Lightweight platelet function detection device and method

Abstract

The invention discloses a lightweight platelet function detection device. The lightweight platelet function detection device comprises a detection module, a sample processing module, a numerical control module and a data processing module, the sample treatment module comprises a multifunctional liquid suction needle for sucking liquid, a driving device and a dilution module; the multifunctional liquid suction needle is connected with the dilution module through the liquid path pipe, and the driving device controls the multifunctional liquid suction needle to move. The detection module comprises one or more sample cup positions for placing sample cups, and one or more built-in reagent positions are arranged in the device. All the sample cup positions and the built-in reagent positions are arranged in a movement track area of the multifunctional liquid suction needle; the numerical control module controls the sample processing module and the detection module to operate, and the data processing module processes detection data. According to the design, the structure and the flow of the platelet function detection device adopting the original continuous counting method are simplified, and electromagnetic valves required by the device are reduced by 30%, so that the cost of the device is reduced, and the failure rate is correspondingly reduced.

Description

Technical Field

[0001] The invention relates to the technical field of medical devices, and in particular to a lightweight platelet function detection device and method. Background Art

[0002] This section merely provides background information related to the present disclosure and is not necessarily prior art.

[0003] Platelet function testing instruments play a very important role in early warning and prevention of thrombotic diseases, and in guiding the safe, effective and precise use of antiplatelet drugs in the prevention and treatment of thrombotic diseases. In addition, they also play a very important role in other clinical platelet function-related tests and related drug research.

[0004] The most ideal sample for platelet function testing is citrate anticoagulated whole blood sample, but the platelet function in citrate anticoagulated whole blood sample is more susceptible to changes due to factors such as environmental temperature, shock, and time. Therefore, it is required that platelet function testing of blood samples must be completed quickly in clinical medical services and related research. For whole blood sample testing, it is also necessary to always keep the number and volume of platelets (including platelets) in the blood sample in a mixed state to obtain correct results.

[0005] In order to explore the ideal platelet function test instrument method, scholars have developed a variety of platelet function meters with different principles and methods. Among them, the continuous counting platelet function test method has better test result quality. However, the instrument requires more than one diluter to realize the operation of the instrument, and the sample mixing device of the instrument has certain deficiencies in mixing viscous blood samples with reagents, resulting in insufficient reaction between some samples and reagents. In order to simplify the complex structure of the original continuous counting platelet function meter, improve the mixing effect of blood samples and reagents, and better improve the quality of platelet function test results, the original continuous counting platelet function meter has been improved. However, the original design of the instrument has a complex structure and high cost, and the mixing of blood samples and reagents is somewhat insufficient. Summary of the invention

[0006] In view of the above-mentioned deficiencies in the prior art, the present invention discloses a lightweight platelet function detection device and method.

[0007] A lightweight platelet function detection device includes a detection module, a sample processing module and a detection module. The sample processing module includes a multifunctional liquid suction needle for sucking liquid, a driving device and a dilution module for diluting samples; the multifunctional liquid suction needle is connected to the dilution module through a liquid line tube, and the driving device controls the movement of the multifunctional liquid suction needle. The multifunctional liquid suction needle realizes quantitative absorption and distribution of reagents, and has the functions of quantitatively sucking samples and diluting samples and performing distribution according to detection needs; the multifunctional liquid suction needle cooperates with the dilution module to realize quantitative addition of diluent into the detection cup. The dilution module is connected to the dilution liquid barrel through a pipeline.

[0008] The detection module is used to detect the cell components of the blood sample and the components contained in the plasma. The detection module includes a sample cup position for placing the sample cup and a built-in reagent position for placing the detection reagent. The built-in reagent positions are all located on the movement track of the multifunctional liquid pipetting needle.

[0009] The device comprises a numerical control module and a data processing module, wherein the numerical control module controls the operation of the sample processing module and the detection module, and the data processing module processes the detection data.

[0010] The multifunctional pipette needle is capable of moving in more than one dimension. The multifunctional pipette needle can be inserted into the sample cup to absorb the mixed blood sample and distribute it to the dilution cup, and a certain amount of diluent is added to the dilution cup. After the blood sample is diluted and mixed in the dilution cup, the multifunctional pipette needle again absorbs a certain amount of diluted blood sample from the dilution cup and distributes it to the detection cup for detection. The device is also equipped with a dilution liquid barrel, which can be external or internal; the sample cup matched with the device can be an independent single cup, or a combined cup composed of multiple single cups with the same structural shape.

[0011] Furthermore, the built-in reagent position includes a built-in reagent position for placing a dilution cup, a detection position for placing a detection cup, and a detection position for placing a reagent bottle. The reagent bottle is used to place an aggregation inducer. The dilution cup and the sample cup are respectively provided with a stirring device. The lower part of the dilution cup and the detection cup is provided with a drain port, and the dilution cup and the detection cup are connected to the waste liquid treatment module through the drain port.

[0012] The waste liquid treatment module includes a third liquid flow control valve and a first liquid discharge pump. The dilution cup and the detection cup are both connected to the third liquid flow control valve through a waste liquid discharge pipe. The third liquid flow control valve is connected to the first liquid discharge pump through a waste liquid discharge collection pipe.

[0013] Furthermore, in some embodiments, the stirring device includes a magnetic stirring device and a magnetic stirring bar, the magnetic stirring bar is arranged in the detection cup, and the magnetic stirring device is arranged at the outer bottom of the detection cup, and the magnetic stirring device drives the magnetic stirring bar to move through magnetic force to stir the sample.

[0014] The bottom of the dilution cup and the detection cup are respectively provided with radial shallow grooves, which are adapted to the shape of the magnetic stirrer. The magnetic stirrer falls into the radial shallow grooves after the stirring stops, and the magnetic stirrer is in a stable static state when not stirring, so as to avoid interference of the stirring bar movement on the detection during the device detection.

[0015] Furthermore, in some embodiments, the stirring device is an inflation mixing needle arranged in the dilution cup and the detection cup and an air supply pump connected to the inflation mixing needle, the exhaust port of the inflation mixing needle is respectively located in the dilution cup and the detection cup, and the air supply pump provides disturbance gas to the inflation mixing needle to stir and mix the liquid in the dilution cup and the detection cup.

