Cell recovery device

Abstract

The invention provides a cell resuscitation device which comprises a box body, and the box body is of a circular-truncated-cone-shaped barrel body structure with an open upper end face; the rotating mechanism comprises a clamping part capable of clamping a test tube and a driving part, and the clamping part is arranged in the box body and is connected with the driving part, so that the clamping part is driven by the driving part to do circular motion around the central axis; the heating mechanism is arranged in the center of the box body and arranged on the driving part in a sleeving manner so as to heat the test tubes which do circular motion; the console is electrically connected with the rotating mechanism and the heating mechanism to control the rotating mechanism and the heating mechanism to work; through the structural design, the rotating mechanism is utilized to enable the clamping part to clamp a test tube to rotate, the test tube is uniformly heated in the moving process to avoid local overheating, and the clamping mechanism part replaces a human hand to directly touch the test tube to reduce the risk of pollution, so that the problem that the current cell resuscitation quality does not reach the standard is solved.

Description

Technical Field

[0001] The invention relates to the technical field of cell cryopreservation, and in particular to a cell resuscitation device. Background Art

[0002] In the biomedical field, cell freezing and thawing are core technologies to ensure cell activity, but existing thawing devices generally use static heat transfer and passive diffusion modes, which cause irreversible damage to cells during phase change. Taking the water bath heating method as an example, it relies on an external medium, such as a 37°C water bath, to conduct unidirectional heat conduction to the frozen test tube. Due to the difference in thermal resistance inside and outside the test tube and the axial temperature gradient, the measured temperature difference between the tube mouth and the bottom of the tube is 15-28°C. The lipid layer of the cell membrane undergoes non-uniform phase change due to local overheating or overcooling, triggering secondary growth of ice crystals, which directly leads to cell membrane perforation and intracellular protein denaturation, and a 22%-35% decrease in cell survival rate.

[0003] Moreover, since the mixing method relies on manual shaking or pipetting, not only does the mixing efficiency fluctuate greatly, but also introduces the risk of microbial contamination due to differences in operator techniques. The above problems seriously restrict the consistency and large-scale production of cell therapy products. Therefore, it is urgent to develop a cell resuscitation device with sterile self-mixing function to meet the quality control standards for large-scale production of cell therapy products. Summary of the invention

[0004] The main purpose of the present invention is to provide a cell resuscitation device to at least solve the problem of substandard cell resuscitation quality in the prior art.

[0005] In order to achieve the above-mentioned purpose, the present invention provides a cell recovery device comprising: a box body, which is a truncated cone-shaped barrel structure with an open upper end surface; a rotating mechanism, which comprises a clamping component and a driving component that can clamp a test tube, the driving component is arranged inside the box body along the central axis of the box body, the clamping component is arranged inside the box body and is connected to the driving component so that the clamping component performs a circular motion around the central axis under the drive of the driving component; a heating mechanism, which is arranged inside the center of the box body and the heating mechanism is sleeved on the driving component to heat the test tube performing a circular motion; wherein the clamping component comprises a first clamping component and a second clamping component, and the test tube can be rotatably clamped The driving component comprises: a first connecting arm and a second connecting arm, wherein the first end of the first connecting arm is connected to the first clamping component, and the first end of the second connecting arm is connected to the second clamping component; the first connecting arm is hinged to the first clamping component; the second connecting arm is a telescopic arm, and the extending arm of the second connecting arm is hinged to the second clamping component, and a cylinder is provided on the fixed tube of the second connecting arm, and the output end of the cylinder is connected to the extending arm to extend or retract the extending arm; the first connecting arm and the second connecting arm cooperate with each other to tilt the clamping component.

[0006] Furthermore, the first clamping component and the second clamping component are two circular plate structures with circular through holes in the center; an annular rubber ring is provided at the through hole to clamp the test tube; the clamping component also includes: a swivel, the swivel is arranged at the through holes of the first clamping component and the second clamping component, the annular rubber ring is arranged at the inner circumferential wall of the swivel, an annular limiting plate is arranged on the outer circumferential wall of the swivel, the first clamping component and the second clamping component are both provided with limiting grooves matching the limiting plates so that the swivel can be rotatably arranged on the first clamping component and the second clamping component; a second motor, the second motor is arranged at the bottom of the second clamping component, and the second motor is connected to the swivel of the second clamping component to enable it to rotate.

