Chiral inorganic nano antibacterial film as well as preparation method and application thereof

Abstract

The invention provides a chiral inorganic nano antibacterial film as well as a preparation method and application thereof. The chiral inorganic nano antibacterial film comprises a chiral inorganic nano material and a biodegradable material, preparing a biodegradable material into a corresponding solution, adding the chiral inorganic nano-material solution under the condition of heating and stirring, and uniformly mixing to obtain a film forming solution; and preparing the antibacterial film by adopting a solution casting method. The chiral inorganic nano antibacterial film not only has excellent photo-thermal conversion performance and thermal stability, but also can efficiently kill bacteria under illumination, and is still very strong in antibacterial ability under dark conditions. The device can be applied to an instrument in deep sea operation, and can reduce the influence of microorganisms in the sea on the service life of the instrument and reduce loss.

Description

Technical Field

[0001] The invention relates to the field of material chemistry, and in particular to a chiral inorganic nano antibacterial film and a preparation method and application thereof. Background Art

[0002] Seawater breeds a large number of microorganisms, and precision instruments used for deep-sea operations need to be immersed in seawater for a long time. In addition to the impact of seawater itself on the life of the instrument, the microorganisms in the seawater will also adhere to the surface of the instrument, further accelerating the damage of the instrument. In response to the impact of seawater, some waterproof materials are usually used on the surface of the instrument to reduce the close contact between water and the instrument. Similarly, in order to reduce the attachment of microorganisms on the surface of the instrument or remove microorganisms on the instrument, antibacterial materials need to be applied. However, when operating in the deep sea, due to the deep dive, sunlight cannot directly shine in, and the environment is in a completely dark state. At this time, general photothermal antibacterial materials cannot play their full role. Therefore, it is necessary to find a material that can also perform high-efficiency antibacterial effects under dark conditions.

[0003] Chirality is an intrinsic property of nature, ranging from amino acids, polysaccharides and nucleic acid molecules that make up organisms to the universe. Chiral substances have special enantioselective biological activity. When the chiral specificity of the prepared nanomaterials is opposite to that of bacteria and other microorganisms, it is not easy to attach to the surface of the antibacterial film due to the selectivity of the organisms. Therefore, chiral inorganic nanomaterials are a good antibacterial material with excellent photothermal conversion performance and thermal stability, and can effectively kill bacteria under light. Summary of the invention

[0004] The present invention combines chiral inorganic nanomaterials with biodegradable membrane materials to prepare an antibacterial membrane, which has strong antibacterial ability under dark conditions, solving the antibacterial problem on the surface of deep-sea operating instruments under dark conditions. The purpose of the present invention is to provide a chiral inorganic nano antibacterial membrane.

[0005] The technical solution of the present invention is achieved in this way:

[0006] The present invention provides a chiral inorganic nano antibacterial film, characterized in that the chiral inorganic nano antibacterial film comprises a chiral inorganic nano material and a biodegradable material.

[0007] The chiral inorganic nanomaterials include but are not limited to chiral gold, chiral cobalt, chiral silver, and chiral zinc oxide; the morphology of the chiral inorganic nanomaterials includes but is not limited to sphere, cube, rod, and sheet.

[0008] Biodegradable materials include, but are not limited to, polyvinyl alcohol, polylactic acid, starch-based, and cellulose-based materials.

[0009] Furthermore, the mass ratio of the chiral inorganic nanomaterial to the biodegradable material is 1:100 to 30000.

[0010] Furthermore, the thickness of the antibacterial film is 40 to 120 μm.

[0011] Another object of the present invention is to provide a method for preparing the above-mentioned chiral inorganic nano antibacterial film: preparing a biodegradable material into a corresponding solution, adding a chiral inorganic nano material solution under heating and stirring, mixing evenly, and obtaining a film-forming solution; and preparing the antibacterial film by a solution casting method.

[0012] The third object of the present invention is the application of the chiral inorganic nano antibacterial film in inhibiting or fighting bacteria under dark conditions.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The chiral inorganic nano antibacterial film provided by the present invention not only has excellent light-to-heat conversion performance and thermal stability, can efficiently kill bacteria under light, but also has strong antibacterial ability under dark conditions. Therefore, the chiral inorganic nano antibacterial film is applied to instruments in deep-sea operations to reduce the impact of microorganisms in the ocean on the service life of the instrument and reduce losses. In addition, the nano antibacterial film also has biodegradable properties, and its preparation method is simple. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a graph showing the antibacterial performance of Example 1 against Escherichia coli and Staphylococcus aureus under dark conditions.

[0016] Figure 2 This is a graph showing the antibacterial performance of Example 2 against Escherichia coli and Staphylococcus aureus under dark conditions.

[0017] Figure 3 This is a graph showing the antibacterial performance of Example 3 against Escherichia coli and Staphylococcus aureus under dark conditions.

[0018] Figure 4 This is a graph showing the antibacterial performance of Example 4 against Escherichia coli and Staphylococcus aureus under dark conditions.