[0016] Furthermore, the dilution module includes a diluter and a diluent bucket for placing diluent; the multifunctional liquid pipette, the first liquid line tube, the diluter, the second liquid line tube and the diluent bucket are connected in sequence, the first liquid line tube is provided with a first liquid flow control valve, and the second liquid line tube is provided with a second liquid flow control valve. The system performs multiple functions such as blood sample aspiration, reagent aspiration and distribution, and directly quantitatively distributes diluent to the dilution cup and the detection cup to achieve blood sample aspiration, distribution, dilution, and also has multiple functions such as adding platelet function detection reagents to the sample cup.

[0017] When it is necessary to add diluent to the detection cup through the multifunctional liquid pipette needle, the first liquid flow control valve is closed, the second liquid flow control valve is opened, the piston of the diluter is retracted, and a negative pressure is formed in the second liquid circuit pipe so that the diluent in the diluent barrel is sucked into the diluent cavity; then the second liquid flow control valve is closed, the first liquid flow control valve is opened, the piston of the diluter is pushed forward, and the diluent sucked in the diluent cavity is passed through the first liquid circuit pipe to the multifunctional liquid pipette needle and distributed into the detection cup.

[0018] Furthermore, the detection cup and the reagent bottle are respectively provided with automatic opening and closing covers, which are opened and closed in coordination with the movement of the multifunctional liquid-absorbing needle. When the multifunctional liquid-absorbing needle needs to go down into the reagent bottle to absorb the reagent, or needs to add the sample or reagent to the detection cup, the automatic opening and closing cover automatically opens, and when the multifunctional liquid-absorbing needle completes absorbing and mixing the sample from the detection cup, or completes adding the reagent / diluent to the detection cup, and completes the reagent absorption, the automatic opening and closing cover automatically closes to prevent the liquid absorbed by the multifunctional liquid-absorbing needle from dripping into the detection cup or reagent bottle at the bottom. The automatic opening and closing cover also has the function of preventing the reagent carried by the multifunctional liquid-absorbing needle and other related devices from dripping into the detection cup and the reagent bottle.

[0019] The material of the automatic switch cover is a material that resists external electrical signal interference. When the detection cup performs detection, the interference of external electromagnetic signals on the device detection signal can be avoided and reduced, thereby improving the quality of the device detection result.

[0020] Furthermore, the platelet function testing device is equipped with a special cleaning agent.

[0021] In some embodiments, the lightweight platelet function detection device performs detection without adding platelet function aggregating agents and platelet function inhibitors to the sample in advance, and the workflow includes the following steps:

[0022] Step 1, pre-preparation, add the loaded blood sample to be tested and the magnetic stirrer into the sample cup, place the sample cup on the sample cup position, start the device, and the device controls the stirring device through the CNC module to mix the sample in the sample cup.

[0023] The third liquid flow control valve at the bottom of the dilution cup and the detection cup is opened, and the first liquid discharge pump works to discharge the dilution liquid originally in the dilution cup and the detection cup. The multifunctional liquid aspiration needle cooperates with the diluter to add a certain amount of dilution liquid to the dilution cup and the detection cup respectively, and the dilution cup and the detection cup are rinsed through the stirring device. After rinsing, the rinsed dilution liquid is discharged.

[0024] The multifunctional liquid aspirating needle cooperates with the diluter to add a certain amount of diluent into the release cup and the detection cup respectively. At this time, the diluent added into the two cups is kept in the cups.

[0025] Step 2, dilute the sample, move the multifunctional pipette needle to one of the sample cups, cooperate with the diluter to absorb a certain amount of blood sample and transfer it to the dilution cup, add a certain amount of diluent to the dilution cup, and mix the blood sample in the dilution cup through the stirring device.

[0026] Step 3, original detection, the multifunctional pipette needle draws a certain amount of diluted blood sample from the dilution cup and transfers it to the detection cup, and adds a certain amount of diluent to the detection cup, and mixes the blood sample in the detection cup through the stirring device. The detection cup performs detection on the blood sample in the cup through the detection device to obtain the information data of the original state of platelets in the blood sample.

[0027] Step 4, waste liquid treatment, the drainage channel at the bottom of the dilution cup and the detection cup is opened, and the waste liquid in the dilution cup and the detection cup is discharged through the connecting pipe and the first drainage pump.

[0028] Step 5, cleaning device, adding a certain amount of diluent to the dilution cup and the detection cup respectively through the multifunctional liquid pipette needle and the diluter, and rinsing the dilution cup and the detection cup through the stirring device, and discharging the rinsing diluent after the rinsing is completed.

[0029] Step 6: Use the multifunctional liquid pipette needle in conjunction with the diluter to add a certain amount of diluent into the release cup and the detection cup respectively.

[0030] Step 7, reaction detection, a certain amount of platelet aggregation agent is drawn from the reagent bottle through the multifunctional pipette needle and added to the sample cup for completing the original blood sample test, the blood sample and the reagent in the sample cup are stirred and mixed by the stirring device, and after the reaction time is sufficient, the multifunctional pipette needle draws the reacted blood sample from the sample cup and performs the test according to the above steps 1-5 to obtain the number, volume and other information data of the platelets after the aggregation reaction in the blood sample treated with the aggregation agent.

[0031] Step 8, data processing, the device compares the platelet number and volume changes of the blood sample obtained by the above process before and after the addition of the aggregation agent to obtain the platelet function level test result of the blood sample;

[0032] Step 9, repeat the test, the device repeatedly executes the platelet function test results of different blood samples according to the above steps 1-8.

[0033] When the platelet function detection device detects more than one blood sample at the same time, the platelet quantity detection of each different original blood sample can be arranged at an optimized time, and the time sequence of adding the aggregation agent can be optimized to achieve optimized process detection of more than one sample, or the detection of each different sample can be completed one by one according to the above steps to obtain different samples, or different platelet receptor function test results for the same sample.

