Device and method for efficiently detecting content of bacteria in tubular column based on shale gas exploitation system

Abstract

The invention discloses an efficient detection device and method for the content of bacteria in a tubular column based on a shale gas exploitation system, and relates to the technical field of detection of the content of bacteria. The machine table is supported on the ground, the auxiliary detection assembly is arranged on the machine table and used for collecting sample water, automatically and independently separating bacteria in the sample water and capable of automatically shaking a reagent, and a liquid adding and shading assembly located on the right side of the auxiliary detection assembly is further arranged on the machine table; and the right end part of the rotating shaft is provided with a biological membrane mounting assembly positioned right below the straight cylinder. The device has the beneficial effects that the working intensity of workers is relieved, the detection efficiency of the content of bacteria in the tubular column is greatly improved, and the displacement of the variable pump can be changed in time.

Description

Technical Field

[0001] The present invention relates to the technical field of detecting bacterial content, and in particular to a highly efficient detection device and method for bacterial content in a pipe column based on a shale gas production system. Background Art

[0002] The structure of the shale gas extraction system used in a certain area is as follows: Figure 1 As shown, it is used to extract shale gas from underground. The shale gas extraction system includes a wellbore 1 fixed in the well, a gas production tree 2 fixed on the top surface of the wellbore 1, a pipe column 3 is arranged in the wellbore 1, the top end of the pipe column 3 is fixed on the gas production tree 2, a gas-water separator 5 is connected to the end valve 4 of the gas production tree 2, a gas transmission pipeline 6 and a drainage pipeline 7 are connected to the gas-water separator 5, a first stop valve 8 is connected to the drainage pipeline 7, a desander 9 is connected to the end of the first stop valve 8, a second stop valve 10 is connected to the end of the desander 9, a water outlet pipe 11 is connected to the other end of the second stop valve 10, and in the initial state, the first stop valve 8 and the second stop valve 10 are both in a closed state; The shale gas extraction system also includes a variable pump 12 and a bactericidal corrosion inhibitor storage tank 13 arranged on the ground. The suction port of the variable pump 12 is connected to the bactericidal corrosion inhibitor storage tank 13. The discharge port of the variable pump 12 is connected to a curved pipe 14. The other end of the curved pipe 14 passes through the side wall of the wellbore 1 and extends directly below the pipe string 3. The extended end of the curved pipe 14 is connected to an atomizing ball head 15.

[0003] The method of the shale gas production system for producing shale gas underground is: S1, turn on the variable pump 12, the variable pump 12 draws out the bactericidal corrosion inhibitor in the bactericidal corrosion inhibitor storage tank 13, under the pump pressure, the bactericidal corrosion inhibitor passes through the variable pump 12, the elbow 14, the atomizing ball head 15 in sequence, and finally sprays out from the hole on the atomizing ball head 15, and the sprayed bactericidal corrosion inhibitor enters the pipe column 3; S2, open the end valve 4 of the gas tree and the gas-water separator 5. At this time, the shale gas in the production layer enters the wellbore 1, and then the shale gas under gas pressure flows through the bottom port of the pipe string 3, the inner cavity of the pipe string 3, the gas tree 2, the end valve 4 and finally enters the gas-water separator 5. The flow direction of the shale gas is as follows: Figure 1 As shown by the middle arrow, after the shale gas enters the pipe string 3, the shale gas is mixed with the bactericidal corrosion inhibitor injected into the pipe string 3, and the bactericidal corrosion inhibitor kills the bacteria entrained in the shale gas, thereby effectively preventing bacteria (including iron bacteria, saprophytes and sulfate-reducing bacteria) from corroding the pipe string 3 and causing perforation on the pipe string 3, thereby protecting the pipe string 3; S3, the gas-water separator 5 separates the water entrained in the shale gas, and the separated shale gas without water is transported to the rear section from the gas pipeline 6; and the separated water enters the drainage pipeline 7, and the drainage pipeline 7 discharges the water to the sewage treatment equipment; S4. After a period of shale gas mining, workers need to regularly detect the bacterial content in the pipe column 3. Workers change the displacement of the variable pump 12 according to the detected bacterial content, so as to accurately add the bactericidal corrosion inhibitor into the pipe column 3, thereby protecting the pipe column 3 and saving the amount of bactericidal corrosion inhibitor used. The method for workers to regularly detect the bacterial content in the pipe column 3 is: S41, sampling of sample water: the worker places the sampling container 16 directly below the water outlet pipe 11 of the shale gas extraction system, such as Figure 2 As shown; then open the first stop valve 8, the water in the drainage pipe 7 is diverted to the desander 9 through the first stop valve 8, and then close the first stop valve 8; the desander 9 removes sand and oil in the water, and after a period of rest, the desander 9 purifies the water; then open the second stop valve 10, the purified water in the desander 9 sequentially flows through the second stop valve 10 and the water outlet pipe 11 and finally flows into the sampling container 16, thereby completing the sampling of the sample water; S42, the worker takes out a Figure 3 The filter container shown in the figure comprises a container body 17, an end cover 18 connected to the bottom of the container body 17, a biofilm 19 is fixed between the end cover 18 and the container body 17, and a through groove 20 is opened in the middle of the end cover 18; S43, separating bacteria from sample water: the worker pours the sample water in the sampling container 16 into the container body 17 of the filter container, and the water molecules of the sample water sequentially pass through the biofilm 19 and the through groove 20 and finally fall to the ground. The flow direction of the water molecules is as follows: Figure 4 As shown by the arrow in , the bacteria in the sample water cannot pass through the biofilm 19 and are intercepted on the top surface of the biofilm 19, thereby separating the bacteria in the sample water. At this time, the top surface of the biofilm 19 is covered with a layer of bacteria; S44, the worker inserts the plugging head 21 into the through groove 20 of the end cover 18 to plug the through groove 20, such as Figure 5 Then, the lysis reagent and the stabilizer are sequentially added into the container body 17 of the filtration container, and the adding direction is as shown Figure 6 As shown by the solid arrow in the middle; after adding, the worker shakes the container body 17 to mix the lysis reagent, the stabilizer and the bacteria, wherein the lysis reagent is used to destroy the bacteria to release ATP (adenosine triphosphate) in the bacteria, and the stabilizer is used to stabilize ATP to prevent ATP degradation; S45, the worker adds a certain amount of luciferase reagent into the container body 17 of the filtration container, and the luciferase reagent reacts with ATP to generate a fluorescent substance, which emits light; S46, the worker uses the light shielding cover 22 to cover the top end of the container body 17, such as Figure 7 As shown, to prevent external natural light from entering the container body 17, so as to avoid the detection accuracy of the photometer; and then the photosensitive probe 24 of the photometer 23 is extended into the light shielding cover 22, as shown Figure 8 As shown, the photosensitive probe 24 detects the intensity of light in the container body 17. Since the light intensity is proportional to the bacterial content, the bacterial content in the column 3 is ultimately detected. S47, when the test is completed, the worker dumps the fluorescent substance and residual reagent in the container body 17 of the filter container into the designated waste liquid tank to prepare for the next test; S5. When the detected bacterial content is higher than the specified content, it means that the number of bacteria entering the pipe column 3 is large. At this time, the worker increases the displacement of the variable pump 12, thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3 to prevent bacteria from corroding the pipe column 3, thereby protecting the pipe column 3; when the detected bacterial content is lower than the specified content, it means that the number of bacteria entering the pipe column 3 is small. At this time, the worker reduces the displacement of the variable pump 12, thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3, thereby saving the use of bactericidal corrosion inhibitor.