[0019] Figure 5 This is a graph showing the antibacterial performance of Example 5 against Escherichia coli and Staphylococcus aureus under dark conditions.

[0020] Figure 6 This is a graph showing the antibacterial performance of Example 6 against Escherichia coli and Staphylococcus aureus under dark conditions. DETAILED DESCRIPTION

[0021] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention. Those skilled in the art can make modifications or equivalent substitutions based on the understanding of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and all should be included in the protection scope of the present invention.

[0022] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0023] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0024] Example 1 Preparation of chiral gold nano-antibacterial film

[0025] Dissolve 1g of polyvinyl alcohol (PVA) in 10mL of ultrapure water, heat and stir at 97℃ and 300rpm for 3h. Add 1mL37.5μg / mL of left-handed gold nanomaterial to the above PVA solution and stir at 200rpm for 30min. After mixing evenly, pour into a 90mm Petri dish and dry in a 30℃ oven for 18h to obtain a left-handed gold nanoantibacterial film (PVA / L-AuNPS). Add 1mL37.5μg / mL of right-handed gold nanomaterial in the same way to prepare a right-handed gold nanoantibacterial film (PVA / D-AuNPS).

[0026] Example 2 Preparation of chiral gold nano-antibacterial film

[0027] Dissolve 1g chitosan (CS) in 10mL 1% acetic acid aqueous solution, then add glycerol and stir at room temperature for 1h. Add 2mL 37.5μg / mL left-handed gold nanomaterials and mix and stir for 1h. Finally, remove the bubbles in the solution by ultrasound for 30min. Pour the solution into a petri dish with a diameter of 90mm and dry it at 45℃ for 24h to obtain a left-handed gold nanoantibacterial film (CS / L-Au NPS). Add 2mL 37.5μg / mL right-handed gold nanomaterials in the same way to prepare a right-handed gold nanoantibacterial film (CS / D-Au NPS).

[0028] Example 3 Preparation of chiral gold nanoparticle antibacterial film

[0029] Dissolve 1g of polylactic acid (PLA) in 10mL of chloroform and stir continuously at 25°C for 6h. Add 3mL of 37.5μg / mL left-handed gold nanomaterials, mix and stir for 5h, and use a rod-type automatic film coating device to control the film thickness to 60-70μm. Finally, dry the film in an oven at 60°C for 24h to obtain a left-handed gold nanoantibacterial film (PLA / L-Au NPS). In the same way, add 3mL of 37.5μg / mL right-handed gold nanomaterials to prepare a right-handed gold nanoantibacterial film (PLA / D-Au NPS).

[0030] Example 4 Preparation of chiral cobalt nano antibacterial film

[0031] 1g of polyvinyl alcohol (PVA) was dissolved in 10mL of ultrapure water, and heated and stirred at 97°C and 300rpm for 3h. 3mg of left-handed cobalt nanomaterial was added to 1mL of ultrapure water, and after ultrasonic homogenization, it was blended with the above-mentioned PVA solution and stirred at 200rpm for 30min. After mixing evenly, it was poured into a 90mm Petri dish and dried in a 30°C oven for 18h to obtain a left-handed cobalt nano antibacterial film (PVA / L-Co NPS). 3mg of right-handed cobalt nanomaterial was added in the same way to prepare a right-handed gold nano antibacterial film (PVA / D-Co NPS).

[0032] Example 5 Preparation of chiral cobalt nano antibacterial film

[0033] 1g chitosan (CS) was dissolved in 10mL 1% acetic acid aqueous solution, and then glycerol was added and stirred at room temperature for 1h. Then 4mg of left-handed cobalt nanomaterials were added to the above CS solution for blending and stirred for 1h. Finally, the bubbles in the solution were removed by ultrasound for 30min. The solution was poured into a culture dish with a diameter of 90mm and dried at 45℃ for 24h to obtain a left-handed cobalt nano antibacterial film (CS / L-Co NPS). 4mg of right-handed cobalt nanomaterials were added in the same way to prepare a right-handed gold nano antibacterial film (CS / D-Co NPS).

[0034] Example 6 Preparation of chiral cobalt nano antibacterial film

[0035] Dissolve 1g of polylactic acid (PLA) in 10mL of chloroform and stir continuously at 25°C for 6h. Add 5mg of left-handed cobalt nanomaterials and mix and stir for 5h, and use a rod-type automatic film coating device to control the film thickness to 60-70μm. Finally, dry the film in an oven at 60°C for 24h to obtain a left-handed cobalt nano antibacterial film (PLA / L-Co NPS). In the same way, add 5mg of right-handed cobalt nanomaterials to prepare a right-handed gold nano antibacterial film (PLA / D-Co NPS).