[0007] Furthermore, the driving component includes: a first motor, which is arranged at the bottom of the box; a rotating shaft, which is arranged inside the box along the central axis of the box, and one end of the rotating shaft passes through the bottom of the box and is connected to the first motor; the second end of the first connecting arm is connected to the rotating shaft; and the second end of the second connecting arm is connected to the rotating shaft.

[0008] Furthermore, there are multiple clamping mechanisms, and the multiple clamping mechanisms are evenly spaced along the circumferential direction and arranged in the box body.

[0009] Furthermore, the heating mechanism is sleeved on the rotating shaft between the first clamping part and the second clamping part, and the heating mechanism also includes: an outer shell, the outer shell is a truncated cone-shaped shell structure, a plurality of air outlets are evenly spaced circumferentially on the side wall of the outer shell, and a plurality of air inlets are opened at the bottom center of the outer shell; a hair dryer, a plurality of hair dryers are evenly spaced inside the outer shell; a heating wire, the heating wire is fixedly arranged at the blowing outlets of the plurality of hair dryers.

[0010] Furthermore, the driving component also includes a limiting ring, which is arranged between the multiple first connecting arms and the outer shell and fixedly connected to the multiple first connecting arms; a matching sliding groove is arranged on the top surface of the outer shell to keep the multiple first connecting arms stable during movement.

[0011] Furthermore, a corresponding heat preservation cover is provided at the opening of the box to keep the interior of the box warm; the cell resuscitation device also includes: a control console, which is arranged on the heat preservation cover; the control console is electrically connected to the rotating mechanism and the heating mechanism to control the operation of the rotating mechanism and the heating mechanism.

[0012] Furthermore, a first temperature detector is provided on the inner wall of the box body, a second temperature detector is provided on the clamping mechanism, and the second temperature detector is provided with a probe to measure the temperature of the test tube surface; the console is electrically connected to the first temperature detector and the second temperature detector to monitor the temperature.

[0013] Furthermore, support feet are provided at the bottom of the box, and ventilation holes are provided on the bottom surface of the box.

[0014] The cell resuscitation device using the technical solution of the present invention comprises: a box body, which is a truncated cone-shaped barrel structure with an open upper end surface; a corresponding heat preservation cover is arranged at the open end of the box body to keep the inside of the box body warm; a rotating mechanism, which comprises a clamping component and a driving component that can clamp a test tube, the driving component is arranged inside the box body along the central axis of the box body, the clamping component is arranged inside the box body and connected to the driving component so that the clamping component performs a circular motion around the central axis under the drive of the driving component; a heating mechanism, which is arranged inside the center of the box body and the heating mechanism is sleeved on the driving component to heat the test tube performing a circular motion; a control console, which is arranged on the heat preservation cover; the control console It is electrically connected to the rotating mechanism and the heating mechanism to control the operation of the rotating mechanism and the heating mechanism. Through such a structural design, the present invention achieves a dual improvement in temperature uniformity and dynamic mixing of samples during cell resuscitation. The rotating mechanism is used to make the clamping component clamp the test tube for rotation, and the test tube is evenly heated during the movement to avoid local overheating. At the same time, the setting of the thermal insulation cover ensures that the temperature inside the box is constant, which is convenient for staff to control. The clamping mechanism components replace human hands to directly touch the test tube, reducing the risk of contamination. On the whole, the present invention provides an efficient and sterile cell resuscitation device, which effectively solves the problem of substandard cell resuscitation quality in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The drawings constituting a part of the present application are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings: Figure 1 is a schematic diagram of the external structure of a cell resuscitation device that can be selected according to an embodiment of the present invention; Figure 2 is a schematic diagram of the bottom structure of a cell resuscitation device that can be selected according to an embodiment of the present invention; Figure 3 is a schematic diagram of the internal structure of an optional cell resuscitation device according to an embodiment of the present invention; Figure 4 is a schematic diagram of a rotating mechanism structure of a cell resuscitation device that can be selected according to an embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of a clamping component of a cell resuscitation device that can be selected according to an embodiment of the present invention; Figure 6 is an exploded view of a clamping component of a cell resuscitation device that can be selected according to an embodiment of the present invention; Figure 7 Schematic diagram of the internal structure of a heating mechanism of a cell resuscitation device according to an embodiment of the present invention.