[0004] However, in step S4, although the workers can detect the bacterial content, in actual operation, there are still the following technical defects: I. In step S43, a worker is required to manually pour the sample water in the sampling container 16 into the container body 17 of the filter container to separate the bacteria in the sample water; in step S44, a worker is required to manually seal the through groove 20 of the end cover 18 with the plugging head 21; in step S44, a worker is required to shake the container body 17 to mix the lysis reagent, stabilizer and bacteria; in steps S44-S45, a worker sequentially adds a lysis reagent, a stabilizer and a luciferase reagent into the container body 17 of the filter container to react and generate fluorescent substances; in step S46, a worker is also required to cover the top port of the container body 17 of the filter container with a light-shielding cover 22, and a worker is also required to extend the photosensitive probe 24 of the photometer 23 into the light-shielding cover 22, so that the light intensity can be detected by the photometer 23, and then the content of bacteria in the column 3 can be finally detected.

[0005] During the entire detection process, all operations are performed manually, and the detection is discontinuous, which not only increases the workload of the workers, but also takes a long time to complete a bacterial content detection, thereby reducing the detection efficiency of the bacterial content in the pipe column 3, and further resulting in the inability to change the displacement of the variable pump 12 in time, and still fails to achieve the purpose of protecting the pipe column 3 and saving the amount of bactericidal corrosion inhibitor used.

[0006] II. In step S47, after the detection is completed, the worker needs to move the filter container to the top of the waste liquid tank, and then pour all the fluorescent substances and residual reagents in the container body 17 into the waste liquid tank. The entire pouring process is also manually operated, which increases the work intensity of the workers.

[0007] Therefore, there is an urgent need for an efficient detection device and method that can reduce the workload of workers, greatly improve the detection efficiency of the bacterial content in the pipe column 3, and can timely change the displacement of the variable pump. Summary of the invention

[0008] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an efficient detection device and method for the bacterial content in the pipe column based on a shale gas extraction system, which reduces the workload of workers, greatly improves the detection efficiency of the bacterial content in the pipe column, and can timely change the displacement of the variable pump.

[0009] The purpose of the present invention is achieved through the following technical solutions: an efficient detection device for the bacterial content in a pipe column based on a shale gas extraction system, which includes a machine supported on the ground, an auxiliary detection component arranged on the machine for collecting sample water, automatically separating bacteria in the sample water, and automatically shaking the reagent, and a liquid adding and light shielding component located on the right side of the auxiliary detection component is also arranged on the machine; The auxiliary detection assembly includes a driving assembly fixed on the machine platform, an L-plate is fixed on the top of the driving assembly, a feed oil cylinder is fixed on the L-plate, a piston rod of the feed oil cylinder penetrates the L-plate to the left and a movable plate is fixed on the extended end, a straight cylinder is hinged to the left side of the movable plate through a hinge seat, a reciprocating oil cylinder is fixed on the right side of the movable plate, a piston rod of the reciprocating oil cylinder penetrates the movable plate to the left and a connecting rod is hinged to the extended end, and the other end of the connecting rod is hinged to the straight cylinder; The left end of the lower ring clamp is welded to the left end of the lower ring clamp, and the left end of the lower ring clamp is welded to the right end of the lower ring clamp. A fixed seat is fixed on the right side wall of the straight cylinder, a hydraulic motor is fixed on the top surface of the fixed seat, the rotating shaft of the hydraulic motor passes through the fixed seat downward, and a vertical oil cylinder is fixed on the extending end, a lifting plate extending to the left is fixed on the acting end of the piston rod of the vertical oil cylinder, and a plug is fixed on the top surface of the lifting plate, which blocks the central hole of the lower annular clamping plate and supports the bottom surface of the biofilm.

[0010] The driving assembly comprises a driving motor fixedly mounted on the machine platform, a vertically mounted lifting cylinder is fixedly mounted on the output shaft of the driving motor, and the L-plate is fixedly mounted on the action end of the piston rod of the lifting cylinder.

[0011] A liquid level sensor is fixedly arranged in the straight cylinder and on the left side wall thereof.

[0012] The middle parts of the upper annular clamping plate and the lower annular clamping plate of the biofilm installation assembly are both provided with a central hole, and the biofilm separates the upper annular clamping plate from the lower annular clamping plate.

[0013] The liquid adding and light shading component includes a bracket fixedly mounted on the machine platform, a lysis reagent storage tank, a stabilizer storage tank and a luciferase reagent storage tank are fixedly mounted on the horizontal plate of the bracket, and the bottom ports of the lysis reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank are all connected with solenoid valves; a light shading tube is also fixedly mounted on the horizontal plate of the bracket, the light shading tube is located on the right side of the luciferase reagent storage tank, the top of the light shading tube is closed, and a photometer is fixedly mounted in the closed end, and the photosensitive probe of the photometer is arranged downward.

[0014] A connecting frame is fixedly arranged between the closed end of the light-shielding tube and the horizontal plate of the bracket.

[0015] A water receiving tank and a waste liquid tank are placed on the table of the machine, wherein the water receiving tank is located directly below the straight tube, and the waste liquid tank is located directly below the light shielding tube.

[0016] The high-efficiency detection device also includes a controller, and the driving motor, hydraulic motor, lifting cylinder, vertical cylinder, reciprocating cylinder, main cylinder, auxiliary cylinder, liquid level sensor and photometer are all electrically connected to the controller via signal lines.