[0034] In some embodiments, the platelet function detection device can be used to pre-divide the same blood sample to be tested into more than one portion, and a strong platelet function inhibitory reagent such as EDTA is added to one portion, and different platelet receptor function inducers can be added to the remaining blood samples. Under such conditions, the device is not loaded with a built-in reagent position for the inducer, and there are more than one sample cup position, and the same blood sample is loaded in multiple sample cups each time, but different reagents are pre-added to each sample cup, and a magnetic stirrer is pre-added to each sample cup. The device directly draws blood samples from each sample cup for detection, and calculates the platelet function of each blood sample based on the platelet count or volume obtained from the detection of each cup of blood sample. The working process of the device under this condition is:

[0035] Step 1, preliminary preparation, place at least two sample cups containing equal amounts of the same blood sample in the sample cup position, and add a magnetic stirrer to each sample cup, and add different reagents to each sample cup for treatment, add a platelet function inhibitor to one of the sample cups, and add a platelet receptor function inducer to the remaining blood samples, and mix each sample by controlling the stirring device through the CNC module.

[0036] The third liquid flow control valve at the bottom of the dilution cup and the detection cup is opened, and the first liquid discharge pump works to discharge the dilution liquid originally in the dilution cup and the detection cup; the multifunctional liquid pipette needle cooperates with the diluter to add a certain amount of dilution liquid to the dilution cup and the detection cup respectively, and the dilution cup and the detection cup are rinsed through the stirring device, and the rinsed dilution liquid is discharged after the rinsing is completed.

[0037] The multifunctional liquid aspirating needle cooperates with the diluter to add a certain amount of diluent into the release cup and the detection cup respectively, and both cups retain the diluent.

[0038] Step 2, dilute the sample, move the functional pipette needle to a sample cup, and use the multifunctional pipette needle and the diluter to absorb a certain amount of blood sample and transfer it to the dilution cup, add a certain amount of diluent to the dilution cup, and mix the blood sample in the dilution cup by the stirring device.

[0039] Step 3, sample testing, the multifunctional pipette needle draws a certain amount of diluted blood sample from the dilution cup and transfers it to the test cup, and adds a certain amount of diluent to the test cup, and the blood sample in the test cup is diluted and mixed again by the stirring device. The test cup performs testing on the mixed diluted blood sample in the test cup through the detection device to obtain the platelet count and volume information of the blood sample in the blood sample cup.

[0040] Step 4, waste liquid treatment, the drainage channel at the bottom of the dilution cup and the detection cup is opened, and the waste liquid in the dilution cup and the detection cup is discharged through the connecting pipe and the first drainage pump.

[0041] Step 5, cleaning the device, adding a certain amount of diluent into the dilution cup (6) and the detection cup (7) respectively through the cooperation of the multifunctional liquid pipetting needle (2) and the diluter (11), and stirring and rinsing the dilution cup (6) and the detection cup (7) through the stirring device, and after rinsing, discharging the rinsed dilution liquid in the dilution cup (6) and the detection cup (7).

[0042] Step 6: Use the multifunctional liquid pipette needle in conjunction with the diluter to add a certain amount of diluent into the release cup and the detection cup respectively. At this time, the diluent added into the two cups is kept in the cups.

[0043] Step 7, repeat steps 2-6 to test the blood samples in the remaining sample cups, and obtain information on the status of platelets in the blood samples after the same blood samples are treated with different reagents.

[0044] Step 8, based on the difference in the test results of the same blood sample with the addition of platelet function aggregating agent and platelet function inhibitor, the platelet function level test results of the blood sample are obtained by comparison. The basis is that the citrate anticoagulated blood sample has the function of platelet activation, and the platelet function activation aggregation decreases after the platelet function aggregating agent is added to the blood sample, and the degree of reduction is related to the platelet function level; and when a strong anticoagulant, such as EDTA, is added to the citrate blood sample, the platelet function is completely inhibited. The number of platelets obtained by the EDTA blood sample test is used as the original platelet number of the blood sample. The platelet function test results of the blood sample can be obtained by adding a strong platelet function inhibitor to the same blood sample and comparing the number of platelets detected after the addition of the aggregating agent.

[0045] Step 9, repeat steps 1-8 to test the platelet function of different blood samples. The blood samples tested according to the above steps should be tested within 30 minutes at the latest after adding the reagent.

[0046] Furthermore, the platelet function detection device is provided with at least one built-in reagent position for cleaning agent, and cleaning is performed when cleaning conditions are met, the cleaning conditions including before starting the detection every day, after completing the detection, the device does not perform the detection for more than a specified time, and other set conditions. The cleaning steps include:

[0047] Step S1, a multifunctional liquid-sucking needle draws a certain amount of cleaning agent from the cleaning agent bottle and discharges it into a dilution cup and a detection cup respectively, and the cleaning agent is soaked in the dilution cup and the detection cup for a certain period of time.

[0048] Step S2, stirring the cleaning agent in the dilution cup and the detection cup by a stirring device.

[0049] Step S3, the third liquid flow control valve at the bottom of the dilution cup and the detection cup is opened, and the first liquid discharge pump is operated to discharge the cleaning agent in the dilution cup and the detection cup.

[0050] Step S4, repeating steps S1-S3 until the cleaning requirements are met.

[0051] Furthermore, the sample cup and built-in reagent positions on the platelet function detection device are partially or completely provided with a constant temperature control device. The sample cup is generally set at a temperature of 24-35°C, and the temperature of the aggregation agent built-in reagent position is generally set at a range of 5-20°C. The diluent injection pipeline can be set at a temperature of 15-25°C.

[0052] Furthermore, the sample cup of the platelet function detection device is a single-row combination of sample cups formed by combining at least two cups of the same structure, and each cup is independent of each other and not connected to each other; the height of the combination sample cup is 12-55mm, and the inner diameter of a single cup is 5-15mm.