[0016] The above drawings include the following reference numerals: 10. Box body; 11. Insulation cover; 12. First temperature detector; 13. Second temperature detector; 131. Probe; 20. Rotating mechanism; 14. Support foot; 15. Ventilation hole; 30. Clamping part; 40. Driving part; 50. Heating mechanism; 60. Control console; 31. First clamping part; 32. Second clamping part; 33. Annular rubber ring; 34. Rotating ring; 341. Limiting plate; 35. Limiting groove; 36. Second motor; 41. First connecting arm; 42. Second connecting arm; 421. Extending arm; 422. Fixed pipe; 423. Cylinder; 43. Rotating shaft; 44. First motor; 45. Limiting ring; 51. Outer shell; 52. Air outlet; 53. Air inlet; 54. Hair dryer; 541. Air outlet; 55. Heating wire; 56. Slide groove. DETAILED DESCRIPTION

[0017] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0018] According to one embodiment of the present invention, a cell resuscitation device is disclosed, such as Figures 1 to 7As shown, it includes a box body 10, which is a truncated cone-shaped barrel structure with an open upper end surface; a corresponding heat-insulating cover 11 is provided at the open end of the box body 10 to keep the interior of the box body 10 warm; a rotating mechanism 20, which includes a clamping component 30 and a driving component 40 that can clamp a test tube, and the driving component 40 is arranged inside the box body 10 along the central axis of the box body 10, and the clamping component 30 is arranged inside the box body 10 and connected to the driving component 40 so that the clamping component 30 performs a circular motion around the central axis under the drive of the driving component 40; a heating mechanism 50, which is arranged inside the center of the box body 10 and the heating mechanism 50 is sleeved on the driving component 40 to heat the test tube that performs a circular motion; a control console 60, which is arranged inside the heat-insulating cover 11 and is sleeved on the driving component 40 to heat the test tube that performs a circular motion; and a control console 60. The control console 60 is electrically connected to the rotating mechanism 20 and the heating mechanism 50 to control the operation of the rotating mechanism 20 and the heating mechanism 50. Through such a structural design, the present invention achieves a dual improvement in temperature uniformity and dynamic mixing of samples during cell recovery. The rotating mechanism 20 is used to rotate the clamping component 30 while clamping the test tube, and the test tube is evenly heated during the movement to avoid local overheating. At the same time, the setting of the thermal insulation cover 11 ensures that the internal temperature of the box body 10 is constant, which is convenient for the staff to control. The clamping component 30 replaces the human hand to directly touch the test tube, reducing the risk of contamination. On the whole, the present invention provides an efficient and sterile cell recovery device, which effectively solves the problem of substandard cell recovery quality in the prior art.

[0019] Furthermore, if Figures 2 to 6As shown, the clamping component 30 includes a first clamping component 31 and a second clamping component 32. The first clamping component 31 and the second clamping component 32 are two circular plate structures with circular through holes in the center, and the coaxiality error of the through holes is less than 0.05 mm. An annular rubber ring 33 is provided at the through hole to clamp the test tube. The annular rubber ring 33 is made of medical silicone and has an anti-slip pattern on the inner wall, which can be adapted to various test tubes with a diameter of 12-50 mm. The clamping component 30 also includes a swivel 34, which is arranged at the through holes of the first clamping component 31 and the second clamping component 32. The annular rubber ring 33 is provided at the through hole to clamp the test tube. The annular rubber ring 33 is made of medical silicone and has an anti-slip pattern on the inner wall, which can be adapted to various test tubes with a diameter of 12-50 mm. The ring 33 is arranged at the inner peripheral wall of the rotating ring 34, and an annular limiting plate 341 is arranged on the outer peripheral wall of the rotating ring 34. The first clamping part 31 and the second clamping part 32 are both provided with limiting grooves 35 matching the limiting plates 341 so that the rotating ring 34 can be rotatably arranged on the first clamping part 31 and the second clamping part 32. The limiting grooves 35 can be embedded with ball bearings so that the rotation resistance coefficient of the rotating ring 34 is lower than 0.01; the second motor 36 is arranged at the bottom of the second clamping part 32, and the second motor 36 is connected to the rotating ring 34 of the second clamping part 32 to rotate it. Among them, the outer edge of the rotating ring 34 of the second clamping part 32 is gear-shaped, and the gear on the output shaft of the second motor 36 is meshed with the gear on the outer edge of the rotating ring 34 of the second clamping part 32. When the second motor 36 is working, the gear on the output shaft of the second motor 36 rotates to drive the rotating ring 34 of the second clamping part 32 to rotate. The speed of the second motor 36 can be set to 5-30rpm through the control console 60. The shear force generated when driving the test tube to rotate can directionally break up the cell clusters. The annular rubber ring 33 can fit tightly against the outer wall of the test tube, so that the rotating ring 34 can drive the test tube to rotate together during the rotation process to avoid idling.