[0017] An efficient method for detecting bacterial content in a pipe column of a shale gas production system comprises the following steps: S1. Sampling of sample water. The specific operation steps are as follows: S11, placing the platform of the detection device on the ground, and ensuring that the straight tube of the detection device is directly below the outlet pipe of the shale gas extraction system; S12, opening the first stop valve, diverting the water in the drainage pipe to the desander through the first stop valve, and then closing the first stop valve; S13, the desander removes sand and oil in the water. After standing for 65 minutes, the desander purifies the water. Then the second stop valve is opened, and the purified water in the desander passes through the second stop valve and the outlet pipe in sequence, and finally flows into the straight cylinder. When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives the electrical signal, the worker immediately closes the second stop valve. At this time, the straight cylinder is filled with sample water, thereby completing the sampling of the sample water. S2. Separate the bacteria from the sample water. The specific steps are as follows: S21, control the piston rod of the lifting cylinder of the auxiliary detection assembly to retract downward, the piston rod drives the L plate to move downward, the L plate drives the feed cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to move downward synchronously, when the piston rod of the lifting cylinder is fully retracted, the straight cylinder is away from the water outlet pipe and close to the water receiving tank; S22, controlling the piston rod of the vertical oil cylinder of the auxiliary detection assembly to extend downward, the piston rod drives the lifting plate to move downward, and the lifting plate drives the plug to move downward, and when the piston rod of the vertical oil cylinder is fully extended, the plug just exits from the central hole of the lower annular clamping plate of the biofilm installation assembly; S23, controlling the rotating shaft of the hydraulic motor of the auxiliary detection component to rotate, and the rotating shaft drives the vertical oil cylinder, the lifting plate and the plug to rotate synchronously. When the plug rotates 90 degrees, the controller controls the hydraulic motor to close. At this time, the water molecules of the sample water in the straight cylinder pass through the biofilm and the central hole of the lower annular clamping plate in sequence, and finally fall into the water receiving tank. The bacteria in the sample water are intercepted on the top surface of the biofilm because they cannot pass through the biofilm, thereby finally separating the bacteria in the sample water. At this time, a layer of bacteria is covered on the top surface of the biofilm; S3, controlling the rotating shaft of the hydraulic motor to rotate in the opposite direction, so that the plug moves to the position directly below the center hole of the lower annular clamping plate; then controlling the piston rod of the vertical oil cylinder to retract upward, so that the plug is inserted into the center hole of the lower annular clamping plate again, and the biofilm is blocked; S4. Reacting the bacteria on the surface of the biofilm into fluorescent substances, the specific operation steps are as follows: S41, control the output shaft of the driving motor to rotate, and the output shaft drives the lifting cylinder, L plate, feeding cylinder, movable plate, straight cylinder and biofilm installation assembly to rotate synchronously. When the straight cylinder rotates 180°, the controller controls the driving motor to turn off. At this time, the straight cylinder just moves to the liquid adding station of the liquid adding and shading component, that is, directly below the lysis reagent storage tank, stabilizer storage tank and luciferase reagent storage tank of the straight cylinder; S42, controlling the piston rod of the lifting cylinder of the auxiliary detection assembly to extend upward, the piston rod drives the L plate to move upward, the L plate drives the feed cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to move upward synchronously, when the piston rod of the lifting cylinder is fully extended, the top end of the straight cylinder is close to the lysis reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank; S43, control the solenoid valve of the lysis reagent storage tank to start, the lysis reagent in the lysis reagent storage tank enters into the straight cylinder through the solenoid valve, and submerges the bacteria on the surface of the biofilm. When the bacteria are added to the set time point, the solenoid valve of the lysis reagent storage tank is closed; then control the solenoid valve of the stabilizer storage tank to start, the stabilizer in the stabilizer storage tank enters into the straight cylinder through the solenoid valve, and when the bacteria are added to the set time point, the solenoid valve of the stabilizer storage tank is closed; S44, controlling the piston rod of the reciprocating cylinder of the auxiliary detection component to perform reciprocating telescopic motion, the piston rod drives the connecting rod to perform left and right reciprocating motion, and the connecting rod drives the straight cylinder to perform reciprocating shaking around the hinge seat, so as to mix the lysis reagent, the stabilizer and the bacteria. During the mixing process, the lysis reagent destroys the bacteria to release the ATP in the bacteria, and the stabilizer stabilizes the ATP to prevent the ATP from degradation; after shaking for 15 minutes, the reciprocating cylinder is controlled to close; S45, controlling the solenoid valve of the luciferase reagent storage tank to start, and the luciferase reagent in the luciferase reagent storage tank enters the straight cylinder through the solenoid valve, and when the luciferase reagent is added to the set time point, the solenoid valve of the luciferase reagent storage tank is closed, at this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thereby finally realizing the reaction of all bacteria on the surface of the biofilm into fluorescent substances; S5. Detection of the light intensity of the light emitted by the fluorescent object in the straight tube. The specific operation steps are as follows: S51, controlling the piston rod of the lifting cylinder of the auxiliary detection assembly to retract downward, the piston rod drives the L plate to move downward, and the L plate drives the feed cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to move downward synchronously; S52, control the piston rod of the feed oil cylinder to extend to the right, the piston rod drives the movable plate to move to the right, the movable plate drives the straight cylinder and the biofilm installation assembly to move to the right synchronously, when the piston rod of the feed oil cylinder is fully extended, the straight cylinder just moves to the bottom of the light-shielding cylinder of the liquid adding and light-shielding assembly; S53, control the piston rod of the lifting cylinder to extend upward, the piston rod drives the L plate to move upward, the L plate drives the straight tube to move upward, when the piston rod of the lifting cylinder is fully extended, the top end of the straight tube is just embedded in the light shielding tube, at this time, the light shielding tube shields the top end of the straight tube to prevent external natural light from entering the container body, at the same time, the photosensitive probe of the photometer enters the straight tube, the photosensitive probe detects the light intensity of the light emitted by the fluorescent object, the photosensitive probe converts the light intensity into an electrical signal, and then the photosensitive probe transmits the electrical signal to the controller, and the controller calculates the content of bacteria in the pipe column according to the electrical signal; When the detected bacterial content is higher than the specified content, it means that the number of bacteria entering the pipe column is large. At this time, the worker increases the displacement of the variable pump, thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column to prevent bacteria from corroding the pipe column, thereby protecting the pipe column; when the detected bacterial content is lower than the specified content, it means that the number of bacteria entering the pipe column is small. At this time, the worker decreases the displacement of the variable pump, thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column, thereby saving the use of bactericidal corrosion inhibitor; S6. After the test is completed, the worker dumps the fluorescent substance and residual reagent in the straight tube into the waste liquid tank in the following steps: S61, controlling the piston rod of the lifting cylinder of the auxiliary detection assembly to retract downward, so that the straight cylinder and the biofilm installation assembly move downward synchronously; S62, control the piston rod of the vertical oil cylinder of the auxiliary detection component to extend downward so that the plug just exits from the center hole of the lower annular clamping plate of the biofilm installation assembly; then control the hydraulic motor to rotate, the hydraulic motor drives the vertical oil cylinder, the lifting plate and the plug to rotate synchronously, when the plug rotates 180°, the controller controls the hydraulic motor to close, at this time, the residual reagent in the straight cylinder passes through the center hole of the upper annular clamping plate, the biofilm, the center hole of the lower annular clamping plate in sequence, and finally falls into the waste liquid tank, thereby realizing the discharge of the residual reagent into the waste liquid tank; S63, the piston rod of the main oil cylinder of the control auxiliary detection component extends downward, the piston rod drives the lifting plate to move downward, the lifting plate drives the auxiliary oil cylinder, the rack, the connecting plate, the gear, the rotating shaft and the biofilm installation assembly to move downward synchronously, when the piston rod of the main oil cylinder is fully extended, the biofilm installation assembly is just separated from the straight cylinder; S64. Control the piston rod of the auxiliary cylinder of the auxiliary detection component to retract upward, the piston rod drives the rack to move upward, the rack drives the gear to rotate, the gear drives the shaft to rotate, and the shaft drives the biofilm installation assembly to rotate synchronously. When the piston rod of the auxiliary cylinder is fully retracted, the biofilm installation assembly is transformed into a vertical state. At this time, the fluorescent substance on the surface of the biofilm falls into the waste liquid tank through the center hole of the upper annular clamp, thereby dumping the fluorescent substance into the waste liquid tank to prepare for the second bacterial content.

