Automatic sampling device and method for rapid detection of starch

Abstract

The present invention relates to the field of starch detection, and provides a starch rapid detection automatic sample introduction apparatus and method, the apparatus comprises: a solution vessel for placing a detection solution of each stage in starch detection; the sensing device is arranged in the solution vessel and is used for acquiring liquid level information in the solution vessel; the control equipment comprises reagent dropwise adding equipment, heating equipment, stirring equipment and valve control equipment; the control system is used for acquiring the liquid level information transmitted by the sensing device and driving the control equipment according to a preset program to complete automatic detection of starch; an automatic sample injection and control system is adopted to replace manual operation, the size of a detection instrument can be reduced, and the detection efficiency is improved.

Description

Technical Field

[0001] The invention relates to the field of starch detection, and in particular to an automatic sampling device and method for rapid starch detection. Background Art

[0002] The sugar in the human body mainly comes from the starch in the diet. Testing the starch content can help people control the starch in their diet to achieve the goal of controlling sugar. The current national standard uses ultraviolet spectrophotometry to test the starch content. Its pre-treatment is as follows: Figure 1 As shown, it is necessary to manually prepare the solution, perform heating, stirring, cooling, record the time, and perform corresponding solution addition and mixing operations at the specified time. The pre-treatment operation is complicated and time-consuming. Summary of the invention

[0003] The problem solved by the invention is how to provide an automatic sampling device for rapid starch detection which adopts an automatic sampling and control system to replace manual operation, can reduce the volume of the detection instrument and improve the detection efficiency.

[0004] In order to solve the above problems, the present invention provides an automatic sampling device for rapid starch detection, comprising: a solution container for placing detection solutions at various stages in starch detection;

[0005] A sensor device is arranged in the solution container and is used to obtain liquid level information in the solution container;

[0006] Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment;

[0007] The control system is used to obtain the liquid level information transmitted by the sensor device and drive the control device according to a preset program to complete the automatic detection of starch.

[0008] Furthermore, the solution vessel includes a reaction tank, a detection tank, a waste liquid tank and a sand core filter funnel, the valve control device includes a first solenoid valve and a second solenoid valve, the inlet of the reaction tank is used to add the starch to be detected, the outlet channel of the reaction tank passes through the first solenoid valve and the sand core filter funnel in sequence to reach the inlet of the detection tank, and the outlet channel of the detection tank reaches the inlet of the waste liquid tank through the second solenoid valve.

[0009] Furthermore, the heating device comprises a heating wire, which is used to heat the solution in the reaction tank; the stirring device comprises a stirring rod, which is used to stir the solution in the reaction tank.

[0010] Further, the reagent dropping device comprises a first peristaltic pump, a second peristaltic pump, a third peristaltic pump and a fourth peristaltic pump, and the first peristaltic pump and the second peristaltic pump are connected to the reaction tank;

[0011] The first peristaltic pump is driven by a control system to drop NaOH solution into the reaction tank, and then the control system controls the action of the heating wire and the stirring rod to disperse the starch paste in the reaction tank;

[0012] The second peristaltic pump is driven by a control system to drop a mixed solution of n-butanol-isoamyl alcohol into the reaction tank, followed by heating, stirring and cooling to achieve separation of amylose and amylopectin.

[0013] Furthermore, the third peristaltic pump is connected to the detection pool and driven by the control system to drop iodine reagent into the detection pool to complete the color development detection of the solution in the detection pool;

[0014] The fourth peristaltic pump is connected to the sand core filter funnel. When the reaction is completed and the reaction pool solution flows through the sand core filter funnel, the amylose remains on the sand core filter screen of the sand core filter funnel. The fourth peristaltic pump is used to add formic acid into the sand core filter funnel to dissolve the amylose on the sand core filter screen.

[0015] Further, the sensing device includes a first liquid level sensor, a second liquid level sensor, a third liquid level sensor and a fourth liquid level sensor;

[0016] The first liquid level sensor and the second liquid level sensor are arranged in the reaction tank up and down. During the reaction, the control system obtains the first liquid level information after the NaOH solution is added through the first liquid level sensor, and obtains the second liquid level information after the n-butanol-isoamyl alcohol mixed solution is added through the second liquid level sensor, thereby controlling the closing of the first peristaltic pump and the second peristaltic pump;

[0017] The third liquid level sensor and the fourth liquid level sensor are arranged in the detection pool. When the amylopectin solution enters the detection pool, the third liquid level sensor obtains the third liquid level information. When the amylose enters the detection pool, the fourth liquid level sensor obtains the fourth liquid level information. The control system receives the third liquid level information and the fourth liquid level information, and drives the third peristaltic pump to drip a preset volume of iodine reagent into the detection pool.