[0020] Furthermore, if Figures 2 to 6As shown, the driving component 40 includes: a first motor 44, which is arranged at the bottom of the box body 10. A servo motor and a reducer can be used, and the speed control accuracy is ±1rpm to avoid excessive speed damaging the cells; a rotating shaft 43, which is arranged inside the box body 10 along the central axis of the box body 10, and one end of the rotating shaft 43 passing through the bottom of the box body 10 is connected to the first motor 44, and the surface of the rotating shaft 43 can be hard chrome plated to reduce friction loss; a first connecting arm 41, one end of the first connecting arm 41 is connected to the first clamping component 31, and the other end of the first connecting arm 41 is connected to the rotating shaft 43, and its hinge structure adopts a stainless steel universal joint, allowing the first clamping component 31 to be fine-tuned within the horizontal plane by ±15°; a second connecting arm 42, one end of the second connecting arm 42 is connected to the second clamping component 32, and the other end of the second connecting arm 42 is connected to the rotating shaft 43; the second connecting arm 42 is a telescopic arm, and the extending arm 421 of the second connecting arm 42 The second connecting arm 42 is hinged with the second clamping part 32, and a cylinder 423 is provided on the fixed tube 422 of the second connecting arm 42. The output end of the cylinder 423 is connected to the extending arm 421 to extend or retract the extending arm 421. The cylinder 423 adopts a closed-loop control type pneumatic cylinder with a displacement accuracy of ±0.1mm. When the cylinder 423 pushes the extending arm 421 to extend, the hinged part at the connection between the extending arm 421 and the second clamping part 32 rotates. Since the first connecting arm 41 cannot be extended or retracted, the hinged part between the first connecting arm 41 and the first clamping part 31 also rotates accordingly under the action of the second clamping part 32, thereby causing the test tube to have an inclination angle. The travel range of the telescopic arm is 50-150mm. By adjusting the extension amount, the test tube can form an inclination angle of 5°-30°. The centrifugal force generated by the revolution in the inclined state is decomposed into axial and radial components. The axial component prevents cells from settling and adhering to the wall, and the radial component promotes uniform mixing of the culture medium, thereby making it more evenly heated and vibrated.

[0021] Furthermore, if Figures 2 to 6 As shown, there are multiple clamping components 30, and the multiple clamping components 30 are evenly spaced along the circumferential direction in the box body 10. The spacing angle between adjacent clamping components 30 is 90° or 60°. Four or six groups can be selected for installation according to the length of the test tube, which not only improves work efficiency, but also controls the variables of the test and avoids differences in resuscitation of test tubes in the same batch caused by human subjective factors.

[0022] Furthermore, if Figures 2 to 7As shown, the heating mechanism 50 is sleeved on the rotating shaft 43 between the first clamping part 31 and the second clamping part 32, and the heating mechanism 50 also includes: an outer shell 51, the outer shell 51 is a truncated cone-shaped shell structure, and its taper matches the box 10 to match the circular motion when the test tube is tilted; a plurality of air outlets 52 are evenly spaced circumferentially on the side wall of the outer shell 51, and the air outlets 52 can be set into a vertical long strip to match the test tube; a plurality of air inlets 53 are opened at the bottom center of the outer shell 51, and a HEPA filter can be installed at the air inlet 53 to ensure air cleanliness; a hair dryer 54, there are a plurality of hair dryers 54, and the plurality of hair dryers 54 are evenly spaced inside the outer shell 51, and a brushless DC fan can be used, and the wind speed can be adjusted in the range of 2-10m / s; a heating wire 55, the heating wire 55 is fixedly set at the blowing port 541 of the plurality of hair dryers 54, and a nickel-chromium alloy material can be used and wrapped with a ceramic insulating tube, and the power density reaches 5W / cm², and the air flow can be heated to a set temperature within 30 seconds.