[0018] The present invention has the following advantages: reducing the work intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe column, and being able to change the displacement of the variable pump in time. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the shale gas extraction system; Figure 2 A diagram showing a worker placing a sampling container just below the outlet pipe of a shale gas extraction system; Figure 3 is a schematic diagram of the structure of the filter container; Figure 4 A schematic diagram of a worker pouring sample water from a sampling container into a container body of a filtering container; Figure 5 A schematic diagram of a worker inserting a plugging head into a through groove of an end cover; Figure 6 A schematic diagram of a worker sequentially adding a lysis reagent and a stabilizer into a container body of a filtration container; Figure 7 A schematic diagram of a worker shielding the top port of a container body with a light-shielding cover; Figure 8 A schematic diagram of a worker inserting the light-sensitive probe of a photometer into the light-shielding cover; Fig. 9 It is a structural schematic diagram of the present invention; Fig.10 It is a schematic diagram of the structure of the auxiliary detection component; Fig.11 for Fig.10 A-direction schematic diagram; Fig.12 for Fig.10 The main cross-sectional diagram of Fig.13 for Fig.12 A schematic diagram of the connection between the biofilm installation assembly, the rotating shaft and the gear; Fig.14 for Fig.13 A top view of Fig.15 It is a structural schematic diagram of the liquid adding and shading components; Fig.16 It is a schematic diagram of the straight cylinder of the present invention being located directly below the water outlet pipe of the shale gas production system; Fig.17 It is a schematic diagram showing that the straight tube is far away from the water outlet pipe; Fig.18 A schematic diagram of a plug withdrawing from a central hole of a lower annular clamping plate of a biofilm mounting assembly; Fig.19 Schematic diagram of the blockage rotated 90°; Fig. 20 It is a schematic diagram of the straight cylinder moving to the liquid adding station of the liquid adding and light shielding assembly; Fig.21 The top end of the straight cylinder is close to the lysis reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank; Fig. 22 It is a schematic diagram of the straight cylinder moving to the bottom of the light-shielding cylinder of the liquid-adding and light-shielding assembly; Fig.23 It is a schematic diagram of the top port of the straight tube being embedded in the light-shielding tube; Fig.24 Schematic diagram of the blockage rotated 180°; Fig.25 Schematic diagram of the separation of the biofilm installation assembly and the straight cylinder; Fig.26 A schematic diagram of the biofilm installation assembly being transformed into a vertical state; In the figure: 1-wellbore, 2-gas tree, 3-pipe string, 4-end valve, 5-gas-water separator, 6-gas pipeline, 7-drainage pipeline, 8-first stop valve, 9-sander, 10-second stop valve, 11-water outlet pipe, 12-variable pump, 13-bactericide and corrosion inhibitor storage tank, 14-elbow, 15-atomizing ball head; 16-sampling container, 17-container body, 18-end cover, 19-biofilm, 20-through slot, 21-plugging head, 22-light shielding cover, 23-photometer, 24-photosensitive probe; 25-machine table, 26-auxiliary detection component, 27-liquid adding and shading component, 28-L plate, 29-feeding cylinder, 30-movable plate, 31-hinge seat, 32-straight cylinder, 33-reciprocating cylinder, 34-connecting rod; 35-main oil cylinder, 36-lifting plate, 37-auxiliary oil cylinder, 38-rack, 39-connecting plate, 40-rotating shaft, 41-gear; 42-lower annular clamping plate, 43-upper annular clamping plate, 44-hydraulic motor, 45-vertical oil cylinder, 46-lifting plate, 47-blocking, 48-driving motor, 49-lifting oil cylinder; 50- bracket, 51- lysis reagent storage tank, 52- stabilizer storage tank, 53- luciferase reagent storage tank, 54- light shielding tube; 55- water receiving tank, 56- waste liquid tank. DETAILED DESCRIPTION

[0020] The present invention is further described below in conjunction with the accompanying drawings, and the protection scope of the present invention is not limited to the following: like Fig. 9 As shown, a highly efficient detection device for the bacterial content in a pipe column of a shale gas extraction system includes a machine platform 25 supported on the ground, an auxiliary detection component 26 arranged on the machine platform 25 for collecting sample water, automatically separating bacteria in the sample water, and automatically shaking the reagent, and a liquid adding and shading component 27 located on the right side of the auxiliary detection component 26 is also arranged on the machine platform 25.

[0021] like Figure 10~Figure 14 As shown, the auxiliary detection component 26 includes a drive assembly fixed on the machine 25, an L plate 28 is fixed on the top of the drive assembly, a feed cylinder 29 is fixed on the L plate 28, the piston rod of the feed cylinder 29 passes through the L plate 28 to the left and a movable plate 30 is fixed on the extended end, a straight cylinder 32 is hinged on the left side of the movable plate 30 through a hinge seat 31, a reciprocating cylinder 33 is fixed on the right side of the movable plate 30, the piston rod of the reciprocating cylinder 33 passes through the movable plate 30 to the left and a connecting rod 34 is hinged on the extended end, and the other end of the connecting rod 34 is hinged on the straight cylinder 32; a liquid level sensor is fixed in the straight cylinder 32 and on its left side wall. The drive assembly includes a drive motor 48 fixed on the machine 25, a vertically arranged lifting cylinder 49 is fixed on the output shaft of the drive motor 48, and the L plate 28 is fixed on the action end of the piston rod of the lifting cylinder 49.

[0022] like Figure 10~Figure 14As shown, a main oil cylinder 35 is fixedly arranged on the left side wall of the straight cylinder 32, a lifting plate 36 is fixedly arranged on the piston rod of the main oil cylinder 35, a sub-oil cylinder 37 is fixedly arranged on the top surface of the lifting plate 36, a piston rod of the sub-oil cylinder 37 penetrates downwardly through the lifting plate 36 and a rack 38 is fixedly arranged on the extended end, a connecting plate 39 is fixedly arranged on the bottom surface of the lifting plate 36, a rotating shaft 40 is rotatably installed in the lower end of the connecting plate 39, a gear 41 is installed on the left end of the rotating shaft 40, the gear 41 is meshed with the rack 38, and a gear 41 is arranged on the right end of the rotating shaft 40 directly below the straight cylinder 32 The biofilm installation assembly includes a lower annular clamping plate 42, a biofilm 19 and an upper annular clamping plate 43 stacked in sequence from bottom to top. A plurality of locking screws are connected to the outer edges of the upper annular clamping plate 43 and the lower annular clamping plate 42. Under the threaded connection force of the locking screws, the outer edge of the biofilm 19 is clamped and fixed between the upper annular clamping plate 43 and the lower annular clamping plate 42. The top surface of the upper annular clamping plate 43 presses against the sealing ring located on the bottom surface of the straight cylinder 32. The left side wall of the lower annular clamping plate 42 is welded to the right end of the rotating shaft 40. The upper annular clamping plate 43 and the lower annular clamping plate 42 of the biofilm installation assembly are both provided with a central hole in the middle, and the biofilm 19 separates the upper annular clamping plate 43 from the lower annular clamping plate 42.

[0023] A fixed seat is fixed on the right side wall of the straight cylinder 32, and a hydraulic motor 44 is fixed on the top surface of the fixed seat. The rotating shaft of the hydraulic motor 44 passes through the fixed seat downward, and a vertical cylinder 45 is fixed on the extending end. A lifting plate 46 extending to the left is fixed on the acting end of the piston rod of the vertical cylinder 45, and a plug 47 is fixed on the top surface of the lifting plate 46. The plug 47 blocks the central hole of the lower annular clamp 42 and supports the bottom surface of the biofilm 19.