[0036] Antibacterial Effect Example

[0037] 1. Antibacterial effect of chiral gold nano-antibacterial film

[0038] The chiral gold nanoantibacterial film prepared in Example 1-3 was cut into 2 cm × 2 cm size and placed in a 2 mL centrifuge tube. 100 μL of bacterial solution (1 × 10 7 CFU·mL -1 ) and 900 μL PBS solution were completely in contact with the antibacterial film. The bacterial solution without antibacterial film was used as the control group. All groups were wrapped in tin foil to avoid light and then incubated at 37°C for 12 hours. After the incubation, the bacterial suspensions after different treatments were diluted to 10 4 CFU·mL -1 Finally, 100 μL of the diluted bacterial suspension was placed on a gel-like LB agar plate, spread evenly, and incubated in a 37°C incubator for 24 h. The antibacterial activity of different samples was studied using the standard colony counting method, and the above steps were repeated three times.

[0039] As attached Figure 1 As shown in the figure, compared with the pure PVA film, the chiral gold nano-antibacterial film prepared in Example 1 has a certain reduction in the number of Escherichia coli and Staphylococcus aureus colonies; Figure 2 As shown, the chiral gold nano antibacterial film prepared in Example 2 has extremely strong antibacterial activity against Escherichia coli and Staphylococcus aureus. In the two groups of CS / D-AuNPs film against Escherichia coli and CS / L-AuNPs film against Staphylococcus aureus, there is basically no bacterial growth in the culture dish; Figure 3 As shown, the chiral gold nanoantibacterial film prepared in Example 3 also has extremely strong antibacterial activity against Escherichia coli and Staphylococcus aureus, among which the PLA / D-AuNPs film has a good killing effect on both Escherichia coli and Staphylococcus aureus, and there is basically no bacterial growth in the culture dish.

[0040] 2. Antibacterial effect of chiral cobalt nano-antibacterial film

[0041] The chiral cobalt nano antibacterial film prepared in Example 4-6 was cut into 2 cm × 2 cm size and placed in a 2 mL centrifuge tube. 100 μL of bacterial solution (1 × 10 7 CFU·mL -1 ) and 900 μL PBS solution were completely in contact with the antibacterial film. The bacterial solution without antibacterial film was used as the control group. All groups were wrapped in tin foil to avoid light and then incubated at 37°C for 12 hours. After the incubation, the bacterial suspensions after different treatments were diluted to 10 4 CFU·mL -1Finally, 100 μL of the diluted bacterial suspension was placed on a gel-like LB agar plate, spread evenly, and incubated in a 37°C incubator for 24 h. The antibacterial activity of different samples was studied using the standard colony counting method, and the above steps were repeated three times.

[0042] As attached Figure 4 As shown in the figure, compared with the pure PVA film, the chiral cobalt nano antibacterial film prepared in Example 4 has significantly reduced colony counts of Escherichia coli and Staphylococcus aureus; Figure 5 As shown, the chiral cobalt nano antibacterial film prepared in Example 5 has extremely strong antibacterial activity against Escherichia coli and Staphylococcus aureus, especially against Staphylococcus aureus. No colony grows in the culture dish in either the CS / L-Co SS film group or the CS / D-Co SS film group. Figure 6 As shown, the chiral cobalt nano-antibacterial film prepared in Example 6 has a highly effective bactericidal ability against Escherichia coli and Staphylococcus aureus. Both the PLA / L-CoSS film and the PLA / D-Co SS film have a stronger killing effect on Staphylococcus aureus, resulting in the phenomenon of no colony growth in the culture dish.

[0043] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A chiral inorganic nano antibacterial film, characterized in that: The chiral inorganic nano antibacterial film comprises chiral inorganic nano materials and biodegradable materials.

2. The chiral inorganic nano antibacterial film according to claim 1, characterized in that: Chiral inorganic nanomaterials include, but are not limited to, chiral gold, chiral cobalt, chiral silver, and chiral zinc oxide.

3. The chiral inorganic nano antibacterial film according to claim 1, characterized in that: The morphology of chiral inorganic nanomaterials includes but is not limited to sphere, cube, rod, and sheet.

4. The chiral inorganic nano antibacterial film according to claim 1, characterized in that: Biodegradable materials include, but are not limited to, polyvinyl alcohol, polylactic acid, starch-based, and cellulose-based materials.

5. The chiral inorganic nano antibacterial film according to claim 1, characterized in that: The mass ratio of the chiral inorganic nanomaterial to the biodegradable material is 1:100 to 30000.

6. The chiral inorganic nano antibacterial film according to claim 1, characterized in that: The thickness of the antibacterial film is 40 to 120 μm.

7. A method for preparing a chiral inorganic nano antibacterial film according to any one of claims 1 to 6, characterized in that: The preparation method is as follows: the biodegradable material is configured into a corresponding solution, and the chiral inorganic nano material solution is added under heating and stirring, and mixed evenly to obtain a film-forming solution; and the antibacterial film is prepared by a solution casting method.

8. Use of the chiral inorganic nano antibacterial film according to any one of claims 1 to 6 for antibacterial or antimicrobial purposes under dark conditions.

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