Neospirillum deserticola CDCHPC1 of fish source of floating islands and application of Neospirillum deserticola CDCHPC1 in purification of inorganic nitrogen and phosphorus in water

Abstract

The invention discloses neospirillum deserticola CDCHPC1 of an symmetry island fish source and application of the neospirillum deserticola CDCHPC1 in purification of inorganic nitrogen and phosphorus in a water body, and belongs to the technical field of microbial purification of mariculture water quality. The novel bacillus deserticola CDCHPC1 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC No: 64120, and the preservation time is December 06, 2023, the preservation number is CGMCC No: 64120, and the preservation number is CGMCC No: 64120. The bacterial strain CDCHPC1 disclosed by the invention is screened from gill and digestive tract tissues of cone sparus canine in sea areas of symmetry islands, has no adverse effect on cultured fishes and shrimps, has relatively high purification capacity on nitrite, nitrate and phosphate in a culture water body, and is good in environmental adaptability. The method provided by the invention meets the practical demand of purifying the seawater fish and shrimp high-density culture water environment, and has a wide application prospect in the field of water purification.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial purification of seawater aquaculture water quality, and in particular to a desert neospirilla cdchpc1 from Chenhang Island fish source and its application in purifying inorganic nitrogen and phosphorus in water. Background Art

[0002] Ammonia nitrogen and nitrite nitrogen are easily accumulated in high-density intensive aquaculture water bodies. High concentrations of ammonia nitrogen and nitrite have serious toxic effects on aquatic animals and endanger the health of farmed animals. Commonly used methods for purifying harmful nitrogen mainly include physical methods, chemical methods and biological methods. Among them, the physical method uses the adsorption function of water purification materials such as zeolite powder, dolomite powder, and coral sand to adsorb and purify pollutants in water bodies. The adsorption capacity is closely related to the physical form of the material and there are certain functional limitations. The aquaculture industry mostly uses drainage and replacement to alleviate eutrophication of water bodies. The chemical method uses the strong oxidation reaction of chemical oxidants such as quicklime and chlorine preparations to purify water quality. The purification effect continues to decrease with the consumption of oxidants. If used improperly, there is a safety risk of causing secondary pollution of water bodies. The biological method mostly uses specific microorganisms, microalgae and aquatic plants and animals to transform and utilize eutrophic substances in water bodies. Compared with physical and chemical methods, it is environmentally friendly and has a continuous and stable purification function. Some scholars use a multi-biological combination of marine microalgae + shellfish to remove excess nitrogen and phosphorus nutrients in water bodies; others use salt-tolerant plants such as alkali sedge, seahorse teeth, and mangroves to purify the marine aquaculture water environment in situ. The eutrophication level of high-density fish and shrimp intensive aquaculture water bodies is high, the water consumption is large, and the water purification efficiency requirements are relatively high. It is more appropriate to use efficient microbial water purification technology. The activated sludge method is a common microbial water purification technology. The activated sludge culture method commonly used in water treatment projects is mostly in-situ microbial community enrichment culture. Some scholars use activated sludge to enrich and culture nitrifying bacteria. At 30°C, pH 6.5-8.0, and dissolved oxygen 2.0mg / L, the total amount of nitrifying bacteria increased by nearly 20 times; the removal rate of ammonia nitrogen using enriched freshwater nitrifying bacteria is 0.12mg / g·h, and that of seawater nitrifying bacteria is 0.13mg / g·h. However, for water environment purification applications in aquaculture, the activated sludge enrichment culture method has certain shortcomings in ensuring the specificity of the dominant bacterial flora and the safety of aquaculture. In actual applications, the method of expanding the culture of purified strains is relatively rarely used on a large scale in water treatment projects due to its high requirements on the function and growth characteristics of the strains.