[0024] like Fig.15 As shown, the liquid adding and light shielding assembly 27 includes a bracket 50 fixedly mounted on the machine platform 25, and a lysis reagent storage tank 51, a stabilizer storage tank 52 and a luciferase reagent storage tank 53 are fixedly mounted on the horizontal plate of the bracket 50, and the bottom ports of the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53 are all connected with electromagnetic valves; a light shielding tube 54 is also fixedly mounted on the horizontal plate of the bracket 50, and the light shielding tube 54 is located on the right side of the luciferase reagent storage tank 53, and the top of the light shielding tube 54 is closed, and a photometer 23 is fixedly mounted in the closed end, and the photosensitive probe 24 of the photometer 23 is arranged downward. A connecting frame is fixedly mounted between the closed end of the light shielding tube 54 and the horizontal plate of the bracket 50.

[0025] A water receiving tank 55 and a waste liquid tank 56 are placed on the table of the machine table 25 . The water receiving tank 55 is located directly below the straight tube 32 , and the waste liquid tank 56 is located directly below the light shielding tube 54 .

[0026] The high-efficiency detection device also includes a controller. The drive motor 48, hydraulic motor 44, lifting cylinder 49, vertical cylinder 45, reciprocating cylinder 33, main cylinder 35, auxiliary cylinder 37, liquid level sensor and photometer 23 are all electrically connected to the controller via signal lines. Workers can control the start or stop of the drive motor 48 and hydraulic motor 44 through the controller. At the same time, they can also control the extension or retraction of the piston rods of the lifting cylinder 49, vertical cylinder 45, reciprocating cylinder 33, main cylinder 35 and auxiliary cylinder 37, thereby facilitating the workers' operation and having the characteristics of a high degree of automation.

[0027] An efficient method for detecting bacterial content in a pipe column of a shale gas production system comprises the following steps: S1. Sampling of sample water. The specific operation steps are as follows: S11, place the platform 25 of the detection device on the ground, and ensure that the straight tube 32 of the detection device is in a Figure 1 Directly below the outlet pipe 11 of the shale gas production system shown in FIG. Fig.16 As shown; S12, opening the first stop valve 8, the water in the drainage pipe 7 is diverted to the desander 9 through the first stop valve 8, and then the first stop valve 8 is closed; S13, the desander 9 removes sand and oil in the water. After standing for 65 minutes, the desander 9 purifies the water. Then the second stop valve 10 is opened, and the purified water in the desander 9 passes through the second stop valve 10 and the water outlet pipe 11 in sequence, and finally flows into the straight cylinder 32. When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives the electrical signal, the worker immediately closes the second stop valve 10. At this time, the straight cylinder 32 is filled with sample water, thereby completing the sampling of the sample water. S2. Separate the bacteria from the sample water. The specific steps are as follows: S21, control the piston rod of the lifting cylinder 49 of the auxiliary detection component 26 to retract downward, the piston rod drives the L plate 28 to move downward, the L plate 28 drives the feed cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to move downward synchronously, when the piston rod of the lifting cylinder 49 is fully retracted, the straight cylinder 32 is away from the water outlet pipe 11, as shown in FIG. Fig.17 As shown, and close to the water receiving tank 55; S22, control the piston rod of the vertical oil cylinder 45 of the auxiliary detection component 26 to extend downward, the piston rod drives the lifting plate 46 to move downward, and the lifting plate 46 drives the plug 47 to move downward. When the piston rod of the vertical oil cylinder 45 is fully extended, the plug 47 just withdraws from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly, as shown in FIG. Fig.18 As shown; S23, control the rotation shaft of the hydraulic motor 44 of the auxiliary detection component 26 to rotate, and the rotation shaft drives the vertical oil cylinder 45, the lifting plate 46 and the plug 47 to rotate synchronously. When the plug 47 rotates 90°, as shown in FIG. Fig.19 As shown, the controller controls the hydraulic motor 44 to be turned off. At this time, the water molecules of the sample water in the straight cylinder 32 pass through the biofilm 19 and the central hole of the lower annular clamping plate 42 in sequence, and finally fall into the water receiving tank 55. The movement direction of the water molecules is as shown in FIG. Fig.19 As shown by the hollow arrow, the bacteria in the sample water are intercepted on the top surface of the biofilm 19 because they cannot pass through the biofilm 19, thereby finally separating the bacteria in the sample water. At this time, the top surface of the biofilm 19 is covered with a layer of bacteria; S3, control the rotary shaft of the hydraulic motor 44 to rotate in the opposite direction, so that the plug 47 moves to the position directly below the center hole of the lower annular clamping plate 42; then control the piston rod of the vertical oil cylinder 45 to retract upward, so that the plug 47 is inserted into the center hole of the lower annular clamping plate 42 again, and the biofilm 19 is blocked; S4, reacting the bacteria on the surface of the biofilm 19 into fluorescent substances, the specific operation steps are as follows: S41, control the output shaft of the driving motor 48 to rotate, and the output shaft drives the lifting cylinder 49, the L plate 28, the feeding cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to rotate synchronously. When the straight cylinder 32 rotates 180°, the controller controls the driving motor 48 to turn off. At this time, the straight cylinder 32 just moves to the liquid adding station of the liquid adding and shading component 27, such as Fig. 20 As shown, that is, directly below the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53 of the straight cylinder 32; S42, the piston rod of the lifting cylinder 49 of the control auxiliary detection component 26 is extended upward, the piston rod drives the L plate 28 to move upward, and the L plate 28 drives the feed cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to move upward synchronously. When the piston rod of the lifting cylinder 49 is fully extended, the top end of the straight cylinder 32 is close to the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53, as shown in FIG. Fig.21 As shown; S43, control the solenoid valve of the lysis reagent storage tank 51 to start, the lysis reagent in the lysis reagent storage tank 51 enters into the straight cylinder 32 through the solenoid valve, and submerges the bacteria on the surface of the biofilm 19. When the bacteria are added to the set time point, the solenoid valve of the lysis reagent storage tank 51 is closed; then control the solenoid valve of the stabilizer storage tank 52 to start, the stabilizer in the stabilizer storage tank 52 enters into the straight cylinder 32 through the solenoid valve. When the bacteria are added to the set time point, the solenoid valve of the stabilizer storage tank 52 is closed; S44, control the piston rod of the reciprocating cylinder 33 of the auxiliary detection component 26 to perform reciprocating telescopic motion, the piston rod drives the connecting rod 34 to perform left and right reciprocating motion, the connecting rod 34 drives the straight tube 32 to perform reciprocating shaking around the hinge seat 31, and the shaking direction of the straight tube 32 is as follows: Fig.21 As shown by the arrow in the figure, the lysis reagent, the stabilizer and the bacteria are mixed. During the mixing process, the lysis reagent destroys the bacteria to release the ATP in the bacteria, while the stabilizer stabilizes the ATP to prevent the ATP from being degraded. After shaking for 15 minutes, the reciprocating cylinder 33 is controlled to be closed. S45, the solenoid valve of the luciferase reagent storage tank 53 is controlled to start, and the luciferase reagent in the luciferase reagent storage tank 53 enters the straight cylinder 32 through the solenoid valve. When the luciferase reagent is added to the set time point, the solenoid valve of the luciferase reagent storage tank 53 is closed. At this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thereby finally realizing the reaction of all bacteria on the surface of the biofilm 19 into fluorescent substances; S5, detection of the light intensity of the light emitted by the fluorescent substance in the straight tube 32, the specific operation steps are as follows: S51, control the piston rod of the lifting cylinder 49 of the auxiliary detection assembly 26 to retract downward, the piston rod drives the L plate 28 to move downward, and the L plate 28 drives the feed cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to move downward synchronously; S52, control the piston rod of the feed cylinder 29 to extend to the right, the piston rod drives the movable plate 30 to move to the right, the movable plate 30 drives the straight cylinder 32 and the biofilm installation assembly to move to the right synchronously, when the piston rod of the feed cylinder 29 is fully extended, the straight cylinder 32 just moves to the bottom of the light-shielding cylinder 54 of the liquid-adding and light-shielding assembly 27, as shown in FIG. Fig. 22 As shown; S53, control the piston rod of the lifting cylinder 49 to extend upward, the piston rod drives the L plate 28 to move upward, and the L plate 28 drives the straight tube 32 to move upward. When the piston rod of the lifting cylinder 49 is fully extended, the top end of the straight tube 32 is just embedded in the light shielding tube 54, as shown in FIG. Fig.23 As shown, at this time, the light shielding tube 54 blocks the top port of the straight tube 32 to prevent external natural light from entering the container body 17. At the same time, the photosensitive probe 24 of the photometer 23 enters the straight tube 32, and the photosensitive probe 24 detects the light intensity of the light emitted by the fluorescent object. The photosensitive probe 24 converts the light intensity into an electrical signal, and then the photosensitive probe 24 transmits the electrical signal to the controller, and the controller calculates the content of bacteria in the column 3 according to the electrical signal; When the detected bacterial content is higher than the specified content, it means that the number of bacteria entering the pipe column 3 is large. At this time, the worker increases the displacement of the variable pump 12, thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3 to prevent bacteria from corroding the pipe column 3, thereby protecting the pipe column 3; when the detected bacterial content is lower than the specified content, it means that the number of bacteria entering the pipe column 3 is small. At this time, the worker reduces the displacement of the variable pump 12, thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3, thereby saving the use of bactericidal corrosion inhibitor.