[0018] Furthermore, the control system includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

[0019] Furthermore, the input end of the liquid level sensing module is respectively connected to the first liquid level sensor, the second liquid level sensor, the third liquid level sensor and the fourth liquid level sensor, and the output end is respectively connected to the four input ports of the main control chip. The button module includes a first button and a second button, and the first button and the second button are respectively connected to the input end of the main control chip.

[0020] Furthermore, the controlled ends of the first driving chip and the second driving chip are respectively connected to the output ends of the main control module, and the power ends are connected to the power supply module. The output ends of the first driving chip are respectively connected to the heating module, the solenoid valve module and the stirring module. The heating module drives the heating wire, the solenoid valve module drives the first solenoid valve and the second solenoid valve, and the stirring module drives the stirring motor to drive the stirring rod; the four output ends of the second driving chip respectively drive the first peristaltic pump, the second peristaltic pump, the third peristaltic pump and the fourth peristaltic pump.

[0021] The present invention also provides a method for rapid starch detection and automatic sampling, based on the above-mentioned automatic starch detection and automatic sampling device, comprising:

[0022] Put soluble starch into the reaction tank, turn on the power, and give a start signal through the key module;

[0023] The first peristaltic pump motor rotates to add NaOH solution, and the stirring rod starts stirring;

[0024] When the first liquid level information shows that there is water, the first peristaltic pump motor stops rotating, and the heating wire is turned off after heating for a set time;

[0025] The heating stops, the second peristaltic pump motor rotates to drip the n-butanol-isoamyl alcohol mixed solution until the first liquid level information and the second liquid level information both show water, the second peristaltic pump motor stops rotating, and the stirring rod and the heating wire are turned off after working for a set time;

[0026] After the stirring rod and the heating wire are turned off, the first electromagnetic valve is opened, and the solution in the reaction pool enters the detection pool through the first electromagnetic valve and the sand core filter funnel;

[0027] Until the first liquid level information and the second liquid level information both show that there is no water, and the third liquid level information shows that there is water, the first solenoid valve is closed, and the third peristaltic pump motor rotates to drip the iodine reagent for 5 seconds and then stops, completing the pullulan solution detection;

[0028] The main control system makes a judgment based on the information of the key module. If a detection signal of the amylose solution is received, the second solenoid valve is opened for a preset time to allow all the solution in the detection pool to flow into the waste liquid pool, and the second solenoid valve is closed;

[0029] The fourth peristaltic pump motor rotates to drip formic acid solution until the fourth liquid level information shows that there is water, and the fourth peristaltic pump motor stops rotating. The third peristaltic pump motor rotates to drip iodine reagent for 5 seconds and then stops, completing the detection of amylose solution.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] This equipment has low cost and small size, and realizes artificial intelligence and automation of complex processing before starch detection. Compared with manual operation, it greatly improves the detection efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 It is a schematic diagram of the existing manual detection starch pre-treatment process;

[0033] Figure 2 This is a schematic diagram of the structure of an automatic sampling device for rapid starch detection according to an embodiment of the present invention;

[0034] Figure 3 This is a schematic diagram of the circuit principle structure of a control system according to an embodiment of the present invention;

[0035] Figure 4 The figure is a schematic diagram of the process of automated detection of starch pretreatment according to an embodiment of the present invention.

[0036] Description of reference numerals:

[0037] 1-reaction pool; 2-detection pool; 3-waste liquid pool; 4-sand core filter funnel; 5-stirring rod; 6-heating wire; 7-first solenoid valve; 8-second solenoid valve; 9-first liquid level sensor; 10-second liquid level sensor; 11-third liquid level sensor; 12-fourth liquid level sensor; 13-first peristaltic pump; 14-second peristaltic pump; 15-third peristaltic pump; 16-fourth peristaltic pump. DETAILED DESCRIPTION

[0038] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0039] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "set", "install", "connect", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0040] In the description of this specification, the description with reference to the terms "embodiment", "one embodiment" and "one implementation" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or implementation are included in at least one embodiment or implementation of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or implementation. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or implementations in a suitable manner.