[0053] The platelet function detection device calculates the platelet function of the two working processes based on the platelet count test results of the blood sample:

[0054] Comparison of platelet count changes before and after adding aggregating agents to blood samples:

[0055] The original blood sample without adding the platelet aggregator has a large number of platelets, while the platelets in the blood sample with high platelet function after adding the aggregator will aggregate to a higher degree, resulting in a decrease in the number of platelets in the blood sample. The platelet aggregation degree of the blood sample with low platelet function is low, and the corresponding decrease in the number of platelets is small. The platelet number obtained by the comparison device for the blood sample before and after adding the aggregator, that is, the degree of thrombocytopenia, can be used to calculate the platelet function of the blood sample.

[0056] Comparison of platelet counts obtained by adding different reagents to the same blood sample:

[0057] In this test method, the same blood sample is loaded into different sample cups, wherein a platelet function inhibitor or a strong anticoagulant such as EDTA is added to one sample cup, and a platelet function aggregating agent such as arachidonic acid, adenosine diphosphate, etc. is added to other sample cups. Since the platelet function is inactivated and no longer aggregates after the strong anticoagulant is added to the blood sample, and the platelets in the blood sample with high platelet function will aggregate to a higher degree after the platelet function aggregating agent is added to the blood sample, the platelet count in the blood sample will be greatly reduced, but when the platelet function in the blood sample is low, the platelet count will only aggregate to a low degree after the aggregating agent is added, and the degree of platelet count reduction is extremely low or low. Therefore, the platelet function test result of the blood sample can be obtained by comparing the changes in the platelet count after different types of reagents are added to the blood sample.

[0058] Beneficial effects:

[0059] The lightweight platelet function detection device and method designed by the present invention greatly simplifies the original platelet function detection device of the continuous counting method, reduces the number of diluters in the device, and reduces the number of electromagnetic valves used by 40%, thereby reducing the device cost and the failure rate accordingly;

[0060] At the same time, a device workflow adapted to the simplified device structure is designed so that the simplified device can complete platelet function testing;

[0061] The platelet function detection device of the present invention has a simple structure and can significantly reduce the cost, which is conducive to the popularization of platelet function detection services to primary medical service institutions.

[0062] In the past, similar platelet function testing devices required special consumption of diluent to clean the sample suction needle. However, the platelet function testing device of this design uses the diluent for needle cleaning, which can greatly reduce the reagent consumption of the device and is more conducive to reducing the operating cost of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0063] The present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments, and the above and / or other advantages of the present invention will become more clear.

[0064] Figure 1 This is a schematic diagram of the main structural relationships of the designed device.

[0065] Figure 2 This is a schematic diagram of the relevant structure when the designed device is equipped with two diluters. DETAILED DESCRIPTION

[0066] The accompanying drawings are marked as follows: 101-first sample cup; 102-second sample cup; 2-multifunctional pipette needle; 3-fixed drive device; 4-movable slide rail; 5-first liquid circuit tube; 6-dilution cup; 7-detection cup; 801-first reagent bottle; 802-second reagent bottle; 9-second liquid circuit tube; 10-dilution liquid barrel; 11-diluter; 12-detection device; 13-first liquid discharge pump; 14-magnetic stirring device; 15-magnetic stirring bar; 16-waste liquid discharge pipe; 17-waste liquid discharge collection pipe; 18-first liquid flow control valve; 19-second liquid flow control valve; 20-third liquid flow control valve; 21-second liquid discharge pump; 22-fourth liquid flow control valve; 23-second diluent; 24-fifth liquid flow control valve; 25-needle surface cleaning device; 26-sixth liquid flow control valve.

[0067] like Figure 1 As shown, a lightweight platelet function detection device includes a detection module, a sample processing module, a numerical control module and a data processing module. The sample processing module includes a multifunctional liquid-absorbing needle 2 for absorbing liquid, a driving device and a dilution module for diluting the sample; the multifunctional liquid-absorbing needle 2 is connected to the dilution module through a liquid line tube, and the driving device controls the movement of the multifunctional liquid-absorbing needle 2. The multifunctional liquid-absorbing needle 2 realizes quantitative absorption and distribution of reagents, and at the same time has the functions of quantitatively absorbing samples and diluting samples and performing distribution according to detection needs; the multifunctional liquid-absorbing needle 2 cooperates with the dilution module to realize quantitative addition of diluent into the detection cup.

[0068] The detection module is used to detect the cell components of the blood sample and the components contained in the plasma. The detection module includes a sample cup position for placing the sample cup and a built-in reagent position for placing the detection reagent. The built-in reagent positions are all located on the movement track of the multifunctional liquid pipetting needle 2.

[0069] The numerical control module controls the operation of the sample processing module and the detection module, and the data processing module processes the detection data.

[0070] Furthermore, the built-in reagent position includes a built-in reagent position for placing a dilution cup 6, a detection position for placing a detection cup 7, and a detection position for placing a reagent bottle. The reagent bottle is used to place an aggregation inducer. The detection cup 7 is provided with a platelet detection device and a stirring device for mixing the diluted sample liquid in the cup. The dilution cup 6 and the sample cup are respectively provided with a stirring device. The lower part of the dilution cup 6 and the detection cup 7 is provided with a drainage port, and the dilution cup 6 and the detection cup 7 are connected to the waste liquid treatment module through the drainage port.

[0071] The waste liquid treatment module includes a third liquid flow control valve 20 and a first liquid discharge pump 13 . The dilution cup 6 and the detection cup 7 are both connected to the third liquid flow control valve 20 via a waste liquid discharge pipe 16 . The third liquid flow control valve 20 is connected to the first liquid discharge pump 13 via a waste liquid discharge collection pipe 17 .

[0072] Furthermore, in some embodiments, the stirring device includes a magnetic stirring device 14 and a magnetic stirring bar 15, wherein the magnetic stirring bar 15 is disposed in the detection cup, and the magnetic stirring device 14 is disposed at the outer bottom of the detection cup. The magnetic stirring device 14 drives the magnetic stirring bar 15 to move by magnetic force to stir the sample.

[0073] The bottom of the dilution cup 6 and the detection cup 7 are respectively provided with radial shallow grooves, which are adapted to the shape of the magnetic stirring bar 15. The magnetic stirring bar 15 falls into the radial shallow grooves after the stirring stops. The magnetic stirring bar 15 is in a stable static state when not stirring.