[0023] Furthermore, if Figures 2 to 6 As shown, the driving component 40 also includes a limit ring 45, which is arranged between the multiple first connecting arms 41 and the outer shell 51 and fixedly connected to the multiple first connecting arms 41. The limit ring 45 can be embedded with a graphite lubricating layer to reduce friction heat; the top surface of the outer shell 51 is provided with a matching slide groove 56 to enable the multiple first connecting arms 41 to remain stable during movement. The slide groove 56 is made of hardened steel and has a surface roughness Ra≤0.8μm, ensuring that the rotating mechanism 20 is not stuck in a high temperature environment.

[0024] Furthermore, if Figures 2 to 6 As shown, a first temperature detector 12 is provided on the inner wall of the box body 10, and a platinum resistance sensor can be used to monitor the ambient temperature; a second temperature detector 13 is also provided on the clamping component 30, and the second temperature detector 13 is provided with a probe 131 to measure the temperature of the test tube surface, and the needle of the probe 131 adopts a conical probe to ensure close contact with the outer wall of the test tube; the control console 60 is electrically connected to the first temperature detector 12 and the second temperature detector 13 to monitor the temperature. When the temperature difference between the ambient temperature and the test tube surface exceeds 0.5°C, the heating power can be automatically adjusted to achieve precise control and avoid human errors.

[0025] Furthermore, if Figures 2 to 6 As shown, support feet 14 are provided at the bottom of the box 10, and ventilation holes 15 are provided on the bottom surface of the box 10 to facilitate the heating mechanism 50 to absorb air, while also preventing local heat accumulation of mechanical parts from affecting normal operation to a certain extent.

[0026] During specific use, the staff opens the heat preservation cover 11, inserts multiple frozen test tubes into the multiple clamping parts 30, and the multiple test tubes pass through the first clamping part 31. The bottom of the test tube is inserted into the second clamping part 32 to complete the fixation. The annular rubber ring 33 on the first clamping part 31 and the second clamping part 32 fits tightly with the outer wall of the test tube to provide sufficient friction, thereby holding the test tube tightly. Before inserting the test tube, the staff can also wipe the outer wall of the test tube to avoid water droplets on the outer wall of the frozen test tube at room temperature that may slip and affect the fixation; operate the control console 60, and control the cylinder 423 through the control console 60 to adjust the extension range of the extension arm 421. The extended extension arm 421 pushes the second clamping part 32 outward along a predetermined direction. Since the first connecting arm 41 cannot be extended, the clamping part 30 rotates at the hinge position between the first clamping part 31 and the first connecting arm 41. The second clamping component 32 and the second connecting arm 42 also rotate synchronously at the hinged position; after adjusting the appropriate angle, the second motor 36 is started by using the control console 60 to rotate the test tube driven by the rotating ring 34, and the appropriate frequency is further adjusted; finally, after checking that the inside of the box 10 is correct, the insulation cover 11 is covered, the appropriate heating power and heating time are selected, and then the device is started; the first motor 44 drives the clamping component 30 to carry the test tube to rotate around the heating mechanism 50, and the heating mechanism 50 begins to gradually heat to a predetermined temperature under the operation of the control console 60. The staff can use the first temperature detector 12 to observe the internal temperature of the box 10, and control the power of the heating mechanism 50 when the predetermined temperature is reached; at the same time, the second temperature detector 13 is observed, and after a certain period of time, the control console 60 is operated to stop the device, open the insulation cover 11 to take out the test tube, and complete cell recovery.