[0003] There are many islands and reefs and fishery organisms in the South China Sea, and the marine microbial resources are also extremely rich. It is more conducive to improving the adaptability of strains to the marine aquaculture environment, the efficiency of ecological function realization, and the safety of aquaculture organisms by digging out bacterial species resources that can be used for efficient purification of aquaculture water environments from healthy fish bodies in the South China Sea. Therefore, it is of great significance to isolate sand bacteria from marine organisms in the South China Sea and scientifically use the bacteria for water purification according to the actual needs of purifying the high-density aquaculture water environment of marine fish and shrimp. Although scholars have conducted a lot of research and exploration on the effects of Bacillus, Paracoccus, Rhodococcus, and Pseudomonas in purifying aquaculture water bodies, there are few reports on the research or application of using deep-sea fish sources in the South China Sea to purify aquaculture water bodies. Summary of the invention

[0004] The purpose of the present invention is to provide a desert neospirillum CDCHPC1 from Chenhang Island fish source and its application in purifying inorganic nitrogen and phosphorus in water to solve the problems existing in the above-mentioned prior art. The strain CDCHPC1 provided by the present invention can significantly reduce the concentration of nitrite nitrogen, nitrate and phosphate in water.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] Technical Solution 1: A desert spirillum (Caenispirillum deserti) CDCHPC1 from Chenhang Island fish source has been deposited in the Guangdong Provincial Microbiological Culture Collection Center, with the preservation number GDMCC No: 64120 and the preservation time being December 6, 2023.

[0007] The bacteria were screened from the gills and digestive tract tissues of fresh fish of healthy Pentapodus caninus in the waters of Chenhang Island in the South China Sea.

[0008] Technical solution 2: Application of the aforementioned New Spirillum deserticola CDCHPC1 in purifying water.

[0009] Furthermore, the water body includes aquaculture water.

[0010] The aquaculture water body includes seawater aquaculture pond water body.

[0011] Furthermore, the purification is to reduce the content of inorganic nitrogen and phosphorus in the water body.

[0012] Technical solution three: Application of the aforementioned Desert Neospirillum CDCHPC1 in high-density zero-water-change aquaculture of fish and shrimp.

[0013] Technical Solution 4: A bacterial agent for reducing the content of inorganic nitrogen and phosphorus in water, the bacterial agent comprising the aforementioned Desert Neospirillum CDCHPC1.

[0014] Furthermore, reducing the inorganic nitrogen and phosphorus content in the water body includes reducing the concentrations of nitrite nitrogen, nitrate and phosphate in the water body.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The strain CDCHPC1 disclosed in the present invention is screened from the gills and digestive tract tissues of the dogtooth cone-tooth sea bream in the waters of Chenhang Island, and has no adverse effects on farmed fish and shrimp; the strain CDCHPC1 has a strong ability to remove nitrite, nitrate and phosphate in fish and shrimp farming water, and has a significant effect, and the strain has good environmental adaptability and is suitable for most marine aquaculture pond water applications; the strain CDCHPC1 disclosed in the present invention is applied to water purification for intensive marine fish and shrimp farming, which can achieve good production application effects, and can greatly reduce the concentrations of nitrite nitrogen, nitrate and phosphate in water without the need to purchase and install complex water environment purification equipment, and has broad application prospects in the field of water purification. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 is the change of phosphate concentration in water under different salinities;

[0019] Figure 2 is the change of nitrite nitrogen concentration in water bodies under different salinities;

[0020] Figure 3 The changes of bacterial count of CDCHPC1 strain under different salinities;

[0021] Figure 4 is the change of phosphate concentration in water at different temperatures;

[0022] Figure 5 is the change of nitrite nitrogen salt concentration in water at different temperatures;

[0023] Figure 6 is the change of nitrate concentration in water at different temperatures;

[0024] Figure 7 The changes of bacterial count of CDCHPC1 strain at different temperatures;

[0025] Figure 8 is the change of phosphate concentration in water under different pH conditions;

[0026] Fig. 9 The change of nitrite nitrogen salt concentration in water under different pH conditions;