[0074] Furthermore, in some embodiments, the stirring device is an inflation mixing needle arranged in the dilution cup 6 and the detection cup 7 and an air supply pump connected to the inflation mixing needle, the exhaust port of the inflation mixing needle is respectively located in the dilution cup 6 and the detection cup 7, and the air supply pump provides disturbance gas to the inflation mixing needle to stir and mix the liquid in the dilution cup 6 and the detection cup 7.

[0075] Furthermore, the dilution module includes a diluter 11 and a diluent barrel 10 for placing a diluent; the multifunctional pipette needle 2, the first liquid circuit tube 5, the diluter 11, the second liquid circuit tube 9 and the diluent barrel 10 are connected in sequence, the first liquid circuit tube 5 is provided with a first liquid flow control valve 18, and the second liquid circuit tube 9 is provided with a second liquid flow control valve 19.

[0076] When it is necessary to add diluent to the detection cup through the multifunctional liquid pipette needle 2, the first liquid flow control valve 18 is closed, the second liquid flow control valve 19 is opened, the piston of the diluter 11 is retracted, and a negative pressure is formed in the second liquid circuit tube 9 so that the diluent in the diluent barrel 10 is sucked into the diluter cavity; then the second liquid flow control valve 19 is closed, the first liquid flow control valve 18 is opened, the piston of the diluter 11 is pushed forward, and the diluent sucked in the diluter 11 cavity is passed through the first liquid circuit tube 5 to the multifunctional liquid pipette needle 2 and distributed into the detection cup.

[0077] Embodiment 1:

[0078] The platelet function detection device calculates the platelet function by comparing the changes in the number of platelets before and after adding the aggregating agent to the blood sample. The workflow includes the following steps:

[0079] Step 1: Preliminary preparation: add the loaded blood sample to be tested into the sample cup, and control the stirring device through the CNC module to mix the sample.

[0080] The third liquid flow control valve 20 at the bottom of the dilution cup 6 and the detection cup 7 is opened, and the first liquid discharge pump 13 works to discharge the dilution liquid originally in the dilution cup 6 and the detection cup 7. The multifunctional liquid pipette needle 2 cooperates with the diluter 11 to add a certain amount of dilution liquid to the dilution cup 6 and the detection cup 7 respectively, and the dilution cup 6 and the detection cup 7 are rinsed by the stirring device. After the rinsing is completed, the rinsed dilution liquid is discharged.

[0081] The multifunctional liquid pipetting needle 2 cooperates with the diluter 11 to add a certain amount of diluent into the release cup 6 and the detection cup 7 respectively, and both cups retain the diluent.

[0082] Step 2, dilute the sample, the multifunctional pipette needle 2 moves to the sample cup, the multifunctional pipette needle 2 cooperates with the diluter 11 to absorb a certain amount of blood sample and transfer it to the dilution cup 6, and add a certain amount of diluent to the dilution cup 6, and mix the blood sample in the dilution cup 6 through the stirring device.

[0083] Step 3, original detection, the multifunctional liquid pipette needle 2 absorbs a certain amount of diluted blood sample from the dilution cup 6 and transfers it to the detection cup 7, and adds a certain amount of diluent to the detection cup 7, and mixes the blood sample in the detection cup 7 through the stirring device. The detection cup 7 performs detection on the blood sample in the cup through the detection device 12 to obtain the information data of the original state of the platelets in the blood sample.

[0084] Step 4, waste liquid treatment, the drainage channels at the bottom of the dilution cup 6 and the detection cup 7 are opened, and the waste liquid in the dilution cup 6 and the detection cup 7 is discharged through the connecting pipe and the first drainage pump 13.

[0085] Step 5, cleaning the device, adding a certain amount of diluent to the dilution cup 6 and the detection cup 7 respectively through the cooperation of the multifunctional liquid pipette needle 2 and the diluter 11, and rinsing the dilution cup 6 and the detection cup 7 through the stirring device, and discharging the rinsing diluent after the rinsing is completed.

[0086] Step 6: Add a certain amount of diluent into the release cup 6 and the detection cup 7 respectively through the cooperation of the multifunctional liquid pipetting needle 2 and the diluter 11.

[0087] Step 7, reaction detection, a certain amount of aggregating agent is drawn from the reagent bottle by the multifunctional pipette needle 2 and added to the sample cup for completing the original blood sample detection, the blood sample and the reagent in the sample cup are stirred and mixed by the stirring device, after the reaction time is sufficient, the multifunctional pipette needle 2 draws the reacted blood sample from the sample cup and performs the detection according to the above steps 1-5 to obtain the information after the platelet aggregation reaction in the blood sample treated with the aggregating agent.

[0088] Step 8, data processing, the device compares the platelet number and volume changes of the blood sample obtained by the above process before and after the addition of the aggregation agent to obtain the platelet function level test result of the blood sample;

[0089] Step 9, repeat the test, the device repeatedly executes the platelet function test results of different blood samples according to the above steps 1-8.

[0090] When the platelet function detection device detects more than one blood sample at the same time, the platelet quantity detection of each different original blood sample can be arranged at an optimized time, and the time sequence of adding the aggregation agent can be optimized to achieve optimized process detection of more than one sample, or the detection of each different sample can be completed one by one according to the above steps to obtain different samples, or different platelet receptor function test results for the same sample.

[0091] Embodiment 2:

[0092] The platelet function detection device calculates the platelet function by adding different reagents to the same blood sample to detect the number of platelets obtained. The workflow includes the following steps:

[0093] Step 1, preliminary preparation, add reagents in different reagent bottles to sample cup 101 and sample cup 102 respectively containing the same blood sample for processing, add the strong anticoagulant EDTA in the first reagent bottle 801 to sample cup 101, add the platelet function inducing agent arachidonic acid in the second reagent bottle 802 to sample cup 102, and control the stirring device through the CNC module to mix each sample.

