Preparation method and application of chitosanase NFCSN
By codon optimization and signal peptide removal of chitosanase gene, the highly hydrolyzed chitosanase NFCSN was expressed in Pichia yeast, which solved the problem of low hydrolyzed chitosanase in the prior art, and achieved efficient preparation of chitosanase and good industrial application prospects.
CN119979507APending Publication Date: 2025-05-13湖州检验检疫综合技术服务中心
View PDF 0 Cites 0 Cited by
Patent Information
- Application Number
- CN202510073350.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2025-05-13
Smart Images
Figure CN119979507A_ABST
Abstract
The invention discloses a preparation method and an application of chitosanase NFCSN. According to the present invention, a potential chitosanase gene derived from Neosatora fischeri is subjected to secretory expression in pichia pastoris, such that the potential chitosanase gene derived from Neosatora fischeri can be obtained, the chitosanase obtained by the invention has higher hydrolytic activity to a chitosan substrate, a crude enzyme liquid generated by shake flask fermentation has the capability of degrading 5g of chitosan by 1mL (the content of crude protein is about 0.23 mg), while about 150mg of non-specific commercial neutral protease is needed for hydrolyzing the same amount of chitosan, the enzymolysis efficiency is improved by more than 600 times, and the enzymolysis time is shortened by more than 30 minutes. The industrial application potential of large-scale preparation of chitosan oligosaccharide is realized.
Need to check novelty before this filing date? Find Prior Art