[0027] Fig.10 The change of nitrate concentration in water under different pH conditions;

[0028] Fig.11 The changes in the bacterial count of CDCHPC1 strain under different pH conditions. DETAILED DESCRIPTION

[0029] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0030] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0031] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0032] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0033] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0034] Example 1 Screening and cultivation of strains

[0035] 1. Strain origin and isolation and screening

[0036] A number of healthy Pentapodus caninus with a body length of 16-25 cm were collected from the waters of Chenhang Island in the South China Sea. The gills and digestive tract tissues of 4-6 fresh fish were placed in culture medium and cultured with shaking at room temperature to obtain samples and preserve them.

[0037] The sample is cultured with a photosynthetic bacteria liquid culture medium under shaking for 2-6 days at a temperature of 28-35°C; the bacterial solution is streaked with a photosynthetic bacteria solid plate culture medium, and after 3-5 days, a single colony with good growth performance is selected; strains that can remove phosphate, nitrate nitrogen and nitrite nitrogen in water are determined and sorted. The strains with good growth performance and obvious removal effects on phosphate, nitrate nitrogen and nitrite nitrogen are identified and preserved.

[0038] 2. Culture medium

[0039] Photosynthetic bacteria liquid culture medium: CH 3 COONa 3g, yeast extract 1g, peptone 5g, MgSO 4 7H 2 O 0.2g, NH 4 Cl 0.05g, NaCl 28g, NaNO 2 0.05g, KNO 3 0.18g, KH 2 PO 4 0.01g, growth factor solution 1mL, the above drugs were dissolved in distilled water, the volume was made up to 1000mL, pH 7.0.

[0040] Growth factor solution: MnSO 4 ·H 2 O 0.25g, FeSO 4 7H 2 O 7g, CaCl 2 5g, 0.02g glutamic acid, the above drugs were dissolved in distilled water, the volume was fixed to 100mL, pH 7.0.

[0041] Solid plate culture medium for photosynthetic bacteria: Add 20-25 g / L agar powder to the liquid culture medium for photosynthetic bacteria to prepare a solid plate culture medium.

[0042] Example 2 Identification of strains

[0043] The 16S rDNA molecule was identified for strains with good growth performance and obvious removal effects on phosphate, nitrate nitrogen and nitrite nitrogen. The species of the strain was determined at the molecular level, combined with the analysis of bacterial morphological characteristics and physiological and biochemical characteristics. The 16S rDNA sequence analysis mainly follows the following steps:

[0044] 1. Extraction of bacterial genomic DNA:

[0045] Pick a single colony and inoculate it in the expansion medium for cultivation; take 1.5mL of bacterial solution, centrifuge at 11500g for 1-2min, and remove the supernatant; suspend the bacterial precipitate with 200μL buffer GA, add 20mg / mL lysozyme, treat at 37℃ for 30min, add 20μL proteinase K solution; add 220μL buffer GB, treat at 70℃ for 10min, and the solution becomes clear; add 220μL anhydrous ethanol and oscillate for 15s; add the solution and flocculent precipitate to adsorption column CB3, centrifuge at 13400g for 30s, and place the adsorption column CB3 in a collection tube; add 500μL buffer GD, centrifuge at 13400g for 30s, and adsorb Column CB3 is placed in a collection tube; 700 μL of rinse solution PW is added, centrifuged at 13400g for 30s, and adsorption column CB3 is placed in a collection tube; 500 μL of rinse solution PW is added, centrifuged at 13400g for 30s, and adsorption column CB3 is placed in a collection tube; centrifuged at 13400g for 2min, waste liquid is removed, and adsorption column CB3 is placed at room temperature for 5min to dry; adsorption column CB is placed in a clean centrifuge tube, 50-200 μL of elution buffer TE is added, placed at room temperature for 3-5min, centrifuged at 13400g for 2min, and the solution is collected in a centrifuge tube; the concentration and purity of the obtained DNA fragments are detected by agarose gel electrophoresis and UV spectrophotometer.