[0041] like Figure 2 As shown, the present invention provides an automatic sampling device for rapid starch detection, comprising: a solution container for placing detection solutions at various stages in starch detection;

[0042] A sensor device is arranged in the solution container and is used to obtain liquid level information in the solution container;

[0043] Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment;

[0044] The control system is used to obtain the liquid level information transmitted by the sensor device and drive the control device according to a preset program to complete the automatic detection of starch.

[0045] It should be noted that if Figure 2 As shown, the solution vessel includes a reaction pool 1, a detection pool 2, a waste liquid pool 3 and a sand core filter funnel 4, the valve control device includes a first solenoid valve 7 and a second solenoid valve 8, the outlet channel of the reaction pool 1 sequentially passes through the first solenoid valve 7 and the sand core filter funnel 4 to reach the inlet of the detection pool 2, the outlet channel of the detection pool 2 passes through the second solenoid valve 8 to reach the inlet of the waste liquid pool 3, the heating device includes an electric heating wire 6, the stirring device includes a stirring rod 5, and the stirring rod 5 is driven by a stirring motor, and the reagent dripping device includes a first peristaltic pump 13, a second peristaltic pump 14, The third peristaltic pump 15 and the fourth peristaltic pump 16, the first peristaltic pump 13 and the second peristaltic pump 14 are connected to the reaction pool 1, the third peristaltic pump 15 is connected to the detection pool 2, the fourth peristaltic pump 16 is connected to the sand core filter funnel 4, the sensing device includes a first liquid level sensor 9, a second liquid level sensor 10, a third liquid level sensor 11 and a fourth liquid level sensor 12, the first liquid level sensor 9 and the second liquid level sensor 10 are arranged up and down in the reaction pool 1, and the third liquid level sensor 11 and the fourth liquid level sensor 12 are arranged in the detection pool 2. The operating principle of the system is:

[0046] After starch is added to the reaction tank 1, the first peristaltic pump 13 drips the NaOH solution, and stops dripping when the solution reaches the sensing electrode position of the first liquid level sensor 9, and the stirring rod 5 rotates and the heating wire 6 heats to completely disperse the starch paste;

[0047] The second peristaltic pump 14 drips the n-butanol-isoamyl alcohol mixed solution, and stops when the solution reaches the sensing electrode position of the second liquid level sensor 10, the stirring rod 5 rotates, the heating wire 6 heats, and after cooling, the liquid is amylopectin and the solid is amylose;

[0048] The first electromagnetic valve 7 is opened, and the amylopectin solution is automatically injected into the detection pool 2 after being filtered through the sand core, while the amylose remains on the sand core filter screen. The third peristaltic pump 15 drops iodine reagent for color development, and the detection can be performed;

[0049] The second solenoid valve 8 is opened, and the pullulan solution flows into the waste liquid tank 3 after detection;

[0050] The fourth peristaltic pump 16 drips formic acid to dissolve the amylose solid on the sand core filter and flow into the detection tank 2. After the detection, the second solenoid valve 8 opens and the amylose solution also flows into the waste liquid tank 3.

[0051] In one embodiment of the present invention, to achieve the above-mentioned starch detection, the control system is as follows Figure 3 As shown, it includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

[0052] It should be noted that the main control chip uses a single-chip microcomputer chip U2, whose input port is connected to four liquid level sensors and the first button and the second button, and the output end controls the heating wire 6, the stirring motor, two solenoid valves and four peristaltic pump motors through the driving chips Q1 and Q2; the STC89C52 single-chip microcomputer is used as the control core, and the potential signal change caused by the liquid level sensor causes the high and low potential changes of the single-chip microcomputer control pin, thereby controlling the rotation of the peristaltic pump motor to realize the dripping of reagents, the rotation of the motor drives the glass cup to rotate for stirring, the opening and closing of the solenoid valve, and the heating of the heating wire 6 to achieve the purpose of automatic sampling of branched and amylose. After turning on the power, the initialization program is called, and the button detection program is called cyclically. When the first button and the second button are not pressed, the pins all output high level, and the system has no change. When the first button is pressed, the system will automatically extract the branched starch solution, and then the branched starch detection will extract the branched starch solution by pressing the second button.