[0094] The third liquid flow control valve 20 at the bottom of the dilution cup 6 and the detection cup 7 is opened, and the first liquid discharge pump 13 works to discharge the dilution liquid originally stored in the dilution cup 6 and the detection cup 7; the multifunctional liquid pipette needle 2 cooperates with the diluter 11 to add a certain amount of dilution liquid to the dilution cup 6 and the detection cup 7 respectively, and the dilution cup 6 and the detection cup 7 are rinsed by the stirring device, and the rinsed dilution liquid is discharged after the rinsing is completed.

[0095] The multifunctional liquid pipetting needle 2 cooperates with the diluter 11 to add a certain amount of diluent into the release cup 6 and the detection cup 7 respectively, and both cups retain the diluent.

[0096] Step 2, dilute the sample, the functional pipette needle 2 moves to the sample cup 101, the multifunctional pipette needle 2 cooperates with the diluter 11 to absorb a certain amount of blood sample and transfer it to the dilution cup 6, and add a certain amount of diluent to the dilution cup 6, and mix the blood sample in the dilution cup 6 through the stirring device.

[0097] Step 3, sample testing, the multifunctional pipette needle 2 draws a certain amount of diluted blood sample from the dilution cup 6 and transfers it to the test cup 7, and adds a certain amount of diluent to the test cup 7, and mixes the blood sample in the test cup 7 through the stirring device. The test cup 7 performs a test on the blood sample in the cup through the detection device 12 to obtain information on the platelet status of the blood sample in the blood cup.

[0098] Step 4, waste liquid treatment, the drainage channels at the bottom of the dilution cup 6 and the detection cup 7 are opened, and the waste liquid in the dilution cup 6 and the detection cup 7 is discharged through the connecting pipe and the first drainage pump 13.

[0099] Step 5, cleaning the device, adding a certain amount of diluent to the dilution cup 6 and the detection cup 7 respectively through the cooperation of the multifunctional liquid pipette needle 2 and the diluter 11, and rinsing the dilution cup 6 and the detection cup 7 through the stirring device, and discharging the rinsing diluent after the rinsing is completed.

[0100] Step 6: Add a certain amount of diluent into the release cup 6 and the detection cup 7 respectively through the cooperation of the multifunctional liquid pipetting needle 2 and the diluter 11.

[0101] Step 7, repeat steps 2-6 to test the blood sample in the sample cup 102, and obtain information on the status of platelets in the blood sample after the same blood sample is treated with different reagents.

[0102] Step 8, based on the difference in the test results of the same blood sample to which a platelet function aggregating agent and a platelet function strong anticoagulant are added, the platelet function level test results of the blood sample are obtained by comparison.

[0103] Step 9, repeat steps 1-8 to detect the platelet function of different blood samples.

[0104] In some embodiments, the dilution module includes two diluters, according to Figure 2 As shown, the dilution module of the device has a diluter 11 and a second diluter 23, wherein the diluter 11 has a larger capacity and is mainly used for sucking and dispensing diluent, while the second diluter 23 has a smaller capacity and is mainly used for sucking and dispensing blood samples and diluted blood samples. The two diluters can be driven by a common motor or by two different motors.

[0105] The multifunctional liquid-absorbing needle tip of the device is also provided with a needle surface cleaning device 25, the specific structure of which is an annular flow cleaning pool arranged outside the multifunctional liquid-absorbing needle.

[0106] The blood analyzer of the present design, when loaded with two diluters, has the following working procedures for aspirating and dispensing samples and built-in reagents, as well as aspirating and dispensing diluents: when the multifunctional liquid aspirating needle is needed to aspirate samples or built-in reagents, or to dilute and mix samples, the first liquid flow control valve 18 is closed, the fifth liquid flow control valve 24 is opened, and the piston of the diluter 23 is withdrawn to aspirate samples, dilute samples or built-in reagents; when the samples and reagents aspirated by the multifunctional sample aspirating needle 2 need to be dispensed, the piston of the diluter 23 is pushed forward, and the samples aspirated by the multifunctional sample aspirating needle 2 are pushed out.

[0107] When it is necessary to absorb and distribute the diluent, first the first liquid flow control valve 18 and the fourth liquid flow control valve 22 are closed, the second liquid flow control valve 19 is opened, the piston of the diluter 11 is retracted, and the diluent is sucked into the inner cavity of the diluter 11 due to the negative pressure formed by the retraction in the diluter 11. Then the second liquid flow control valve 19 is closed, the first liquid flow control valve 18 and the fifth liquid flow control valve 24 are opened, and the piston of the diluter 11 is pushed forward, forming a large positive pressure in the diluter 11 to drive the diluent in the inner cavity of the diluter 11 through the pipeline through the opened first liquid flow control valve 18 and the fifth liquid flow control valve 24, and then discharged through the multifunctional sample suction needle 2 to be distributed to the required target container.

[0108] When the surface of the multifunctional sample suction needle 2 needs to be cleaned, first the first liquid flow control valve 18 and the fourth liquid flow control valve 22 are closed, while the second liquid flow control valve 19 is opened, and the piston of the diluter 11 is retracted to form a negative pressure in the diluter 11, so that the diluent in the diluent barrel 10 is sucked into the inner cavity of the diluter 11 along the pipeline, and then the second liquid flow control valve 19 is closed, the fourth liquid flow control valve 22 is opened, and the piston of the diluter 11 is pushed forward, so that the diluent in the inner cavity of the diluter 11 flows to the water inlet of the multifunctional liquid suction needle surface cleaning device 25 through the pipeline connected to the fourth liquid flow control valve 22 under pressure driving, and at the same time, the third liquid flow control valve 20 is closed, the sixth liquid flow control valve 26 is opened, and the second liquid discharge pump 21 starts to work, so that the diluent entering the multifunctional liquid suction needle surface cleaning device 25 is quickly discharged to achieve the effect of cleaning the surface of the multifunctional liquid suction needle.

[0109] The present invention provides a light-weight platelet function detection device and method. There are many methods and ways to implement the technical solution. The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention. All components not specified in this embodiment can be implemented by existing technologies.