[0046] 2. PCR amplification of 16S rDNA gene

[0047] The bacterial universal primers for amplification of 16S rDNA were synthesized by Sangon Biotech (Shanghai) Co., Ltd. The forward primer 8F was: 5'-AGAGTTTGATCCTGGCTCAG-3' (SEQ ID NO.1); the reverse primer 1492R was: 5'-GGTTACCTTGTTACGACTT-3' (SEQ ID NO.2);

[0048] The reaction conditions for PCR amplification were as follows: 95°C for 3 min, 95°C for 1 min, 48°C for 1 min, and 72°C for 2 min, for a total of 30 cycles; 72°C for 10 min;

[0049] The reaction system for PCR amplification: 37 μL of sterile double distilled water, 1 μL of each primer, dNTPs (2.5 mmol / L), 1 μL of Tap enzyme, 5 μL of 10×PCR buffer, and 1 μL of DNA template.

[0050] 3.16S rDNA sequence determination

[0051] The PCR products were detected by 1.0% agarose gel electrophoresis and sequenced by Sangon Biotechnology (Shanghai) Co., Ltd. The sequence was as follows:

[0052] CGTCAGATTAGGTAGTTGGTGAGGTAACGCTCACCCCGCCGACGATCCGTAACTG

[0053] GTCTGAGAGGCTGATCAGCCACGCTGGGACTGATACCCGGCCCAGACTCCTACGGGAG

[0054] GCAGCTGTGGGGAATATTGGACCATGGGCGCAAGCCTGATCCACCCATGCCGCGCGACC

[0055] GACAAGGCCTTCGGGTTGTAAAGCTCTTTCCCAAGTGACAATGATGACTGTAACTTCT

[0056] GAAGAACCCTCGGCTAACTTCATGCCATCAGCCGCGGCAATACGAAGGGGGCAAGCGT

[0057] TGTTCGCAATTACTGGGCGTAAAGAGCGCGTACGCGGTGTGCATAGTCAGGGGTGAAAT

[0058] CCCGGGGCTCAACCTCGGAATTGCCTTTGATACTAGCACGCTATAATCCGTGATAAGGTG

[0059] GTGGAATGACCAGTGTACATGTGAAATTTTTAGATATTGGTCGGAACACCTGTGGCGAA

[0060] GGCGGCGCCTGGCGCGGTATTGACGCTGAGGCGCGAAGACGTGGAGAGCAACCAGGA

[0061] TTAGATACCCTGGTAGGCACGCCGTAAACCCATGAGTGCTAGCTGTTGGCGTGCTTGCC

[0062] GGTCATACCTTTCCGTGGCCCGACTGCCTCCGTTGCCGGTTGGCGCATCGTCTTCGGGTA

[0063] AAGCCAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGT

[0064] GGCATGCTGATCCACGATTACTAGCGATTCCGACTTCATGCACTCGAGTTGCAGAGTGCA

[0065] ATCCGAACTGAGACATCTTTTGGAGATTAGCTCCACGTCGCCGTGTGGCTGCCCATTGTA

[0066] GATGCCATTGTAGCACGTGTGTAGCCCAGCCCGTAAGGGCCATGAGGACTTGACGTCAT

[0067] CCCCACCTTCCTCCGACTTGTCATCGGCAGTTCCCCTAGAGTGCCCAACTGAATGCTGGC

[0068] AACTAAGGGCGAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAG

[0069] CTGACGACAGCCATGCAGCACCTGTGCGGGATCCAGCCGAACTGAAAGTCTCATCTCTG

[0070] AGACCGCGATCCCCATGTCAAGGGCTGGTAAGGTTCTGCGCGTTGCTTCAAATTAAACC

[0071] ACATGCTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTTAACCTTGCGGCCG

[0072] TACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCGCCACTGACCTGCAAAGCAGGCCA

