circumferential outer surface of the sleeve (6) near the bottom end, a second gear (8) is rotatably mounted on the upper surface of the base (1), the second gear (8) meshing with the first gear (7), a drive motor (9) is fixedly mounted on the lower surface of the base (1), and an output end of the drive motor (9) passes through the upper surface of the base (1) and is fixedly mounted at the rotation center of the second gear (8).

6. A cell centrifugal packaging device according to claim 1, characterized in that: A plurality of troughs (15) are equidistantly provided in the circumferential direction of the lower surface of the turntable (11); a plurality of inner walls of the troughs (15) are slidably provided with slide plates (17); a dovetail slide groove (16) is provided on the top wall of the trough (15); a dovetail slide rail matching the dovetail slide groove (16) is provided on the upper surface of the slide plate (17); the dovetail slide rail is slidably provided on the inner wall of the dovetail slide groove (16); an arcuate slide groove is penetrated through the inner walls of the opposite ends of the trough (15); an insert block (19) is slidably provided on the inner wall of the arcuate slide groove.

7. A cell centrifugal packaging device according to claim 6, characterized in that: The test tube sleeve (12) has two symmetrically formed insertion holes (1203) on its circumferential outer surface. One end of the insertion block (19) is inserted into the insertion hole (1203). A driving column (21) is fixedly mounted on the upper surface of the other end of the insertion block (19). Two driving grooves (20) are symmetrically formed through the upper surface of the slide plate (17). The driving column (21) is slidably mounted on the inner wall of the driving groove (20). A first spring (18) is fixedly mounted on the outer surface of the slide plate (17) away from the sleeve (6). The other end of the first spring (18) is fixedly connected to the inner wall of the sink groove (15).

8. A cell centrifugal packaging device according to claim 1, characterized in that: An X-direction moving component (2) is slidably mounted between the outer surfaces of two opposite sides of the base (1); a Y-direction moving component (3) is arranged on the top of the X-direction moving component (2); a Z-direction moving component (4) is arranged on the outer surface of one side of the Y-direction moving component (3); and a dispensing needle (5) is arranged at the bottom of the Z-direction moving component (4).

9. A method for using a cell centrifugal packaging device, characterized in that: A cell centrifugal packaging device according to any one of claims 1 to 8 is used, comprising the following steps: S1: When in use, the operator first inserts the centrifugal test tube containing the cell sample into the test tube sleeve (12), and then starts the driving motor (9), and drives the turntable (11) to rotate through the driving motor (9). The rotation of the turntable (11) causes the slide plate (17) to slide outwards under the action of centrifugal force, and the slide plate (17) drives the insert block (19) to retract into the interior of the sink (15) through the driving groove (20) and the driving column (21), and the insert block (19) leaves the insertion hole (1203). At this time, the test tube sleeve (12) is released from the limit; S2: The test tube sleeve (12) is tilted to drive the rubber friction wheel (28) to abut against the driving chamfer (1001) of the fixed disk (10). At this time, since the rotating disk (11) and the fixed disk (10) are in relative motion, the rubber friction wheel (28) is rotated by friction with the driving chamfer (1001), and the rubber friction wheel (28) drives one of the rubber clamping wheels (24) to rotate, and the rubber clamping wheel (24) drives the centrifugal test tube to rotate. S3: The test tube sleeve (12) is driven to move by the turntable (11), and the centrifugal test tube is driven to move by the test tube sleeve (12). Due to the effect of centrifugal force, the test tube sleeve (12) is tilted at an angle of 30° along the direction of the arc groove (31), and the sample inside the centrifugal test tube is subjected to the centrifugal force to separate cells or cell components of different densities from the liquid; S4: After the centrifugation is completed, the X-axis moving component (2), the Y-axis moving component (3) and the Z-axis moving component (4) drive the dispensing needle (5) to move to the top of the designated centrifuge tube, extract the centrifuged cell fluid inside the centrifuge tube in layers, and dispense it for subsequent testing.

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