Claims

1. A lightweight platelet function detection device, characterized in that: It includes a sample processing module and a detection module; The sample processing module comprises a multifunctional liquid-absorbing needle (2) for absorbing liquid, a driving device, and a dilution module for diluting the sample; the multifunctional liquid-absorbing needle (2) is connected to the dilution module via a liquid line tube, and the driving device controls the movement of the multifunctional liquid-absorbing needle (2); the multifunctional liquid-absorbing needle (2) cooperates with the dilution module to realize quantitative absorption and distribution of reagents, and at the same time has the functions of quantitatively absorbing samples and diluting samples and executing sample and diluted sample distribution according to detection requirements; the dilution module is connected to the dilution liquid barrel (10) via a pipeline; The detection module is used to detect platelet components in a blood sample, and comprises a sample cup position for placing a sample cup and a built-in reagent position for placing a detection reagent, wherein the sample cup position and the built-in reagent position are both located on the movement track of the multifunctional liquid aspiration needle (2).

2. The lightweight platelet function detection device according to claim 1, characterized in that: The detection module comprises a dilution cup (6) and a detection cup (7), wherein the detection cup (7) is provided with a platelet detection device and a stirring device for mixing the diluted sample liquid in the cup, the dilution cup (6) is provided with a stirring device, and the lower parts of the dilution cup (6) and the detection cup (7) are provided with a liquid discharge port, and the dilution cup (6) and the detection cup (7) are connected to the waste liquid treatment module through the liquid discharge port; The waste liquid treatment module comprises a third liquid flow control valve (20) and a first liquid discharge pump (13); the dilution cup (6) and the detection cup (7) are both connected to the third liquid flow control valve (20) via a waste liquid discharge pipe (16); and the third liquid flow control valve (20) is connected to the first liquid discharge pump (13) via a waste liquid discharge collection pipe (17).

3. The lightweight platelet function detection device according to claim 2, characterized in that: The stirring device comprises a magnetic stirring device (14) and a magnetic stirring bar (15), wherein the magnetic stirring bar (15) is respectively arranged in the dilution cup and the detection cup, and the magnetic stirring device (14) is respectively arranged at the outer bottom of the detection cup and the dilution cup, and the magnetic stirring device (14) drives the magnetic stirring bar (15) to move by magnetic force, thereby stirring the diluted samples in the dilution cup (6) and the detection cup (7); The inner bottoms of the dilution cup (6) and the detection cup (7) are respectively provided with radial shallow grooves, the radial shallow grooves being adapted to the shape of the magnetic stirring bar (15), the magnetic stirring bar (15) falling into the radial shallow grooves after the stirring stops, and the magnetic stirring bar (15) is in a stable static state when not stirring.

4. The lightweight platelet function detection device according to claim 2, characterized in that: The stirring device comprises an inflatable mixing needle arranged in the dilution cup (6) and the detection cup (7) and an air supply pump connected to the inflatable mixing needle. The exhaust port of the inflatable mixing needle is respectively located in the dilution cup (6) and the detection cup (7). The air supply pump provides disturbance gas to the inflatable mixing needle to stir and mix the diluted samples in the dilution cup (6) and the detection cup (7).

5. The lightweight platelet function detection device according to claim 3 or 4, characterized in that: The dilution module comprises a diluter (11) and a diluent barrel (10) arranged outside the device for placing a diluent; the multifunctional liquid pipette (2), the first liquid line tube (5), the diluter (11), the second liquid line tube (9) and the diluent barrel (10) are connected in sequence, the first liquid line tube (5) is provided with a first liquid flow control valve (18), and the second liquid line tube (9) is provided with a second liquid flow control valve (19).

6. A method for platelet function testing using the device of claim 5, characterized in that: The steps include: Step 1, pre-preparation, add the loaded blood sample to be tested and the magnetic stirrer into the sample cup, place the sample cup on the sample cup position of the platelet function detection device, and the device rotates the magnetic stirrer in the sample cup by the magnetic stirring device arranged in the sample cup to rotate and mix the sample in the sample cup; The third liquid flow control valve (20) at the bottom of the dilution cup (6) and the detection cup (7) is opened, and the dilution liquid originally stored in the dilution cup (6) and the detection cup (7) is discharged through the first liquid discharge pump (13), and the multifunctional liquid aspiration needle (2) cooperates with the diluter (11) to add a certain amount of dilution liquid to the dilution cup (6) and the detection cup (7) respectively, and the dilution cup (6) and the detection cup (7) are rinsed by the stirring device. After the rinsing is completed, the rinsed dilution liquid in the dilution cup (6) and the detection cup (7) is discharged; The multifunctional liquid aspirating needle (2) and the diluter (11) cooperate again to add a certain amount of diluent into the release cup (6) and the detection cup (7), respectively, and at this time, the diluent added into the two cups is kept in the cups; Step 2, diluting the sample, the multifunctional liquid pipette needle (2) moves and extends into one of the sample cups, the multifunctional liquid pipette needle (2) cooperates with the diluter (11) to absorb a certain amount of blood sample and transfer it to the dilution cup (6), and a certain amount of diluent is added to the dilution cup (6), and the blood sample in the dilution cup (6) is mixed by the stirring device; Step 3, original detection, the multifunctional liquid aspirating needle (2) draws a certain amount of diluted blood sample from the dilution cup (6) and transfers it to the detection cup (7), and adds a certain amount of diluent to the detection cup (7), and stirs and mixes the blood sample in the detection cup (7) by a stirring device, so that the blood sample is further diluted and mixed, and the blood sample in the detection cup (7) is tested by a detection device (12) to obtain information data of platelets in the original state in the blood sample; Step 4, waste liquid treatment, the drainage channels at the bottom of the dilution cup (6) and the detection cup (7) are opened, and all the waste liquid in the dilution cup (6) and the detection cup (7) is discharged through the connecting pipe and the first drainage pump (13); Step 5, cleaning the device, adding a certain amount of diluent into the dilution cup (6) and the detection cup (7) respectively through the cooperation of the multifunctional liquid pipetting needle (2) and the diluter (11), and stirring and rinsing the dilution cup (6) and the detection cup (7) through the stirring device, and after rinsing, discharging the rinsed dilution liquid in the dilution cup (6) and the detection cup (7); Step 6, adding a certain amount of diluent into the release cup (6) and the detection cup (7) respectively through the cooperation of the multifunctional liquid pipetting needle (2) and the diluter (11); Step 7, reaction detection, a certain amount of aggregating agent is sucked from the reagent bottle by the multifunctional liquid suction needle (2) and added to the sample cup that has completed the original blood sample test, that is, the blood sample cup that has just completed the test, and the blood sample and the reagent in the sample cup are stirred and mixed by the stirring device. After sufficient reaction, the multifunctional liquid suction needle (2) sucks the reacted blood sample from the sample cup and performs the test according to the above steps 1-5 to obtain the information data after the platelet aggregation reaction in the blood sample treated with the aggregating agent; Step 8, data processing, the device compares the platelet counts of the blood sample obtained by the above process before and after the addition of the aggregation agent to obtain the platelet function level test result of the blood sample; Step 9, repeat the test, the device repeatedly executes the platelet function test results of different blood samples according to the above steps 1-8.