[0073] ACAGCTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCC

[0074] CACGCTTTCGCGCCTCAGCGTCAATACCGCGCCAGGTGGCCGCCTTCGCCACTGGTGTT

[0075] CCGACCAATATCTACGAATTTCACCTCTACACTGGTCATTCCATCACCCTCTCTCACGGAT

[0076] TCTAGCGTGCTAGTATCAAAGGCAATTCCGAGGTTGAGCCCCCGGGATTTTCACCCCTGA

[0077] CTATGCACACCGGCCTACTCCGCTTTACGCCCATAATTT (SEQ ID NO. 3).

[0078] 4. Colony morphology and physiological characteristics of strains

[0079] The colony morphology and physiological characteristics are shown in Table 1.

[0080] Table 1 Colony morphology and physiological characteristics

[0081]

[0082] 5. Identification of strains

[0083] Based on 16S rDNA gene sequence analysis, biochemical identification, and morphological characteristics, the strain was identified as Caenispirillum deserti. After consulting the information, there are no reports on the use of Caenispirillum deserti collected from fish in the waters of Chenhang Island to purify inorganic nitrogen and phosphorus in intensive aquaculture waters of fish and shrimp. The strain was named Caenispirillum deserti CDCHPC1. The Caenispirillum deserti CDCHPC1 was deposited in the Guangdong Provincial Microbial Culture Collection Center on December 6, 2023, with the deposit number GDMCC No: 64120, and the deposit address is: 5th Floor, Building 59, No. 100 Xianlie Middle Road, Guangzhou.

[0084] Example 3 Application of Neospirillum deserticola CDCHPC1

[0085] 1. Removal effect of Neospirillum deserticola CDCHPC1 on inorganic nitrogen and phosphorus in water at different salinities

[0086] The sterilized marine fish and shrimp intensive breeding pond water (water salinity 25) was used as the basic test water control, and the strain CDCHPC1 was not added. The bacterial addition group used distilled water and sea salt to adjust the water salinity to 5, 10, 25 and 40, and the strain CDCHPC1 identified in Example 2 was added at 10 5 -10 6Each group of test samples was set up with 3 parallels. The changes in the concentrations of phosphate and nitrite nitrogen in the water were monitored every 3 days.

[0087] like Figure 1 As shown, the phosphate concentration in the control group did not change much compared with the initial value, maintaining at 8.273-10.135 mg / L; the phosphate removal effect of the bacteria-added group with salinity of 5-40 was obvious. On the third day, the concentration values ​​decreased from the initial 9.928-10.342 mg / L to 0.967-3.792 mg / L, and the removal rate reached 61.8%-90.6%. However, on the sixth day, the phosphate concentrations in the control group and each bacteria-added salinity group increased to varying degrees.

[0088] like Figure 2 As shown, the nitrite nitrogen concentration in the control group was maintained at 7.562-9.242 mg / L; the nitrite nitrogen removal effect of the bacteria-added group with salinity of 25 and 40 was obvious and stable. On the 3rd to 6th days, their concentration values ​​were all lowered to 4.016-4.649 mg / L, and the removal rate was 44.3%-54.8%.

[0089] like Figure 3 As shown in Figure 2, under the condition of salinity 5-40, the bacterial count in the water of each bacterium-added salinity group continued to increase during the test period, with the range of the number change being 4.0×10 5 / mL-3.9×10 8 Pieces / mL.

[0090] It can be seen that strain CDCHPC1 has good adaptability to salinity and can grow normally at salinity of 5-40. However, it can only achieve the effect of removing nitrite nitrogen in a seawater environment with a salinity greater than 25; generally, the comprehensive effect of nitrogen and phosphorus removal in the water body is better on the third day after adding bacteria. When using it, attention should be paid to controlling the effect time of bacteria, or repeating the use of bacteria on the third day to strengthen or stabilize the purification effect.