[0027] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell resuscitation device, characterized in that: include: A box body, wherein the box body is a truncated cone-shaped barrel structure with an open upper end surface; A rotating mechanism, the rotating mechanism comprising a clamping component capable of clamping a test tube and a driving component, the driving component being arranged inside the box along the central axis of the box, the clamping component being arranged inside the box and connected to the driving component so that the clamping component performs a circular motion around the central axis under the drive of the driving component; A heating mechanism, the heating mechanism is arranged inside the center of the box body and the heating mechanism is sleeved on the driving component to heat the test tube performing a circular motion; Wherein, the clamping component comprises a first clamping component and a second clamping component, and the test tube is rotatably clamped on the first clamping component and the second clamping component; The driving component comprises: a first connecting arm and a second connecting arm, wherein a first end of the first connecting arm is connected to the first clamping component, and a first end of the second connecting arm is connected to the second clamping component; The first connecting arm is hinged to the first clamping part; the second connecting arm is a telescopic arm, the extending arm of the second connecting arm is hinged to the second clamping part, a cylinder is provided on the fixed tube of the second connecting arm, the output end of the cylinder is connected to the extending arm to extend or retract the extending arm; the first connecting arm and the second connecting arm cooperate with each other to tilt the clamping part.

2. The cell resuscitation device according to claim 1, characterized in that: The first clamping component and the second clamping component are two circular plate structures with circular through holes in the center; an annular rubber ring is provided at the through hole to clamp the test tube; the clamping component also includes: A swivel, the swivel is arranged at the through holes of the first clamping component and the second clamping component, the annular rubber ring is arranged at the inner peripheral wall of the swivel, an annular limiting plate is arranged on the outer peripheral wall of the swivel, and the first clamping component and the second clamping component are both provided with limiting grooves matching the limiting plate so that the swivel can be rotatably arranged on the first clamping component and the second clamping component; A second motor is disposed at the bottom of the second clamping component, and the second motor is connected to the rotating ring of the second clamping component to rotate it.

3. The cell resuscitation device according to claim 2, characterized in that: The driving component comprises: A first motor, wherein the first motor is arranged at the bottom of the box; A rotating shaft, the rotating shaft is arranged inside the box along the central axis of the box, and one end of the rotating shaft passes through the bottom of the box and is connected to the first motor; The second end of the first connecting arm is connected to the rotating shaft; The second end of the second connecting arm is connected to the rotating shaft.

4. The cell resuscitation device according to claim 1, characterized in that: There are a plurality of clamping components, and the plurality of clamping components are evenly spaced and arranged in the box along the circumferential direction.

5. The cell resuscitation device according to claim 3, characterized in that: The heating mechanism is sleeved on the rotating shaft between the first clamping member and the second clamping member, and the heating mechanism further comprises: An outer shell, the outer shell is a truncated cone-shaped shell structure, a plurality of air outlets are evenly spaced circumferentially on the side wall of the outer shell, and a plurality of air inlets are opened at the center of the bottom of the outer shell; A hair dryer, wherein the hair dryers are multiple and the multiple hair dryers are evenly spaced and arranged inside the outer shell; A heating wire is fixedly arranged at the air outlets of the plurality of hair dryers.

6. The cell resuscitation device according to claim 5, characterized in that: The driving component also includes a limiting ring, which is arranged between the multiple first connecting arms and the outer shell and fixedly connected to the multiple first connecting arms; a matching sliding groove is arranged on the top surface of the outer shell to ensure that the multiple first connecting arms remain stable during movement.

7. The cell resuscitation device according to claim 1, characterized in that: The opening of the box is provided with a corresponding heat-insulating cover to keep the interior of the box warm; the cell resuscitation device also includes: A control console is arranged on the heat-insulating cover; the control console is electrically connected to the rotating mechanism and the heating mechanism to control the operation of the rotating mechanism and the heating mechanism.

8. The cell resuscitation device according to claim 7, characterized in that: A first temperature detector is disposed on the inner wall of the box body, a second temperature detector is also disposed on the clamping component, and the second temperature detector is provided with a probe to measure the temperature of the test tube surface; the console is electrically connected to the first temperature detector and the second temperature detector to monitor the temperature.

9. The cell resuscitation device according to claim 1, characterized in that: The bottom of the box is provided with supporting feet, and the bottom surface of the box is provided with ventilation holes.

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