[0028] Among them, from step S2, the detection device only needs to cooperate with the vertical cylinder 45 and the hydraulic motor 44 of the auxiliary detection component 26 to withdraw the plug 47 from the central hole of the lower annular clamp 42 of the biofilm installation assembly, thereby automatically separating the bacteria in the sample water; in step S4, the detection device only needs to cooperate with the auxiliary detection component 26, the liquid adding and the light shielding component 27 to automatically add the lysis reagent, the stabilizer and the luciferase reagent into the straight cylinder 32, and can also automatically shake the straight cylinder 32 to mix the lysis reagent, the stabilizer and the bacteria, thereby releasing the ATP in the bacteria; in step S5, the detection device only needs to cooperate with the auxiliary detection component 26, the liquid adding and the light shielding component 27 to automatically cover the light shielding cylinder 54 on the top port of the straight cylinder 32, and extend the photosensitive probe 24 of the photometer 23 into the straight cylinder 32, thereby detecting the light intensity, and then detecting the content of bacteria in the column 3; It can be seen that this detection device is better than Figure 2~Figure 8 The detection method shown does not require workers to manually pour the sample water in the sampling container 16 into the container body 17 of the filter container in order to separate the bacteria in the sample water; nor does it require workers to shake the container body 17 to mix the lysis reagent, stabilizer and bacteria; nor does it require workers to sequentially add the lysis reagent, stabilizer and luciferase reagent into the container body 17 of the filter container to react and generate fluorescent substances; nor does it require workers to cover the top port of the container body 17 of the filter container with the light-shielding cover 22, nor does it require workers to extend the photosensitive probe 24 of the photometer 23 into the light-shielding cover 22 in order to detect the light intensity with the photometer 23.

[0029] The detection device realizes automatic and continuous detection, which not only greatly reduces the work intensity of workers, but also completes a bacterial content detection in a short time, thereby greatly improving the detection efficiency of the bacterial content in the pipe column 3, so that the displacement of the variable pump 12 can be changed in time, thereby protecting the pipe column 3 and saving the use of bactericidal corrosion inhibitors.

[0030] S6. After the test is completed, the worker dumps the fluorescent substance and residual reagent in the straight tube 32 into the waste liquid tank 56 in the following steps: S61, controlling the piston rod of the lifting cylinder 49 of the auxiliary detection assembly 26 to retract downward, so that the straight cylinder 32 and the biofilm installation assembly move downward synchronously; S62, control the piston rod of the vertical cylinder 45 of the auxiliary detection assembly 26 to extend downward so that the plug 47 just exits the center hole of the lower annular clamping plate 42 of the biofilm installation assembly; then control the hydraulic motor 44 to rotate, the hydraulic motor 44 drives the vertical cylinder 45, the lifting plate 46 and the plug 47 to rotate synchronously, and when the plug 47 rotates 180°, as shown in FIG. Fig.24 As shown, the controller controls the hydraulic motor 44 to be turned off. At this time, the residual reagent in the straight cylinder 32 passes through the central hole of the upper annular clamping plate 43, the biofilm 19, the central hole of the lower annular clamping plate 42 in sequence, and finally falls into the waste liquid tank 56. The movement direction of the residual reagent is as shown in FIG. Fig.24 As shown by the arrow in , the residual reagent is discharged into the waste liquid tank 56; S63, the piston rod of the main oil cylinder 35 of the control auxiliary detection component 26 extends downward, the piston rod drives the lifting plate 36 to move downward, the lifting plate 36 drives the auxiliary oil cylinder 37, the rack 38, the connecting plate 39, the gear 41, the rotating shaft 40 and the biofilm installation assembly to move downward synchronously, when the piston rod of the main oil cylinder 35 is fully extended, the biofilm installation assembly is just separated from the straight cylinder 32, as shown in FIG. Fig.25 As shown; S64, control the piston rod of the auxiliary oil cylinder 37 of the auxiliary detection component 26 to retract upward, the piston rod drives the rack 38 to move upward, the rack 38 drives the gear 41 to rotate, the gear 41 drives the shaft 40 to rotate, the shaft 40 drives the biofilm installation assembly to rotate synchronously, when the piston rod of the auxiliary oil cylinder 37 is fully retracted, the biofilm installation assembly is transformed into a vertical state, such as Fig.26 As shown, at this time, the fluorescent substance on the surface of the biofilm 19 falls into the waste liquid tank 56 through the central hole of the upper annular clamping plate 43, thereby pouring the fluorescent substance into the waste liquid tank 56 to prepare for the second bacterial content.

[0031] Among them, it can be seen from step S6 that the detection device only needs to control the piston rod of the vertical oil cylinder 45 to extend downward so that the plug 47 just withdraws from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly, thereby realizing the discharge of the residual reagent in the straight cylinder 32 into the waste liquid tank 56; and then control the linkage cooperation of the main oil cylinder 35 and the auxiliary oil cylinder 37 to transform the biofilm installation assembly into a vertical state, thereby realizing the dumping of the fluorescent substance on the surface of the biofilm 19 into the waste liquid tank 56. It can be seen that the detection device realizes the automatic dumping of all the fluorescent substances and residual reagents in the straight cylinder 32 into the waste liquid tank 56, without manual dumping, thereby greatly reducing the work intensity of the workers.