[0091] 2. Removal effect of Neospirillum deserticola CDCHPC1 on inorganic nitrogen and phosphorus in water at different temperatures

[0092] Sterilized marine fish and shrimp intensive breeding pond water (water salinity 25, pH 8.0) was used as the basic test water control and cultured at 30°C without adding strain CDCHPC1. 5 -10 6The samples were inoculated into test water bodies at different temperatures. The incubation temperature gradient was set at 10°C, 20°C, 30°C and 40°C. The constant temperature incubation test lasted for 6 days. Three parallels were set for each group of test samples. The changes in the concentrations of phosphate, nitrite nitrogen and nitrate nitrogen in the water bodies were monitored every 3 days.

[0093] like Figure 4 As shown, the phosphate concentration of the control group did not change much compared with the initial value, maintaining at 10.097-11.228 mg / L; at 10-30°C, the strain CDCHPC1 had a good phosphate removal effect, with a removal rate of 71.2%-87.6% in 6 days; the removal rate of the 40°C group was 53.2% on the 3rd day, and decreased to 18.7% on the 6th day.

[0094] like Figure 5 As shown, the nitrite nitrogen concentrations in the control group, 10℃ group and 20℃ group did not change much compared with the initial value, and remained at 7.328-9.523 mg / L; at 30-40℃, the strain CDCHPC1 had a good removal effect on nitrite nitrogen, and the removal rate in 3-6 days could reach 98.0%-99.6%.

[0095] like Figure 6 As shown, the nitrate nitrogen concentrations in the control group, 10℃ group and 20℃ group did not change much compared with the initial value, and remained at 33.253-37.060 mg / L; at 30-40℃, the strain CDCHPC1 had a good removal effect on nitrate nitrogen, and the removal rate on the 6th day could reach 72.2%-98.4%.

[0096] like Figure 7 As shown in the figure, the bacterial count in the water of each temperature group continued to increase during the test period under the temperature of 10-40℃. On the 6th day, the bacterial count values ​​between the temperature groups were not much different, with a range of 2.4×10 8 / mL-5.7×10 8 / mL, all stable at 10 8 The level is on the order of magnitude of cells / mL.

[0097] It can be seen that strain CDCHPC1 has good temperature adaptability and can grow normally under 10-40℃ conditions. However, considering its purification effect on phosphate, nitrite nitrogen and nitrate nitrogen in the water environment, a temperature of 25-30℃ is more suitable, which also coincides with the suitable water temperature conditions for most aquatic animals.

[0098] 3. Removal effect of desert neospirilla cdchpc1 on inorganic nitrogen and phosphorus in water at different pH

[0099] Sterilized marine fish and shrimp intensive breeding pond water (water salinity 25, pH 8.0) was used as the basic test water control and cultured at 30°C without adding strain CDCHPC1. 5 -10 6 CFU / mL was inoculated into test water bodies with different pH values, and the pH values ​​were set to 6, 8, and 10, respectively. The culture was kept at 30°C for 6 days, and 3 parallels were set for each group of test samples. The concentration changes of phosphate, nitrite nitrogen, and nitrate nitrogen in the water bodies were monitored every 3 days.

[0100] like Figure 8 As shown, the phosphate concentration of the control group did not change much compared with the initial value, and remained at 12.997-14.661 mg / L. The strain CDCHPC1 had a good removal effect on phosphate at pH 6-pH 10. Relatively speaking, the effect was better under weak alkaline conditions. The phosphate removal rate of the pH 8-10 group was 81.4%-93.7% at 3-6 days, and the pH 6 group was slightly lower, at 70.4%-82.6%.

[0101] like Fig. 9 As shown, the nitrite nitrogen in the control group did not change much compared with the initial value, and remained at 8.872-9.179 mg / L. The strain CDCHPC1 had a good removal effect on nitrite nitrogen at pH 6-pH 10. The effect was better under weak alkaline conditions. The nitrite nitrogen removal rate of the pH 8 group was 95.7%-96.8% at 3-6 days, and the pH 6 group and pH 10 group were slightly lower, at 71.0%-82.7% and 77.0%-86.1%, respectively.