[0053] According to the above control system, the overall starch rapid detection automatic sampling method of the present invention is as follows Figure 4 As shown, for the convenience of explanation, the liquid level information at the first liquid level sensor 9, the second liquid level sensor 10, the third liquid level sensor 11 and the fourth liquid level sensor 12 are liquid levels one, two, three and four respectively, including:

[0054] If the first button is pressed, check the liquid level one, two, and three;

[0055] If no water is detected at level 1, 2, or 3, the first peristaltic pump 13 motor rotates to drip NaOH, and the stirring motor rotates to stir;

[0056] If water is detected at level 1, but not at levels 2 and 3, the motor of the first peristaltic pump 13 stops rotating, and the heating wire 6 starts heating;

[0057] After the heating stops, the second peristaltic pump 14 motor rotates to drip isoamyl alcohol;

[0058] If water is detected at level 1 and 2, but not at level 3, the motor of the second peristaltic pump 14 stops rotating and the heating wire 6 starts heating;

[0059] After the heating stops, the stirring is stopped and the first solenoid valve 7 is opened;

[0060] If water is detected at level 3, but not at levels 1 and 2, the first solenoid valve 7 is closed, and the motor of the third peristaltic pump 15 rotates to drip iodine reagent for 5 seconds;

[0061] If both the first button and the second button are pressed, the test continues and the second solenoid valve 8 is opened;

[0062] According to the preset time, the detection liquid will all flow into the waste liquid pool 3, the second solenoid valve 8 is closed, and the fourth peristaltic pump 16 motor rotates to add formic acid;

[0063] If the liquid level 4 detects water, the motor of the fourth peristaltic pump 16 stops rotating.

[0064] The third peristaltic pump 15 motor rotates to drip the iodine reagent and stops after 5 seconds.

[0065] Although the disclosure is disclosed as above, the protection scope of the disclosure is not limited thereto. Those skilled in the art may make various changes and modifications without departing from the spirit and scope of the disclosure, and these changes and modifications will fall within the protection scope of the present invention.

Claims

1. A rapid starch detection automatic sampling device, characterized in that: include: Solution vessels, used to hold the test solutions at various stages of starch testing; A sensor device is arranged in the solution container and is used to obtain liquid level information in the solution container; Control equipment, including reagent adding equipment, heating equipment, stirring equipment and valve control equipment; The control system is used to obtain the liquid level information transmitted by the sensor device and drive the control device according to a preset program to complete the automatic detection of starch.

2. The automatic sampling device for rapid starch detection according to claim 1, characterized in that: The solution vessel comprises a reaction pool (1), a detection pool (2), a waste liquid pool (3) and a sand core filter funnel (4); the valve control device comprises a first solenoid valve (7) and a second solenoid valve (8); the inlet of the reaction pool is used to add the starch to be detected; the outlet channel of the reaction pool reaches the inlet of the detection pool (2) through the first solenoid valve (7) and the sand core filter funnel in sequence; and the outlet channel of the detection pool (2) reaches the inlet of the waste liquid pool (3) through the second solenoid valve.

3. The automatic sampling device for rapid starch detection according to claim 3, characterized in that: The heating device comprises a heating wire (6), which is used to heat the solution in the reaction tank (1); the stirring device comprises a stirring rod (5), which is used to stir the solution in the reaction tank (1).

4. The automatic sampling device for rapid starch detection according to claim 4, characterized in that: The reagent dropping device comprises a first peristaltic pump (13), a second peristaltic pump (14), a third peristaltic pump (15) and a fourth peristaltic pump (16), wherein the first peristaltic pump (13) and the second peristaltic pump are connected to the reaction pool (1); The first peristaltic pump (13) is driven by a control system to drop NaOH solution into the reaction tank (1), and then the control system controls the action of the heating wire (6) and the stirring rod (5) to disperse the starch paste in the reaction tank (1); The second peristaltic pump (14) is driven by a control system to drop a mixed solution of n-butanol and isoamyl alcohol into the reaction pool (1), followed by heating, stirring and cooling to achieve separation of amylose and amylopectin.

5. The automatic sampling device for rapid starch detection according to claim 5, characterized in that: The third peristaltic pump (15) is connected to the detection pool (2) and driven by the control system to drip iodine reagent into the detection pool (2) to complete the color development detection of the solution in the detection pool (2); The fourth peristaltic pump (16) is connected to the sand core filter funnel (4). When the reaction is completed and the solution in the reaction tank (1) flows through the sand core filter funnel (4), the amylose remains on the sand core filter screen of the sand core filter funnel (4). The fourth peristaltic pump (16) is used to add formic acid into the sand core filter funnel (4) to dissolve the amylose on the sand core filter screen.