Claims

1. An efficient detection device for bacterial content in a pipe column of a shale gas production system, characterized in that: The device comprises a machine platform (25) supported on the ground, an auxiliary detection component (26) arranged on the machine platform (25) for collecting sample water, automatically separating bacteria in the sample water, and automatically shaking the reagent, and a liquid adding and light shielding component (27) located on the right side of the auxiliary detection component (26) is also arranged on the machine platform (25); The auxiliary detection component (26) comprises a driving assembly fixedly mounted on the machine platform (25), an L-plate (28) fixedly mounted on the top of the driving assembly, a feed cylinder (29) fixedly mounted on the L-plate (28), a piston rod of the feed cylinder (29) passing through the L-plate (28) to the left and a movable plate (30) fixedly mounted on the extended end, a straight cylinder (32) hingedly mounted on the left side of the movable plate (30) via a hinge seat (31), a reciprocating cylinder (33) fixedly mounted on the right side of the movable plate (30), a piston rod of the reciprocating cylinder (33) passing through the movable plate (30) to the left and a connecting rod (34) hingedly mounted on the extended end, and the other end of the connecting rod (34) hingedly mounted on the straight cylinder (32); A main oil cylinder (35) is fixedly provided on the left side wall of the straight cylinder (32); a lifting plate (36) is fixedly provided on the piston rod of the main oil cylinder (35); a sub-oil cylinder (37) is fixedly provided on the top surface of the lifting plate (36); a piston rod of the sub-oil cylinder (37) passes through the lifting plate (36) downward and a rack (38) is fixedly provided on the extended end; a connecting plate (39) is fixedly provided on the bottom surface of the lifting plate (36); a rotating shaft (40) is rotatably installed in the lower end of the connecting plate (39); a gear (41) is installed on the left end of the rotating shaft (40); the gear (41) is meshed with the rack (38); a gear (41) is provided on the right end of the rotating shaft (40) and is located in the straight cylinder ( 32), the biofilm installation assembly comprising a lower annular clamping plate (42), a biofilm (19) and an upper annular clamping plate (43) stacked in sequence from bottom to top, a plurality of locking screws being connected at the outer edges of the upper annular clamping plate (43) and the lower annular clamping plate (42), under the threaded connection force of the locking screws, the outer edge of the biofilm (19) is clamped and fixed between the upper annular clamping plate (43) and the lower annular clamping plate (42), the top surface of the upper annular clamping plate (43) is pressed against the sealing ring located on the bottom surface of the straight cylinder (32), and the left side wall of the lower annular clamping plate (42) is welded to the right end of the rotating shaft (40); A fixed seat is fixedly provided on the right side wall of the straight cylinder (32), and a hydraulic motor (44) is fixedly provided on the top surface of the fixed seat. The rotating shaft of the hydraulic motor (44) passes through the fixed seat downward, and a vertical oil cylinder (45) is fixedly provided on the extended end. A lifting plate (46) extending to the left is fixedly provided on the action end of the piston rod of the vertical oil cylinder (45), and a plug (47) is fixedly provided on the top surface of the lifting plate (46). The plug (47) blocks the central hole of the lower annular clamp (42) and supports the bottom surface of the biofilm (19).

2. According to claim 1, a highly efficient detection device for bacteria content in a pipe column of a shale gas production system is characterized by: The drive assembly comprises a drive motor (48) fixedly mounted on the machine platform (25); a vertically mounted lifting cylinder (49) is fixedly mounted on the output shaft of the drive motor (48); and the L-plate (28) is fixedly mounted on the action end of the piston rod of the lifting cylinder (49).

3. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 2, characterized in that: A liquid level sensor is fixedly arranged in the straight cylinder (32) and on its left side wall.

4. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 3 is characterized by: The upper annular clamping plate (43) and the lower annular clamping plate (42) of the biofilm installation assembly are both provided with a central hole in the middle, and the biofilm (19) separates the upper annular clamping plate (43) from the lower annular clamping plate (42).

5. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 4 is characterized in that: The liquid adding and light shielding component (27) comprises a bracket (50) fixedly mounted on the machine platform (25); a lysis reagent storage tank (51), a stabilizer storage tank (52) and a luciferase reagent storage tank (53) are fixedly mounted on a horizontal plate of the bracket (50); and the bottom ports of the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53) are all connected to electromagnetic valves; a light shielding tube (54) is also fixedly mounted on the horizontal plate of the bracket (50); the light shielding tube (54) is located on the right side of the luciferase reagent storage tank (53); the top of the light shielding tube (54) is closed, and a photometer (23) is fixedly mounted in the closed end; and the photosensitive probe (24) of the photometer (23) is arranged downward.

6. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 5, characterized in that: A connecting frame is fixedly provided between the closed end of the light-shielding tube (54) and the horizontal plate of the bracket (50).

7. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 6, characterized in that: A water receiving trough (55) and a waste liquid trough (56) are placed on the table top of the machine table (25); the water receiving trough (55) is located directly below the straight tube (32), and the waste liquid trough (56) is located directly below the light shielding tube (54).

8. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 7, characterized in that: The high-efficiency detection device also includes a controller, wherein the drive motor (48), the hydraulic motor (44), the lifting cylinder (49), the vertical cylinder (45), the reciprocating cylinder (33), the main cylinder (35), the auxiliary cylinder (37), the liquid level sensor and the photometer (23) are all electrically connected to the controller via signal lines.