7. A method for platelet function testing using the device of claim 5, characterized in that: The device does not have a built-in reagent position for the aggregation agent, and the platelet function detection process includes the following steps: Step 1, pre-preparation, at least two sample cups respectively containing the same amount of blood samples are placed in the sample cup position, and a magnetic stirrer is added to each sample cup, and different reagents are added to each sample cup for treatment, a platelet function inhibitor is added to one sample cup, and a platelet receptor function aggregator is added to the remaining blood samples, and the sample-reagent in each sample cup is stirred and mixed by controlling the stirring device; The third liquid flow control valve (20) at the bottom of the dilution cup (6) and the detection cup (7) is opened, and the dilution liquid originally stored in the dilution cup (6) and the detection cup (7) is discharged through the first liquid discharge pump (13); the multifunctional liquid aspiration needle (2) cooperates with the diluter (11) to add a certain amount of dilution liquid to the dilution cup (6) and the detection cup (7), respectively, and the dilution cup (6) and the detection cup (7) are rinsed by the stirring device, and after the rinsing is completed, the rinsed dilution liquid in the dilution cup (6) and the detection cup (7) is discharged; The multifunctional liquid aspiration needle (2) cooperates with the diluter (11) to add a certain amount of diluent into the release cup (6) and the detection cup (7), respectively, and the diluent added into the two cups is kept in the cups; Step 2, diluting the sample, the functional liquid pipette needle (2) moves and extends into one of the sample cups, the multifunctional liquid pipette needle (2) cooperates with the diluter (11) to absorb a certain amount of blood sample and transfer it to the dilution cup (6), and a certain amount of diluent is added to the dilution cup (6), and the blood sample in the dilution cup (6) is mixed by the stirring device; Step 3, sample testing, the multifunctional liquid aspirating needle (2) draws a certain amount of diluted blood sample from the dilution cup (6) and transfers it to the testing cup (7), and adds a certain amount of diluent to the testing cup (7), and the blood sample in the testing cup (7) is diluted and mixed again by the stirring device, and the testing cup (7) performs testing on the mixed diluted blood sample in the testing cup by the testing device (12), and obtains the platelet count and volume information of the blood sample in the blood sample cup; Step 4, waste liquid treatment, the drainage channels at the bottom of the dilution cup (6) and the detection cup (7) are opened, and the waste liquid in the dilution cup (6) and the detection cup (7) is discharged through the connecting pipe and the first drainage pump (13); Step 5, cleaning the device, adding a certain amount of diluent into the dilution cup (6) and the detection cup (7) respectively through the cooperation of the multifunctional liquid pipetting needle (2) and the diluter (11), and stirring and rinsing the dilution cup (6) and the detection cup (7) through the stirring device, and after rinsing, discharging the rinsed dilution liquid in the dilution cup (6) and the detection cup (7); Step 6, adding a certain amount of diluent to the dilution cup (6) and the detection cup (7) respectively through the cooperation of the multifunctional liquid pipetting needle (2) and the diluter (11), and at this time, the diluent added to the two cups is kept in the cups; Step 7, repeating steps 2-6 to test the blood samples in the remaining sample cups, and obtaining information on the status of platelets in the blood samples after the same blood samples are treated with different reagents; Step 8, comparing and obtaining the platelet function level test result of the blood sample according to the difference in the test results of the same blood sample to which the platelet function aggregator and the platelet function inhibitor are added; Step 9, repeat steps 1-8 to detect the platelet function of different blood samples.

8. The method for platelet function detection according to claim 6 or 7, characterized in that: The platelet function detection device is provided with a cleaning agent reagent position. The platelet function detection device is cleaned when the cleaning conditions are met. The cleaning conditions include before starting the detection every day, after the detection is completed, the device does not perform the detection for more than a specified time, and the user's set conditions. The cleaning steps include: Step S1, a multifunctional liquid aspirating needle absorbs a certain amount of cleaning agent from the cleaning agent bottle and discharges it into the dilution cup and the detection cup respectively, and the cleaning agent is soaked in the dilution cup and the detection cup for a certain period of time; Step S2, stirring the cleaning agent in the dilution cup (6) and the detection cup (7) by means of a stirring device; Step S3, the third liquid flow control valve (20) at the bottom of the dilution cup (6) and the detection cup (7) is opened, and the cleaning agent in the dilution cup (6) and the detection cup (7) is discharged through the first liquid discharge pump (13); Step S4, repeating steps S1-S3 until the cleaning requirements are met.

9. The lightweight platelet function detection device according to claim 1, characterized in that: The sample cup position on the platelet function detection device is provided with a magnetic stirring device, and a magnetic stirring bar is provided in the sample cup.

10. The lightweight platelet function detection device according to claim 1, characterized in that: The sample cup of the platelet function detection device is an independent single cup, or is composed of at least two cups of the same structure to form a single row of combined sample cup structures, and each cup of the combined sample cup is independent of each other and not connected to each other; the height of the sample cup is 12-55mm, and the inner diameter of a single cup is 5-15mm.

Images