6. The automatic sampling device for rapid starch detection according to claim 6, characterized in that: The sensing device comprises a first liquid level sensor (9), a second liquid level sensor (10), a third liquid level sensor (11) and a fourth liquid level sensor (12); The first liquid level sensor (9) and the second liquid level sensor (10) are arranged vertically in the reaction tank (1). During the reaction, the control system obtains first liquid level information after the NaOH solution is added dropwise through the first liquid level sensor (9), and obtains second liquid level information after the n-butanol-isoamyl alcohol mixed solution is added dropwise through the second liquid level sensor (10), thereby controlling the closing of the first peristaltic pump (13) and the second peristaltic pump (14); The third liquid level sensor (11) and the fourth liquid level sensor (12) are arranged in the detection pool (2); the third liquid level sensor (11) obtains third liquid level information when the amylopectin solution enters the detection pool (2); the fourth liquid level sensor (12) obtains fourth liquid level information when the amylose enters the detection pool (2); the control system receives the third liquid level information and the fourth liquid level information and drives the third peristaltic pump (15) to drip a preset volume of iodine reagent into the detection pool (2).

7. The automatic sampling device for rapid starch detection according to claim 6, characterized in that: The control system includes a power supply module, a main control chip, a first drive chip, a second drive chip, a liquid level sensing module, a button module, a heating module, a solenoid valve module, a stirring module and a reagent adding module.

8. The automatic sampling device for rapid starch detection according to claim 7, characterized in that: The input end of the liquid level sensing module is respectively connected to a first liquid level sensor (9), a second liquid level sensor (10), a third liquid level sensor (11) and a fourth liquid level sensor (12), and the output end is respectively connected to four input ports of the main control chip. The button module comprises a first button and a second button, and the first button and the second button are respectively connected to the input end of the main control chip.

9. The automatic sampling device for rapid starch detection according to claim 7, characterized in that: The controlled ends of the first driving chip and the second driving chip are respectively connected to the output ends of the main control module, and the power supply ends are connected to the power supply module. The output ends of the first driving chip are respectively connected to the heating module, the solenoid valve module and the stirring module. The heating module drives the heating wire (6), the solenoid valve module drives the first solenoid valve (7) and the second solenoid valve (8), and the stirring module drives the stirring motor to drive the stirring rod (5); the four output ends of the second driving chip respectively drive the first peristaltic pump (13), the second peristaltic pump (14), the third peristaltic pump (15) and the fourth peristaltic pump (16).

10. A method for rapid detection of starch by automatic sampling, based on the automatic sampling device for rapid detection of starch according to any one of claims 1 to 9, characterized in that: include: Put soluble starch into the reaction tank (1), turn on the power, and give a start signal through the key module; The motor of the first peristaltic pump (13) rotates to drop the NaOH solution, and the stirring rod (5) starts stirring; When the first liquid level information indicates that there is water, the motor of the first peristaltic pump (13) stops rotating, and the heating wire (6) is turned off after heating for a set period of time; The heating stops, the motor of the second peristaltic pump (14) rotates to drip the n-butanol-isoamyl alcohol mixed solution until the first liquid level information and the second liquid level information both show the presence of water, the motor of the second peristaltic pump (14) stops rotating, and the stirring rod (5) and the heating wire (6) are turned off after working for a set period of time; After the stirring rod (5) and the heating wire are turned off, the first electromagnetic valve (7) is opened, and the solution in the reaction pool (1) enters the detection pool (2) through the first electromagnetic valve (7) and the sand core filter funnel (4); Until the first liquid level information and the second liquid level information both indicate that there is no water, and the third liquid level information indicates that there is water, the first electromagnetic valve (7) is closed, and the motor of the third peristaltic pump (15) rotates to drip the iodine reagent for 5 seconds and then stops, thereby completing the detection of the amylopectin solution; The main control system makes a judgment based on the information of the key module. If a detection signal of the amylose solution is received, the second solenoid valve (8) is opened for a preset time to allow all the solution in the detection tank (2) to flow into the waste liquid tank (3), and the second solenoid valve (8) is closed; The motor of the fourth peristaltic pump (16) rotates to drip formic acid solution until the fourth liquid level information shows the presence of water, the motor of the fourth peristaltic pump (16) stops rotating, the motor of the third peristaltic pump (15) rotates to drip iodine reagent for 5 seconds and then stops, thus completing the detection of the amylose solution.

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