9. A method for efficiently detecting the bacterial content in a pipe column of a shale gas production system, using the device for efficiently detecting the bacterial content in a pipe column of a shale gas production system according to claim 8, characterized in that: It includes the following steps: S1. Sampling of sample water. The specific operation steps are as follows: S11, placing the platform (25) of the detection device on the ground, and ensuring that the straight cylinder (32) of the detection device is directly below the water outlet pipe (11) of the shale gas extraction system; S12, opening the first stop valve (8), so that the water in the drainage pipe (7) is diverted to the desander (9) through the first stop valve (8), and then the first stop valve (8) is closed; S13, the desander (9) removes sand and oil from the water. After standing still for 65 minutes, the desander (9) has completely purified the water. Then, the second stop valve (10) is opened, and the purified water in the desander (9) passes through the second stop valve (10) and the water outlet pipe (11) in sequence, and finally flows into the straight cylinder (32). When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives the electrical signal, the worker immediately closes the second stop valve (10). At this time, the straight cylinder (32) is filled with sample water, thereby completing the sampling of the sample water. S2. Separate the bacteria from the sample water. The specific steps are as follows: S21, the piston rod of the lifting cylinder (49) of the auxiliary detection component (26) is controlled to retract downward, the piston rod drives the L plate (28) to move downward, and the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move downward synchronously. When the piston rod of the lifting cylinder (49) is fully retracted, the straight cylinder (32) is separated from the water outlet pipe (11) and approaches the water receiving tank (55); S22, controlling the piston rod of the vertical oil cylinder (45) of the auxiliary detection component (26) to extend downward, the piston rod drives the lifting plate (46) to move downward, and the lifting plate (46) drives the plug (47) to move downward, and when the piston rod of the vertical oil cylinder (45) is fully extended, the plug (47) just exits from the center hole of the lower annular clamping plate (42) of the biofilm installation assembly; S23, controlling the rotation shaft of the hydraulic motor (44) of the auxiliary detection component (26) to rotate, and the rotation shaft drives the vertical oil cylinder (45), the lifting plate (46) and the plug (47) to rotate synchronously. When the plug (47) rotates 90 degrees, the controller controls the hydraulic motor (44) to close. At this time, the water molecules of the sample water in the straight cylinder (32) pass through the biofilm (19) and the central hole of the lower annular clamping plate (42) in sequence, and finally fall into the water receiving tank (55). The bacteria in the sample water cannot pass through the biofilm (19) and are intercepted on the top surface of the biofilm (19), thereby finally achieving the separation of the bacteria in the sample water. At this time, the top surface of the biofilm (19) is covered with a layer of bacteria; S3, controlling the rotating shaft of the hydraulic motor (44) to rotate in the opposite direction, so that the plug (47) moves to the position directly below the center hole of the lower annular clamp (42); then controlling the piston rod of the vertical oil cylinder (45) to retract upward, so that the plug (47) is inserted back into the center hole of the lower annular clamp (42), thereby blocking the biofilm (19); S4. Reacting the bacteria on the surface of the biofilm (19) into fluorescent substances, the specific operation steps are as follows: S41, controlling the output shaft of the driving motor (48) to rotate, and the output shaft drives the lifting cylinder (49), the L plate (28), the feeding cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to rotate synchronously. When the straight cylinder (32) rotates 180°, the controller controls the driving motor (48) to turn off. At this time, the straight cylinder (32) just moves to the liquid adding station of the liquid adding and shading component (27), that is, directly below the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53) of the straight cylinder (32); S42, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection assembly (26) to extend upward, the piston rod drives the L plate (28) to move upward, the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move upward synchronously, and when the piston rod of the lifting cylinder (49) is fully extended, the top end of the straight cylinder (32) is close to the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53); S43, controlling the solenoid valve of the lysis reagent storage tank (51) to start, the lysis reagent in the lysis reagent storage tank (51) enters into the straight cylinder (32) through the solenoid valve, and submerges the bacteria on the surface of the biofilm (19), and when the bacteria are added to the set time point, the solenoid valve of the lysis reagent storage tank (51) is closed; and then controlling the solenoid valve of the stabilizer storage tank (52) to start, the stabilizer in the stabilizer storage tank (52) enters into the straight cylinder (32) through the solenoid valve, and when the bacteria are added to the set time point, the solenoid valve of the stabilizer storage tank (52) is closed; S44, controlling the piston rod of the reciprocating cylinder (33) of the auxiliary detection component (26) to perform reciprocating telescopic motion, the piston rod drives the connecting rod (34) to perform left and right reciprocating motion, the connecting rod (34) drives the straight cylinder (32) to perform reciprocating shaking around the hinge seat (31), thereby mixing the lysis reagent, the stabilizer and the bacteria. During the mixing process, the lysis reagent destroys the bacteria to release ATP in the bacteria, while the stabilizer stabilizes the ATP to prevent ATP degradation; after shaking for 15 minutes, controlling the reciprocating cylinder (33) to close; S45, controlling the solenoid valve of the luciferase reagent storage tank (53) to start, and the luciferase reagent in the luciferase reagent storage tank (53) enters the straight cylinder (32) through the solenoid valve. When the luciferase reagent is added to the set time point, the solenoid valve of the luciferase reagent storage tank (53) is closed. At this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thereby finally achieving the reaction of all bacteria on the surface of the biofilm (19) into fluorescent substances; S5, detecting the light intensity of the light emitted by the fluorescent substance in the straight tube (32), the specific operation steps are as follows: S51, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection component (26) to retract downward, the piston rod drives the L plate (28) to move downward, and the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move downward synchronously; S52, the piston rod of the control feed cylinder (29) is extended to the right, the piston rod drives the movable plate (30) to move to the right, the movable plate (30) drives the straight cylinder (32) and the biofilm installation assembly to move to the right synchronously, and when the piston rod of the feed cylinder (29) is fully extended, the straight cylinder (32) moves just below the light shielding cylinder (54) of the liquid adding and light shielding assembly (27); S53, control the piston rod of the lifting cylinder (49) to extend upward, the piston rod drives the L plate (28) to move upward, the L plate (28) drives the straight tube (32) to move upward, when the piston rod of the lifting cylinder (49) is fully extended, the top end of the straight tube (32) is just embedded in the light shielding tube (54), at this time, the light shielding tube (54) shields the top end of the straight tube (32) to prevent external natural light from entering the container body (17), at the same time, the photosensitive probe (24) of the photometer (23) enters the straight tube (32), the photosensitive probe (24) detects the light intensity of the light emitted by the fluorescent object, the photosensitive probe (24) converts the light intensity into an electrical signal, and then the photosensitive probe (24) transmits the electrical signal to the controller, and the controller calculates the content of bacteria in the pipe column (3) according to the electrical signal; When the detected bacterial content is higher than the specified content, it means that the number of bacteria entering the pipe column (3) is large. At this time, the worker increases the displacement of the variable pump (12), thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column (3) to prevent bacteria from corroding the pipe column (3), thereby protecting the pipe column (3); when the detected bacterial content is lower than the specified content, it means that the number of bacteria entering the pipe column (3) is small. At this time, the worker decreases the displacement of the variable pump (12), thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column (3), thereby saving the amount of bactericidal corrosion inhibitor used; S6. After the test is completed, the worker dumps the fluorescent substance and residual reagent in the straight cylinder (32) into the waste liquid tank (56). The operation steps are as follows: S61, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection assembly (26) to retract downward, thereby causing the straight cylinder (32) and the biofilm installation assembly to move downward synchronously; S62, controlling the piston rod of the vertical oil cylinder (45) of the auxiliary detection component (26) to extend downward so that the plug (47) just exits from the center hole of the lower annular clamp (42) of the biofilm installation assembly; then controlling the hydraulic motor (44) to rotate, the hydraulic motor (44) drives the vertical oil cylinder (45), the lifting plate (46) and the plug (47) to rotate synchronously, and when the plug (47) rotates 180°, the controller controls the hydraulic motor (44) to turn off, at which time, the residual reagent in the straight cylinder (32) passes through the center hole of the upper annular clamp (43), the biofilm (19), the center hole of the lower annular clamp (42), and finally falls into the waste liquid tank (56), thereby achieving the discharge of the residual reagent into the waste liquid tank (56); S63, the piston rod of the main oil cylinder (35) of the control auxiliary detection component (26) extends downward, the piston rod drives the lifting plate (36) to move downward, the lifting plate (36) drives the auxiliary oil cylinder (37), the rack (38), the connecting plate (39), the gear (41), the rotating shaft (40) and the biofilm installation assembly to move downward synchronously, and when the piston rod of the main oil cylinder (35) is fully extended, the biofilm installation assembly is just separated from the straight cylinder (32); S64, the piston rod of the auxiliary cylinder (37) of the control auxiliary detection component (26) is retracted upward, the piston rod drives the rack (38) to move upward, the rack (38) drives the gear (41) to rotate, the gear (41) drives the shaft (40) to rotate, and the shaft (40) drives the biofilm installation assembly to rotate synchronously. When the piston rod of the auxiliary cylinder (37) is fully retracted, the biofilm installation assembly is transformed into a vertical state. At this time, the fluorescent substance on the surface of the biofilm (19) falls into the waste liquid tank (56) through the center hole of the upper annular clamp (43), thereby dumping the fluorescent substance into the waste liquid tank (56) to prepare for the second bacterial content.

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