[0102] like Fig.10 As shown, the nitrate nitrogen in the control group did not change much compared with the initial value, and remained at 11.579-13.490 mg / L. The strain CDCHPC1 had a good removal effect on nitrate nitrogen at pH 6-10. The nitrate nitrogen removal rates of the pH 6 group, pH 8 group, and pH 10 group at 3-6 days were 55.3%-87.3%, 62.4%-88.8%, and 68.8%-69.5%, respectively.

[0103] like Fig.11 As shown in Figure 2, the bacterial counts in each group continued to increase under the conditions of pH 6-pH 10. On the 6th day, the bacterial counts in each pH group were not much different, and the number was stable at 10 8 The level is on the order of magnitude of cells / mL.

[0104] It can be seen that strain CDCHPC1 can adapt to most of the pH environments of marine aquaculture water bodies, and can survive and grow well in water bodies with a pH range of 6-pH10, reaching 10 8CFU / mL quantity level, and it has a good effect on removing phosphate, nitrite nitrogen and nitrate nitrogen in water bodies.

[0105] Example 4 Application effect of Neospirillum deserticola CDCHPC1 in high-density zero-water-change aquaculture of fish and shrimp

[0106] The strain CDCHPC1 was tested in high-density shrimp aquaculture with zero water exchange in Jiaxi Town, Shanwei, Guangdong and Dianbai, Maoming, Guangdong. The strain CDCHPC1 was added within 10 days of seeding (with a concentration of 10 4 -10 6 CFU / mL) and brown sugar (0.05kg / m 3 ) to the aquaculture water, and then add bacterial agents regularly every 6 days, use quicklime water and other methods to stabilize the total alkalinity and pH value of the water, and use aeration machinery to increase the dissolved oxygen in the water. The results showed that the application of bacterial agents had a good effect, and the strain CDCHPC1 had no adverse effects on farmed fish and shrimp; the Dianbai breeding base passed the on-site test organized by the expert group of Maoming Agricultural Technology Extension Center. The shrimp was cultured for 90 days and the tilapia was cultured for 120 days. The average weight of the shrimp was 14.7g / tail, the body length was 12.1cm, and the estimated yield was 10kg / m 3 , survival rate 82%; average fish weight 826g / tail, estimated yield 23kg / m 3 , survival rate 70%. The water conditions during the breeding process are shown in Table 2, water temperature 23-28 ° C, salinity 10-15, pH value 7.0-8.5, dissolved oxygen concentration 5.02-6.50 mg / L, ammonia nitrogen 0.145 mg / L and nitrite nitrogen 0.119 mg / L.

[0107] Table 2 Water quality of aquaculture water using strain CDCHPC1

[0108]

[0109] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A Caenispirillum deserti CDCHPC1 from Chenhang Island fish source, characterized in that: It has been deposited in Guangdong Provincial Microbiological Culture Collection Center, with the deposit number GDMCC No: 64120 and the deposit time being December 6, 2023.

2. Application of the desert neospirilla CDCHPC1 according to claim 1 in purifying water.

3. The use according to claim 2, characterized in that: The water body includes aquaculture water body.

4. The use according to claim 2, characterized in that: The purification is to purify the inorganic nitrogen and phosphorus in the water body.

5. Application of the Desert Neospirillum CDCHPC1 described in claim 1 in high-density zero-water-change aquaculture of fish and shrimp.

6. A bacterial agent for reducing the content of inorganic nitrogen and phosphorus in water, characterized in that: The bacterial agent includes the desert neospirillum CDCHPC1 described in claim 1.

7. The product according to claim 6, characterized in that The reducing the inorganic nitrogen and phosphorus content in the water body includes reducing the concentrations of nitrite nitrogen, nitrate and phosphate in the water body.

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